Disclosure of Invention
Aiming at the defects and actual demands of the prior art, the invention provides a detection device, in particular to a rapid and convenient detection device, which can judge the placement position of a detection consumable in a way of reading a transparent bar code, automatically judge a method for starting detection timing, is convenient to operate, and has more intellectualization in recording and analyzing detection results and health management of users.
To achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a detection device, and in particular relates to a fast and convenient detection device, where the detection device includes a smart phone 1 with a photographing function, a detector base 2, and a detection consumption 3;
The detector base 2 is positioned below the smart phone 1 and comprises a detection window 18 positioned at the top, a signal transmission module 20 positioned at the bottom and a supporting part 19 positioned at the side;
The detection consumable is positioned right above a signal transmission module 20 at the bottom of the detector base 2 and comprises a hydrophobic boundary 13, a hydrophobic and oleophobic boundary 10, a pretreatment area 11, a detection area 14, a micro-channel 12 and a water absorption area 16, wherein a bar code patch 15 capable of transmitting the change of the detection area is adhered to the water absorption area 16;
when detecting the sample, the camera of smart mobile phone 1 shoots the detection consumable 3 directly over signal transduction module 20 through detecting window 18, discerns the bar code, and the camera of smart mobile phone 1, detecting window 18, detection consumable 3 and signal transduction module 20 are located same vertical straight line, and the side can accomplish the detection.
In the invention, the inventor performs structural optimization and functional exploration on the biochemical immunity detection device due to the heaviness and tedious operation steps of the detection device in the long-term clinical detection practice process, combines the bar code with the detection consumable, and assembles the detection device with the detector base and the smart phone into a complete system, so as to provide the detection device capable of automatically and accurately determining the biochemical detection time, realize detection record and feedback, be more convenient for health management of users, ensure the reaction time required by the optimal detection effect, and reduce the interference of the artificial judgment detection time on the detection result.
Preferably, the material of the supporting portion 19 is black opaque plastic, and the opaque material prevents light from affecting the detection result.
Preferably, the detecting consumable 3 adopts a multi-layer structure compounding mode, and the following steps are adopted from top to bottom: an upper cover plate 4, a filtering membrane 9, a detection substrate 5 and a lower cover plate 6.
Compared with the conventional dry biochemical test paper, the detection consumable 3 realizes better separation effect of blood cells and serum in the biochemical index analysis process in the lateral chromatographic flow process in the horizontal direction.
Preferably, the signaling module 20 includes the components required to detect process signaling, preferably a light source, an electronic sensor, and a battery.
Preferably, the top and bottom of the detector base 2 are provided with manual adjustment modules, which are used for adjusting the detection window 18 and the signal transmission module 20 according to the different camera positions of the mobile phone 1, and the adjustment steps are as follows: firstly, the adjusting device at the top is used for adjusting the detection window 18, so that the camera of the mobile phone 1 can be completely exposed in the detection window, and the adjusting device at the top is fixed at the moment; the position of the signal transduction block 20 is then adjusted by means of the adjusting means at the bottom so that the camera of the handset 1 can completely observe the signal transduction block 20, at which point the adjusting means at the top are fixed.
Preferably, the bar code patch 15 includes one-dimensional code and/or two-dimensional code, which can rapidly transfer the reaction change of the detection area, by dripping 1 μl of blue solution on the back of the water absorption area 16 of the device, and drying at 45 ℃ in the oven, when the dripped sample completely wets the water absorption area 16, the blue dye pre-buried in the area will cover the area again, and there is a sensitive blue change.
Preferably, the water absorption area 16 and the bar code patch 15 are combined to form the concept of "0" and "1" bit stream forming the internal logic foundation of the computer, and are converted into computer coding information, so that the time when the mobile phone scans the information of the two-dimension code is used as a timing starting point, and the timing function is started, and the automatic timing function can be accurately and effectively realized.
Preferably, the diameter of the pre-treatment zone 11 of the detection consumable 3 is 2-5mm, which may be 2mm, 3mm, 4mm or 5mm, for example.
The detection zone 14 has a diameter of 2-5mm, which may be 2mm, 3mm, 4mm or 5mm, for example.
The side length of the absorbent region 16 is 2-7mm, and may be 2mm, 3mm, 4mm, 5mm, 6mm or 7mm, for example.
In the invention, the size and shape of the water absorption area 16 can directly improve the saturated absorption amount of the detection consumable material to the sample and realize the convenience of a specific reaction timing mode.
In a second aspect, the present invention provides the use of a detection device according to the first aspect for detecting biochemical and immunological indicators of a blood sample.
Preferably, the blood sample of the detection device comprises any one or a combination of at least two of serum, plasma or whole blood.
Preferably, the biochemical and immunological indicators of the detection device include Urine Protein (UPRO), acid phosphatase (ACP), albumin (ALB), ethanol (ALC), alkaline phosphatase (ALKP), glutamic pyruvic transaminase (ALT), blood Ammonia (AMON), amylase (AMYL), glutamic oxaloacetic transaminase (AST), unbound/bound bilirubin (BuBc), urea nitrogen (BUN), calcium (Ca), carbamazepine (CRBM), cholinesterase (CHE), cholesterol/high density lipoprotein (CHOL/HDL), creatine Kinase (CK), creatine kinase isozyme (CKBM), and, Chlorine (Cl), inosine (CREA), C-reactive protein (CRP), digoxin (DGXN), carbon dioxide (ECO 2), iron/total iron binding (Fe/TIBC), gamma-glutamyl transphthalase (GGT), glucose (GLU), potassium (K+), lactic Acid (LAC), lactate Dehydrogenase (LDH), lipase (LIPA), lithium (Li), magnesium (Mg), sodium (Na+), phenobarbital (PHBR), phenytoin sodium (PHYT), phosphorus (PHOS), cerebrospinal Protein (PROT), salicylic Acid (SALI), theophylline (THEO), potassium (K+), magnesium (Mg), sodium (Na+), phenobarbital (PHBR), phenytoin sodium (PHYT), phosphorus (PHOS), and combinations thereof, Total Bilirubin (TBIL), total Protein (TP), triglyceride (TRIG), uric acid (URIC), antinuclear antibodies (ANA), anti-double-stranded DNA antibodies (ds-DNA), anti-ENA antibodies, anti-cardiolipin Antibodies (ACL), anti-streptolysin "O" (ASO), immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), immunoglobulin E (IgE), prealbumin (PAB), ceruloplasmin (CER), complement 4 (C4), total complement (CH 50), beta 2 microglobulin (B2M or beta 2 MG), Carcinoembryonic antigen (CEA), alpha Fetoprotein (AFP), tumor antigen 125 (CA 125), tumor carbohydrate antigen 199 (CA 199), tumor carbohydrate antigen 153 (CA 15), tumor carbohydrate protein 72-4 (CA-72-4), cytokeratin fragment 19 (Cyfra 21-1), ferritin (Fer), chorionic gonadotrophin beta subunit, neuron Specific Enolase (NSE), squamous cell carcinoma-associated antigen (SCC), free prostate specific antigen (F-PSA), thyrotropin (TSH), triiodothyronine (T3), Thyroxine (T4), free triiodothyronine (FT 3), free thyroxine (FT 4), parathyroid hormone (PTH), thyroperoxidase (anti-TPO), thyroglobulin (TG), anti-thyroglobulin antibody (anti-TG), hepatitis A antibody (HAV-IgM), hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (HbsAb or AUSAB), hepatitis B e antigen (HbeAg), hepatitis B e antibody (HbeAb), hepatitis B CORE antibody (HbcAb or CORE), hepatitis D antibody (HDV), hepatitis E antibody (HEV-IgM), hepatitis B antibody (AUV-IgM), Any one or a combination of at least two of mycoplasma antibody (MP-IgM), tuberculosis antibody quick test (TB-AB), hepatitis C antibody (HCV), syphilis serum anti-TP-Ab or AIDS antibody (HIV).
The device provided by the invention does not limit the detection instrument and the detection items, the detection consumable can realize the record and result transmission of the detection initiation by combining with the bar code, and the device is irrelevant to the replacement and change of the detection instrument and the detection items, so that the detection of various items in a blood sample can be realized in principle.
Compared with the prior art, the invention has the following beneficial effects:
The detection device provided by the invention can judge the placement position of the detection consumable in a way of reading the bar code (one-dimensional code or two-dimensional code), can automatically judge the method for starting the detection timing, automatically and accurately determines the time of biochemical detection, realizes detection record and feedback, and is more convenient for health management of users, so that the operation of the users in the use process is more convenient, the whole detection device is not only easy to carry and miniaturize, but also has more intellectualization for record analysis of detection results and health management of the users.
Detailed Description
The technical means adopted by the invention and the effects thereof are further described below by the specific embodiments in combination with the accompanying drawings, but the invention is not limited to the examples.
Example 1 design and fabrication of detection consumables
The structure of the detection consumable material is designed through vector drawing software, and specifically comprises the following structural areas: the hydrophobic boundary 13 and the hydrophobic oleophobic boundary 10 enclose a hydrophilic area with a specific shape in the middle, three parts of the hydrophilic area are a pretreatment area 11, a detection area 14 and a water absorption area 16, the corresponding shapes are symmetrically arranged on the center or are regular polygons, such as equilateral triangles, squares, regular pentagons, regular hexagons and the like, the sizes of the detection area are respectively 2-5mm in diameter, 2-7mm in side length of the water absorption area, 5mm in diameter of the pretreatment area selected in the embodiment, 5mm in diameter of the detection area and 7mm in side length of the water absorption area, the three areas are respectively communicated by a micro-channel 12, the width of the corresponding design size of the channel is 0.5-2mm, and the width selected in the embodiment is 2mm;
The set blood sample detection demand of the whole detection consumable is less than 5 mu L, and in addition, the size and the shape of the water absorption area can directly improve the saturated adsorption quantity of the detection consumable to the sample and realize the convenience of a specific reaction timing mode;
In particular, in the design of the detection consumable, the design characteristics of the hydrophobic boundary 13 and the hydrophobic oleophobic boundary 10 are: drawing dashed lines in the areas based on the central hydrophilic channel or the reaction area, wherein the break of the dashed lines is not more than 10 mu m, the line width is optimal to be not more than 3 pounds based on the dashed lines, expanding the area of 0.5-10mm to the periphery, and drawing rectangular supporting parts, wherein the supporting parts are in solid line type, and the line width is optimal to be not more than 5 pounds;
after the design of the detection consumable is finished, the processing mode is realized according to the processes of primary processing (wax spraying printing) -primary heating (100-160 ℃ C., heating for 2-20 s) -secondary processing (ink spraying printing or brushing) -secondary heating (100-160 ℃ C., heating for 60-600 s). The specific processing steps are as follows: selecting qualitative medium-speed filter paper with the size of 210mm and 297mm as processing, selecting a wax-spraying printer for first processing, then printing the outline of a wire frame of the whole pattern on the surface of the filter paper, heating the filter paper by selecting a heating flat plate with uniform temperature distribution for heating at 110 ℃ for 10 seconds, continuously prompting the filter paper to be fed into a machine according to a paper feeding method of the ink-jet printer after the heating is finished, dripping the three-proofing finishing agent with the concentration of 0.05% into the area 10, and putting the filter paper on a heating plate again for heating after the liquid is uniformly diffused in the area 10, wherein the heating parameter is 110 ℃ and the heating time is 120 seconds;
for user operation and depositing of being convenient for, detect the consumptive material and select plastics material as the shell, as shown in fig. 4, the shell includes upper and lower part, is respectively: the thickness of the transparent plastic flat plate 4 with a flat bottom surface on the upper layer can be 0.5-10mm, an opening 7 is needed to be formed in a pretreatment area, an opening 8 in a water absorption area and a plastic flat plate of a micro groove 17 with a size diameter of less than 10mm, which corresponds to a consumable hydrophilic channel area, wherein the opening in the pretreatment area is a circular shape with a size diameter of less than 5mm, the opening in the water absorption area is a circular shape with a size diameter of less than 5mm, the micro groove is arranged at the bottom of the flat plate on the upper layer, the width of the channel is a linear channel with a size of 0.5-2 mm, the starting point of the channel is the opening in the water absorption area, and the end point of the channel is 0.5-3mm away from the edge of the opening in the pretreatment area; the lower layer is a light-tight plastic flat plate with a flat bottom surface, the thickness can be 0.5-10mm, and the external dimension is consistent with that of the upper layer flat plate;
And the reagent embedding of the detection area and the water absorption area adopts a spraying mode. When cholesterol is detected, the reagents required for the pretreatment zone are: cholesterol lipase; the detection area pre-buried reagent is cholesterol oxidase, peroxidase, 4-amino-imidacloprid and phenol; the pre-buried reagent of the water absorption area is blue dye.
After the processing is finished, the transparent two-dimensional code patch is required to be attached to the detection surface in a mode in the drawing; compared with the conventional dry biochemical test paper, the water absorption area 16 of the detection consumable is stuck with a layer of bar code adhesive tape with specific codes, the design of the detection consumable realizes the accurate control of the timing starting point of the detection process by means of the identification mode of bar codes (one-dimensional codes and two-dimensional codes), the reaction time required by the optimal detection effect is ensured, and the interference of the artificial judgment detection time on the detection result is reduced.
Example 2 Assembly of the detection device
The intelligent mobile phone 1 with the photographing function, the detector base 2 and the detection consumable 3 are assembled according to the structure shown in fig. 1 to obtain a detection device;
The detector base 2 is located below the smart phone 1 and comprises a detection window 18 located at the top, a signal transmission module 20 located at the bottom and a supporting part 19 located at the side;
The detection consumable 3 adopts a multi-layer structure compounding mode, and the following steps are from top to bottom: the structure of the upper cover plate 4, the filtering membrane 9, the detection substrate 5 and the lower cover plate 6 is shown in figure 2;
specifically, the detection consumable is located right above the signal transmission module 20 at the bottom of the detector base 2, and includes a hydrophobic boundary 13, a hydrophobic and oleophobic boundary 10, a pretreatment area 11, a detection area 14, a micro-channel 12 and a water absorption area 16, wherein a bar code patch 15 capable of transmitting changes of the detection area is adhered to the water absorption area 16, and the structure is shown in fig. 3;
When detecting the sample, the camera of the smart phone 1 shoots the detection consumable 3 right above the signal transmission module 20 through the detection window 18, and identifies the two-dimensional code.
Example 3
The detection consumable of the invention is used for cholesterol detection of different concentrations: preparing whole blood solutions with the concentration of 50mg/dL,100mg/dL,150mg/dL,200mg/dL and 250mg/dL, taking 5 mu L of the whole blood solution respectively, adding the whole blood solution into the device in the embodiment 2 of the invention for test analysis, and the detection results are shown in figure 5;
As can be seen from FIG. 5, the detection device of the present invention can well detect cholesterol in blood, and the detection result is stable and accurate.
In the examples of the present invention, cholesterol is taken as an example, and this does not mean that only cholesterol can be detected.
The applicant states that the detailed method of the present invention is illustrated by the above examples, but the present invention is not limited to the detailed method described above, i.e. it does not mean that the present invention must be practiced in dependence upon the detailed method described above. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.