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CN108523819A - Photometric feedback fluorescence navigation endoscope system and laser power automatic adjustment method - Google Patents

Photometric feedback fluorescence navigation endoscope system and laser power automatic adjustment method Download PDF

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CN108523819A
CN108523819A CN201810227406.1A CN201810227406A CN108523819A CN 108523819 A CN108523819 A CN 108523819A CN 201810227406 A CN201810227406 A CN 201810227406A CN 108523819 A CN108523819 A CN 108523819A
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CN108523819B (en
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顾兆泰
张浠
李娜娜
王翰林
安昕
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Guangdong Oupu Mandi Technology Co ltd
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B1/00Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
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    • A61B5/0071Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters

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Abstract

The invention discloses a fluorescence navigation endoscope system based on photometric feedback and a laser power automatic adjustment method.

Description

测光反馈的荧光导航内窥镜系统及激光功率自动调整方法Photometric feedback fluorescence navigation endoscope system and laser power automatic adjustment method

技术领域technical field

本发明涉及一种荧光导航内窥镜系统,尤其涉及的是一种基于测光反馈的荧光导航内窥镜系统及激光功率自动调整方法。The invention relates to a fluorescence navigation endoscope system, in particular to a fluorescence navigation endoscope system based on photometry feedback and a laser power automatic adjustment method.

背景技术Background technique

现今越来越多内窥镜系统具备荧光标记功能,为了实现更高的荧光激发效率和获得更优的成像效果,一般会使用到激光器作为荧光激发光源,并且为保证系统整体成像效果,内窥镜前段出射的激发光功率通常比较大。但是,大多数荧光内窥镜系统设定的输出激光功率为固定值,当需要靠近组织观察细节时,由于照明面积变小,使得单位面积接收到的光功率(即辐射照度)会增大,会造成灼伤或其他光生物安全问题。Nowadays, more and more endoscope systems have the function of fluorescent marking. In order to achieve higher fluorescence excitation efficiency and obtain better imaging effects, lasers are generally used as fluorescence excitation light sources, and in order to ensure the overall imaging effect of the system, endoscopic The excitation light power emitted from the front of the mirror is usually relatively large. However, the output laser power set by most fluorescence endoscope systems is a fixed value. When it is necessary to observe details close to the tissue, the light power received per unit area (i.e. irradiance) will increase due to the smaller illumination area. Can cause burns or other photobiological safety concerns.

因此,现有技术还有待于改进和发展。Therefore, the prior art still needs to be improved and developed.

发明内容Contents of the invention

本发明的目的在于提供一种基于测光反馈的荧光导航内窥镜系统及激光功率自动调整方法,旨在解决现有的内窥镜成像系统的输出激光功率为恒定值,靠近组织观察时容易造成灼伤或其他光生物安全的问题。The purpose of the present invention is to provide a fluorescent navigation endoscope system and laser power automatic adjustment method based on photometric feedback, aiming to solve the problem that the output laser power of the existing endoscope imaging system is a constant value, and it is easy to observe close to the tissue. cause burns or other photobiological safety concerns.

本发明的技术方案如下:一种基于测光反馈的荧光导航内窥镜激光功率自动调整方法,其中,具体包括以下步骤:The technical scheme of the present invention is as follows: a method for automatically adjusting the laser power of a fluorescent navigation endoscope based on photometric feedback, which specifically includes the following steps:

步骤S1:激光器发出的激光和指导光源发出的指导光通过同一根导光束传输并耦合到内窥镜中;Step S1: The laser light emitted by the laser and the guiding light emitted by the guiding light source are transmitted through the same guide beam and coupled into the endoscope;

步骤S2:激光和指导光从内窥镜前端出射并到达被观察组织,被观察组织反射的激发光、荧光和指导光由内窥镜收集;Step S2: The laser light and guiding light are emitted from the front end of the endoscope and reach the observed tissue, and the excitation light, fluorescence and guiding light reflected by the observed tissue are collected by the endoscope;

步骤S3:激发光被滤波片过滤掉,荧光和指导光由镜头聚焦,其中,荧光透过二向色分光镜成像于相机,指导光被二向色分光镜反射,入射到光电探测器;Step S3: The excitation light is filtered out by the filter, and the fluorescence and the guide light are focused by the lens, wherein the fluorescence is imaged on the camera through the dichroic beam splitter, and the guide light is reflected by the dichroic beam splitter and incident on the photodetector;

步骤S4:光电探测器把指导光信号转换成输出电压输出到光源控制模块;Step S4: The photodetector converts the guiding light signal into an output voltage and outputs it to the light source control module;

步骤S5:光源控制模块拟合出激光器的实际输出光功率与光电探测器的输出电压的关系曲线;Step S5: the light source control module fits the relationship curve between the actual output optical power of the laser and the output voltage of the photodetector;

步骤S6:光源控制模块根据激光器的实际输出光功率与光电探测器的输出电压的关系曲线自动获得激光器的应输出光功率,然后控制激光器的实际光功率输出。Step S6: The light source control module automatically obtains the output optical power of the laser according to the relationship curve between the actual output optical power of the laser and the output voltage of the photodetector, and then controls the actual optical power output of the laser.

所述的基于测光反馈的荧光导航内窥镜激光功率自动调整方法,其中,具体地,所述步骤S5中,测试不同内窥镜前端面与被观察组织之间的距离,光电探测器对应输出不同的输出电压,得到光电探测器的输出电压和内窥镜前端面与被观察组织之间的距离的关系;设系统荧光成像所需要的激光辐射照度为,在不同内窥镜前端面与被观察组织之间的距离下,实测辐射照度E,通过调整内窥镜前端出射的激光的输出光功率,使得实测值E等于,得到内窥镜前端出射的激光的输出光功率和距离的关系;最终得到光电探测器的输出电压和内窥镜前端出射的激光的输出光功率的关系,光源控制模块根据光电探测器的输出电压和内窥镜前端出射的激光的输出光功率P的关系拟合出P-V曲线,即激光器的实际输出光功率与光电探测器的输出电压的关系曲线。The method for automatically adjusting the laser power of fluorescence navigation endoscope based on photometric feedback, wherein, specifically, in the step S5, the distance between the front end surface of different endoscopes and the observed tissue is tested, and the photodetector corresponds to Output different output voltages to obtain the relationship between the output voltage of the photodetector and the distance between the front end of the endoscope and the observed tissue; the laser irradiance required for system fluorescence imaging is , under different distances between the front end surface of the endoscope and the observed tissue, the measured irradiance E, by adjusting the output light power of the laser emitted from the front end of the endoscope, the measured value E is equal to , to obtain the relationship between the output optical power of the laser emitted from the front end of the endoscope and the distance; finally obtain the relationship between the output voltage of the photodetector and the output optical power of the laser emitted from the front end of the endoscope, and the light source control module according to the output of the photodetector The relationship between the voltage and the output optical power P of the laser emitted from the front end of the endoscope is fitted to a PV curve, that is, the relationship curve between the actual output optical power of the laser and the output voltage of the photodetector.

所述的基于测光反馈的荧光导航内窥镜激光功率自动调整方法,其中,根据内窥镜应用,设定其最远观察距离为Ncm,光电探测器的输出电压对应为VN,此时,内窥镜前端出射的激光的输出光功率为最大值PNThe method for automatically adjusting the laser power of fluorescent navigation endoscope based on photometric feedback, wherein, according to the application of the endoscope, the farthest observation distance is set to be Ncm, and the output voltage of the photodetector corresponds to V N , at this time , the output optical power of the laser emitted from the front end of the endoscope is the maximum value P N :

对于D<N,此时,根据P-V曲线,可以得到实际需要的内窥镜前端出射的激光的输出光功率P;For D<N, at this time, according to the P-V curve, the actual required output optical power P of the laser emitted from the front end of the endoscope can be obtained;

对于D>N时,内窥镜前端出射的激光的输出光功率统一为PNFor D>N, the output optical power of the laser emitted from the front end of the endoscope is uniformly P N ;

其中,D为实际内窥镜前端面与被观察组织之间的距离,N为内窥镜的最远观察距离,VN为内窥镜的最远观察距离为Ncm时光电探测器对应的输出电压,PN为内窥镜的最远观察距离为Ncm时内窥镜前端出射的激光的输出光功率,P为实际需要的内窥镜前端出射的激光的输出光功率。Among them, D is the distance between the actual front end surface of the endoscope and the observed tissue, N is the farthest observation distance of the endoscope, V N is the corresponding output of the photodetector when the farthest observation distance of the endoscope is Ncm Voltage, P N is the output light power of the laser light emitted from the front end of the endoscope when the farthest observation distance of the endoscope is Ncm, and P is the output light power of the laser light emitted from the front end of the endoscope that is actually required.

所述的基于测光反馈的荧光导航内窥镜激光功率自动调整方法,优选地,采用脉冲宽度调制的方法实现激光器的实际光功率的调整,其中,采用的脉冲调制频率优选为10kHz。The method for automatically adjusting the laser power of fluorescence navigation endoscope based on photometric feedback, preferably, adopts the method of pulse width modulation to realize the adjustment of the actual optical power of the laser, wherein the pulse modulation frequency used is preferably 10 kHz.

所述的基于测光反馈的荧光导航内窥镜激光功率自动调整方法,其中,在D<时,实际需要的内窥镜前端出射的激光的输出光功率的脉冲宽度调制占空比为P/*100%,其中,P为实际需要的内窥镜前端出射的激光的输出光功率,为内窥镜的最远观察距离为Ncm时内窥镜前端出射的激光的输出光功率。The method for automatically adjusting laser power of fluorescence navigation endoscope based on photometric feedback, wherein, in D< When , the pulse width modulation duty cycle of the output optical power of the laser output from the front end of the endoscope actually required is P/ *100%, where P is the output optical power of the laser output from the front end of the endoscope actually required, is the output light power of the laser light emitted from the front end of the endoscope when the farthest viewing distance of the endoscope is Ncm.

所述的基于测光反馈的荧光导航内窥镜激光功率自动调整方法,其中,还可以采用幅度调制等多种方式实现激光器的实际光功率的调整。In the method for automatically adjusting the laser power of the fluorescent navigation endoscope based on photometric feedback, the actual optical power adjustment of the laser can also be realized in various ways such as amplitude modulation.

一种采用如上述任一项所述的基于测光反馈的荧光导航内窥镜激光功率自动调整方法的基于测光反馈的荧光导航内窥镜系统,其中,包括激光器,指导光源,导光束,内窥镜,滤波片,透镜,二向色分光镜,相机,光电探测器,光源控制模块;其中,光电探测器的感光面位于指导光的成像面;A photometric feedback-based fluorescence navigation endoscope system that adopts the photometric feedback-based fluorescence navigation endoscope laser power automatic adjustment method as described in any one of the above, wherein, it includes a laser, a guiding light source, and a light guide, Endoscope, filter, lens, dichroic beam splitter, camera, photodetector, light source control module; wherein, the photosensitive surface of the photodetector is located on the imaging surface of the guiding light;

所述激光器发出的激光和指导光源发出的指导光通过同一根导光束传输并耦合到内窥镜中;激光和指导光从内窥镜前端出射并到达被观察组织,被观察组织反射的激发光、荧光和指导光由内窥镜收集,其中,激发光被滤波片过滤掉,荧光和指导光由镜头聚焦,其中,荧光透过二向色分光镜成像于相机,指导光被二向色分光镜反射,入射到光电探测器;光电探测器将指导光信号转换成输出电压输出到光源控制模块;The laser light emitted by the laser and the guiding light emitted by the guiding light source are transmitted through the same light guide and coupled into the endoscope; the laser light and guiding light are emitted from the front end of the endoscope and reach the tissue under observation, and the excitation light reflected by the tissue under observation , Fluorescence and guiding light are collected by the endoscope, wherein the excitation light is filtered by a filter, and the fluorescent and guiding light are focused by the lens, wherein the fluorescent light is imaged on the camera through the dichroic beam splitter, and the guiding light is dichroic Mirror reflection, incident to the photodetector; the photodetector converts the guiding light signal into an output voltage and outputs it to the light source control module;

光源控制模块根据光电探测器的输出电压和内窥镜前端出射的激光的输出光功率的关系拟合出P-V曲线;根据P-V曲线,光源控制器自动获得激光器的应输出光功率,然后控制激光器的实际光功率输出。The light source control module fits the P-V curve according to the relationship between the output voltage of the photodetector and the output optical power of the laser emitted from the front end of the endoscope; according to the P-V curve, the light source controller automatically obtains the output optical power of the laser, and then controls the output power of the laser. Actual optical power output.

所述的基于测光反馈的荧光导航内窥镜系统,所述光电探测器优选采用光电雪崩二极管。In the fluorescence navigation endoscope system based on photometric feedback, the photodetector preferably adopts a photo avalanche diode.

本发明的有益效果:本发明通过提供一种基于测光反馈的荧光导航内窥镜系统及激光功率自动调整方法,通过在光源中加入指导光,并在成像光路上加入分光镜,通过光电探测器检测进入成像光路的指导光强度,经过运算得到距离并反馈到系统,可以实时调整输出光功率,在满足荧光成像的同时,可避免组织长时间收到大功率密度激光照射,减轻激光对生物组织的伤害。Beneficial effects of the present invention: the present invention provides a fluorescent navigation endoscope system based on photometric feedback and a method for automatically adjusting laser power, by adding guiding light to the light source, and adding a spectroscope on the imaging optical path, through photoelectric detection The detector detects the intensity of the guiding light entering the imaging optical path, and the distance is obtained through calculation and fed back to the system. The output optical power can be adjusted in real time. While meeting the requirements of fluorescence imaging, it can prevent the tissue from receiving high-power density laser irradiation for a long time, and reduce the impact of the laser on the biological tissue damage.

附图说明Description of drawings

图1是本发明中基于测光反馈的荧光导航内窥镜系统的结构示意图。Fig. 1 is a schematic structural diagram of a fluorescence navigation endoscope system based on photometric feedback in the present invention.

图2是本发明中基于测光反馈的荧光导航内窥镜激光功率自动调整方法的步骤流程图。Fig. 2 is a flow chart of the steps of the method for automatically adjusting the laser power of the fluorescence navigation endoscope based on photometric feedback in the present invention.

图3是本发明中通过脉冲宽度调制调整输出光功率示意图。Fig. 3 is a schematic diagram of adjusting output optical power through pulse width modulation in the present invention.

图4是本发明中通过幅度调制调整输出光功率示意图。Fig. 4 is a schematic diagram of adjusting output optical power through amplitude modulation in the present invention.

具体实施方式Detailed ways

下面详细描述本发明的实施方式,所述实施方式的示例在附图中示出,其中自始至终相同或类似的标号表示相同或类似的元件或具有相同或类似功能的元件。下面通过参考附图描述的实施方式是示例性的,仅用于解释本发明,而不能理解为对本发明的限制。Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

在本发明的描述中,需要理解的是,术语“中心”、“纵向”、“横向”、“长度”、“宽度”、“厚度”、“上”、“下”、“前”、“后”、“左”、“右”、“竖直”、“水平”、“顶”、“底”、“内”、“外”、“顺时针”、“逆时针”等指示的方位或位置关系为基于附图所示的方位或位置关系,仅是为了便于描述本发明和简化描述,而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作,因此不能理解为对本发明的限制。此外,术语“第一”、“第二”仅用于描述目的,而不能理解为指示或暗示相对重要性或者隐含指明所指示的技术特征的数量。由此,限定有“第一”、“第二”的特征可以明示或者隐含地包括一个或者更多个所述特征。在本发明的描述中,“多个”的含义是两个或两个以上,除非另有明确具体的限定。In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", " Orientation indicated by rear, left, right, vertical, horizontal, top, bottom, inside, outside, clockwise, counterclockwise, etc. The positional relationship is based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, Therefore, it should not be construed as limiting the invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of said features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

在本发明的描述中,需要说明的是,除非另有明确的规定和限定,术语“安装”、“相连”、“连接”应做广义理解,例如,可以是固定连接,也可以是可拆卸连接,或一体地连接;可以是机械连接,也可以是电连接或可以相互通讯;可以是直接相连,也可以通过中间媒介间接相连,可以是两个元件内部的连通或两个元件的相互作用关系。对于本领域的普通技术人员而言,可以根据具体情况理解上述术语在本发明中的具体含义。In the description of the present invention, it should be noted that unless otherwise specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection or a detachable connection. Connected, or integrally connected; it can be mechanically connected, or electrically connected, or can communicate with each other; it can be directly connected, or indirectly connected through an intermediary, and it can be the internal communication of two components or the interaction of two components relation. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present invention according to specific situations.

在本发明中,除非另有明确的规定和限定,第一特征在第二特征之“上”或之“下”可以包括第一和第二特征直接接触,也可以包括第一和第二特征不是直接接触而是通过它们之间的另外的特征接触。而且,第一特征在第二特征“之上”、“上方”和“上面”包括第一特征在第二特征正上方和斜上方,或仅仅表示第一特征水平高度高于第二特征。第一特征在第二特征“之下”、“下方”和“下面”包括第一特征在第二特征正下方和斜下方,或仅仅表示第一特征水平高度小于第二特征。In the present invention, unless otherwise clearly specified and limited, a first feature being "on" or "under" a second feature may include direct contact between the first and second features, and may also include the first and second features Not in direct contact but through another characteristic contact between them. Moreover, "above", "above" and "above" the first feature on the second feature include that the first feature is directly above and obliquely above the second feature, or simply means that the first feature is horizontally higher than the second feature. "Below", "beneath" and "under" the first feature to the second feature include that the first feature is directly below and obliquely below the second feature, or simply means that the first feature has a lower level than the second feature.

下文的公开提供了许多不同的实施方式或例子用来实现本发明的不同结构。为了简化本发明的公开,下文中对特定例子的部件和设置进行描述。当然,它们仅仅为示例,并且目的不在于限制本发明。此外,本发明可以在不同例子中重复参考数字和/或参考字母,这种重复是为了简化和清楚的目的,其本身不指示所讨论各种实施方式和/或设置之间的关系。此外,本发明提供了的各种特定的工艺和材料的例子,但是本领域普通技术人员可以意识到其他工艺的应用和/或其他材料的使用。The following disclosure provides many different embodiments or examples for implementing different structures of the present invention. To simplify the disclosure of the present invention, components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit the invention. Furthermore, the present disclosure may repeat reference numerals and/or reference letters in different instances, such repetition is for simplicity and clarity and does not in itself indicate a relationship between the various embodiments and/or arrangements discussed. In addition, various specific process and material examples are provided herein, but one of ordinary skill in the art may recognize the use of other processes and/or the use of other materials.

如图1所示,一种基于测光反馈的荧光导航内窥镜系统,包括激光器1,指导光源2,导光束,3,内窥镜4,滤波片5,透镜6,二向色分光镜7,相机8,光电探测器9,光源控制模块10;其中,光电探测器9的感光面位于指导光的成像面;As shown in Figure 1, a fluorescent navigation endoscope system based on photometric feedback, including a laser 1, a guiding light source 2, a light guide 3, an endoscope 4, a filter 5, a lens 6, and a dichroic beam splitter 7. A camera 8, a photodetector 9, and a light source control module 10; wherein, the photosensitive surface of the photodetector 9 is located on the imaging surface of the guiding light;

所述激光器1发出的激光和指导光源2发出的指导光通过同一根导光束3传输并耦合到内窥镜4中;激光和指导光从内窥镜4前端出射并到达被观察组织,被观察组织反射的激发光、荧光(激光由被观察组织反射后形成激发光和荧光)和指导光由内窥镜4收集,其中,激发光被滤波片5过滤掉,荧光和指导光由镜头6聚焦,其中,荧光透过二向色分光镜7成像于相机8,指导光被二向色分光镜7反射,入射到光电探测器9;光电探测器9将指导光信号转换成输出电压输出到光源控制模块10;The laser light emitted by the laser 1 and the guiding light emitted by the guiding light source 2 are transmitted through the same light guide 3 and coupled into the endoscope 4; the laser light and the guiding light are emitted from the front end of the endoscope 4 and reach the tissue under observation. The excitation light, fluorescence reflected by the tissue (the laser is reflected by the observed tissue to form excitation light and fluorescence) and guiding light are collected by the endoscope 4, where the exciting light is filtered by the filter 5, and the fluorescent light and guiding light are focused by the lens 6 , wherein the fluorescence is imaged on the camera 8 through the dichroic beam splitter 7, the guiding light is reflected by the dichroic beam splitting mirror 7, and is incident on the photodetector 9; the photodetector 9 converts the guiding light signal into an output voltage and outputs it to the light source control module 10;

测试不同距离D(D为内窥镜4前端面与被观察组织之间的距离),光电探测器9对应输出不同的输出电压V,得到光电探测器9的输出电压V与距离D的关系;设系统荧光成像所需要的激光辐射照度为,在不同距离D下,实测辐射照度E,通过调整内窥镜4前端出射的激光的输出光功率P(内窥镜4前端出射的激光的输出光功率P与激光器1的实际输出光功率相等),使得实测值E等于,就可得到内窥镜4前端出射的激光的输出光功率P和距离D的关系;最终得到光电探测器9的输出电压V和内窥镜4前端出射的激光的输出光功率P的关系(即光电探测器9的输出电压V和激光器1的实际输出光功率的关系),光源控制模块10根据光电探测器9的输出电压V和内窥镜4前端出射的激光的输出光功率P的关系拟合出P-V曲线;这样,光电探测器9的输出电压V在系统中起到距离指导作用,将光电探测器9的输出电压V输入到光源控制器10,通过P-V曲线,光源控制器10自动获得激光器1的应输出光功率,然后控制激光器1的实际光功率输出。Test different distances D (D is the distance between the front end surface of the endoscope 4 and the observed tissue), the photodetector 9 outputs different output voltages V correspondingly, and obtain the relationship between the output voltage V of the photodetector 9 and the distance D; Assuming that the laser irradiance required for the system fluorescence imaging is , at different distances D, the actual measured irradiance E, by adjusting the output optical power P of the laser light emitted from the front end of the endoscope 4 (the output optical power P of the laser light emitted from the front end of the endoscope 4 and the actual output optical power of the laser 1 equal), so that the measured value E is equal to , the relationship between the output light power P of the laser light emitted from the front end of the endoscope 4 and the distance D can be obtained; finally the relationship between the output voltage V of the photodetector 9 and the output light power P of the laser light emitted from the front end of the endoscope 4 ( That is, the output voltage V of the photodetector 9 and the actual output optical power of the laser 1 relationship), the light source control module 10 fits a PV curve according to the relationship between the output voltage V of the photodetector 9 and the output optical power P of the laser light emitted from the front end of the endoscope 4; thus, the output voltage V of the photodetector 9 is The system acts as a distance guide, the output voltage V of the photodetector 9 is input to the light source controller 10, and the light source controller 10 automatically obtains the output optical power of the laser 1 through the PV curve , and then control the actual optical power output of laser 1.

具体地,所述光电探测器9可以采用多种光源探测结构实现。本实施例中,所述光电探测器9优选采用光电雪崩二极管。Specifically, the photodetector 9 can be realized by using various light source detection structures. In this embodiment, the photodetector 9 is preferably a photoavalanche diode.

如图2所示,一种如上述所述的基于测光反馈的荧光导航内窥镜系统的激光功率自动调整方法,具体包括以下步骤:As shown in Figure 2, a method for automatically adjusting the laser power of the fluorescent navigation endoscope system based on photometric feedback as described above, specifically includes the following steps:

步骤S1:激光器1发出的激光和指导光源2发出的指导光通过同一根导光束3传输并耦合到内窥镜4中;Step S1: the laser light emitted by the laser 1 and the guiding light emitted by the guiding light source 2 are transmitted through the same guide beam 3 and coupled into the endoscope 4;

步骤S2:激光和指导光从内窥镜4前端出射并到达被观察组织,被观察组织反射的激发光、荧光(激光由被观察组织反射后形成激发光和荧光)和指导光由内窥镜4收集;Step S2: The laser light and guiding light exit from the front end of the endoscope 4 and reach the tissue under observation, the excitation light and fluorescence reflected by the tissue under observation (the laser light is reflected by the tissue under observation to form excitation light and fluorescence) and the guiding light are collected by the endoscope 4 collection;

步骤S3:激发光被滤波片5过滤掉,荧光和指导光由镜头6聚焦,其中,荧光透过二向色分光镜7成像于相机8,指导光被二向色分光镜7反射,入射到光电探测器9;Step S3: The excitation light is filtered by the filter 5, and the fluorescence and guiding light are focused by the lens 6, wherein the fluorescent light is imaged on the camera 8 through the dichroic beam splitter 7, and the guiding light is reflected by the dichroic beam splitting mirror 7 and incident on the photodetector 9;

步骤S4:光电探测器9把指导光信号转换成输出电压输出到光源控制模块10;Step S4: The photodetector 9 converts the guiding light signal into an output voltage and outputs it to the light source control module 10;

步骤S5:光源控制模块10拟合出激光器1的实际输出光功率与光电探测器9的输出电压V的关系曲线;Step S5: The light source control module 10 fits the actual output optical power of the laser 1 Relational curve with the output voltage V of photodetector 9;

步骤S6:光源控制模块10根据激光器1的实际输出光功率与光电探测器9的输出电压V的关系曲线自动获得激光器1的应输出光功率,然后控制激光器1的实际光功率输出。Step S6: The light source control module 10 according to the actual output optical power of the laser 1 The output optical power of the laser 1 is automatically obtained from the relationship curve with the output voltage V of the photodetector 9 , and then control the actual optical power output of laser 1.

具体地,所述步骤S5中,测试不同距离D(D为内窥镜4前端面与被观察组织之间的距离),光电探测器9对应输出不同的输出电压V,从而得到光电探测器9的输出电压V与距离D的关系;设系统荧光成像所需要的激光辐射照度为,在不同距离D下,实测辐射照度E,通过调整内窥镜4前端出射的激光的输出光功率P(内窥镜4前端出射的激光的输出光功率P与激光器1的实际输出光功率相等),使得实测值E等于,就可得到内窥镜4前端出射的激光的输出光功率P和距离D的关系;最终得到光电探测器9的输出电压V和内窥镜4前端出射的激光的输出光功率P的关系(即光电探测器9的输出电压V和激光器1的实际输出光功率的关系),光源控制模块10根据光电探测器9的输出电压V和内窥镜4前端出射的激光的输出光功率P的关系拟合出P-V曲线。Specifically, in the step S5, different distances D are tested (D is the distance between the front end surface of the endoscope 4 and the observed tissue), and the photodetector 9 outputs different output voltages V correspondingly, thereby obtaining the photodetector 9 The relationship between the output voltage V and the distance D; suppose the laser irradiance required for the system fluorescence imaging is , at different distances D, the actual measured irradiance E, by adjusting the output optical power P of the laser light emitted from the front end of the endoscope 4 (the output optical power P of the laser light emitted from the front end of the endoscope 4 and the actual output optical power of the laser 1 equal), so that the measured value E is equal to , the relationship between the output light power P of the laser light emitted from the front end of the endoscope 4 and the distance D can be obtained; finally the relationship between the output voltage V of the photodetector 9 and the output light power P of the laser light emitted from the front end of the endoscope 4 ( That is, the output voltage V of the photodetector 9 and the actual output optical power of the laser 1 relationship), the light source control module 10 fits the PV curve according to the relationship between the output voltage V of the photodetector 9 and the output optical power P of the laser light emitted from the front end of the endoscope 4 .

进一步地,内窥镜4前端面与被观察组织之间的距离越近,照射面积越小,指导光辐射照度越大,从而进入到光电探测器9的信号越强。因此,通过测试不同距离D情况下,光电探测器9对应输出不同的输出电压V,就可得到光电探测器9输出电压V与距离D的关系,如下表1。Furthermore, the closer the distance between the front end surface of the endoscope 4 and the observed tissue, the smaller the illuminated area, the greater the irradiance of the guiding light radiation, and thus the stronger the signal entering the photodetector 9 . Therefore, by testing the corresponding output voltage V of the photodetector 9 under different distances D, the relationship between the output voltage V of the photodetector 9 and the distance D can be obtained, as shown in Table 1 below.

表1 光电探测器9输出电压V与距离D的关系Table 1 The relationship between the output voltage V of the photodetector 9 and the distance D

设系统荧光成像所需要的激光辐射照度为,在不同距离D下,实测辐射照度E,通过调整内窥镜4前端出射的激光的输出光功率P(内窥镜4前端出射的激光的输出光功率P与激光器1的实际输出光功率相等),使得实测值E等于,就可得到内窥镜4前端出射的激光的输出光功率P和距离D的关系,如下表2。Assuming that the laser irradiance required for the system fluorescence imaging is , at different distances D, the actual measured irradiance E, by adjusting the output optical power P of the laser light emitted from the front end of the endoscope 4 (the output optical power P of the laser light emitted from the front end of the endoscope 4 and the actual output optical power of the laser 1 equal), so that the measured value E is equal to , the relationship between the output optical power P of the laser light emitted from the front end of the endoscope 4 and the distance D can be obtained, as shown in Table 2 below.

表2 内窥镜4前端出射的激光的输出光功率P和距离D的关系Table 2 Relationship between the output optical power P and the distance D of the laser light emitted from the front end of the endoscope 4

结合表1&表2,最终得到光电探测器9的输出电压V和内窥镜4前端出射的激光的输出光功率P的关系(即光电探测器9的输出电压V和激光器1的实际输出光功率的关系),如下表3,光源控制模块10根据光电探测器9的输出电压V和内窥镜4前端出射的激光的输出光功率P的关系拟合出P-V曲线。In combination with Table 1 & Table 2, the relationship between the output voltage V of the photodetector 9 and the output optical power P of the laser light emitted from the front end of the endoscope 4 is finally obtained (that is, the output voltage V of the photodetector 9 and the actual output optical power of the laser 1 relationship), as shown in Table 3 below, the light source control module 10 fits the PV curve according to the relationship between the output voltage V of the photodetector 9 and the output optical power P of the laser light emitted from the front end of the endoscope 4 .

表3 光电探测器9的输出电压V和内窥镜4前端出射的激光的输出光功率P的关系Table 3 The relationship between the output voltage V of the photodetector 9 and the output optical power P of the laser light emitted from the front end of the endoscope 4

这样,光电探测器9的输出电压V在系统中起到距离指导作用,将光电探测器9的输出电压V输入到光源控制器10,通过P-V曲线,光源控制器10自动获得激光器1的实际输出光功率,然后控制激光器1的实际光功率输出。In this way, the output voltage V of the photodetector 9 plays a distance guiding role in the system, and the output voltage V of the photodetector 9 is input to the light source controller 10, and the light source controller 10 automatically obtains the actual output of the laser 1 through the PV curve. Optical power , and then control the actual optical power output of laser 1.

优选地,根据内窥镜应用,设定其最远观察距离为Ncm(本实施例中,所述N=12cm),光电探测器的输出电压对应为VN,此时,内窥镜前端出射的激光的输出光功率为最大值PNPreferably, according to the application of the endoscope, the farthest observation distance is set to Ncm (in this embodiment, the N=12cm), and the output voltage of the photodetector corresponds to V N , at this time, the front end of the endoscope emits The output optical power of the laser is the maximum value P N :

对于D<N,此时,根据P-V曲线,可以得到实际需要的内窥镜前端出射的激光的输出光功率P;For D<N, at this time, according to the P-V curve, the actual required output optical power P of the laser emitted from the front end of the endoscope can be obtained;

对于D>N时,内窥镜前端出射的激光的输出光功率统一为PNFor D>N, the output optical power of the laser emitted from the front end of the endoscope is uniformly P N ;

其中,D为实际内窥镜4前端面与被观察组织之间的距离,N为内窥镜4的最远观察距离,VN为内窥镜4的最远观察距离为Ncm时光电探测器9对应的输出电压,PN为内窥镜4的最远观察距离为Ncm时内窥镜4前端出射的激光的输出光功率,P为实际需要的内窥镜4前端出射的激光的输出光功率。Wherein, D is the distance between the front end surface of the actual endoscope 4 and the observed tissue, N is the farthest observation distance of the endoscope 4, V N is the photodetector when the farthest observation distance of the endoscope 4 is Ncm 9 corresponding output voltage, P N is the output light power of the laser light emitted from the front end of the endoscope 4 when the farthest observation distance of the endoscope 4 is Ncm, and P is the output light of the laser light emitted from the front end of the endoscope 4 actually required power.

具体地,本技术方案采用脉冲宽度调制的方法实现激光器1的实际光功率的调整,为了不影响摄像系统成像,脉冲调制频率为10kHz(还可以选取其他频率值)。在D<时,实际需要的内窥镜4前端出射的激光的输出光功率的脉冲宽度调制占空比为P/*100%,其中,P为实际需要的内窥镜前端出射的激光的输出光功率,为内窥镜的最远观察距离为Ncm(本实施例中,优选N为12cm)时内窥镜前端出射的激光的输出光功率,见图3。Specifically, this technical solution adopts the method of pulse width modulation to realize the adjustment of the actual optical power of the laser 1. In order not to affect the imaging of the camera system, the pulse modulation frequency is 10 kHz (other frequency values can also be selected). in D< , the pulse width modulation duty cycle of the output optical power of the laser output power emitted by the endoscope 4 front end actually required is P/ *100%, where P is the actual output power of the laser output from the front end of the endoscope, is the output optical power of the laser light emitted from the front end of the endoscope when the farthest viewing distance of the endoscope is Ncm (in this embodiment, N is preferably 12cm), see FIG. 3 .

具体地,本技术方案采用幅度调制代替脉冲宽度调制,实现激光器1的实际光功率的调整,见图4。Specifically, this technical solution adopts amplitude modulation instead of pulse width modulation to realize the adjustment of the actual optical power of the laser 1 , as shown in FIG. 4 .

本技术方案除了采用脉冲宽度调制和幅度调制的方法实现激光器1的实际光功率的调整外,还可以使用其他调制方法实现激光器1的实际光功率的调整。In addition to using pulse width modulation and amplitude modulation to adjust the actual optical power of the laser 1 in this technical solution, other modulation methods can also be used to adjust the actual optical power of the laser 1 .

本技术方案通过在光源中加入指导光,并在成像光路上加入分光镜,通过光电探测器检测进入成像光路的指导光强度,经过运算得到距离并反馈到系统,可以实时调整输出光功率,在满足荧光成像的同时,可避免组织长时间收到大功率密度激光照射,减轻激光对生物组织的伤害;在荧光内窥镜靠近组织进行观察时,由于光功率不变而照明面积变小,会导致单位面积的光功率,即辐射照度,通常成平方关系增大,但在荧光成像上并不需要太大的辐射照度,因此,在距离靠近后,光功率有下调的余量。This technical solution adds guide light to the light source, and adds a spectroscope to the imaging optical path, detects the intensity of the guiding light entering the imaging optical path through a photodetector, obtains the distance through calculation and feeds it back to the system, and can adjust the output optical power in real time. While satisfying the requirements of fluorescence imaging, it can prevent the tissue from being irradiated with high-power density laser for a long time and reduce the damage of laser to biological tissue; As a result, the optical power per unit area, that is, the irradiance, usually increases in a quadratic relationship, but it does not require too much irradiance for fluorescence imaging. Therefore, after the distance is close, the optical power has a margin for reduction.

在本说明书的描述中,参考术语“一个实施方式”、“某些实施方式”、“示意性实施方式”、“示例”、“具体示例”、或“一些示例”等的描述意指结合所述实施方式或示例描述的具体特征、结构、材料或者特点包含于本发明的至少一个实施方式或示例中。在本说明书中,对上述术语的示意性表述不一定指的是相同的实施方式或示例。而且,描述的具体特征、结构、材料或者特点可以在任何的一个或多个实施方式或示例中以合适的方式结合。In the description of this specification, reference to the terms "one embodiment", "certain embodiments", "exemplary embodiments", "examples", "specific examples", or "some examples" etc. Specific features, structures, materials, or characteristics described in the preceding embodiments or examples are included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the described specific features, structures, materials or characteristics may be combined in any suitable manner in any one or more embodiments or examples.

应当理解的是,本发明的应用不限于上述的举例,对本领域普通技术人员来说,可以根据上述说明加以改进或变换,所有这些改进和变换都应属于本发明所附权利要求的保护范围。It should be understood that the application of the present invention is not limited to the above examples, and those skilled in the art can make improvements or transformations according to the above descriptions, and all these improvements and transformations should belong to the protection scope of the appended claims of the present invention.

Claims (9)

1. a kind of based on the fluorescence navigation endoscope laser power automatic adjusting method for surveying light feedback, which is characterized in that specific packet Include following steps:
Step S1:Laser that laser is sent out and instruct what light source sent out light is instructed to transmit and be coupled to by same root light guide bundles In endoscope;
Step S2:It laser and instructs light be emitted from endoscope distal end and reaches observed tissue, the excitation of observed Tissue reflectance Light, fluorescence and light is instructed to be collected by endoscope;
Step S3:Exciting light is filtered out by filter plate, fluorescence and instructs light by lens focus, wherein fluorescence is through dichroic point Light microscopic images in camera, and light is instructed to be reflected by dichroic beamsplitter, is incident on photodetector;
Step S4:Photodetector is output to light source control module instructing optical signal to be converted into output voltage;
Step S5:Light source control module fits the pass of the reality output luminous power of laser and the output voltage of photodetector It is curve;
Step S6:Light source control module is according to the relationship of the reality output luminous power of laser and the output voltage of photodetector Curve automatically obtains the Output optical power of answering of laser, then controls the practical luminous power output of laser.
The endoscope laser power automatic adjusting method 2. the fluorescence according to claim 1 based on survey light feedback navigates, It is characterized in that, specifically, in the step S5, tests the distance between different endoscope distal end faces and observed tissue, photoelectricity Detector, which corresponds to, exports different output voltage, obtain photodetector output voltage and endoscope distal end face with observed group The relationship the distance between knitted;If the required laser emission illumination of system fluorescence imaging is, in different endoscope distal end faces Under the distance between observed tissue, radiant illumination E is surveyed, by adjusting the output light work(of the laser of endoscope distal end outgoing Rate so that measured value E is equal to, obtain the relationship of the Output optical power and distance of the laser of endoscope distal end outgoing;Final The relationship of the Output optical power for the laser being emitted to the output voltage and endoscope distal end of photodetector, light source control module root The relationship of the Output optical power P for the laser being emitted according to the output voltage and endoscope distal end of photodetector fits P-V curves, That is the relation curve of the output voltage of the reality output luminous power and photodetector of laser.
The endoscope laser power automatic adjusting method 3. the fluorescence according to claim 2 based on survey light feedback navigates, It is characterized in that, according to endoscopic applications, sets its farthest viewing distance as N cm, the output voltage of photodetector corresponds to VN, at this point, the Output optical power of the laser of endoscope distal end outgoing is maximum value PN
For D<N, at this point, according to P-V curves, the output light work(of the laser for the endoscope distal end outgoing that can be actually needed Rate P;
For D>When N, the Output optical power of the laser of endoscope distal end outgoing is unified for PN
Wherein, D is the distance between practical endoscope distal end face and observed tissue, and N is the farthest viewing distance of endoscope, VN Photodetector corresponding output voltage when for the farthest viewing distance of endoscope being N cm, PNFor endoscope it is farthest observation away from From for N cm when endoscope distal end outgoing laser Output optical power, P be actual needs endoscope distal end be emitted laser Output optical power.
The endoscope laser power automatic adjusting method 4. the fluorescence according to claim 1 based on survey light feedback navigates, It is characterized in that, the adjustment of the practical luminous power of laser is realized using the method for pulse width modulation.
The endoscope laser power automatic adjusting method 5. the fluorescence according to claim 4 based on survey light feedback navigates, It is characterized in that, the pulse modulation frequency used is 10kHz.
The endoscope laser power automatic adjusting method 6. the fluorescence according to claim 5 based on survey light feedback navigates, It is characterized in that, in D<When, the pulse width modulation of the Output optical power of the laser of the endoscope distal end outgoing of actual needs accounts for Sky is than being P/* 100%, wherein P is the Output optical power of the laser of the endoscope distal end outgoing of actual needs,It is peeped to be interior The Output optical power of the laser of endoscope distal end outgoing when the farthest viewing distance of mirror is Ncm.
The endoscope laser power automatic adjusting method 7. the fluorescence according to claim 1 based on survey light feedback navigates, It is characterized in that, the adjustment for the practical luminous power for realizing laser is modulated using amplitude.
8. a kind of use navigates endoscope laser power certainly such as claim 1-7 any one of them based on the fluorescence for surveying light feedback The fluorescence navigation endoscopic system based on survey light feedback of dynamic method of adjustment, which is characterized in that including laser, light source is instructed, Light guide bundles, endoscope, filter plate, lens, dichroic beamsplitter, camera, photodetector, light source control module;Wherein, photoelectricity The photosurface of detector is located at the imaging surface for instructing light;
Laser that the laser is sent out and instruct that light source sends out instruct light to be transmitted by same root light guide bundles and be coupled in In sight glass;It laser and instructs light be emitted from endoscope distal end and reaches observed tissue, it is the exciting light of observed Tissue reflectance, glimmering Light and light is instructed to be collected by endoscope, wherein exciting light is filtered out by filter plate, fluorescence and instructs light by lens focus, wherein Fluorescence images in camera through dichroic beamsplitter, and light is instructed to be reflected by dichroic beamsplitter, is incident on photodetector;Photoelectricity Detector will instruct optical signal to be converted into output voltage and be output to light source control module;
The Output optical power for the laser that light source control module is emitted according to the output voltage and endoscope distal end of photodetector Relationship fits P-V curves;According to P-V curves, light source controller automatically obtains the Output optical power of answering of laser, then controls The practical luminous power of laser exports.
9. according to claim 8 based on the fluorescence navigation endoscopic system for surveying light feedback, which is characterized in that the photoelectricity Detector uses photoelectricity avalanche diode.
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