CN108355168A - A kind of method that combination three-dimensional printing technology prepares nucleus pulposus time implant - Google Patents
A kind of method that combination three-dimensional printing technology prepares nucleus pulposus time implant Download PDFInfo
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- CN108355168A CN108355168A CN201810457291.5A CN201810457291A CN108355168A CN 108355168 A CN108355168 A CN 108355168A CN 201810457291 A CN201810457291 A CN 201810457291A CN 108355168 A CN108355168 A CN 108355168A
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- nucleus pulposus
- implant
- waste
- returns
- printing technology
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000010146 3D printing Methods 0.000 title claims abstract description 30
- 239000007943 implant Substances 0.000 title claims abstract description 27
- 238000005516 engineering process Methods 0.000 title claims abstract description 23
- 239000000463 material Substances 0.000 claims abstract description 47
- 239000002699 waste material Substances 0.000 claims abstract description 41
- 229920001610 polycaprolactone Polymers 0.000 claims abstract description 29
- 239000004632 polycaprolactone Substances 0.000 claims abstract description 29
- 238000007639 printing Methods 0.000 claims abstract description 24
- 150000001875 compounds Chemical class 0.000 claims abstract description 22
- 239000000806 elastomer Substances 0.000 claims abstract description 6
- 229920001971 elastomer Polymers 0.000 claims abstract description 6
- 230000003110 anti-inflammatory effect Effects 0.000 claims description 26
- 238000012545 processing Methods 0.000 claims description 20
- XMWRBQBLMFGWIX-UHFFFAOYSA-N C60 fullerene Chemical compound C12=C3C(C4=C56)=C7C8=C5C5=C9C%10=C6C6=C4C1=C1C4=C6C6=C%10C%10=C9C9=C%11C5=C8C5=C8C7=C3C3=C7C2=C1C1=C2C4=C6C4=C%10C6=C9C9=C%11C5=C5C8=C3C3=C7C1=C1C2=C4C6=C2C9=C5C3=C12 XMWRBQBLMFGWIX-UHFFFAOYSA-N 0.000 claims description 16
- 229910003472 fullerene Inorganic materials 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 7
- 239000011148 porous material Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 239000000155 melt Substances 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims 1
- 239000002504 physiological saline solution Substances 0.000 claims 1
- 238000009966 trimming Methods 0.000 claims 1
- 206010061246 Intervertebral disc degeneration Diseases 0.000 abstract description 5
- 208000018180 degenerative disc disease Diseases 0.000 abstract description 5
- 208000021600 intervertebral disc degenerative disease Diseases 0.000 abstract description 5
- 238000001356 surgical procedure Methods 0.000 abstract description 5
- 238000001035 drying Methods 0.000 abstract description 2
- 239000007787 solid Substances 0.000 abstract description 2
- 238000005457 optimization Methods 0.000 description 7
- 230000006835 compression Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 3
- 238000013138 pruning Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 108090001005 Interleukin-6 Proteins 0.000 description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 2
- 239000012267 brine Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 2
- 108010022355 Fibroins Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000003618 Intervertebral Disc Displacement Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 210000002805 bone matrix Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- PAPBSGBWRJIAAV-UHFFFAOYSA-N ε-Caprolactone Chemical compound O=C1CCCCCO1 PAPBSGBWRJIAAV-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
- A61L27/3645—Connective tissue
- A61L27/3654—Cartilage, e.g. meniscus
- A61L27/3658—Intervertebral discs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y50/00—Data acquisition or data processing for additive manufacturing
- B33Y50/02—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y80/00—Products made by additive manufacturing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/38—Materials or treatment for tissue regeneration for reconstruction of the spine, vertebrae or intervertebral discs
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Biomedical Technology (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Vascular Medicine (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Prostheses (AREA)
Abstract
The present invention relates to 3 D-printings to prepare nucleus pulposus go back to implant field, specially a kind of combination three-dimensional printing technology prepares the method that nucleus pulposus returns implant, this combines three-dimensional printing technology to prepare the method that nucleus pulposus returns implant, and mechanical support material of the polycaprolactone elasticity body support frame as nucleus pulposus implant is obtained by using 3 D-printing；Nucleus pulposus waste is obtained compound rest with polycaprolactone elastomer after treatment, and the holder mechanical property is good, and good biocompatibility, both include nucleus pulposus cell epimatrix and also include nucleus pulposus cell.Of the present invention kind of 3 D-printing prepares the polycaprolactone elastomer support product that nucleus pulposus returns the available different size for printing seriation first of method of implant treatment intervertebral disc degeneration, and product is drying solid, it is easy to preserve, long shelf-life, at the same the nucleus pulposus waste and polycaprolactone elasticity body support frame it is compound after Hui Zhi method can quickly handle in the course of surgery after Hui Zhi.
Description
Technical field
The present invention relates to 3 D-printings to prepare nucleus pulposus go back to implant field, and specially a kind of combination three-dimensional printing technology prepares marrow
The method that core returns implant.
Background technology
With aging of population and people's work, living-pattern preservation, Degenerative disc disease is more and more, especially
It is that neck and shoulder ache, pain in waist and lower extremities caused by disc herniation etc. are very common, serious patient often needs operative treatment, disc material to cut
Except being the commonly used operation method of spinal surgery, however ideal reconstruction method is never had after discectomy.With
The rapid development of tissue engineering technique, disc tissue engineering brings for the permanent reparation of hand Postoperative Intervertebral defect can
Energy.For this purpose, artificial intervertebral disk repair materials are the direction places studied at present, but due to biocompatibility, nucleus pulposus cell regeneration
The deficiencies of difficult, at present still no suitable material can really be used for the treatment of intervertebral disc degeneration, keep its recovery original
Physiological function.
In recent years, more for building the nucleus pulposus timbering material research of organizational project interverbebral disc.Such as polycaprolactone apple
Sour triol/decalcified bone matrix gelatin bi-phasic fiber ring support and polycaprolactone holder, but these man-made support bio-compatibles
Property mutually go victory remote with autologous tissue.In order to improve its biocompatibility, there is scholar to utilize fibroin material, gelfoam material
Material and nucleus pulposus acellular matrix material, but these materials do not have because itself mechanics is poor and not comprising nucleus pulposus cell
There is bionical nucleus pulposus well and reaches ideal effect.
In recent years, research report thinks, the nucleus pulposus waste being removed during surgery can be returned through over cleaning, anti-inflammatory processing
It plants to original position, and continues to play its biological action, promote the reparation of nucleus pulposus.But nucleus pulposus is easy to scatter in gelatin, after processing
Its original-shape is cannot keep, with less mechanical property.The rise of three-dimensional printing technology is to prepare the group of certain planform
Weaver's engineering support brings facility, because having good controllable appearance pattern and power by 3 D-printing polycaprolactone holder
Learn performance.Compound by carrying out the nucleus pulposus waste of 3 D-printing polycaprolactone holder and anti-inflammatory processing, then Hui Zhi is to former
Potential must have the clinical application potentiality of preferable treatment intervertebral disc degeneration, and at present still without relevant report.
Invention content
Present invention solves the technical problem that being to overcome the deficiencies of existing technologies, a kind of combination three-dimensional printing technology system is provided
The method that standby nucleus pulposus returns implant.
To achieve the above object, the present invention provides the following technical solutions：
A kind of method that combination three-dimensional printing technology prepares nucleus pulposus time implant, this prepares nucleus pulposus in conjunction with three-dimensional printing technology and returns
The method of implant includes the following steps：
The preparation of S1, elastic body support frame：
One, the selection of material：Appropriate pla-pcl material is measured, next step is waited for；
Two, the foundation of threedimensional model：Using 3 D-printing software building threedimensional model, then threedimensional model is exported, and
It preserves, waits for next step；
Three, the printing of threedimensional model：The pla-pcl material measured in step 1 is added to the material of 3D printing equipment
Cylinder is internal, then heats the pla-pcl material in barrel, after pla-pcl material melts completely, starts printing journey
Pla-pcl material is printed according to the threedimensional model established in step 2 by 3D printing equipment, is gathered by sequence
Caprolactone elasticity body support frame, wherein elastic support are to wait for next step with certain pore structure；
Four, the processing of elastic body support frame：It will be made by the pla-pcl material that obtained in step 3 using Co 60 ray
Elastic body support frame carries out irradiation sterilization, and elastic body support frame is placed in the environment of dry cryogenic sterile and is preserved, and waits for next step；
The processing of S2, nucleus pulposus waste：
One, the acquisition of nucleus pulposus waste：The nucleus pulposus waste of patient is taken out from patient's body by operation, etc.
Wait for next step；
Two, the flushing of nucleus pulposus waste：The patient disc's nucleus pulposus waste for acquisition of performing the operation in step 1 is passed through into physiology
Brine is rinsed, repeated multiple times, waits for next step；
Three, nucleus pulposus waste shreds：Nucleus pulposus waste in step 2 is crushed, next step is waited for；
Four, nucleus pulposus waste is anti-inflammatory：The anti-inflammatory material of fullerene is measured, the anti-inflammatory material of the fullerene of measurement is filtered
Then the anti-inflammatory material of fullerene is added the nucleus pulposus waste crushed into step 3 and is stirred by sterilization treatment, wait for next
Step；
The processing of S3, elastic body support frame：The nucleus pulposus waste obtained in S2 steps is injected into the bullet obtained in S1 steps
Property body support frame pore structure in, to form nucleus pulposus/polycaprolactone compound rest, wait for next step；
The reset of S4, compound rest：By the interverbebral disc of the nucleus pulposus obtained in S3/polycaprolactone compound rest Hui Zhi to patient
It is in situ.
Preferably, in the S1 3D printing equipment of step 3 a diameter of 150-400 μm of print head, and printed
Air pressure in journey in barrel is 600-1000kpa, floor height is 150-400 μm and printing path angle be 0/60,60/120 or
0/60/120。
Preferably, it is 5- that the threedimensional model of step 2, which is cylindrical shape, a diameter of 3-6mm and height, in the S1
The threedimensional model of 10mm.
Preferably, the export of the threedimensional model of step 2 is stl formatted files in the S1.
Preferably, the barrel heating temperature range in the S1 in step 3 is 100-150 DEG C.
Preferably, the dosage of the anti-inflammatory color material of fullerene of step 4 is 1-5mg in the S1.
Preferably, a length of 0.5-1h when total operation of the S2 steps and S3 steps.
Preferably, before returning plant nucleus pulposus/polycaprolactone compound rest, nucleus pulposus/polycaprolactone is compound in the S4 steps
Holder carries out pruning modes, the interverbebral disc of itself and patient is made to coincide.
Compared with prior art, the beneficial effects of the invention are as follows：This combines three-dimensional printing technology to prepare nucleus pulposus and returns implant
Method obtains mechanical support material of the polycaprolactone elasticity body support frame as nucleus pulposus implant by using 3 D-printing；Nucleus pulposus
Waste is obtained compound rest with polycaprolactone elastomer after treatment, and the holder mechanical property is good, and biocompatibility
It is good, both included nucleus pulposus cell epimatrix or includes nucleus pulposus cell.Of the present invention kind of 3 D-printing prepares nucleus pulposus time implant and controls
The polycaprolactone elastomer support product of the different size of the available seriation of printing first of method of intervertebral disc degeneration is treated, and is produced
Product are drying solid, are easy to preserve, long shelf-life, while the nucleus pulposus waste and the compound rear Hui Zhi of polycaprolactone elasticity body support frame
Method can quickly handle in the course of surgery after Hui Zhi.
Description of the drawings
Fig. 1 is that the 3 D-printing nucleus pulposus of the present invention returns implant preparation flow figure；
Fig. 2 is the circulating pressure Mechanical test results figure of the present invention；
Fig. 3 is the anti-inflammatory inflammatory experimental result of nucleus pulposus before and after the processing of the present invention.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- 3 are please referred to Fig.1, the present invention provides a kind of technical solution：
A kind of method that combination three-dimensional printing technology prepares nucleus pulposus time implant, this prepares nucleus pulposus in conjunction with three-dimensional printing technology and returns
The method of implant includes the following steps：
The preparation of S1, elastic body support frame：
One, the selection of material：Appropriate pla-pcl material is measured, next step is waited for；
Two, the foundation of threedimensional model：Using 3 D-printing software building threedimensional model, then threedimensional model is exported, and
It preserves, waits for next step；
Three, the printing of threedimensional model：The pla-pcl material measured in step 1 is added to the material of 3D printing equipment
Cylinder is internal, then heats the pla-pcl material in barrel, after pla-pcl material melts completely, starts printing journey
Sequence is printed pla-pcl material according to the threedimensional model established in step 2 by 3D printing equipment, until beating
Print is completed to obtain pla-pcl elasticity body support frame, and wherein elastic support is to wait for next step with certain pore structure；
Four, the processing of elastic body support frame：It will be made by the pla-pcl material that obtained in step 3 using Co 60 ray
Elastic body support frame carries out irradiation sterilization, until after the completion of sterilizing, elastic body support frame is placed in the environment of dry cryogenic sterile and is preserved,
Wait for next step；
The processing of S2, nucleus pulposus waste：
One, the acquisition of nucleus pulposus waste：The nucleus pulposus waste of patient is taken out from patient's body by operation, etc.
Wait for next step；
Two, the flushing of nucleus pulposus waste：The patient disc's nucleus pulposus waste for acquisition of performing the operation in step 1 is passed through into physiology
Brine is rinsed, repeated multiple times, until rinsing well, waits for next step；
Three, nucleus pulposus waste shreds：The nucleus pulposus waste rinsed well in step 2 is fully crushed, is waited in next step
Suddenly；
Four, nucleus pulposus waste is anti-inflammatory：The anti-inflammatory material of fullerene is measured, the anti-inflammatory material of the fullerene of measurement is filtered
Then the anti-inflammatory material of fullerene after filtration sterilization is added the nucleus pulposus waste crushed into step 3 and stirred by sterilization treatment
It mixes, until being sufficiently mixed, waits for next step；
The processing of S3, elastic body support frame：The nucleus pulposus waste obtained in S2 steps is injected into the bullet obtained in S1 steps
Property body support frame pore structure in, to form nucleus pulposus/polycaprolactone compound rest, wait for next step；
The reset of S4, compound rest：By the interverbebral disc of the nucleus pulposus obtained in S3/polycaprolactone compound rest Hui Zhi to patient
It is in situ.
As a kind of technical optimization scheme of the present invention, the print head diameter of the 3D printing equipment of step 3 in the S1
It it is 150-400 μm, and the air pressure in print procedure in barrel is 600-1000kpa, floor height is 150-400 μm and print path
Diameter angle is 0/60,60/120 or 0/60/120.
As a kind of technical optimization scheme of the present invention, in the S1 threedimensional model of step 2 be shape it is cylindrical,
A diameter of 3-6mm and the threedimensional model that height is 5-10mm.
As a kind of technical optimization scheme of the present invention, the export of the threedimensional model of step 2 is stl in the S1
Formatted file.
As a kind of technical optimization scheme of the present invention, the barrel heating temperature range in the S1 in step 3 is 100-
150℃。
As a kind of technical optimization scheme of the present invention, the dosage of the anti-inflammatory color material of fullerene of step 4 is 1- in the S1
5mg。
As a kind of technical optimization scheme of the present invention, when total operation of the S2 steps and S3 steps a length of 0.5-
1h。
As a kind of technical optimization scheme of the present invention, in the S4 steps, nucleus pulposus/polycaprolactone compound rest is planted returning
Before, nucleus pulposus/polycaprolactone compound rest is subjected to pruning modes, the interverbebral disc of itself and patient is made to coincide.
Embodiment one
Using 3 D-printing software building threedimensional model, shape is cylindrical, a diameter of 3mm, is highly 5mm.Export stl
Formatted file simultaneously preserves.Polycaprolactone is added in the barrel of 3 D-printing, is heated to 100-150 DEG C, after complete melting,
Start print routine and carries out printing shaping.A diameter of 150 μm of print head, barrel air pressure is 800KPa in print procedure, and floor height is
150μm.Printing path angle is 0/60.The polycaprolactone elasticity body support frame that printing is obtained is sterilized simultaneously using Co 60 x ray irradiation x
It is stored under dry cryogenic sterile environment.
The nucleus pulposus waste taken out in operation is rinsed, is shredded, anti-inflammatory processing.Rinsing step discards nucleus pulposus
Object is clean using normal saline flushing, and is repeated as many times.Step is shredded, the nucleus pulposus waste is fully shredded.Anti-inflammatory processing
The anti-inflammatory material of fullerene is added in nucleus pulposus waste and fully stirs evenly by step.The anti-inflammatory material of the fullerene needs mistake in advance
Filter sterilization processing, addition 5mg.
In surgical procedure, by treated, nucleus pulposus waste is injected into the hole of polycaprolactone elasticity body support frame, is obtained
Nucleus pulposus/polycaprolactone compound rest.Whole process about 1h.
After carrying out pruning modes to nucleus pulposus/polycaprolactone compound rest according to actual size, by its Hui Zhi to interverbebral disc original
Position.
Embodiment two
Radial mechanical performance test is carried out to printing gained 3 D-printing elasticity body support frame in embodiment 1, which uses
Omnipotent mechanical test instrument radially carries out cycle compression to holder, and largest deformation is 60% when test, compression speed 10cm/
Min carries out 100 cycles altogether.Ess-strain cyclic curve by the compression up to 100 times as shown in Fig. 2, recycle, the holder
Still maintain preferable mechanical property.
Embodiment three
Nucleus pulposus waste is anti-inflammatory before and after the processing in addition fullerene in performing the operation in above-described embodiment 1, utilizes ELASA reagents
Box detects the content of TNF-α and IL-6, and does block diagram.
Anti-inflammatory experimental result is as shown in figure 3, the TNF-α of nucleus pulposus group and the content of IL-6 are significantly lower than anti-after anti-inflammatory processing
Nucleus pulposus group before inflammation processing, this shows that the nucleus pulposus added with fullerene plays anti-inflammatory effect, reduces the inflammatory levels in nucleus pulposus.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of combination three-dimensional printing technology prepares the method that nucleus pulposus returns implant, it is characterised in that：This combines three-dimensional printing technology
The method that nucleus pulposus returns implant is prepared to include the following steps：
The preparation of S1, elastic body support frame：
One, the selection of material：Appropriate pla-pcl material is measured, next step is waited for；
Two, the foundation of threedimensional model：Using 3 D-printing software building threedimensional model, then threedimensional model is exported, and preserves,
Wait for next step；
Three, the printing of threedimensional model：The pla-pcl material measured in step 1 is added to the barrel of 3D printing equipment
Then portion heats the pla-pcl material in barrel, after pla-pcl material melts completely, start print routine,
Pla-pcl material is printed according to the threedimensional model established in step 2 by 3D printing equipment, is obtained poly- interior
Ester elastomer holder, wherein elastic support are to wait for next step with certain pore structure；
Four, the processing of elastic body support frame：Using Co 60 ray by the elasticity made by the pla-pcl material obtained in step 3
Body support frame carries out irradiation sterilization, and elastic body support frame is placed in the environment of dry cryogenic sterile and is preserved, and waits for next step；
The processing of S2, nucleus pulposus waste：
One, the acquisition of nucleus pulposus waste：The nucleus pulposus waste of patient is taken out from patient's body by operation, under waiting
One step；
Two, the flushing of nucleus pulposus waste：The patient disc's nucleus pulposus waste for acquisition of performing the operation in step 1 is passed through into physiological saline
It is rinsed, it is repeated multiple times, wait for next step；
Three, nucleus pulposus waste shreds：Nucleus pulposus waste in step 2 is crushed, next step is waited for；
Four, nucleus pulposus waste is anti-inflammatory：The anti-inflammatory material of fullerene is measured, the anti-inflammatory material of the fullerene of measurement is filtered sterilizing
Processing, is then added the nucleus pulposus waste crushed into step 3 by the anti-inflammatory material of fullerene and is stirred, and waits for next step；
The processing of S3, elastic body support frame：The nucleus pulposus waste obtained in S2 steps is injected into the elastomer obtained in S1 steps
In the pore structure of holder, to form nucleus pulposus/polycaprolactone compound rest, next step is waited for；
The reset of S4, compound rest：The interverbebral disc of the nucleus pulposus obtained in S3/polycaprolactone compound rest Hui Zhi to patient is former
Position.
2. a kind of combination three-dimensional printing technology according to claim 1 prepares the method that nucleus pulposus returns implant, it is characterised in that：
A diameter of 150-400 μm of the print head of the 3D printing equipment of step 3 in the S1, and the gas in print procedure in barrel
Pressure is 600-1000kpa, floor height is 150-400 μm and printing path angle is 0/60,60/120 or 0/60/120.
3. a kind of combination three-dimensional printing technology according to claim 1 prepares the method that nucleus pulposus returns implant, it is characterised in that：
The threedimensional model of step 2 is the threedimensional model that cylindrical shape, a diameter of 3-6mm and height are 5-10mm in the S1.
4. a kind of combination three-dimensional printing technology according to claim 1 prepares the method that nucleus pulposus returns implant, it is characterised in that：
The export of the threedimensional model of step 2 is stl formatted files in the S1.
5. a kind of combination three-dimensional printing technology according to claim 1 prepares the method that nucleus pulposus returns implant, it is characterised in that：
Barrel heating temperature range in the S1 in step 3 is 100-150 DEG C.
6. a kind of combination three-dimensional printing technology according to claim 1 prepares the method that nucleus pulposus returns implant, it is characterised in that：
The dosage of the anti-inflammatory color material of the fullerene of step 4 is 1-5mg in the S1.
7. a kind of combination three-dimensional printing technology according to claim 1 prepares the method that nucleus pulposus returns implant, it is characterised in that：
A length of 0.5-1h when total operation of the S2 steps and S3 steps.
8. a kind of combination three-dimensional printing technology according to claim 1 prepares the method that nucleus pulposus returns implant, it is characterised in that：
In the S4 steps, before returning plant nucleus pulposus/polycaprolactone compound rest, nucleus pulposus/polycaprolactone compound rest is carried out at trimming
Reason makes the interverbebral disc of itself and patient coincide.
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