CN108785691A - A kind of artificial tubercle and localization method carrying out detection and localization to minimal disease - Google Patents

Abstract

A kind of artificial nodules for localization detection of tiny lesions. The artificial nodules are formed by solidification of a chemical mixture. They are hardened nodules formed next to or surrounding tiny lesions, and are clearly visible in CT tomography. nodules. By injecting the artificial nodule liquid mixture in the designated micro lesion area, a sclerotic nodule is formed next to or surrounding the micro lesion, and the artificial nodule is clearly visible in the CT tomographic scan. Precise localization of the lesion.

Description

An artificial nodule and positioning method for positioning and detecting tiny lesions

technical field

The invention relates to a method for locating tiny tumor lesions, in particular to a method for locating tiny tumor lesions by using artificial nodules and the artificial nodules, which are mainly used for detecting and locating tiny lesions. It belongs to the technical field of medical detection.

Background technique

Tumor is one of the major diseases threatening human health today. Early detection and early treatment are current research hotspots. However, for various reasons, most patients are still diagnosed at an advanced stage. The tumor metastasizes, and the patient suffers from an incurable disease. According to reports, more than 4 million new tumors are newly discovered in my country every year, and 2.8 million people die. About 70% of the tumors are malignant tumors originating from the epithelium, which are called cancers in medicine. Most people have a fear of cancer.

With the extensive development of CT scans, many tiny lesions were found. When these small lesions are difficult to characterize or suspected to be malignant lesions, lesion resection is required to obtain a definite diagnosis and achieve the purpose of early diagnosis and treatment of the disease. Due to various reasons such as the growth location, size, and hardness of these lesions, intraoperative positioning is difficult, which affects accurate resection. Accurate localization of these lesions will facilitate accurate surgical resection.

Currently, there are various methods for locating small lesions. Mainly include: intraoperative naked eye observation, surgical instrument surface sliding positioning, intraoperative finger palpation positioning, intraoperative thoracoscopic B-ultrasound probe positioning, intraoperative CT positioning, and preoperative CT guided positioning. Including: methylene blue injection, percutaneous microcoil placement, percutaneous Hookwire positioning, radiotracer injection, and image-assisted navigation. However, these methods all have complex detection methods and high operational difficulties, resulting in complicated and costly methods for locating tiny lesions. Because there is currently a lack of a simple, reliable, and versatile method for locating small lesions, research on new methods for locating small lesions is the key to precise surgical resection of small lesions.

No patent literature reports on the same technology as the present invention have been found through patent retrieval, and the patents that have a certain relationship with the present invention mainly include the following:

1. The patent number is CN201210022812.7, and the invention patent titled "Cell sheet for tissue repair and bioartificial tissue engineering and its preparation method" discloses a cell for tissue repair and bioartificial tissue engineering piece. The cell sheet contains treated stem cells that are embedded in their autocrine extracellular matrix (ECM) and form a cell sheet. The cell sheet is formed by isolating stem cells, expanding the stem cells, and treating the stem cells with a biological factor or a factor that causes the production of a biological factor, thereby inducing differentiation of the stem cells in vitro, producing an extracellular matrix, and forming a cell sheet. The cell sheets are used as bioactive or cell-free materials to promote tissue repair, or to form bioartificial organs for tissue replacement. The cell sheets of the present invention do not require the use of scaffolds to deliver cells. The cell sheet facilitates cell engraftment in vivo and provides tensile mechanical strength for withstanding early mechanical loads during tissue repair.

2. The patent application number is CN200410045121.4, and the title is "branched synthetic peptide immunogen construct with artificial T helper cell epitope coupled to B cell epitope" invention patent, which discloses a variety of Different types of novel non-linear, branched synthetic peptide immunogen constructs. Each construct uses a different artificial Th epitope. The Th epitope is linked to the B epitope in the form of MAP, which is used to induce antibodies targeting the B epitope to which it is linked. These novel peptide immunogen constructs elicit high titers of antibodies specifically targeting the B epitope and produce measurable biological efficacy results. The composition of the present invention can be used for the preparation of vaccines to produce immune protection against infectious diseases, or for immunotherapy to treat diseases caused by disorders of normal physiological processes, or for immunotherapy to treat cancer, as well as to intervene and modify normal physiological processes. process.

3. The patent application number is CN02807665.6, and the invention patent titled "CD8α+ lymphoid dendritic cells differentiated from human hematopoietic stem cells and differentiation method" discloses a CD8α+ immunophenotype of lymphoid dendritic cells, It is differentiated from human hematopoietic stem cells. In another aspect, there is provided a method for differentiating lymphoid dendritic cells, the method comprising culturing human hematopoietic stem cells in two steps, the first step being in a first medium containing GM-CSF, followed by culturing in a medium containing IFN-γ in the second medium. In another aspect, a pharmaceutical composition for immunotherapy is provided, which includes a therapeutically effective amount of dendritic cells. In another aspect, there is provided a method of treating an immune-related disease in a mammal comprising administering lymphoid dendritic cells to a subject in need thereof in an amount effective to treat the disease.

4. The patent number is CN201210009890.3, and the invention patent titled "CREPT antibody for identifying tumor cells or tumor tissues" discloses a CREPT antibody for identifying tumor cells or tumor tissues. Provided is the application of the reagent for detecting CREPT protein in the following (a) or (b) or (c): (a) assisting in identifying tumor cells; (b) assisting in identifying tumor tissues; (c) assisting in identifying tumor patients. The present invention finds that the expression level of CREPT gene in tumor cell line is much higher than that in normal cells, and the expression level in tumor tissue is much higher than that in paracancerous tissue and normal tissue.

Although some of the above-mentioned patents involve the identification and analysis of tumor cells or tumor tissues, some involve artificial auxiliary cells, and some improved technical solutions have been proposed, but after careful reading, it is found that these patents do not involve the positioning of small lesions. , and it is not feasible to directly use these detection or identification methods to locate small lesions, so how to accurately locate small lesions remains to be further studied.

Invention content:

The purpose of the present invention is to propose a new method for locating tiny lesions in view of the existing problems in locating tiny lesions. This new method for locating tiny lesions can perform simple, reliable and versatile position.

In order to achieve this goal, the technical embodiment of the present invention is to provide an artificial nodule for positioning detection of tiny lesions. The artificial nodule is formed by solidification of a chemical mixture, and the hardening formed next to or surrounding the tiny lesions Nodules, and are artificial nodules that are clearly visible on CT tomography.

Further, the chemical mixture is formed by mixing two or more compounds;

Further, the compound is a liquid or powder injection compound, which is mixed into a liquid injection, and after being injected into the designated micro lesion area, nodules are rapidly formed next to or surrounding the micro lesion.

Further, the compounds include various liquid or powder injection medical polymer materials, including various non-toxic monomers or polymers, such as methylchlorosilane, phenylchlorosilane, cyanoacrylate compounds, α-octyl cyanoacrylate, polyethylene, and amino acid polymers as catalysts, including acidic polyamino acids, polyamino acids, amino acid copolymers, such as 2-cyano-2-butyl acrylate.

Further, the chemical mixture contains an enhanced contrast agent for CT scan, and the enhanced contrast agent for CT scan includes an iodine-containing organic compound.

Further, the chemical mixture contains a color enhancer, and when the color enhancer is added to the chemical mixture to facilitate the localization of tiny lesions, the position of artificial nodules can be more clearly perceived.

A method for locating tiny lesions using the above-mentioned artificial nodules, by injecting a liquid mixture of artificial nodules in the designated area of the tiny lesions to form a sclerotic nodule next to or surrounding the tiny lesions, and in a CT tomographic scan Clearly visible artificial nodules, precise positioning of tiny lesions based on CT tomographic positioning data.

Further, the injection of the artificial nodule liquid mixture in the designated micro lesion area is to inject the artificial nodule liquid mixture in the found micro lesion area after routine testing finds the micro lesion area, and the artificial nodule liquid mixture is in the vicinity of the micro lesion area. A sclerotic nodule forms next to or surrounding tiny lesions.

Further, the artificial nodules that are clearly visible in CT tomography are artificial nodules that are artificially scanned by CT tomography after the liquid mixture of artificial nodules forms a hardened nodule next to or surrounding the tiny lesions.

Further, the precise positioning of the tiny lesions according to the positioning data of the CT tomography is to judge the precise position of the tiny lesions by observing the microscopic coordinates of the artificial nodules after the artificial nodules are scanned by the CT tomography.

Further, the artificial nodules include the following steps:

1) Perform a low-dose CT scan on the designated area to determine the basic projection points of the small lesions and the small lesions on the body surface, and calculate the depth and angle of the needle;

2) Select artificial nodule liquid mixture;

3) Inject the artificial nodule liquid mixture into the designated area, and let the artificial nodule liquid mixture form a hardened nodule, which is clearly visible in the CT scan;

4) Use CT tomography to find artificial nodules in the designated area;

5) According to the results of CT tomography, coordinate data is used to determine the position of artificial nodules, so as to locate the precise position of tiny lesions.

The advantages of the present invention are:

The present invention sets artificial nodules at tiny lesions and utilizes artificial nodules to locate the precise position of tiny lesions, which has the following advantages:

1) Use artificial nodules to accurately locate the precise location of tiny lesions, which can accurately determine the location of tiny lesions and facilitate positioning detection;

2) The localization and detection method of this kind of tiny lesions is easy to implement. After the artificial nodule liquid mixture is prepared, it is injected into the designated area, and the detection can be carried out;

3) The artificial nodules are easy to manufacture, low in cost, wide in versatility, and easy to popularize and adopt.

Detailed ways:

The present invention will be described in further detail below in conjunction with embodiment. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments; it can be understood that the specific embodiments described here are only used to explain the present invention, rather than limit the present invention, Any other embodiments obtained by persons of ordinary skill in the art based on the principle of the present invention without creative efforts fall within the protection scope of the present invention.

Embodiment one

A kind of artificial nodules for localization detection of tiny lesions. The artificial nodules are formed by solidification of a chemical mixture. They are hardened nodules formed next to or surrounding tiny lesions, and are clearly visible in CT tomography. nodules.

The chemical mixture is formed by mixing two compounds;

The compound is a liquid or powder injection compound, which is mixed into a liquid injection, and after being injected into a designated area of a small lesion, nodules are rapidly formed next to or surrounding the small lesion.

The compound includes various liquid or powder injection medical polymer materials. The polymer material is a mixture of cyanoacrylate compounds and α-octyl cyanoacrylate. The compound is used as a catalyst; the addition amount of the catalyst is 1-3% by weight of the mixture of cyanoacrylate compound and α-octyl cyanoacrylate.

The chemical mixture contains an enhanced contrast agent for CT scanning, and the enhanced contrast agent for CT scanning is an iodine-containing organic compound; the added amount of the iodine-containing organic compound is 1-2% of the total mass of the chemical mixture.

The chemical mixture contains a color enhancer, which is methylene blue; the amount of the methylene blue added is 1-2% of the total mass of the chemical mixture. When a color enhancer is added to the chemical mixture to facilitate the localization of small lesions, the position of artificial nodules can be more clearly perceived.

A method for locating tiny lesions using the above-mentioned artificial nodules, by injecting a liquid mixture of artificial nodules in the designated area of the tiny lesions to form a sclerotic nodule next to or surrounding the tiny lesions, and in a CT tomographic scan Clearly visible artificial nodules, precise positioning of tiny lesions based on CT tomographic positioning data.

The injection of the artificial nodule liquid mixture in the designated small lesion area is to inject the artificial nodule liquid mixture in the small lesion area found after the routine detection of the small lesion area, and the artificial nodule liquid mixture is placed next to the small lesion area or A sclerotic nodule surrounded by tiny lesions.

The artificial nodules that are clearly visible in CT tomographic scanning are artificial nodules that are scanned by CT tomographic scanning after the liquid mixture of artificial nodules forms a sclerotic nodule adjacent to or surrounding tiny lesions.

The precise positioning of the tiny lesions according to the positioning data of the CT tomography is to judge the precise position of the tiny lesions by observing the microscopic coordinates of the artificial nodules after the artificial nodules are scanned by the CT tomography.

Described artificial nodule comprises the following steps:

1) Perform a low-dose CT scan on the designated area to determine the basic projection points of the small lesions and the small lesions on the body surface, and calculate the depth and angle of the needle;

2) Select artificial nodule liquid mixture;

3) Inject the artificial nodule liquid mixture into the designated area, and let the artificial nodule liquid mixture form a hardened nodule, which is clearly visible in the CT scan;

4) Use CT tomography to find artificial nodules in the designated area;

5) According to the results of CT tomography, coordinate data is used to determine the position of artificial nodules, so as to locate the precise position of tiny lesions.

Embodiment two

The principle of medical ethics in Example 2 is the same as that in Example 2, except that the composition of the selected compound to form artificial nodules is different. It is an artificial pulmonary nodule for positioning and detecting tiny lesions. A hardened nodule formed next to or surrounding tiny lesions, formed by solidification of chemical mixture, is an artificial pulmonary nodule clearly visible on CT tomography.

The chemical mixture is formed by mixing three compounds;

The compound is a mixture of a liquid compound and a powder injection compound, which is a liquid injection after being mixed. After being injected into a designated micro lesion area, nodules are rapidly formed next to or surrounding the micro lesion.

The compound is cyanoacrylate compound, α-octyl cyanoacrylate and methylchlorosilane in any proportion; and a linear polymer of amino acid is used as a catalyst. Further, the chemical mixture contains an iodine-containing organic compound CT scan enhancement contrast agent.

Further, the chemical mixture contains a fluorescent color enhancer, and when the color enhancer is added to the chemical mixture to facilitate the localization of small lesions, the position of the artificial pulmonary nodule can be more clearly perceived.

A method for locating tiny lesions using the aforementioned artificial pulmonary nodules, by injecting a liquid mixture of artificial lung nodules in the designated area of the tiny lesions to form a sclerotic nodule next to or surrounding the tiny lesions, and showing the results on CT tomography The artificial lung nodules that are clearly visible in the scan can be precisely positioned for tiny lesions based on the positioning data of CT tomography.

The injection of the artificial pulmonary nodule liquid mixture in the designated small lesion area is to inject the artificial pulmonary nodule liquid mixture in the small lesion area found after the routine detection of the small lesion area, and the artificial pulmonary nodule liquid mixture is placed next to the small lesion area. A sclerotic nodule forms next to or surrounding tiny lesions.

The artificial pulmonary nodules that are clearly visible in CT tomographic scanning are artificial pulmonary nodules that are scanned by CT tomographic scanning after the liquid mixture of artificial nodules forms a sclerotic nodule adjacent to or surrounding tiny lesions.

The precise positioning of the tiny lesions according to the CT tomographic positioning data is to judge the precise position of the tiny lesions by observing the microscopic coordinates of the artificial pulmonary nodules after the CT tomographic scanning of the artificial pulmonary nodules.

Described artificial pulmonary nodule localization comprises the following steps:

1) Perform low-dose scanning on the designated area to determine the small lesions and the basic projection points of the small lesions on the body surface, and calculate the depth and angle of the needle;

2) Select cyanoacrylate compounds, α-octyl cyanoacrylate and methylchlorosilane as the liquid mixture of artificial lung nodules; and add iodine-containing organic compounds and fluorescent color enhancers in the liquid mixture; various ingredients A total of 0.4ml is evenly mixed together;

3) Aspirate the artificial pulmonary nodule liquid mixture with 1ml of injection, inject 0.3ml of the artificial pulmonary nodule liquid mixture into the designated area, and let the artificial pulmonary nodule liquid mixture form a hardened nodule, which is in the CT tomographic scan Clearly visible artificial nodules;

4) Start CT scanning 30 seconds after injecting the liquid mixture of artificial pulmonary nodules, and measure the data related to artificial pulmonary nodules and pulmonary microlesions;

5) According to the results of CT tomography, coordinate data is used to determine the position of artificial pulmonary nodules, so as to locate the precise position of tiny lesions.

The advantages of the present invention are:

The present invention sets artificial nodules at tiny lesions and utilizes artificial nodules to locate the precise position of tiny lesions, which has the following advantages:

4) Using artificial nodules to accurately locate the precise position of tiny lesions can accurately determine the position of tiny lesions, which is convenient for positioning detection;

5) The localization and detection method of this kind of tiny lesions is easy to implement. After the artificial nodule liquid mixture is prepared, it is injected into the designated area, and the detection can be carried out;

The artificial nodule is convenient to manufacture, low in cost, wide in versatility, and easy to popularize and adopt.

Claims (10)

1. An artificial nodule for positioning detection of tiny lesions, characterized in that: artificial nodules are formed by solidification of a chemical mixture, and are hardened nodules formed next to or surrounding tiny lesions, and are formed on CT slices. Artificial nodules clearly visible on the scan. 2. The artificial nodule for localization detection of tiny lesions according to claim 1, characterized in that: said chemical mixture is formed by mixing two or more compounds. 3. The artificial nodule for positioning and detecting tiny lesions as claimed in claim 2, characterized in that: the compound is a liquid or a powder injection compound, and after mixing, it becomes a liquid injection, and after being injected into the designated tiny lesions area , rapidly forming nodules adjacent to or surrounding tiny lesions. 4. The artificial nodule for positioning detection of tiny lesions according to claim 3, characterized in that: said compounds include various liquid or powder injection medical polymer materials, including various non-toxic monomers or polymers, and amino acid polymers are used as catalysts. 5. The artificial nodule for positioning detection of tiny lesions according to claim 1, characterized in that: said chemical mixture contains a contrast agent for CT scan enhancement. 6. The artificial nodule for localizing and detecting tiny lesions as claimed in claim 1, characterized in that: said chemical mixture contains a color enhancer, and when the color enhancer is added to the chemical mixture to facilitate the positioning of tiny lesions, more The location of artificial nodules is clearly perceived. 7. A method for locating tiny lesions using the artificial nodules described in claim 1, by injecting the artificial nodule liquid mixture in the designated area of the tiny lesions to form a sclerotic nodule next to the tiny lesions or surrounding the tiny lesions, And the artificial nodules that are clearly visible in CT tomography can be accurately positioned according to the positioning data of CT tomography. 8. The method for locating tiny lesions as claimed in claim 7, characterized in that: the injection of the artificial nodule liquid mixture in the designated tiny lesions is performed after the tiny lesions are found in routine testing. The artificial nodule liquid mixture is injected into the micro lesion area, and the artificial nodule liquid mixture forms a sclerotic nodule next to or surrounding the micro lesion. 9. The method for locating tiny lesions as claimed in claim 8, characterized in that: the artificial nodules clearly visible in the CT tomographic scan are the artificial nodules liquid mixture next to the tiny lesions or surrounding the tiny lesions After a sclerotic nodule is formed, the artificial nodules are scanned by CT tomography; the precise positioning of tiny lesions based on the CT tomographic scanning positioning data is to judge the microscopic coordinates of the artificial nodules by observing the microcoordinates of the artificial nodules after CT tomography scanning. The precise location of the lesion. 10. The method for locating tiny lesions as claimed in claim 7, wherein said artificial nodules comprise the following steps: 1) Select artificial nodule liquid mixture; 2) Inject the artificial nodule liquid mixture into the designated area, and let the artificial nodule liquid mixture form a hardened nodule, which is clearly visible in the CT scan; 3) Use CT tomography to find artificial nodules in the designated area; 4) According to the results of CT tomography, coordinate data is used to determine the position of artificial nodules, so as to locate the precise position of tiny lesions.