CN108902325A - Compound packet of a kind of baby formula milk powder with prebiotic function and preparation method thereof - Google Patents

Abstract

The present invention relates to the baby formula milk powders and preparation method thereof that baby formula milk powder field, in particular to a kind of pair of enteron aisle have prebiotic function.In the milk powder, by testing in vivo and in vitro, the mixed proportion of galactooligosaccharide and the compound packet of polydextrose is proposed and demonstrated 4:1 or 9:In 1 range, when the total addition level of preferred oligosaccharide is 5.0 parts by weight/100 parts by weight milk powder, there are obvious probiotic effects to enteron aisle.And taste smell, brew, structural state and the color of babies ' formula milk powder are not adversely affected, but clearly enhance instant capacity.Formula milk of the invention can adjust baby intestinal flora, have the function of relax bowel and defecation and improve immunity of organisms.

Description

A compound package of infant formula milk powder with probiotic function and preparation method thereof

technical field

The invention relates to a compound package of infant formula milk powder which is convenient to use and has a probiotic function and a preparation method of formula milk powder, in particular to an infant formula milk powder for regulating intestinal function.

Background technique

Infancy is the basic period for the establishment of intestinal flora, and it is also a critical period. The intestinal flora can be regarded as an organ of the human body, which can inhibit the growth of pathogenic bacteria, prevent diseases, and improve the body's immunity. Therefore, it is very important to regulate the balance of intestinal flora in infancy. For infants who are breastfed, since the dietary fiber in breast milk can stimulate the growth of beneficial bacteria and inhibit the proliferation of harmful bacteria, it can balance the intestinal flora during infancy. However, the rate of human breastfeeding is declining year by year, causing newborns to use baby food to make up for the lack of dietary fiber in breast milk as much as possible. Both Bifidobacterium and Lactobacillus acidophilus are very important bacteria in the intestinal flora of infants and young children. The number of the two in the intestinal tract is an important indicator for evaluating the health of the body, because they have good health functions, such as adhesion to the human body. Epithelial cells promote lactose tolerance and digestion, etc., but how to effectively proliferate both at the same time is a problem for researchers.

At present, some prebiotics are usually added to achieve the proliferation of beneficial bacteria, usually including galacto-oligosaccharides, polydextrose, isomalto-oligosaccharides, xylo-oligosaccharides, fructo-oligosaccharides, etc. are commonly used, but which prebiotics or what kind of prebiotics How to compound can effectively proliferate and co-exist with Bifidobacterium and Lactobacillus acidophilus at the same time, which is still blank at present.

Galacto-oligosaccharides are mainly used in formula milk powder, fermented milk and milk drinks in food. In the field of formula milk powder, galacto-oligosaccharides are mainly used in infant formula milk powder or milk powder for middle-aged and elderly people. The intestinal flora of artificially fed infants is very different from that of breastfed infants, and is often accompanied by constipation, which can be well prevented by adding dietary fiber such as galacto-oligosaccharides to infant formula. Galacto-oligosaccharides can not only improve digestion, but also produce vitamin B to make foods added with galacto-oligosaccharides more nutritious, so that baby food added with galacto-oligosaccharides can not only simulate the composition of breast milk, but also promote human health in many ways. healthy. However, adding galacto-oligosaccharides alone, due to its strong moisture retention, usually increases the cohesiveness of the milk powder during the preparation process and affects the instant solubility of the milk powder.

Polydextrose is also one of the common types of dietary fiber. Polydextrose is a product of random polymerization. It has many types of glycosidic bonds and a complex molecular structure, which can increase the growth and reproduction of beneficial bacteria in the body. It has been widely used in the food industry at home and abroad. It is widely used in dairy products such as infant formula milk powder or special medical food. Polydextrose has the common functions of other dietary fibers, such as: promoting the growth of probiotics such as bifidobacteria and lactic acid bacteria in the intestine, maintaining the balance of intestinal flora; improving intestinal function, reducing the generation of intestinal spoilage, relieving constipation, Prevent diarrhea; reduce blood sugar response, etc. Polydextrose is a slowly fermented dietary fiber that is easily soluble in water.

Although the above-mentioned prebiotics such as galacto-oligosaccharides, polydextrose, isomalto-oligosaccharides, xylo-oligosaccharides, and fructo-oligosaccharides are widely used in infant formula milk powder, they are all added alone or mixed to play their own roles or functions. Superposition does not bring into play the mutual synergy and complementary effect between the prebiotic components; and in the process of preparing milk powder, it is inconvenient to weigh and add together with other raw materials.

Therefore, it is of more practical significance to develop a prebiotic compound package of dietary fiber with specific components and proportions for the preparation of infant formula milk powder with significant probiotic functions.

Contents of the invention

One of the objects of the present invention is to provide a compound package of prebiotics for infant formula milk powder with probiotic function on the intestinal tract and an infant formula milk powder prepared by using the compound package.

The second object of the present invention is to verify the different dietary fibers in infant formula milk powder by studying the influence of dietary fiber in infant formula milk powder on the number of bifidobacterium and acidophilus viable bacteria and the mensuration of mouse laxative function. There are significant differences in the effect of the addition amount on the intestinal probiotic function.

The present invention provides an infant formula milk powder with probiotic function to the intestinal tract, and the milk powder product is composed of the following components by weight:

100-200 parts of basic milk powder, 3-15 parts of prebiotic compound package; preferably 100 parts of basic milk powder: 5 parts of compound package; it is composed of galacto-oligosaccharide and polydextrose in a ratio of 4:1 or 9:1.

The preparation method of milk powder of the present invention comprises the following steps:

Infant formula milk powder preparation process: raw milk inspection→clean milk→cooling, storage→ingredients (adding minerals, heat-stable vitamins, vegetable oil, etc.)→homogenizing→cooling→sterilizing, concentrating→spray drying→dry mixing ingredients (adding prebiotics Compound package and heat unstable vitamins, etc.)→packaging→finished product inspection

(a) The acceptance of raw milk must comply with the requirements stipulated in the national quality standard GB19301-2010 for the purchase of fresh milk, and the sensory inspection, physical and chemical property inspection and microbiological inspection must be strictly carried out. Milk cannot be processed immediately and needs to be stored for a period of time. It must be purified and cooled to 4-6°C by a cooler before being stored in a storage tank.

(b) When batching, the various materials should be mixed in the batching tank according to the requirements of the proportion, and the agitator should be started to mix the materials evenly.

(c) The homogeneous pressure of the mixture is generally controlled at 18MPa.

(d) The high-temperature instantaneous sterilization method can not only kill almost all the microorganisms in the milk, but also make the protein in the milk soft and coagulate, making it easier to digest and absorb after eating.

(e) Vacuum concentration requires the raw milk to be concentrated to 1/4 of the original volume, and the dry matter of the milk reaches about 45%. The concentrated milk temperature is controlled at 47-50°C.

(f) After the emulsion droplets formed by spraying are dried under ideal drying conditions, the diameter can be reduced to 75% of the initial emulsion droplets, the weight can be reduced to 50%, and the volume can be reduced to about 50%.

(g) Dietary fiber and some heat-labile vitamins need to be added after spray drying.

(h) Dried milk powder falls into the bottom of the drying chamber, the temperature of the powder is about 60°C, and the powder should be discharged as soon as possible.

It can also be prepared by other conventional methods in this field.

Beneficial effect:

At present, some prebiotics are usually added to achieve the proliferation of beneficial bacteria, usually including galactooligosaccharides, polydextrose, isomaltooligosaccharides, xylooligosaccharides, fructooligosaccharides, etc. are commonly used. However, which prebiotics or how to compound them will make Bifidobacterium and Lactobacillus acidophilus effectively proliferate and co-exist, which is still a blank. On the basis of a lot of previous work, the applicant creatively mixed galacto-oligosaccharides and polydextrose at a ratio of 4:1 or 9:1 to prepare a compound package for the preparation of formula milk powder, which can effectively regulate the intestinal flora of infants, making Bifidobacterium and Lactobacillus acidophilus compared with only one of them, the number of the two bacteria is significantly increased, resulting in a synergistic symbiosis effect, and at the same time the effect of inhibiting the proliferation of harmful bacteria is the best, so that the intestinal flora of infants can reach balance. Prebiotics can promote the growth of probiotics, and prebiotics have a certain selectivity in promoting probiotics. The present invention uses different prebiotics in a specific ratio, and its beneficial effect on the intestinal tract can be significantly improved, reaching The effect of 1+1>2. Both in vivo and in vitro tests have shown that unpredictable technical effects have been produced.

In addition, adding galactooligosaccharides alone, due to its strong moisture retention, increases the cohesiveness during the milk powder preparation process and affects the instant solubility of milk powder. In this regard, it is found that the combination of polydextrose can solve the above problems, polydextrose is easy to dissolve Water-soluble and slowly fermentable dietary fiber, combined with galacto-oligosaccharide fast and fully fermentable dietary fiber, can slow down the fermentation rate of total dietary fiber and make the pH drop more gently, which protects the intestinal lining and The less stimulation of the anal area improves the baby's tolerance, and most importantly, it also enables the effective proliferation and synergistic symbiosis of Bifidobacteria and Lactobacillus acidophilus, which may be related to the complex molecular structure of polydextrose.

Provided is a formula milk powder with specific composition, specific ratio and specific efficacy.

Detailed ways

Example 1 An infant formula milk powder with probiotic function for the intestinal tract,

After adding the prebiotic compound package components to the milk powder, a milk powder product with the following ratio is formed: the prebiotic compound package is composed of galacto-oligosaccharides and polydextrose in the amount described in Table 1.

Table 1 Composition of formula milk powder

Wherein, the formula (per 100 parts) formula of basic milk powder is as follows:

Experiment 1: Effects of Dietary Fiber Compound Packs in Infant Formula Milk Powder on the Viability of Probiotics

experimental method:

Experimental Example 1: Using the infant formula milk powder of Example 1 as the culture medium, inoculate Bifidobacterium and Lactobacillus acidophilus into the milk powder culture medium respectively, and after anaerobic culture at 37°C for 24 hours, dilute and plate, and culture at 37°C for 48 hours , Colony counting was carried out after colony growth.

Control group 1: using the milk powder of Example 1 as the culture medium, without adding the compound package, and its parts by weight can be replaced by physiological saline.

Control group 2: the milk powder of Example 1 was used as the culture medium, and the compound package was only the same amount of galacto-oligosaccharides.

Control group 3: the milk powder of Example 1 was used as the culture medium, and the compound package was only the same amount of polydextrose.

Control group 4: the milk powder of Example 1 was used as the culture medium, and the compound package was only the same amount of galacto-oligosaccharides, polydextrose and fructo-oligosaccharides, and the ratio of the three was 1:1:1.

The experimental results are shown in Table 2:

Table 2 Effect of dietary fiber compound package in milk powder on the number of viable probiotics

The results in Table 2 show that the addition of oligosaccharides can promote the growth of Bifidobacterium and Lactobacillus acidophilus, and the effect of adding a prebiotic compound package on probiotics is significantly higher than that of the control group with only one prebiotic added. In addition, adding three kinds of prebiotics in the compound package compared with only adding two kinds of prebiotics, 2 kinds of prebiotics is slightly better than 3 kinds, which shows the significant effect of the combination of galacto-oligosaccharide and polydextrose.

Experiment 2: Effect of dietary fiber in infant formula on intestinal flora of mice

Experimental method: The mice were randomly divided into 5 groups, and the mice in the 5 groups were fed with equal amounts of milk powder every day for 14 days, and the number of Bifidobacteria and Lactobacillus acidophilus in the intestinal feces of the mice was detected. The experimental group was gavaged with the infant formula milk powder of Example 1, and the control group 1 was normal saline. Control group 2-4 is the same as the setting of 2-4 in the previous experiment 1, the results are shown in Table 3

Table 3 Effect of dietary fiber in infant formula milk powder on intestinal flora of mice

Experiment 3: The effect of dietary fiber in infant formula on the laxative function of mice

Animal grouping and feeding:

The mice were divided into two batches, the first batch was used for the measurement of small intestinal propulsion, and the second batch was used for the measurement of defecation. Each batch was randomly divided into 7 groups, 7 mice in each group, and they were artificially intervened to achieve the same degree of constipation. They are respectively: low, medium and high dose groups, control group 1 (normal saline group), and control groups 2-4, wherein, the daily feeding amount of the dietary fiber compound package of the mice in the low, medium and high dose groups is respectively 1 The milk powder of weight part/100 weight part, 3 weight part/100 weight part, 5 weight part/100 weight part; And the milk powder with the added amount of dietary fiber composite package being 5 parts by weight/100 parts by weight was all gavaged. All of the above were administered by gastric administration at normal times and in equal amounts every day for 14 consecutive days; the results of each group were averaged.

The small intestine propulsion of the mice, the defecation time of the mice, the number of defecated pellets and the weight of feces were all measured by conventional methods in the art.

The experimental results are shown in the table below:

During the experiment, the shape of the feces of the mice was normal, and no constipation, diarrhea and other adverse symptoms occurred, indicating that the infant formula containing galacto-oligosaccharides and polydextrose had no adverse effects on the growth of mice.

Table A Effects of different dietary fiber additions and types on small intestine propulsion in mice

It can be seen from Table A that there are significant differences in ink propulsion rate after giving mice different dietary fiber compound packaged infant formula milk powder for 14 days. Compared with the model group, the ink propulsion rate of mice in the middle and high dose groups was significantly increased, indicating that the addition amount of galacto-oligosaccharide and polydextrose in infant formula milk powder is 3 parts by weight/100 parts by weight and 5 parts by weight/100 parts by weight It can promote intestinal peristalsis in mice. And when the dietary fiber content in infant formula milk powder is 5 parts by weight/100 parts by weight, the effect is the best, and it will be useless if it is higher. In addition, the promotion effect of probiotics after adding the prebiotic compound package was significantly higher than that of the control group with only one prebiotic added, and the effect of adding three kinds of prebiotics in the compound package was similar to that of the compound package only added two kinds of prebiotics. Ratio, 2 is slightly better than 3.

Table B Effects of different dietary fiber additions and types on the defecation of mice

The effects of different dietary fiber additions and types on the defecation of mice are shown in Table B. After adding the prebiotic compound package, the intestinal function of mice was significantly improved. And when the addition amount of galacto-oligosaccharide and polydextrose in infant formula milk powder is 3 parts by weight/100 parts by weight and 5 parts by weight/100 parts by weight, the laxative ability of mice can be enhanced. When the dietary fiber content in infant formula milk powder is 5 parts by weight/100 parts by weight, that is, the high-dose group has a better ability to improve the laxative effect of mice, which is significantly different from that of the middle-dose group. In addition, the promotion effect of probiotics after adding the prebiotic compound package was significantly higher than that of the control group with only one prebiotic added, and the effect of adding three kinds of prebiotics in the compound package was similar to that of the compound package only added two kinds of prebiotics. Ratio, 2 is slightly better than 3.

It is also found through experiments that the effects of galacto-oligosaccharide and polydextrose at 4:1 and 9:1 are equivalent, and the effect of other ratios is not good. This phenomenon and its reasons need to be further explored in the future.

The above descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope of the present invention.

Claims (5)

1. mixed proportion is 4：1 or 9:1 galactooligosaccharide and the compound packet of polydextrose are being prepared to the raw function of intestinal beneficial Baby formula milk powder in purposes.

2. mixed proportion is 4：1 or 9:The compound packet of 1 galactooligosaccharide and polydextrose simultaneously to Bifidobacterium in enteron aisle and Purposes in live lactobacillus acidophilus number Effective multiplication.

3. the compound packet of Bifidobacterium and live lactobacillus acidophilus number Effective multiplication in a kind of pair of enteron aisle, which is characterized in that by low Poly- galactolipin and polydextrose by weight 4：1 or 9:1 ratio combination.

4. the preparation method that a kind of pair of enteron aisle has the baby formula milk powder of prebiotic function, which is characterized in that by claim Any one of 1-3 compound packet is formed with basic milk powder, wherein basic milk powder 100-200 parts, compound packet is 3- in terms of mass parts 15 parts；Wherein basic milk powder (every 100 parts by weight) is formulated as follows：

5. the baby formula milk powder that a kind of pair of enteron aisle has prebiotic function, which is characterized in that according to claim 4 the method Preparation.