CN108982832A - A kind of immunodiagnosis detection device - Google Patents

Abstract

The invention discloses a kind of immunodiagnosis detection devices, belong to technical field of pharmaceutical biotechnology.Immunodiagnosis detection device of the present invention includes matrix and water-holding portion, and protein matter is coated on matrix, and absorbent section is below matrix, and water-holding portion is for absorbing the liquid permeated down from matrix.Detection device provided by the invention, the water-holding portion being located at below matrix is set, water-holding portion can absorb the excess reagent being added in the operation such as cleaning in the detection process, and cleaning solution need to only be added dropwise on matrix when carrying out and the operation such as cleaning, structure is simple, the complex operations for being added, extracting out cleaning solution repeatedly are eliminated, operation is easier, more save the time, detection efficiency is higher and pollution risk is small, testing result is more reliable.

Description

A detection device for immunodiagnosis

technical field

The invention relates to the technical field of medicine and biology, in particular to a detection device for immunodiagnosis.

Background technique

At present, the immunodiagnostic devices on the market need to perform multiple cleaning actions during the detection process. The process is extremely cumbersome. Improper cleaning operations will have a great impact on the test results. Repeated aspiration and addition processes may also increase the risk of contamination.

In addition, in the existing immunodiagnostic devices, the solid phases used for coating antibodies or antigens are microtiter plates, magnetic beads, membrane strips, etc., and the production process is relatively complicated; the solid phases on the market are detected after the reaction. The design of the detector detection stage is also unreasonable, and the precision of the detector detection is uneven; the existing reaction time is very long, which increases the time for the output of the detection results; the general solid phase has been coated with a specific antigen or Protein substances such as antibodies have poor versatility.

Contents of the invention

The object of the present invention is to provide a detection device for immunodiagnosis which is easy to operate.

For reaching this purpose, the present invention adopts following technical scheme:

A detection device for immunodiagnosis, comprising:

a substrate coated with a proteinaceous substance; and

A water-absorbing part, which is located under the substrate, is used for absorbing the liquid penetrating down from the substrate.

Further, the base body is made of porous filter material.

Further, it also includes a reaction container, the substrate and the water absorbing part are arranged in the reaction container, the reaction container has an opening, and the opening is located at the top of the reaction container and directly above the substrate.

Further, the opening is funnel-shaped.

Further, the contour of the inner wall surface of the opening matches the contour of the outer wall surface of the detection head of the detector.

Further, the bottom of the reaction container is provided with openings.

Further, the reaction container includes a body and a cover detachably arranged on the body, and the opening is arranged on the top of the cover.

Further, a positioning part is provided outside the body of the reaction container.

Further, the positioning part is in the shape of a ring.

Further, the substrate is coated with anti-biotin antibody or streptavidin, which is used for specific binding to the biotinylated protein added to the substrate.

The beneficial effects of the present invention are as follows:

The detection device provided by the present invention is provided with a water absorbing part located below the substrate, which has a simple structure, and the water absorbing part can absorb the excess reagent dripped in during the cleaning and other operations during the detection process, and only needs to be placed on the substrate during cleaning and other operations. Just add the cleaning solution dropwise, eliminating the complicated operation of repeatedly adding and withdrawing the cleaning solution. The operation is simpler, more time-saving, the detection efficiency is higher, the risk of contamination is small, and the detection results are more reliable.

Description of drawings

Fig. 1 is an axonometric view of a detection device for immunodiagnosis provided by a specific embodiment of the present invention;

Fig. 2 is an exploded view of the detection device for immunodiagnosis provided by a specific embodiment of the present invention;

Fig. 3 is a bottom view of the detection device for immunodiagnosis provided by a specific embodiment of the present invention;

Fig. 4 is a top view of a detection device for immunodiagnosis provided by a specific embodiment of the present invention;

Fig. 5 is a front view of a detection device for immunodiagnosis provided by a specific embodiment of the present invention;

Fig. 6 is a side view of the detection device for immunodiagnosis provided by a specific embodiment of the present invention.

Reference signs:

10. Reaction container; 20. Substrate; 30. Water absorption part; 40. Positioning part; 11. Body; 12. Cover; 111. Open hole; 121. Opening;

Detailed ways

The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and through specific implementation methods.

As shown in FIG. 1 and FIG. 2 , the specific embodiment of the present invention provides a detection device for immunodiagnosis, which includes a reaction container 10 , and a base 20 and a water-absorbing part 30 disposed in the reaction container 10 . The reaction vessel 10 is provided with an opening 121. The base 20 is preferably coated with anti-biotin antibody, but not limited thereto. The water absorbing part 30 is arranged below the base 20 and is made of a water absorbing material. The water absorbing part 30 is used to absorb water from the base 20. The penetrating liquid, for example, the water-absorbing part 30 absorbs the cleaning liquid added during the detection process. The opening 121 is preferably located directly above the base 20 , and the base 20 is located directly above the water-absorbing portion 30 . Wherein, the reaction vessel 10 is preferably but not limited to be made of plastic materials such as polyethylene and polypropylene. The water absorbing portion 30 is preferably but not limited to be cylindrical. The water-absorbing material is preferably but not limited to acetyl fiber, and a paper wrapping layer can be provided on the outer surface of the acetyl fiber. The reaction vessel 10 is preferably but not limited to include a body 11 and a cover 12 detachably disposed on the body 11 , and the opening 121 is disposed on the top of the cover 12 . The cover body 12 can be engaged with the upper end of the main body 11 through a snap ring 122 fixed on the bottom thereof. In this specific embodiment, the snap ring 122 is clamped on the upper inner periphery of the body 11 . In other embodiments, the snap ring 122 can also be snapped on the upper outer periphery of the main body 11 . It should be noted that, in other embodiments, any protein antibody or antigen such as streptavidin may also be coated on the substrate 20 . It should be noted that in other specific embodiments, the reaction vessel 10 may not be provided.

The detection device provided in this specific embodiment is provided with a water absorption part 30 located under the base body 20, which has a simple structure and low production cost, and the water absorption part 30 can absorb the excess reagent added in the cleaning and other operations during the detection process, and after cleaning During the operation, it is only necessary to add the cleaning solution onto the substrate 20, eliminating the complicated operation of repeatedly pumping liquid, making the operation easier, saving time, higher detection efficiency, less risk of contamination, and more reliable detection results.

In order to further facilitate the excess reagent to permeate from the base body 20 to the water-absorbing portion 30 and prevent the excess reagent from overflowing to the periphery, the base body 20 is also preferably made of a porous filter material. For example, the matrix 20 is a porous filter membrane made of porous materials such as glass fibers. Preferably, the outer wall of the water absorbing portion 30 is substantially aligned with the outer wall of the base body 20 or the outer wall of the water absorbing portion 30 protrudes from the outer wall of the base body 20 . Because the reaction container uses a porous filter membrane as a solid phase substrate, the protein substances coated on it can immobilize different types of antigens or antibodies through biotin, so that different antigens or antibodies can be detected using the same solid phase, and the versatility Good; at the same time, the biotinylated antigen/antibody is in the liquid phase during preparation, and the reaction between the liquid phase and the liquid phase is carried out between the substance to be tested (serum, etc.) and the biotinylated antigen/antibody, and the reaction speed is relatively fast. Then it is combined with a solid phase to prepare a detection object, and then an enzyme marker is added for incubation. After the cleaning solution is added dropwise, a chromogenic solution is added for a color reaction. The overall reaction speed is fast and the efficiency is high.

As shown in FIG. 3 , an opening 111 is provided at the bottom of the reaction vessel 10 . Specifically, an opening 111 is provided at the bottom of the body 11 . The opening 111 is preferably disposed directly under the water absorbing portion 30 . Normally, the water absorbing part 30 can absorb excess reagent, but an opening 111 is provided at the bottom of the body 11 to allow the reagent to be discharged from the opening 111 at the bottom when the amount of reagent is too large. The number and distribution of openings 111 can be set according to specific discharge requirements. In this specific embodiment, the number of openings 121 is three, and they are arranged in an isosceles triangle, more preferably, in an isosceles triangle.

As shown in FIG. 1 , FIG. 2 and FIG. 4 , in order to facilitate the entry of reagents into the reaction vessel 10 and prevent outward splashing, the opening 121 is located at the top of the reaction vessel 10 , and the opening 121 is funnel-shaped. In order to facilitate detection of the reaction results with the detector such as a photometric instrument, the detection head of the detector is usually well matched in the shape of a truncated cone, so as to improve the detection accuracy. contours match.

In this specific embodiment, the substrate 20 is coated with an anti-biotin antibody, which is used to specifically bind to biotinylated proteins, and the non-specific adsorption on the substrate 20 is very low, ensuring high sensitivity and high specificity of detection . In order to facilitate positioning during the detection process, as shown in FIG. 1 , FIG. 5 and FIG. 6 , a positioning portion 40 is provided outside the main body 11 . The structural size of the positioning part 40 is consistent with the structure and size of the fixed position in the detection environment. In this specific embodiment, the positioning part 40 is in the shape of a ring, and is preferably a rectangular ring-shaped plate.

The method of using the detection device for immunodiagnosis in this specific embodiment is as follows:

First use buffer to wet the reaction vessel 10, then add the mixture of the pre-reacted sample and biotinylation reagent (37°C for 6 minutes) onto the substrate 20 of the reaction vessel 10, react at 37°C for 2 minutes, then add the enzyme label After reacting at 37°C for 2 minutes, adding the cleaning solution twice, the photometer was tightly fitted to the opening 121 of the reaction vessel 10 to block the interference of natural light, and the photometry was performed several times to obtain the photometry data.

Buffers, biotinylated reagents, and enzyme labels can be determined according to the specific substances to be detected. For example, when the syphilis antibody in the serum is to be detected, a biotinylated syphilis antigen can be prepared, and the serum and the biotinylated syphilis antigen are first mixed (both in liquid phase) to react in the specific detection, since both are The reaction time in the liquid phase is relatively fast, and then the mixed solution after the reaction is added into the reaction vessel 10 , and it will bind with the anti-biotin antibody on the substrate 20 in the reaction vessel 10 to immobilize the reaction substance.

The above describes the technical principles of the present invention in conjunction with specific embodiments. These descriptions are only for explaining the principles of the present invention, and cannot be construed as limiting the protection scope of the present invention in any way. Based on the explanations herein, those skilled in the art can think of other specific implementation modes of the present invention without creative efforts, and these modes will all fall within the protection scope of the present invention.

Claims (10)

1. A detection device for immunodiagnosis, comprising: a substrate (20) coated with a proteinaceous substance; and The water absorbing part (30) is located under the base body (20), and the water absorbing part (30) is used for absorbing liquid penetrating down from the base body (20). 2. The detection device for immunodiagnosis according to claim 1, characterized in that, the base (20) is made of porous filter material. 3. The detection device for immunodiagnosis according to claim 1, further comprising a reaction vessel (10), the base (20) and the water-absorbing portion (30) are arranged in the reaction vessel (10) wherein, the reaction vessel (10) has an opening (121), and the opening (121) is located at the top of the reaction vessel (10) and directly above the substrate (20). 4. The detection device for immunodiagnosis according to claim 3, characterized in that, the opening (121) is funnel-shaped. 5. The detection device for immunodiagnosis according to claim 4, characterized in that, the contour of the inner wall surface of the opening (121) matches the contour of the outer wall surface of the detection head of the detector. 6. The detection device for immunodiagnosis according to claim 3, characterized in that an opening (111) is provided at the bottom of the reaction container (10). 7. The detection device for immunodiagnosis according to any one of claims 3 to 6, characterized in that, the reaction container (10) comprises a body (11) and a detachably arranged on the body (11) The cover (12), the opening (121) is arranged on the top of the cover (12). 8. The detection device for immunodiagnosis according to any one of claims 3 to 6, characterized in that a positioning part (40) is provided outside the body (11) of the reaction container (10). 9. The detection device for immunodiagnosis according to claim 8, characterized in that, the positioning part (40) is in the shape of a rectangular ring. 10. The detection device for immunodiagnosis according to any one of claims 1 to 6, characterized in that, the substrate (20) is coated with anti-biotin antibody or streptavidin, which is used to combine with The biotinylated protein added to the substrate (20) specifically binds.