CN108541921A - A kind of method that low temperature enzymolysis process prepares degummed flax seeds bud powder - Google Patents
A kind of method that low temperature enzymolysis process prepares degummed flax seeds bud powder Download PDFInfo
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- CN108541921A CN108541921A CN201810217693.8A CN201810217693A CN108541921A CN 108541921 A CN108541921 A CN 108541921A CN 201810217693 A CN201810217693 A CN 201810217693A CN 108541921 A CN108541921 A CN 108541921A
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- linseed
- degumming
- enzymolysis
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- bud powder
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
- A23L25/30—Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
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Abstract
The invention discloses a kind of methods that low temperature enzymolysis process prepares degummed flax seeds bud powder.The present invention provides a kind of methods of linseed degumming, include the following steps：Linseed is subjected to low temperature enzymolysis with alkaline pectase, realizes degumming.The present invention carries out enzymolysis method for degumming, optimal enzymolysis process parameter to linseed under cryogenic with alkaline pectase：It is 8.3 to digest pH value, and enzyme concentration is 1.7 × 103U/g, and hydrolysis temperature is 36.3 DEG C, enzymolysis time 3.8h, and the predicted value of degumming rate is 94.3%, and the predicted value of germination percentage is 98.5%.Verification test is carried out using the process conditions of above-mentioned optimization, its degumming rate of gained is 94.1%, germination percentage 98.7%, and error is little with theoretical expectation values difference less than 5%.
Description
Technical field
The present invention relates to the sides that biotechnology more particularly to a kind of low temperature enzymolysis process prepare degummed flax seeds bud powder
Method.
Background technology
Linseed is a kind of characteristic oil plant originating in the cold and cool highlands in northwest China and northeast, is rich in grease and α-flax
(27wt% is left for acid, flax lignan (1wt%~4wt%), flaxseed gum (8wt%~14w t%), flax seed edible fiber
It is right) etc. multiple biological activities functional component.There is these active constituents apparent health-care effect and pharmacological activity, modern medicine to grind
Studying carefully, which proves that linseed has, adjusts blood fat, adjusts blood glucose, anticancer, antiallergy, anti-aging, improvement memory and skin barrier function etc.
Many physiological activity and pharmacological activity, linseed are a kind of natural work(with comprehensive process value of exploiting and utilizing and application potential
It can property raw-food material.Therefore, around the development and utilization of linseed, and for producing with bioactivity or functional new
Type product has become one of the hot spot studied at this stage.
Flax bud powder is at present high-end one of product in flax seed product processing.The study found that plant seed is sprouted
When sending out young shoot, many active constituents contained in plant seed have different degrees of raising.Domestic and foreign scholars studies have shown that
After linseed sends out young shoot, the substances such as leukotrienes and lignan therein increase, and the flex seed dimension life germinateed
Plain E contents increase 966% than the flex seed of crushing, and Vitamin C content increases 925% than the flex seed of crushing, dimension
Raw element B6 contents increase 483%, and folate content increases 272%, and lipase content increases 322%, and content of lignin increases
Add 14%, insoluble fibre reduces 49%, and phytic acid reduces 49%.The linseed that germinates is direct-edible, and nutritional ingredient is held
Absorption easy to digest, and develop germination flax seed product and promote the development of industry to be of great significance to elongating its industrial chain.
The germination of general seed is easier to control, as long as after spending rest period, meeting certain temperature, moisture and air
It can germinate.Common germinator is made of the babinet equipped with air-breather, spraying device and temperature regulating device, and seed is spread
In babinet inner support plate, setting can sprout germination suitable for temperature, air and the moisture of seed germination.However, linseed and its
His seed is different, contains one layer of flax glue on linseed surface layer, accounts for 10% or so of seed gross mass, it has very strong water suction
Property, and one is cohered into after absorbing water, extensive linseed germination effect is influenced, and the linseed bud powder containing glue can hinder human body
Its nutrition is digested and assimilated, therefore the colloid of seed must be detached before germination.And it is existing for industrializing degumming
Method is using patent ZL00133515.4 as representative, and it is raw material that this method, which is by linseed, through hot water extraction, separation, dehydration
The linseed of degumming is obtained afterwards.Since this method is carried out in the case that temperature is higher, subsequent hair can not be carried out after degumming
Bud.
Invention content
It is an object of the present invention to provide a kind of methods of linseed degumming.
Method provided by the invention, includes the following steps：Linseed is subjected to low temperature enzymolysis with alkaline pectase, is realized de-
Glue.
In the above method, the addition proportioning of the alkaline pectase and the linseed is 1.7 × 103U：1g.
In the above method, the temperature of the low temperature enzymolysis is 36.3 DEG C.
In the above method, the time of the low temperature enzymolysis is 3.8h.
In the above method, the pH value needed for the low temperature enzymolysis is 8.3.
In the above method, the linseed exists in the form of linseed and water mix；
And/or the solid-liquid ratio of the linseed and the water is 1g：7ml.
Further include following steps after the enzymolysis in the above method：Product filters after digesting, and collects and is less than or equal to 400
Purpose filtered fluid；Again by the filtered fluid successively through vacuum concentration, the ethanol water precipitation of volumn concentration 70% and nothing
Water-ethanol washs, and collects precipitation, as linseed after degumming.
Linseed is also the scope of protection of the invention after the degumming prepared by the above method.
Application of the linseed in preparing linseed bud powder is also the scope of protection of the invention after above-mentioned degumming.
Another object of the present invention is to provide a kind of method preparing linseed bud powder.
Method provided by the invention, includes the following steps：
1) linseed after degumming is prepared according to the method described above；
2) impregnate linseed after the degumming for 10% aqueous sodium hypochlorite solution through germination, mass percentage successively,
Cleaning, low temperature drying and milling, obtain linseed bud powder.
This experiment carries out degumming using alkaline pectase to linseed, and with linseed degumming rate, linseed germinates after degumming
Rate is measurement index.On the basis of experiment of single factor, obtained under different process conditions by response surface analysis, pectase
To linseed carry out degumming effect influence, then by the linseed after degumming temperature be 25 DEG C, humidity 90%, germinating time
(optimum process condition that linseed germination is had determined that by the research work of early period) germinates under conditions of 72h,
Study influence of the different degumming tech conditions to linseed germination percentage, and find out optimal degumming tech parameter, and with acidity
Pectase has carried out contrast experiment, and corresponding process parameters and certain theoretical foundation are provided for the processing of subsequent linseed.
The experiment proves that the present invention carries out enzymolysis method for degumming to linseed under cryogenic with alkaline pectase,
Optimal enzymolysis process parameter：It is 8.3 to digest pH value, and enzyme concentration is 1.7 × 103U/g, and hydrolysis temperature is 36.3 DEG C, enzymolysis time
Predicted value for 3.8h, degumming rate is 94.3%, and the predicted value of germination percentage is 98.5%.Using above-mentioned optimization process conditions into
Row verification test, its degumming rate of gained are 94.1%, and germination percentage 98.7%, error is less than 5%, not with theoretical expectation values difference
Greatly.
Description of the drawings
Fig. 1 is the flow chart that low temperature enzymolysis process prepares degummed flax seeds bud powder.
Specific implementation mode
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Linseed in following embodiments is that (variety name is black for industrial crops research institute of Heilongjiang Academy of Agricultural Sciences self-fertile kind
Asia is 20)；
Alkaline pectase (enzymatic activity 7000u/g, Shanghai source leaf biology Co., Ltd L18S6L3572), absolute ethyl alcohol,
Other chemical reagent are that analysis is pure.
The gel content assay method of raw material linseed in following embodiments is as follows：
The linseed m1 for weighing certain mass, with solid-liquid ratio 1g：The material-water ratio of 20ml, 100 DEG C of boiling 60min, silk mistake
It filters (400 mesh), the linseed filtered out ibid operates again, is repeated 4 times, and 4 gained filtered fluid mixing are concentrated in vacuo, 70%
Ethanol solution is precipitated, and is repeated 3 times, then is washed 2 times with absolute ethyl alcohol, up to no Precipitation.60 DEG C of items of gained sediment
1h is dried under part, then drying to constant weight under the conditions of 105 DEG C, cooling, weigh m2.
Colloid quality m1/ linseed quality m2 × 100% of the gel content (%) of linseed=drying to constant weight.
Degumming rate assay method in following embodiments is as follows：
The filtered fluid that the linseed handled under different condition obtains after silk (400 mesh) filtering, is concentrated in vacuo, and method is same
Dry biomass M is gone up to obtain,
Degumming rate (%)=dry biomass M/ (linseed gel content × linseed quality) × 100%.
The assay method of linseed germination percentage in following embodiments is as follows：
The linseed after degumming 100 is taken, and gets germinating bed ready, usually makees germination container with the germination plate of disinfection,
Sterilized filter paper (3-4 layers) filter paper is added to and suctions water paving in container.The linseed of experiment is first dipped to clean water
Water is suctioned, taking-up is slightly filtered dry again by group respectively seed pendulum on germinating bed, and grain is not contacted with grain to be advisable.It to be marked on germination container
Bright tested number, and put in climatic chamber, it is 25 DEG C in temperature, humidity 90%, germinating time carries out under conditions of 72h
Germination.
Linseed sum × 100% of germination percentage (%)=proteins in flax seeds germinative number/every part
The method that embodiment 1, low temperature enzymolysis process prepare degummed flax seeds bud powder is established
Fig. 1 is the flow chart that low temperature enzymolysis process prepares degummed flax seeds bud powder.
One, the method for enzymolysis method for degumming technique
1, enzymolysis method for degumming
It weighs every part of 30g of linseed to be placed in triangular flask, 3 Duplicate Samples of every group of experiment, with solid-liquid ratio 1g：Water is added in 7ml,
Add different gradient amount (X shown in table 12) alkaline pectase, empirically require to adjust pH value (X1), it is placed on sky after sealing
Setting experiment required temperature (X in gas concussion and cultivate case3), time (X4), it is cultivated.
After culture, cultured products are taken out, the filtered fluid obtained after silk (400 mesh) filtering is concentrated in vacuo (vacuum
Spend 0.075MPa, 65 DEG C of temperature, volume concentration to original 25%-30%), collect concentrate；Be added again into concentrate etc.
70% ethanol water of amount amount is precipitated (the static 2h of room temperature), and the filtering of 400 mesh silks is repeated 3 times, collects precipitation；Again
Precipitation is washed with absolute ethyl alcohol 2 times, up to no Precipitation；It is degummed flax seeds glue that 400 mesh silks, which filter gained sediment,
Body.
Above-mentioned sediment is dried into 1h under the conditions of 60 DEG C, then drying to constant weight under the conditions of 105 DEG C, it is cooling, it weighs；It presses
The above method measures the degumming rate under different experimental conditions.
Table 1 is the different experiments factor of enzymolysis method for degumming
As a result such as the following table 2 and table 3：
2 testing program of table and test result
The recurrence of 3 degumming rate of table and the results of analysis of variance
" * " indicates significant difference (P ＜ 0.05)；" * * " indicates difference extremely significantly (P ＜ 0.01).
According to the test result in table 2, data analysis is carried out by SAS9.0 softwares, establishes the Quadratic response of degumming rate
Regression model is：
As can be seen from Table 3：Regression model p value is 0.0001, returns item extremely significantly (P ＜ 0.01), and the quasi- item of mistake is not notable,
And model R2=94.84%, R2Adj=91.41%, illustrate that gained regression equation can be preferably fitted with test result,
Linear relationship is notable between independent variable and response.
The recurrence of 4 germination percentage of table and the results of analysis of variance
" * " indicates significant difference (P ＜ 0.05)；" * * " indicates difference extremely significantly (P ＜ 0.01).
According to the test result in table 2, data analysis is carried out by SAS9.0 softwares, establishes the Quadratic response of germination percentage
Regression model is：
As can be seen from Table 4：Regression model p value is 0.0001, returns pole significantly (P ＜ 0.01), and the quasi- item of mistake is not notable,
And model R2=91.73%, R2Adj=86.22%, illustrate that gained regression equation can be preferably fitted with test result,
Linear relationship is notable between independent variable and response.
The above results can be seen that optimal enzymolysis process parameter：It is 8.3 to digest pH value, and enzyme concentration is 1.7 × 103U/g,
Hydrolysis temperature is 36.3 DEG C, enzymolysis time 3.8h, and the predicted value of degumming rate is 94.3%, and the predicted value of germination percentage is
98.5%.
Two, the making of linseed bud powder
Linseed after the degumming obtained under above-mentioned 1 optimal enzymolysis process parameter is put into special germinating box and is germinateed, is sent out
The control of bud temperature is in control at 25 DEG C, and relative humidity control is 90%, germinating time 72h, the linseed to have been germinateed.
It is again clear with water after 10% aqueous sodium hypochlorite solution immersion 10min by the linseed mass percentage to have germinateed
Wash, low temperature drying (23 DEG C or so of temperature, drying condition are dried to 8% or less moisture) be milled afterwards (120 mesh of powder) packaging.
Embodiment 2, low temperature enzymolysis process prepare degummed flax seeds bud powder
One, enzymolysis method for degumming
1, enzymolysis method for degumming
It weighs linseed 30g to be placed in triangular flask, with solid-liquid ratio 1g：Water is added in 7ml, adds 1.7 × 103U/g alkalinity
Pectase adjusts pH value 8.3, and 36.3 DEG C of required temperature of setting experiment in air concussion incubator is placed on after sealing, carries out (rotating speed
150r/min) cultivate 3.8h.
After culture, cultured products are taken out, the filtered fluid obtained after silk (400 mesh) filtering is concentrated in vacuo (vacuum
Spend 0.075MPa, 65 DEG C of temperature, volume concentration to original 25%-30%), collect concentrate；Be added again into concentrate etc.
70% ethanol water of amount is precipitated (the static 2h of room temperature), and without centrifugation, the filtering of 400 mesh silks is repeated 3 times, collects
Precipitation；Precipitation is washed with absolute ethyl alcohol 2 times, up to no Precipitation, 400 mesh silks filtering gained sediment is degumming Asia again
Numb seed colloid.
2, degumming rate is measured
Above-mentioned sediment is dried into 1h under the conditions of 60 DEG C, then drying to constant weight under the conditions of 105 DEG C, it is cooling, it weighs；It presses
The above method measures degumming rate, and as a result degumming rate is 94.1%, almost the same with predicted value.
3, germination percentage is measured
Linseed germination percentage after detection degumming according to the method described above, as a result germination percentage is 98.7%, with predicted value basic one
It causes.
Two, the making of linseed bud powder
Linseed after the degumming obtained under above-mentioned 1 optimal enzymolysis process parameter is put into special germinating box and is germinateed, is sent out
The control of bud temperature is in control at 25 DEG C, and relative humidity control is 90%, germinating time 72h, the linseed to have been germinateed.
It is again that 10% aqueous sodium hypochlorite solution impregnates 10min, then uses water by the linseed mass percentage to have germinateed
Cleaning, milling (120 mesh of powder) is packed afterwards for low temperature drying (23 DEG C or so, dry to 8% or less moisture).
Comparative example, low temperature enzymolysis process prepare degummed flax seeds bud powder
It is almost the same with embodiment 2, it is unique the difference is that alkaline pectase is replaced with acid pectase (enzyme activity
10000u/g, Jiangsu Ruiyang Biological Technology Co., Ltd., 9012-54-8).
As a result the degumming rate of linseed is 83.3% after degumming, and linseed germination percentage is 89.5% after degumming.
The above results show that the degumming effect of acid pectase does not have alkaline pectin enzyme effect good.
Claims (10)
1. a kind of method of linseed degumming, includes the following steps：Linseed is subjected to low temperature enzymolysis with alkaline pectase, is realized
Degumming.
2. according to the method described in claim 1, it is characterized in that：The addition of the alkaline pectase and the linseed matches
It is 1.7 × 103U：1g.
3. method according to claim 1 or 2, it is characterised in that：The temperature of the low temperature enzymolysis is 36.3 DEG C.
4. according to any method in claim 1-3, it is characterised in that：The time of the low temperature enzymolysis is 3.8h.
5. method according to any one of claims 1-4, it is characterised in that：PH value needed for the low temperature enzymolysis is 8.3.
6. according to any method in claim 1-5, it is characterised in that：The linseed is mixed with linseed and water
Form exists；
And/or the solid-liquid ratio of the linseed and the water is 1g：7ml.
7. according to any method in claim 1-6, it is characterised in that：Further include following steps after the enzymolysis：It will
Product filters after enzymolysis, collects the filtered fluid less than or equal to 400 mesh；Again by the filtered fluid successively through vacuum concentration, volume hundred
Ethanol water precipitation and the absolute ethyl alcohol washing for dividing content 70%, collect and precipitate, as linseed after degumming.
8. by linseed after the degumming of any the method preparation in claim 1-7.
9. application of the linseed in preparing linseed bud powder after degumming.
10. a kind of method preparing linseed bud powder, includes the following steps：
1) linseed after degumming is prepared according to any the method in claim 1-7；
2) linseed after the degumming is impregnated through germination, mass percentage for 10% aqueous sodium hypochlorite solution successively, is cleaned,
Low temperature drying and milling obtain linseed bud powder.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110295212A (en) * | 2019-07-18 | 2019-10-01 | 黑龙江省农业科学院经济作物研究所 | A kind of method that fermentation of Aspergillus niger legal system takes flaxseed meal polypeptide |
| CN110463523A (en) * | 2019-07-16 | 2019-11-19 | 华南理工大学 | A kind of cultural method improving the accumulation of flax shoot vegetable lignan |
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| CN101316520A (en) * | 2005-09-06 | 2008-12-03 | 绿色生物公司 | Flax plumelet containing no mucilage, its byproduct and production and application |
| CN102206874A (en) * | 2010-03-30 | 2011-10-05 | 上海康地恩生物科技有限公司 | Degumming method of flax and enzyme preparation for using in degumming |
| CN104544350A (en) * | 2015-01-21 | 2015-04-29 | 杭州卡侬生物科技有限公司 | Production method of flaxseed malt powder |
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| CN101316520A (en) * | 2005-09-06 | 2008-12-03 | 绿色生物公司 | Flax plumelet containing no mucilage, its byproduct and production and application |
| CN102206874A (en) * | 2010-03-30 | 2011-10-05 | 上海康地恩生物科技有限公司 | Degumming method of flax and enzyme preparation for using in degumming |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110463523A (en) * | 2019-07-16 | 2019-11-19 | 华南理工大学 | A kind of cultural method improving the accumulation of flax shoot vegetable lignan |
| CN110463523B (en) * | 2019-07-16 | 2021-09-21 | 华南理工大学 | Cultivation method for improving accumulation of lignans in flax sprouts |
| CN110295212A (en) * | 2019-07-18 | 2019-10-01 | 黑龙江省农业科学院经济作物研究所 | A kind of method that fermentation of Aspergillus niger legal system takes flaxseed meal polypeptide |
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