[go: up one dir, main page]

CN108778351A - 用于改进罗非鱼皮的方法及其用于覆盖皮肤损伤的用途 - Google Patents

用于改进罗非鱼皮的方法及其用于覆盖皮肤损伤的用途 Download PDF

Info

Publication number
CN108778351A
CN108778351A CN201680051079.8A CN201680051079A CN108778351A CN 108778351 A CN108778351 A CN 108778351A CN 201680051079 A CN201680051079 A CN 201680051079A CN 108778351 A CN108778351 A CN 108778351A
Authority
CN
China
Prior art keywords
skin
solution
sterile
tilapia skin
saline
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201680051079.8A
Other languages
English (en)
Inventor
埃德马尔·马西埃尔·利马·儒尼奥尔
曼诺尔·鄂多立克·德·莫赖斯·菲略
马塞洛·何塞·博格斯·德·米兰达
尼尔森·萨尔托·皮科洛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Edmar Maciel Lima Junior
Energetica Do Ceara-Coelce Cia
Marcelo Jose Borges De Miranda
Saiala Federal University
Original Assignee
Instituto De Apoio Ao Queimado-Iaq
Energetica Do Ceara-Coelce Cia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=58186353&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN108778351(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Instituto De Apoio Ao Queimado-Iaq, Energetica Do Ceara-Coelce Cia filed Critical Instituto De Apoio Ao Queimado-Iaq
Publication of CN108778351A publication Critical patent/CN108778351A/zh
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/124—Disinfecting agents, e.g. antimicrobials
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/44—Medicaments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/42—Use of materials characterised by their function or physical properties
    • A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/04—Materials for stopping bleeding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Materials Engineering (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dermatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

本发明涉及罗非鱼皮的用途，该罗非鱼皮基于在5型和7型洁净室环境中浓度在50‑99％范围的甘油进行若干步骤的处理，并且在某些情况下，当微生物数量高时，有必要通过伽马辐照进行补充辐射消毒。该鱼皮可以用作诸如烧伤和急性或慢性伤口的皮肤损伤的闭合性生物敷料。

Description

用于改进罗非鱼皮的方法及其用于覆盖皮肤损伤的用途
技术领域
本发明涉及罗非鱼皮的用途，该罗非鱼皮基于在5型和7型洁净室环境中浓度在50-99％范围的甘油进行若干步骤的处理，并且在某些情况下，当微生物数量高时，有必要通过伽马辐照进行补充辐射灭菌。该鱼皮可用于烧伤和急性或慢性伤口，包括其在浅层或深层的二度烧伤造成的损伤表面上的应用，在急性伤口例如自体移植的供体皮肤区域或者慢性伤口中的应用，或在麻醉下进行简单外敷、外科清洁或外科清创的最后阶段以及经常出现在这类损伤中的自体植皮中的应用。
背景技术
目前，巴西的医学并不具备用于(动物来源的)临时异源皮肤外敷物的替代选择，以作为烧伤和伤口治疗的一部分。数十年来，在发达国家，尤其是美国，工业化猪皮被大规模地用于这一目的。考虑到高昂的成本和巴西的经济现状，将该产品进口到巴西并不具备商业可行性。
具体实施方式
本发明描述了罗非鱼皮在诸如烧伤、慢性伤口等皮肤损伤的表面处理中的用途，所述罗非鱼获自使用培养系统(tanks-net)的养鱼场，通过灭菌过程，如下所述。
屠宰后，使用绿松石(工具)剥除罗非鱼皮(重量为800g至1000g之间)并将其在活水中进行清洗，以移除任何微量的血液和其它杂质，并将其置于预先冷却至4℃的无菌生理盐水(0.9％NaCl溶液)中进行最终清洗。随后，移除仍贴附于鱼皮上多余的肌肉，并将鱼皮切割成10.0cm×5.0cm的块并用生理盐水清洗，随后通过以下步骤进行灭菌。
第一步：将鱼皮置于包含2％葡萄糖酸氯己定(具有表面活性剂的溶液)的无菌容器中，并在其中保持30分钟。
第二步：在上一步操作后，使用无菌生理盐水清洗鱼皮并将其置于另一个包含另外的2％葡萄糖酸氯己定溶液的容器中，并在其中保持30分钟。
第三步：使用无菌生理盐水冲洗鱼皮并将其置于包含50％甘油、49％生理盐水和1％的具有青霉素、链霉素和fungisol的溶液的容器中，将其包装在含冰的恒温箱中用于运输(Jaguaribara-NPDNI)。
下述步骤在具有水平或垂直层流的无菌环境中在UFC的NPDM中进行。
第四步：24小时之前，应将鱼皮从前述溶液中取出，使用无菌生理盐水清洗，并置于无菌、密封的包含75％甘油、24％生理盐水和1％的具有青霉素、链霉素和fungisol的溶液的密闭容器中，在该容器中，将鱼皮在该溶液中按揉5分钟，并且在具有恒定转速为15转/分钟的搅拌器和温度为37℃的水浴(离心机)中保持3小时。
第五步：将鱼皮取出，再次用无菌生理盐水清洗，并置于另一个无菌且密封的包含99％甘油和1％的具有青霉素、链霉素和fungisol的溶液的容器中，将鱼皮在该溶液中按揉5分钟，并在转速为15转/分钟且温度为37℃的离心机中的水浴中另外保持3小时。
第六步：在使用甘油的上一步结束时，将鱼皮包装于无菌的双层塑料包封中并于4℃下储存以供日后使用，保存限期为最长达两年。
从天然鱼皮开始，即在第一步之前和上述六个步骤之中，对革兰氏阳性细菌、革兰氏阴性细菌和真菌进行七次微生物学测试。当用于细菌计数(即生物负载微生物分析或微生物限度测试)的生物负载水平在可接受范围内时，该鱼皮可供使用，其中所述生物负载微生物分析或微生物限度测试在加工和处理期间对需要控制微生物水平的药物和医疗产品上进行。
当使用鱼皮时，将鱼皮从包封中取出并且在不同的无菌生理盐水中清洗三次，每次清洗五分钟。在此步骤之后，鱼皮被切割成伤口的尺寸并作为封闭敷料使用。
若细菌计数高于10/3，应进行如下所述的补充灭菌步骤：
第七步：补充辐射灭菌，伽马-钴60，其中负荷剂量范围为15至50千戈瑞，取决于生物负载水平(微生物计数)。
通过粘附在创面上，罗非鱼皮在与这些损伤相互作用时会促进真皮基质的愈合和修复过程的加速(由于其组织结构中I型胶原蛋白的作用)，避免渗出液停留和体液流失，增强阻止细菌侵入的屏障，并缓解疼痛。

Claims (6)

1.一种用于改进罗非鱼皮的方法，其特征在于，所述方法通过以下步骤进行：
-使用绿松石剥除罗非鱼皮并在活水中冲洗，置于预先冷却至4℃的无菌生理盐水(0.9％NaCl溶液)中进行最终清洗，随后将多余的肌肉去除，并切割成10.0cm×5.0cm的块，用生理盐水清洗，随后按照以下步骤进行灭菌：
-将所述罗非鱼皮置于包含2％葡萄糖酸氯己定(具有表面活性剂的溶液)的无菌容器中，并在其中保持30分钟；
-使用无菌生理盐水清洗所述罗非鱼皮并将所述罗非鱼皮置于另一个包含另外的2％葡萄糖酸氯己定溶液的容器中，并在其中保持30分钟；
-使用无菌生理盐水溶液冲洗所述罗非鱼皮，并将所述罗非鱼皮置于包含50％甘油、49％生理盐水和1％的具有青霉素、链霉素和fungisol的溶液的容器中。
2.根据权利要求1所述的方法，其特征在于，下述步骤在具有水平或垂直层流的无菌环境中进行。
3.根据权利要求1或2所述的方法，其特征在于以下步骤：
-24小时之前，将所述罗非鱼皮从所述溶液中取出，使用无菌生理盐水清洗，并置于无菌且密封的包含75％甘油、24％生理盐水和1％的具有青霉素、链霉素和fungisol的溶液的容器中(在所述溶液中按揉所述罗非鱼皮5分钟)，并且在具有恒定转速为15转/分钟的搅拌器和温度为37℃的水浴(离心机)中保持3小时；
-将所述罗非鱼皮取出，再次用无菌生理盐水清洗，并置于另一个无菌且密封的包含99％甘油和1％的具有青霉素、链霉素和fungisol的溶液的容器中(在所述溶液中按揉所述罗非鱼皮5分钟)，并在转速为15转/分钟且温度为37℃的水浴(离心机)中另外保持3小时，并且在最后，将所述罗非鱼皮包装于无菌的双层塑料包封中并于4℃下储存以供日后使用。
4.根据权利要求1至3任一项所述的方法，其特征在于，若细菌计数高于10/3，应进行补充灭菌步骤。
5.根据权利要求4所述的方法，其特征在于，其是补充辐射灭菌步骤，伽马-钴60，其中负荷剂量范围为15至50千戈瑞，取决于微生物计数的生物负载水平。
6.根据前述权利要求所述的罗非鱼皮的用途，其特征在于，所述罗非鱼皮作为诸如烧伤和急性或慢性伤口的皮肤损伤的封闭敷料施用。
CN201680051079.8A 2015-09-03 2016-09-02 用于改进罗非鱼皮的方法及其用于覆盖皮肤损伤的用途 Pending CN108778351A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BRBR102015021435-9 2015-09-03
BR102015021435A BR102015021435B8 (pt) 2015-09-03 2015-09-03 Processo de beneficiamento da pele da tilápia e seu uso na cobertura de lesões cutâneas
PCT/BR2016/000084 WO2017035615A1 (pt) 2015-09-03 2016-09-02 Processo de beneficiamento'da pele da tilápia e seu uso na cobertura de lesões cutâneas

Publications (1)

Publication Number Publication Date
CN108778351A true CN108778351A (zh) 2018-11-09

Family

ID=58186353

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680051079.8A Pending CN108778351A (zh) 2015-09-03 2016-09-02 用于改进罗非鱼皮的方法及其用于覆盖皮肤损伤的用途

Country Status (4)

Country Link
US (1) US11135337B2 (zh)
CN (1) CN108778351A (zh)
BR (1) BR102015021435B8 (zh)
WO (1) WO2017035615A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113164643A (zh) * 2018-11-14 2021-07-23 埃德马尔·马西埃尔·利马·朱尼尔 获得冷冻干燥的动物皮肤的方法，冷冻干燥的动物皮肤，其用途和试剂盒

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107509987B (zh) * 2017-09-20 2020-07-07 中国水产科学研究院淡水渔业研究中心 一种具有生物活性无鳞囊罗非鱼皮的制作方法
US11612675B1 (en) 2018-06-28 2023-03-28 The Regents Of The University Of California Fish skin biologic bandage
BR102018073515A2 (pt) * 2018-11-14 2020-05-26 Edmar Maciel Lima Junior processo de obtenção de matriz extracelular de pele de tilápia (oreochromis niloticus) e uso da matriz extracelular de tilápia
CN114642624A (zh) * 2020-12-21 2022-06-21 颜荣宏 精炼胺基酸生物载体面膜及精炼胺基酸用于保养品的用途

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1068703A (zh) * 1991-07-25 1993-02-10 王权海 烧伤创面用鱼皮制备方法
CN1096458A (zh) * 1993-11-25 1994-12-21 徐国士 治疗烧伤(刀伤)创面的鱼皮(湿或干)异种皮及制造工艺
CN1159482A (zh) * 1996-03-08 1997-09-17 徐国士 鱼皮无毒鞣制工艺
CN102781485A (zh) * 2009-10-07 2012-11-14 克雷西斯公司 用于创伤护理和/或其它组织愈合应用的支架材料
EP2644692A1 (en) * 2010-11-26 2013-10-02 Tokyo Institute of Technology High-strength collagen fiber membrane and method for producing same
WO2013144727A2 (en) * 2012-03-30 2013-10-03 Kerecis Ehf A scaffold material graft for wound care and/or other tissue healing applications

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2731833B2 (ja) * 1995-06-26 1998-03-25 大同ほくさん株式会社 海洋生物を原料とした代用皮膚
EP2644620B1 (en) 2010-11-26 2017-06-28 Tokyo Institute of Technology Non-fibrogenesis collagen material and a manufacturing method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1068703A (zh) * 1991-07-25 1993-02-10 王权海 烧伤创面用鱼皮制备方法
CN1096458A (zh) * 1993-11-25 1994-12-21 徐国士 治疗烧伤(刀伤)创面的鱼皮(湿或干)异种皮及制造工艺
CN1159482A (zh) * 1996-03-08 1997-09-17 徐国士 鱼皮无毒鞣制工艺
CN102781485A (zh) * 2009-10-07 2012-11-14 克雷西斯公司 用于创伤护理和/或其它组织愈合应用的支架材料
EP2644692A1 (en) * 2010-11-26 2013-10-02 Tokyo Institute of Technology High-strength collagen fiber membrane and method for producing same
WO2013144727A2 (en) * 2012-03-30 2013-10-03 Kerecis Ehf A scaffold material graft for wound care and/or other tissue healing applications

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
潘求真等: "《细胞工程》", 31 July 2009, 哈尔滨工程大学出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113164643A (zh) * 2018-11-14 2021-07-23 埃德马尔·马西埃尔·利马·朱尼尔 获得冷冻干燥的动物皮肤的方法，冷冻干燥的动物皮肤，其用途和试剂盒

Also Published As

Publication number Publication date
US11135337B2 (en) 2021-10-05
BR102015021435B1 (pt) 2023-10-10
US20180272026A1 (en) 2018-09-27
WO2017035615A1 (pt) 2017-03-09
BR102015021435B8 (pt) 2023-11-07
BR102015021435A2 (pt) 2017-03-07

Similar Documents

Publication Publication Date Title
CN108778351A (zh) 用于改进罗非鱼皮的方法及其用于覆盖皮肤损伤的用途
Phillips et al. The effect of negative pressure wound therapy with periodic instillation using antimicrobial solutions on Pseudomonas aeruginosa biofilm on porcine skin explants
Wollina et al. Medical leech therapy (Hirudotherapy).
RU2609201C1 (ru) Способ получения остеопластического материала
CN103394116A (zh) 一种可促进伤口愈合的水凝胶敷料及其制备方法
CN101874903B (zh) 一种胶原蛋白人工皮肤的制备方法
CN104474573A (zh) 一种生物敷膜及制备方法
Tao et al. The role of whirlpool in wound care
KR101139434B1 (ko) 돼지피부를 이용한 드레싱재의 제조방법
CN113164643A (zh) 获得冷冻干燥的动物皮肤的方法，冷冻干燥的动物皮肤，其用途和试剂盒
TWI610674B (zh) 一種用於促進傷口癒合或預防、減少傷口疤痕生成的醫藥組合物
CN101548914B (zh) 无菌异种医用生物膜及其制备方法
Young Management of slough in diabetic foot wounds
Kabala-Dzik et al. Influence of honey-balm on the rate of scare formation during experimental burn wound healing in pigs
CN114303454B (zh) 生物制品dbt的病毒灭活方法
CN204050019U (zh) 止血、抗菌敷料贴
Oberreiter et al. The Efficacy of Hyaluronic Acid in Combination with an Antiseptic—Experience from Acute Military-Based Wound Care
Lee et al. Evaluating Effectiveness of Medical Grade Honey-Containing Alginate Dressing in Patients with Chronic Lower Extremity Wounds
Leaper et al. Debridement methods of non-viable tissue in wounds
Cavanaugh Postoperative Complications
Makhmudov et al. The features of autodermoplasty in traumatic wounds of the skin and soft tissues
Rusdy et al. The effectivity of ozone water application on tampon in post posterior tooth extraction in Department of Oral Surgery and Maxillofacial Faculty of Dentistry, University of North Sumatera (USU)
Bahri et al. Integrating Zinc Cream-Chitosan and Cadexomer Iodine for Treating Infections in Diabetic Foot Ulcers: A Case Report
Pal et al. Study of graft uptake in honey dressed wound: a 2 year experience
BR102019023961B1 (pt) Processo de obtenção de curativo biológico oclusivo, curativo biológico oclusivo, uso do mesmo e kit

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20210827

Address after: Ba Xisaialazhou

Applicant after: COMPANHIA ENERGETICA DO CEARA-COELCE

Applicant after: Edmar Maciel Lima Junior

Applicant after: Marcelo Jose Borges de Miranda

Applicant after: Saiala Federal University

Address before: Ba Xisaialazhou

Applicant before: COMPANHIA ENERGETICA DO CEARA-COELCE

Applicant before: INSTITUTO DE APOIO AO QUEIMADO-IAQ

Applicant before: Edmar Maciel Lima Junior

Applicant before: Manuel Odoric de Morais Filho

Applicant before: Marcelo Jose Borges de Miranda

Applicant before: Nelson Sarto Piccolo

TA01 Transfer of patent application right
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20181109

WD01 Invention patent application deemed withdrawn after publication