CN109005967A - A kind of Chinese catalpa cottage method and Chinese catalpa seedling - Google Patents
A kind of Chinese catalpa cottage method and Chinese catalpa seedling Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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Abstract
Description
技术领域technical field
本发明涉及林木繁殖技术领域,具体而言,涉及一种楸树扦插方法及楸树苗。The invention relates to the technical field of tree propagation, in particular to a catalpa tree cutting method and catalpa saplings.
背景技术Background technique
楸树的树干通直,枝叶浓密,树体华丽如冠,材质特别优良,生长非常迅速,被誉为“木王”。Catalpa has a straight trunk, dense branches and leaves, and the tree is as gorgeous as a crown. Its material is particularly fine and it grows very fast. It is known as the "King of Wood".
目前楸树已在很多省份推广种植,其在防止水土流失、保护农田、营造用材林、庭院观赏以及道路绿化等方面具有重要的应用价值。目前楸树还没有形成规模化和产业化种植。主要原因是楸树由于楸树自花不亲和性,结实稀少,实生繁殖困难,嫁接繁殖又容易出现不亲和性、早衰等不利情况。繁殖苗木大多采用扦插和组培两种方法,但无论硬枝还是嫩枝,扦插成活率都很低,而组织培养成本高昂,技术复杂繁琐。At present, catalpa has been popularized and planted in many provinces, and it has important application value in preventing soil erosion, protecting farmland, building timber forests, garden viewing and road greening. Catalpa has not yet formed large-scale and industrialized planting. The main reason is that due to the incompatibility of the catalpa tree's self-flowering, the fruit is scarce, the seed propagation is difficult, and the graft propagation is prone to disadvantages such as incompatibility and premature aging. Two methods of cutting and tissue culture are mostly used for the propagation of seedlings, but the survival rate of cuttings is very low regardless of hard branches or tender branches, and the cost of tissue culture is high, and the technology is complicated and cumbersome.
发明内容Contents of the invention
本发明的目的在于提供一种楸树扦插方法,其能够提高楸树扦插的成活率。The object of the present invention is to provide a kind of catalpa cutting method, it can improve the survival rate of catalpa cutting.
本发明的另一目的在于提供一种楸树苗,其培养成本较低,培养步骤较为简洁。Another object of the present invention is to provide a catalpa sapling, which has lower cultivation cost and simpler cultivation steps.
本发明解决其技术问题是采用以下技术方案来实现的。The present invention solves its technical problems by adopting the following technical solutions.
本发明提出一种楸树扦插方法,其主要包括以下步骤:The present invention proposes a kind of catalpa cuttage method, and it mainly comprises the following steps:
将楸树插穗的顶端插入第一培养基质,待楸树插穗的底端的切口有愈伤组织形成或有根原基出现,再将楸树插穗的底端插入第二培养基质。Insert the top of the catalpa cuttings into the first culture substrate, wait for callus formation or root primordia to appear at the incision at the bottom of the catalpa cuttings, then insert the bottom of the catalpa cuttings into the second culture substrate.
本发明提出一种楸树苗,该楸树苗由上述楸树扦插方法培养而得。The invention provides a catalpa sapling, which is cultivated by the above-mentioned catalpa cutting method.
本发明实施例提供的楸树扦插方法、楸树苗的有益效果是:The beneficial effect of the Chinese catalpa cuttage method that the embodiment of the present invention provides, catalpa sapling is:
传统的楸树扦插培育过程中,插穗极易出现先发芽后长根的假活现象,使培育人员不能及时作出相应处理,插穗最后真正成活的数量也就较少。本发明实施例提供的楸树扦插方法中,楸树插穗采用先倒插的方式,避免了传统过程中由假活现象带来的损失。楸树插穗准备好后,将楸树插穗的顶端插入第一培养基质,此时楸树插穗处于倒插的状态下。待楸树插穗的底端的切口有愈伤组织形成或有根原基出现后,再取出楸树插穗,并将楸树插穗的底端插入第二培养基质内进行后续培养。倒插操作简单,人工成本低廉,同时避免了假活现象的出现,有效解决了楸树插穗易腐烂造成难生根的问题。由上述方法培育得到的楸树苗,不仅生命力稳定,且培育成本低廉,适合批量培育。In the process of traditional catalpa cutting cultivation, the cuttings are prone to false liveness that germinates first and then grows roots, so that the cultivators cannot deal with it in time, and the number of cuttings that actually survive is relatively small. In the catalpa cuttage method provided in the embodiment of the present invention, the catalpa cuttings are inserted upside down first, which avoids the loss caused by false work in the traditional process. After the catalpa cuttings are ready, the top of the catalpa cuttings is inserted into the first culture substrate, and the catalpa cuttings are in the state of upside-down insertion. After the cut of the bottom of the catalpa cuttings has callus formation or root primordia appear, the catalpa cuttings are taken out, and the bottom of the catalpa cuttings is inserted into the second culture medium for subsequent culture. The reverse insertion operation is simple, the labor cost is low, and the phenomenon of false work is avoided at the same time, which effectively solves the problem that the cuttings of catalpa are easy to rot and cause difficult rooting. The catalpa saplings cultivated by the above method not only have stable vitality, but also have low cultivation cost and are suitable for batch cultivation.
具体实施方式Detailed ways
为使本发明实施例的目的、技术方案和优点更加清楚,下面将对本发明实施例中的技术方案进行清楚、完整地描述。实施例中未注明具体条件者,按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者,均为可以通过市售购买获得的常规产品。In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.
下面对本发明实施例提供的楸树扦插方法和由该方法培育的楸树苗进行具体说明。The catalpa tree cutting method provided by the embodiments of the present invention and the catalpa tree seedlings cultivated by the method are described in detail below.
本发明实施例提供一种楸树苗,其培育方法主要包括以下步骤:The embodiment of the present invention provides a kind of catalpa sapling, and its cultivation method mainly comprises the following steps:
利用一种楸树扦插方法培育上述楸树苗。扦插繁殖是利用植物细胞的全能性,依靠母体植株上切离下来的根、茎、叶等营养体,在一定的条件下重新发育为一棵完整的新植株。扦插按其选取材料的不同可划分为硬枝扦插、嫩枝扦插、根插、芽插和叶插等形式。其中,以植物半木质化的嫩枝为材料的嫩枝扦插方式因其具有操作简单、苗木生长快、繁殖系数高、成本低等优点,日益受到人们的重视。加之现代日光温室技术的发展,使得嫩枝扦插在植物的整个生长期中都可进行。硬枝扦插繁殖的季节性强,一般都在惊蛰到清明之间进行扦插繁殖。一般来说,嫩枝比较软,出根比较难,硬枝比较成熟特别是一年生的枝条,出根率比较高。为统一描述,本发明实施例中主要采用硬枝进行后续试验以及相关说明。Utilize a kind of catalpa cuttage method to cultivate above-mentioned catalpa sapling. Cutting propagation is to use the totipotency of plant cells and rely on the roots, stems, leaves and other vegetative bodies cut off from the mother plant to re-develop into a complete new plant under certain conditions. Cuttings can be divided into hard branch cuttings, tender branch cuttings, root cuttings, bud cuttings and leaf cuttings according to the different materials selected. Among them, the twig cutting method using the semi-lignified twigs of plants as materials has been increasingly valued by people because of its advantages such as simple operation, fast growth of seedlings, high reproduction coefficient, and low cost. Coupled with the development of modern solar greenhouse technology, twig cuttings can be carried out throughout the growth period of plants. The seasonality of hard branch cutting propagation is strong, and cutting propagation is generally carried out between Jingzhe and Qingming. Generally speaking, tender branches are softer and more difficult to root out, while hard branches are more mature, especially annual branches, which have a higher rooting rate. For a unified description, hard branches are mainly used in the embodiments of the present invention for subsequent tests and related descriptions.
本发明实施例提供的楸树扦插方法主要包括以下步骤:The catalpa cuttage method that the embodiment of the present invention provides mainly comprises the following steps:
准备好楸树插穗。Prepare the catalpa cuttings.
在圃地向阳的地方挖一浅沟,沟深18cm(根据具体需求也可以是其他数值),长、宽视楸树插穗的尺寸而定。当然,该浅沟也可以是现成的向阳的种植沟。在种植沟的底部铺设3-5厘米厚的第一砂层,再将楸树插穗的顶端插入第一砂层,此时即为倒插。再在种植沟内铺设2-4厘米厚的第二砂层,使第二砂层覆盖楸树插穗的底端。需要说明,楸树插穗可以是单个插入也可以是成捆插入。第一砂层、第二砂层不易过厚或过薄,以保证第二砂层具有适宜的通风能力、防止水分散失能力以及保温能力。为保证砂层具有适宜的上述能力,一般将第一砂层、第二砂层浇湿但不透(即砂可以达到手握成团,一触即散的状态)。砂层即作为楸树插穗的第一培养基质。Dig a shallow ditch in the sunny place of the nursery, the ditch depth is 18cm (it can also be other values according to specific needs), and the length and width depend on the size of the catalpa cuttings. Of course, the shallow ditch can also be a ready-made sunny planting ditch. Lay a 3-5 cm thick first sand layer at the bottom of the planting ditch, and then insert the top of the catalpa cuttings into the first sand layer, which is now an upside-down insertion. Lay the thick second sand layer of 2-4 centimeters again in planting ditch, make the bottom of the catalpa cuttings that the second sand layer covers. It should be noted that the catalpa cuttings can be inserted individually or in bundles. The first sand layer and the second sand layer are not easy to be too thick or too thin, so as to ensure that the second sand layer has suitable ventilation ability, water loss prevention ability and heat preservation ability. In order to ensure that the sand layer has the above-mentioned suitable capabilities, generally the first sand layer and the second sand layer are wetted but impermeable (that is, the sand can reach the state of being held in a ball and falling apart when touched). The sand layer is the first culture substrate for catalpa cuttings.
第二砂层将楸树插穗的底端覆盖好后,再用塑料膜将楸树插穗的底端进行覆盖。即塑料膜再盖在第二砂层上面。塑料膜一般是指用聚氯乙烯、聚乙烯、聚丙烯、聚苯乙烯以及其他树脂制成的薄膜,用于包装,以及用作覆膜层。塑料膜可以为楸树插穗提供保温保湿的效果,加快楸树插穗的生根速率。半月以后,每隔2天检查一次楸树插穗底端的愈伤组织或新根发生情况。After the second sand layer has covered the bottom of the catalpa cuttings, the bottom of the catalpa cuttings is covered with a plastic film. That is, the plastic film is covered on the second sand layer. Plastic film generally refers to films made of polyvinyl chloride, polyethylene, polypropylene, polystyrene and other resins, used for packaging, and as a coating layer. The plastic film can provide heat preservation and moisturizing effect for the cuttings of catalpa, and accelerate the rooting rate of the cuttings of catalpa. After half a month, check the callus or new root occurrence at the bottom of the catalpa cuttings every 2 days.
待楸树插穗的底端的切口有愈伤组织形成或有根原基出现后,取出楸树插穗。After the cut at the bottom of the catalpa cuttings has callus formation or root primordia appear, take out the catalpa cuttings.
准备好第二培养基质,第二培养基质包括按重量份数计的以下原料:泥炭50-70份,珍珠岩20-30份。例如选用泥炭70份,珍珠岩30份。泥炭是一种经过几千年所形成的天然沼泽地产物(又称为草炭或是泥煤),是煤化程度最低的煤,同时也是煤最原始的状态。其无菌、无毒、无污染,通气性能好。泥炭质轻、持水、保肥、有利于微生物活动,增强生物性能,营养丰富,既是栽培基质,又是良好的土壤调解剂,并含有很高的有机质,腐殖酸及营养成份。但是泥炭属于不可再生资源,开采行为对环境破坏很大。加入一定的珍珠岩,一方面减少泥炭的用量,另一方面也能保证第二培养基质具有良好的孔隙度,使第二培养基质不仅具有充分的营养供给能力,还能保证第二培养基质具有良好的通风能力。第二培养基质也可以选用其他常见的基质,例如园土,培养土,山黄泥,兰花泥,砻糠灰,蛭石,河沙等,它们对环境要求是:渗水性好,有一定的保水能力,升温容易,保温良好。The second culture substrate is prepared, and the second culture substrate includes the following raw materials in parts by weight: 50-70 parts of peat, 20-30 parts of perlite. For example, 70 parts of peat and 30 parts of perlite are used. Peat is a natural swamp product (also known as peat or peat) formed after thousands of years. It is the coal with the lowest degree of coalification, and it is also the most primitive state of coal. It is sterile, non-toxic, non-polluting, and has good ventilation performance. Peat is light in weight, retains water and fertilizer, is beneficial to microbial activities, enhances biological performance, and is rich in nutrients. It is not only a cultivation substrate, but also a good soil conditioner, and contains high organic matter, humic acid and nutrients. However, peat is a non-renewable resource, and the mining behavior has caused great damage to the environment. Adding a certain amount of perlite, on the one hand, reduces the amount of peat, and on the other hand, ensures that the second culture substrate has good porosity, so that the second culture substrate not only has sufficient nutrient supply capacity, but also ensures that the second culture substrate has Good ventilation capacity. The second culture substrate can also use other common substrates, such as garden soil, culture soil, mountain yellow mud, orchid mud, chaff ash, vermiculite, river sand, etc. Their environmental requirements are: good water permeability, certain Water retention capacity, easy to heat up, good heat preservation.
楸树插穗插入第二培养基质时是将楸树插穗的底端(即有愈伤组织形成或有根原基出现的一端)插入第二培养基质内,此时即为正插。楸树插穗插入第二培养基质前,可以先对第二培养基质进行消毒,避免新生根出现大量坏死和楸树插穗大量腐烂的现象,提高楸树插穗的生根率和存活率。消毒的方式可以采用向第二培养基质喷洒高锰酸钾和/或多菌灵。When the catalpa cuttings are inserted into the second culture medium, the bottom end of the catalpa cuttings (that is, the end with callus formation or root primordium) is inserted into the second culture medium, which is positive insertion at this time. Before the cuttings of catalpa are inserted into the second culture medium, the second culture medium can be sterilized first, so as to avoid a large number of necrosis of new roots and a large amount of rot of the cuttings of catalpa, and improve the rooting rate and survival rate of the cuttings of catalpa. The way of disinfection can be to spray potassium permanganate and/or carbendazim to the second culture substrate.
为进一步提高楸树插穗在第二培养基质内的存活率,楸树插穗插入第二培养基质前,先将楸树插穗的底端蘸取激素后再插入消毒后的第二培养基质。激素包括生根粉,每一千克激素内包括1000-1500毫克的生根粉。激素的使用刺激了根系的生长,提高了楸树扦插生根成活率。激素内生根粉的含量不易过高或过低,否则对楸树插穗的生长的影响不明显或出现反作用。In order to further improve the survival rate of the catalpa cuttings in the second culture medium, before inserting the catalpa cuttings into the second culture medium, dip the bottom end of the catalpa cuttings into the hormone and then insert them into the sterilized second culture medium. The hormone includes root powder, and every kilogram of hormone includes 1000-1500 mg of root powder. The use of hormones stimulated the growth of roots and improved the rooting survival rate of catalpa cuttings. The content of the hormone endogenous rooting powder is not easy to be too high or too low, otherwise the influence on the growth of catalpa cuttings is not obvious or there will be adverse effects.
本发明实施例中,生根粉可以采用ABT生根粉。ABT生根粉是中国林科院王涛院士研制的一种新型的广谱高效系列绿色植物生长调节剂。它通过强化、调控植物内源激素的含量、重要酶的活性,促进生物分子的合成,诱导植物不定根或不定芽的形态建成,调节植物代谢作用强度,达到提高育苗造林成活率及林木生长量的目的。在林木、农作物、果树、花卉、物种经济和药用植物中广泛应用,均有很好的效果。In the embodiment of the present invention, the rooting powder can adopt ABT rooting powder. ABT rooting powder is a new broad-spectrum and high-efficiency series of green plant growth regulators developed by Academician Wang Tao of the Chinese Academy of Forestry. It strengthens and regulates the content of plant endogenous hormones and the activity of important enzymes, promotes the synthesis of biomolecules, induces the morphological establishment of plant adventitious roots or buds, regulates the intensity of plant metabolism, and achieves the goal of improving the survival rate of seedling afforestation and the growth of forest trees. Purpose. It is widely used in forest trees, crops, fruit trees, flowers, species economy and medicinal plants, all of which have good results.
激素最好在使用时进行现场配置,激素的药性能够得到较好的保持。It is best to configure the hormone on site when it is used, so that the drug properties of the hormone can be better maintained.
配置激素主要包括以下步骤:Configuring hormones mainly includes the following steps:
向生根粉添加酒精得到第一混合物,使第一混合物呈液体状;再向第一混合物中添加滑石粉得到第二混合物,使第二混合物呈糊状。再向烘干后的第二混合物添加滑石粉得到复合上述浓度要求的激素。在使用滑石粉时是使用酒精溶解代替水,不仅可以迅速挥发使激素变成粉末,而且可以使激素较长时间保留在根系,利于根系的吸收。楸树插穗的底端蘸取激素时,若楸树插穗的底端湿度不够,不能很好的蘸取激素,可以先将楸树插穗的底端蘸一下清水,再蘸取激素。Add alcohol to the root powder to obtain the first mixture, and make the first mixture in a liquid state; then add talcum powder to the first mixture to obtain the second mixture, and make the second mixture in a paste state. Then add talcum powder to the dried second mixture to obtain the hormone compounding the above-mentioned concentration requirements. When using talcum powder, it is dissolved in alcohol instead of water, which not only can quickly volatilize the hormone into powder, but also keep the hormone in the root system for a long time, which is conducive to the absorption of the root system. When dipping the bottom of the catalpa cuttings to pick up the hormone, if the bottom of the catalpa cuttings is not humid enough to pick up the hormone well, you can dip the bottom of the catalpa cuttings in clean water first, and then dip the hormone.
将楸树插穗的底端插入第二培养基质后,还包括:使楸树插穗的地上部分露出2-4个芽,其余的芽进行捆扎以阻止该部分芽的生长。可以用塑料膜对其余的芽进行捆扎。芽的捆扎减少了养分的消耗,使营养更集中在根系,对根的萌发起到了有利的作用,有利于提高扦插生活率。After inserting the bottom end of the catalpa cuttings into the second culture medium, it also includes: exposing 2-4 buds on the ground part of the catalpa cuttings, and binding the remaining buds to prevent the growth of this part of buds. The rest of the shoots can be bundled with plastic wrap. The bundling of buds reduces the consumption of nutrients and makes the nutrients more concentrated in the root system, which has a favorable effect on the germination of roots and is conducive to improving the survival rate of cuttings.
楸树插穗在第二培养基质内的培养条件一般是,温度25℃左右,湿度85-90%。每隔一周喷施多菌灵和/或高锰酸钾,喷施后达到楸树插穗叶片发白的效果即可。在生长前期多进行浇水少量多次,在生长的后期(愈伤组织大量形成后)进行少浇水,两到三天浇一次,30天以后进行中耕除草。The culture conditions of catalpa cuttings in the second culture medium are generally about 25°C in temperature and 85-90% in humidity. Spray carbendazim and/or potassium permanganate every other week, and after spraying, the effect of turning the leaves of catalpa cuttings can be achieved. Carry out a small amount of watering many times in the early stage of growth, carry out less watering in the late stage of growth (after callus is formed in a large number), water once every two to three days, carry out intertillage and weeding after 30 days.
需要说明的是,上述楸树扦插方法也适用于其他植株的扦插,例如桃树、柚子树、松树等等。It should be noted that the above-mentioned catalpa cutting method is also applicable to cuttings of other plants, such as peach trees, grapefruit trees, pine trees and so on.
以下结合实施例对本发明的特征和性能作进一步的详细描述。The characteristics and performance of the present invention will be described in further detail below in conjunction with the examples.
实施例1Example 1
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗(后续实施例中也均选用硬枝)。选用向阳的种植沟。种植沟内填充泥土作为第一培养基质。将楸树插穗的顶端插入第一培养基质,待楸树插穗的底端的切口有愈伤组织形成或有根原基出现,再取出楸树插穗。取出楸树插穗后,再将楸树插穗的底端插入第二培养基质内进行后续培育。第二培养基质由80g泥炭和40g珍珠岩组成。Get ready catalpa cuttings (also all select hard branch in subsequent embodiment). Choose sunny planting ditch. Fill the planting ditch with soil as the first culture substrate. Insert the top of the catalpa cuttings into the first culture substrate, and take out the catalpa cuttings after callus formation or root primordia appear at the incision at the bottom of the catalpa cuttings. After taking out the catalpa cuttings, insert the bottom end of the catalpa cuttings into the second culture medium for subsequent cultivation. The second culture substrate consisted of 80 g of peat and 40 g of perlite.
实施例2Example 2
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。Prepare the catalpa cuttings.
选用向阳的种植沟。种植沟内填充泥土作为第一培养基质。将楸树插穗的顶端插入第一培养基质,再用塑料膜将楸树插穗的底端进行覆盖。待楸树插穗的底端的切口有愈伤组织形成或有根原基出现后,取出楸树插穗。取出楸树插穗后,再将楸树插穗的底端插入第二培养基质内进行后续培育。第二培养基质由80g培养土和30g蛭石组成。Choose sunny planting ditch. Fill the planting ditch with soil as the first culture substrate. Insert the top of the catalpa cuttings into the first culture substrate, and then cover the bottom of the catalpa cuttings with a plastic film. After the cut at the bottom of the catalpa cuttings has callus formation or root primordia appear, take out the catalpa cuttings. After taking out the catalpa cuttings, insert the bottom end of the catalpa cuttings into the second culture medium for subsequent cultivation. The second culture substrate is composed of 80g culture soil and 30g vermiculite.
实施例3Example 3
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。Prepare the catalpa cuttings.
选用向阳的种植沟。在种植沟的底部铺设4厘米厚的第一砂层,再将楸树插穗的顶端插入第一砂层,此时即为倒插。再在种植沟内铺设3厘米厚的第二砂层,使第二砂层覆盖楸树插穗的底端。将第一砂层、第二砂层浇湿但不透(即砂可以达到手握成团,一触即散的状态)。砂层即作为楸树插穗的第一培养基质。第二砂层将楸树插穗的底端覆盖好后,再用塑料膜将楸树插穗的底端进行覆盖。即塑料膜再盖在第二砂层上面。半月以后,每隔2天检查一次楸树插穗底端的愈伤组织或新根发生情况。Choose sunny planting ditch. Lay a 4 cm thick first sand layer at the bottom of the planting ditch, and then insert the top of the catalpa cuttings into the first sand layer, which is now reverse insertion. Lay the second sand layer of 3 centimeters thick in planting ditch again, make the bottom of second sand layer cover the catalpa tree cuttings. Wet the first sand layer and the second sand layer but impermeable (that is, the sand can reach the state of being held in a ball and falling apart when touched). The sand layer is the first culture substrate for catalpa cuttings. After the second sand layer has covered the bottom of the catalpa cuttings, the bottom of the catalpa cuttings is covered with a plastic film. That is, the plastic film is covered on the second sand layer. After half a month, check the callus or new root occurrence at the bottom of the catalpa cuttings every 2 days.
待楸树插穗的底端的切口有愈伤组织形成或有根原基出现后,取出楸树插穗。取出楸树插穗后,再将楸树插穗的底端插入第二培养基质内进行后续培育。第二培养基质由80g培养土和30g砻糠灰组成。After the cut at the bottom of the catalpa cuttings has callus formation or root primordia appear, take out the catalpa cuttings. After taking out the catalpa cuttings, insert the bottom end of the catalpa cuttings into the second culture medium for subsequent cultivation. The second culture substrate is composed of 80g of culture soil and 30g of husk chaff ash.
实施例4Example 4
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。选用向阳的种植沟。种植沟内填充泥土作为第一培养基质。将楸树插穗的顶端插入第一培养基质,待楸树插穗的底端的切口有愈伤组织形成或有根原基出现,再取出楸树插穗。取出楸树插穗后,再将楸树插穗的底端插入第二培养基质内进行后续培育。第二培养基质由70g泥炭和30g珍珠岩组成。Prepare the catalpa cuttings. Choose sunny planting ditch. Fill the planting ditch with soil as the first culture substrate. Insert the top of the catalpa cuttings into the first culture substrate, and take out the catalpa cuttings after callus formation or root primordia appear at the incision at the bottom of the catalpa cuttings. After taking out the catalpa cuttings, insert the bottom end of the catalpa cuttings into the second culture medium for subsequent cultivation. The second culture substrate consisted of 70 g of peat and 30 g of perlite.
实施例5Example 5
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。选用向阳的种植沟。种植沟内填充泥土作为第一培养基质。将楸树插穗的顶端插入第一培养基质,待楸树插穗的底端的切口有愈伤组织形成或有根原基出现,再取出楸树插穗。取出楸树插穗后,再将楸树插穗的底端插入经过消毒的第二培养基质内进行后续培育。第二培养基质由70g泥炭和30g珍珠岩组成。采用多菌灵对第二培养基质进行消毒。Prepare the catalpa cuttings. Choose sunny planting ditch. Fill the planting ditch with soil as the first culture substrate. Insert the top of the catalpa cuttings into the first culture substrate, and take out the catalpa cuttings after callus formation or root primordia appear at the incision at the bottom of the catalpa cuttings. After taking out the catalpa cuttings, insert the bottom end of the catalpa cuttings into the sterilized second culture medium for subsequent cultivation. The second culture substrate consisted of 70 g of peat and 30 g of perlite. The second culture substrate was sterilized with carbendazim.
实施例6Example 6
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。选用向阳的种植沟。在种植沟的底部铺设4厘米厚的第一砂层,再将楸树插穗的顶端插入第一砂层,此时即为倒插。再在种植沟内铺设3厘米厚的第二砂层,使第二砂层覆盖楸树插穗的底端。将第一砂层、第二砂层浇湿但不透(即砂可以达到手握成团,一触即散的状态)。砂层即作为楸树插穗的第一培养基质。Prepare the catalpa cuttings. Choose sunny planting ditch. Lay a 4 cm thick first sand layer at the bottom of the planting ditch, and then insert the top of the catalpa cuttings into the first sand layer, which is now reverse insertion. Lay the second sand layer of 3 centimeters thick in planting ditch again, make the bottom of second sand layer cover the catalpa tree cuttings. Wet the first sand layer and the second sand layer but impermeable (that is, the sand can reach the state of being held in a ball and falling apart when touched). The sand layer is the first culture substrate for catalpa cuttings.
第二砂层将楸树插穗的底端覆盖好后,再用塑料膜将楸树插穗的底端进行覆盖。即塑料膜再盖在第二砂层上面。待楸树插穗的底端的切口有愈伤组织形成或有根原基出现,再取出楸树插穗。After the second sand layer has covered the bottom of the catalpa cuttings, the bottom of the catalpa cuttings is covered with a plastic film. That is, the plastic film is covered on the second sand layer. Wait for callus to form or root primordia to appear at the incision at the bottom of the catalpa cuttings, then take out the catalpa cuttings.
取出楸树插穗后,再将楸树插穗的底端插入经过消毒的第二培养基质内进行后续培育。第二培养基质由70g泥炭和30g珍珠岩组成。采用多菌灵对第二培养基质进行消毒。其中,楸树插穗的底端上有愈伤组织形成或有根原基出现的部位蘸取激素后再插入第二培养基质。激素中的生根粉浓度为1400mg/kg。After taking out the catalpa cuttings, insert the bottom end of the catalpa cuttings into the sterilized second culture medium for subsequent cultivation. The second culture substrate consisted of 70 g of peat and 30 g of perlite. The second culture substrate was sterilized with carbendazim. Wherein, the bottom of the catalpa cuttings has callus formation or root primordia are dipped in the hormone and then inserted into the second culture medium. The root powder concentration in the hormone is 1400mg/kg.
实施例7Example 7
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。Prepare the catalpa cuttings.
在圃地向阳的地方挖一浅沟,沟深15cm。在种植沟的底部铺设4厘米厚的第一砂层,再将楸树插穗的顶端插入第一砂层,此时即为倒插。再在种植沟内铺设3厘米厚的第二砂层,使第二砂层覆盖楸树插穗的底端。将第一砂层、第二砂层浇湿但不透(即砂可以达到手握成团,一触即散的状态)。砂层即作为楸树插穗的第一培养基质。Dig a shallow ditch with a depth of 15 cm in the sunny place of the nursery. Lay a 4 cm thick first sand layer at the bottom of the planting ditch, and then insert the top of the catalpa cuttings into the first sand layer, which is now reverse insertion. Lay the second sand layer of 3 centimeters thick in planting ditch again, make the bottom of second sand layer cover the catalpa tree cuttings. Wet the first sand layer and the second sand layer but impermeable (that is, the sand can reach the state of being held in a ball and falling apart when touched). The sand layer is the first culture substrate for catalpa cuttings.
第二砂层将楸树插穗的底端覆盖好后,再用塑料膜将楸树插穗的底端进行覆盖。即塑料膜再盖在第二砂层上面。半月以后,每隔3天检查一次楸树插穗底端的愈伤组织或新根发生情况。After the second sand layer has covered the bottom of the catalpa cuttings, the bottom of the catalpa cuttings is covered with a plastic film. That is, the plastic film is covered on the second sand layer. After half a month, check the callus or new root occurrence at the bottom of the catalpa cuttings every 3 days.
待楸树插穗的底端的切口有愈伤组织形成或有根原基出现后,取出楸树插穗。After the cut at the bottom of the catalpa cuttings has callus formation or root primordia appear, take out the catalpa cuttings.
准备好第二培养基质,第二培养基质包括:泥炭60g,珍珠岩25g。Prepare the second culture substrate, which includes: 60g of peat and 25g of perlite.
楸树插穗插入第二培养基质时是将楸树插穗的底端(即有愈伤组织形成或有根原基出现的一端)插入第二培养基质内,此时即为正插。楸树插穗插入第二培养基质前,向第二培养基质喷洒高锰酸钾和多菌灵进行消毒。When the catalpa cuttings are inserted into the second culture medium, the bottom end of the catalpa cuttings (that is, the end with callus formation or root primordium) is inserted into the second culture medium, which is positive insertion at this time. Before inserting the catalpa cuttings into the second culture medium, spray potassium permanganate and carbendazim to the second culture medium for disinfection.
楸树插穗插入第二培养基质前,先将楸树插穗的底端蘸取激素后再插入消毒后的第二培养基质。激素包括ABT生根粉,每一千克激素内包括1300毫克的ABT生根粉。激素在使用时进行现场配置。Before inserting the catalpa cuttings into the second culture medium, dip the bottom end of the catalpa cuttings into the hormone and then insert the sterilized second culture medium. The hormones include ABT rooting powder, and each kilogram of hormones includes 1300 mg of ABT rooting powder. Hormones are configured on-site at the time of use.
配置激素步骤如下:向生根粉添加酒精得到第一混合物,使第一混合物呈液体状;再向第一混合物中添加滑石粉得到第二混合物,使第二混合物呈糊状。再向烘干后的第二混合物添加滑石粉得到复合上述浓度要求的激素。即该激素由滑石粉与生根粉混合而成。The steps of configuring the hormone are as follows: adding alcohol to the rooting powder to obtain the first mixture, making the first mixture in a liquid state; adding talcum powder to the first mixture to obtain the second mixture, making the second mixture in a paste state. Then add talcum powder to the dried second mixture to obtain the hormone compounding the above-mentioned concentration requirements. That is, the hormone is mixed with talcum powder and root powder.
将楸树插穗的底端插入消毒后的第二培养基质后,还包括:使楸树插穗的地上部分露出2个芽,其余的芽进行捆扎以阻止该部分芽的生长。After inserting the bottom end of the catalpa cuttings into the sterilized second culture medium, it also includes: exposing two buds on the ground part of the catalpa cuttings, and binding the remaining buds to prevent the growth of this part of buds.
楸树插穗在第二培养基质内的培养条件为,温度26℃,湿度88%。每隔二周喷施多菌灵,喷施后达到楸树插穗叶片发白的效果即可。在生长前期多进行浇水少量多次,在生长的后期(愈伤组织大量形成后)进行少浇水,两到三天浇一次,45天以后进行中耕除草。The culture conditions of the catalpa cuttings in the second culture medium are temperature of 26° C. and humidity of 88%. Spray carbendazim every two weeks, and after spraying, the effect of turning the leaves of the catalpa cuttings can be achieved. Carry out a small amount of watering many times in the early stage of growth, carry out less watering in the late stage of growth (after callus is formed in a large number), water once every two to three days, carry out intertillage and weeding after 45 days.
实施例8Example 8
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。Prepare the catalpa cuttings.
在圃地向阳的地方挖一浅沟,沟深18cm。在种植沟的底部铺设5厘米厚的第一砂层,再将楸树插穗的顶端插入第一砂层,此时即为倒插。再在种植沟内铺设4厘米厚的第二砂层,使第二砂层覆盖楸树插穗的底端。将第一砂层、第二砂层浇湿但不透(即砂可以达到手握成团,一触即散的状态)。砂层即作为楸树插穗的第一培养基质。Dig a shallow ditch with a depth of 18 cm in the sunny place of the nursery. Lay a 5 cm thick first sand layer at the bottom of the planting ditch, and then insert the top of the catalpa cuttings into the first sand layer, which is now reverse insertion. Lay the second sand layer of 4 centimeters thick in planting ditch again, make the bottom of second sand layer cover the catalpa cutting. Wet the first sand layer and the second sand layer but impermeable (that is, the sand can reach the state of being held in a ball and falling apart when touched). The sand layer is the first culture substrate for catalpa cuttings.
第二砂层将楸树插穗的底端覆盖好后,再用塑料膜将楸树插穗的底端进行覆盖。即塑料膜再盖在第二砂层上面。半月以后,每隔2天检查一次楸树插穗底端的愈伤组织或新根发生情况。After the second sand layer has covered the bottom of the catalpa cuttings, the bottom of the catalpa cuttings is covered with a plastic film. That is, the plastic film is covered on the second sand layer. After half a month, check the callus or new root occurrence at the bottom of the catalpa cuttings every 2 days.
待楸树插穗的底端的切口有愈伤组织形成或有根原基出现后,取出楸树插穗。After the cut at the bottom of the catalpa cuttings has callus formation or root primordia appear, take out the catalpa cuttings.
准备好第二培养基质,第二培养基质包括:泥炭50g,珍珠岩20g。Prepare the second culture substrate, which includes: 50g of peat and 20g of perlite.
楸树插穗插入第二培养基质时是将楸树插穗的底端(即有愈伤组织形成或有根原基出现的一端)插入第二培养基质内,此时即为正插。楸树插穗插入第二培养基质前,向第二培养基质喷洒高锰酸钾进行消毒。When the catalpa cuttings are inserted into the second culture medium, the bottom end of the catalpa cuttings (that is, the end with callus formation or root primordium) is inserted into the second culture medium, which is positive insertion at this time. Before inserting the catalpa cuttings into the second culture substrate, spray potassium permanganate on the second culture substrate for disinfection.
楸树插穗插入第二培养基质前,先将楸树插穗的底端蘸取激素后再插入消毒后的第二培养基质。激素包括ABT生根粉,每一千克激素内包括1500毫克的ABT生根粉。激素在使用时进行现场配置。Before inserting the catalpa cuttings into the second culture medium, dip the bottom end of the catalpa cuttings into the hormone and then insert the sterilized second culture medium. The hormones include ABT rooting powder, and each kilogram of hormones includes 1500 mg of ABT rooting powder. Hormones are configured on-site at the time of use.
配置激素步骤如下:向生根粉添加酒精得到第一混合物,使第一混合物呈液体状;再向第一混合物中添加滑石粉得到第二混合物,使第二混合物呈糊状。再向烘干后的第二混合物添加滑石粉得到复合上述浓度要求的激素。The steps of configuring the hormone are as follows: adding alcohol to the rooting powder to obtain the first mixture, making the first mixture in a liquid state; adding talcum powder to the first mixture to obtain the second mixture, making the second mixture in a paste state. Then add talcum powder to the dried second mixture to obtain the hormone compounding the above-mentioned concentration requirements.
将楸树插穗的底端插入消毒后的第二培养基质后,还包括:使楸树插穗的地上部分露出2个芽,其余的芽进行捆扎以阻止该部分芽的生长。After inserting the bottom end of the catalpa cuttings into the sterilized second culture medium, it also includes: exposing two buds on the ground part of the catalpa cuttings, and binding the remaining buds to prevent the growth of this part of buds.
楸树插穗在第二培养基质内的培养条件为,温度24℃,湿度90%。每隔一周喷施多菌灵,喷施后达到楸树插穗叶片发白的效果即可。在生长前期多进行浇水少量多次,在生长的后期(愈伤组织大量形成后)进行少浇水,两到三天浇一次,30天以后进行中耕除草。The culture conditions of the catalpa cuttings in the second culture medium are temperature of 24°C and humidity of 90%. Spray carbendazim every other week, and after spraying, the effect of turning the leaves of the catalpa cuttings can be achieved. Carry out a small amount of watering many times in the early stage of growth, carry out less watering in the late stage of growth (after callus is formed in a large number), water once every two to three days, carry out intertillage and weeding after 30 days.
实施例9Example 9
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗。Prepare the catalpa cuttings.
在圃地向阳的地方挖一浅沟,沟深18cm。在种植沟的底部铺设3厘米厚的第一砂层,再将楸树插穗的顶端插入第一砂层,此时即为倒插。再在种植沟内铺设2厘米厚的第二砂层,使第二砂层覆盖楸树插穗的底端。将第一砂层、第二砂层浇湿但不透(即砂可以达到手握成团,一触即散的状态)。砂层即作为楸树插穗的第一培养基质。Dig a shallow ditch with a depth of 18 cm in the sunny place of the nursery. Lay a 3 cm thick first sand layer at the bottom of the planting ditch, and then insert the top of the catalpa cuttings into the first sand layer, which is now reverse insertion. Lay the second sand layer of 2 centimeters thick in planting ditch again, make the bottom of second sand layer cover the catalpa cuttings. Wet the first sand layer and the second sand layer but impermeable (that is, the sand can reach the state of being held in a ball and falling apart when touched). The sand layer is the first culture substrate for catalpa cuttings.
第二砂层将楸树插穗的底端覆盖好后,再用塑料膜将楸树插穗的底端进行覆盖。即塑料膜再盖在第二砂层上面。半月以后,每隔2天检查一次楸树插穗底端的愈伤组织或新根发生情况。After the second sand layer has covered the bottom of the catalpa cuttings, the bottom of the catalpa cuttings is covered with a plastic film. That is, the plastic film is covered on the second sand layer. After half a month, check the callus or new root occurrence at the bottom of the catalpa cuttings every 2 days.
待楸树插穗的底端的切口有愈伤组织形成或有根原基出现后,取出楸树插穗。After the cut at the bottom of the catalpa cuttings has callus formation or root primordia appear, take out the catalpa cuttings.
准备好第二培养基质,第二培养基质包括:泥炭70g,珍珠岩30g。Prepare the second culture substrate, which includes: 70g of peat and 30g of perlite.
楸树插穗插入第二培养基质时是将楸树插穗的底端(即有愈伤组织形成或有根原基出现的一端)插入第二培养基质内,此时即为正插。楸树插穗插入第二培养基质前,向第二培养基质喷洒多菌灵进行消毒。When the catalpa cuttings are inserted into the second culture medium, the bottom end of the catalpa cuttings (that is, the end with callus formation or root primordium) is inserted into the second culture medium, which is positive insertion at this time. Before inserting the catalpa cuttings into the second culture medium, spray carbendazim to the second culture medium for disinfection.
楸树插穗插入第二培养基质前,先将楸树插穗的底端蘸取激素后再插入消毒后的第二培养基质。激素包括ABT生根粉,每一千克激素内包括1000毫克的ABT生根粉。激素在使用时进行现场配置。Before inserting the catalpa cuttings into the second culture medium, dip the bottom end of the catalpa cuttings into the hormone and then insert the sterilized second culture medium. Hormones include ABT rooting powder, and each kilogram of hormones includes 1000 mg of ABT rooting powder. Hormones are configured on-site at the time of use.
配置激素步骤如下:向生根粉添加酒精得到第一混合物,使第一混合物呈液体状;再向第一混合物中添加滑石粉得到第二混合物,使第二混合物呈糊状。再向烘干后的第二混合物添加滑石粉得到复合上述浓度要求的激素。The steps of configuring the hormone are as follows: adding alcohol to the rooting powder to obtain the first mixture, making the first mixture in a liquid state; adding talcum powder to the first mixture to obtain the second mixture, making the second mixture in a paste state. Then add talcum powder to the dried second mixture to obtain the hormone compounding the above-mentioned concentration requirements.
将楸树插穗的底端插入消毒后的第二培养基质后,还包括:使楸树插穗的地上部分露出3个芽,其余的芽进行捆扎以阻止该部分芽的生长。After inserting the bottom end of the catalpa cuttings into the sterilized second culture medium, it also includes: exposing 3 buds on the ground part of the catalpa cuttings, and binding the rest of the buds to prevent the growth of this part of the buds.
楸树插穗在第二培养基质内的培养条件为,温度25℃,湿度85%。每隔一周喷施多菌灵,喷施后达到楸树插穗叶片发白的效果即可。在生长前期多进行浇水少量多次,在生长的后期(愈伤组织大量形成后)进行少浇水,两到三天浇一次,30天以后进行中耕除草。The culture conditions of the catalpa cuttings in the second culture medium are temperature of 25° C. and humidity of 85%. Spray carbendazim every other week, and after spraying, the effect of turning the leaves of the catalpa cuttings can be achieved. Carry out a small amount of watering many times in the early stage of growth, carry out less watering in the late stage of growth (after callus is formed in a large number), water once every two to three days, carry out intertillage and weeding after 30 days.
对比例comparative example
本实施例提供一种楸树苗,其采用以下方法培育而成。The present embodiment provides a catalpa sapling, which is cultivated by the following method.
准备好楸树插穗(硬枝)。Prepare catalpa cuttings (hard branches).
选定向阳种植沟。在种植沟的底部铺设基质(由泥炭70g和珍珠岩30g组成),再将楸树插穗正插于基质中。楸树插穗插入基质前,先将楸树插穗的底端蘸取激素后再插入基质。激素为ABT生根粉与水的混合溶液,ABT生根粉浓度为1000mg/kg。Select the sunny planting ditch. Lay substrate (composed of 70g of peat and 30g of perlite) at the bottom of the planting ditch, and insert the catalpa cuttings in the substrate. Before inserting the catalpa cuttings into the matrix, dip the bottom of the catalpa cuttings into the hormone and then insert into the matrix. The hormone is a mixed solution of ABT rooting powder and water, and the concentration of ABT rooting powder is 1000 mg/kg.
楸树插穗在基质内的培养条件为,温度25℃,湿度85%。每隔一周喷施多菌灵对基质和楸树插穗进行消毒,喷施后达到楸树插穗叶片发白的效果即可。在生长前期多进行浇水少量多次,在生长的后期(愈伤组织大量形成后)进行少浇水,两到三天浇一次,30天以后进行中耕除草。The cultivation conditions of the catalpa cuttings in the substrate are temperature 25°C and humidity 85%. Spray carbendazim every other week to disinfect the substrate and the cuttings of the catalpa, and after spraying, the effect of turning the leaves of the cuttings of the catalpa can be achieved. Carry out a small amount of watering many times in the early stage of growth, carry out less watering in the late stage of growth (after callus is formed in a large number), water once every two to three days, carry out intertillage and weeding after 30 days.
试验例Test case
采用实施例7-9提供的三种培育方法分别对200株楸树插穗(均为硬枝)进行培育。150天后对三种培育方法培育的植株分别进行成活情况统计。Adopt three kinds of breeding methods that embodiment 7-9 provides to cultivate respectively 200 strains of Chinese catalpa cuttings (all are hard branches). After 150 days, statistics were made on the survival of the plants cultivated by the three cultivation methods.
采用对比例提供的培育方法对200株楸树插穗(均为硬枝)进行培育。150天后对200株植株进行成活情况统计。Adopt the cultivation method that comparative example provides to cultivate 200 catalpa tree cuttings (all are hard branches). After 150 days, the survival statistics of 200 plants were carried out.
四种培育方法培育的植株成活情况如表1所示。The survival conditions of the plants cultivated by the four cultivation methods are shown in Table 1.
表1植株成活情况统计Table 1 Plant Survival Statistics
由表1的统计结果可知,利用本发明实施例提供的楸树扦插方法培育的楸树苗,其培育成活率明显高于利用对比例提供的传统的楸树扦插方法的培育成活率。As can be seen from the statistical results of Table 1, the catalpa saplings cultivated by the catalpa cuttage method provided by the embodiments of the present invention have a significantly higher survival rate than that of the traditional catalpa cuttage method provided by comparative examples.
以上仅为本发明的优选实施例而已,并不用于限制本发明,对于本领域的技术人员来说,本发明可以有各种更改和变化。凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.
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