CN108450463B - Synergistic application of farnesol to triazole bactericide in preventing and treating powdery mildew - Google Patents

Abstract

The invention discloses that farnesol and triazole fungicides are used for preventing and treating sugarcane smut, corn smut and black powder of other crops caused by Sporisorium and Ustilago . New uses of sterilization and synergy for diseases, etc. Farnesol and triazole fungicides have obvious synergistic effects, which can greatly reduce the use of chemical pesticides, and the control effect is obviously better than that of a single agent, and has a good application prospect.

Description

Synergistic effect of farnesol on triazole fungicides in the control of smut

technical field

The invention relates to the technical field of biological control, in particular to the synergistic effect of farnesol and triazole fungicides, and is mainly used in the prevention and treatment of sugarcane smut and corn smut, as well as sporisorium and black powder Other crops smut caused by the fungus ( Ustilago ), etc.

Background technique

Sugarcane is the main raw material of my country's sugar industry, and corn is the main food crop. They are also important feed crops and biomass energy materials. Disease infection is a serious problem that has always plagued its development.

Sugarcane smut is caused by sporisorium infection. Corn smut is caused by the fungus Ustilago . These two pathogens are two types of fungi, and they are also semi-specialized parasites. Haploids do not develop disease. They infect sugarcane, corn or other plants through sexual cooperation to form dikaryotic mycelia, resulting in decreased crop yield and quality.

Farnesol is a kind of natural product extracted from plants or microorganisms, which is environmentally friendly, safe and non-toxic. Dioxatrien-1-ol, the structural formula is as follows:

。

.

Farnesol isomers are mainly divided into trans-trans, trans-cis, cis-trans and cis-cis. Various studies have revealed that farnesol can regulate the growth and proliferation of bacteria, fungi and other microorganisms Activity.

At present, the measures adopted to control sugarcane smut and corn smut include breeding of disease-resistant varieties and chemical control. Triazole fungicides are a broad-spectrum, high-efficiency, strong systemic fungicide. Hexaconazole and difenoconazole are two representative varieties of triazole fungicides. The main chemical agents have good control effect. However, due to its long residence time in water and soil, serious pesticide residues can easily cause environmental pollution. This is also to reduce the field dosage of pharmaceuticals, and the development of synergists has a particularly prominent role.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a new application of farnesol in preventing and treating smut pathogenic bacteria.

The object of the present invention is achieved through the following technical solutions:

The invention provides the application of farnesol in the synergistic effect of triazole fungicides in preventing and controlling smut; the smut mainly includes sugarcane smut, corn smut and sputum spp. and Smut of other crops caused by smut.

In the application, specifically, the smut is sugarcane smut and corn smut.

The use concentration of the farnesol is 0.01-1 μM, preferably 0.04-0.5 μM, and it is mixed with a triazole fungicide to increase the control effect.

The triazole fungicides are difenoconazole and hexaconazole.

Preferably, difenoconazole is a 10% microemulsion, and the use concentration is 5,000 to 200,000 times of dilution.

Preferably, hexaconazole is a 30% microemulsion, and the use concentration is 100,000 to 400,000 times diluted.

More preferably, 0.15 μM farnesol and 10% difenoconazole microemulsion diluted 2×10 ⁵ times are mixed for use on the proliferation and virulence of S. cane; or, farnesol and hexaconazole are mixed The ratio of 0.2μM farnesol + diluted 4×10 ⁵ times 30% hexaconazole microemulsion;

More preferably, the mixing ratio of farnesol and difenoconazole is 0.2 μM farnesol + dilute 2×10 ⁵ times 10% difenoconazole microemulsion for corn smut.

The farnesol of the present invention is obtained by purchase, and can also be obtained by extraction from plants or microorganisms. In the present invention, the farnesol and triazole fungicides (difenoconazole and/or hexaconazole) are used in different mixing ratios to treat sugarcane smut basidiospores and corn smut basidiospores. Through the statistics of the inhibition rate, synergistic ratio and colony growth, it was determined that the mixed use of farnesol and triazole fungicides can significantly improve the bacteriostatic effect and greatly reduce the usage of triazole fungicides. Therefore, it has a good prospect as a synergist of triazole fungicides in the control of smut pathogens.

Based on the cost control of production and application in the future, the present invention provides that the farnesol and triazole fungicides are mixed and formulated to be used in the control of sugarcane smut and corn smut in a concentration range of 0.01 μM to 1 μM.

Compared with the prior art, the present invention has the following beneficial effects:

1) In the present invention, when farnesol is mixed with triazole bactericides, the effect of using a single agent is obviously better, and the bactericidal ability can be significantly improved. At present, the recommended dosage of 10% difenoconazole microemulsion in the field is to dilute 4000-10000 times, and the optimal mixing ratio for the virulence effect of black powdery mildew in the present invention is: 0.15 μM + dilution 2 × 10 ⁵ times 10 % Difenoconazole microemulsion, the dosage is reduced by 20 times, and the synergistic ratio is as high as 74 times. The recommended dosage of 30% hexaconazole suspending agent in the field is 1000-5000 times, and the optimal mixing ratio for the virulence effect of black powdery mildew is 0.2 μM + diluted 4×10 ⁵ times of hexaconazole, the dosage of the drug is reduced by 80 times, and the increase The efficiency ratio reaches 62 times.

2) Farnesol, as a natural product extracted from plants or microorganisms, has certain effects on the formation of hyphae, biofilm formation, some oxidative stress responses, and regulation of cell morphology of microorganisms such as bacteria and fungi. Compared with the traditional synergist, farnesol as a synergist is green and safe, and it can also be used by the environment when it exists naturally in the environment. Soil microbial degradation and other advantages are novel.

Description of drawings

Figure 1 shows the antibacterial effect of farnesol and difenoconazole on sugarcane smut, among which, 1: 0.05μm Farnesol, difenoconazole diluted 1×10 ⁵ times, 0.025μm Farnesol + difenoconazole diluted 2 ×10 ⁵ times; 2: 0.08 μm Farnesol, difenoconazole diluted 5 × 10 ⁴ times, 0.04 μm Farnesol + difenoconazole diluted 1 × 10 ⁵ times; 3: 0.1 μm Farnesol, difenoconazole diluted 3.34×10 ⁴ times, 0.05 μm Farnesol + Difenoconazole diluted 6.68×10 ⁴ times; 4: 0.2 μm Farnesol, Difenoconazole diluted 2×10 ⁴ times, 0.1 μm Farnesol + Difenoconazole diluted 4× 10 ⁴ times; 5: 0.3 μm Farnesol, difenoconazole diluted 1.25×10 ⁴ times, 0.15 μm Farnesol + difenoconazole diluted 2.5×10 ⁴ times; 6: 0.2 μm Farnesol, difenoconazole diluted 1 ×10 ⁴ times, 0.1 μm Farnesol + difenoconazole diluted 2 × 10 ⁴ times; 7: 0.225 μm Farnesol, difenoconazole diluted 6.67 × 10 ³ times, 0.1125 μm Farnesol + difenoconazole diluted 1.33 × 10 ⁴ times; 8: 0.25 μm Farnesol, difenoconazole diluted 5×10 ³ times, 0.125 μm Farnesol + difenoconazole diluted 1×10 ⁴ times.

Figure 2 shows the viral effect of farnesol and difenoconazole on sugarcane black powder, among which, A: CK; B: difenoconazole diluted 5000 times; C: 0.5 μM Farnesol; D: difenoconazole diluted 10000x + 0.25 μM Farnesol.

Figure 3 shows the synergistic effect of farnesol on difenoconazole on sugarcane black powder virus, in which, Dif (difenoconazole) was diluted 1×10 ⁵ times; 2: 0.4 μM Farnesol; 3: Dif (benzene difenoconazole) Difenoconazole) diluted 2×10 ⁵ times + 0.1 μM Farnesol; 4: Difenoconazole diluted 2×10 ⁵ times + 0.15 μM Farnesol; 5: Difenoconazole diluted 2× 10 ^5x + 0.2 μM Farnesol.

Figure 4 shows the optimal mixing ratio of farnesol and difenoconazole on the inhibition rate of S. cane, wherein, Dif (difenoconazole): diluted 2×10 ⁴ times; Far: 0.08μM Farnesol.

Figure 5 is a graph showing the effect of farnesol and difenoconazole mixture on corn smut, A: CK; B: Dif (difenoconazole) diluted 1×10 ⁵ times; C: 0.4 μM Farnesol ; D: 0.2 μM Farnesol+Dif (difenoconazole) diluted 2×10 ⁵ -fold.

Figure 6 is the effect of farnesol and hexaconazole mixed on sugarcane black powder virus, wherein, A: CK; B: hexaconazole diluted 2×10 ⁵ times; C: 0.2 μM Farnesol; D: 0.3 μM Farnesol; E: 0.5 μM Farnesol; F: Hexaconazole 2 × 10 ⁵ times + 0.1 μM Farnesol; G: Hexaconazole 2 × 10 ⁵ times + 0.15 μM Farnesol; H: Hexaconazole 2 × 10 ⁵ times + 0.25 μM Farnesol.

Figure 7 is the synergistic effect of farnesol on hexaconazole toxicity (sugarcane smut), wherein, 1: Hex (hexaconazole) diluted 2 × 10 ⁵ times; 2: 0.4 μM Farnesol; 3: Hex (hexaconazole) alcohol) 4 x 10 ⁵ -fold + 0.1 μM Farnesol; 4: Hex (hexaconazole) 4 x 10 ⁵ -fold + 0.15 μM Farnesol; 5: Hex (hexaconazole) 4 x 10 ⁵ -fold + 0.2 μM Farnesol .

Figure 8 shows the antibacterial effect of farnesol and hexaconazole mixture on sugarcane smut, 1: 0.08 μM farnesol, 2×10 ⁵ -fold dilution of hexaconazole, 4×10 ⁵ -fold dilution of 0.04 μM farnesol+hexaconazole; 2: 0.1 μM farnesol, diluted 2×10 ⁵ -fold with hexaconazole, 4×10 ⁵ -fold diluted with 0.05 μM farnesol+hexaconazole; 3: 0.2 μM farnesol, diluted 2×10 ⁵ -fold with hexaconazole, diluted 0.1 μM farnesol+hexaconazole 4 × 10 ⁵ times; 4: 0.3 μM farnesol, diluted 2 × 10 ⁵ times with hexaconazole, 4 × 10 ⁵ times diluted with 0.15 μM farnesol + hexaconazole; 5: 0.4 μM farnesol, diluted 2 × 10 ⁵ times with hexaconazole, 0.2 μM farnesol + hexaconazole diluted 4×10 ⁵ times; 6: 0.5 μM farnesol, hexaconazole diluted 2×10 ⁵ times, 0.25 μM farnesol+hexaconazole diluted 4×10 ⁵ times.

Detailed ways

The present invention will be further clarified by the detailed description of the specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

Test material

Farnesol was purchased from Sigma Corporation, CAS Number: 4602-84-0. The farnesol was diluted with absolute ethanol into a stock solution with a concentration of 3.98 mM for later use. The 10% difenoconazole microemulsion and the 30% hexaconazole suspending agent are both produced by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences. The tested teliospores of sugarcane smut were collected from the sugarcane smut naturally occurring in the field in Guangxi. The tested pair of sugarcane smut basidiospores WT17 and WT18 were isolated from the teliospores on the sugarcane smut. The two haploids are identified as "+" and -" respectively, they can recognize each other, and perform sexual coordination to produce diploid mycelium. U9 and U10 (basidiospores of corn smut) are laboratory-preserved strains. The culture medium used in the laboratory was YEPSA+0.05% glycerol.

Test medium

YEPSA medium: 10 g yeast extract, 20 g peptone, 20 g sucrose, 18 g agar, add deionized water to make up to 1000 mL, and then sterilize routinely. YEPS liquid medium: YEPSA medium without agar. The medium was added with 0.5% glycerol to help dissolve.

Example 1

On the basis of the preliminary test, firstly, farnesol and 10% difenoconazole microemulsion were prepared in proportion to a certain concentration gradient stock solution (farnesol: 0.05 μM, 0.08 μM, 0.1 μM, 0.2 μM, 0.3 μM) , 0.4 μM, 0.5 μM; 10% difenoconazole microemulsion (dilution): 1×10 ⁵ times, 5×10 ⁴ times, 3.34×10 ⁴ times, 2×10 ⁴ times, 1.25×10 ⁴ times, 1×10 ⁴ times, 6.67×10 ³ times, 5×10 ³ times). Pick a few basidiospores from the plate and culture them in 6 mL YEPS medium overnight as seed solution. The next day, the cells are collected by centrifugation, and the cells are resuspended in YEPS liquid medium (OD ₆₀₀ is 0.2) for use. Pipette 300 μL of mother solutions of different concentrations into 2 mL sterilized centrifuge tubes, add an equal amount of basidiospore suspension and mix well to set up a single-use group; respectively pipette 250 μL of different concentrations of farnesol mother solutions and 250 μL of azoxystrobin mother solutions and mix them in 5 mL of sterile In the bacterial centrifuge tube, add an equal volume of bacterial liquid and mix well, which is recorded as the mixing group; pipette 500 μL of YEPS into a 5 mL sterilized centrifuge tube, add an equal volume of bacterial liquid and mix well, record it as the control group, and set repeats. Then, after measuring the initial OD ₆₀₀ of each treatment with a spectrophotometer, it was placed in a shaker at 28°C and incubated at 200 rpm/min for 20 hours. After the final OD ₆₀₀ of each treatment was measured, the inhibition rate was calculated by calculating the net increase in OD ₆₀₀ . .

The experimental results show (see Figure 1), the mixed use of farnesol and difenoconazole is significantly better than the single use and the inhibitory effect of doubling the concentration is even more obvious. The optimal compound group for inhibiting S. cane is 0.025 μM farnesol + difenoconazole diluted 2×10 ⁵ times, and its inhibitory effect reaches more than 99%, while the inhibition rate of 0.05 μM farnesol is only 6%, the inhibition rate of difenoconazole diluted 1×10 ⁵ times was 25%. Only when difenoconazole was diluted to 5×10 ³ times, the inhibition rate reached 98%.

Embodiment 2

On the basis of the preliminary test, firstly, farnesol and 10% difenoconazole microemulsion were prepared in proportion to a mother solution with a certain concentration gradient for use (farnesol: 0.5 μM, 10% difenoconazole microemulsion: dilution 5000 times). Pick a few basidiospores from the plate and culture them in 6 mL YEPS medium overnight as seed solution, transfer 500 μL to 50 mL YEPS medium for expansion the next day (to an OD ₆₀₀ of 0.2), and draw 500 μL of different concentrations of stock solutions respectively. Into a 5mL sterilized centrifuge tube, add an equal volume of bacterial solution and mix; then add 250μL of farnesol + 250μL to dilute 5000-fold 10% difenoconazole microemulsion into a 5mL sterilized centrifuge tube, add an equal volume of bacteria Mix 500 μL YEPS into a 5 mL sterilized centrifuge tube, add an equal volume of bacterial solution and mix well, place it at 28°C, and incubate in a shaker at 200 rpm/min for 24 h. Then, the bacterial liquid treated with each concentration was diluted 10 ³ times with fresh YEPS liquid medium, 200 μL was drawn and spread on YEPSA solid medium, and placed in a 28°C incubator for 48 hours of static culture. Finally, the virulence effects of farnesol and 10% difenoconazole microemulsion on the proliferation of black powdery mildew were determined.

The experimental results show (see Figure 2) that both difenoconazole and farnesol at a certain concentration in the figure have an inhibitory effect on the proliferation and growth of sugarcane black powder fungus. match, its virulence effect is more significant.

On the basis of the above, the concentration of farnesol is continuously reduced to seek the lowest effective concentration of farnesol. Control group used alone: farnesol: 0.4 μM, 10% difenoconazole microemulsion: 1×10 ⁵ times; mixed group (farnesol + 10% difenoconazole microemulsion): 0.1 μM+ 2×10 ⁵ times, 0.15 μM+2×10 ⁵ times, 0.2 μM+2×10 ⁵ times; control group: YEPS, treated as above. Incubate for 24h in a shaker at 200rpm/min at 28°C. Then, the bacterial liquid treated with each concentration was diluted 10 ⁴ times with fresh YEPS liquid medium, 200 μL was drawn and spread on YEPSA solid medium, and placed in a 28°C incubator for 48 hours of static culture. The number of colonies in each treatment was counted, and the synergistic ratio of each treatment group was calculated by taking the synergy of the control group as 1.

The experimental results show (see Figure 3), the effect of mixing farnesol and 10% difenoconazole microemulsion is more obvious than the effect of single use and double the concentration, indicating that the two have a synergistic effect. . ^The figure shows that with the increase of farnesol concentration, the synergistic effect continues to increase, and the optimal mixing ratio for the proliferation and virulence effect of S. Emulsion, the synergistic ratio reaches 74 times.

Embodiment 3

On the basis of the preliminary test, firstly, farnesol and 10% difenoconazole microemulsion were prepared in proportion to a mother solution with a certain concentration gradient for use (farnesol: 0.08 μM, 10% difenoconazole microemulsion: dilution 2 x 10 ⁴ times). Pick a few basidiospores from the plate and culture them in 6 mL YEPS medium overnight as seed solution, and transfer 500 μL to 50 mL YEPS medium for expansion (to an OD ₆₀₀ of 0.2) the next day. The concentration of farnesol was controlled to be 0.08 μM, the concentration of difenoconazole was diluted 2×10 ⁴ times, and then the gradient dilution was set to 0.5 times, 0.4 times, 0.25 times, 0.1 times, and 0.05 times of the mother liquor. Take the same 250 μL of farnesol and the same volume of different concentrations of difenoconazole and mix them in a centrifuge tube, and use 500 μL of farnesol and azoxystrobin stock solutions respectively as a single-use group; 500 μL of LYEPS is used as a blank control, and repeats are set. , add 500 μL of bacterial liquid to all centrifuge tubes and mix well, place in 28°C, 200rpm/min shaker for 20h, and then measure the final OD ₆₀₀ of each treatment, and calculate the net increase of OD ₆₀₀ to obtain the inhibition rate , the optimal compounding group for inhibiting black powdery mildew was obtained.

Referring to Figure 4, the experimental results show that Farnesol and Difenoconazole have a synergistic effect in the prevention and control of sugarcane smut, and the addition of farnesol can greatly reduce the dosage of chemical agents. When the concentration of farnesol is 0.04 μM, the lowest concentration of difenoconazole was 1×10 ⁵ times, and the inhibition rate was 0.91, which was higher than the effect of single use.

(As shown in the 7th group in Figure 4, it is 0.05Dif+0.5Far or 1×10 ⁵ times+0.04μM)

Embodiment 4

On the basis of the preliminary test, farnesol and 10% difenoconazole microemulsion were prepared into: farnesol 0.4 μM, 10% difenoconazole microemulsion diluted 1×10 ⁵ times mother solution for use. Pick a few basidiospores from the plate and culture them in 6 mL YEPS medium overnight as seed solution, transfer 500 μL to 50 mL YEPS medium the next day and expand the culture (to an OD ₆₀₀ of 0.2) for later use. Take 0.4 μM farnesol and difenoconazole stock solution diluted 1×10 ^{5 times, and 500 μL of YEPS respectively in different sterilized centrifuge tubes, and then take 0.4 μM farnesol and dilute 1×10 5 times} difenoconazole stock solution. 250 μL of each was placed in the same centrifuge tube, 500 μL of bacterial suspension was added to each centrifuge tube, and three replicates were set up. Incubate for 24h in a shaker at 200rpm/min at 28°C. Subsequently, the treated bacterial liquid in each centrifuge tube was diluted 10 ⁵ times with fresh YEPS liquid medium, 200 μL was drawn and spread on YEPSA solid medium, and placed in a 28° C. incubator for 48 hours of static culture. Count the number of colonies and observe the gain effect.

The results show (see Figure 5) that the mixing ratio of 0.2 μM farnesol + 10% difenoconazole microemulsion diluted 2 × 10 ⁵ times is also suitable for inhibiting the proliferation and growth of corn smut. It can be seen from the figure that 0.4 The inhibitory effect of μM farnesol and difenoconazole was obviously not as good as that achieved when the two were mixed and the concentration was reduced by half. When 0.2μM farnesol + dilute 2×10 ⁵ times 10% difenoconazole microemulsion, the virulence effect of stilbene zeae was higher than 95%.

Embodiment 5

On the basis of the preliminary test, firstly, farnesol and 30% hexaconazole suspending agent were prepared in proportion to a mother solution with a certain concentration gradient (farnesol: 0.2 μM, 0.3 μM, 0.5 μM; 30% hexaconazole suspending agent) (dilution): 2×10 ⁵ times,). Pick a few basidia spores from the plate and culture them in 6 mL YEPS medium overnight as seed solution, transfer 500 μL to 50 mL YEPS medium for expansion the next day (to OD600 of 0.2), and draw 500 μL of different concentrations of stock solution to In a 5mL sterilized centrifuge tube, add an equal volume of bacterial liquid and mix well, and record it as a single use group; in addition, draw 250 μL of different concentrations of farnesol stock solution and 250 μL of hexaconazole stock solution and mix them in a 5 mL sterilized centrifuge tube, add an equal volume of The bacterial liquid was mixed and recorded as the mixed use group; 500 μL of YEPS was pipetted into a 5 mL sterilized centrifuge tube, an equal volume of bacterial liquid was added and mixed, recorded as the control group, and cultured in a shaker at 28°C, 200 rpm/min for 24 hours. Then, the bacterial liquid treated with each concentration was diluted 103 times with fresh YEPS liquid medium, 200 μL was drawn and spread on YEPSA solid medium, and placed in a 28°C incubator for 48 hours of static culture. Finally, the virulence effects of farnesol and 30% hexaconazole suspension on the proliferation of S. cane were determined. (Image 6)

On the basis of the above, the concentration of farnesol is continuously reduced to seek the lowest effective concentration of farnesol. Control group alone: farnesol: 0.4 μM, 30% hexaconazole suspension: 2×10 ⁵ times; mixed group (farnesol+30% hexaconazole suspension): 0.1 μM+4×10 ⁵ times, 0.15 μM+4×10 ⁵ times, 0.2 μM+4×10 ⁵ times; control group: YEPS, treated as above. Incubate for 24h in a shaker at 200rpm/min at 28°C. Then, the bacterial liquid treated with each concentration was diluted 10 ⁴ times with fresh YEPS liquid medium, 200 μL was drawn and spread on YEPSA solid medium, and placed in a 28°C incubator for 48 hours of static culture. The number of colonies in each treatment was counted, and the synergistic ratio of each treatment group was calculated by taking the synergy of the control group as 1.

The experimental results show (see Figure 7) that the effect of mixing farnesol and 30% hexaconazole suspending agent is more obvious than that of single use and the concentration is doubled, indicating that the two have a synergistic effect. The figure shows that with the increase of farnesol concentration, the synergistic effect continues to increase, and the optimal mixing ratio for the proliferation and virulence of sugarcane black powder fungus is: 0.2μM + diluted 4×10 ⁵ times hexaconazole, the synergistic ratio reaches 62 times.

Embodiment 6

On the basis of the preliminary test, firstly, farnesol and 30% hexaconazole suspending agent were prepared in proportion to a mother solution with a certain concentration gradient (farnesol: 0.08 μM, 0.1 μM, 0.2 μM, 0.3 μM, 0.4 μM, 0.5 μM; 30% hexaconazole suspending agent (dilution): 2×10 ⁵ times,). Pick a few basidiospores from the plate and culture them in 6 mL YEPS medium overnight as seed solution. The next day, the cells are collected by centrifugation, and the cells are resuspended in YEPS liquid medium (OD ₆₀₀ is 0.2) for use. Pipette 300μL of different concentrations of stock solutions into 2mL sterilized centrifuge tubes respectively, and respectively pipette 150μL of farnesoid stock solution and 150μL of hexaconazole stock solution and mix them into 2mL sterile centrifuge tubes. 300YEPS is used as a control, and an equal amount of basidiospore suspension is added to mix. uniform, and three replicates were set for each concentration treatment. Then, after measuring the initial OD ₆₀₀ of each treatment with a spectrophotometer, it was placed in a shaker at 28°C and incubated at 200 rpm/min for 20 hours. After the final OD ₆₀₀ of each treatment was measured, the inhibition rate was calculated by calculating the net increase in OD ₆₀₀ . (Figure 8).

Referring to Figure 8, the effect of mixing farnesol and 30% hexaconazole suspending agent is obviously better than that of single use and the synergistic effect of doubling the concentration is even more obvious. 0.04 μM farnesol and hexaconazole diluted 4×10 ⁵ -fold achieved 94% inhibition, while farnesol diluted 2×10 ⁵ -fold exhibited 74% inhibition and 0.08 μM farnesol 8 %.

Claims (10)

1. The application of farnesol in the synergistic effect of triazole fungicides in the prevention and treatment of smut, wherein the triazole fungicides are difenoconazole and hexaconazole. 2. application according to claim 1, is characterized in that, described smut is the crop smut caused by sporozoite smut or smut. 3. application according to claim 2, is characterized in that, described smut is crop smut caused by sugarcane smut or corn smut. The application according to claim 1 or 2, characterized in that, the use concentration of the farnesol is 0.01 μM to 1 μM. 5. application according to claim 4 is characterized in that, difenoconazole is 10% microemulsion, and the use concentration is 5000～200000 times of dilution. 6. application according to claim 4 is characterized in that, hexaconazole is 30% microemulsion, and the use concentration is 100000～400000 times of dilution. 7. application according to claim 4, is characterized in that, to sugarcane black powder bacteria proliferation virulence effect, the mixing ratio of farnesol and difenoconazole is 0.15μM farnesol+diluted 2 × 10 ⁵ times 10% Difenoconazole Microemulsion. 8. application according to claim 4, it is characterised in that, to the virulence effect of corn smut proliferation, the mixing ratio of farnesol and difenoconazole is 0.2 μM farnesol+diluted 2×10 ⁵ times 10% Difenoconazole Microemulsion. 9. application according to claim 4, is characterized in that, to sugarcane black powdery mildew inhibitory effect, farnesol and difenoconazole mixing ratio is 0.04 μM farnesol+diluted 4 × 10 ⁵ times 10% Difenoconazole Microemulsion. 10. The application according to claim 4, characterized in that, for the proliferation and virulence effect of the sugarcane black powder fungus, the mixing ratio of farnesol and hexaconazole is 0.2 μM farnesol+dilution 4×10 ⁵ times 30% hexaconazole microemulsion.

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