CN108934272A - A method of promote Jiangnan keteleeria fortunei seed to sprout - Google Patents

Abstract

The invention discloses a method for promoting the germination of the seeds of Chinese fir, which comprises the following steps: (1) Seed selection: the selected shape is oval, the front is brown and shiny, the back is light yellow, and the middle part of the seed wing is wider or the middle and lower part Wider seeds with a thousand-grain weight of 130‑155 g, and then flotation to get plump seeds; (2) Seed disinfection: Soak the plump seeds obtained from the selection with potassium permanganate solution, and then rinse with distilled water; (3 ) Pregermination treatment: Soak the sterilized seeds with a concentration of 50‑300 mg/L gibberellin solution for 12‑48 h at a temperature of 25‑40 °C; (4) Seed germination: place the seeds after the germination treatment Placed in a Petri dish covered with 2 layers of filter paper, and then placed in an incubator for cultivation. This method can significantly improve the seed germination rate and quality of seedlings of Yale fir, shorten the germination time of the seeds, and provides a scientific and reliable method for the large-scale cultivation and standardized production of Yale fir.

Description

A kind of method for promoting the seed germination of Chinese fir

technical field

The invention belongs to the technical field of plant cultivation, and in particular relates to a method for promoting seed germination of Chinese fir.

Background technique

Jiangnan oil fir (Keteleeria fortunei var. cyclolepis) belongs to Pinaceae (Pinaceae) Keteleeria (Keteleeria). , Guizhou and other provinces. Jiangnan oil cedar has a tall and straight trunk, dense green branches and leaves, dense material, beautiful material color, durable, and the root contains gum. It is often used as paper-making glue in the folk. Therefore, Jiangnan oil fir is a very valuable timber tree species and landscaping ornamental trees. At the same time, due to its excellent characteristics such as strong stress resistance, fast growth and strong adaptability, Yale fir is an excellent tree species for mountain afforestation. In recent years, due to comprehensive factors such as man-made deforestation, its own biological characteristics, and natural environment, the distribution range of Jiangnan yew fir has shrunk, and the wild number has decreased sharply. It has been listed as a local protected tree species by provinces and regions.

In order to alleviate the contradiction between resource shortage and development and utilization, artificial cultivation is an effective way. In the previous work on planting resource investigation and breeding of Yale fir, it was found that the seed setting rate of Yale fir population was low, and the rate of seed abortion was as high as 80%. These conditions seriously restrict the standardized planting and production of Yale fir. On the other hand, due to the large differences in the plant attributes and seed physiological habits of different plants, the methods suitable for their seed germination are also different, so there is no method suitable for promoting the seed germination of Chinese fir, especially for Chinese fir. . Therefore, in order to promote the large-scale cultivation and standardized production of Yale fir, it is imminent to study the methods of shortening the seed germination time and improving the seed germination rate and seedling quality of Yale fir.

Contents of the invention

The present invention aims at the shortcomings of low germination rate and long germination time of the seeds of Yale fir in the prior art, and aims at the characteristics of hard seed coat, poor water permeability and rich in oil of the seeds of Yale fir, and provides a method for promoting the germination of Chinese fir seeds. The method can promote the seed germination of Chinese fir, shorten the seed germination time, improve the seed germination rate and seedling quality.

The present invention is realized by adopting the following technical solutions:

A method for promoting seed germination of Chinese fir chinensis, comprising the following steps:

(1) Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 130-155 g, mix them with water, and thoroughly After stirring, remove the floating empty and shriveled seeds, then add sodium hydroxide to the water to soak and scrub to get plump seeds;

(2) Seed disinfection: soak the plump seeds obtained from the selection with 0.2% potassium permanganate solution for 10-15 min, and then rinse with distilled water for 3-5 times;

(3) Pregermination treatment: Soak the sterilized seeds in a solution of 50-300 mg/L gibberellin at a temperature of 25-40°C for 12-48 h;

(4) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and Under the condition of 12 h darkness and light intensity of 1000 LX, the culture was carried out continuously with 24 h as a cycle.

For further optimization, in the above step (1) seed selection, add 0.2 g sodium hydroxide and 1.5 U alkaline lipase per 100 ml of water, soak the seeds for 12 hours, then scrub the seeds, and remove the sunken seeds to obtain plump seeds .

Further optimization, the above step (3) germination treatment, soak the sterilized seeds with a concentration of 200 mg/L gibberellin solution at a temperature of 30 °C for 48 h.

Compared with the prior art, this technical solution has the following beneficial effects:

1. In the seed selection step of the method of the present invention, the selected shape is oval, the front side is brown and shiny, the back side is light yellow, the middle part of the seed wing is wider or the middle and lower part is wider, and the thousand-grain weight of the seed is required to reach 130-155g . Among them, the thousand-grain weight is an important indicator reflecting the size and fullness of the seeds, and it is also an important basis for measuring the quality of the seeds. This method screens seeds according to the shape, color, seed wing shape and thousand-grain weight of the seeds, which can effectively ensure that the seeds with good plumpness and excellent quality are screened, and the germination rate of the seeds is improved; adding sodium hydroxide and alkaline lipase to soak and scrub the seeds , can effectively remove a large amount of oil in the seed coat, and soften the seed coat at the same time, and the addition of alkaline lipase can also reduce the amount of sodium hydroxide, and improve the safety of the method.

2. In the germination-promoting treatment step of the method of the present invention, aiming at the characteristics of hard seed coat, poor water permeability, rich in oil, slow germination and low germination rate, the seeds after soaking and sterilizing with gibberellin solution of suitable concentration are used , and scientifically control the soaking temperature and time, which not only promotes the germination of the seeds of Chinese fir, but also shortens the germination time of the seeds.

3, in the seed germination step of the inventive method, cultivate seed under the humidity condition of 90%, guarantee that the Chinese cedar tree seed fully absorbs water when germination, promotes its seed coat expansion and softening, increases oxygen penetration, strengthens each seed of the seed. Physiological activities, improve the absorption and utilization of nutrients stored in the seeds, increase the germination rate of seeds, and shorten the germination time of seeds.

4. According to the experimental data, it is proved that the method of the present invention can not only improve the germination rate of the seeds and shorten the germination time of the seeds, but also can improve the germination potential, germ length and radicle length of the Cedar chinensis seeds compared with the prior art. Thus it can be seen that the present invention can comprehensively improve the vitality of seeds and improve the quality of seedlings.

5. The method of the present invention is suitable for promoting the germination of the seeds of Yale fir, has simple operation, low cost, and is easy to popularize, and meets the requirements of large-scale cultivation and standardized production of Yale fir.

Detailed ways

The present invention is further illustrated by the following examples, but not as a limitation of the present invention. For the specific experimental conditions and methods not indicated in the following examples, the technical means used are generally conventional means well known to those skilled in the art.

Example 1:

A method for promoting seed germination of Chinese fir chinensis, comprising the following steps:

(1) Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 130 g, mix them with water, and stir them thoroughly Remove floating empty and shriveled seeds, then add 0.2 g sodium hydroxide and 1.5 U alkaline lipase per 100 ml of clean water, soak for 12 hours, then scrub the seeds, and remove the sunken seeds to obtain plump seeds;

(2) Seed disinfection: soak the plump seeds obtained by selection with 0.2% potassium permanganate solution for 12 minutes, and then rinse them with distilled water 4 times;

(3) Acceleration treatment: Soak the sterilized seeds in a 200 mg/L gibberellin solution at a temperature of 30°C for 48 h;

(4) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and Under the condition of 12 h darkness and light intensity of 1000 LX, the culture was carried out continuously with 24 h as a cycle.

Example 2:

A method for promoting seed germination of Chinese fir chinensis, comprising the following steps:

(1) Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 135 g, mix them with water, and stir them thoroughly Remove floating empty and shriveled seeds, then add 0.2 g sodium hydroxide and 1.5 U alkaline lipase per 100 ml of clean water, soak for 12 hours, then scrub the seeds, and remove the sunken seeds to obtain plump seeds;

(2) Seed disinfection: soak the plump seeds obtained by the selection with 0.2% potassium permanganate solution for 10 minutes, and then rinse with distilled water for 3 times;

(3) Acceleration treatment: Soak the sterilized seeds in a 200 mg/L gibberellin solution for 36 hours at a temperature of 25°C;

(4) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and Under the condition of 12 h darkness and light intensity of 1000 LX, the culture was carried out continuously with 24 h as a cycle.

Example 3:

A method for promoting seed germination of Chinese fir chinensis, comprising the following steps:

(1) Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 155 g, mix them with water, and stir them thoroughly Remove floating empty and shriveled seeds, then add 0.2 g sodium hydroxide and 1.5 U alkaline lipase per 100 ml of clean water, soak for 12 hours, then scrub the seeds, and remove the sunken seeds to obtain plump seeds;

(2) Seed disinfection: soak the plump seeds obtained by selection with 0.2% potassium permanganate solution for 15 minutes, and then rinse them with distilled water for 5 times;

(3) Acceleration treatment: Soak the sterilized seeds in a 100 mg/L gibberellin solution at a temperature of 35°C for 48 h;

(4) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and Under the condition of 12 h darkness and light intensity of 1000 LX, the culture was carried out continuously with 24 h as a cycle.

Example 4:

A method for promoting seed germination of Chinese fir chinensis, comprising the following steps:

(1) Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 145 g, mix them with water, and stir them thoroughly Remove floating empty and shriveled seeds, then add 0.2 g sodium hydroxide and 1.5 U alkaline lipase per 100 ml of clean water, soak for 12 hours, then scrub the seeds, and remove the sunken seeds to obtain plump seeds;

(2) Seed disinfection: Soak the plump seeds obtained by the selection with 0.2% potassium permanganate solution for 13 minutes, then rinse with distilled water 4 times;

(3) Pregermination treatment: Soak the sterilized seeds with a concentration of 50 mg/L gibberellin solution at a temperature of 40°C for 48 hours;

(4) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and Under the condition of 12 h darkness and light intensity of 1000 LX, the culture was carried out continuously with 24 h as a cycle.

Example 5:

A method for promoting seed germination of Chinese fir chinensis, comprising the following steps:

(1) Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 140 g, mix them with water, and stir them thoroughly Remove floating empty and shriveled seeds, then add 0.2 g sodium hydroxide and 1.5 U alkaline lipase per 100 ml of clean water, soak for 12 hours, then scrub the seeds, and remove the sunken seeds to obtain plump seeds;

(2) Seed disinfection: Soak the plump seeds obtained by selection with 0.2% potassium permanganate solution for 10 minutes, and then rinse them with distilled water for 5 times;

(3) Pregermination treatment: Soak the sterilized seeds in a 200 mg/L gibberellin solution at a temperature of 35°C for 12 h;

(4) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and Under the condition of 12 h darkness and light intensity of 1000 LX, the culture was carried out continuously with 24 h as a cycle.

Embodiment 6:

A method for promoting seed germination of Chinese fir chinensis, comprising the following steps:

(1) Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 150g, mix them with water, and remove them after fully stirring For floating empty and shriveled seeds, add 0.2 g sodium hydroxide and 1.5 U alkaline lipase per 100 ml of clean water, soak for 12 hours, then scrub the seeds, and remove the sunken seeds to obtain plump seeds;

(2) Seed disinfection: soak the plump seeds obtained by the selection with 0.2% potassium permanganate solution for 15 minutes, and then rinse with distilled water for 3 times;

(3) Acceleration treatment: Soak the sterilized seeds in a 300 mg/L gibberellin solution at a temperature of 35°C for 24 h;

(4) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and Under the condition of 12 h darkness and light intensity of 1000 LX, the culture was carried out continuously with 24 h as a cycle.

Test example:

Orthogonal experiment was designed, and the concentration of gibberellin solution, soaking time and soaking temperature were used as factors to determine the germination rate, germination potential, germination index, germination time lag, germ length, and embryo root length. The specific steps of the test are as follows:

(1) Seed source: The tested seeds come from the well-growing Jiangnan yew forest in the Liupai sub-field of Daguishan Forest Farm in Hezhou City. The cones were collected in November 2015, and then the cones were stacked indoors for several days. After the seeds in the cones were fully mature, they were spread out in the sun, and the seeds came out naturally. After drying, they were stored in a refrigerator at 4°C;

(2) Seed selection: select the plump seeds for the test according to the seed selection method described in the above-mentioned embodiment 1;

(3) Seed disinfection: carry out seed disinfection according to the seed disinfection method described in the above-mentioned embodiment 1;

(4) Accelerating treatment: The seeds of Chinese fir chinensis were accelerated before germination by using gibberellin solution×soaking temperature×soaking time. Taking the concentration of gibberellin solution, soaking time, and soaking temperature as factors, the experiments were arranged at 4 levels for each factor (Table 1), and a total of 16 treatments were used (Table 2). Soaking in clean water for 12 hours at room temperature was used as the control (CK);

Table 1 Factor level list of germination test

Table 2 Experimental combination of germination treatment

(5) Seed germination: The seeds after germination treatment were placed in a petri dish covered with 2 layers of filter paper, 30 seeds were placed in each petri dish, each treatment was repeated 3 times, and then placed at a temperature of 25°C. The humidity was 90%, the light intensity was 1000 LX, and the alternating light time was 12 h light/12 h dark incubator for cultivation;

(6) Seed germination observation: observe once every 24 hours, when the radicle reaches 1/2 of the seed length, it is considered germination, and the observation lasts for 30 days. Count germination indicators such as seed germination rate, germination potential, germination index, germination time lag, and length of germ and radicle. The calculation formula and description of each indicator are as follows:

Germination rate (%)=(total number of germinated seeds/number of tested seeds)×100%;

Germination potential (%)=(Number of normal germinated seeds within 10 days/Number of tested seeds)×100%;

Germination index=∑(Gt/Dt), wherein Gt is the number of germinated seeds in t days, and Dt is the number of days to germinate;

Germination lag (d): the number of days required for the first seed to germinate;

Germ and radicle length (mm): After the seeds germinate, select 10 germinated seeds from each petri dish to measure the length of the germ and radicle respectively (if less than 10, measure all);

(7) Measurement results: There are extremely significant differences in the germination rate, germination potential, germination index, germination time lag, germ length, and radicle length of Cedar chinensis seeds under different germination treatments (Table 3);

Table 3 ANOVA and multiple comparisons of seed germination among different germination treatments

Note: "**" means extremely significant difference at P = 0.05 level; different letters in the same column mean significant difference at P = 0.05 level, same letters mean no significant difference.

(8) Conclusion: The seed treatment method used in the present invention is compared with the control method, and the germination of the seeds is greatly shortened, and the germination potential, germination index, germ length and radicle length are all better than the control group, especially under the condition of treatment 10 Jiangnan oil The germination rate, germination potential, germination index, germ length, and radicle length of Chinese fir seeds were the largest, respectively 83.33 %, 56.67 %, 28.32, 20.05 mm, and 41.35 mm; compared with the control (CK) treatment, the germination rate, germination potential, The germination index, germ length, and radicle length increased by 20%, 26.67%, 14.56%, 8.36 mm, and 9.77 mm, respectively; the germination time lag of seeds under treatment 10 was shortened by 3.66 days compared with the control treatment. Thus it can be seen that the method of the present invention can significantly improve the seed germination rate and seedling quality of Yale fir, shorten the germination time of the seeds, and provide a scientific and reliable method for promoting the large-scale cultivation and standardized production of Yale fir.

In addition, it should be understood that although this specification is described according to implementation modes, not each implementation mode only contains an independent technical solution, and this description in the specification is only for clarity, and those skilled in the art should take the specification as a whole , the technical solutions in the various embodiments can also be properly combined to form other implementations that can be understood by those skilled in the art.

Claims (3)

1. a method for promoting the seed germination of Chinese fir, is characterized in that: it may further comprise the steps: Seed selection: Select seeds that are oval in shape, brown and shiny on the front, light yellow on the back, wide in the middle or lower part of the seed wings, and have a thousand-grain weight of 130-155 g. Mix them with clean water, stir well and remove Floating empty and shriveled seeds, then add sodium hydroxide and alkaline lipase to the water to soak and scrub to get plump seeds; Seed disinfection: soak the plump seeds obtained by selection with 0.2% potassium permanganate solution for 10-15min, then rinse with distilled water for 3-5 times; Germination treatment: Soak the sterilized seeds with a concentration of 50-300 mg/L gibberellin solution at a temperature of 25-40°C for 12-48 hours; Seed germination: The seeds after germination treatment were placed in a Petri dish covered with 2 layers of filter paper, and then placed in an incubator at a temperature of 25 °C, a humidity of 90%, and an alternating light time of 12 h light and 12 h dark , under the condition of light intensity of 1000 LX, continuous culture was carried out with 24 h as a cycle. 2. A method for promoting the germination of Cedars chinensis seeds according to claim 1, characterized in that: in the step (3), the sterilized seeds are sterilized at a temperature of 200 mg/L gibberellin solution Soak for 48 hours at 30°C. 3. A method for promoting the germination of Chinese fir seeds according to claim 1, characterized in that: in the step (1), 0.2 g of sodium hydroxide and 1.5 U of alkaline lipase are added to every 100 ml of clear water After soaking for 12 hours, scrub the seeds, remove the seeds that sink to the bottom, and get full seeds.