CN108531155A - A kind of microorganism oil-displacing agent and preparation method for high temperature and high salinity oil reservoir - Google Patents
A kind of microorganism oil-displacing agent and preparation method for high temperature and high salinity oil reservoir Download PDFInfo
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- CN108531155A CN108531155A CN201810447314.4A CN201810447314A CN108531155A CN 108531155 A CN108531155 A CN 108531155A CN 201810447314 A CN201810447314 A CN 201810447314A CN 108531155 A CN108531155 A CN 108531155A
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- 244000005700 microbiome Species 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 22
- 239000010842 industrial wastewater Substances 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000002351 wastewater Substances 0.000 claims abstract description 11
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 10
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 claims abstract description 10
- 150000003863 ammonium salts Chemical class 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims abstract description 8
- 230000004151 fermentation Effects 0.000 claims abstract description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000011574 phosphorus Substances 0.000 claims abstract description 6
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 238000010792 warming Methods 0.000 claims abstract description 3
- 150000003839 salts Chemical class 0.000 claims description 7
- 238000006073 displacement reaction Methods 0.000 abstract description 11
- 239000000126 substance Substances 0.000 abstract description 11
- 238000011084 recovery Methods 0.000 abstract description 8
- 241000233866 Fungi Species 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 2
- 239000003921 oil Substances 0.000 description 32
- 238000005516 engineering process Methods 0.000 description 9
- 230000000813 microbial effect Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 230000006378 damage Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000003129 oil well Substances 0.000 description 3
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000007764 o/w emulsion Substances 0.000 description 1
- 244000039328 opportunistic pathogen Species 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
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- Oil, Petroleum & Natural Gas (AREA)
- General Life Sciences & Earth Sciences (AREA)
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- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The present invention relates to a kind of microorganism oil-displacing agents and preparation method for high temperature and high salinity oil reservoir, belong to technical field of oilfield tertiary oil recovery.It is after saliferous industrial wastewater, phosphorous industrial wastewater, inorganic ammonium salt, bacillus strain, albumen and water are added to the pH value value for reconciling solution after fermentation tank, to be warming up to 40~55 DEG C and stir evenly, be again heated to 125 DEG C and sterilize 25 minutes；It is to be cooled be cooled to 40~60 DEG C after bacillus strain is added, maintain the temperature in 55~60 DEG C 48 hours postcoolings of fermenting to room temperature to get to microorganism oil-displacing agent.Tolerable 121 DEG C of the stratum high-temperature of microorganism fungus kind that the present invention is cultivated, can grow in well liquid of the salinity up to 22%；Have the advantages that preparation method is simple, specific aim and operability are strong, production cost is low, safety and environmental protection, high financial profit；And it can be using the saliferous of local manufacturing enterprises, phosphorus-containing wastewater as raw materials for production；The problem of avoiding big traditional chemical oil displacement agent dosage, of high cost, pollution environment and destroying oil product.
Description
Technical field
The present invention relates to a kind of microorganism oil-displacing agents and preparation method for high temperature and high salinity oil reservoir, belong to oil field three times
Oil recovery technique field.
Background technology
As China's high temperature, exploitation with high salt, low-permeability oilfield and some oil wells subsequently enter high water-cut stage, rich in crack
The water and oil control and improving ecology problem of low-permeability oilfield are increasingly urgent；The chemical displacement of reservoir oil technology being widely used at present, it is former
Reason is to reduce viscosity of thickened oil using chemical adjuvant such as surfactant, polymer etc., and the method improves recovery ratio significant effect,
But exist simultaneously the defects of destroying stratum, pollution environment.In order to solve the deficiency of chemical displacement of reservoir oil technology, existing people utilize micro- life
Object recovers the oil (MEOR) technology to improve water drive operating efficiency and oil recovery factor, it has also become most with prospects now adopts three times
Oil tech.
Microbial-flooding Technology is to inject specific microorganism species and nutrition to water injection well stratum, its generation is made to be conducive to
Improve the displacement of reservoir oil substance of recovery ratio（Such as gas, organic acid, surfactant, biopolymer, alcohol metabolin）, they can be dropped
The interfacial tension of low rock-oil-water system forms oil-in-water emulsion, reduces Crude viscosity, improves crude oil flow performance,
To improve oil recovery factor.Microbial Enhanced Oil Recovery is less to the destruction of environment, nontoxic, safe, will not damage
The health of staff will not damage stratum, and raw material sources are wide, economic and environment-friendly.But microorganism is carried out in these oil reservoirs
The displacement of reservoir oil, general microorganism fungus kind are restricted by ground environment, and many depth often have height in thousands of rice oil reservoir below
Temperature（90 DEG C or more）, high salinity（15% or more）, microbe oil production is restricted, and this requires strains must have heatproof, resistance to
The high-efficiency strain of two kinds of characteristics of salt, but existing microorganism fungus kind can not meet these requirements, therefore solve microorganism fungus kind and exist
The high temperature resistant on stratum, resistance to high salinity are the key that further increase Microbial-flooding Technology.On March 10th, 2011, the application
One of applicant " Changjiang University " obtained the Patent No. " 201110057596.5 " of power, it is entitled that " one kind is high for high temperature
Microbial wax control agent of salt oil well and preparation method thereof ", disclose microbial wax control agent it is raw materials used and proportioning：Nacl
：15.0% -25.0%；Inorganic ammonium salt：4.0% -8.0%；Inorganic phosphate：2.0% -4.0%；Starch：10.0%
- 20.0%；Bacillus strain：5.0% -15.0%；Water：Surplus.The microbial wax control agent has and can effectively control
The wax deposition of high temperature and high salt oil well, but in production application, for opportunistic pathogen kind based on thermophilic wax strain, multiaction is anti-clearly in pit shaft
Wax, oil displacement efficiency is bad, and produce it is raw materials used be restricted, cost of material is high, causes production cost higher；Therefore, existing
It can not large-scale production and application always.
Invention content
It is an object of the present invention in view of the above shortcomings of the prior art, a kind of strain used is provided and is mainly driven with stratum
Based on oil, the phosphorus-containing wastewater of brine waste and phosphorous chemical industry enterprise that production primary raw material is produced using local medicine intermediate, both
Production cost has been saved, and corresponding wastewater utilization can have been turned waste into wealth, environmental protection；Solve current microbial oil displacement bacterium
Kind high temperature, high salinity tolerance level are low, are not only restricted by ground environment, but also influence oil displacement efficiency, and cost of material is high,
Can not large-scale production and application problem the microorganism oil-displacing agent and preparation method for high temperature and high salinity oil reservoir.
The present invention is to realize above-mentioned purpose by the following technical solutions：
A kind of microorganism oil-displacing agent for high temperature and high salinity oil reservoir, which is characterized in that it by following weight percentage original
Material is made：
Saliferous industrial wastewater 20%~25%
Phosphorous industrial wastewater 5%~10%
Inorganic ammonium salt 5%~8%
Bacillus strain 10%~18%
Albumen 0.5%~3%
Water surplus.
The saliferous industrial wastewater is medical intermediate production waste water, salt content 18%-25%；The phosphorous industry
Waste water is that phosphorous chemical industry produces waste water, and phosphorus content is 0.5%~1%.
Further, wherein the weight percent of each raw material is：
Industrial wastewater 20%~23%
Industrial wastewater 8%~10%
Inorganic ammonium salt 6%~8%
Bacillus strain 13%~16%
Albumen 0.5%~1.5%
Water surplus.
A kind of preparation method of microorganism oil-displacing agent for high temperature and high salinity oil reservoir, which is characterized in that it include with
Lower step：
Saliferous industrial wastewater, phosphorous industrial wastewater, inorganic ammonium salt, albumen, water are added in fermentation tank by weight percentage, adjusted
The pH value of solution is solved 7.0~7.2, be warming up to 40~55 DEG C stir evenly after, be again heated to 125 DEG C and sterilize 25 minutes；It waits for cold
But 40~60 DEG C are cooled to, then is added in bacillus strain to fermentation tank by weight percentage, maintains the temperature at 55~60 DEG C
48 hours postcoolings of middle fermentation are to room temperature to get to required microorganism oil-displacing agent.
The advantageous effect of the present invention compared with prior art is：
Tolerable 121 DEG C of the ground floor height of microorganism fungus kind that the microorganism oil-displacing agent for being used for high temperature and high salinity oil reservoir is cultivated
Temperature can be grown in well liquid of the salinity up to 22%；Preparation method strong, cost with simple for process, specific aim and operability
The advantages of low, safety and environmental protection, and saliferous after capable of being produced using local manufacturing enterprises, phosphorus-containing wastewater are as raw materials for production, production cost
It is low, high financial profit；It avoids traditional chemical oil displacement agent big, of high cost using caused dosage, pollution environment and destroys oil
The problem of product.
Specific implementation mode
Embodiment 1：For producing 1000 kilograms of microorganism oil-displacing agents.
The phosphorous chemical industry life that 200 kilograms of medical intermediate production waste water, phosphorus content that salt content is 23% are 0.8% is weighed respectively
100 kilograms of waste water is produced, 80 kilograms of inorganic ammonium salt, 5 kilograms of albumen, 435 kilograms of water are reconciled the pH value of solution 7.0~7.2, risen
After temperature is stirred evenly to 40~55 DEG C, it is again heated to 125 DEG C and sterilizes 25 minutes；It is to be cooled to be cooled to 40~60 DEG C, add bud
In 180 kilograms of born of the same parents' bacillus species to fermentation tank, 48 hours postcoolings of fermenting are maintained the temperature in 55~60 DEG C to room temperature, are obtained
1000 kilograms of microorganism oil-displacing agents.
1 microorganism of table drives microbial inoculum performance indicator
2 microorganism of table drives the microbial inoculum scope of application
The 3 conventional chemical oil displacement agent scope of application of table
Compared with such as conventional chemical displacement of reservoir oil of other technology of reservoir sweep, microorganism drives the present invention it can be seen from table 1, table 2 and table 3
Oil tech has following advantage：It is adaptable, applied widely；It is at low cost, the term of validity is long；It is simple to operate (construction)；Effect is good
（Increase oil, precipitation, slow down and successively decrease, improve recovery ratio）；Good environmental protection.
The above is presently preferred embodiments of the present invention, and the example above illustrates that the substantive content not to the present invention is made
Limitation in any form, technology of the person of an ordinary skill in the technical field after having read this specification according to the present invention
Essence is to any simple modification or deformation made by the above specific implementation mode, and the technology contents possibly also with the disclosure above
The equivalent embodiment for being changed or being modified to equivalent variations, in the range of still falling within technical solution of the present invention, without departing from
The spirit and scope of the invention.
Claims (4)
1. a kind of microorganism oil-displacing agent for high temperature and high salinity oil reservoir, which is characterized in that it is by following weight percentage
Raw material is made：
Saliferous industrial wastewater 20%~25%
Phosphorous industrial wastewater 5%~10%
Inorganic ammonium salt 5%~8%
Bacillus strain 10%~18%
Albumen 0.5%~3%
Water surplus.
2. a kind of microorganism oil-displacing agent for high temperature and high salinity oil reservoir according to claim 1, it is characterised in that：Institute
The saliferous industrial wastewater stated is medical intermediate production waste water, salt content 18%-25%；The phosphorous industrial wastewater is phosphatization
Work produces waste water, and phosphorus content is 0.5%~1%.
3. a kind of microorganism oil-displacing agent for high temperature and high salinity oil reservoir according to claim 1, it is characterised in that：Its
In the weight percent of each raw material be：
Industrial wastewater 20%~23%
Industrial wastewater 8%~10%
Inorganic ammonium salt 6%~8%
Bacillus strain 13%~16%
Albumen 0.5%~1.5%
Water surplus.
4. a kind of preparation method of microorganism oil-displacing agent for high temperature and high salinity oil reservoir, which is characterized in that it is by saliferous
Industrial wastewater, phosphorous industrial wastewater, inorganic ammonium salt, albumen, water are added in fermentation tank by weight percentage, reconcile the pH of solution
Value 7.0~7.2, be warming up to 40~55 DEG C stir evenly after, be again heated to 125 DEG C and sterilize 25 minutes；It is to be cooled to be cooled to 40
~60 DEG C, then be added in bacillus strain to fermentation tank by weight percentage, it is small to maintain the temperature at fermentation 48 in 55~60 DEG C
When postcooling to room temperature to get to required microorganism oil-displacing agent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810447314.4A CN108531155A (en) | 2018-05-11 | 2018-05-11 | A kind of microorganism oil-displacing agent and preparation method for high temperature and high salinity oil reservoir |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810447314.4A CN108531155A (en) | 2018-05-11 | 2018-05-11 | A kind of microorganism oil-displacing agent and preparation method for high temperature and high salinity oil reservoir |
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| CN108531155A true CN108531155A (en) | 2018-09-14 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111440605A (en) * | 2020-03-26 | 2020-07-24 | 天津博弘化工有限责任公司 | Preparation method of microbial surface activity synergist |
| CN115449361A (en) * | 2022-09-30 | 2022-12-09 | 湖北三雄科技发展有限公司 | Microbial oil displacement agent for high-temperature high-salinity oil reservoir and preparation method thereof |
Citations (2)
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| CN102676139A (en) * | 2011-03-10 | 2012-09-19 | 长江大学 | Micro paraffin inhibitor used for high-temperature high-salt oil well and preparation method thereof |
| CN106593371A (en) * | 2016-11-14 | 2017-04-26 | 烟台智本知识产权运营管理有限公司 | Method for increasing water-flooding reservoir yield through waste water of sugar industry |
-
2018
- 2018-05-11 CN CN201810447314.4A patent/CN108531155A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676139A (en) * | 2011-03-10 | 2012-09-19 | 长江大学 | Micro paraffin inhibitor used for high-temperature high-salt oil well and preparation method thereof |
| CN106593371A (en) * | 2016-11-14 | 2017-04-26 | 烟台智本知识产权运营管理有限公司 | Method for increasing water-flooding reservoir yield through waste water of sugar industry |
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| 刘国红: ""芽孢杆菌的系统进化及其属分类学特征"", 《福建农业学报》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111440605A (en) * | 2020-03-26 | 2020-07-24 | 天津博弘化工有限责任公司 | Preparation method of microbial surface activity synergist |
| CN115449361A (en) * | 2022-09-30 | 2022-12-09 | 湖北三雄科技发展有限公司 | Microbial oil displacement agent for high-temperature high-salinity oil reservoir and preparation method thereof |
| CN115449361B (en) * | 2022-09-30 | 2023-10-10 | 湖北三雄科技发展有限公司 | Microbial oil displacement agent for high-temperature high-mineralization oil reservoir and preparation method thereof |
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Application publication date: 20180914 |