CN108964781A - Multichannel coherent Raman scattering Optical devices and imaging system - Google Patents
Multichannel coherent Raman scattering Optical devices and imaging system Download PDFInfo
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- H—ELECTRICITY
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Abstract
The present invention relates to a kind of multichannel coherent Raman scattering Optical devices and imaging systems, including beam splitter, the first optical module, the second optical module, light intensity modulator and the first bundling device；Seed light is split transmission by beam splitter；Seed light is converted pump light by first optical module；Seed light is converted the mutually different stokes light of wavelength by second optical module；Light intensity modulator is modulated the light intensity of stokes light, each stokes light exports to the first bundling device in the form of light intensity power is staggered and carries out conjunction beam, the coherent Raman scattering signal that the road Shi Ge stokes light and pump light excite the vibration mode of a variety of specific moleculars to generate, realize that multichannel in situ is imaged simultaneously, the working efficiency of improving optical imaging system, replace expensive highly-sensitive detector array, additionally it is possible to avoid multi beam high-peak power light while excite sample that nonlinear optics is caused to be saturated.
Description
Technical Field
The invention relates to the technical field of optics, in particular to a multi-channel coherent Raman scattering optical device and a multi-channel coherent Raman scattering microscopic imaging system.
Background
The coherent Raman scattering spectrum technology is an important technology in the field of optical technology, and comprises a stimulated Raman scattering spectrum technology, a coherent anti-Stokes Raman spectrum technology, a Raman induced Kerr effect and the like, wherein ultrashort pulse laser is mainly used for exciting a nonlinear optical signal of a sample, and then microscopic imaging of the sample is obtained by applying laser scanning and microscopic technology.
When the frequency difference of two or more excitation lights is matched with the characteristic Raman peak of the sample, the radiation of the stimulated Raman scattering signal can be realized, so that the optical signal generated by the sample is greatly enhanced. The currently reported technologies for generating coherent raman scattering signals mainly include three types, the first type is to adopt two phase-locked solid lasers as pump light and stokes light for generating coherent raman scattering; the second one is that an ultrashort pulse laser is provided with an optical parametric oscillator or an optical parametric amplifier to generate pump light and Stokes light; the third is that the ultra-short pulse solid laser or the fiber laser is provided with a nonlinear photonic crystal fiber to generate pump light and Stokes light.
The main problems of the scheme adopting the solid laser are that the system is large in size, complex in structure and high in cost, and the light path is a free light path and poor in stability.
On the other hand, the power of the supercontinuum white light generated by the pumping nonlinear photonic crystal fiber is low, the generated coherent Raman scattering signal is very weak, only a high-sensitivity detector array can be used for detection, and the method is only suitable for coherent anti-Stokes Raman scattering microscopic imaging. Background noise caused by non-resonance signals can be contained in coherent anti-stokes Raman scattering spectra, spectral analysis is not facilitated, a high-sensitivity detector array such as an electronic enhanced charge coupled device is extremely expensive, and the technology is extremely high in cost. For stimulated raman scattering, the spectrum does not contain background noise and the price of the detector is low, however, the measurement technology adopting the detector array cannot realize stimulated raman scattering microscopic imaging at present.
In a word, the current coherent Raman scattering microscopic imaging technology has the advantages of designing a system with compact and stable structure, reducing cost and realizing multi-channel or hyperspectral imaging.
Disclosure of Invention
Based on the method, the multichannel coherent Raman scattering optical device and the multichannel coherent Raman scattering microscopic imaging system are provided for different molecular vibration modes, the simultaneous imaging of multichannel coherent Raman scattering signals is realized, the working efficiency of the system is improved, and an expensive high-sensitivity detector array is replaced.
A multi-channel coherent raman scattering optical device comprising: the device comprises a beam splitter, a first optical component, a second optical component, a light intensity modulator and a first beam combiner; wherein,
the beam splitter is used for splitting the seed light into a pumping branch and at least two Stokes branches for transmission;
the first optical assembly is used for converting the seed light of the pumping branch into pumping light and outputting the pumping light to the first beam combiner;
the second optical assembly is used for converting the seed light of each Stokes branch into Stokes light with different wavelengths;
the light intensity modulator is used for modulating the light intensity of the Stokes light so as to enable each Stokes light to be output to the first beam combiner in a form of staggered light intensity;
the first beam combiner is used for combining the pump light and each Stokes light.
In the multichannel coherent Raman scattering optical device, the beam splitter can receive the seed light and split the seed light into the pumping branch and at least two Stokes branches for transmission, the first optical component converts the seed light transmitted on the pumping branch into the pumping light for generating coherent Raman scattering signals, the second optical component converts the seed light transmitted on each Stokes branch into Stokes light with different frequencies, the light intensity modulator modulates the light intensity of the Stokes light and outputs the Stokes light to the first beam combiner in a form of staggered light intensity for beam combination, so that the Stokes light with staggered light intensity and the pumping light excite coherent Raman scattering signals generated by vibration modes of multiple specific molecules, the simultaneous imaging of multichannel coherent Raman scattering signals is realized, and the working efficiency of an optical imaging system is improved, the method replaces an expensive high-sensitivity detector array, can avoid nonlinear optical saturation caused by simultaneous excitation of a sample by multiple beams of high-peak-power Stokes light, and improves the quality of coherent Raman scattering signals.
In one embodiment, the optical system further comprises a second beam combiner arranged between the second optical component and the light intensity modulator; the second beam combiner is configured to combine the stokes lights with different wavelengths and output the combined stokes lights to the light intensity modulator.
In one embodiment, the optical system further comprises a third beam combiner arranged between the optical intensity modulator and the first beam combiner; the number of the light intensity modulators is multiple, and the light intensity modulators are respectively arranged on the Stokes branches; and the third beam combiner is used for combining the Stokes light output by the light intensity modulators and outputting the Stokes light to the first beam combiner.
In one embodiment, the first optical assembly includes a frequency doubling module disposed on the pump branch; the frequency doubling module is used for performing frequency doubling on the seed light and outputting the seed light as the pump light.
In one embodiment, the first optical assembly further comprises a tuning module disposed between the beam splitter and a frequency doubling module; the tuning module is configured to tune the seed light split by the beam splitter to the pumping branch, and output the seed light to the frequency doubling module.
In one embodiment, the tuning module comprises a photonic crystal fiber and a first narrow-band filter in sequence; the photonic crystal fiber is used for performing frequency expansion on the seed light of the pumping branch and outputting the seed light to the first narrow-band filter; the first narrow-band filter is configured to filter out the seed light with the specified frequency from the seed light after the frequency expansion, and output the seed light to the frequency doubling module.
In one embodiment, the first optical assembly further comprises a first fiber amplifier and a first beam collimator; the first optical fiber amplifier and the first beam collimator are sequentially arranged between the first narrow-band filter and the frequency doubling module; the first optical fiber amplifier is used for amplifying the seed light with the specified frequency filtered by the first narrow-band filter and outputting the seed light to the first beam collimator; the first beam collimator is configured to collimate the amplified seed light and output the collimated seed light to the frequency doubling module.
In one embodiment, the second optical assembly comprises a second narrowband filter, a second optical fiber amplifier, a second beam collimator and an optical path adjusting mechanism in sequence; the second narrow-band filter is used for filtering out stokes light with a specified frequency from the seed light split to the pumping branch by the beam splitter and outputting the stokes light to the second optical fiber amplifier; the second optical fiber amplifier is used for amplifying the Stokes light and outputting the Stokes light to the second beam collimator; the second beam collimator is used for collimating the amplified Stokes light and outputting the collimated Stokes light to the optical path adjusting mechanism; and the optical path adjusting mechanism is used for adjusting the optical paths of the collimated Stokes lights so as to enable the optical paths of the Stokes lights of the Stokes branches to be consistent with the optical paths of the pump lights on the pump branches.
In one embodiment, a multichannel coherent raman scattering microscopy imaging system is provided, comprising a fiber laser, an optical isolator, a multichannel coherent raman scattering optical device as described in any of the above embodiments, a laser galvanometer, a microscope, and an optical signal detection device, arranged in sequence; wherein,
the fiber laser is used for inputting seed light to the optical device through an optical isolator;
the multichannel coherent Raman scattering optical device is used for converting the seed light into pump light for generating a coherent Raman scattering signal and at least two beams of Stokes light, and combining the beams and outputting the combined beams to the laser galvanometer;
the laser galvanometer is used for inputting the combined pump light and Stokes light into the microscope so as to generate a coherent Raman scattering signal at a sample of the microscope;
the optical signal detection device is used for detecting the coherent Raman scattering signal and imaging the coherent Raman scattering signal.
In the multichannel coherent Raman scattering microscopic imaging system, the beam splitter can receive seed light generated by the optical fiber laser and split the seed light into the pumping branch and at least two Stokes branches for transmission, the first optical component converts the seed light transmitted on the pumping branch into the pumping light for generating coherent Raman scattering signals, the second optical component converts the seed light transmitted on each Stokes branch into Stokes lights with different frequencies, the light intensity modulator modulates the light intensity of the Stokes lights and outputs the Stokes lights to the first beam combiner in a form of staggered light intensity for beam combination, so that each Stokes light and the pumping light with staggered light intensity can be focused on a sample of a microscope through the laser galvanometer to enable the sample to generate coherent Raman scattering signals, and then the coherent Raman scattering signals are detected through the optical signal detection device and imaged, the optical fiber laser device can avoid nonlinear optical saturation caused by simultaneous excitation of multiple beams of high-peak power Stokes light on a sample, improve the quality of coherent Raman scattering signals, simultaneously excite the vibration modes of multiple specific molecules of the sample, generate at least two coherent Raman scattering signals, perform in-situ multi-channel imaging, and adopt the optical fiber laser as a seed light source of the coherent Raman scattering signals, so that the optical system is more compact, the structure is simpler, the cost is reduced, and the stability of the optical system is improved.
In one embodiment, the optical signal detection device comprises an optical filter, a photoelectric detector and a signal processor in sequence; the optical filter is used for filtering stray light of the coherent Raman scattering signal and outputting the coherent Raman scattering signal to the photoelectric detector after the stray light is filtered; the photoelectric detector is used for converting the coherent Raman scattering signal into a corresponding electric signal and outputting the electric signal to the signal processor; and the signal processor is used for carrying out imaging processing on the electric signals.
Drawings
FIG. 1 is a schematic diagram of the structure of a multi-channel coherent Raman scattering optical apparatus and an imaging system according to an embodiment;
fig. 2 is a schematic diagram of the modulation principle of the two-channel stokes light in one embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It should be noted that the terms "first \ second \ third" related to the embodiments of the present invention are merely used for distinguishing similar objects, and do not represent a specific ordering for the objects, and it should be understood that "first \ second \ third" may exchange a specific order or sequence order if allowed. It should be understood that the terms first, second, and third, as used herein, are interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in other sequences than those illustrated or otherwise described herein.
In one embodiment, an optical device is provided, which may be used to excite coherent raman scattering signals, and referring to fig. 1, fig. 1 is a schematic structural diagram of a multi-channel coherent raman scattering optical device and an imaging system in one embodiment, the optical device 10 may include: a beam splitter 200, a first optical assembly 300, a second optical assembly 400, an optical intensity modulator 500, and a first beam combiner 600.
The beam splitter 200 is an optical device that splits laser light output by the laser light source 100 according to a certain energy ratio, so that the present embodiment may receive the seed light output by the laser light source 100 and used for exciting a coherent raman scattering signal through the beam splitter 200, and split the received seed light to the pump branch and the at least two stokes branches according to a certain energy ratio through the beam splitter 200 for transmission. The laser light source 100 may use a rare earth element doped glass fiber as a gain medium, the rare earth elements may include neodymium, erbium, ytterbium, holmium, thulium, or the like, and the fiber pulse laser light source may be replaced by a solid pulse laser oscillator, and the pulse repetition frequency range of the fiber pulse laser light source includes 1kHz to 100 MHz.
The first optical component 300 may be disposed on the pump branch, and is mainly configured to convert the seed light split to the pump branch into pump light, and output the converted pump light to the first beam combiner.
The second optical assembly 400 may be disposed on each stokes branch, and is mainly used to convert the seed light of each stokes branch into stokes light with different wavelengths. Taking two stokes branches as an example for illustration, the second optical component 400 may convert the frequency of the seed light of one stokes branch into a first frequency, and convert the frequency of the seed light of the other stokes branch into a second frequency different from the first frequency, and under the condition of multiple stokes branches, may convert each seed light into stokes lights with different frequencies, so as to form multiple stokes lights with different frequencies.
The light intensity modulator 500 is mainly used for modulating the light intensity of the stokes light, the light intensity modulator 500 can independently modulate light with different wavelengths, and the light intensity modulator 500 in this embodiment can respectively modulate the light intensity of the stokes light with different frequencies output by each stokes branch, so that the light intensity is output to the first beam combiner in a form of interleaving of light intensity. Taking a two-channel stokes light as an example for explanation, referring to fig. 2, fig. 2 is a schematic diagram of a modulation principle of the two-channel stokes light in one embodiment, since the frequencies of the two-channel stokes light are different from each other, the optical intensity modulator may modulate the stokes light of one of the channels at a first modulation frequency, while the stokes light of the other channel is modulated at a second modulation frequency different from the first modulation frequency, the light intensities of the Stokes lights output by the two channels are mutually staggered on a time axis and output to the first beam combiner to be coherent with the pump light output by the pump branch, the coherent light with the light intensity changing in a staggered way is output to a subsequent sample, so as to avoid the quality degradation of coherent Raman scattering signals caused by the damage and nonlinear saturation of the sample when the high-power laser is concentrated and input into the sample for subsequent excitation Raman scattering at the same time. Alternatively, the optical intensity modulator 500 may employ an acousto-optic tunable filter or an electro-optic modulator, and the modulation frequency range may include 1kHz to 100 MHz.
The first beam combiner 600 is mainly used for combining the pump light output by the pump branch and each stokes light modulated by light intensity to generate coherent light for exciting a raman scattering signal, and since the light intensities of the stokes light with different frequencies in the coherent light are changed in a staggered manner, the stimulated raman scattering signal of a sample excited by the coherent light output by the first beam combiner 600 can avoid the damage of high-power laser to the sample and the saturation of nonlinear signals, and the quality of the coherent raman scattering signal is improved. The first beam combiner 600 may include a dichroic mirror, a laser beam splitter, or a polarization beam splitter, among others.
In the multichannel coherent Raman scattering optical device, the beam splitter can receive the seed light and split the seed light into the pumping branch and at least two Stokes branches for transmission, the first optical component converts the seed light transmitted on the pumping branch into the pumping light for generating coherent Raman scattering signals, the second optical component converts the seed light transmitted on each Stokes branch into Stokes light with different frequencies, the light intensity modulator modulates the light intensity of the Stokes light and outputs the Stokes light to the first beam combiner in a form of staggered light intensity for beam combination, so that the Stokes light with staggered light intensity and the pumping light excite coherent Raman scattering signals generated by vibration modes of multiple specific molecules, the simultaneous imaging of multichannel coherent Raman scattering signals is realized, and the working efficiency of an optical imaging system is improved, the method replaces an expensive high-sensitivity detector array, can avoid nonlinear optical saturation caused by simultaneous excitation of a sample by multiple beams of high-peak-power Stokes light, and improves the quality of coherent Raman scattering signals.
In one embodiment, a second beam combiner 700 disposed between the second optical assembly 400 and the optical intensity modulator 500 may also be included.
In this embodiment, the second beam combiner 700 is mainly configured to receive stokes light output from each stokes, combine the stokes light with different wavelengths, and output the combined stokes light to the light intensity modulator 500 for light intensity modulation. In this embodiment, the combined stokes lights with different wavelengths are output to the light intensity modulator 500 for light intensity modulation, so that the number of the light intensity modulators 500 can be reduced, the effect of modulating the stokes lights with different wavelengths can be achieved, the light path is more compact, and the layout space of the optical system is saved.
In one embodiment, a third beam combiner disposed between the optical intensity modulator 500 and the first beam combiner 600 may also be included.
In this embodiment, the number of the light intensity modulators 500 may be multiple, and each light intensity modulator 500 is respectively disposed on each stokes branch to independently modulate stokes light on each stokes branch. The third beam combiner is mainly configured to combine stokes light with different wavelengths output by the light intensity modulators 500 on each stokes branch, and output the combined stokes light to the first beam combiner 600.
The technical scheme of the embodiment is mainly that the light intensity modulators 500 are respectively arranged on each stokes branch to realize independent modulation of stokes light of each stokes branch, and then the modulated stokes light of each path is combined and output by using the third beam combiner.
In one embodiment, the first optical assembly may include a frequency doubling module 310 disposed on the pump branch.
In this embodiment, the frequency doubling module 310 is an optical element for performing frequency doubling on the light frequency, and is mainly used to perform frequency doubling on the seed light split by the beam splitter to the pumping branch and output the seed light as pumping light for exciting the raman scattering signal. Optionally, the frequency doubling module 310 may include a focusing optical element 311, a frequency doubling crystal 312 and an optical filter 313, the frequency doubling crystal 312 is disposed at a focal point of the focusing optical element 311, and the focusing optical element 311 is mainly configured to focus the seed light to the frequency doubling crystal 312, so that the frequency doubling crystal 312 performs frequency doubling on the focused seed light and outputs the focused seed light. The focusing optical element 311 may include a converging lens or two concave mirrors, both sides of which are respectively disposed on both sides of the frequency doubling crystal 312, and the frequency doubling crystal 312 may include a barium borate crystal, a lithium triborate crystal, a periodically poled superlattice lithium tantalate crystal, a periodically poled magnesium oxide-doped lithium niobate crystal, or a periodically poled lithium niobate crystal.
In this embodiment, mainly considering that the frequency of the seed light is generally limited by the type of the laser light source 100 and cannot cover the frequency required by the pump light, the technical solution of this embodiment can more conveniently and flexibly convert the seed light wavelength into the required pump light wavelength.
In one embodiment, the first optical assembly 300 may further include a tuning module 320 disposed between the beam splitter 200 and the frequency doubling module 310.
The tuning module 320 is an optical element for adjusting the frequency of the seed light, and is mainly used to tune the seed light split from the beam splitter 200 to the pump branch and output the tuned seed light to the frequency doubling module 310, and optionally, the tuning module 320 may use an optical parametric oscillator or an optical parametric amplifier.
In general, the frequency of the pump light cannot be obtained by only using the frequency doubling module 310 to perform the seed light, because the frequency of the seed light output by the laser light source 100 is relatively single, even if the frequency of the pump light obtained by the frequency doubling module is also single, the single seed light frequency is often difficult to meet the requirement in an application scenario where the frequency of the pump light needs to be adjusted. In this embodiment, the tuning module 320 is adopted to adjust the frequency of the seed light first, and then the frequency doubling module 310 is used to perform frequency doubling processing to obtain the pump light, so that when the frequency of the pump light needs to be adjusted, the tuning module 320 is only needed to be adjusted to quickly obtain the pump light with corresponding frequency through the frequency doubling module 310, thereby further improving the flexibility of the optical device.
In one embodiment, the tuning module 320 may optionally include a photonic crystal fiber 321 and a first narrowband filter 322 in sequence.
The photonic crystal fiber 321 is mainly used for performing spectrum broadening on the seed light split by the beam splitter to the pumping branch to generate, for example, 500 nm to 1700 nm super-continuous white light, and the seed light with a specific frequency is selected by the first narrowband filter 322 and output to the frequency doubling module 310.
In this embodiment, the photonic crystal fiber 321 is further adopted to perform frequency expansion processing on the seed light, so that the seed light generates super-continuous white light, and after the super-continuous white light is generated, the seed light with a specific frequency can be extracted from the frequency spectrum of the super-continuous white light through the first narrow-band filter. When the frequency of the pump light needs to be adjusted, the pump light with the corresponding frequency can be quickly obtained through the frequency doubling module 310 only by adjusting the first narrow-band filter 322 to select the light with the specific frequency, so that the flexibility of the optical device is further improved.
In one embodiment, further, the first assembly 300 may further include a first fiber amplifier 330 and a first beam collimator 340.
The first optical fiber amplifier 330 and the first beam collimator 340 are sequentially disposed between the first narrowband optical filter 322 and the frequency doubling module 310, the first optical fiber amplifier 330 is mainly configured to perform power amplification on the seed light with the designated frequency filtered by the first narrowband optical filter 322, output the seed light to the first beam collimator 340, collimate the seed light with the power amplified by the first beam collimator 340, perform frequency doubling on the collimated seed light by the frequency doubling module 310, and the frequency doubled light can be used as pump light for generating coherent raman scattering signals. Optionally, the first optical fiber amplifier 330 may use a rare earth element doped with neodymium, erbium, ytterbium, holmium, or thulium as a gain medium of the amplifier.
In this embodiment, the seed light may be amplified and collimated, so that the energy of the pump light is improved, and the power-amplified seed light may be collimated and input to the frequency doubling module 310, which ensures that the frequency doubling module 310 can more effectively double the frequency of the seed light to generate high-quality pump light.
In one embodiment, the second optical assembly 400 may include, in order, a second narrowband filter 410, a second fiber amplifier 420, a second beam collimator 430, and an optical path adjusting mechanism 440.
It should be noted that the second optical assembly 400 may be disposed on each stokes branch, and in this embodiment, the second optical assembly 400 is mainly described by one stokes branch. Wherein the second narrowband filter 410 is mainly used for selecting a wavelength of the stokes light of each stokes branch, filtering out the stokes light of a specified frequency from the seed light split from the beam splitter to the pump branch, and outputting the stokes light to the second optical fiber amplifier 420, performing power amplification on the stokes light by using the second optical fiber amplifier 420, and outputting the stokes light to the second beam collimator 430, the second beam collimator 430 may be used for collimating the amplified stokes light, and outputting the collimated stokes light to the optical path length adjusting mechanism 440, and performing precise adjustment on the optical path length of the collimated stokes light, so that the optical path length of the stokes light of each stokes branch is consistent with the optical path length of the pump light on the pump branch, wherein the optical path length adjusting mechanism 440 may include a linear translation stage 441 and a retroreflector 442, and the retroreflector 442 may be performed by using a vertically placed mirror, Hollow retroreflectors, roof prisms, retro-reflective prisms, or right angle prisms.
The technical scheme provided by the embodiment can respectively carry out filtering, amplification, collimation and optical path adjustment on each path of Stokes light, so that the obtained Stokes light signal is stronger, the signal-to-noise ratio is higher, and the multi-channel coherent Raman scattering signal can be simultaneously excited under the combined action of the Stokes light and the pump light.
In an embodiment, a multi-channel coherent raman scattering microscopy imaging system is provided, and referring to fig. 1, fig. 1 is a schematic structural diagram of a multi-channel coherent raman scattering optical device and an imaging system in an embodiment, and the multi-channel coherent raman scattering microscopy imaging system may include: the optical fiber laser 100, the optical isolator 110, the multichannel coherent raman scattering optics 10 as described in any of the above embodiments, the laser galvanometer 120, the microscope 130, and the optical signal detection device 140 are arranged in this order.
In this embodiment, the fiber laser 100 may be configured to generate a multi-channel stokes light for coherent raman scattering imaging and a seed light of a pump light, and the seed light is input into the multi-channel coherent raman scattering optical device 10 through the optical isolator 110, where the optical isolator 110 is configured to eliminate a harmful effect of a backward reflection light on fiber pulse laser light sources such as the fiber laser 100.
The multichannel coherent raman scattering optical device 10 may be configured to convert the seed light output by the optical isolator 110 into a pump light and at least two stokes lights for generating a coherent raman scattering signal, and output a combined beam to the laser galvanometer 120, and enter the microscope 130 body via the laser galvanometer scanning system.
And the laser galvanometer 120 is used for inputting the pump light and the stokes light output by the multi-channel coherent raman scattering optical device 10 beam combination into the microscope 130 so as to generate a coherent raman scattering signal at the sample of the microscope 130. Specifically, the laser galvanometer 120 confocally focuses the pump light and the stokes light in the objective focal plane of the microscope 130 such that a coherent raman scattering nonlinear optical signal is excited at the sample. The microscope 130 may be a light emission microscope or a light transmission microscope, or a commercial microscope or a home-made microscope, the range of the magnification of the objective lens of the microscope 130 includes 10 times to 100 times, and the range of the numerical aperture includes 0.1 to 1.49.
The optical signal detection device 140 is a device for collecting and processing an optical signal, and is mainly used for detecting a coherent raman scattering signal and imaging the coherent raman scattering signal, and the optical signal detection device 140 may collect a coherent raman scattering nonlinear optical signal excited at a sample through a condenser lens.
The embodiment adopts the ultrashort pulse fiber laser as an excitation source of coherent Raman scattering, so that the system structure is more compact, the stability is higher, the cost of a light source is greatly reduced, the respective modulation of multiple stokes light beams is adopted, the synchronous coherent Raman scattering microscopic imaging of two channels or more channels is realized aiming at different vibration modes of multiple specific molecules, an expensive high-sensitivity detector array is replaced, the saturation of nonlinear optical signals is avoided, and the working efficiency of the system is greatly improved.
Optionally, the optical signal detection device 140 may include an optical filter 141, a photodetector 142, and a signal processor 143 in sequence. The optical filter 141 may be configured to filter stray light of the coherent raman scattering signal, and output the coherent raman scattering signal after the stray light is filtered to the photodetector 142; the photodetector 143 may be configured to convert the coherent raman scattering signal into a corresponding electrical signal, and output the corresponding electrical signal to the signal processor, where the signal processor is configured to perform imaging processing on the electrical signal, and for stimulated raman scattering microscopic imaging, the signal processor may perform phase-locked amplification on two modulation frequency signals, so as to extract nonlinear optical signals with different frequencies, thereby implementing multichannel synchronous coherent raman imaging. The same principle can be applied to coherent anti-stokes Raman scattering imaging and Raman induced Kerr effect.
For the stimulated Raman scattering signals, the photoelectric detector can adopt an optical bias detector, a photodiode detector or a detector array, an optical filter is arranged in front of the photoelectric detector to filter out the modulated Stokes light, and then a phase-locked amplifier, a data acquisition card or an averager is adopted to extract the stimulated Raman scattering signals. For the raman induced kerr effect signal, the same detection mode can be adopted, and an analyzer is additionally arranged in front of the detector.
For coherent anti-stokes raman scattering signals, the optical detector can adopt a high-sensitivity detector, such as a photomultiplier, a single photon counting multiplier, a single photon counting avalanche diode detector, a charge coupled device, or an electron enhanced charge coupled device, etc., a short-pass filter is placed in front of the detector to filter out stokes light and pump light, and then a phase-locked amplifier, a data acquisition card, or a photon counter, etc., are adopted to measure the coherent anti-stokes raman signals.
The multi-channel coherent Raman scattering microscopic imaging system can be applied to label-free biological imaging and molecular detection, such as tissue slice sample imaging, cell imaging, human body dynamic imaging and the like; the beam splitter can receive seed light generated by the optical fiber laser and split the seed light into the pumping branch and at least two stokes branches for transmission, the first optical component converts the seed light transmitted on the pumping branch into pumping light for generating coherent Raman scattering signals, the second optical component converts the seed light transmitted on each stokes branch into stokes lights with different frequencies, the light intensity modulator modulates the light intensity of the stokes lights and outputs the stokes lights to the first beam combiner in a form of staggered light intensity for beam combination, so that the stokes lights with staggered light intensity and the pumping light can be focused on a sample of the microscope through the laser vibrating mirror to enable the sample to generate coherent Raman scattering signals, and the coherent Raman scattering signals generated by vibration modes of multiple specific molecules of the sample can be detected simultaneously, the in-situ multi-channel simultaneous imaging is realized, an expensive high-sensitivity detector array is replaced, the working efficiency of the system is greatly improved, the nonlinear optical saturation caused when multiple beams of high-peak power Stokes light simultaneously excite a sample can be avoided, the quality of coherent Raman scattering signals is improved, and the fiber laser is adopted as a seed light source of the coherent Raman scattering signals, so that the optical system is more compact, the structure is simpler, the cost is reduced, and the stability of the optical system is improved.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A multi-channel coherent raman scattering optical device, comprising: the device comprises a beam splitter, a first optical component, a second optical component, a light intensity modulator and a first beam combiner; wherein,
the beam splitter is used for splitting the seed light into a pumping branch and at least two Stokes branches for transmission;
the first optical assembly is used for converting the seed light of the pumping branch into pumping light and outputting the pumping light to the first beam combiner;
the second optical assembly is used for converting the seed light of each Stokes branch into Stokes light with different wavelengths;
the light intensity modulator is used for modulating the light intensity of the Stokes light so as to enable each Stokes light to be output to the first beam combiner in a form of staggered light intensity;
the first beam combiner is used for combining the pump light and each Stokes light.
2. The multi-channel coherent raman scattering optical device of claim 1, further comprising a second beam combiner disposed between the second optical assembly and the optical intensity modulator; wherein,
and the second beam combiner is used for combining the Stokes lights with different wavelengths and outputting the Stokes lights to the light intensity modulator.
3. The multi-channel coherent raman scattering optical device of claim 1, further comprising a third beam combiner disposed between the optical intensity modulator and the first beam combiner; the number of the light intensity modulators is multiple, and the light intensity modulators are respectively arranged on the Stokes branches;
and the third beam combiner is used for combining the Stokes light output by the light intensity modulators and outputting the Stokes light to the first beam combiner.
4. The multi-channel coherent raman scattering optical device of claim 1, wherein the first optical assembly comprises a frequency doubling module disposed on the pump branch; wherein,
and the frequency doubling module is used for performing frequency doubling on the seed light and outputting the seed light as the pump light.
5. The multichannel coherent raman scattering optical device of claim 4, wherein the first optical assembly further comprises a tuning module disposed between the beam splitter and a frequency doubling module; wherein,
and the tuning module is used for tuning the seed light split by the beam splitter to the pumping branch and outputting the seed light to the frequency doubling module.
6. The multi-channel coherent raman scattering optical device of claim 5, wherein the tuning module comprises, in order, a photonic crystal fiber and a first narrow band filter; wherein,
the photonic crystal fiber is used for performing frequency expansion on the seed light of the pumping branch and outputting the seed light to the first narrow-band optical filter;
the first narrow-band filter is configured to filter out the seed light with the specified frequency from the seed light after the frequency expansion, and output the seed light to the frequency doubling module.
7. The multi-channel coherent raman scattering optical device of claim 6, wherein the first optical assembly further comprises a first fiber amplifier and a first beam collimator; wherein,
the first optical fiber amplifier and the first beam collimator are sequentially arranged between the first narrow-band filter and the frequency doubling module;
the first optical fiber amplifier is used for amplifying the seed light with the specified frequency filtered by the first narrow-band filter and outputting the seed light to the first beam collimator;
the first beam collimator is configured to collimate the amplified seed light and output the collimated seed light to the frequency doubling module.
8. The multi-channel coherent raman scattering optical device of claim 1, wherein the second optical assembly comprises, in order, a second narrowband filter, a second fiber amplifier, a second beam collimator, and an optical path adjusting mechanism; wherein,
the second narrow-band filter is used for filtering out Stokes light with a specified frequency from the seed light split by the beam splitter to the pumping branch and outputting the Stokes light to the second optical fiber amplifier;
the second optical fiber amplifier is used for amplifying the Stokes light and outputting the Stokes light to the second beam collimator;
the second beam collimator is used for collimating the amplified Stokes light and outputting the collimated Stokes light to the optical path adjusting mechanism;
and the optical path adjusting mechanism is used for adjusting the optical paths of the collimated Stokes lights so as to enable the optical paths of the Stokes lights of the Stokes branches to be consistent with the optical paths of the pump lights on the pump branches.
9. A multichannel coherent raman scattering microscopy system comprising a fiber laser, an optical isolator, a multichannel coherent raman scattering optical device according to any one of claims 1 to 8, a laser galvanometer, a microscope and an optical signal detection device arranged in sequence; wherein,
the fiber laser is used for inputting seed light to the optical device through an optical isolator;
the multichannel coherent Raman scattering optical device is used for converting the seed light into pump light for generating a coherent Raman scattering signal and at least two beams of Stokes light, and combining the beams and outputting the combined beams to the laser galvanometer;
the laser galvanometer is used for inputting the combined pump light and Stokes light into the microscope so as to generate a coherent Raman scattering signal at a sample of the microscope;
the optical signal detection device is used for detecting the coherent Raman scattering signal and imaging the coherent Raman scattering signal.
10. The multi-channel coherent raman scattering microscopy imaging system according to claim 9, wherein the optical signal detection device comprises, in order, an optical filter, a photodetector and a signal processor; wherein,
the optical filter is used for filtering stray light of the coherent Raman scattering signal and outputting the coherent Raman scattering signal to the photoelectric detector after the stray light is filtered;
the photoelectric detector is used for converting the coherent Raman scattering signal into a corresponding electric signal and outputting the electric signal to the signal processor;
and the signal processor is used for carrying out imaging processing on the electric signals.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109765213A (en) * | 2019-03-27 | 2019-05-17 | 威朋（苏州）医疗器械有限公司 | Coherent anti-stokes raman scattering microscope imaging device |
| CN111239101A (en) * | 2020-02-20 | 2020-06-05 | 云南电网有限责任公司电力科学研究院 | Detection system of oil consumption device |
| CN112240880A (en) * | 2020-09-18 | 2021-01-19 | 华中科技大学 | Super-resolution stimulated Raman microscopic imaging method and device for realizing near resonance enhancement |
| CN116879208A (en) * | 2023-09-08 | 2023-10-13 | 星元极光(苏州)光电科技有限公司 | Transient absorption spectrum measuring method and device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2157415A1 (en) * | 2008-08-22 | 2010-02-24 | President And Fellows Of Harvard College | Microscopy imaging system and method employing stimulated Raman spectroscopy as a contrast mechanism |
| CN102623874A (en) * | 2012-04-05 | 2012-08-01 | 清华大学 | Laser source device and generation method of coherent Raman scattering microscope system |
| CN103487146A (en) * | 2013-09-16 | 2014-01-01 | 华南师范大学 | Ultra wide band stimulated raman spectroscopy microscopic imaging system simple and convenient to use |
| CN106226284A (en) * | 2016-08-23 | 2016-12-14 | 深圳大学 | Coherent anti-stokes raman scattering spectrographic detection and micro imaging system and method |
| CN206479454U (en) * | 2016-12-27 | 2017-09-08 | 深圳大学 | Coherent anti-stokes raman scattering micro imaging system |
| CN208924248U (en) * | 2018-08-13 | 2019-05-31 | 广州光智科技有限公司 | Multichannel coherent Raman scattering optical system and imaging system |
-
2018
- 2018-08-13 CN CN201810915540.0A patent/CN108964781A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2157415A1 (en) * | 2008-08-22 | 2010-02-24 | President And Fellows Of Harvard College | Microscopy imaging system and method employing stimulated Raman spectroscopy as a contrast mechanism |
| CN102623874A (en) * | 2012-04-05 | 2012-08-01 | 清华大学 | Laser source device and generation method of coherent Raman scattering microscope system |
| CN103487146A (en) * | 2013-09-16 | 2014-01-01 | 华南师范大学 | Ultra wide band stimulated raman spectroscopy microscopic imaging system simple and convenient to use |
| CN106226284A (en) * | 2016-08-23 | 2016-12-14 | 深圳大学 | Coherent anti-stokes raman scattering spectrographic detection and micro imaging system and method |
| CN206479454U (en) * | 2016-12-27 | 2017-09-08 | 深圳大学 | Coherent anti-stokes raman scattering micro imaging system |
| CN208924248U (en) * | 2018-08-13 | 2019-05-31 | 广州光智科技有限公司 | Multichannel coherent Raman scattering optical system and imaging system |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109765213A (en) * | 2019-03-27 | 2019-05-17 | 威朋（苏州）医疗器械有限公司 | Coherent anti-stokes raman scattering microscope imaging device |
| WO2020192153A1 (en) * | 2019-03-27 | 2020-10-01 | 威朋（苏州）医疗器械有限公司 | Coherent anti-stokes raman scattering microscope imaging apparatus |
| JP2022528951A (en) * | 2019-03-27 | 2022-06-16 | 威朋（蘇州）医療器械有限公司 | Coherent anti-Stoke Raman scattering microscope imaging device |
| JP7203464B2 (en) | 2019-03-27 | 2023-01-13 | 威朋（蘇州）医療器械有限公司 | Coherent anti-Stokes Raman scattering microscope imaging system |
| US11879780B2 (en) | 2019-03-27 | 2024-01-23 | Weipeng (Suzhou) Medical Devices Co., Ltd. | Coherent anti-Stokes Raman scattering microscope imaging apparatus |
| CN109765213B (en) * | 2019-03-27 | 2024-03-29 | 苏州威邦震电光电技术有限公司 | Coherent anti-stokes raman scattering microscope imaging device |
| CN111239101A (en) * | 2020-02-20 | 2020-06-05 | 云南电网有限责任公司电力科学研究院 | Detection system of oil consumption device |
| CN112240880A (en) * | 2020-09-18 | 2021-01-19 | 华中科技大学 | Super-resolution stimulated Raman microscopic imaging method and device for realizing near resonance enhancement |
| CN116879208A (en) * | 2023-09-08 | 2023-10-13 | 星元极光(苏州)光电科技有限公司 | Transient absorption spectrum measuring method and device |
| CN116879208B (en) * | 2023-09-08 | 2023-11-28 | 星元极光(苏州)光电科技有限公司 | Transient absorption spectrum measuring method and device |
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