CN108893465A - Magnetic bead is used to show the purposes and impression of the hand trace DNA extraction method of impression of the hand trace - Google Patents
Magnetic bead is used to show the purposes and impression of the hand trace DNA extraction method of impression of the hand trace Download PDFInfo
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- 239000011324 bead Substances 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 57
- 238000007400 DNA extraction Methods 0.000 title abstract description 13
- 241000272814 Anser sp. Species 0.000 claims abstract description 11
- 241000555745 Sciuridae Species 0.000 claims abstract description 11
- 210000003746 feather Anatomy 0.000 claims abstract description 11
- 102000039446 nucleic acids Human genes 0.000 claims description 15
- 108020004707 nucleic acids Proteins 0.000 claims description 15
- 150000007523 nucleic acids Chemical class 0.000 claims description 15
- 238000012546 transfer Methods 0.000 claims description 10
- 238000005336 cracking Methods 0.000 claims description 8
- 238000010828 elution Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 6
- 230000029058 respiratory gaseous exchange Effects 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 abstract description 2
- 230000001680 brushing effect Effects 0.000 abstract description 2
- 241000531807 Psophiidae Species 0.000 description 27
- 239000000284 extract Substances 0.000 description 9
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 9
- 229920000742 Cotton Polymers 0.000 description 8
- 238000010586 diagram Methods 0.000 description 8
- 238000000605 extraction Methods 0.000 description 6
- 239000011148 porous material Substances 0.000 description 6
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- 230000000694 effects Effects 0.000 description 5
- 239000003292 glue Substances 0.000 description 5
- 238000007689 inspection Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 210000004243 sweat Anatomy 0.000 description 3
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000006247 magnetic powder Substances 0.000 description 2
- 230000005415 magnetization Effects 0.000 description 2
- 210000001595 mastoid Anatomy 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
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- 238000001179 sorption measurement Methods 0.000 description 2
- 241001092066 Exochorda Species 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical class ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
- 238000011840 criminal investigation Methods 0.000 description 1
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- 238000005286 illumination Methods 0.000 description 1
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- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
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- 238000000638 solvent extraction Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/117—Identification of persons
- A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
- A61B5/1172—Identification of persons based on the shapes or appearances of their bodies or parts thereof using fingerprinting
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Abstract
The invention belongs to field of biotechnology, disclose purposes and impression of the hand trace DNA extraction method that a kind of magnetic bead is used to show impression of the hand trace, the existing spot impression of the hand showing trace problem, present invention podwer gun or squirrel fingerprint brush or goose feather brush is solved to carry out magnetic bead to spot to brush the mark mark in one's hands that seems.The present invention compared with the prior art in method of breathing out, can be avoided the contaminated problem of biological evidence, can be suitable for use light distribution method（Polarisation, direct light, light transmission etc. carry out light distribution with strong light, laser or other band of light and search impression of the hand trace）Or the occasion that fumigating system is not available.Common brush shows method in compared with the prior art, can obtain more impression of the hand trace evidences, and obtain biological evidence to detect STR parting, and wiping class impression of the hand can not be obtained the technical problem of STR parting by solving the common method of showing of brushing.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to a magnetic bead is used to show the purposes and impression of the hand trace of impression of the hand trace
Mark DNA extraction method.
Background technique
Currently, recognizing during the detection of case because material evidence is same other than the evidences at the scene such as the electronics of modernization, acoustic image
Fixed needs are more focused in traditional criminal scene survey processes by huge finger print information storehouse and DNA information library
The discovery and extraction of biological DNA material evidence and fingerprint trace material evidence.In addition to exhausted small part visual trace can directly by light distribution or
It is outer that photograph extraction fingers and palms line can extract biology DNA identification using cotton swab transfer again（Such as hands wet with perspiration print on apparent blood fingerprint and glass
Trace etc.）, influenced by other many objective condition, many scene impression of the hand traces naked eyes are not visible, can only selective staining and smoked aobvious
Etc. modes shown, but such mode can pollute and destroy cellular nucleic acid, and then influence DNA identification, then cannot again into
Row live biometric DNA sample extracts, once the hands wet with perspiration mark mark shown can not extract fingers and palms line, then by all that has been achieved is spoiled, misses
The source of evidence of two clues to solve the case.
The prior art also has using method of breathing out, and directly surveys personnel by scene and Suspected Area is breathed out and can be shown,
But not only the phenomenon time is of short duration for this method, but also the biological material that may pollute trace extracts.
In criminal scene survey processes, under the premise of not being available light source discovery impression of the hand, using significant powder brush impression of the hand
Trace is common conveniently method, but after utilizing significant powder brush impression of the hand trace, is shifted with cotton swab and extract biology inspection
Material inspection, the biological material of inspection can hardly detect STR parting, so there is many aphalangias significant powder brush in identifying
The impression of the hand trace of line appraisal value（Shape hands wet with perspiration print is drawn as wiped）It can only also select to give up afterwards.
Currently, in addition to common light source direct projection extraction method,《China Criminal Police College's journal 02 phase in 2014》In《502 glue are aobvious
The influence that cast-off cells DNA is extracted is studied after existing fingerprint》With criminal investigation department of Haikou municipal public security bureau about《Contact sample 502
The smoked aobvious rear impression of the hand cast-off cells DNA of glue is extracted》Etc. mention in research achievements, using 502 glue opponent's mark marks carry out it is smoked it is aobvious after use
The method that common law and acetone wipe method carry out transfer extraction purification impression of the hand cast-off cells DNA, test result are shown：Use acetone
It is 1000 ~ 4000RFU that wipe method, which shifts 502 glue and smokes aobvious impression of the hand cast-off cells STR peak ranges, and effect is obvious, and 502 glue are not
The DNA content and detection of sample are had a significant impact.
And in actual operation, many scenes of a crime are because the position that suspect contacts often is the large-sized objects such as wardrobe, door,
Do not have using the cabinet type condition smoked and shown, meanwhile, portable smoked aobvious rifle effect used at present is also bad, and because of 502 smoked aobvious sweat
Impression of the hand is reflected as white trace, and reaction effect is also unobvious on the object of many white backgrounds.So the above advanced method
Also it can only selectively use, not have blanket condition.
Summary of the invention
In order to solve the above-mentioned technical problem the present invention, and provides purposes and hand that a kind of magnetic bead is used to show impression of the hand trace
Mark mark DNA extraction method, can be suitable for various places impression of the hand showing trace extract, have the characteristics that it is applied widely,
The potential hands wet with perspiration mark mark in indetectable scene can be shown, and effectively extract palmmprint and biology DNA and identified, opened up
A possibility that width is solved a case channel, is increased the biological recovery rate of live impression of the hand about 50%, is increased live DNA case-solving rate.
In order to solve the technical problem, the technical scheme adopted by the invention is that：
The present invention provides a kind of purposes：
Magnetic bead is used to show the purposes of impression of the hand trace.
The partial size of the magnetic bead is between 1 μm -5.5 μm.
The partial size of the magnetic bead is 1 μm or 5.5 μm.
The present invention also provides a kind of methods that magnetic bead is used to show impression of the hand trace, which is characterized in that includes the following steps：With
Podwer gun or squirrel fingerprint brush or goose feather brush carry out magnetic bead to spot to brush the mark mark in one's hands that seems.
The partial size of the magnetic bead is between 1 μm -5.5 μm.
The partial size of the magnetic bead is 1 μm or 5 μm.
The present invention also provides a kind of magnetic beads to show the method that DNA is extracted in the transfer of impression of the hand trace, includes the following steps：
（1）, using podwer gun or squirrel fingerprint brush or goose feather brush magnetic bead brushes spot with biological swabs wiping it is aobvious
Obtained impression of the hand trace；
（2）, by step（1）The biological evidence of acquisition carries out cell cracking；
（3）, the cell of cracking is washed；
（4）, elution nucleic acid processing is carried out after washing；
（5）, elution nucleic acid after purification of nucleic acid obtain biological evidence DNA.
Before carrying out wiping impression of the hand trace using biological swabs, taken pictures using camera is aobvious to brush with mark mark.
Compared with prior art, the invention has the advantages that：
Magnetic bead brush is directly obtained hand in spot using by powder blower or squirrel fingerprint brush or goose feather brush by the present invention
Mark mark, takes full advantage of that magnetic bead partial size is small, stability is strong, with magnetic field movement, removes magnetic field, the intensity of magnetization is restored to zero, has
The features such as helping high-efficient washing, and be a magnetic core inside magnetic bead, outside is a layer active group wrapping layer, can with cell,
Protein, nucleic acid, enzyme etc. are coupled, have DNA strong adsorption, and it is nontoxic the features such as.In compared with the prior art
Method of breathing out, can be avoided the contaminated problem of biological evidence, can be suitable for use light distribution method（Polarisation, direct light, light transmission etc.
Light distribution, which is carried out, with strong light, laser or other band of light searches impression of the hand trace）Or the occasion that fumigating system is not available.Compared to
Common brush shows method in the prior art, can obtain more impression of the hand trace evidences, and obtain biological evidence to detect STR
Parting, wiping class impression of the hand can not be obtained the technical problem of STR parting by solving the common method of showing of brushing.
The method middle DNA extraction compared with the prior art that DNA is extracted in the transfer of impression of the hand trace is shown using magnetic bead of the invention
The step of magnetic bead carries out magnetic bead combination is added due to reducing in the solid-phase extraction method of method, i other words solid phase extractions in the prior art
Method successively includes cell cracking, magnetic bead combination, washing cell, elution nucleic acid and purification of nucleic acid step, reduces magnetic bead combination
Step strives brokenly for case and races against time to improve the efficiency of biological evidence DNA extraction.
Detailed description of the invention
Fig. 1 is the impression of the hand trace schematic diagram through light distribution, on sample（Impression of the hand trace is invisible）；
Fig. 2 is the impression of the hand trace schematic diagram without any processing（Impression of the hand trace is invisible）；
Fig. 3 is 1 trumpeter print, 2 trumpeters print and 3 trumpeters print treated impression of the hand trace figure；
Fig. 4 is the enlarged drawing schematic diagram of 1 trumpeter's mark mark figure, it can be seen that there is fingerprint trace to identify condition；
Fig. 5 is the enlarged drawing schematic diagram of 2 trumpeter's mark mark figures, it can be seen that there is fingerprint trace to identify condition；
Fig. 6-Fig. 9 is the DNA lab diagram that 1 trumpeter mark mark 1-4 trumpeter prints that wiping cotton swab carries out DNA identification, can from Fig. 6-Fig. 9
To find out, the 1-4 trumpeter of 1 trumpeter's mark mark prints wiping cotton swab and is capable of detecting when effective DNA typing；
Figure 10-Figure 13 is the DNA lab diagram that 2 trumpeter mark mark 1-4 trumpeters print that wiping cotton swab carries out DNA identification, from Figure 10-Figure 13
In as can be seen that 2 trumpeter's mark marks 1-4 trumpeter print wiping cotton swab be capable of detecting when effective DNA typing.
Specific embodiment
The present invention will be further described with reference to the examples below, and described embodiment is only present invention a part
Embodiment is not whole embodiment.Based on the embodiments of the present invention, those skilled in the art are not making
Other embodiments used obtained under the premise of the labour of creation type, belong to protection scope of the present invention.
The present invention provides a kind of purposes：Magnetic bead is used to show the purposes of impression of the hand trace.
The partial size of the magnetic bead is between 1 μm -5.5 μm.
The partial size of the magnetic bead is 1 μm or 5.5 μm.
The magnetic bead particle size range for biological reagent to circulate currently on the market up between 100nm-5.5 μm, the mankind's
Palm surface is the texturing systems being collectively formed by sweat gland, sudoriferous duct, mastoid process streakline and pore, and sweat can pass through mastoid process line
On recess pore to body surface secrete, adult pore size diameter is between 50mm-250mm, and pore distance is in 0.2mm-
Between 1.0mm etc., about 300 pores on 1 square centimeter[1].When palm contacts object, the sebum sweat that adheres on crestal line
Liquid will be transferred to object surface, form hands wet with perspiration print, at the same time, biological epidermis cast-off cells are attached with sebum in object
On.
Therefore, in order to combine, magnetic bead is manufactured and human body pore be combined with each other to reach optimal and show state, the grain of magnetic bead
Diameter selects 1 μm and 5 μm.
In actual use, magnetic bead partial size is different, and color difference is unobvious, can ignore the influence of magnetic bead color.
The present invention also provides a kind of methods that magnetic bead is used to show impression of the hand trace, include the following steps：With podwer gun or
Squirrel fingerprint brush or goose feather brush carry out magnetic bead to spot to brush the mark mark in one's hands that seems.
The partial size of the magnetic bead is between 1 μm -5.5 μm.
The partial size of the magnetic bead is 1 μm or 5 μm.
The present invention directly obtains magnetic bead brush in spot using by powder blower or squirrel fingerprint brush or goose feather brush
It goes smoothly mark mark, takes full advantage of that magnetic bead partial size is small, stability is strong, with magnetic field movement, remove magnetic field, the intensity of magnetization is restored to
Zero, the features such as facilitating high-efficient washing, and be a magnetic core inside magnetic bead, outside is a layer active group wrapping layer, Ke Yihe
Cell, protein, nucleic acid, enzyme etc. are coupled, have DNA strong adsorption, and it is nontoxic the features such as.Compared to existing skill
Method of breathing out in art can be avoided the contaminated problem of biological evidence, and can be suitable for can not using irradiation or fumigating system
The occasion used.Common brush shows method in compared with the prior art, can obtain more impression of the hand trace evidences, and will not be dirty
Contaminate biological evidence.
The present invention also provides a kind of magnetic beads to show the method that DNA is extracted in the transfer of impression of the hand trace, includes the following steps：
（1）, using podwer gun or squirrel fingerprint brush or goose feather brush magnetic bead brushes spot with biological swabs wiping it is aobvious
Obtained impression of the hand trace；
（2）, by step（1）The biological evidence of acquisition carries out cell cracking；
（3）, the cell of cracking is washed；
（4）, elution nucleic acid processing is carried out after washing；
（5）, elution nucleic acid after purification of nucleic acid obtain biological evidence DNA.
Before carrying out wiping impression of the hand trace using biological swabs, taken pictures using camera is aobvious to brush with mark mark.
Herein it should be noted that the magnetic bead that the present invention is told about is dry solid powder magnetic bead.
The method middle DNA extraction compared with the prior art that DNA is extracted in the transfer of impression of the hand trace is shown using magnetic bead of the invention
The step of magnetic bead carries out magnetic bead combination is added due to reducing in the solid-phase extraction method of method, i other words solid phase extractions in the prior art
Method successively includes cell cracking, magnetic bead combination, washing cell, elution nucleic acid and purification of nucleic acid step, reduces magnetic bead combination
Step strives brokenly for case and races against time to improve the efficiency of biological evidence DNA extraction.
Solid-phase extraction method is one of DNA extraction method, and each step of solid-phase extraction method belongs to the prior art, this field
Technical staff can be appreciated and understood that details are not described herein.The DNA extraction method of use further includes organic solvent extraction, such as
Saturated phenol-chloroform extraction；Salting out method mainly dissolves to extract according to different concentration to DNA ingredient（DNA exists
Solubility is minimum in the sodium chloride solution of 0.14mol/L）；Chelex-100 extraction method is copolymerized using styrene, divinylbenzene
Body extracts, and is common minim DNA extraction method.And fixed extraction method is common automation platform operating method, is commonly used
Reagent be the particles such as bead, silica bead, magnetic bead.Because " DNA solid-phase extraction method " unique easy to operate, DNA recovery rate is high, is convenient for
The features such as automatic operation, therefore " DNA solid-phase extraction method " is also exactly that DNA extraction is the most universal in current material evidence appraisal organization
Method, and the present invention is to reduce magnetic bead combination the step in existing solid-phase extraction method, those skilled in the art is
It can be appreciated that and understand, details are not described herein.
In order to prove that magnetic bead of the present invention for showing the effect of impression of the hand trace, does following experiment.
1,4 regions are identified on desk edge strip respectively with marking pen, sticking scale and compiling respectively is No. 1-4；
2, it is printed respectively by wiping oarsman at upper 4 pieces of fingerprints and 4 in the same way with hand in the region edge strip 1-4,1,2 and 3, No. 4
Between time difference be 5 hours, number be 1-4 trumpeter print, after 1 hour carry out impression of the hand experiment；
3, observation and with impression of the hand trace in the region 1-4 on light intensity light source two kinds of objects of multiple angular illuminations, as shown in Figure 1,
Impression of the hand trace is invisible.
4, to the processing of desk edge strip：1 trumpeter print is shown with 1mm magnetic bead brush respectively after 1 hour, 5mm magnetic is used after 5 hours
Pearl brush shows 2 trumpeters print, alternately brushes aobvious partial region with squirrel fingerprint brush and goose feather brush, i other words, 1 trumpeter print squirrel fingerprint
Brush, 2 trumpeters print then with goose feather brush；3 trumpeters print is shown with black magnetic powder brush after 1 hour, wherein treated 1 trumpeter print, two trumpeters
The impression of the hand trace of print and three trumpeters print is as shown in Figure 3.As a comparison, 4 trumpeters print is without any processing, as shown in Fig. 2, No. 4 areas
Domain impression of the hand trace is invisible.In such a way that Fig. 1 to Fig. 5 can be seen that this using the aobvious impression of the hand trace of magnetic bead brush, existing skill is compared
Art brush shows method, and effect is more excellent, and aobvious method is commonly brushed especially for the prior art（Magnetic powder）DNA can not be carried out by wiping a stroke class fingerprint
The problem of extracting identification shows method by magnetic bead brush, can satisfy showing and extracting for such fingerprint.
5, carry out taking pictures it is fixed after compare, shift on edge strips 1,2 trumpeter's mark marks simultaneously with 2 groups of biological swabs wipings respectively
Compile is that 1-4 trumpeter prints the standby inspection of wiping cotton swab.
6, above-mentioned cotton swab inspection is subjected to DNA identification, and extracts identification by DNA, it can from Fig. 6-Figure 13
Out, wherein the peak value in Fig. 6-Figure 13 is DNA peak value, represents numerical value below DNA peak value, and peak value is higher, and DNA identification is stronger,
Wherein, for the DNA lab diagram in Fig. 6-Figure 13, those skilled in the art can be appreciated and understood that details are not described herein.From
Can intuitively it find out in DNA lab diagram, 1 trumpeter print and 2 trumpeters print can detect effective dna parting, it was demonstrated that magnetic bead brush shows hand
Mark mark has identification condition.
Claims (10)
1. the purposes that magnetic bead is used to show impression of the hand trace.
2. purposes according to claim 1, which is characterized in that the partial size of the magnetic bead is between 1 μm -5.5 μm.
3. purposes according to claim 1, which is characterized in that the partial size of the magnetic bead is 1 μm or 5.5 μm.
4. a kind of method that magnetic bead is used to show impression of the hand trace, which is characterized in that include the following steps：With podwer gun or squirrel
Fingerprint brush or goose feather brush carry out magnetic bead to spot to brush the mark mark in one's hands that seems.
5. the method that magnetic bead according to claim 4 is used to show impression of the hand trace, which is characterized in that the partial size of the magnetic bead
Between 1 μm -5.5 μm.
6. the method that magnetic bead according to claim 4 or 5 is used to show impression of the hand trace, which is characterized in that the magnetic bead
Partial size is 1 μm or 5 μm.
7. a kind of magnetic bead shows the method that DNA is extracted in the transfer of impression of the hand trace, which is characterized in that include the following steps：
（1）, using podwer gun or squirrel fingerprint brush or goose feather brush magnetic bead brushes spot with biological swabs wiping it is aobvious
Obtained impression of the hand trace；
（2）, by step（1）The biological evidence of acquisition carries out cell cracking；
（3）, the cell of cracking is washed；
（4）, carry out elution nucleic acid after washing；
（5）, purification of nucleic acid obtain biological evidence DNA.
8. magnetic bead according to claim 7 shows the method that DNA is extracted in the transfer of impression of the hand trace, which is characterized in that step（1）
Used in magnetic bead partial size be 1-5.5 μm.
9. magnetic bead according to claim 7 shows the method that DNA is extracted in the transfer of impression of the hand trace, which is characterized in that step（1）
Used in magnetic bead partial size be 1 μm or 5 μm.
10. magnetic bead according to claim 7 shows the method that DNA is extracted in the transfer of impression of the hand trace, which is characterized in that carrying out
Step（1）Before, it is taken pictures to what magnetic bead brush was shown with mark mark using camera.
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| CN102229927A (en) * | 2011-06-03 | 2011-11-02 | 武汉哇哇噻纳技术开发有限公司 | Method and reagent for improving rate of extraction of DNAs of castoff cells in case trace sample |
| CN103622701A (en) * | 2012-08-23 | 2014-03-12 | 武汉哇哇噻纳技术开发有限公司 | Method for showing fingerprints through silanization nanometer magnetic powder |
| CN105462961A (en) * | 2016-01-14 | 2016-04-06 | 苏州新海生物科技股份有限公司 | Nucleic acid extraction method and reagent kit |
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