CN108651524A - The complex preparation and its preparation of a kind of jinggangmeisu and bacillus subtilis and application - Google Patents
The complex preparation and its preparation of a kind of jinggangmeisu and bacillus subtilis and application Download PDFInfo
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- CN108651524A CN108651524A CN201810429260.9A CN201810429260A CN108651524A CN 108651524 A CN108651524 A CN 108651524A CN 201810429260 A CN201810429260 A CN 201810429260A CN 108651524 A CN108651524 A CN 108651524A
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- jinggangmeisu
- bacillus subtilis
- complex preparation
- synergist
- bacillus
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- 235000014469 Bacillus subtilis Nutrition 0.000 title claims abstract description 117
- 238000002360 preparation method Methods 0.000 title claims abstract description 65
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- 235000009566 rice Nutrition 0.000 claims abstract description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 241000726221 Gemma Species 0.000 claims abstract description 28
- 239000008367 deionised water Substances 0.000 claims abstract description 15
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 15
- 239000002671 adjuvant Substances 0.000 claims abstract description 11
- 240000007594 Oryza sativa Species 0.000 claims abstract 2
- 239000003795 chemical substances by application Substances 0.000 claims description 27
- 239000004094 surface-active agent Substances 0.000 claims description 24
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- 239000000080 wetting agent Substances 0.000 claims description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 9
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- RYAGRZNBULDMBW-UHFFFAOYSA-L calcium;3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical group [Ca+2].COC1=CC=CC(CC(CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O RYAGRZNBULDMBW-UHFFFAOYSA-L 0.000 claims description 6
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- 238000013329 compounding Methods 0.000 description 7
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- 229940079593 drug Drugs 0.000 description 6
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- 239000007921 spray Substances 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
- 241000813090 Rhizoctonia solani Species 0.000 description 4
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000233732 Fusarium verticillioides Species 0.000 description 1
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- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
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- 241000607479 Yersinia pestis Species 0.000 description 1
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- VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to technical field of pesticide, and in particular to a kind of complex preparation of jinggangmeisu and bacillus subtilis includes the following component based on mass percentage：Jinggangmeisu 0.1~4%, bacillus subtilis, synergist 0.5~10%, adjuvant 3~30%, surplus are deionized water；The content of bacillus subtilis is 10~15,000,000,000 gemma/g in complex preparation.The present invention also provides a kind of preparation methods of the complex preparation of above-mentioned jinggangmeisu and bacillus subtilis, jinggangmeisu, bacillus subtilis, synergist and adjuvant are put into according to certain mass percent in deionized water, it is uniformly mixed to get complex preparation.The complex preparation of above-mentioned jinggangmeisu and bacillus subtilis can be used for preventing rice sheath blight disease or false smut.Complex preparation provided by the invention compounds the principal component as pesticide using the reagent of two kinds of different role mechanism of jinggangmeisu and bacillus subtilis, is conducive to the drug resistance for delaying germ to single medicament, to extend the Pesticide use period.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of complex preparation of jinggangmeisu and bacillus subtilis
And its it prepares and applies.
Background technology
Rice sheath blight disease and false smut are the Major Diseases in Rice Production.Rice sheath blight disease is one kind by miliary damping-off
Rice disease caused by bacterium Rhizctonia solani is one of three major disease of rice, in countries such as South America, Europe And Africas
There is generation with the main rice workspace in China, causes Rice Yield Loss Caused serious.Rice green smut is by ustilaginoidea virens
Rice Panicle fungal disease caused by Ustilaginoidea virens, is distributed widely in Asia, Africa, South America and Europe
Multiple countries of the rice main producing region such as continent, the generation in China aggravate year by year, and false smut not only influences the yield and product of rice
Matter, reduces rice commodity value, and ustilaginoidea virens is also toxic to people and animals.
Currently, the pharmacy variety for preventing banded sclerotial blight and false smut on rice is relatively single and medication is frequent, usage amount be in by
The trend that year rises.Single medicament, which is used for a long time, makes germ develop immunity to drugs, and not only cost accounting increases, and drug effect also can be significantly
It reduces, therefore market needs new medicament to prevent rice sheath blight disease and false smut.
Invention content
In order to overcome above-mentioned the shortcomings of the prior art, the object of the present invention is to provide a kind of jinggangmeisu and withered grass
The complex preparation of bacillus and its preparation and application can improve the drug effect of pesticide, extend the Pesticide use period, reduce
Cost.
To achieve the above object, the technical scheme is that a kind of compounding system of jinggangmeisu and bacillus subtilis
Agent includes the following component based on mass percentage：Jinggangmeisu 0.1~4%, bacillus subtilis, synergist 0.5~
10%, adjuvant 3~30%, surplus is deionized water；The content of bacillus subtilis is 10~150 in the complex preparation
Hundred million gemma/g.
Further, the adjuvant includes dispersant, antifreezing agent and wetting agent, and each component accounts for the complex preparation
The degree of gross mass is as follows：Dispersant 1.0~10.0%, antifreezing agent 1.0~10.0%, wetting agent 1.0~
10.0%.
Further, include following component based on mass percentage：Jinggangmeisu 1~4%, bacillus subtilis,
Synergist 0.5~7%, dispersant 2.0~8.0%, antifreezing agent 1.0~6.0%, wetting agent 1.0~7.0%, surplus be go from
Sub- water；The content of bacillus subtilis is 50~15,000,000,000 gemma/g in the complex preparation.
Further, include following component based on mass percentage：Jinggangmeisu 1~3%, bacillus subtilis,
Synergist 1~5%, dispersant 2.0~5.0%, antifreezing agent 3.0~6.0%, wetting agent 1.0~5.0%, surplus is deionization
Water；The content of bacillus subtilis is 80~14,000,000,000 gemma/g in the complex preparation.
Preferably, include following component based on mass percentage：Jinggangmeisu 2.5%, bacillus subtilis increase
Agent 5% is imitated, dispersant 3%, antifreezing agent 4%, wetting agent 3%, surplus is deionized water；Withered grass gemma in the complex preparation
The content of bacillus is 10,000,000,000 gemma/g.
Further, the synergist is in efficient surfactant, organosilicon synergist, N-Methyl pyrrolidone
Any one.
Further, the dispersant is calcium lignosulfonate or polycarboxylate, and the antifreezing agent is ethylene glycol or the third two
Alcohol, the wetting agent are any one in tween, fast T, nonylphenol polyoxyethylene ether.
The present invention also provides a kind of preparation method of the complex preparation of above-mentioned jinggangmeisu and bacillus subtilis, packets
Include following steps：
1) synergist and adjuvant are put into according to design flow in deionized water, 1h is sheared in high-speed shearing machine, obtained
To mixed liquor；
2) it in the mixed liquor for preparing jinggangmeisu, bacillus subtilis input step 1), is uniformly mixed to get compounding
Preparation.
The complex preparation of above-mentioned jinggangmeisu and bacillus subtilis can be used for preventing rice sheath blight disease or false smut.
The design principle of the present invention：Jinggangmeisu is a kind of low-toxin farm chemicals, it is a kind of antibiotic that actinomyces generate,
It, can be thin by thalline quickly after rice sheath blight disease and false smut germ mycelia touch jinggangmeisu with very strong absorbability
Born of the same parents absorb and are conducted in thalline, somatic cells normal growth and development are interfered and inhibit, to play the effect sterilized well
Fruit；Bacillus subtilis is biological agent, and strain is isolated from soil or axis, is generated during thalli growth withered
Lavendustin, polymyxins, nystatin, gramicidins isoreactivity substance, these active materials are to pathogenic bacteria or autogenous infection
Conditioned pathogen have apparent inhibiting effect；Jinggangmeisu and bacillus subtilis are two kinds of heterogeneities and different role
The two is compounded preferable solution germ resistance problems, delays the generation of drug resistance, and well ridge is mould by the fungicide of mechanism
Element and two kinds of ingredients of bacillus subtilis do not have toxic action to natural enemy, can be effectively protected natural enemy, to rice sheath blight disease and
False smut synergistic effect is notable；Compound synergic agent simultaneously, is effectively increased jinggangmeisu and bacillus subtilis in plant surface
Permeability and the surface tension that can reduce aqua dilution reduce contact angle between crop after medicine liquid spray, make liquid
It is easier to be attached to crop leaf surface, will not just be slid when being stained with blade, improve pesticide utilization rate, increase jinggangmeisu
With bacillus subtilis in the conduction in crop；Composite reagent is synergistic apparent, reduces applications of pesticide amount, reduces agriculture
Industry cost.
Compared with prior art, the invention has the advantages that：
(1) complex preparation provided by the invention is using two kinds of different role mechanism of jinggangmeisu and bacillus subtilis
Reagent compounds the principal component as pesticide, is conducive to the drug resistance for delaying germ to single medicament, to extend Pesticide use week
Phase；
(2) synergist is added in complex preparation provided by the invention, improves Pesticide Infiltration, enhanced bactericidal effect, have
Conducive to the usage amount of pesticide is reduced, agricultural cost is reduced；
(3) Recompounded pesticide product efficacy stability provided by the invention, low toxicity is environmentally safe, to people and animals' no pathogenicity,
To diseases and pests of agronomic crop natural enemies security.
Description of the drawings
Field arrangement figure experimental field when the prevention rice sheath blight disease that Fig. 1 provides for the embodiment of the present invention nine；
Field arrangement figure experimental field when the prevention rice green smut that Fig. 2 provides for the embodiment of the present invention nine.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
All other embodiment that logical technical staff is obtained without making creative work, belongs to protection of the present invention
Range.
The embodiment of the present invention provides a kind of complex preparation of jinggangmeisu and bacillus subtilis, including presses quality percentage
The following component of content meter：Jinggangmeisu 0.1~4%, bacillus subtilis, synergist 0.5~10%, adjuvant 3~
30%, surplus is deionized water；The content of bacillus subtilis is 10~15,000,000,000 gemma/g in the complex preparation.
Further, the adjuvant includes dispersant, antifreezing agent and wetting agent, and each component accounts for the complex preparation
The degree of gross mass is as follows：Dispersant 1.0~10.0%, antifreezing agent 1.0~10.0%, wetting agent 1.0~
10.0%.
Further, include following component based on mass percentage：Jinggangmeisu 1~4%, bacillus subtilis,
Synergist 0.5~7%, dispersant 2.0~8.0%, antifreezing agent 1.0~6.0%, wetting agent 1.0~7.0%, surplus be go from
Sub- water；The content of bacillus subtilis is 50~15,000,000,000 gemma/g in the complex preparation.
Further, include following component based on mass percentage：Jinggangmeisu 1~3%, bacillus subtilis,
Synergist 1~5%, dispersant 2.0~5.0%, antifreezing agent 3.0~6.0%, wetting agent 1.0~5.0%, surplus is deionization
Water；The content of bacillus subtilis is 80~14,000,000,000 gemma/g in the complex preparation.
The present invention also provides a kind of preparation method of the complex preparation of above-mentioned jinggangmeisu and bacillus subtilis, packets
Include following steps：
1) synergist and adjuvant are put into according to design flow in deionized water, 1h is sheared in high-speed shearing machine, cut
Cutting speed degree is 2100rpm, obtains mixed liquor；
2) it in the mixed liquor for preparing jinggangmeisu, bacillus subtilis input step 1), is uniformly mixed to get compounding
Preparation.
The complex preparation of above-mentioned jinggangmeisu and bacillus subtilis can be used for preventing rice sheath blight disease or false smut.
Embodiment one
Complex preparation provided in this embodiment includes the following component based on mass percentage：Jinggangmeisu 2.5%,
Bacillus subtilis, efficient surfactant 5%, calcium lignosulfonate 3%, ethylene glycol 4%, tween 3%, surplus be go from
Sub- water；The content of bacillus subtilis is 10,000,000,000 gemma/g in the complex preparation.
Embodiment two
Complex preparation provided in this embodiment includes the following component based on mass percentage：Jinggangmeisu 0.1%,
Bacillus subtilis, organosilicon synergist 0.5%, polycarboxylate 2%, ethylene glycol 1%, tween 7%, surplus is deionized water；
The content of bacillus subtilis is 1,000,000,000 gemma/g in the complex preparation.
Embodiment three
Complex preparation provided in this embodiment includes the following component based on mass percentage：Jinggangmeisu 4%, it is withered
Careless bacillus, N-Methyl pyrrolidone 7%, calcium lignosulfonate 1%, propylene glycol 10%, tween 10%, surplus be go from
Sub- water；The content of bacillus subtilis is 15,000,000,000 gemma/g in the complex preparation.
Example IV
Complex preparation provided in this embodiment includes the following component based on mass percentage：Jinggangmeisu 1%, it is withered
Careless bacillus, N-Methyl pyrrolidone 10%, polycarboxylate 8%, ethylene glycol 7%, tween 1%, surplus is deionized water；
The content of bacillus subtilis is 5,000,000,000 gemma/g in the complex preparation.
Embodiment five
Complex preparation provided in this embodiment includes the following component based on mass percentage：Jinggangmeisu 3%, withered grass
Bacillus, efficient surfactant 5%, calcium lignosulfonate 2%, ethylene glycol 3%, fast T5%, surplus is deionized water；
The content of bacillus subtilis is 14,000,000,000 gemma/g in the complex preparation.
Embodiment six
Complex preparation provided in this embodiment includes the following component based on mass percentage：Jinggangmeisu 2%, it is withered
Careless bacillus, organosilicon synergist 1%, calcium lignosulfonate 5%, propylene glycol 5%, nonylphenol polyoxyethylene ether 4% are remaining
Amount is deionized water；8,000,000,000 gemma of content/g of bacillus subtilis in the complex preparation.
Embodiment seven
The present embodiment investigated different ratio jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises to rice banded sclerotial blight
The virulence testing experiment of disease and rice green smut, detailed process are as follows：
1. instrument and equipment
Conical flask, graduated cylinder, culture medium, glass bar, electronic balance (a ten thousandth), biological medium, culture dish, liquid relief
Device, inoculator, card punch, slide calliper rule etc..
2. experimental condition and material
2.1 biological reagent
Rhizoctonia solani Kuhn (Rhizoctonia solaniK (u) hn.) preserves bacterium by Chinese Academy of Sciences's Wuhan virus
Kind, 25 DEG C on PDA, constant temperature incubation uses after 72 hours.
Ustilaginoidea virens (Ustilaginoidea virens (Cooke) Takahashi) are Chinese Academy of Sciences Wuhan
The preserved strain of virus, 28 DEG C on PDA, constant temperature incubation uses after 10 days.
2.2 test medicine
60% jinggangmycin A active compound, 200,000,000,000/g bacillus subtilis original powders.
3. test procedure
3.1 medicaments are prepared：
Jinggangmeisu and bacillus subtilis are configured to the mother liquor of different ratio in table 1 with sterile water dissolution, according to pre-
It does experiment as a result, this 7 processing of A, B, C, D, E, F, G are diluted to 5 mass concentration gradients with sterile water again：2500、
5000,10000,20000,40000 μ g/mL measure compound drug virulence according to mycelial growth rate method.Wherein, withered grass gemma
The use of bacillus subtilis bacterium solution is made in bacillus.
1 jinggangmeisu of table is handled with bacillus subtilis medicament different ratio
3.2 chemicals treatment
Compound drug virulence is measured according to mycelial growth rate method, heating fusing quantitative culture medium 49mL, it is cooled to 50~
1mL reagent agents are added at 55 DEG C, it (is 1 weight of such processing per ware to be poured into after fully shaking up in 4 sterilizes culture dishes
It is multiple).After to be solidified, mark each ware medicament title and concentration (former diluted concentration gradient dilutes 50 times again, i.e., 50,100,200,
400、800μg/mL).The culture medium of control group adds 1mL sterile waters.
With sterile working, rice sheath blight disease with transfer needle take one piece of bacteria cake (bacteria cake diameter 0.5cm) accomplished fluently in advance in
Mixed medicine culture medium flat plate center, is subsequently placed in training in 25 DEG C of biochemical cultivation cases, and right-angled intersection rule measures each processing after 72h
Colony diameter；Rice green smut takes one piece of bacteria cake (bacteria cake diameter 0.5cm) accomplished fluently in advance in mixed medicine culture medium with transfer needle
Tablet center is subsequently placed in training in 28 DEG C of biochemical cultivation cases, and right-angled intersection rule measures each processing colony diameter after 14 days.
3.3 investigation and calculating：
Investigation result calculates mycelial growth inhibition rate of each concentration for the treatment of to fusarium moniliforme by formula (1), (2)：
Bacterium colony increases diameter=colony diameter-bacteria cake diameter ... ... ... ... ... ... ... ... (1)
Mycelial growth inhibition rate (%)=blank control bacterium colony increases diameter-processing bacterium colony and increases diameter) ÷ blank controls
Bacterium colony increases diameter × 100 ... ... ... ... ... ... ... ... ... (2)
4.2 statistical analysis
Make regression analysis according to each drug concentration logarithm and corresponding mycelial growth inhibition rate niqueMin, calculates each medicine
The ED of agent50, ED95Equivalent and its 95% confidence limit.
Co-toxicity coefficient assay method is carried out by Sun Yunpei (1960) method, and the specific method is as follows：
Using A as standard agent, then the theoretical toxicity index of A is 100.
The theoretical toxicity index (TI) of B=(A medicaments ED50÷ B medicaments ED50)×100
(A+B) actual toxicity index (ATI) of mixture=(A medicaments ED50÷ mixtures ED50)×100
(A+B) TI × A medicaments of theoretical toxicity index (TTI)=A medicaments of mixture account for TI × B of mixture %+B medicaments
Medicament accounts for mixture %
(A+B) co-toxicity coefficient of mixture=(ATI ÷ TTI) × 100
Co-toxicity coefficient indicates synergistic effect when being more than 120, summation action is indicated between 80~120, light rain is when 80
For antagonism.
2 results and analysis
2.1 The Toxicity Determination result
The co-toxicity of jinggangmeisu and bacillus subtilis different ratio is measured by mycelial growth rate method, as a result
As shown in table 2, table 3.
As can be seen from Table 2,2.5% jinggangmeisu of different ratio and 10,000,000,000/g bacillus subtilises compounding are to rice
Banded sclerotial blight germ indoor virulence is different, and each proportioning has certain synergistic effect, with 2.5% jinggangmeisu and 10,000,000,000/g
Bacillus subtilis proportioning is 1:Co-toxicity coefficient is maximum when 1, and CTC values are 186.22, and synergy is the most notable.ED50For 126.74 μ
G/mL, ED95It is 0.9923 for 1144.92 μ g/mL, R values.
The joint toxicity measuring result of 2 jinggangmeisu bacillus subtilis mixture rice sheath blight disease of table
The joint toxicity measuring result of 3 jinggangmeisu bacillus subtilis mixture rice green smut of table
As can be seen from Table 3,2.5% jinggangmeisu of different ratio and 10,000,000,000/g bacillus subtilises compounding are to rice
False smut germ indoor virulence is different, and 2.5% jinggangmeisu and 10,000,000,000/g bacillus subtilises are with 1:1、 2:1 and 3:1 three
Proportioning has certain synergistic effect, especially with 2.5% jinggangmeisu and 10,000,000,000/g bacillus subtilises proportioning for 1:Poison is altogether when 1
Number is maximum, and CTC values are 141.35, and synergy is the most notable.ED50For 150.47 μ g/mL, ED95It is for 6191.97 μ g/mL, R
0.9966。
Embodiment eight
The present embodiment explores 2.5% jinggangmeisu of synergist pair and 10,000,000,000 spores/g bacillus subtilises are compound aqueous
Synergistic effect, in addition to reagent agent is different, test apparatus material, application method, investigation method are identical as 1 step of embodiment.
Reagent agent is that 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous, and efficient surfactant is organic
Silicon synergist, N-Methyl pyrrolidone.
Liquid configuration method：By 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises it is compound aqueous with it is different
Synergist is configured to the mother liquor of different ratio in table 4 with sterile water dissolution, according to pre-made test result, then with sterile water by A,
B, 5 processing of C, D, E are diluted to 5 mass concentration gradients：50,100,200,400,800 μ g/mL grow speed according to mycelia
Rate method measures compound drug co-toxicity.
4 reagent agent experimental design of table
| Processing number | Medicament |
| A | 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous |
| B | 2.5% jinggangmeisu and compound aqueous+5% efficient surfactant of 10,000,000,000 spores/g bacillus subtilises |
| C | 2.5% jinggangmeisu and compound aqueous+7% organosilicon synergist of 10,000,000,000 spores/g bacillus subtilises |
| D | 2.5% jinggangmeisu and the compound aqueous+1.5%N- methyl pyrrolidones of 10,000,000,000 spores/g bacillus subtilises |
| E | Clear water (blank control) |
Chemicals treatment and statistical analysis technique are same as Example 1.By mycelial growth rate method measure jinggangmeisu with
The co-toxicity of bacillus subtilis different ratio, as a result as shown in table 5, table 6.
The joint toxicity measuring result of table 5 jinggangmeisu bacillus subtilis mixture and synergist rice sheath blight disease
The joint toxicity measuring result of table 6 jinggangmeisu bacillus subtilis mixture and synergist rice green smut
2.5% jinggangmeisu is different from the compound aqueous addition of 10,000,000,000 spores/g bacillus subtilises as can be seen from Table 5
Synergist is different to the growth inhibitory effect of Rhizoctonia solani Kuhn, to add 5% efficient surfactant compounding to rice banded sclerotial blight
Sick virulence index highest is 202.86, there is synergistic effect, ED50Value is 54.85 μ g/ml, relative coefficient 0.9866.
2.5% jinggangmeisu is different from the compound aqueous addition of 10,000,000,000 spores/g bacillus subtilises as can be seen from Table 6
Synergist is different to the growth inhibitory effect of rice green smut germ, to add 5% efficient surfactant compounding to rice rice
Bent disease virulence index highest, is 216.68, there is synergistic effect, ED50Value is 40.57 μ g/ml, and relative coefficient is
0.9967。
It is most suitable to efficient surfactant below to carry out screening test using concentration.
2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises is compound aqueous with different synergist sterile waters
Dissolving is configured to the mother liquor of different ratio in table 7, according to pre-made test result, then with sterile water by A, B, C, D, E, F, G 7
Processing is diluted to 5 mass concentration gradients：50,100,200,400,800 μ g/mL are measured according to mycelial growth rate method and are compounded
Drug combination virulence.
72.5% 10,000,000,000 spores of jinggangmeisu of table/g bacillus subtilises aqua is multiple with various concentration efficient surfactant
Match
| Processing number | Medicament |
| A | 2.5% 10,000,000,000 spores of jinggangmeisu/g bacillus subtilises are compound aqueous |
| B | Compound aqueous+1.0% efficient surfactant of 2.5% 10,000,000,000 spores of jinggangmeisu/g bacillus subtilises |
| C | Compound aqueous+2.5% efficient surfactant of 2.5% 10,000,000,000 spores of jinggangmeisu/g bacillus subtilises |
| D | Compound aqueous+5.0% efficient surfactant of 2.5% 10,000,000,000 spores of jinggangmeisu/g bacillus subtilises |
| E | Compound aqueous+7.5% efficient surfactant of 2.5% 10,000,000,000 spores of jinggangmeisu/g bacillus subtilises |
| F | Compound aqueous+10.0% efficient surfactant of 2.5% 10,000,000,000 spores of jinggangmeisu/g bacillus subtilises |
| G | Clear water (blank control) |
Chemicals treatment and statistical are same as above, this test result is as shown in table 8 and table 9.
8 various concentration efficient surfactant of table is to jinggangmeisu bacillus subtilis mixture aqua to rice sheath blight disease
Joint toxicity measuring result
9 various concentration efficient surfactant of table is to jinggangmeisu bacillus subtilis mixture aqua to rice green smut
Joint toxicity measuring result
As can be seen from Table 8,2.5% jinggangmeisu is different from the compound aqueous addition of 10,000,000,000 spores/g bacillus subtilises
Concentration efficient surfactant is different to the growth inhibitory effect of Rhizoctonia solani Kuhn, but additive amount reaches 5.0% later subsequently
Synergistic effect is not obviously improved, and illustrates that adding 5.0% efficient surfactant in the formulation can reach synergy purpose.It is excellent
Select efficient surfactant a concentration of 5.0%.
As can be seen from Table 9,2.5% jinggangmeisu is different from the compound aqueous addition of 10,000,000,000 spores/g bacillus subtilises
Concentration efficient surfactant is different to the growth inhibitory effect of Ustilaginoidea virens, but additive amount reaches 5.0% later subsequently
Synergistic effect is not obviously improved, and illustrates that adding 5.0% efficient surfactant in the formulation can reach synergy purpose.It is excellent
Select efficient surfactant a concentration of 5.0%.
In the present embodiment shown in data, 5.0% efficient surfactant can significantly improve jinggangmeisu and withered grass gemma
The compound aqueous drug effect of bacillus reduces insecticide dosage 20%-60% in same preventive effect, effectively reduces effective dosage after class, gram
Take and delay the drug resistance of harmful organism.
Embodiment nine
The present embodiment explores 2.5% jinggangmeisu and the compound aqueous prevention rice of 10,000,000,000 spores/g bacillus subtilises
The best field dosage or concentration, detailed process of banded sclerotial blight and rice green smut are as follows：
(1) rice sheath blight disease is prevented
This experiment controlling object is the rice sheath blight disease caused by Rhizoctonia solani K (u) hn.；Experiment is made
Object be it is early make transplant field rice, during experiment growth period duration of rice be in late tillering state to full heading time, kind is " Yu Xi glues ".Examination
It tests in the progress of the rice test field of agricultural college of Guangxi University teaching base, experimental field and its surrounding rice cultivation for years.
Test medicine is that 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous, and comparison medicament is
3% jinggangmeisu aqua and 100,000,000,000 gemma/g bacillus subtilis wettable powders, to spray clear water as blank control.
10 reagent agent experimental design of table
| Processing number | Medicament | Active ingredient usage amount |
| 1 | 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous | 2250mL preparations/hectare |
| 2 | 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous | 3000mL preparations/hectare |
| 3 | 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous | 4500mL preparations/hectare |
| 4 | 3% jinggangmeisu aqua (comparison medicament) | 150g/ hectares |
| 5 | 100000000000 gemma/g bacillus subtilises wettable powder (comparison medicament) | 180g/ hectares |
| 6 | Blank control | — |
4 district's groups will be experimental field divided into, each district's groups are divided into 6 cells, totally 24 cells.Per plot area 20m2,
Often handle 4 repetitions.Each processing cell random alignment, field arrangement figure is as shown in Figure 1, wherein I, II, III, IV respectively represents 4
Secondary repetition；1, it is blank control (CK) that 2,3,4,5 medicaments respectively represented, which are shown in Table 10,6,.
This experiment uses spray-on process dispenser, each medicament is watered by experimental design dosage (referring to table 10) after stirring and evenly mixing,
Using knapsack manual compression sprayer respectively to plant dispenser, a medicine was applied every 10 days, is used continuously 3 times.Liquid dosage is
750 liters/hectare, blank control spray the clear water of equivalent.Experiment altogether carry out 4 Disease investigations, the 1st time investigation be dispenser before send out
Sick radix investigation, later 3 investigation are respectively the investigation after dispenser three times.According to rice leaf sheath and blade hazard symptoms degree
Classification uses 5 accidental samplings per cell as unit of strain, and every is investigated 50 plants, totally 250 plants, records diseased plant number respectively
With sick series.
The data of this pilot survey share disease index (hereinafter referred to as disease refers to) and control effect (hereinafter referred to as preventive effect) 2
Kind, computational methods are as follows：
Ck0With Pt0The disease of clear water check plot and chemicals treatment district refers to respectively before medicine；Ck1With Pt1Respectively clear water after medicine
The disease of check plot and chemicals treatment district refers to.
112.5% jinggangmeisu of table and 10,000,000,000 spores/compound aqueous preventive effect to rice sheath blight disease of g bacillus subtilises
Note：Different large and small letters respectively represent significance of difference level up to 0.01 and 0.05.
As shown in Table 11,2.5% jinggangmeisu of test medicine and 10,000,000,000 spores/g bacillus subtilises are compound aqueous right
Rice sheath blight disease has preferable effect of control.2.5% jinggangmeisu of test medicine and 10,000,000,000 spores/g bacillus subtilises are multiple
With aqua under the dosage of 3000 ml of formulation/hectare and 4500 ml of formulation/hectare, until 10 days after last medicine, preventive effect
Up to 72% or more, preventive effect and the 3% jinggangmeisu water of comparison medicament of 3000 ml of formulation of test medicine/hectare treatment dosage
The preventive effect of 150 grams/ha of agent active ingredient and 100,000,000,000 gemma/180 g of formulation of g bacillus subtilis wettable powders/hectare
Quite.
(2) rice green smut is prevented
Controlling object is the rice green smut caused by Ustilaginoidea virens.For study object be boot stage extremely
The transplant field rice of the stage of yellow ripeness, experimental cultivar are early rice hybrid paddy rice " special excellent 275 ".Experiment is in the Nanning City Xingning District towns San Tang six
It is carried out in the rice terrace on the village slopes Bao Gai.
Test medicine is that 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous, and comparison medicament is
3% jinggangmeisu aqua, 100,000,000,000 gemma/g bacillus subtilises wettable powder and 430g/ rise tebuconazole suspension concentrates (good power
Gram), to spray clear water as blank control.
12 reagent agent experimental design of table
| Processing number | Medicament | Active ingredient usage amount |
| 1 | 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous | 2250mL/ hectares |
| 2 | 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous | 3000mL/ hectares |
| 3 | 2.5% jinggangmeisu and 10,000,000,000 spores/g bacillus subtilises are compound aqueous | 4500mL/ hectares |
| 4 | 3% jinggangmeisu aqua (comparison medicament) | 150g/ hectares |
| 5 | 100000000000 gemma/g bacillus subtilises wettable powder (comparison medicament) | 180g/ hectares |
| 6 | 430g/ rises tebuconazole suspension concentrates (comparison medicament) | 150.2mL/ hectare |
| 7 | Blank control | — |
4 district's groups will be experimental field divided into, each district's groups are divided into 7 cells, totally 28 cells.Per plot area 20m2,
Often handle 4 repetitions.Each processing cell random alignment, field arrangement figure is as shown in Fig. 2, wherein I, II, III, IV respectively represents 4
Secondary repetition；1, it is blank control (CK) that 2,3,4,5,6 medicaments respectively represented, which are shown in Table 12,7,.
This experiment uses spray-on process dispenser, each medicament is watered by experimental design dosage (referring to table 12) after stirring and evenly mixing,
It is each in Rise's boot period, heading cut phase and Qi Sui pustulation periods respectively using knapsack manual compression sprayer respectively to plant dispenser
A medicine is applied, is used continuously 3 times.Liquid dosage is 750 liters/hectare, and blank control sprays the clear water of equivalent.This experiment is to rice
The Disease investigation of bent disease is carried out in the rice stage of yellow ripeness.5 accidental samplings are used per cell, take 20 clumps per sample point, totally 100
Clump, calculates separately sick fringe (grain) rate and fringe (grain) preventive effect of each processing.
2 kinds of shared sick fringe (grain) rate of the data parameter of this pilot survey and preventive effect, computational methods are as follows：
Ck and Pt is respectively sick fringe (grain) rate of clear water check plot and chemicals treatment district before medicine.
132.5% jinggangmeisu of table and 10,000,000,000 spores/compound aqueous preventive effect to rice green smut of g bacillus subtilises
Note：Different large and small letters respectively represent significance of difference level up to 0.01 and 0.05.
As shown in Table 13,2.5% jinggangmeisu of test medicine and 10,000,000,000 spores/g bacillus subtilises it is compound aqueous
Under the dosage of 3000 ml of formulation/hectare and 4500 ml of formulation/hectare, until 12 days after last medicine, to rice green smut
The preventive effect of sick fringe is up to 75% or more, to the preventive effect of rice green smut infected seed up to 81% or more.3000 ml of formulation/hectare
Preventive effect and 3% 150 grams/ha of jinggangmeisu aqua active ingredient of comparison medicament, 430g/ rise tebuconazole suspension concentrates effectively at
Divide 64.6mL/ hectares of preventive effect projects not reach the significance level of difference, but is significantly higher than 2250 ml of formulation/hectare and comparison medicament
The preventive effect of 100000000000 gemma/180 g of formulation of g bacillus subtilis wettable powders/hectare.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (9)
1. the complex preparation of a kind of jinggangmeisu and bacillus subtilis, which is characterized in that include based on mass percentage
Following component：Jinggangmeisu 0.1~4%, bacillus subtilis, synergist 0.5~10%, adjuvant 3~30%, surplus are to go
Ionized water；The content of bacillus subtilis is 10~15,000,000,000 gemma/g in the complex preparation.
2. the complex preparation of a kind of jinggangmeisu and bacillus subtilis as described in claim 1 any one, feature exist
In：The adjuvant includes dispersant, antifreezing agent and wetting agent, and each component accounts for the percentage of the complex preparation gross mass and contains
Amount is as follows：Dispersant 1.0~10.0%, antifreezing agent 1.0~10.0%, wetting agent 1.0~10.0%.
3. a kind of complex preparation of jinggangmeisu and bacillus subtilis as claimed in claim 2, which is characterized in that including pressing
The following component of mass percentage meter：Jinggangmeisu 1~4%, bacillus subtilis, synergist 0.5~7%, dispersant 2.0
~8.0%, antifreezing agent 1.0~6.0%, wetting agent 1.0~7.0%, surplus are deionized water；Withered grass bud in the complex preparation
The content of spore bacillus is 50~15,000,000,000 gemma/g.
4. a kind of complex preparation of jinggangmeisu and bacillus subtilis as claimed in claim 3, which is characterized in that including pressing
The following component of mass percentage meter：Jinggangmeisu 1~3%, bacillus subtilis, synergist 1~5%, dispersant 2.0~
5.0%, antifreezing agent 3.0~6.0%, wetting agent 1.0~5.0%, surplus are deionized water；Withered grass gemma in the complex preparation
The content of bacillus is 80~14,000,000,000 gemma/g.
5. a kind of complex preparation of jinggangmeisu and bacillus subtilis as claimed in claim 4, which is characterized in that including pressing
The following component of mass percentage meter：Jinggangmeisu 2.5%, bacillus subtilis, synergist 5% are dispersant 3%, antifreeze
Agent 4%, wetting agent 3%, surplus are deionized water；The content of bacillus subtilis is 10,000,000,000 gemma/g in the complex preparation.
6. the complex preparation of a kind of jinggangmeisu and bacillus subtilis as described in claim 1-5 any one, feature
It is：The synergist is any one in efficient surfactant, organosilicon synergist, N-Methyl pyrrolidone.
7. the complex preparation of a kind of jinggangmeisu and bacillus subtilis as described in claim 2-5 any one, feature
It is：The dispersant is calcium lignosulfonate or polycarboxylate, and the antifreezing agent is ethylene glycol or propylene glycol, the wetting agent
For any one in tween, fast T, nonylphenol polyoxyethylene ether.
8. the preparation method of the complex preparation of jinggangmeisu and bacillus subtilis as described in claim 1-7 any one,
It is characterized by comprising the following steps：
1) synergist and adjuvant are put into according to design flow in deionized water, 1h is sheared in high-speed shearing machine, mixed
Close liquid；
2) it in the mixed liquor for preparing jinggangmeisu, bacillus subtilis input step 1), is uniformly mixed to get complex preparation.
9. the complex preparation of jinggangmeisu and bacillus subtilis as described in claim 1-7 any one is for preventing rice
Banded sclerotial blight or false smut.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110106115A (en) * | 2019-05-13 | 2019-08-09 | 中国农业科学院烟草研究所 | A kind of bacillus subtilis synergist and its preparing the application in bacillus subtilis microbial agent |
| CN110402929A (en) * | 2019-08-21 | 2019-11-05 | 浙江省桐庐汇丰生物科技有限公司 | A kind of bactericidal composition and its application containing jinggangmeisu |
| CN112175886A (en) * | 2020-11-02 | 2021-01-05 | 浙江省桐庐汇丰生物科技有限公司 | Method for culturing bacillus subtilis by using validamycin plate-frame filter residues |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1468524A (en) * | 2002-09-30 | 2004-01-21 | 江苏省农业科学院 | A microbial compound pesticide for preventing and treating rice sheath blight and rice false smut |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1468524A (en) * | 2002-09-30 | 2004-01-21 | 江苏省农业科学院 | A microbial compound pesticide for preventing and treating rice sheath blight and rice false smut |
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| CN110402929A (en) * | 2019-08-21 | 2019-11-05 | 浙江省桐庐汇丰生物科技有限公司 | A kind of bactericidal composition and its application containing jinggangmeisu |
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| CN114181856A (en) * | 2021-11-30 | 2022-03-15 | 南京农业大学 | Microbial composition for efficiently inhibiting odor production by biomass fermentation from source and application thereof |
| CN114181856B (en) * | 2021-11-30 | 2023-12-15 | 南京农业大学 | Microbial combinations that effectively inhibit odor production from biomass fermentation at the source and their applications |
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