CN109009235B - A subgingival anaerobic flora collector and method of use - Google Patents

Abstract

The application discloses a subgingival anaerobic flora collector and a using method thereof. The subgingival anaerobic flora collector comprises a cylindrical shell of the collector, a large push rod with a thin needle tube-like structure, a small push rod with a collection wire, a sealing rubber ring, a collection wire, a plastic cover for sealing an outlet, protective gas, an inner ring spring, an outer ring spring and a top pin type telescopic mechanism; one end of the cylindrical shell of the collector is provided with a sealing rubber ring, and the other end of the cylindrical shell of the collector is provided with a plastic cover for sealing an outlet; the plastic cover of the sealing outlet is internally provided with a protection rubber film which is positioned at the bottom of the cylindrical shell of the collector and used for sealing the bottom of the cylindrical shell of the collector. The subgingival anaerobic flora collector provided by the application can ensure that subgingival anaerobic flora is completely protected by the protective gas in the collecting and transferring processes until inoculation, and is effectively combined with the depth range of detection obtained by initial examination, so that bleeding caused by excessive penetration of collecting wires is avoided, and flora collection is more accurate.

Description

A subgingival anaerobic flora collector and method of use

Technical field

The invention relates to the technical field of oral medical devices, in particular to a subgingival anaerobic flora collector and a method of using the collector to complete collection.

Background technique

When studying oral bacterial infections such as periodontal disease, it is usually necessary to collect the flora from specific parts and compare it with the flora in the same parts of normal people to analyze the impact of changes in the number and composition of the flora on the occurrence and development of the disease. effect. The common collection sites for clinical examination are the gingival sulcus and periodontal pockets caused by periodontal disease. The collection methods include paper tip method, standard ring method and inflatable cannula method.

The paper tip method is to select different types of paper tips according to the width of the periodontal pocket, insert it into the bottom of the bag, take it out after 30 seconds, put it into a specimen bottle and transfer it to the laboratory for inoculation. Nitrogen is blown locally in the bottle. It is mainly used for non-adherent subgingival Collection of bacterial flora. However, the paper tip is easily soaked and softened by gingival crevicular fluid, making it difficult to reach the apex of the periodontal pocket where periodontal pathogenic bacteria accumulate.

The standard ring method is to use orthodontic ligation wire to make a collector with a ring diameter of 1 mm similar to a bacterial inoculation ring. The small ring is inserted into the bottom of the periodontal pocket to collect the bacterial flora while blowing nitrogen locally. However, when collecting bacterial flora in the molar area, it is necessary to pull the corner of the mouth and blow nitrogen at the same time, which is inconvenient to operate; and the production quality of the standard ring depends on the doctor. For the gingival sulcus and periodontal pockets of different depths, the diameter of the ring needs to be adjusted. Adjust accordingly and take up clinical time.

The inflatable cannula method uses an injection needle as a cannula with a metal wire core inside. The end of the wire is barbed and covered with calcium alginate. When sampling, the cannula is inserted into the periodontal bag, and then the needle core is pushed out and the bag is collected. flora inside; at the same time, oxygen-free gas is passed through the casing. However, the device is complicated to make, and improper control when the terminal needle core is extended can cause bleeding in the bag and increase the risk of infection.

A common shortcoming of existing collection methods is that they cannot completely guarantee that the collection and transfer process of anaerobic bacteria will be in an anaerobic environment, which will lead to a decrease in the activity of some anaerobic bacteria or even death.

Contents of the invention

The object of the present invention is to provide a subgingival anaerobic bacterial colony collector in view of the above-mentioned existing problems, which can collect bacterial colonies conveniently and quickly while completely ensuring that the collection and transfer process of anaerobic bacterial colonies can be carried out in an oxygen-free environment. , which can lead to reduced activity or even death of some anaerobic bacteria.

The technical solutions adopted by the present invention are as follows:

A subgingival anaerobic flora collector, including a collector cylindrical shell (1), a large push rod with a fine needle tube-like structure (2), a small push rod with a collection wire (3), and a sealing rubber ring (4 ), collection wire (5), sealed outlet cover (6), protective gas (7), inner ring spring (8a), outer ring spring (8b) and top-pin telescopic mechanism (9);

The cylindrical shell (1) of the collector is provided with a sealing rubber ring (4) at one end, and a sealing outlet cover (6) at the other end; the sealing outlet cover (6) is provided with a protective rubber film, and the sealing outlet cover (6) and its rubber membrane is located at the bottom of the cylindrical shell (1) of the collector and is used to seal the bottom of the cylindrical shell (1) of the collector;

The large push rod (2) with a fine needle tube-like structure is provided with a first push plate (2c) at one end and a needle (2a) at the other end. The large push rod (2) with a fine needle tube-like structure is slidably arranged. in the cylindrical housing (1) of the collector, so that the large push rod (2) with a fine needle tube-like structure can reciprocate in the cylindrical housing (1) of the collector with the help of external force;

One end of the small push rod (3) with collection wire is fixedly provided with a collection wire (5), and the other end is provided with a second push plate (3b). The small push rod (3) with collection wire is slidably provided on the belt. In the large push rod (2) with a fine needle tube-like structure, the small push rod (3) with the collection wire can reciprocate in the large push rod (2) with a fine needle tube-like structure with the help of external force.

A zero scale line (2b) and a number of evenly distributed black scale lines (3a) are provided on the outer wall of one end of the needle (2a) and the large push rod (2) with a fine needle tube-like structure. The zero scale line (2b) is located It is the horizontal position where the fixed connection between the large push rod (2) and the needle (2a) is located, and it is also the horizontal position where the fixed connection between the small push rod (3) and the collection wire (5) is located.

After opening the sealed outlet cover (6), the needle (2a) can be pushed out of the rubber membrane through the large push rod (2) with a fine needle tube-like structure.

A closed space is formed between the inner wall of the cylindrical shell (1) of the collector and the outer wall of the large push rod (2) with a fine needle-like structure. The closed space is filled with protective gas (7) to ensure subgingival anaerobic The bacterial colonies are completely protected by protective gas during collection, transportation and inoculation. Further, the filled protective gas (7) is nitrogen.

A ejector pin telescopic mechanism (9) is provided between the large push rod (2) with a fine needle tube-like structure and the small push rod (3) with a collection wire. The ejector pin telescopic mechanism (9) is located on the cylinder of the collector. Inside the shaped shell (1), and installed on the outer wall of the small push rod (3) with the collection wire, close to the sealing rubber ring (4), the large push rod (2) with a fine needle tube-like structure and the small push rod with the collection wire The push rod (3) realizes that the large push rod and the small push rod are pushed out at the same time through the push-pin telescopic mechanism (9) and locked in the extended state, and return to the initial position after being pressed again.

The inner ring spring (8a) is located inside the large push rod (2) with a fine needle tube-like structure, its lower end is fixedly connected with the inner wall of the large push rod (2) with a fine needle tube-like structure, and its upper end is connected with the small push rod (2) with a collection wire. The outer wall of the push rod (3) is fixedly connected;

The outer ring spring (8b) is located inside the cylindrical shell (1) of the collector. Its upper end is fixedly connected to the large push rod (2) with a fine needle-like structure at the zero scale line (2b), and its lower end is connected to the cylindrical shell of the collector. The bottom end of the inner wall of the housing (1) is fixedly connected.

After pressing the second push plate (3b) of the small push rod (3) with the collection wire, the outer ring spring (8b) is first compressed until the front end needle (2a) completely penetrates the rubber membrane, and then continues to press the second push plate (3b). Disk (3b) until the inner spring (8a) is completely compressed, and the collection wire (5) is completely extended from the needle (2a); after letting go, the inner spring (8a) and outer spring (8b) return to their natural state. The needle (2a) and the collection wire (5) are both retracted to their initial positions.

The collection wire (5) has a villi-like open structure, and the sealing outlet cover (6) has a sealant with a thickness of 1 mm.

A method for collecting subgingival anaerobic flora using any subgingival anaerobic flora collector according to claims 1 to 9, the method comprising the following steps:

First, remove the sealed outlet cover (6) on the front part of the collector, hold the body of the collector, align the outlet of the collection wire (5) with the gingival sulcus or periodontal pocket, and push the large push rod (2) with a fine needle-like structure. Push the needle (2)a to pass through the rubber membrane. When the needle (2a) is pushed out by the large push rod (2) with a fine needle tube-like structure, the large push rod (2) with a fine needle tube-like structure will slowly be revealed. 2) The zero scale mark (2b) and the black scale mark (3a) on the outer wall;

During collection, the needle (2a) is positioned by observing the exposed scale value. When the exposed scale value reaches a certain value, the anaerobic bacteria in the gingival sulcus or periodontal pocket are collected. At this time, the pin-type telescopic mechanism is used to collect the needle (2a). (9) Lock the positioned needle (2a);

Continue to push the small push rod (3) with the collection wire to drive the collection wire (5) at the front end of the thin rod-shaped structure to continue to extend from the needle (2a) and into the gingival sulcus or periodontal pocket. Make sure it extends into the sulcus or periodontal pocket. Depth within the bag;

The villi-like structure on the collection wire (5) absorbs the bacteria accumulated in the groove or bag. After 30 seconds, press the small push rod (3) with the collection wire firmly and release it. First, return the small push rod with the collection wire. Rod (3), then withdraw the large push rod (2) with a fine needle tube-like structure, and cover the sealed outlet cover (6) to complete the collection of subgingival anaerobic bacteria.

In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are:

1. The subgingival anaerobic flora collector is a finished collector that provides an anaerobic environment, ensuring that the subgingival anaerobic flora is completely protected by nitrogen during the collection, transfer, and inoculation processes.

2. This collector can quantitatively control the depth of the collection wire into the gingival sulcus or periodontal pocket, effectively combining it with the probing depth range obtained during the initial examination to avoid bleeding caused by excessive penetration of the collection wire.

3. The collector can easily extend into the gingival sulcus or the apex of periodontal pockets where bacteria gather, making the collection of bacteria more accurate.

4. The collector can save the collected bacterial flora in a short time to ensure the quality of the bacterial flora.

Description of the drawings

Figure 1 is a schematic side structural diagram of a device provided by an embodiment of the present invention;

Figure 2 is a schematic front structural view of the device provided by an embodiment of the present invention;

The reference symbols in the figure are as follows: 1—cylindrical shell of the collector, 2—large push rod with fine needle tube-like structure, 3—small push rod with collection wire, 4—sealing rubber ring, 5—collection wire, 6 — Sealed outlet cover, 7 — protective gas, 8a — inner ring spring, 8b — outer ring spring, 9 — ejector pin telescopic mechanism, 2a — needle, 2b — zero mark, 2c — first push plate, 3a — black Graduation mark, 3b—Second push plate.

Detailed ways

In order to enable those in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention are clearly and completely described below in conjunction with the accompanying drawings of the present invention. Based on the embodiments in this application, those of ordinary skill in the art will Other similar embodiments obtained without creative work shall fall within the scope of protection of this application.

Example 1

As shown in Figures 1 to 2, a subgingival anaerobic bacteria collection device includes a cylindrical shell of the collection device 1, a large push rod with a fine needle-like structure 2, a small push rod with a collection wire 3, and a sealing rubber Ring 4, collection wire 5, sealed outlet cover 6, the collector is filled with protective gas 7, inner ring spring 8a, outer ring spring 8b and ejector pin telescopic mechanism 9; one end of the cylindrical shell 1 of the collector is provided with a seal The other end of the rubber ring 4 is provided with a plastic sealing outlet cover 6 with a rubber film that seals the outlet. The large push rod 2 with a thin needle tube-like structure is provided with a first push plate 2c at one end and a needle 2a at the other end. The outer wall of one end of the needle 2a and the large push rod 2 with a thin needle tube-like structure is provided with a zero scale mark 2b and a uniformly distributed black scale mark 3a. The position of the zero scale mark 2b is where the fixed connection between the large push rod 2 and the needle head 2a is located. The horizontal position is also the horizontal position where the small push rod 3 and the collection wire 5 are fixedly connected. The sealed outlet cover 6 is provided with a protective rubber film. The sealed outlet cover 6 and its rubber film are located at the bottom of the cylindrical shell 1 of the collector and are used to seal the bottom of the cylindrical shell 1 of the collector. After opening the sealed outlet cover 6, the needle 2a can be pushed out of the rubber membrane through the large push rod 2 with a fine needle tube-like structure; the large push rod 2 with a fine needle tube-like structure is slidably arranged in the cylindrical housing 1 of the collector. , so that the large push rod 2 with a fine needle tube-like structure can reciprocate in the cylindrical shell 1 of the collector with the help of external force; the small push rod 3 with a collection wire is fixedly provided with a collection wire 5 at one end and a collection wire 5 at the other end. There is a second push plate 3b, and the small push rod 3 with the collection wire is slidably arranged in the large push rod 2 with a fine needle tube-like structure, and with the help of external force, the small push rod 3 with the collection wire can be moved in the small push rod 3 with the fine needle tube The large push rod 2 of the same structure moves reciprocally. A closed space is formed between the inner wall of the cylindrical shell 1 of the collector and the outer wall of the large push rod 2 with a fine needle-like structure. The protective gas 7 is filled into the closed space to ensure the collection and transfer of subgingival anaerobic bacteria. Until the inoculation process, it is completely protected by protective gas. In one embodiment, the filling gas 7 is nitrogen.

A ejector pin telescopic mechanism 9 is provided between the large push rod 2 with a fine needle tube-like structure and the small push rod 3 with a collection wire. The ejector pin telescopic mechanism 9 is located inside the cylindrical shell 1 of the collector and is installed on the collector. One end of the outer wall of the small push rod 3 with the collection wire is close to the sealing rubber ring 4. The large push rod 2 with a fine needle tube-like structure and the small push rod 3 with the collection wire realize the connection between the large push rod and the small push rod through the push-pin telescopic mechanism 9. The push rod is pushed out at the same time and locked in the extended state, and returns to the initial position after being pressed again.

The push-pin telescopic mechanism 9 is an automatic locking structure similar to a ballpoint pen. It is installed on the inside of the large push rod 2 with a fine needle tube-like structure and on the outer wall of the small push rod 3 with a collection wire, and is close to the sealing rubber ring 4. The push pin The telescopic mechanism 9 is composed of an indexing drum, a sliding claw sleeve, and a spring. The sliding claw sleeve has a sliding claw at the front end, and a plurality of indexing claws at the rear end of the indexing drum. There are deep grooves and shallow grooves between the indexing claws. The seed grooves are arranged in a staggered manner. The large push rod 2 with a fine needle tube-like structure and the small push rod 3 with the collecting wire are pushed out at the same time through the push-pin telescopic mechanism 9 and then locked in the extended state. Function to return after pressing again.

The inner ring spring 8a is located inside the large push rod 2 with a fine needle tube-like structure, its lower end is fixedly connected to the inner wall of the large push rod 2 with a fine needle tube-like structure, and its upper end is fixed to the outer wall of the small push rod 3 with a collection wire. Connection; the outer ring spring 8b is located inside the cylindrical shell 1 of the collector, its upper end is fixedly connected to the large push rod 2 with a fine needle tube-like structure at the zero scale line 2b, and its lower end is connected to the bottom end of the inner wall of the cylindrical shell 1 of the collector Fixed connection.

When in use, after pressing the second push plate 3b of the small push rod 3 with the collection wire, the outer ring spring 8b is first compressed until the front end needle 2a is completely penetrated from the rubber film, and then continues to press the second push plate 3b until the inner ring The spring 8a is fully compressed, and the collection wire 5 is completely extended from the needle 2a; after letting go, the inner spring 8a and the outer spring 8b return to their natural state respectively, and the needle 2a and the collection wire 5 are retracted to their initial positions.

In one embodiment, the cylindrical housing 1 of the collector has a total length of 20 mm, an outer diameter of 4 mm, and an inner diameter of 3 mm. The front end is closed by a sealed outlet cover 6 with a thickness of 1 mm and its rubber film, and the other end has an outer diameter of 3 mm and an inner diameter of 2.5 mm. , the sealing rubber ring 4 with a thickness of 1mm is fixed and closed;

In one embodiment, the large push rod 2 with a thin needle tube-like structure has a total length of 25 mm, an outer diameter of 2.5 mm, an inner diameter of 1.5 mm, and a front needle 2a of 5 mm. After it is fully extended, it can move outward at the zero scale line 2b of the housing. The needle tip is exposed 5mm, the diameter of the first push plate 2c is 5mm, the large push rod 2 with a fine needle tube-like structure extends into the part of the cylindrical shell 1 of the collector, and the small push rod 3 with the collection wire penetrates into the part with a fine needle tube-like structure The part of the large push rod 2 is fixed and closed by a sealing rubber ring 4 composed of an outer diameter of 3mm, an inner diameter of 2.5mm, a thickness of 1mm, and an outer diameter of 1.5mm and an inner diameter of 1mm;

In one embodiment, the total length of the small push rod 3 with collection wire is 30mm, the diameter is 1mm, the front end collection wire 5 is 5mm long, and there is a black scale line 3a on the thin rod, marked from "0" to "5" mm , when the small push rod 3 with the collection wire is not moving, the "0" mark line is flush with the zero scale line 2b on the large push rod 2 with a fine needle tube-like structure, and the second push at one end of the small push rod 3 with the collection wire The diameter of the disk 3b is 2.5mm; there is a ejector pin type telescopic mechanism 9 at the junction of the small push rod 3 with the collection wire and the large push rod 2 with a fine needle tube-like structure.

In one embodiment, the collection wire 5 is 5 mm long and has a villi-like open structure on it.

In one embodiment, the sealed outlet cover 6 has a sealant with a thickness of 1 mm, so that the cylindrical housing 1 of the collector can be tightly covered.

Example 2

This embodiment is used to specifically explain the use method of the collector obtained based on any of the previous embodiments:

First, remove the sealed outlet cover 6 at the front of the collector, hold the body of the collector, align the outlet of the collection wire 5 with the healthy gingival sulcus or periodontal pocket of the affected tooth, push the large push rod 2 with a fine needle tube-like structure, and at the same time push the needle 2a When the needle 2a is pushed out from the rubber membrane by the large push rod 2 with a fine needle tube-like structure, the zero mark 2b and other marks on the outer wall of the large push rod 2 with a fine needle tube-like structure will be slowly revealed. Black scale line 3a. During routine collection, you need to locate the needle 2a by observing the exposed scale value. Generally, the exposed scale value is 2 mm, which can achieve the detection of anaerobic bacteria in the gingival sulcus or periodontal pocket of the affected tooth. Collect, and then lock the 2mm positioned needle 2a through the push-pin telescopic mechanism 9; continue to push the small push rod 3 with the collection wire, driving the collection wire 5 at the front end of the thin rod-shaped structure to continue to extend from the needle 2a and Stretch into the gingival sulcus or periodontal pocket, and determine the depth into the sulcus or pocket according to the scale on the small push rod 3 that pushes the collection wire; the villi-like structure on the collection wire 5 absorbs the inside of the sulcus or pocket, especially the root. For the bacteria collected at the tip, after 30 seconds, press the small push rod 3 with the collection wire firmly and then release it. First, return the small push rod 3 with the collection wire, and then return the large push rod 2 with the fine needle tube-like structure. Cover the sealed outlet cover 6 to complete the collection of subgingival anaerobic bacteria.

The present invention is not limited to the specific embodiments described above. The invention extends to any new features or any new combinations disclosed in this specification, as well as to any new method or process steps disclosed or any new combinations.

All features disclosed in this specification, or all steps in a method or process disclosed, except mutually exclusive features and/or steps, may be combined in any way.

This specification includes any feature disclosed in any appended claims and abstract. Unless specifically stated, any feature disclosed may be replaced by other equivalent features or alternative features with similar purposes. That is, unless otherwise stated, each feature is only one example in a series of equivalent or similar features.

Claims (10)

1. The subgingival anaerobic flora collector is characterized by comprising a cylindrical collector shell (1), a large push rod (2) with a thin needle tube-like structure, a small push rod (3) with a collection wire, a sealing rubber ring (4), a collection wire (5), a sealing outlet cover (6), protective gas (7), an inner ring spring (8 a), an outer ring spring (8 b) and a top pin type telescopic mechanism (9); the inner ring spring (8 a) is positioned at the inner side of the large push rod (2) with the thin needle tube-like structure, and the outer ring spring (8 b) is positioned at the inner side of the cylindrical shell (1) of the collector;

one end of the cylindrical shell (1) of the collector is provided with a sealing rubber ring (4), and the other end of the cylindrical shell is provided with a sealing outlet cover (6); a protection rubber film is arranged in the sealing outlet cover (6), and the sealing outlet cover (6) and the rubber film thereof are positioned at the bottom of the cylindrical shell (1) of the collector and are used for sealing the bottom of the cylindrical shell (1) of the collector;

a closed space is formed between the inner wall of the cylindrical shell (1) of the collector and the outer wall of the large push rod (2) with the thin needle tube-like structure, and protective gas (7) is filled in the closed space; a top pin type telescopic mechanism (9) is arranged between the large push rod (2) with the thin needle tube sample structure and the small push rod (3) with the collection wire;

one end of the large push rod (2) with the thin needle tube-like structure is provided with a first push disc (2 c), the other end of the large push rod (2) with the thin needle tube-like structure is provided with a needle head (2 a), and the large push rod (2) with the thin needle tube-like structure is arranged in the cylindrical shell (1) of the collector in a sliding manner, so that the large push rod (2) with the thin needle tube-like structure can reciprocate in the cylindrical shell (1) of the collector under the help of external force;

the small push rod (3) with the collection wire is fixedly provided with the collection wire (5) at one end, the second push plate (3 b) is arranged at the other end, the small push rod (3) with the collection wire is arranged in the large push rod (2) with the thin needle tube sample structure in a sliding mode, and the small push rod (3) with the collection wire can reciprocate in the large push rod (2) with the thin needle tube sample structure by means of the small push rod (3) with the collection wire under external force.

2. The subgingival anaerobic flora collector of claim 1, wherein zero graduation marks (2 b) and a plurality of uniformly distributed black graduation marks (3 a) are arranged on the outer wall of one end of the needle head (2 a) and the large push rod (2) with the thin needle tube-like structure, and the position of the zero graduation marks (2 b) is the horizontal position of the fixed connection part of the large push rod (2) and the needle head (2 a) and the horizontal position of the fixed connection part of the small push rod (3) and the collecting wire (5).

3. A subgingival anaerobic flora harvester according to claim 1, in which the needle (2 a) is pushed out of the rubber membrane by a large push rod (2) with a thin needle-like structure after opening the sealing outlet cover (6).

4. A subgingival anaerobic flora harvester according to claim 1, wherein subgingival anaerobic flora is ensured to be completely protected by a protective gas during harvesting and transport until inoculation.

5. A subgingival anaerobic flora harvester according to claim 4, in which the filled shielding gas (7) is nitrogen.

6. A subgingival anaerobic flora harvester according to claim 1, characterized in that the ejector pin type telescopic mechanism (9) is located inside the cylindrical housing (1) of the harvester, and is mounted on one end of the outer wall of the small push rod (3) with the collection wire, which is close to the sealing rubber ring (4), and the large push rod (2) with the thin needle tube type structure and the small push rod (3) with the collection wire are locked in an extending state after being pushed out simultaneously by the ejector pin type telescopic mechanism (9), and are retracted to the initial position after being pressed again.

7. A subgingival anaerobic flora collector according to claim 2, characterized in that the lower end of the inner ring spring (8 a) is fixedly connected with the inner wall of the large push rod (2) with the thin needle tube-like structure, and the upper end is fixedly connected with the outer wall of the small push rod (3) with the collection wire;

the upper end of the outer ring spring (8 b) is fixedly connected with the large push rod (2) with the thin needle tube-like structure at the position of the zero scale mark (2 b), and the lower end of the outer ring spring is fixedly connected with the bottom end of the inner wall of the cylindrical shell (1) of the collector.

8. A subgingival anaerobic flora harvester according to claim 7, in which after pressing the second push plate (3 b) of the small push rod (3) with the harvesting wire, the outer ring spring (8 b) is compressed until the front needle (2 a) is completely penetrated out of the rubber membrane, and then the second push plate (3 b) is continuously pressed until the inner ring spring (8 a) is completely compressed, and the harvesting wire (5) is completely extended out of the needle (2 a); after the hands are loosened, the inner ring spring (8 a) and the outer ring spring (8 b) are respectively restored to the natural state, and the needle head (2 a) and the collection wire (5) are retracted to the initial positions.

9. A subgingival anaerobic flora harvester according to claim 8, wherein said harvesting filaments (5) have a villiated open structure, and said sealed outlet cover (6) has a 1mm thick sealant therein.

10. A method of harvesting using a subgingival anaerobic flora harvester of any one of claims 1-9, comprising the steps of:

firstly, a sealed outlet cover (6) at the front section of the collector is taken down, the body of the collector is held by hands, the outlet of a collection wire (5) is aligned with a gingival sulcus or periodontal pocket, a large push rod (2) with a thin needle tube-like structure is pushed, a needle head (2 a) is pushed out from a rubber membrane, and zero scale marks (2 b) and black scale marks (3 a) on the outer wall of the large push rod (2) with the thin needle tube-like structure are slowly exposed in the process of pushing out the needle head (2 a) by the large push rod (2) with the thin needle tube-like structure;

the needle head (2 a) is positioned by observing the exposed scale values during collection, anaerobic flora of gingival sulcus or periodontal pocket is collected when the exposed scale values reach a certain value, and the positioned needle head (2 a) is locked by the ejector pin type telescopic mechanism (9);

the small push rod (3) with the collection wire is continuously pushed, the collection wire (5) at the front end of the thin rod-shaped structure is driven to continuously extend out of the needle head (2 a) and extend into the gingival sulcus or periodontal pocket, and the depth of the collection wire extending into the sulcus or pocket is determined;

the villus structure on the collection silk (5) adsorbs the flora gathered in the ditch or the bag, and after 30 seconds, the small push rod (3) with the collection silk is pressed down by force and then loosened, the small push rod (3) with the collection silk is retracted first, then the large push rod (2) with the fine needle tube sample structure is retracted, and the outlet cover (6) is covered to complete the collection operation of the subgingival anaerobic flora.