CN108732223B

CN108732223B - a biosensing device

Info

Publication number: CN108732223B
Application number: CN201810522756.0A
Authority: CN
Inventors: 刘明源
Original assignee: Shandong Boyuan Data Service Co Ltd
Current assignee: Ningxia Lanyin Biotechnology Co ltd
Priority date: 2018-05-28
Filing date: 2018-05-28
Publication date: 2020-05-01
Anticipated expiration: 2038-05-28
Also published as: CN108732223A

Abstract

The invention discloses a biological sensing device, which relates to the technical field of electrochemical devices. The invention comprises a device casing. A surface of the device casing is fixedly connected with a fixed detection needle, and a peripheral side surface of the fixed detection needle is rotatably connected with a plurality of connecting pieces. One end of the connecting piece is A movable probe needle is connected in rotation, a handle is fixedly connected on one surface of the device shell, a bottom cover is threadedly connected on the inner surface of the handle, an inner tank is movably connected with the handle, a flash memory chip is slidably connected on a surface of the inner tank, and a surface of the inner tank is electrically connected to the device shell. The present invention can adjust the quantity and shape according to the shape of the plant through the cooperation of the connecting piece and the movable probe needle, so that the sensing device can fit perfectly with the plant, and solves the problem of thin leaves, less tissue fluid, and lignification of stems. It leads to the problem of difficulty in detection, improves the efficiency of device detection, and makes the sensing device have the advantages of modular dynamic adjustment.

Description

Biological sensing device

Technical Field

The invention relates to the technical field of electrochemical devices, in particular to a biosensing device.

Background

Glucose is a monosaccharide, and is hexose containing aldehyde groups. It is widely present in plants, is the main component of some saccharides, and is the main energy substance in plants. Glucose is a product of photosynthesis and a substrate of respiration, provides energy for the growth and development of plants, and can enhance the stress resistance of the plants. Glucose metabolism is the center of whole plant metabolism and it is the communication of the metabolism of proteins, lipids and secondary products.

Currently, in plant physiological research, a colorimetric method, a DNS reagent reducing sugar measuring method, a liquid chromatography method and the like are mostly adopted for glucose detection. The method needs to sample the plant material in vitro, calculates the content in the plant body by detecting the concentration of glucose in the leaching solution, has high requirement on the extraction purity of the sample, has complex sample processing and long time consumption, obtains the concentration at a certain moment in the plant body, and cannot perform dynamic analysis and long-term tracking monitoring. In order to realize the research on plant growth and development and environmental adaptability, in-situ dynamic detection of living bodies is required, glucose living body detection products in the market at present mainly aim at animals or human bodies, and the detection products cannot be directly applied to the plant bodies due to the characteristics of thin leaves, less tissue fluid, lignification of stems and the like of the plant bodies, so that the development of a biosensing device for glucose detection of plant tissues is urgently needed.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides the biosensing device, the connecting pieces and the movable probe needles are adjusted in quantity and shape according to the shape of the detected plant, so that the sensing device can be perfectly attached to the plant, the detection efficiency of the device is improved, and the problems of thin plant leaves, less tissue fluid and difficult detection caused by lignification of stems are solved; the glucose concentration information that will survey the syringe needle detection that can be autonomic through the flash memory chip is stored and memory chip can store a large amount of experimental data and compare, and the problem that experimental data record process is complicated has been solved in the classification and the arrangement of the experimental data of being convenient for.

In order to achieve the purpose, the invention is realized by the following technical scheme: a biosensing device comprises a device shell, wherein a fixed detection needle is fixedly connected to one surface of the device shell, two connecting pieces are rotatably connected to the peripheral side surfaces of the fixed detection needle, and a movable detection needle is rotatably connected to one end of each connecting piece;

the device comprises a device shell and is characterized in that a handle is fixedly connected to one surface of the device shell, a bottom cover is in threaded connection with the inner surface of the handle, the handle is movably connected with an inner container, a flash memory chip is in sliding connection with one surface of the inner container, and one surface of the inner container is electrically connected with the device shell.

Furthermore, two connecting grooves are formed in the peripheral side face of the fixed probe needle.

Furthermore, the inside fixedly connected with three connecting element of device shell, connecting element one end and inner bag electric connection, the connecting element other end fixedly connected with gold working electrode, platinum counter electrode and silver reference electrode respectively, gold working electrode, platinum counter electrode and silver reference electrode one end all run through the device shell and extend to the inside of connecting groove, the inside fixedly connected with second of gold working electrode position in connecting groove connects the inner ring, the inside fixedly connected with first of one end fixedly connected with in connecting groove of platinum counter electrode position in connecting groove connects the inner ring.

Further, the connecting piece includes first pivot, first pivot internal surface is rotated with the connecting groove and is connected, a first pivot fixed surface is connected with the connecting rod, connecting rod one end fixedly connected with second pivot, second pivot internal surface is rotated with the activity probe and is connected.

Furthermore, a first connection outer ring and a second connection outer ring are fixedly connected to the inner surface of the first rotating shaft respectively, one surface of the first connection outer ring is fixedly connected with the platinum counter electrode, one surface of the second connection outer ring is fixedly connected with the gold working electrode, one end of the gold working electrode and one end of the gold working electrode, which are positioned inside the connecting rod, are fixedly connected with the second rotating shaft, and the internal connection structure of the second rotating shaft is consistent with that of the first rotating shaft.

Further, the activity probe includes the activity syringe needle, two activity recesses have been seted up to activity syringe needle week side, activity syringe needle one surface is rotated through activity recess and second pivot and is connected, activity syringe needle one end threaded connection has the stopper.

Further, the internal structure of the movable probe head except the silver reference electrode is consistent with that of the fixed probe needle.

Furthermore, two battery boxes are arranged on one surface of the inner container, a T-shaped sliding groove is arranged on one surface of the inner container, a metal contact is arranged inside the inner container, one surface of the inner container is in sliding connection with the flash memory chip through the T-shaped sliding groove, one surface of the inner container is in electric connection with the flash memory chip through the metal contact, and a threaded rubber sleeve is sleeved on the side face of the handle.

The invention has the following beneficial effects:

1. this biological sensing device, connecting piece and activity probe carry out quantity and shape adjustment according to detecting the plant shape for sensing device can with the perfect laminating of plant, solved that the plant leaf is thin, the interstitial fluid is few, the stem lignification leads to the problem of detecting the difficulty, improve the efficiency that the device detected, make sensing device have the advantage of modularization dynamic adjustment.

2. This biosensor device, the glucose concentration information that the probe needle detected that the flash memory chip can be autonomic is stored and memory chip can store a large amount of experimental data and compare, and the classification and the arrangement of the experimental data of being convenient for have the advantage of the experimental data collection of being convenient for.

3. According to the biological sensing device, the connecting groove and the internal structure of the movable groove enable the device to be additionally provided with a large number of movable detection needles without extra power supply, the connecting piece can be freely connected with the device, and the compatibility and the practicability of the device are improved.

Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic structural view of a biosensor device;

FIG. 2 is an exploded view of FIG. 1;

FIG. 3 is a schematic view of a fixed probe;

FIG. 4 is a cross-sectional view taken along line A-A of FIG. 3;

FIG. 5 is an enlarged view of a portion of FIG. 4 at A;

FIG. 6 is a schematic view of the structure inside the connection groove;

FIG. 7 is a schematic structural view of a connector;

FIG. 8 is a schematic view of a movable probe;

in the drawings, the components represented by the respective reference numerals are listed below:

in the figure: 1-device shell, 2-fixed probe, 3-connecting piece, 4-movable probe, 5-handle, 6-bottom cover, 7-inner container, 8-flash memory chip, 9-connecting element, 10-gold working electrode, 11-platinum counter electrode, 12-platinum counter electrode, 13-first connecting inner ring, 14-second connecting inner ring, 15-first connecting outer ring, 16-second connecting outer ring, 201-connecting groove, 301-first rotating shaft, 302-connecting rod, 303-second rotating shaft, 401-movable groove, 402-movable needle head, 403-limiting block.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be understood that the terms "open", "upper", "lower", "thickness", "top", "middle", "length", "inner", "periphery", "one surface", "one end surface", "one side surface", etc. indicate the orientation or positional relationship, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

Referring to fig. 1-2, the present invention provides a technical solution: the utility model provides a biosensing device, including device shell 1, 1 fixed surface of device shell is connected with

fixed probe needle

2, 2 all sides of fixed probe needle are rotated and are connected with two connecting

pieces

3, 3 one ends of connecting piece are rotated and are connected with movable probe needle 4, through installing more connecting pieces 3, make the device can connect more and the corresponding movable probe needle 4 of connecting piece 3 quantity, can effectual improvement detection efficiency to shaft-like or stem-like plant, movable probe needle 4 is through parallelly connected mode and 2 electric connection of fixed probe needle.

A handle 5 is fixedly connected to one surface of a device shell 1, a bottom cover 6 is connected to the inner surface of the handle 5 in a threaded mode, an inner container 7 is movably connected to the handle 5, a flash memory chip 8 is connected to one surface of the inner container 7 in a sliding mode, one surface of the inner container 7 is electrically connected with the device shell 1, a silicon wafer substrate is arranged inside the device shell 1 and used for detecting peak signals of current in a circuit, the obtained signals are transmitted to the flash memory chip 8, and the silicon wafer substrate is electrically connected with the inner container 7 respectively.

Wherein, two battery casees have been seted up on 7 surfaces of inner bag for the installation dry battery, T shape spout has been seted up on 7 surfaces of inner bag, inside is provided with the metal contact, 7 surfaces of inner bag pass through T shape spout and flash memory chip 8 sliding connection, 7 surfaces of inner bag pass through metal contact and flash memory chip 8 electric connection, flash memory chip 8 is MX25L1606EM2I-12G model flash memory chip, a data for recording plant glucose concentration, 5 side covers are equipped with the screw thread rubber sleeve around the handle, receive the hand sweat to influence when preventing to operate and skid.

As shown in fig. 3-6, two connecting grooves 201 are formed on the peripheral side of the fixed probe 2.

Wherein, the inner part of the device shell 1 is fixedly connected with three connecting elements 9, one end of each connecting element 9 is electrically connected with the inner container 7, the other end of each connecting element 9 is respectively and fixedly connected with a gold working electrode 10, a platinum counter electrode 11, a silver reference electrode 12 and a gold working electrode 10, the platinum counter electrode 11 is used for cyclic voltammetry detection, the detection voltage is in a range from-1.5V to 1.0V, the scanning speed is 60mV/s, the silver reference electrode 12 is used for potential reference, each connecting element 9 is independently connected with the corresponding electrode, one end of each of the gold working electrode 10, the platinum counter electrode 11 and the silver reference electrode 12 penetrates through the device shell 1 and extends to the inside of the connecting groove 201, one end of the gold working electrode 10, which is located inside the connecting groove 201, is fixedly connected with the second connecting inner ring 14, and one end of the platinum counter electrode 11, which is located inside the connecting groove 201, is fixedly connected with the first connecting inner ring 13.

The inner surface of the first rotating shaft 301 is fixedly connected with a first connection outer ring 15 and a second connection outer ring 16 respectively, the inner surface of the first connection outer ring 15 is rotatably connected with a first connection inner ring 13, the inner surface of the second connection outer ring 16 is rotatably connected with a second connection inner ring 14, one surface of the first connection outer ring 15 is fixedly connected with a platinum counter electrode 11, one surface of the second connection outer ring 16 is fixedly connected with a gold working electrode 10, one end of the gold working electrode 10 and one end of the gold working electrode 10, which are positioned in the connection rod 302, are fixedly connected with a second rotating shaft 303, and the internal connection structure of the second rotating shaft 303 is consistent with that of the first rotating shaft 301.

As shown in fig. 7, the connecting member 3 includes a first rotating shaft 301, an inner surface of the first rotating shaft 301 is rotatably connected to the connecting groove 201, a connecting rod 302 is fixedly connected to a surface of the first rotating shaft 301, one end of the connecting rod 302 is fixedly connected to a second rotating shaft 303, and an inner surface of the second rotating shaft 303 is rotatably connected to the movable probe 4.

As shown in fig. 8, the movable probe 4 includes a movable needle 402, two movable grooves 401 are formed on the peripheral side of the movable needle 402, one surface of the movable needle 402 is rotatably connected to the second rotating shaft 303 through the movable grooves 401, and a limit block 403 is connected to one end of the movable needle 402 through a thread.

The internal structure of the movable needle head 402 is consistent with that of the fixed probe needle 2 except the silver reference electrode 12, and the internal structure of the movable groove 401 is consistent with that of the connecting groove 201.

The working principle of the embodiment is as follows: the quantity of the connecting pieces 3 and the movable detection needles 4 is determined according to the plant shape to be measured, the two connecting pieces 3 are sleeved on the peripheral side face of the fixed detection needle 2, the limiting block 403 is twisted, the second rotating shaft 303 is sleeved in the movable groove 401, the limiting block 403 is screwed and fixed, the installation of the sensing device is completed, the needle heads of the fixed detection needle 2 and the movable detection needle 4 are inserted into a glucose oxidase solution to be soaked for 4 hours, after the soaking is completed, the handle 5 is held by the left hand, the sensing device is attached to the plant shape by rotating the connecting piece 3 by the right hand, each detection needle head can be fully contacted with the interior of the plant, the needle heads are inserted into the detection plant, the detection is performed through a cyclic voltammetry method, the detected signals are transmitted to the flash memory chip 8 through a silicon wafer substrate, and the detection of the glucose.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A biosensing device, comprising a device housing (1), characterized in that: a surface of the device housing (1) is fixedly connected with a fixed probe (2), and the peripheral side of the fixed probe (2) is provided with several A connecting groove (201), a plurality of connecting pieces (3) are rotatably connected to the peripheral side surface of the fixed detection pin (2), and a movable detection pin (4) is rotatably connected to one end of the connecting piece (3); the device housing ( 1) A handle (5) is fixedly connected to one surface, a bottom cover (6) is threadedly connected to the inner surface of the handle (5), an inner pot (7) is movably connected to the handle (5), and the inner pot (7) ) a surface of which is slidably connected with a flash memory chip (8), and a surface of the inner pot (7) is electrically connected to the device casing (1); One end of the element (9) is electrically connected to the inner pot (7), and the other end of the connecting element (9) is respectively fixedly connected with a gold working electrode (10), a platinum counter electrode (11) and a silver reference electrode (12), One ends of the gold working electrode (10), the platinum counter electrode (11) and the silver reference electrode (12) all penetrate through the device casing (1) and extend to the inside of the connection groove (201), and the gold working electrode (10) ) is located inside the connection groove (201) and is fixedly connected with a second connection inner ring (14), and the platinum counter electrode (11) is fixedly connected with a first connection inner ring (13) at one end inside the connection groove (201). ); the connecting piece (3) includes a first rotating shaft (301), the inner surface of the first rotating shaft (301) is rotatably connected with the connecting groove (201), and a surface of the first rotating shaft (301) is fixedly connected with a connecting rod (302), one end of the connecting rod (302) is fixedly connected with a second rotating shaft (303), and the inner surface of the second rotating shaft (303) is rotatably connected with the movable probe needle (4); the inner surface of the first rotating shaft (301) A first connection outer ring (15) and a second connection outer ring (16) are respectively fixedly connected, one surface of the first connection outer ring (15) is fixedly connected with the platinum counter electrode (11), and the second connection outer ring (11) is fixedly connected. One surface of the ring (16) is fixedly connected with the gold working electrode (10), and the gold working electrode (10) and the gold working electrode (10) are fixedly connected with the second rotating shaft (303) at one end located inside the connecting rod (302), The internal connection structure of the second rotating shaft (303) is consistent with the first rotating shaft (301).

2 . The biosensing device according to claim 1 , wherein the movable probe needle ( 4 ) comprises a movable needle head ( 402 ), and the movable needle head ( 402 ) is provided with a plurality of movable grooves on its peripheral side. 3 . (401), a surface of the movable needle (402) is rotatably connected to the second rotating shaft (303) through a movable groove (401), and one end of the movable needle (402) is threadedly connected to the limiting block (403).

3 . The biosensing device according to claim 2 , wherein the internal structure of the movable needle ( 402 ) is consistent with the fixed probe needle ( 2 ) except for the silver reference electrode ( 12 ). 4 .

4 . The biosensing device according to claim 3 , wherein a plurality of battery boxes are provided on a surface of the inner container ( 7 ), and a T-shaped chute is provided on a surface of the inner container ( 7 ). 5 . , a metal contact is provided inside, a surface of the inner pot (7) is slidably connected to the flash memory chip (8) through a T-shaped chute, and a surface of the inner pot (7) is connected to the flash memory chip (8) through a metal contact For electrical connection, a threaded rubber sleeve is sleeved on the peripheral side surface of the handle (5).