CN108562747A - Enzyme exempts from fluorogenic chemiluminescence composite type enzyme mark detector - Google Patents

Abstract

The invention discloses a kind of enzymes to exempt from fluorogenic chemiluminescence composite type enzyme mark detector；It includes that seal casinghousing, pallet, motion, enzyme exempt from analytical equipment, fluorescence detection device, chemiluminescence detecting and master controller；It includes combined light source and silicon photocell plate that enzyme, which exempts from analytical equipment,；Combined light source includes multiple first light sources, multiple first light guide modules and fixed seat；Advantageous effect：Under the guide-lighting effect of the first light guide module, the light that first light source is sent out is enable to be directed at the center of silicon photocell, so that can accurately project and be received by silicon photocell across gaging hole to be checked, so that enzyme exempt to analyze it is more acurrate, simultaneously, enzyme is exempted from analytical equipment by the present invention, fluorescence detection device and chemiluminescence detecting become one, so as to be configured to the composite type enzyme mark detector with comprehensive detection function, its compared to three independently arranged enzymes exempt from analytical equipment, fluorescence detection device and chemiluminescence detecting, using more convenient, production cost can be lower.

Description

Enzyme exempts from fluorogenic chemiluminescence composite type enzyme mark detector

Technical field

The present invention relates to technical field of medical detection, in particular, being related to a kind of enzyme exempts from fluorogenic chemiluminescence composite type enzyme Mark detector.

Background technology

Immunology detection is body identification " itself " and " non-oneself " antigen, is formed naturally to autoantibody as its name suggests Immune tolerance generates " non-oneself " antigen a kind of detection of physiological function of repulsive interaction.It mainly utilizes antigen and antibody A kind of means for being detected of specific reaction, since it can utilize isotope, enzyme, chemiluminescent substance etc. to believe detection It number is amplified and shows, therefore, be commonly used for detecting the micro substances such as protein, hormone.

Immunoassay has the different detections such as enzyme-labeled immunity detection, fluorescence immunoassay detection and chemiluminescence immunoassay detection Mode.Enzyme used in enzyme-labeled immunity detection mode therein exempts from analytical equipment, generally comprises and is oppositely arranged from top to down Lamp plate and silicon photocell plate, be provided with multiple light directions light source directed downwardly on corresponding lamp plate；On corresponding silicon photocell plate then Equipped with the multiple silicon photocells opposite with multiple respective sources positions.When implementing enzyme-labeled immunity detection, then so that device needs The ELISA Plate of sample sheet is transferred by used motion between corresponding lamp plate and corresponding silicon photocell plate, so that phase The light that light source is sent out is answered to need corresponding detection hole on the ELISA Plate of sample sheet across device, thus, and respective sources The opposite silicon photocell in position then receives passed through light, to be realized using colorimetric principle and treat sample sheet It carries out enzyme and exempts from analysis detection, although such enzyme exempts from analytical equipment using extensively, it still has certain defect and deficiency.

Specifically, if the light source on lamp plate fills askew, then its light direction can be prevented from towards underface so that It cannot be directed at the center of the silicon photocell opposite with its position, in this way, then so that source light is passed through and waited for from accurately projecting Detection hole is received by silicon photocell so that it is not accurate enough to there is a problem of that enzyme exempts to analyze.

Also, although enzyme-labeled immunity detection, fluorescence immunoassay detection and chemiluminescence immunoassay detection have his own strong points, It is needed according to specific required and occasion come selection enzyme-labeled immunity detection device, fluorescence single or one by one in a particular application Immunity testing equipment or chemiluminescence immunoassay detection device are detected sample, in this way, then so that cumbersome, meanwhile, it is right It examines equipment and chemiluminescence immunoassay to examine equipment in independent enzyme-labeled immunity detection device, fluorescence immunoassay, is independently operated, Obvious cost is higher, and makes use extremely inconvenient, is not easy to proofer and one way in which is selected to carry out soon as needed Speed detection.

Thereby, it for present situation, designs a enzyme for exempting from analytical equipment with novel enzyme and exempts from fluorogenic chemiluminescence composite type Enzyme mark detector, so that detection result is good, detection quick, detection accurate, easy to detect has washability and it is at low cost etc. to make, Then it is necessary.

Invention content

It is comprehensive that a kind of enzyme provided it is an object of the invention to overcome the deficiency of the above-mentioned prior art exempts from fluorogenic chemiluminescence Box-like enzyme mark detector.

Technical solution is used by the present invention solves prior art problem：A kind of enzyme exempts from fluorogenic chemiluminescence composite type enzyme Detector is marked, including：

Seal casinghousing；

Pallet；The pallet is suitable for holding up the ELISA Plate for being equipped with sample to be checked；

Motion；The motion is set in the seal casinghousing, and is fixedly arranged on the pallet lower part, to drive The pallet is moved to move with longitudinal direction in transverse direction；

Enzyme exempts from analytical equipment；The enzyme is exempted from analytical equipment and is set in the seal casinghousing, and is located at first position；

It includes combined light source and silicon photocell plate that the enzyme, which exempts from analytical equipment,；

Vertical stand is equipped with a hanging plate along the longitudinal direction in the seal casinghousing；The combined light source is fixedly arranged on the hanging plate Side and above the pallet；The combined light source includes multiple uniformly distributed downwards in a manner of multiple rows of multiple row First light source, the first light guide module for being correspondingly arranged in multiple first light source lower parts and the side for being fixedly arranged on the hanging plate To the fixed seat of the multiple first light sources of fixation and multiple first light guide modules；

The silicon photocell plate is set to below the pallet, and opposite with the combined light source, and the silicon photoelectricity The upper surface of pond plate is evenly provided with multiple silicon photocells, the position pair of multiple silicon photocells in a manner of multiple rows of multiple row It should be opposite with multiple positions of first light guide module；

Fluorescence detection device；The fluorescence detection device is set in the seal casinghousing, and is located at the second position；

Chemiluminescence detecting；The chemiluminescence detecting is set in the seal casinghousing, and is located at third Position；

Master controller；The master controller is connect with the motion signal, to control the motion driving The pallet in transverse direction with longitudinal direction move so that the pallet be transported to the first position, the second position or The third place exempts from analytical equipment, fluorescence detection device or chemiluminescence detecting to the enzyme mark with correspondence by the enzyme The sample to be checked put on plate is analyzed and is detected.

Above technical scheme is further elaborated below：

Preferably, each first light guide module includes light-conductive optic fibre, convex lens A and filter A；

The fixed seat includes pedestal, is set to the fixed frame on the pedestal top, is multiple solid in a manner of multiple rows of multiple row It light source base, more light-guiding pillars, the filter fixed plate for being fixedly arranged on the pedestal bottom surface on the fixed frame and is fixedly arranged on The bottom plate of the filter fixed plate bottom surface；

The appearance that a correspondence houses multiple first light sources is offered in the middle part of the upper surface of multiple light source bases downwards Set chamber；One is offered downwards through the pedestal, filter in the middle part of the inner bottom surface of corresponding accommodating cavity on multiple light source bases The guide-lighting slot of fixed plate and the bottom plate；The more light-guiding pillar correspondences are solidly plugged in multiple guide-lighting slots, are led described in more Light beam axis offers a correspondence through lower face thereon and houses the accommodating of more light-conductive optic fibres along its length Slot, and the upper surface of the more light-guiding pillars corresponds to and is flush with the inner bottom surface of multiple accommodating cavities, the more light-guiding pillars Lower face correspond to be located at the pedestal in middle part；

The bottom surface of the pedestal offers multiple internal diameters and the convex lens A outer diameters upwards around multiple guide-lighting slots Compatible groove A；Multiple convex lens A correspondences are fastened in multiple groove A, and multiple convex lens A's is convex Face is downward；The bottom surface of the filter fixed plate offers multiple internal diameters and the filter upwards around multiple guide-lighting slots The compatible groove B of A outer diameters；Multiple filter A correspondences are fastened in multiple groove B, and multiple convex lens A The corresponding light-conductive optic fibre fixed in the more light-guiding pillars of relative position in center and the center of multiple filter A Central axes on.

Preferably, the fixed seat further includes the cover board that multiple corresponding lids close multiple accommodating cavities, multiple cover boards Upper surface correspond to offer so that power cord pass through cross mouth.

Preferably, the fluorescence detection device includes second light source, the second light guide module and fluorescence detector；

Second light guide module includes light guide seat, convex lens B and filter B；

The light guide seat is fixedly connected with the fixed seat, and a V-type guide lighting channel is equipped in the light guide seat；The V Type guide lighting channel include be connected to be formed V-structure into optical channel and go out optical channel；The light inlet and institute into optical channel The light-emitting window for stating out optical channel is respectively positioned on the light guide seat upper surface；The light-emitting window into optical channel with it is described go out optical channel Light inlet is mutually screwed on, and is located at the light guide seat bottom surface, and positioned at the top of the pallet；The second light source is fixed downwards At the light inlet into optical channel；The convex lens B is fixedly arranged on described into optical channel, and the convex surface of the convex lens B Downward；The filter B goes out described in being fixedly arranged in optical channel；The fluorescence detector be fixedly arranged on it is described go out optical channel light-emitting window Place.

Preferably, the center of the convex lens B is located at the light direction of the second light source and described into optical channel On axis；The center of the filter B goes out described in being located on the central axes of optical channel.

Preferably, the chemiluminescence detecting includes：

Photomultiplier；The photomultiplier is vertically installed in the seal casinghousing and positioned at the upper of the pallet Side；The lower end of the photomultiplier is incident window, and upper end is anode tap；

Photon counter；The photon counter is connected with the upper end of the photomultiplier；

Detect headgear；The movable lower end for being sheathed on the photomultiplier of the detection headgear；

Lifting gear；The lifting gear is connected with the detection headgear, to drive the detector to be sleeved on the light The lower end of electric multiplier tube slides up and down.

Preferably, the lifting gear includes：

Fixed plate；The fixed plate level is installed on the hanging plate；The photomultiplier is installed on the fixation The lower section of plate；

Elevating lever, activity is worn in the fixed plate elevating lever in the vertical direction, the detection headgear and the liter The lower end connection of bar is dropped；

Driving device, the driving device are connected with the upper end of the elevating lever, to drive the elevating lever vertically Movement, and then moved up and down by the lower end that the elevating lever drives the detector to be sleeved on the photomultiplier.

Preferably, the driving device includes：

Driving motor A；The driving motor A is installed on the upper surface of the fixed plate and connects with the master controller signal It connects, and one eccentric wheel assembly of output axis connection of the driving motor A；The upper end of the elevating lever connects a linkage board, described The lower surface of eccentric wheel assembly and the linkage board offsets, to drive the linkage by the eccentric motion of the eccentric wheel assembly Plate moves upwards；

Spring, the spring pocket be set to the elevating lever on, and the spring be compressive state, the upper end of the spring with The lower surface of the fixed plate offsets, and the upper end of the spring offsets with the detection headgear.

Preferably, two symmetrically arranged magnet are installed, the eccentric wheel assembly side is equipped on the eccentric wheel assembly One Hall sensor being connect with the master controller signal；

When one of two magnet are opposite with the Hall sensor, the Hall sensor generates inductive signal, institute State the position that master controller judges the detection headgear according to the inductive signal.

Preferably, which is characterized in that the motion includes：

Sliding rail；There are two the sliding rail is set, two sliding rails are set to the interior bottom of the seal casinghousing in transverse direction Face, and the both sides of the corresponding inner bottom surface positioned at the seal casinghousing；

Sliding seat；There are two the sliding seat is set, two sliding seats are oppositely arranged, and are corresponded in transverse direction slidably Be fixedly arranged on two sliding rails；

Grip block；The grip block is fixedly arranged on the wherein one sliding seat side；

Driving motor B；The driving motor B be set to along the longitudinal direction the seal casinghousing inner bottom surface and with the master control Device signal connection processed, and it is located at the grip block side；

Driving wheel；The driving wheel is fixedly arranged on the output shaft of the driving motor B；

Driven wheel；The driven wheel is set to the inner bottom surface of the seal casinghousing, and setting parallel with the driving wheel, And the equal length of the spacing between the driven wheel and the driving wheel and the sliding rail；

Driving wheel band；The driving wheel band set is fixed on the driving wheel and the driven wheel, and the driving wheel band presss from both sides It is fixed on the grip block；

Driving motor C；The driving motor C be fixedly arranged on along the longitudinal direction on wherein one sliding seat and with the master control Device signal connection processed；

Lead screw；One end of the lead screw is fixedly connected with the output shaft of the driving motor C, and the other end along the longitudinal direction may be used Rotation is installed on another sliding seat；

Guide rod；The guide rod is fixed on along the longitudinal direction between two sliding seats；

Feed screw nut；Feed screw nut's screw-thread fit is on the lead screw；

Sliding block；The sliding block is fixedly arranged on the feed screw nut, and is offered on the sliding block so that the guide rod is worn The pilot hole crossed；

One end of the pallet is fixedly connected on the sliding block.

The beneficial effects of the invention are as follows：

A kind of enzyme provided by the present invention exempts from fluorogenic chemiluminescence composite type enzyme mark detector, in the specific implementation, a side Face, it includes combined light source and silicon photocell plate that set enzyme, which exempts from analytical equipment,；The combined light source includes multiple with more It arranges the downward uniformly distributed first light source of mode of multiple row and is correspondingly arranged in the first leaded light of multiple first light source lower parts Module；The silicon photocell plate is opposite with the combined light source, and the upper surface of the silicon photocell plate is with the side of multiple rows of multiple row Formula is evenly provided with multiple silicon photocells, and the position of multiple silicon photocells corresponds to and multiple first light guide modules Position is opposite；Using the present invention treat sample this progress enzyme exempt from analysis when so that device needs the ELISA Plate companion of sample sheet The pallet is transferred by used motion between corresponding first light guide module and corresponding silicon photocell plate, so that The light that corresponding first light source is sent out is guided through corresponding first light guide module and to pass through device to need right on the ELISA Plate of sample sheet The detection hole answered, in this way, under the guide-lighting effect of corresponding first light guide module, i.e., so that the light that corresponding first light source is sent out Light direction towards underface so that the center of the silicon photocell opposite with its position can be directed at so that corresponding first light source The light sent out can accurately be projected and be received by corresponding silicon photocell across gaging hole to be checked so that enzyme exempt to analyze it is more acurrate, On the other hand, the present invention is not only provided with the enzyme in first position and exempts from analytical equipment, is additionally provided with the fluorescence in the second position Detection device, the chemiluminescence detecting in the third place and the motion for driving the tray motion, in this way, passing through The motion can be driven hold up need sample sheet ELISA Plate tray motion to the first position, the second position or The third place exempts from analytical equipment, fluorescence detection device or chemiluminescence detecting to the enzyme mark with correspondence by the enzyme The sample to be checked put on plate is analyzed and is detected so that detection is efficient and convenient, uses the efficient and convenient and time saving province of use Power, and proofer can also select one of which detection mode or Through Several Survey Measure to treat sample to be originally detected as needed, Make it possible to meet different detection demands, meanwhile, enzyme is exempted from analytical equipment, fluorescence detection device and chemiluminescence and examined by the present invention It surveys device to become one, so as to be configured to the composite type enzyme mark detector with comprehensive detection function, solely compared to three It erects the enzyme set and exempts from analytical equipment, fluorescence detection device and chemiluminescence detecting, occupied space meeting smaller, production cost meeting Lower, in turn, the present invention is highly practical, and use reliability is good and using effect is good.

Description of the drawings

Fig. 1 is the stereogram that enzyme of the present invention exempts from fluorogenic chemiluminescence composite type enzyme mark detector；

Fig. 2 is that enzyme of the present invention exempts from entirety of the fluorogenic chemiluminescence composite type enzyme mark detector when removing the seal casinghousing Structural schematic diagram；

Fig. 3 is the overall structure diagram one of the combined light source in the embodiment of the present invention；

Fig. 4 is the exploded view one of the combined light source in the embodiment of the present invention；

Fig. 5 is the exploded view two of the combined light source in the embodiment of the present invention；

Fig. 6 is the overall structure diagram of the fluorescence detection device in the embodiment of the present invention；

Fig. 7 is the front view sectional view of the fluorescence detection device in the embodiment of the present invention；

Fig. 8 is the overall structure diagram of the chemiluminescence detecting in the embodiment of the present invention；

Fig. 9 is in the embodiment of the present invention, the overall structure diagram at a motion structure visual angle wherein；

The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.

Drawing reference numeral：

Seal casinghousing 10；

Hanging plate 101；Holder 102；Top plate 103；Door closure 104；

Pallet 20；

ELISA Plate 30；

Detection hole 301；

Motion 40；

Sliding rail 401；Sliding seat 402；Grip block 403；Driving motor B404；Driving wheel 405；Driven wheel 406；Driving wheel band 407；Driving motor C408；Lead screw 409；Guide rod 410；Feed screw nut 411；Sliding block 412；

Enzyme exempts from analytical equipment 50；

Combined light source 51；First light source 511；First light guide module 512；Light-conductive optic fibre 5121；Convex lens A5122；Filter Mirror A5123；Fixed seat 513；Pedestal 5131；Groove A51311；Screw hole A51312；Fixed frame 5132；Light source base 5133；Accommodating cavity 51331；Guide-lighting slot 51332；Mounting hole B51333；Light-guiding pillar 5134；Storage tank 51341；Filter fixed plate 5135；Groove B51351；Screw hole B51352；Bottom plate 5136；Screw hole C51361；Cover board 5137；Cross mouth 51371；Mounting hole A51372；Silicon photoelectricity Pond plate 52；Silicon photocell 521；

Fluorescence detection device 60；

Second light source 61；Second light guide module 62；Light guide seat 621；V-type guide lighting channel 6211；Into optical channel 6211A；Go out Optical channel 6211B；Inner edge A 6212；Inner edge B6213；Recessed rank 6214；Convex lens B622；Filter B623；Fluorescence detector 63；

Chemiluminescence detecting 70；

Photomultiplier 71；Photon counter 72；Detect headgear 73；Lifting gear 74；Fixed plate 741；Elevating lever 742； Driving device 743；Driving motor A7431；Eccentric wheel assembly 7432；Linkage board 7433；

Specific implementation mode

Below with reference to the drawings and the specific embodiments technical solution that the present invention will be described in detail, to become apparent from, intuitively Understand the invention essence of the present invention.

In conjunction with shown in Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6, Fig. 7, Fig. 8 and Fig. 9；

A kind of enzyme provided by the present invention exempts from fluorogenic chemiluminescence composite type enzyme mark detector, including：

Seal casinghousing 10；The seal casinghousing 10 is for providing a closed darkroom, in order to the optics of sample to be checked Detection.

Pallet 20；The pallet 20 is suitable for holding up the ELISA Plate 30 for being equipped with sample to be checked；The upper surface of the ELISA Plate 30 Uniformly offer multiple detection holes 301 to device sample to be checked downwards in a manner of multiple rows of multiple row；

Motion 40；The motion 40 is set in the seal casinghousing 10, and is fixedly arranged under the pallet 20 Portion, to drive the pallet 20 to be moved in transverse direction with longitudinal direction；

Enzyme exempts from analytical equipment 50；The enzyme is exempted from analytical equipment 50 and is set in the seal casinghousing 10, and is located at first It sets；

It includes combined light source 51 and silicon photocell plate 52 that the enzyme, which exempts from analytical equipment 50,；

Vertical stand is equipped with a hanging plate 101 along the longitudinal direction in the seal casinghousing 10；The combined light source 51 is fixedly arranged on The side of the hanging plate 101 and positioned at the top of the pallet 20；The combined light source 51 includes multiple sides with multiple rows of multiple row The downward uniformly distributed first light source 511 of formula, the first light guide module for being correspondingly arranged in multiple 511 lower parts of the first light source 512 and be fixedly arranged on the hanging plate 101 side to the multiple first light sources 511 of fixation and multiple first guide-lighting moulds The fixed seat 513 of block 512；

The silicon photocell plate 52 is set to 20 lower section of the pallet, and opposite and described with the combined light source 51 The upper surface of silicon photocell plate 52 is evenly provided with multiple silicon photocells 521, multiple silicon light in a manner of multiple rows of multiple row The position of battery 521 corresponds to opposite with the position of multiple first light guide modules 512；

Fluorescence detection device 60；The fluorescence detection device 60 is set in the seal casinghousing 10, and is located at second It sets；

Chemiluminescence detecting 70；The chemiluminescence detecting 70 is set in the seal casinghousing 10, and position In the third place；

Herein, that is to say, that the enzyme exempts from analytical equipment 50, fluorescence detection device 60 and chemiluminescence detecting 70 then It is set to three different positions in the seal casinghousing 10.

Master controller；The master controller is connect with 40 signal of the motion, to control the motion 40 The pallet 20 is driven to be moved in transverse direction with longitudinal direction, so that the pallet 20 is transported to the first position, the Two positions or the third place exempt from analytical equipment 50, fluorescence detection device 60 or chemiluminescence detection dress with corresponding by the enzyme The sample to be checked put on 70 pairs of ELISA Plates 30 is set to be analyzed and detected.

Based on described above, it is clear that, a kind of enzyme provided by the present invention exempts from fluorogenic chemiluminescence composite type enzyme Detector is marked, in the specific implementation：

On the one hand, it includes combined light source 51 and silicon photocell plate 52 to exempt from analytical equipment 50 because of set enzyme；Described group Box-like light source 51 includes multiple first light sources 511 uniformly distributed downwards in a manner of multiple rows of multiple row and is correspondingly arranged in multiple institutes State the first light guide module 512 of 511 lower part of first light source；The silicon photocell plate 52 is opposite with the combined light source 51, institute The upper surface for stating silicon photocell plate 52 is evenly provided with multiple silicon photocells 521, multiple silicon in a manner of multiple rows of multiple row The position of photocell 521 corresponds to opposite with the position of multiple first light guide modules 512；Sample sheet is being treated using the present invention When progress enzyme exempts from analysis so that the ELISA Plate 30 that device needs sample sheet passes through used motion in company with the pallet 20 40 are transferred between corresponding first light guide module 512 and corresponding silicon photocell plate 52, so that corresponding first light source 511 was sent out Light guides through corresponding first light guide module 512 and needs corresponding detection hole 301 on the ELISA Plate 30 of sample sheet across device, In this way, under the guide-lighting effect of corresponding first light guide module 512, i.e., so that the light that corresponding first light source 511 is sent out goes out Light direction is towards underface so that can be directed at the center of the silicon photocell 521 opposite with its position so that corresponding first light source 511 light sent out can accurately be projected and be received by the opposite corresponding silicon photocell 521 in position across gaging hole 301 to be checked, So that multiple silicon photocells 521 using colorimetric principle when realizing that sample enzyme to be checked exempts from analysis detection, it is more accurate that enzyme exempts from analysis Really.

On the other hand, it because the present invention is not only provided with the enzyme in first position and exempts from analytical equipment 50, is additionally provided in the The fluorescence detection device 60 of two positions, the chemiluminescence detecting 70 in the third place and drive the pallet 20 move Motion 40, in this way, can be driven the fortune of pallet 20 for holding up the ELISA Plate 30 for needing sample sheet by the motion 40 It moves to the first position, the second position or the third place, analytical equipment 50, fluorescence detection device is exempted from by the enzyme with correspondence 60 or chemiluminescence detecting 70 sample to be checked put on the ELISA Plate 30 is analyzed and is detected so that detection It is efficient and convenient, time saving and energy saving using efficient and convenient and use, and proofer can also select one of which detection mode as needed Or Through Several Survey Measure is treated sample and is originally detected, enabling meets different detection demands.

Meanwhile enzyme is exempted from analytical equipment 50, fluorescence detection device 60 and chemiluminescence detecting 70 and is integrated by the present invention One, so as to be configured to the composite type enzyme mark detector with comprehensive detection function, compared to three independently arranged enzymes are exempted from Analytical equipment 50, fluorescence detection device 60 and chemiluminescence detecting 70, occupied space meeting smaller, production cost can be lower.

In turn, the present invention is highly practical, and use reliability is good and using effect is good.

It is emphasized that in the technical scheme, each first light guide module 512 includes light-conductive optic fibre 5121, convex lens A5122 and filter A5123；

The fixed seat 513 include pedestal 5131, be set to 5131 top of the pedestal with its integrally formed fixation Frame 5132, multiple light source bases 5133 being fixedly arranged in a manner of multiple rows of multiple row on the fixed frame 5132, more light-guiding pillars 5134, It is fixedly arranged on the filter fixed plate 5135 of 5131 bottom surface of the pedestal and is fixedly arranged on the bottom of 5135 bottom surface of filter fixed plate Plate 5136；

Preferably, in the technical scheme, the bottom surface of the pedestal 5131 uniformly offers multiple screw hole A51312； The bottom surface of the filter fixed plate 5135, which uniformly offers, multiple is through to end face thereon and correspondence and multiple screw holes The opposite screw hole B51352 in the positions A51312；The bottom surface of the bottom plate 5136, which uniformly offers, multiple is through to end face thereon And the corresponding screw hole C51361 opposite with multiple positions the screw hole B51352；

In this way, the bottom plate 5136 and the filter fixed plate 5135 then by screw in the opposite screw hole C51361 in position, The clamping screw of screw hole B51352 and screw hole A51312 and be fixedly connected with the pedestal 5131, stationarity is good between its While, dismounting each other is also convenient for so that convenient for safeguarding.

Preferably, in the technical scheme, a pair is offered downwards in the middle part of the upper surface of multiple light source bases 5133 The accommodating cavity 51331 of multiple first light sources 511 should be housed；In corresponding accommodating cavity 51331 on multiple light source bases 5133 Inner bottom surface in the middle part of downwards offer one through the pedestal 5131, filter fixed plate 5135 and the bottom plate 5136 leaded light Slot 51332；The more correspondences of the light-guiding pillar 5134 are solidly plugged in multiple guide-lighting slots 51332, the more light-guiding pillars 5134 Axis offers a correspondence through lower face thereon and houses the accommodating of more light-conductive optic fibres 5121 along its length Slot 51341, and the upper surface of the more light-guiding pillars 5134 corresponds to and is flush with the inner bottom surface of multiple accommodating cavities 51331, The lower face of the more light-guiding pillars 5134, which corresponds to, is located at middle part in the pedestal 5131；

The bottom surface of the pedestal 5131 around multiple guide-lighting slots 51332 offer upwards multiple internal diameters with it is described convex The compatible groove A51311 of lens A5122 outer diameters；Multiple convex lens A5122 correspondences are fastened in multiple grooves In A51311, and the convex surface of multiple convex lens A5122 is downward；The bottom surface of the filter fixed plate 5135 is around multiple The leaded light slot 51332 offers multiple internal diameters groove B50351 compatible with the filter A5123 outer diameters upwards；Multiple institutes It states filter A5123 correspondences to be fastened in multiple groove B50351, and the center of multiple convex lens A5122 and multiple institutes State the corresponding light-conductive optic fibre 5121 fixed in the more light-guiding pillars 5134 of relative position in center of filter A5123 Central axes on.

In this way, i.e. multiple first light sources 511 of toilet device are askew in assembly fashionable dress, multiple first light sources 511 are sent out The light gone out can be directed at by the more piece light-conductive optic fibres 5121 opposite with its position opposite with its position multiple well The convex lens A5122 carries out optically focused, forms point light source to enable good, is formed by point light source by corresponding position It after the filter A5123 carries out the filtering of light wave, and can make the light wave that can effectively filter out unwanted wavelength, then make Obtaining the light wave of required wavelength can accurately be projected in the form of point light source and pass through gaging hole 301 to be checked by the silicon photoelectricity of relative position Pond 521 receives so that enzyme exempts from that analytical effect is more preferable and so that enzyme exempts to analyze more accurate.

To which using effect of the invention can effectively improve.

Also, in the technical scheme, the fixed seat 513 further includes that multiple corresponding lids close multiple accommodating cavities 51331 cover board 5137, the upper surface of multiple cover boards 5137 correspond to the mouth 51371 excessively offered so that power cord passes through.

Preferably, in the technical scheme, the upper surface of multiple cover boards 5137 offers a mounting hole respectively A51372；The upper surface of multiple light source bases 5133 offers the mounting hole on the cover board 5137 with relative position respectively The positions A51372 are relatively fixed hole B51333；The mounting hole of the multiple cover boards 5137 by screwing in relative position The fastening bolt of A51372 and the mounting hole B51333 and correspondence be fixedly connected with multiple light source bases 5133.

Preferably, in the present embodiment, the fluorescence detection device 60 include second light source 61, the second light guide module 62 and Fluorescence detector 63；

Second light guide module 62 includes light guide seat 621, convex lens B622 and filter B623；

The light guide seat 621 is fixedly connected with the fixed seat 513, and it is logical that a V-type leaded light is equipped in the light guide seat 621 Road 6211；The V-type guide lighting channel 6211 include be connected to be formed V-structure into optical channel 6211A and go out optical channel 6211B；The light inlet into optical channel 6211A and it is described go out optical channel 6211B light-emitting window be respectively positioned on the light guide seat 621 Upper surface；The light-emitting window into optical channel 6211A with it is described go out optical channel 6211B light inlet be mutually screwed on, and positioned at described 621 bottom surface of light guide seat, and positioned at the top of the pallet 20；The second light source 61 is fixedly arranged on downwards described into optical channel At the light inlet of 6211A；The convex lens B622 is fixedly arranged on described into optical channel 6211A, and the convex lens B622's is convex Down；The filter B623 goes out described in being fixedly arranged in optical channel 6211B；It is logical that the fluorescence detector 63 is fixedly arranged on the light extraction At the light-emitting window of road 6211B.

Preferably, the center of the convex lens B622 is located at the light direction of the second light source 61 and described into optical channel On the central axes of 6211A；The center of the filter B623 goes out described in being located on the central axes of optical channel 6211B.

Based on described above, when treating sample this progress fluoroscopic examination using the present invention so that device needs sample sheet ELISA Plate 30 be transferred under corresponding second light guide module 62 by used motion 40 in company with the pallet 20 Side, so that the light that corresponding second light source 61 is sent out guides through corresponding second light guide module 62 and needs sample sheet across device Corresponding detection hole 301 on ELISA Plate 30.

In this way, the light that is sent out of the second light source 61 is after the convex lens B622 of relative position carries out optically focused, Form point light source so that energy of light source is big, is formed by point light source and passes through the light-emitting window into optical channel 6211A accurate It is accurately projected on the sample to be checked in the detection hole 301 of ELISA Plate 30 afterwards so that the sample energy to be checked in detection hole 301 Fluorescence is generated well, and generated fluorescence can be reflected onto on the filter B623 of corresponding position, so as to carry out light wave Filtering so that the light wave that can effectively filter out other unwanted wavelength then enables the light wave of required wavelength Go out the light-emitting window of optical channel 6211B described in accurately passing through and be sent to the detection that the fluorescence detector 63 carries out fluorescence, makes It obtains and coordinates fluorescence detector 63, incident light source energy bigger that sample to be checked is excited to generate fluorescence effect relative to traditional y-type optical fiber Fruit is more preferable, and structure is simpler and makes cost lower.

In turn, using effect of the invention can be further enhanced.

Herein, you need to add is that, in the specific implementation, it is described go out optical channel 6211B in, the filter B623 with It is described go out optical channel 6211B light-emitting window between be fixedly provided with convex surface convex lens C (not shown) directed downwardly；

In this way, generated fluorescence is reflected onto on the filter B623 of corresponding position, so as to the filtering of light wave is carried out, So that after effectively filtering out the light waves of other unwanted wavelength, the light wave of required wavelength can pass through the institute of corresponding position It is not shown to state convex lens C) carry out light direction correction so that the light light-emitting angle of injection will not dissociate and intersect so that Can be formed well with it is multiple it is described go out the parallel directional light in the central axes of optical channel 6211B so that can be accurate after injection Pass through it is described go out optical channel 6211B light-emitting window and accurately sent to the fluorescence detector 63 and carry out the detection of fluorescence, Fluoroscopic examination effect is enable to reach more preferable.

Herein, it should be noted that in the specific implementation, it is described into a circle is installed in optical channel 6211A holding up The inner edge A6212 of the convex lens B622, it is described go out optical channel 6211B in the middle part of be installed with a circle to hold up the filter The inner edge B6213 of B623, it is described go out optical channel 6211B top internal diameter slightly larger than it is described go out optical channel 6211B middle part Internal diameter, in this way, i.e. so that its screw-on place is formed as a recessed rank 6214, the convex lens C is then held up by the recessed rank 6214 and is fixed.

Preferably, in the specific implementation, the outer diameter of the convex lens B622 is mutually fitted with the internal diameter into optical channel 6211A Match, the outer diameter of the filter B623 with it is described go out optical channel 6211B middle part internal diameter be adapted, the outer diameter of the convex lens C with It is described go out optical channel 6211B upper inner diameter be adapted；And the upper end of the inner edge A6212 downwards concave shape become with it is described The compatible concave surface A in lower part convex surface of convex lens B622；The upper end of the recessed rank 6214 downwards concave shape become with it is described convex The compatible concave surface C in lower part convex surface of lens C；In this way, i.e. so that the convex lens B622 and convex lens C placements are firm.

Further, in the present embodiment, the chemiluminescence detecting 70 includes：

Photomultiplier 71；The photomultiplier 71 is vertically installed in the seal casinghousing 10 and is located at the pallet 20 top；The lower end of the photomultiplier 71 is incident window, and upper end is anode tap；

Photon counter 72；The photon counter 72 is connected with the upper end of the photomultiplier 71；The capsul The inner bottom surface of body 10 is equipped with one to prop the holder 102 of the hanging plate 101, and 102 top horizontal of the holder is equipped with a top Plate 103, the photon counter 72 are fixedly arranged on 103 bottom surface of the top plate.

Detect headgear 73；The movable lower end for being sheathed on the photomultiplier 71 of the detection headgear 73；

Lifting gear 74；The lifting gear 74 is connected with the detection headgear 73, to drive the detection headgear 73 It is slided up and down in the lower end of the photomultiplier 71.

In this way, when the motion 40 drives the pallet 20 to move to the lower section of the photomultiplier 71, it can The detection headgear 73 is driven to decline by the lifting gear 74 so that the detection headgear 73 and the enzyme on the pallet 20 Target 30 is bonded, at this point, the incident window of the photomultiplier 71 can receive the inspection of the ELISA Plate 30 on the pallet 20 The light emitted by the sample to be checked of institute's device in gaging hole 301, and be amplified by the photomultiplier 71, and by described Photon counter 72 carries out photon counting, that is, realizes institute's device in the detection hole 301 to the ELISA Plate 30 on the pallet 20 Sample to be checked carries out chemiluminescence detection.

In the specific implementation, the lifting gear 74 includes：

Fixed plate 741；The fixed plate 741 is horizontal to be installed on the hanging plate 101；The photomultiplier 71 is installed In the lower section of the fixed plate 741；

Elevating lever 742, activity is worn in the fixed plate 741 elevating lever 742 in the vertical direction, the detection headgear 73 connect with the lower end of the elevating lever 742；Preferably, the elevating lever 742 is set as two, two elevating levers 742 Vertical and parallel is threaded through in the fixed plate 741.

Driving device 743, the driving device 743 are connected with the upper end of the elevating lever 742, to drive the lifting Bar 742 vertically moves, and then drives the detection headgear 73 under the photomultiplier 71 by the elevating lever 742 End moves up and down.

In the specific implementation, the driving device 743 includes：

Driving motor A7431；The driving motor A7431 be installed on the upper surface of the fixed plate 741 and with the master Controller signals connect, and one eccentric wheel assembly 7432 of output axis connection of the driving motor A7431；The elevating lever 742 Upper end level one linkage board 7433 of connection, the eccentric wheel assembly 7432 and the lower surface of the linkage board 7433 offset, To drive the linkage board 7433 to move upwards by the eccentric motion of the eccentric wheel assembly 7432；

Spring (not shown), the spring (not shown) is sheathed on the elevating lever 742, and the spring (not shown) For compressive state, the upper end and the lower surface of the fixed plate 741 of the spring (not shown) offset, the spring (not shown) Upper end offset with the detection headgear 73.

Based on described above, when treating sample this progress chemiluminescence detection using the present invention so that device has to be checked The ELISA Plate 30 of sample is transferred by used motion 40 under corresponding detection headgear 73 in company with the pallet 20 Side, so that device needs 301 face of corresponding detection hole on the ELISA Plate 30 of sample sheet and accordingly detects headgear 73.

During carrying out chemiluminescence detection, when the motion 40 drives the pallet 20 to move to the light It when under electric multiplier tube 71, then drives the elevating lever 742 to move downward by the driving device 743, and then drives the inspection Gauge head set 73 slides down to desired height position in 71 lower end of the photomultiplier, described in the desired height position 73 lower part of detection headgear can just fit with the ELISA Plate 30 held up on the pallet 20, on the ELISA Plate 30 Light emitted by the sample to be checked can enter to the incident window of the photomultiplier 71 by the detection headgear 73.

Herein, also need to show is, on the one hand, when the driving motor A7431 works, the driving motor A7431 Output shaft can drive the eccentric wheel assembly 7432 rotate, can be by the linkage when the eccentric wheel assembly 7432 rotates Plate 7433 is raised (extreme higher position), the linkage board 7433 be further driven to the elevating lever 742 and the detection headgear 73 to Upper movement, at this point, the detection headgear 73 can be detached with the ELISA Plate 30 of lower section.On the other hand, the spring (not shown) In confined state, so providing elastic reaction, which can force the detection headgear 73 and the elevating lever 742 move downward.That is, the driving motor A7431 do not drive the eccentric wheel assembly 7432 rotate and to institute When stating linkage board 7433 and generating upward active force, the elastic acting force of the spring (not shown) can make the detector Set 73 and the elevating lever 742 drop to extreme lower position (spring (not shown) plays the role of elastic reset), at this point, the detection Headgear 73 can be bonded with the ELISA Plate 30 of lower section；And when the driving motor works, drive the eccentric wheel assembly 7432 When rotating and generating upward active force to the linkage board 7433, the linkage board 7433 is raised into (extreme higher position), it is described Linkage board 7433 is further driven to the elevating lever 742 and the detection headgear 73 moves upwards, spring (not shown) quilt Further compression.

To in conjunction with the driving motor A7431 and the spring (not shown) alternating action, you can realize the lifting The driving that bar 742 and the detection headgear 73 move up and down.

So, on the one hand, in detection process, be bonded come shape with the ELISA Plate 30 after being declined by the detection headgear At sealing path channels, so that the light emitted by the sample to be checked in the detection hole 301 of ELISA Plate 30 is by accordingly sealing light path Channel can be conducted to the incident window of the photomultiplier 71 so that the accuracy and precision of detection can be effectively improved, On the other hand, then it makes it possible to that the photomultiplier 71 is effectively avoided directly to contact with ELISA Plate 30 and to corresponding photomultiplier transit Pipe 71 causes to damage.

In turn, using effect of the invention can be improved further.

Supplement is needed further exist for, in the specific implementation, two is equipped on the eccentric wheel assembly 7432 and is symmetrical arranged Magnet, 7432 side of the eccentric wheel assembly be equipped with a Hall sensor being connect with the master controller signal；

When one of two magnet are opposite with the Hall sensor, the Hall sensor generates inductive signal, institute State the position that master controller judges the detection headgear 73 according to the inductive signal.

That is, passing through the induction of the magnet and the Hall sensor, you can detect the eccentric wheel assembly 7432 state, when the eccentric wheel assembly 7432 is jack-up state, then the linkage board 7433 drives the elevating lever 742 Rise, corresponding, the detection headgear 73 is then to be located at higher position, that is to say and is in and 30 discrete state of ELISA Plate.And When the eccentric wheel assembly 7432 is not jack up state, then so that the elevating lever under the action of spring (not shown) 742 decline, corresponding, and the detection headgear 73 is then to be located at lower position, be that is to say in the shape being bonded with ELISA Plate 30 State.

In this way, by the detection of the position (or state) to the detection headgear 73, may be implemented to carry out detection process Accurately control, and then realize continuous detection operation and improve its working efficiency.

And in all of above embodiment, in the specific implementation, the motion 40 includes the present invention：

Sliding rail 401；There are two the sliding rail 401 is set, two sliding rails 401 are set to the capsul in transverse direction The inner bottom surface of body 10, and the both sides of the corresponding inner bottom surface positioned at the seal casinghousing 10；

Sliding seat 402；There are two the sliding seat 402 is set, two sliding seats 402 are oppositely arranged, and in transverse direction Correspondence is slidably fixedly arranged on two sliding rails 401；

Grip block 403；The grip block 403 is fixedly arranged on 402 side of wherein one sliding seat；

Driving motor B404；The driving motor B404 be set to along the longitudinal direction 10 inner bottom surface of the seal casinghousing and with The master controller signal connection, and it is located at 403 side of the grip block；

Driving wheel 405；The driving wheel 405 is fixedly arranged on the output shaft of the driving motor B404；

Driven wheel 406；The driven wheel 406 is set to the inner bottom surface of the seal casinghousing 10, and with the driving wheel 405 Parallel setting, and the equal length of the spacing between the driven wheel 406 and the driving wheel 405 and the sliding rail 401；

Driving wheel band 407；407 sets of the driving wheel band is fixed on the driving wheel 405 and the driven wheel 406, and institute Driving wheel band 407 is stated to be clamped on the grip block 403；

In this way, when the driving motor B404 works, the driving wheel 405 can drive 407 turns of the driving wheel band Dynamic, the driving wheel band 407 then can drive the grip block 403 to move in transverse direction.

Driving motor C408；The driving motor C408 is fixedly arranged on wherein one sliding seat 402 simultaneously along the longitudinal direction It is connect with the master controller signal；

Lead screw 409；One end of the lead screw 409 is fixedly connected with the output shaft of the driving motor C408, other end edge Longitudinal direction is rotatably installed on another sliding seat 402；

Guide rod 410；The guide rod 410 is fixed on along the longitudinal direction between two sliding seats 402；

Feed screw nut 411；411 screw-thread fit of the feed screw nut is on the lead screw 409；

Sliding block 412；The sliding block 412 is fixedly arranged on the feed screw nut 411, and offered on the sliding block 412 so that The pilot hole that the guide rod 410 passes through；

In this way, when the driving motor C408 works, the lead screw 409 can drive the sliding block 412 to be led along described It slides, i.e., is moved along the longitudinal direction to bar 410.

And one end of the pallet 20 is fixedly connected on the sliding block 412.And along longitudinal direction because of the driving motor C408 Direction is fixedly arranged on wherein one sliding seat 402 and is connect with the master controller signal；One end of the lead screw 409 and institute The output shaft for stating driving motor C408 is fixedly connected, and the other end is rotatably installed on another sliding seat along the longitudinal direction On 402；

In this way, realizing that the pallet 20, can under the driving of the driving motor B404 and the driving motor C408 Correspondence is moved and is moved along the longitudinal direction in transverse direction so that it can be transferred into the first position, the second position and institute It states the third place and corresponds to and analytical equipment 50, fluorescence detection device 60 or chemiluminescence detecting 70 are exempted to institute by the enzyme The sample to be checked put on the ELISA Plate 30 that pallet 20 is held up is stated to be analyzed and detected.

In conjunction with described above, it is clear that, in the motion 40, horizontal direction is using transmission V belt translation dress Set the transmission pallet 20 to move, longitudinal direction transmits the pallet 20 using spindle gear and moves, on the one hand, i.e. so that Entire motion 40 is simple and clear so that easy to disassemble and maintenance, on the other hand, motion stabilization is reliable, kinematic error It is small so that the pallet 20 can be accurately sent to specified coordinate position and in case inspection on the ELISA Plate 30 held up thereon The detection of the sample to be checked of institute's device in gaging hole 301.

In turn, using effect of the invention can reach best.

And preferably, in the technical scheme, a side end face lower part of the seal casinghousing 10 is equipped with a door closure 104, institute It states door closure 104 to remove out of the seal casinghousing 10 suitable for the pallet 20, in this way, before detection, the fortune can be passed through Motivation structure 40 drives the pallet 20 to be moved to outside the seal casinghousing 10 from the door closure 104, then will be loaded with and wait for sample This ELISA Plate 30 is placed on the pallet 20, is then held up the pallet 20 and thereon by the motion 40 ELISA Plate 30 be moved to inside the seal casinghousing 10, finally close the door closure 104, you can carry out the optics of sample to be checked Analysis and detection open the door closure 104, by the motion 40 by the pallet after analysis detection experiment 20 and the ELISA Plate 30 that is held up thereon be moved to that the seal casinghousing 10 is outer, in this way, being conveniently operated, facilitating will detect The device that the ELISA Plate 30 finished unloads and loads onto next batch needs the ELISA Plate 30 of sample sheet.

Other embodiment etc., here, not illustrating one by one.

In conclusion overall structure of the present invention is simple, easily implement, easy to operate, highly practical, specificity is strong so that this hair It is bright necessarily to have good market popularization value, the present invention be very welcome, it can effectively be popularized.

The foregoing is merely the preferred embodiment of the present invention, are not intended to limit the range of the patent protection of the present invention, all It is the transformation of equivalent structure or equivalent process made by the specification and accompanying drawing content using the present invention, directly or indirectly uses In other related technical areas, it is included within the scope of the present invention.

Claims (10)

1. a kind of enzyme exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that including：

Seal casinghousing；

Pallet；The pallet is suitable for holding up the ELISA Plate for being equipped with sample to be checked；

Motion；The motion is set in the seal casinghousing, and is fixedly arranged on the pallet lower part, to drive Pallet is stated to move with longitudinal direction in transverse direction；

Enzyme exempts from analytical equipment；The enzyme is exempted from analytical equipment and is set in the seal casinghousing, and is located at first position；

It includes combined light source and silicon photocell plate that the enzyme, which exempts from analytical equipment,；

Vertical stand is equipped with a hanging plate along the longitudinal direction in the seal casinghousing；The combined light source is fixedly arranged on the one of the hanging plate Side and above the pallet；The combined light source includes multiple in a manner of multiple rows of multiple row downwards uniformly distributed first Light source, the first light guide module for being correspondingly arranged in multiple first light source lower parts and the side for being fixedly arranged on the hanging plate to The fixed seat of the fixed multiple first light sources and multiple first light guide modules；

The silicon photocell plate is set to below the pallet, and opposite with the combined light source, and the silicon photocell plate Upper surface be evenly provided with multiple silicon photocells in a manner of multiple rows of multiple row, the positions of multiple silicon photocells correspond to The position of multiple first light guide modules is opposite；

Fluorescence detection device；The fluorescence detection device is set in the seal casinghousing, and is located at the second position；

Chemiluminescence detecting；The chemiluminescence detecting is set in the seal casinghousing, and is located at the third place；

Master controller；The master controller is connect with the motion signal, to control described in the motion driving Pallet is moved with longitudinal direction in transverse direction, so that the pallet is transported to the first position, the second position or third Analytical equipment, fluorescence detection device or chemiluminescence detecting are exempted on the ELISA Plate in position with correspondence by the enzyme The sample to be checked put is analyzed and is detected.

2. enzyme according to claim 1 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that each described First light guide module includes light-conductive optic fibre, convex lens A and filter A；

The fixed seat includes pedestal, be set to the fixed frame on the pedestal top, multiple is fixedly arranged in a manner of multiple rows of multiple row It light source base, more light-guiding pillars, the filter fixed plate for being fixedly arranged on the pedestal bottom surface on the fixed frame and is fixedly arranged on described The bottom plate of filter fixed plate bottom surface；

The accommodating cavity that a correspondence houses multiple first light sources is offered in the middle part of the upper surface of multiple light source bases downwards； One is offered downwards through the pedestal, filter fixed plate in the middle part of the inner bottom surface of corresponding accommodating cavity on multiple light source bases And the guide-lighting slot of the bottom plate；The more light-guiding pillar correspondences are solidly plugged in multiple guide-lighting slots, in the more light-guiding pillars Axis offers the storage tank that a correspondence through lower face thereon houses the more light-conductive optic fibres along its length, and more The upper surface of light-guiding pillar described in root is corresponded to be flush with the inner bottom surface of multiple accommodating cavities, the lower face of the more light-guiding pillars It is corresponding to be located at middle part in the pedestal；

The bottom surface of the pedestal offers multiple internal diameters around multiple guide-lighting slots and is mutually fitted with the convex lens A outer diameters upwards The groove A matched；Multiple convex lens A correspondences are fastened in multiple groove A, and the convex surface of multiple convex lens A is equal Downward；The bottom surface of the filter fixed plate is offered around multiple guide-lighting slots outside multiple internal diameters and the filter A upwards The compatible groove B of diameter；Multiple filter A correspondences are fastened in multiple groove B, and in multiple convex lens A The corresponding light-conductive optic fibre fixed in the more light-guiding pillars of relative position in the center of the heart and multiple filter A On central axes.

3. enzyme according to claim 2 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that the fixation Seat further includes the cover board that multiple corresponding lids close multiple accommodating cavities, and the upper surface of multiple cover boards, which corresponds to, to be offered so that electricity What source line passed through crosses mouth.

4. enzyme according to claim 2 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that the fluorescence Detection device includes second light source, the second light guide module and fluorescence detector；

Second light guide module includes light guide seat, convex lens B and filter B；

The light guide seat is fixedly connected with the fixed seat, and a V-type guide lighting channel is equipped in the light guide seat；The V-type is led Optical channel include be connected to be formed V-structure into optical channel and go out optical channel；The light inlet into optical channel and it is described go out The light-emitting window of optical channel is respectively positioned on the light guide seat upper surface；The light-emitting window into optical channel with it is described go out optical channel entering light Mouth is mutually screwed on, and is located at the light guide seat bottom surface, and positioned at the top of the pallet；The second light source is fixedly arranged on downwards institute It states at the light inlet of optical channel；The convex lens B is fixedly arranged on described into optical channel, and the convex surface of the convex lens B is downward； The filter B goes out described in being fixedly arranged in optical channel；The fluorescence detector goes out described in being fixedly arranged at the light-emitting window of optical channel.

5. enzyme according to claim 4 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that the convex lens The center of mirror B is located on light direction and the central axes into optical channel of the second light source；The centre bit of the filter B In it is described go out optical channel central axes on.

6. enzyme according to claim 1 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that the chemistry Luminescence detection apparatus includes：

Photomultiplier；The photomultiplier is vertically installed on the top in the seal casinghousing and positioned at the pallet；Institute The lower end for stating photomultiplier is incident window, and upper end is anode tap；

Photon counter；The photon counter is connected with the upper end of the photomultiplier；

Detect headgear；The movable lower end for being sheathed on the photomultiplier of the detection headgear；

Lifting gear；The lifting gear is connected with the detection headgear, to drive the detector to be sleeved on the photoelectricity times The lower end for increasing pipe slides up and down.

7. enzyme according to claim 6 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that the lifting Device includes：

Fixed plate；The fixed plate level is installed on the hanging plate；The photomultiplier is installed on the fixed plate Lower section；

Elevating lever, activity is worn in the fixed plate elevating lever in the vertical direction, the detection headgear and the elevating lever Lower end connection；

Driving device, the driving device are connected with the upper end of the elevating lever, to drive the elevating lever vertically to move, And then it is moved up and down by the lower end that the elevating lever drives the detector to be sleeved on the photomultiplier.

8. enzyme according to claim 7 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that the driving Device includes：

Driving motor A；The driving motor A is installed on the upper surface of the fixed plate and is connect with the master controller signal, And one eccentric wheel assembly of output axis connection of the driving motor A；The upper end of the elevating lever connects a linkage board, the bias The lower surface of wheel assembly and the linkage board offsets, with by the eccentric motion of the eccentric wheel assembly drive the linkage board to Upper movement；

Spring, the spring pocket be set to the elevating lever on, and the spring be compressive state, the upper end of the spring with it is described The lower surface of fixed plate offsets, and the upper end of the spring offsets with the detection headgear.

9. enzyme according to claim 8 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that the bias Two symmetrically arranged magnet are installed, the eccentric wheel assembly side is connect equipped with one with the master controller signal on wheel assembly Hall sensor；

When one of two magnet are opposite with the Hall sensor, the Hall sensor generates inductive signal, the master Controller judges the position of the detection headgear according to the inductive signal.

10. enzyme according to claim 1 exempts from fluorogenic chemiluminescence composite type enzyme mark detector, which is characterized in that its feature It is, the motion includes：

Sliding rail；There are two the sliding rail is set, two sliding rails are set to the inner bottom surface of the seal casinghousing in transverse direction, and The both sides of the corresponding inner bottom surface positioned at the seal casinghousing；

Sliding seat；There are two the sliding seat is set, two sliding seats are oppositely arranged, and corresponding slidable solid in transverse direction On two sliding rails；

Grip block；The grip block is fixedly arranged on the wherein one sliding seat side；

Driving motor B；The driving motor B be set to along the longitudinal direction the seal casinghousing inner bottom surface and with the master controller Signal connects, and is located at the grip block side；

Driving wheel；The driving wheel is fixedly arranged on the output shaft of the driving motor B；

Driven wheel；The driven wheel is set to the inner bottom surface of the seal casinghousing, and setting parallel with the driving wheel, and institute State the equal length of the spacing and the sliding rail between driven wheel and the driving wheel；

Driving wheel band；The driving wheel band set is fixed on the driving wheel and the driven wheel, and the driving wheel band is clamped on On the grip block；

Driving motor C；The driving motor C be fixedly arranged on along the longitudinal direction on wherein one sliding seat and with the master controller Signal connects；

Lead screw；One end of the lead screw is fixedly connected with the output shaft of the driving motor C, and the other end is rotatable along the longitudinal direction Be installed on another sliding seat；

Guide rod；The guide rod is fixed on along the longitudinal direction between two sliding seats；

Feed screw nut；Feed screw nut's screw-thread fit is on the lead screw；

Sliding block；The sliding block is fixedly arranged on the feed screw nut, and is offered on the sliding block so that the guide rod passed through Pilot hole；

One end of the pallet is fixedly connected on the sliding block.