CN108379603A - A kind of ultrasonic wave blood-plasma virus killing method and system - Google Patents
A kind of ultrasonic wave blood-plasma virus killing method and system Download PDFInfo
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- CN108379603A CN108379603A CN201810180160.7A CN201810180160A CN108379603A CN 108379603 A CN108379603 A CN 108379603A CN 201810180160 A CN201810180160 A CN 201810180160A CN 108379603 A CN108379603 A CN 108379603A
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- 210000002381 plasma Anatomy 0.000 title claims abstract description 104
- 241000700605 Viruses Species 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000002779 inactivation Effects 0.000 claims abstract description 48
- 239000011248 coating agent Substances 0.000 claims abstract description 10
- 238000000576 coating method Methods 0.000 claims abstract description 10
- 230000005284 excitation Effects 0.000 claims abstract description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000003546 nucleic acid damage Effects 0.000 claims abstract description 6
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 6
- 239000001301 oxygen Substances 0.000 claims abstract description 6
- 210000004369 blood Anatomy 0.000 claims description 31
- 239000008280 blood Substances 0.000 claims description 31
- 239000000463 material Substances 0.000 claims description 10
- 229920002472 Starch Polymers 0.000 claims description 3
- 230000000415 inactivating effect Effects 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 239000002574 poison Substances 0.000 claims 1
- 231100000614 poison Toxicity 0.000 claims 1
- 238000009738 saturating Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 238000010586 diagram Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000711549 Hepacivirus C Species 0.000 description 4
- 241000725303 Human immunodeficiency virus Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 238000001802 infusion Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000004779 membrane envelope Anatomy 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000002525 ultrasonication Methods 0.000 description 3
- 230000009385 viral infection Effects 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 241000598436 Human T-cell lymphotropic virus Species 0.000 description 2
- 241000710886 West Nile virus Species 0.000 description 2
- -1 albumen Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 208000006454 hepatitis Diseases 0.000 description 2
- 231100000283 hepatitis Toxicity 0.000 description 2
- 208000002672 hepatitis B Diseases 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 241000701076 Macacine alphaherpesvirus 1 Species 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 235000019628 coolness Nutrition 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004402 high myopia Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/21—Pharmaceuticals, e.g. medicaments, artificial body parts
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- External Artificial Organs (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The invention discloses a kind of ultrasonic wave blood-plasma virus killing method and system, method includes the following steps that S1. mixes blood plasma to be inactivated with methylenum careuleum；S2. emit ultrasonic wave to the blood plasma for being mixed with methylenum careuleum, make methylenum careuleum under the excitation of ultrasonic wave, generate active oxygen (ROS), keep the nucleic acid damage of virus, coating damaged, finally realize inactivation of virus；Its system includes inactivation plasma bags, and the inactivation container for accommodating the inactivation plasma bags, ultrasonic transducer from ultrasonic wave to the plasma bags and signal for emitting are connected to the ultrasonic power generator controller of the ultrasonic transducer；The method and system of the present invention can be uniformly to blood plasma carry out sound effect of irradiation, to greatly improve Plasma Inactivation quality and efficiency.
Description
Technical field
The present invention relates to blood-plasma virus killing field, specifically a kind of ultrasonic wave blood-plasma virus killing method and system.
Background technology
Blood transfusion is the indispensable important support condition of modern medicine, but objectively there is also relevant viruses of transfusing blood for blood transfusion
How infection risk by developing detection technique and Viral inactivation techniques constantly reduces correlated virus infection risk of transfusing blood, always
It is the problem of being concerned in blood transfusion medicine.Blood virus inactivation (virus inactinvation in blood
Products refer to) under the premise of keeping Blood Preparations validity, using the methods of physics, chemistry, biology, to that may deposit
It is that all viruses in Blood Preparations carry out inactivation treatment, to avoid High myopia therapy.Common inactivation of viruses method
Very much, but suitable for Blood Preparations the method being virus inactivated is seldom.Although the progress with blood testing technology and blood
Liquid management measure it is perfect, largely reduce blood transfusion caused by virus diffusibility, but due to virus become
It is different to cause immunoreactive change, immune silent infection and detection technique there are the limitation of window phase missing inspection, it examines in addition
The limitation of Pathogen category is surveyed, blood transfusion causes AIDS virus (HIV), Hepatitis C Virus (HCV), hepatitis type B virus
(HBV) risk cannot still prevent completely；Some uncommon viruses such as human t lymphotropic virus (HTLV), west
Nile virus (WNV), Epstein-Barr virus etc. most countries be not put into blood routine detection, these not after testing virus and
Other pathogenic microorganisms are also difficult to avoid completely by the risk of blood transfusion infection human body.Obviously, rely solely on blood donor screening and
Blood testing can not possibly prevent relevant virus infection of transfusing blood completely, therefore, can be by developing blood and blood constituent product disease
Malicious inactivation technology makes the relevant virus infection that fundamentally prevents to transfuse blood, ensures the reliable barrier of transfusion safety.And state
Border society is the same, the residual risk of China's infectious disease on blood transfusion also objective reality, and due in population of China hepatitis virus take
Band person's proportion is apparently higher than developed country, and the risk of Transfusion Transmission hepatitis virus also can accordingly increase.Blood-plasma virus killing
It is to study successful blood constituent Viral inactivation techniques at first, in current practice, mostly uses greatly based on photochemistry principle
Method.The innovation of the present invention is pioneering using ultrasonic wave progress blood-plasma virus killing.
Existing research fully proves at present, and methylenum careuleum (methylene blue, hereinafter referred MB) can be with the core of virus
Acid is combined with lipid envelope, under the action of visible light, can make the nucleic acid break of virus, coating is damaged, thus can kill packet
Include the lipid envelope virus and part non-lipid coating of AIDS virus (HIV), hepatitis B (HBV), hepatitis C virus (HCV) etc.
Virus.Therefore, in the prior art, the photosensitive method of generally use methylenum careuleum carries out the inactivation of virus of blood plasma, however, to be mixed with albumen,
The substances such as fat, the poor blood plasma of transparency is more apparent to the blocking of light, so cause to the light action inside plasma bags compared with
It is weak, when being virus inactivated using the photosensitive method of methylenum careuleum, cannot light irradiation equably be carried out to blood plasma in plasma bags, cause to inactivate
When, the light irradiation that part blood plasma is subject to is insufficient, influences the effect of blood-plasma virus killing, and action time is longer, generally 35 points
Clock or so.
Existing research, which demonstrates ultrasonic wave, can also activate MB to generate active oxygen (ROS), and type is mainly singlet oxygen
(1O2) and hydroxy radical (OH), the energy of ROS can destroy the nucleic acid of virus, especially guanine, lead to the broken of viral gene
It is bad, fractionated viral coating can also be destroyed, achievees the effect that inactivation of virus.
Invention content
In view of this, carrying out blood-plasma virus killing using ultrasonic wave the purpose of the present invention is pioneering, existing illumination is overcome to go out
The defects of live virus technology, providing one kind can go out uniformly to blood plasma carry out sound effect of irradiation to greatly improve blood plasma
The ultrasonic wave blood-plasma virus killing method and system of bioplasm amount and efficiency.
The ultrasonic wave blood-plasma virus killing method of the present invention, includes the following steps：S1. by blood plasma and methylenum careuleum to be inactivated
Mixing；S2. emit ultrasonic wave to the blood plasma for being mixed with methylenum careuleum, make methylenum careuleum under the excitation of ultrasonic wave, generate active oxygen ROS,
Keep nucleic acid damage, the coating of virus damaged, it is final to realize inactivation；
Further, in step s 2, blood plasma to be inactivated is placed in plasma bags, using flat ultrasonic transducer to
Plasma bags emit ultrasonic wave；
Further, in step s 2, using multiple flat ultrasonic transducers respectively towards different directions to the blood plasma
Bag transmitting ultrasonic wave；
Further, in step s 2, the conduit made using acoustic window material conveys blood plasma to be inactivated；Using burnt formula ultrasonic wave
Focusing ultrasonic wave in the conduit, is made the blood plasma conveyed in conduit can be by ul-trasonic irradiation region by energy converter；
It is described for accommodating the invention also discloses a kind of ultrasonic wave blood-plasma virus killing system, including inactivation plasma bags
Inactivate plasma bags inactivation container, for the plasma bags emit ultrasonic wave ultrasonic transducer and signal be connected to institute
State the ultrasonic power generator controller of ultrasonic transducer；
Further, the inactivation container is cube structure；The ultrasonic transducer is flat ultrasonic transducer,
The flat ultrasonic transducer is side and bottom surface that be multiple and being separately positioned on inactivation container；
The invention also discloses another ultrasonic wave blood-plasma virus killing system, include for convey blood plasma to be inactivated,
The conduit made by acoustic window material, for by focusing ultrasonic wave in the conduit, make the blood plasma conveyed in conduit can be by super
The focusing ultrasonic transducer and signal in sound wave effect region are connected to the ultrasonic wave of the focusing ultrasonic transducer
Power generator controller；
Further, the focusing ultrasonic transducer is concave disc type ultrasonic transducer, and focus point is located at described lead
In pipe；
Further, the focusing ultrasonic transducer is drum type brake ultrasonic transducer；The conduit is passed through along axis
The drum type brake ultrasonic transducer；
Further, the focusing ultrasonic transducer is the circular arc type ultrasonic transducer of multi-disc enclosing said catheter.
The beneficial effects of the invention are as follows：
The ultrasonic wave blood-plasma virus killing method and system of the present invention, over-extraction are mixed with methylenum careuleum with ultrasonic irradiation and wait going out
Promoting blood circulation slurry, makes methylenum careuleum under the excitation of ultrasonic wave, generates singlet oxygen, keeps the nucleic acid break of virus, coating damaged, final real
It now inactivating, is virus inactivated compared to the photosensitive method of existing methylenum careuleum, penetration capacity of the ultrasonic wave in liquid (blood plasma) is stronger,
Especially to the blood plasma for the color that has powerful connections (normal plasma is faint yellow), especially to being mixed with the substances such as albumen, fat, transparency
Poor blood plasma, it is more apparent to the blocking of light, and then cause it is weaker to the light action inside plasma bags, cannot be equably to blood
It starches blood plasma in bag and carries out light irradiation, and ultrasonic wave is good propagated in liquid, can ensure that the uniform acoustically radiating to blood plasma in bag
According to effect.
Description of the drawings
The invention will be further described with reference to the accompanying drawings and examples：
Fig. 1 is the structural schematic diagram of first embodiment of the invention；
Fig. 2 is the structural schematic diagram of the inactivation case of second embodiment of the invention；
Fig. 3 is the structural schematic diagram of third embodiment of the invention；
Fig. 4 is the structural schematic diagram of fourth embodiment of the invention；
Fig. 5 is the sectional view of fourth embodiment of the invention；
Fig. 6 is the sectional view of fifth embodiment of the invention.
Specific implementation mode
First embodiment
Fig. 1 is the structural schematic diagram of first embodiment.As shown, the ultrasonic wave blood-plasma virus killing system in the present embodiment
System, including inactivation plasma bags 11, the inactivation container 12 for accommodating the inactivation plasma bags 11, for being sent out to the plasma bags 11
It penetrates the ultrasonic transducer 14 of ultrasonic wave and ultrasonic power that signal is connected to the ultrasonic transducer 14 controls
Device 16；When Plasma Inactivation, inactivation 12 planted agent of container fills couplant 13 [such as degassing pure water], is conducted to blood plasma conducive to ultrasonic wave
Bag 11, ultrasonic transducer 14 is flat ultrasonic transducer 14 in the present embodiment, and concrete structure is the prior art, herein
It does not repeat, function is that the electric energy of input is converted into mechanical energy (i.e. ultrasonic wave) to pass again, the flat ultrasonic waves
Energy device 14 is mounted on the bottom surface of inactivation container 12 of cube structure.Blood plasma to be inactivated in plasma bags 11 is inactivated
When, controlled electric energy is sent to ultrasonic transducer 14, ultrasonic wave transducer by ultrasonic power generator controller 16 by conducting wire 15
Device 14 converts electrical energy into ultrasonic wave and emits upwards, and the plasma bags 11 being placed in inactivation container 12 are received by couplant
13 ultrasonic waves propagated.Plasma bags 11 include the blood plasma for being virus inactivated processing and the MB being pre-mixed, MB in need with
Virus generates ROS under the excitation of ultrasonic wave, makes the nucleic acid damage of virus, and coating is damaged, thus can kill lipid envelope virus
With part non-lipid enveloped virus.After being ultrasonically treated, MB and sound solution are filtered to remove with the blood transfusion filter with adsorbing medium
Product, stored frozen are used for clinical infusion.
Second embodiment
Fig. 2 is the structural schematic diagram of second embodiment of the invention, compares first embodiment, flat in second embodiment
Ultrasonic transducer 21 is three, is separately positioned on the bottom surface and two adjacent sides of inactivation container of cube structure, this
Sample can enhance the ul-trasonic irradiation face of inactivation blood bag, enhance ultrasonic intensity, improve ultrasonication effect, reduce ul-trasonic irradiation
Time.In addition, the ultrasonic transducer and ultrasonic power generator controller in the present embodiment are the prior art, such as can adopt
Energy converter in supersonic generator disclosed in Chinese invention patent with Publication No. 101658838A and power control electricity
Road can not only be acted in the power supply of ultrasonic transducer 21, can also adjust energy size to adapt to different ultrasounds
Strength demand can monitor actual ultrasound intensity with feedback.
Using in the present embodiment inactivation system carry out blood-plasma virus killing the step of be：S1. by blood plasma to be inactivated with
Methylenum careuleum mixes；S2. emit ultrasonic wave to the blood plasma for being mixed with methylenum careuleum, make methylenum careuleum under the excitation of ultrasonic wave, generate activity
Oxygen keeps the nucleic acid damage of virus, coating damaged, final to realize inactivation；In step s 2, using multiple flat ultrasonic wave transducers
Device emits ultrasonic wave towards different directions to the plasma bags respectively.
3rd embodiment
Fig. 3 is the structural schematic diagram of the third embodiment of the present invention, the ultrasonic wave blood-plasma virus killing system of the present embodiment
Include for convey blood plasma to be inactivated, by sound translative performance compared with the conduit 31 that good material makes, for by focusing ultrasonic wave in institute
It states conduit 31, the blood plasma conveyed in conduit 31 is made to focus ultrasonic wave transducer by the concave disc type of the focusing ultrasonic wave zone of action
Device 34 and signal are connected to the ultrasonic power generator controller 32 of the focusing ultrasonic transducer 34, and blood plasma carries out
When inactivation of virus, the controlled electric energy that control unit generates is occurred by ultrasonic power, ultrasonic wave transducer is transferred to by conducting wire 33
Device 34, ultrasonic transducer 34 convert electrical energy into ultrasonic wave because concave disc type focusing ultrasonic energy converter 34 be recessed plate-like (such as
It is the same with solar cooker) it can be converged in a smaller zone of action by ultrasonic wave in couplant, thus can get energy
Higher ultrasonication intensity, needs the blood plasma being virus inactivated to be pre-mixed MB, the flowing in conduit 31, and passes through the work
When with region, it is virus inactivated.
Conduit 31 in the present embodiment is that the preferable inert material pipeline of sound transparency is made that [sound translative performance refers to compared with good material
The Characteristic impedance of Characteristic impedance and water matches, and to sound energy loss very little, can make the incident sound wave overwhelming majority in water
The material of transmission, for example, polytetrafluoroethylene (PTFE) (e-PTFE) make thin-wall tube, sound translative performance is preferable, e-PTFE surface inertness pole
By force, so stability is splendid, biologically there is safety guarantee for Plasma Inactivation.And it is disposable, it is ensured that blood plasma is exempted from
By accidental pollution；And when inactivation of virus, ultrasonic wave blood-plasma virus killing connection bag can be used.Inactivation connection bag includes former bag 35, inactivation
The both ends of back pkt. 36, conduit 31 and folding siphunculus 37, conduit 31 pass through a folding siphunculus 37 and the original tape and inactivation back pkt. respectively
36 oral area connection.The blood plasma of non-inactivation of viruses in former bag 35, when inactivation system through this embodiment is virus inactivated,
First fracture siphunculus 37, and unicom original bag 35 and inactivation back pkt. 36, blood plasma are virus inactivated when flowing through conduit 31.By ultrasonic wave
After processing, it is filtered to remove MB and sound solution product with the blood transfusion filter with adsorbing medium, stored frozen is used for clinical infusion.
Fourth embodiment
Fig. 4 is the structural schematic diagram of the present embodiment；Fig. 5 is the sectional view of the present embodiment, as shown, the present embodiment is super
Sound wave blood-plasma virus killing system includes for conduit 41 conveying blood plasma to be inactivated, being made by acoustic window material, and drum type brake is poly-
Burnt ultrasonic transducer 42 and signal are connected to the ultrasonic power generator controller of the focusing ultrasonic transducer 42
44, conduit 41 coaxially passes through drum type brake focusing ultrasonic energy converter 42, when blood plasma is virus inactivated, is occurred by ultrasonic power
The controlled electric energy that controller 44 generates is transferred to drum type brake focusing ultrasonic energy converter 42 by conducting wire 43, and drum type brake focuses super
Acoustic wave transducer 42 converts electrical energy into ultrasonic wave, and because the ultrasonic wave surface of emission of drum type brake focusing ultrasonic energy converter 42 is
Ultrasonic wave can be converged the stronger region (linearity region to form straight line shape by the inner circle of cylinder in the axial location of inner circle
Overlapped with the circulating area of conduit 41 or the circulating area of conduit 41 include the linearity region), thus it is higher to can get energy
Ultrasonication intensity, to improve the quality and efficiency of ultrasonic wave inactivation of virus.The blood plasma being virus inactivated is needed to be pre-mixed
MB is flowed in conduit 41 and is virus inactivated.After being ultrasonically treated, it is filtered to remove with the blood transfusion filter with adsorbing medium
MB and sound solution product, stored frozen are used for clinical infusion.
5th embodiment
In the present embodiment, the drum type brake focusing ultrasonic energy converter 51 in fourth embodiment is replaced with described in two panels encirclement
The circular arc type ultrasonic transducer 51 of conduit 52 (also includes certainly, ultrasonic power generator controller 54 and leading in the present embodiment
Line 53), the circular arc type ultrasonic transducer 51 used in the present embodiment for semi arch structure (i.e. semicircular cylinder), appoint by arc radius
Meaning, but according to focusing principle, no matter arc radius is how many, and finally formed focusing supersonic region is linear.This reality
This split structure of example is applied, convenient for placing inactivation dedicated conduits 52 therein.In the present embodiment, the semi-circular ultrasonic wave of two panels
The distance between energy converter 51 is adjustable, separates two panels energy converter 51 when being put into or taking out conduit 52, is conveniently operated, and is carrying out
Ultrasonic wave is closed when inactivating, it is ensured that ul-trasonic irradiation effect.In addition, since ultrasonic transducer 51 is converting electrical energy into
It can generate heat when acoustic energy, to ensure that the normal work of system, the ultrasonic transducer 51 in each embodiment of the present invention can be used existing
Some water coolings or air-cooled cooling system are cooled down.After being ultrasonically treated, filtered with the blood transfusion filter with adsorbing medium
MB and sound solution product are removed, stored frozen is used for clinical infusion.Using the inactivation system in third to five embodiments into promoting circulation of blood
Starch inactivation of virus the step of be：S1. blood plasma to be inactivated is mixed with methylenum careuleum；S2. emit to the blood plasma for being mixed with methylenum careuleum super
Sound wave makes methylenum careuleum under the excitation of ultrasonic wave, generates active oxygen, keeps the nucleic acid damage of virus, coating damaged, and final realize is gone out
It is living.In step s 2, the conduit made using acoustic window material conveys blood plasma to be inactivated；It will be surpassed using focusing ultrasonic transducer
Sound wave focuses on the conduit, makes the blood plasma conveyed in conduit can be by ul-trasonic irradiation region.
Finally illustrate, the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although with reference to compared with
Good embodiment describes the invention in detail, it will be understood by those of ordinary skill in the art that, it can be to the skill of the present invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the right of invention.
Claims (10)
1. a kind of ultrasonic wave blood-plasma virus killing method, which is characterized in that include the following steps：
S1. blood plasma to be inactivated is mixed with methylenum careuleum；
S2. emit ultrasonic wave to the blood plasma for being mixed with methylenum careuleum, make methylenum careuleum under the excitation of ultrasonic wave, generate active oxygen, make disease
Nucleic acid damage, the coating of poison are damaged, final to realize inactivation.
2. ultrasonic wave blood-plasma virus killing method according to claim 1, it is characterised in that：In step s 2, it will wait going out
Blood plasma living is placed in plasma bags, emits ultrasonic wave to plasma bags using flat ultrasonic transducer.
3. ultrasonic wave blood-plasma virus killing method according to claim 2, it is characterised in that：In step s 2, using more
A flat ultrasonic transducer emits ultrasonic wave towards different directions to the plasma bags respectively.
4. ultrasonic wave blood-plasma virus killing method according to claim 1, it is characterised in that：In step s 2, using saturating
The conduit that sound material makes conveys blood plasma to be inactivated；Using focusing ultrasonic transducer by focusing ultrasonic wave in the conduit,
Make the blood plasma conveyed in conduit can be by ul-trasonic irradiation region.
5. a kind of ultrasonic wave blood-plasma virus killing system, it is characterised in that：Including inactivating plasma bags, for accommodating the inactivation blood
Starch bag inactivation container, for the plasma bags emit ultrasonic wave ultrasonic transducer and signal be connected to the ultrasound
The ultrasonic power generator controller of wave transducer.
6. ultrasonic wave blood-plasma virus killing system according to claim 5, it is characterised in that：The inactivation container is cube
Body structure；The ultrasonic transducer is flat ultrasonic transducer, which is multiple and difference
It is arranged in the side and bottom surface of inactivation container.
7. a kind of ultrasonic wave blood-plasma virus killing system, it is characterised in that：Include for convey blood plasma to be inactivated, by entrant sound material
Expect make conduit, for by focusing ultrasonic wave in the conduit, make the blood plasma conveyed in conduit that can pass through ul-trasonic irradiation
The ultrasonic power that the focusing ultrasonic transducer and signal in region are connected to the focusing ultrasonic transducer occurs
Controller.
8. ultrasonic wave blood-plasma virus killing system according to claim 7, it is characterised in that：The focusing ultrasonic waves
Energy device is concave disc type ultrasonic transducer, and focus point is located in the conduit.
9. ultrasonic wave blood-plasma virus killing system according to claim 7, it is characterised in that：The focusing ultrasonic waves
Energy device is drum type brake ultrasonic transducer；The conduit passes through the drum type brake ultrasonic transducer along axis.
10. ultrasonic wave blood-plasma virus killing system according to claim 7, it is characterised in that：The focusing ultrasonic wave
Energy converter is the circular arc type ultrasonic transducer of multi-disc enclosing said catheter.
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| CN201810180160.7A CN108379603A (en) | 2018-03-05 | 2018-03-05 | A kind of ultrasonic wave blood-plasma virus killing method and system |
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| CN201810180160.7A CN108379603A (en) | 2018-03-05 | 2018-03-05 | A kind of ultrasonic wave blood-plasma virus killing method and system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113181547A (en) * | 2021-04-23 | 2021-07-30 | 清华大学 | Heart pump with ultrasonic thrombolysis function |
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|---|---|---|---|---|
| CN88102062A (en) * | 1987-04-07 | 1988-10-26 | B·B·C·股份有限公司 | The method and apparatus of disinfecting utensils |
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| CN113181547B (en) * | 2021-04-23 | 2022-03-18 | 清华大学 | Heart pump with ultrasound thrombolysis |
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