CN108358322A - A kind of strain formula and preparation method thereof for administering black and odorous water - Google Patents
A kind of strain formula and preparation method thereof for administering black and odorous water Download PDFInfo
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- CN108358322A CN108358322A CN201810121100.8A CN201810121100A CN108358322A CN 108358322 A CN108358322 A CN 108358322A CN 201810121100 A CN201810121100 A CN 201810121100A CN 108358322 A CN108358322 A CN 108358322A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000009472 formulation Methods 0.000 title description 4
- 241000894006 Bacteria Species 0.000 claims abstract description 85
- 241000186046 Actinomyces Species 0.000 claims abstract description 39
- 244000005700 microbiome Species 0.000 claims abstract description 39
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 36
- 108090000790 Enzymes Proteins 0.000 claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 24
- 241000605154 Nitrobacter winogradskyi Species 0.000 claims abstract description 23
- 241000589597 Paracoccus denitrificans Species 0.000 claims abstract description 23
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 23
- 241001478240 Coccus Species 0.000 claims abstract description 22
- 241001147790 [Clostridium] papyrosolvens Species 0.000 claims abstract description 20
- 230000009935 nitrosation Effects 0.000 claims abstract description 18
- 238000007034 nitrosation reaction Methods 0.000 claims abstract description 18
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 14
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 14
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 11
- 241000589565 Flavobacterium Species 0.000 claims abstract description 11
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 11
- 241000605118 Thiobacillus Species 0.000 claims abstract description 11
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000607598 Vibrio Species 0.000 claims abstract description 10
- 206010047400 Vibrio infections Diseases 0.000 claims abstract description 10
- 235000019157 thiamine Nutrition 0.000 claims abstract description 10
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims abstract description 7
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229960003495 thiamine Drugs 0.000 claims abstract description 7
- 239000011721 thiamine Substances 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 7
- 244000025254 Cannabis sativa Species 0.000 claims description 6
- 241000589516 Pseudomonas Species 0.000 claims description 5
- 108090000787 Subtilisin Proteins 0.000 claims description 5
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 238000000855 fermentation Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 239000005864 Sulphur Substances 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 8
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 238000011065 in-situ storage Methods 0.000 abstract description 3
- 238000004065 wastewater treatment Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 6
- 102000004882 Lipase Human genes 0.000 description 4
- 108090001060 Lipase Proteins 0.000 description 4
- 239000004367 Lipase Substances 0.000 description 4
- 241000194017 Streptococcus Species 0.000 description 4
- 235000019421 lipase Nutrition 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 150000003544 thiamines Chemical class 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000011953 bioanalysis Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 230000000855 fungicidal effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- 239000008239 natural water Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 2
- 102000010637 Aquaporins Human genes 0.000 description 1
- 108010063290 Aquaporins Proteins 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 241001609931 bacterium 20 Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000037081 physical activity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- CADICXFYUNYKGD-UHFFFAOYSA-N sulfanylidenemanganese Chemical compound [Mn]=S CADICXFYUNYKGD-UHFFFAOYSA-N 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/26—H2S
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The present invention discloses a kind of strain formula for administering black and odorous water, it is characterised in that：It includes following strains：Photosynthetic bacteria, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism, the complex microorganism include pseudomonad, vibrios, Flavobacterium, the pure coccus of nitrous acid, thiobacillus thiooxidant, bacillus subtilis, bacillus licheniformis solves thiamine bud pole bacterium, the bacterium number of saccharomycete, denitrogenation coccus, actinomyces.Also disclose the preparation method for the strain formula for administering black and odorous water.When the present invention is for creek, lake, river stream wastewater treatment, which can effectively excite the activity for the microorganism having in water body in itself, accelerate the growth and breeding of microorganism, and good supporting function is provided for in-situ immobilization.
Description
Technical field
The present invention relates to field of agricultural microbial technology, and in particular to a kind of strain formula and its system for administering black and odorous water
Preparation Method.
Background technology
The urban water-bodies such as river are the important components of urban ecological system, and, water body small with environmental capacity of water is certainly
Net energy force difference, it is easy to pollute the features such as, and frequently as the important blowdown in city incity, receive dirty channel, water quality is easier to deteriorate.It is a large amount of dirty
The inflow of dye object causes urban water-body eutrophication serious, causes the drastically consumption of Dissolved Oxygen in Water, leads to water body long term hypoxia
To which black smelly state be presented.The appearance of the urban water-bodies such as river is black smelly, oneself becomes the universal phenomenon in China city, seriously affects bank
Resident living and city image.
The urban water-bodies such as river it is black it is smelly mainly receive dirt due to excessive and cause water ecology unbalance, water hypoxia or even anaerobism
Under the conditions of pollutant convert and generate the black such as the odorants such as ammonia nitrogen, hydrogen sulfide, volatile organic acids and iron, manganese sulfide
Substance.
Currently, the administering method of city black and odorous water has Physical, chemical method and bioanalysis.Wherein, in bioanalysis usually
Microbe preparation administers city black and odorous water, and a certain amount of microorganism formulation is added into water body, accelerates to pollute in water body
Object is degraded, and the self-purification function of water body is enhanced.But microorganism formulation is usually present pair in the application process for administering black and odorous water
The degradation capabilities of the pollutants such as nitrogen, phosphorus and organic matter is weak, treatment effeciency is low, the elimination to odorous gas hydrogen sulfide in black and odorous water
The problems such as acting on unobvious.
Invention content
In order to solve the above technical problem, the present invention provides a kind of strain formula for administering black and odorous water and its preparation sides
Method, photosynthetic bacteria provided by the invention, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme, molten paper shuttle
Bacterium, actinomyces, Paracoccus denitrificans and complex microorganism are obtained by the microculture domestication extracted in natural water, are passed through
Have the characteristics that species diversity, activity is high, efficiency is fast, resistance is strong after combination；The microbial bacteria agent prescription improves contaminant water
The with obvious effects of body, the microculture period is short, strain diversity is good, and in the complex micro organism fungicide comprising a variety of anaerobism,
Aerobic, facultative anaerobic bacteria can effectively remove protide, cellulose, carbohydrate, pathogen, virus, sulfur-bearing object in sewage
Matter, and can effectively adsorb heavy metal, efficiently removes COD, and ammonia nitrogen while also has the function of sterilizing；For river
Gush, lake, river stream wastewater treatment when, which can effectively excite the microorganism having in itself in water body
Activity, accelerates the growth and breeding of microorganism, and good supporting function is provided for in-situ immobilization.The present invention overcomes existing skills
The deficiency of art provides a kind of strain formula for administering black and odorous water, with obvious effects to purifying water body, improvement water body environment.
The present invention is achieved through the following technical solutions, and the present invention provides a kind of strain formula for administering black and odorous water,
It includes following strains：Photosynthetic bacteria, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme, molten paper shuttle
Bacterium, actinomyces, Paracoccus denitrificans and complex microorganism, the complex microorganism include pseudomonad, vibrios, Flavobacterium, nitrous
The pure coccus of acid, thiobacillus thiooxidant, bacillus subtilis, bacillus licheniformis, solution thiamine bud pole bacterium, the bacterium number of saccharomycete,
Denitrogenation coccus, actinomyces.
Preferably, the complex microorganism includes the raw material composition of following parts by weight：10~20 parts of pseudomonad, arc
10~15 parts of bacterium, 10~15 parts of Flavobacterium, 10~15 parts of the pure coccus of nitrous acid, 10~15 parts of thiobacillus thiooxidant, withered grass bud pole
10~20 parts of bacterium, 5~15 parts of bacillus licheniformis, 5~10 parts of solution thiamine bud pole bacterium, 5~10 parts of saccharomycete, denitrogenation coccus
5~10 parts, 5~10 parts of actinomyces.
Preferably, the group that the strain includes following parts by weight is grouped as：5~12 parts of photosynthetic bacteria, bud pole bacterium
20~30 parts, 5~10 parts of Clostridium papyrosolvens, 5~10 parts, 8~10 parts Paracoccus denitrificans of actinomyces, 10~20 parts of nitrosation bacillus,
10~20 parts of bacterium nitrobacters, 10~20 parts of denitrification bacillus and 1~5 part of biological enzyme, 5~10 parts of Clostridium papyrosolvens, actinomyces 8~10
Part, 20~30 parts of 1~5 part of Paracoccus denitrificans and complex microorganism.
Preferably, the bud pole bacterium uses bacillus subtilis and person's lichens bud pole bacterium.
Preferably, the bacterium colony ratio of the withered grass bud bubble bacillus and the lichens bud pole bacterium is 1：1-2：8.
Preferably, the actinomyces include streptococcus.
Preferably, the photosynthetic bacteria includes bath pool red pseudomonas.
Preferably, the biological enzyme includes lipase and subtilisin.
A kind of preparation method of strain formula that administering black and odorous water, includes the following steps：
S1：Configure culture medium：
S2：Inoculation：Photosynthetic bacteria, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, anti-is added in the culture medium prepared into S1
After bacterium nitrobacter, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism co-fermentation, filter is obtained by filtration
Liquid；
S3：With the filtrate prepared in protection liquid dilution S2.
Beneficial effects of the present invention are：
1) photosynthetic bacteria provided by the invention, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme are molten
Paper clostridium, actinomyces, Paracoccus denitrificans and complex microorganism are to be tamed by the microculture extracted in natural water and obtained
It arrives, has the characteristics that species diversity, activity is high, efficiency is fast, resistance is strong after combined；
2) the microbial bacteria agent prescription improve the with obvious effects of polluted-water, the microculture period is short, strain diversity is good, and
Comprising a variety of anaerobism, aerobic, facultative anaerobic bacteria in the complex micro organism fungicide, can effectively remove protide in sewage,
Cellulose, carbohydrate, pathogen, virus, sulphur-containing substance, and heavy metal can be effectively adsorbed, COD is efficiently removed, ammonia nitrogen
Also has the function of sterilizing simultaneously；
3) when being used for creek, lake, river stream wastewater treatment, which can effectively excite in water body deposits in itself
The activity of some microorganisms, accelerates the growth and breeding of microorganism, and good supporting function is provided for in-situ immobilization.
4）The present invention overcomes the deficiencies in the prior art, a kind of strain formula for administering black and odorous water are provided, to purification
Water body, improvement water body environment are with obvious effects.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
With reference to and specific embodiment to the present invention application principle be further described.
Embodiment 1：A kind of strain formula for administering black and odorous water comprising following strains：Photosynthetic bacteria, bud pole bacterium,
Nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism,
The complex microorganism includes pseudomonad, vibrios, Flavobacterium, the pure coccus of nitrous acid, thiobacillus thiooxidant, bacillus subtilis,
Bacillus licheniformis solves thiamine bud pole bacterium, the bacterium number of saccharomycete, denitrogenation coccus, actinomyces.
The complex microorganism includes the raw material composition of following parts by weight：10 parts of pseudomonad, 10 parts of vibrios, Flavobacterium
10 parts, 10 parts of the pure coccus of nitrous acid, 10 parts of thiobacillus thiooxidant, 10 parts of bacillus subtilis, 5 parts of bacillus licheniformis, solution thiamines
5 parts of plain bud pole bacterium, 5 parts of saccharomycete, 5 parts of denitrogenation coccus, 5 parts of actinomyces.
The strain includes that the group of following parts by weight is grouped as：5 parts of photosynthetic bacteria, 20 parts of bud pole bacterium, Clostridium papyrosolvens 5
Part, 5 parts, 8 parts Paracoccus denitrificans of actinomyces, 10 parts of nitrosation bacillus, 10 parts of bacterium nitrobacters, 10 parts of denitrification bacillus and 1 part of life
20 parts of object enzyme, 5 parts of Clostridium papyrosolvens, 8 parts of actinomyces, 1 part of Paracoccus denitrificans and complex microorganism.
The bud pole bacterium uses bacillus subtilis and person's lichens bud pole bacterium.
The bacterium colony ratio of the withered grass bud bubble bacillus and the lichens bud pole bacterium is 1：2.
The actinomyces include streptococcus.
The photosynthetic bacteria includes bath pool red pseudomonas.
The biological enzyme includes lipase and subtilisin.
A kind of preparation method of strain formula that administering black and odorous water, includes the following steps：
S1：Configure culture medium：
S2：Inoculation：Photosynthetic bacteria, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, anti-is added in the culture medium prepared into S1
After bacterium nitrobacter, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism co-fermentation, filter is obtained by filtration
Liquid；
S3：With the filtrate prepared in protection liquid dilution S2.
Embodiment 2：A kind of strain formula for administering black and odorous water comprising following strains：Photosynthetic bacteria, bud pole bacterium,
Nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism,
The complex microorganism includes pseudomonad, vibrios, Flavobacterium, the pure coccus of nitrous acid, thiobacillus thiooxidant, bacillus subtilis,
Bacillus licheniformis solves thiamine bud pole bacterium, the bacterium number of saccharomycete, denitrogenation coccus, actinomyces.
The complex microorganism includes the raw material composition of following parts by weight：20 parts of pseudomonad, 15 parts of vibrios, Flavobacterium
15 parts, 15 parts of the pure coccus of nitrous acid, 15 parts of thiobacillus thiooxidant, 20 parts of bacillus subtilis, 15 parts of bacillus licheniformis, solution thiamines
10 parts of plain bud pole bacterium, 10 parts of saccharomycete, 10 parts of denitrogenation coccus, 10 parts of actinomyces.
The strain includes that the group of following parts by weight is grouped as：12 parts of photosynthetic bacteria, 30 parts of bud pole bacterium, Clostridium papyrosolvens
10 parts, 10 parts, 10 parts Paracoccus denitrificans of actinomyces, 20 parts of nitrosation bacillus, 20 parts of bacterium nitrobacters, 120 parts of denitrification bacillus and 5
30 parts of part biological enzyme, 10 parts of Clostridium papyrosolvens, 10 parts of actinomyces, 5 parts of Paracoccus denitrificans and complex microorganism.
The bud pole bacterium uses bacillus subtilis and person's lichens bud pole bacterium.
The bacterium colony ratio of the withered grass bud bubble bacillus and the lichens bud pole bacterium is 1：8.
The actinomyces include streptococcus.
The photosynthetic bacteria includes bath pool red pseudomonas.
The biological enzyme includes lipase and subtilisin.
A kind of preparation method of strain formula that administering black and odorous water, includes the following steps：
S1：Configure culture medium：
S2：Inoculation：Photosynthetic bacteria, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, anti-is added in the culture medium prepared into S1
After bacterium nitrobacter, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism co-fermentation, filter is obtained by filtration
Liquid；
S3：With the filtrate prepared in protection liquid dilution S2.
Embodiment 3：A kind of strain formula for administering black and odorous water comprising following strains：Photosynthetic bacteria, bud pole bacterium,
Nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism,
The complex microorganism includes pseudomonad, vibrios, Flavobacterium, the pure coccus of nitrous acid, thiobacillus thiooxidant, bacillus subtilis,
Bacillus licheniformis solves thiamine bud pole bacterium, the bacterium number of saccharomycete, denitrogenation coccus, actinomyces.
The complex microorganism includes the raw material composition of following parts by weight：15 parts of pseudomonad, 12 parts of vibrios, Flavobacterium
12 parts, 12 parts of the pure coccus of nitrous acid, 12 parts of thiobacillus thiooxidant, 15 parts of bacillus subtilis, 10 parts of bacillus licheniformis, solution thiamines
8 parts of plain bud pole bacterium, 8 parts of saccharomycete, 8 parts of denitrogenation coccus, 8 parts of actinomyces.
The strain includes that the group of following parts by weight is grouped as：8 parts of photosynthetic bacteria, 25 parts of bud pole bacterium, Clostridium papyrosolvens 8
Part, 8 parts, 9 parts Paracoccus denitrificans of actinomyces, 15 parts of nitrosation bacillus, 15 parts of bacterium nitrobacters, 15 parts of denitrification bacillus and 3 parts of lifes
25 parts of object enzyme, 8 parts of Clostridium papyrosolvens, 9 parts of actinomyces, 3 parts of Paracoccus denitrificans and complex microorganism.
The bud pole bacterium uses bacillus subtilis and person's lichens bud pole bacterium.
The bacterium colony ratio of the withered grass bud bubble bacillus and the lichens bud pole bacterium is 1：4.
The actinomyces include streptococcus.
The photosynthetic bacteria includes bath pool red pseudomonas.
The biological enzyme includes lipase and subtilisin.
A kind of preparation method of strain formula that administering black and odorous water, includes the following steps：
S1：Configure culture medium：
S2：Inoculation：Photosynthetic bacteria, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, anti-is added in the culture medium prepared into S1
After bacterium nitrobacter, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism co-fermentation, filter is obtained by filtration
Liquid；
S3：With the filtrate prepared in protection liquid dilution S2.
Certainly, above description is also not limited to the example above, technical characteristic of the present invention without description can by or
It is realized using the prior art, details are not described herein；It is not to this that above example, which is only used to illustrate the technical scheme of the present invention,
The limitation of invention, describes the invention in detail with reference to preferred embodiment, and those skilled in the art should
Understand, the variations, modifications, additions or substitutions that those skilled in the art are made in the essential scope of the present invention
Without departure from spirit of the invention, it should also belong to the claims of the present invention.
Claims (9)
1. a kind of strain formula for administering black and odorous water, it is characterised in that：It includes following strains：Photosynthetic bacteria, bud pole bacterium,
Nitrosation bacillus, bacterium nitrobacter, denitrification bacillus, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism,
The complex microorganism includes pseudomonad, vibrios, Flavobacterium, the pure coccus of nitrous acid, thiobacillus thiooxidant, bacillus subtilis,
Bacillus licheniformis solves thiamine bud pole bacterium, the bacterium number of saccharomycete, denitrogenation coccus, actinomyces.
2. the strain formula according to claim 1 for administering black and odorous water, it is characterised in that：The complex microorganism includes
The raw material composition of following parts by weight：10~20 parts of pseudomonad, 10~15 parts of vibrios, 10~15 parts of Flavobacterium, nitrous acid is pure
10~15 parts of coccus, 10~15 parts of thiobacillus thiooxidant, 10~20 parts of bacillus subtilis, 5~15 parts of bacillus licheniformis solve sulphur
5~10 parts of amine element bud pole bacterium, 5~10 parts of saccharomycete, 5~10 parts of denitrogenation coccus, 5~10 parts of actinomyces.
3. the strain formula according to claim 2 for administering black and odorous water, it is characterised in that：The strain includes following weight
The group of amount number is grouped as：5~12 parts of photosynthetic bacteria, 20~30 parts of bud pole bacterium, 5~10 parts of Clostridium papyrosolvens, actinomyces 5~10
Part, 8~10 parts of Paracoccus denitrificans, 10~20 parts of nitrosation bacillus, 10~20 parts of bacterium nitrobacters, 10~20 parts of denitrification bacillus and
1~5 part of biological enzyme, 5~10 parts of Clostridium papyrosolvens, 8~10 parts of actinomyces, 1~5 part of Paracoccus denitrificans and complex microorganism 20~30
Part.
4. the strain formula according to claim 1 for administering black and odorous water, it is characterised in that：The bud pole bacterium is using withered
Careless bud pole bacterium and person's lichens bud pole bacterium.
5. the strain formula according to claim 4 for administering black and odorous water, it is characterised in that：Withered grass bud bubble bacillus and
The bacterium colony ratio of the lichens bud pole bacterium is 1：1-2：8.
6. the strain formula according to claim 1 for administering black and odorous water, it is characterised in that：The actinomyces include hammer
Bacterium.
7. the strain formula according to claim 1 for administering black and odorous water, it is characterised in that：The photosynthetic bacteria includes bath
Damp red pseudomonas.
8. the strain formula according to claim 1 for administering black and odorous water, it is characterised in that：The biological enzyme includes fat
Enzyme and subtilisin.
9. a kind of preparation method of strain formula that administering black and odorous water, it is characterised in that：Include the following steps：
S1：Configure culture medium：
S2：Inoculation：Photosynthetic bacteria, bud pole bacterium, nitrosation bacillus, bacterium nitrobacter, anti-is added in the culture medium prepared into S1
After bacterium nitrobacter, biological enzyme, Clostridium papyrosolvens, actinomyces, Paracoccus denitrificans and complex microorganism co-fermentation, filter is obtained by filtration
Liquid；
S3：With the filtrate prepared in protection liquid dilution S2.
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Cited By (7)
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| CN109576189A (en) * | 2018-12-29 | 2019-04-05 | 合肥市东方美捷分子材料技术有限公司 | A kind of black and odorous water composite bacteria agent and its preparation method and application |
| CN109678257A (en) * | 2019-03-01 | 2019-04-26 | 北京高能时代环境技术股份有限公司 | A method of being suitable for the functional flora construction method and application flora improvement black and odorous water of black and odorous water improvement |
| CN109880761A (en) * | 2019-03-07 | 2019-06-14 | 宜兴国际环保城科技发展有限公司 | It is a kind of for handling the composite bacteria of high-salt wastewater |
| CN110028166A (en) * | 2019-03-12 | 2019-07-19 | 宜兴国际环保城科技发展有限公司 | A kind of novel C OD remover |
| CN110317737A (en) * | 2019-05-28 | 2019-10-11 | 广东子辉生态环境科技有限公司 | Compound EM microorganism formulation, preparation method and application method |
| CN111925969A (en) * | 2020-09-07 | 2020-11-13 | 福泉环保城发展有限公司 | Water treatment microbial inoculum |
| CN113860520A (en) * | 2021-11-15 | 2021-12-31 | 重庆工商大学 | Preparation of black and odorous water body bottom mud remediation microbial inoculum and bottom mud in-situ ecological remediation method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109576189A (en) * | 2018-12-29 | 2019-04-05 | 合肥市东方美捷分子材料技术有限公司 | A kind of black and odorous water composite bacteria agent and its preparation method and application |
| CN109678257A (en) * | 2019-03-01 | 2019-04-26 | 北京高能时代环境技术股份有限公司 | A method of being suitable for the functional flora construction method and application flora improvement black and odorous water of black and odorous water improvement |
| CN109880761A (en) * | 2019-03-07 | 2019-06-14 | 宜兴国际环保城科技发展有限公司 | It is a kind of for handling the composite bacteria of high-salt wastewater |
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| CN110317737A (en) * | 2019-05-28 | 2019-10-11 | 广东子辉生态环境科技有限公司 | Compound EM microorganism formulation, preparation method and application method |
| CN111925969A (en) * | 2020-09-07 | 2020-11-13 | 福泉环保城发展有限公司 | Water treatment microbial inoculum |
| CN113860520A (en) * | 2021-11-15 | 2021-12-31 | 重庆工商大学 | Preparation of black and odorous water body bottom mud remediation microbial inoculum and bottom mud in-situ ecological remediation method |
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Application publication date: 20180803 |