CN108324829A - A kind of Hemorrhagic shock mixture and preparation method thereof containing ginseng - Google Patents

Abstract

The invention discloses an anti-shock mixture containing ginseng. The raw materials for its preparation are composed of the following components in parts by weight: 20-30 parts of ginseng, 10-20 parts of Aesculus aesculata, 10-20 parts of green bark, and Herba Cervix 5‑15 parts, 5‑15 parts of Solanum nigrum, 5‑10 parts of Ling Xiaohua, 3‑5 parts of Ruyi grass. The invention also provides a preparation method of the anti-shock mixture containing ginseng. The anti-shock mixture of the present invention has the effects of nourishing qi, nourishing yin and generating blood, warming yang, removing blood stasis and dredging collaterals, replenishing qi and invigorating the spleen, can significantly improve heart function, restore blood pressure and heart rate to normal levels, and has a good curative effect on hemorrhagic shock . In addition, the anti-shock mixture of the present invention is made from natural Chinese medicine raw materials, the medicinal materials are reasonably compatible, conforms to the research theory of Chinese medicine and modern medicine, and can achieve the purpose of treating both symptoms and root causes, and the preparation process of the present invention is simple, the drug effect is stable, and it is easy to popularize and apply. It has a good application prospect.

Description

A kind of anti-shock mixture containing ginseng and its preparation method

technical field

The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to an anti-shock mixture containing ginseng and a preparation method thereof.

Background technique

Shock is a systemic systemic disorder in which the effective circulating blood volume decreases sharply and tissue blood perfusion is seriously insufficient under the action of severe blood loss, fluid loss, infection, trauma and other strong pathogenic factors, leading to various important vital organs, cellular dysfunction and structural damage. pathological process. Shock is a common clinical emergency. Although there are many causes and complicated pathogenesis, microcirculatory disturbance is the common basis for polymorphic shock. During the development of shock, the body produces a series of pathophysiological changes, mainly including decreased cardiac contractility, decreased microcirculatory blood volume, abnormal blood flow distribution, decreased oxygen supply, obvious ischemia-reperfusion injury, and inflammatory mediators. In this pathological process, they transform each other and cause and effect each other, leading to energy generation disorders, tissue ischemia, hypoxia, activation of neurohumoral mechanisms, and various disturbances in body metabolism and functions, which may eventually lead to systemic inflammatory reactions syndrome (SIRS), multiple organ dysfunction (MODS), and even death.

Shock caused by massive blood loss is called hemorrhagic shock. It is common in bleeding caused by trauma, peptic ulcer bleeding, rupture of esophageal varices, bleeding caused by obstetrics and gynecology diseases, etc. Whether shock occurs after blood loss depends not only on the amount of blood loss, but also on the speed of blood loss. Shock often occurs when blood is lost rapidly and in large quantities (more than 30%-35% of the total blood volume) and cannot be replenished in time of.

The drug treatment in the current market still takes western medicine as the main means. Although there are many traditional Chinese medicine preparations, it is difficult to ensure their safety and therapeutic effect, and there are very few effective effects.

Ginseng is a plant of the genus Panax of Araliaceae. The ginsenoside contained in ginseng can significantly prolong the survival time of dogs with hemorrhagic shock, prevent damage to the sarcolemma, nuclear membrane, and mitochondria of myocardial cells in hemorrhagic shock, and protect the heart, Brain, kidney and liver, but existing studies only show that ginseng saponins and ginsenoside Re contained in ginseng have a certain therapeutic effect on hemorrhagic shock, and there is no report on the treatment of hemorrhagic shock with ginsenoside Rb1; Xu Hongping and others studied the protective effect of ginsenoside Rb1 on the vital organs of septic shock rats (Journal of Zhengzhou University. May 2014 Vol.49 No.3), and proved that Rb1 can protect the liver and lung tissues of septic shock rats, and The mechanism may be related to reducing the expression level of TLR4 mRNA and inhibiting the production of TNF-α. Similarly, the literature did not study the therapeutic effect of ginsenoside Rb1 on hemorrhagic shock.

The invention adopts a novel and efficient extraction method to extract the ginsenoside Rb1 contained in the ginseng and use it in combination with other traditional Chinese medicine raw materials. Animal experiments prove that the anti-shock mixture of the invention has a good therapeutic effect on hemorrhagic shock.

Contents of the invention

The invention provides an anti-shock mixture containing ginseng, which solves the problem that in the prior art, western medicine is still the main means of drug treatment in the market, and although there are many traditional Chinese medicine preparations, it is difficult to ensure the safety and therapeutic effect.

The first object of the present invention is to provide a ginseng-containing anti-shock mixture, the preparation raw materials of which are composed of the following components in parts by weight: 20-30 parts of ginseng, 10-20 parts of Aescin, 10-20 parts of Qingpi 5-15 parts of Cervus nigra, 5-15 parts of Solanum nigrum, 5-10 parts of Ling Xiaohua, 3-5 parts of Ruyi grass.

Preferably, the raw materials for the preparation of the ginseng-containing anti-shock mixture consist of the following components in parts by weight: 25 parts of ginseng, 15 parts of Echinacea chinensis, 15 parts of green bark, 10 parts of deerbite, 10 parts of black nightshade, 5 parts of Lingxiaohua and 5 parts of Ruyicao.

The second object of the present invention is to provide a preparation method of anti-shock mixture containing ginseng, comprising the following steps:

Step 1, take by weight 20-30 parts of ginseng, 10-20 parts of Aescin, 10-20 parts of green bark, 5-15 parts of cervix, 5-15 parts of black nightshade, 5-10 parts of Ling Xiaohua, 3-5 parts of wishful grass;

Step 2, drying and crushing the ginseng weighed in step 1, passing through a 20-mesh sieve to obtain ginseng powder; mixing ginseng powder, water, and lignin peroxidase in a mass ratio of 100:50:1, and then placing Enzyme hydrolyze at 60°C for 3 hours, and the enzymolysis product is obtained after enzymolysis; filter the enzymolysis product to obtain the first filtrate and filter residue, add an ethanol solvent equivalent to 5 times the weight of the filter residue to the filter residue for ultrasonic extraction, and filter after the extraction is completed. Obtain the second filtrate, combine the first filtrate and the second filtrate, and concentrate under reduced pressure to a relative density of 1.3-1.4 to obtain a ginseng extract;

Mix and dissolve the ginseng extract and water according to the ratio of 1g: 10mL, pass it through a silica gel column, perform silica gel column chromatography at a column pressure of -0.5Mpa, and use a mixed solution of ethanol and ethyl acetate as an eluent to wash Take off, collect fractions and concentrate, dry, obtain ginsenoside crude product;

The crude ginsenoside and n-butanol were mixed and dissolved according to the ratio of 1g:5mL, then injected into a high-performance preparative liquid chromatograph, eluted with a mixed solution of ethanol and water, and the fractions were collected, concentrated, and dried to obtain ginsenoside Rb1;

Step 3, drying and pulverizing the Aesculus aesculus flower weighed in step 1 and passing through a 20-mesh sieve to obtain Aesculus Aesculus pollen, adding 5% Aspergillus aculeatus culture solution equivalent to its weight to the Aesculus aesculata pollen, Ferment for 5 days under the condition of 28-30°C, and the fermentation product is obtained after the fermentation is completed;

Mix the fermentation product with ethanol aqueous solution at a ratio of 1 g: 5 mL, then reflux at 80°C for 3 hours, filter after the reflux is completed, and concentrate the filtrate to a relative density of 1.3-1.4 to obtain the Aescinus aesculus extract;

Step 4, mix the green bark, chrysanthemum, black nightshade, lingxiaohua, and ruyicao weighed in step 1 to obtain a mixture, then add ethanol equivalent to 5 times the weight of the mixture to the mixture, heat and reflux for 3 hours, and then filter , to obtain the filtrate, the filtrate was concentrated under reduced pressure to remove ethanol, and a mixed extract was obtained;

Step 5, mix ginsenoside Rb1 in step 2, Aesculus aesculus extract in step 3 and mixed extract in step 4, and then add the total weight of ginsenoside Rb1, Aesculus aesculus extract and mixed extract The equal sucrose aqueous solution is mixed evenly to obtain the anti-shock mixture.

Preferably, the ultrasonic extraction conditions in step 2 are 50°C, 200w power, and 30min ultrasonic extraction.

Preferably, the enzyme activity of the lignin peroxidase is 10000U/mL.

Preferably, the diameter-to-height ratio of the silica gel column is 1:5.

Preferably, the number of effective viable bacteria in the Aspergillus aculeatus culture solution is ≥10 ¹⁰ CFU/mL.

Preferably, the mass concentration of the sucrose aqueous solution is 80%.

Pharmacological action and drug effect of each raw material Chinese medicine drug in the present invention:

1. Ginseng: flat in nature, sweet in taste, slightly bitter, slightly warm. Return spleen, lung, heart channel. It has the effects of invigorating vital energy, recovering veins and strengthening the spleen, nourishing the spleen and lungs, promoting body fluid and quenching thirst, calming the nerves and improving intelligence. "Shen Nong's Materia Medica" clearly records that "Ginseng mainly nourishes the five internal organs, soothes the spirit, calms the soul, relieves palpitations, eliminates evil spirits, improves eyesight, improves intelligence, makes light of body and prolongs life after long-term use." Ginsenoside is one of the main active ingredients of ginseng. Ginsenoside Rb1 in ginsenoside has protective effect on lipopolysaccharide-induced mesenteric microcirculation disorder in rats, has protective effect on acute renal tubular injury in rats, and can promote rabbit chondrocyte proliferation , Inhibit the expression of nitric oxide synthase in melanocytes, have a repairing effect on central nervous system damage, and have a better protective effect on ischemia-reperfusion brain tissue damage.

2, Aescin aesculus: bitter, cool. Small poison. GUIXIN, liver, lung, stomach, large intestine channel. It has the effects of detoxifying and anticancer, reducing swelling and relieving pain, clearing heat and calming convulsions, relieving cough and asthma. The rhizome of Aescin contains 18 kinds of amino acids such as steroidal saponins and alanine, and creatinine and other components. One of the main components of Yunnan Baiyao; in recent years, it has been found that its chemical component diosgenin has good antitumor, antibacterial, anti-inflammatory and therapeutic effects on gastric ulcer, leukemia, cardiovascular disease, sedative and analgesic, cough and asthma, and sperm suppression. and other physiological activities.

3, Qingpi: bitter, pungent, warm in nature. Return liver, gallbladder, stomach warp. It has the effects of soothing the liver and breaking qi, eliminating accumulation and stagnation. It is used for distending pain in the chest and hypochondrium, hernia pain, stagnation of Qi due to indigestion, abdominal distension and pain. Experimental studies have shown that Qingpi has the strongest effect on relaxing gastrointestinal smooth muscle, and the mechanism is to block M receptors, excite α receptors, and directly inhibit intestinal smooth muscle. In addition, Qingpi has a longer maintenance time for boosting blood pressure, can excite breathing, and has the effects of anti-shock and exciting the heart.

4, Herba Cervix: sweet, bitter, warm. Return lung, stomach, liver, kidney channel. It has the effects of tonifying deficiency, nourishing kidney, expelling wind and dampness, promoting blood circulation and regulating menstruation, tonifying kidney and strengthening bones, relieving cough and stopping bleeding. Indications for lumbago due to kidney deficiency, rheumatic arthralgia, flaccidity of muscles and bones, chronic cough, hematemesis, epistaxis, uterine bleeding, traumatic bleeding and other diseases. Wintergreen can increase myocardial contractility, resist arrhythmia, increase myocardial contractility, significantly dilate blood vessels, and lower blood pressure. Injectable infusions of wintergreen also counteract the vasoconstrictive effects of norepinephrine, causing it to dilate. The water decoction of Luliancao can significantly increase the nutritional blood flow of the mouse myocardium and tissue (liver, kidney, spleen, brain) blood flow, and can significantly increase the plasma cAMP content. In addition, it has inhibitory effect on Staphylococcus aureus, hemolytic streptococcus, pneumococcus, meningococcus, Shigella flexneri, Salmonella typhi and Pseudomonas aeruginosa.

5. Solanum nigrum: cold in nature, bitter in taste, slightly sweet, slightly poisonous. It has the effects of clearing away heat, detoxification and diuresis. For sores, carbuncles, swollen toxins, skin eczema, dysuria, senile chronic bronchitis, excessive leucorrhea, prostatitis, and dysentery. It has an anti-inflammatory effect on animals, and the Australian solanamine contained in it can reduce the vascular permeability and the activity of hyaluronidase; it has certain protective effects on animals' anaphylaxis, burns, and histamine shock, and can also increase the The survival rate of insulin shock, and can promote the formation of antibodies. Solanum nigrum has a certain antibacterial effect on Staphylococcus aureus, Shigella, Salmonella typhi, Proteus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus choleraesuis.

6. Lingxiaohua: pungent, sour, cold in nature. Return liver, pericardium channel. It has the effects of promoting blood circulation to remove blood stasis, cooling blood and expelling wind. For amenorrhea, postpartum breast swelling, rubella redness, skin itching, acne. The flavonoids contained in Ling Xiaohua can speed up the blood flow of animals, increase the intersection of capillary networks, expand the diameter of small blood vessels, and have a good effect on improving microcirculation, promoting blood circulation and removing blood stasis.

7. Ruyicao: pungent, slightly sour, cold in nature. It has the effects of clearing away heat and detoxification, dissipating phlegm and stopping bleeding. Mainly treat sores and swelling, mastitis, bruises, open fractures, traumatic bleeding, and snakebites.

Traditional Chinese medicine believes that the etiology of hemorrhagic shock can be summarized into the following two aspects: 1. Depletion of yin and blood due to prolonged illness and depletion of true yin, or yin and blood caused by blood loss, sweating, vomiting, diarrhea, and excessive labor. Severe injury, the viscera are lost in nourishment; or the evil of the six evils caused by exogenous diseases enters the interior to transform heat, the heat and toxins are blazing, and the Yin fluid is consumed. Yang does not grow without yin, and yin damage will affect yang, resulting in yin deficiency and yang loss. Yang Qi loses its warmness and leads to Jue Syndrome. In severe cases, Yang Qi has nothing to attach to, and Yang Qi is deficient and external, resulting in loss of syndrome. 2. Decreased yang qi due to prolonged illness or severe illness that injures yang and consumes qi, leading to great decline of yang qi, or yin damage to yang, deficiency and deficiency of yang qi that cannot be warmed, resulting in syncope. If the yang qi declines, and the yang is not attached to the yin and falls off, it will lead to detachment. Traditional Chinese medicine adopts syndrome differentiation and classification. Jue syndrome is mainly based on benefiting Qi and yang, dispelling blood stasis and dredging collaterals.

The traditional Chinese medicine prescription of the present invention is based on the recognition of syndrome differentiation of Chinese medicine on shock, follows the compatibility theory of traditional Chinese medicine, and in order to make a convenient and easy-to-use preparation with stable curative effect, the usage amount of medicinal materials is strengthened. This formula uses ginseng and Aescin as the monarch drug, dispelling cold and relieving pain, warming the meridians to stop bleeding, dispelling wind and dampness, clearing numbness and relieving pain; taking green skin and deer's grass as minister drugs, tonifying qi and strengthening the surface, cooling blood and stopping bleeding , nourishing yin and blood, nourishing essence and filling the marrow; using Solanum nigrum as an adjuvant drug, clearing the orifices and relieving pain, promoting blood circulation and stimulating the menstrual flow, replenishing qi and promoting body fluid, nourishing the middle and replenishing qi, nourishing blood and calming the nerves; taking lingxiaohua and ruyicao as envoys to reconcile the properties of various medicines , the combination of various medicines, complement each other, and synergistically play the effects of nourishing qi, nourishing yin and generating blood, warming yang, dispelling blood stasis and dredging collaterals, replenishing qi and invigorating the spleen, etc., and has a very significant curative effect on shock.

Compared with prior art, the beneficial effect of the present invention is:

(1) The anti-shock mixture of the present invention has the effects of nourishing qi, nourishing yin and producing blood, warming yang, dispelling blood stasis and dredging collaterals, replenishing qi and invigorating the spleen, can significantly improve heart function, restore blood pressure and heart rate to normal levels, and has a certain effect on hemorrhagic shock. Better curative effect.

(2) The Chinese medicine mixture of the present invention is made from high-quality Chinese medicinal materials, and the selected medicinal materials are suitable in compatibility, conforms to the research theory of traditional Chinese medicine and modern medicine, can achieve the purpose of treating both symptoms and root causes, and the preparation process of the present invention is simple and easy, and the drug effect of the preparation is stable , easy to popularize and apply, and has good clinical application prospect.

Detailed ways

In order to enable those skilled in the art to better understand that the technical solutions of the present invention can be implemented, the present invention will be further described below in conjunction with specific examples, but the given examples are not intended to limit the present invention.

The Aspergillus aculeatus culture solution used in the examples was obtained by culturing by conventional methods, and the experimental methods described in the following examples, unless otherwise specified, were conventional methods.

Example 1

An anti-shock mixture containing ginseng, the preparation raw materials of which are composed of the following components in parts by weight: 25 parts of ginseng, 15 parts of Aescin, 15 parts of green bark, 10 parts of chrysanthemum, 10 parts of Solanum nigrum, 5 parts of Ling Xiaohua 5 parts, 5 parts of Ruyi grass.

Concretely prepare according to the following steps:

Step 1, take by weight 25 parts of ginseng, 15 parts of Aescin, 15 parts of green bark, 10 parts of pilus nigra, 10 parts of solanum nigrum, 5 parts of lingxiaohua, and 5 parts of ruyicao;

Step 2, drying and crushing the ginseng weighed in step 1, passing through a 20-mesh sieve to obtain ginseng powder; mixing ginseng powder, water, and lignin peroxidase in a mass ratio of 100:50:1, and then placing Enzymolysis at 60°C for 3 hours, the enzymolysis product was obtained after the enzymolysis was completed; the enzymolysis product was filtered to obtain the first filtrate and filter residue, and an ethanol solvent equivalent to 5 times the weight of the filter residue was added to the filter residue, and then at 50°C, ultrasonic power Ultrasonic extraction for 30 minutes under the condition of 200W, after the extraction is completed, filter to obtain the second filtrate, combine the first filtrate and the second filtrate, concentrate under reduced pressure to a relative density of 1.3-1.4, and obtain the ginseng extract;

Mix and dissolve the ginseng extract and water according to the ratio of 1g:10mL, pass through a silica gel column with a diameter-to-height ratio of 1:5, perform silica gel column chromatography at a column pressure of -0.5Mpa, and use ethanol and ethyl acetate The mixed solution is used as an eluent to elute, and the fractions are collected, concentrated and dried to obtain crude ginsenosides;

The crude ginsenoside and n-butanol were mixed and dissolved according to the ratio of 1g:5mL, then injected into a high-performance preparative liquid chromatograph, eluted with a mixed solution of ethanol and water, and the fractions were collected, concentrated, and dried to obtain ginsenoside Rb1;

Step 3, drying and pulverizing the Aesculus aesculus flower weighed in step 1 and passing through a 20-mesh sieve to obtain Aesculus Aesculus pollen, adding a culture solution of Aspergillus aculeatus equivalent to 5% of its weight in the Aesculus aesculata pollen, and Ferment at 28-30°C for 5 days, and the fermentation product is obtained after the fermentation is completed;

Mix the fermentation product with ethanol aqueous solution at a ratio of 1 g: 5 mL, then reflux at 80°C for 3 hours, filter after the reflux is completed, and concentrate the filtrate to a relative density of 1.3-1.4 to obtain the Aescinus aesculus extract;

Step 4, mix the green bark, chrysanthemum, black nightshade, lingxiaohua, and ruyicao weighed in step 1 to obtain a mixture, then add ethanol equivalent to 5 times the weight of the mixture to the mixture, heat and reflux for 3 hours, and then filter , to obtain the filtrate, the filtrate was concentrated under reduced pressure to remove ethanol, and a mixed extract was obtained;

Step 5, mix ginsenoside Rb1 in step 2, Aesculus aesculus extract in step 3 and mixed extract in step 4, and then add the total weight of ginsenoside Rb1, Aesculus aesculus extract and mixed extract The same sucrose aqueous solution with a mass concentration of 80% is uniformly mixed to obtain the anti-shock mixture.

Example 2

An anti-shock mixture containing ginseng, the preparation raw materials of which are composed of the following components in parts by weight: 20 parts of ginseng, 20 parts of aescin, 10 parts of green bark, 15 parts of chrysanthemum, 5 parts of solanum nigrum, and 8 parts of Ling Xiaohua 4 parts, 4 parts of Ruyi grass.

The preparation method is the same as that of Example 1, except that the formula in Example 1 is replaced with that of Example 2.

Example 3

An anti-shock mixture containing ginseng, the preparation raw materials of which are composed of the following components in parts by weight: 30 parts of ginseng, 10 parts of Aesculus chinensis, 20 parts of green bark, 5 parts of chrysanthemum, 15 parts of black nightshade, and 10 parts of Ling Xiaohua 3 servings, 3 servings of Ruyi Cao.

The preparation method is the same as that of Example 1, except that the formula in Example 1 is replaced with that of Example 3.

What needs to be said is that the enzyme activity of lignin peroxidase is 10000U/mL, and the number of effective viable bacteria in the Aspergillus aculeatus culture solution is ≥10 ¹⁰ CFU/mL.

In order to further illustrate the effect of the present invention, a comparative example is also set up in the experiment, as follows.

Comparative example 1

The formula and preparation method are the same as in Example 1, except that the formula does not contain ginseng.

Comparative example 2

The formula is the same as in Example 1, except that in the step 2 of the preparation method, the preparation method of the ginseng extract is as follows:

Dry and crush the ginseng weighed in step 1 and pass it through a 20-mesh sieve to obtain ginseng powder; mix ginseng powder, water, and lignin peroxidase in a mass ratio of 100:50:1, and place at 60°C Under enzymatic hydrolysis for 3 hours, the enzymolysis product is obtained after the enzymolysis is completed; the enzymolysis product is filtered to obtain the first filtrate and filter residue, and the ethanol solvent equivalent to 5 times the weight of the filter residue is added to the filter residue for ultrasonic extraction. After the extraction is completed, filter to obtain the second The filtrate, combining the first filtrate and the second filtrate, concentrating under reduced pressure to a relative density of 1.3-1.4 to obtain a ginseng extract;

Step 5, mix the ginseng extract in step 2, the extract of Aesculus aesculus in step 3 and the mixed extract in step 4, and then add the total weight of ginseng extract, Aesculus aesculus extract and the mixed extract The equal sucrose aqueous solution is mixed evenly to obtain the anti-shock mixture.

Comparative example 3

The formula and preparation method are the same as those in Example 1, except that the formula does not contain green bark, pilchard and black nightshade.

Comparative example 4

The formula and preparation method are the same as in Example 1, except that the formula does not contain Lingxiaohua and Ruyicao.

Examples 1-3 all prepared the anti-shock mixture containing ginseng with good effect. The following animal experiments are used to test the performance of the anti-shock mixture containing ginseng prepared in Examples 1-3 to illustrate the effect of the present invention .

1. Experimental method

Select 100 SPF grade SD rats, body weight is 280 ± 10g, half male and half male, randomly divided into normal control group, model group, positive control group, embodiment 1-3 group, comparative example 1-4 group, totally 10 groups , 10 in each group. Except for the normal control group, the rats in the other groups were weighed respectively, anesthetized with 1% pentobarbital sodium (35 mg/kg), fixed on a 37°C constant temperature blanket in the supine position, and the electrocardiogram electrodes were respectively connected to the lower limbs and the Right upper extremity to record the ECG waveform. After skin preparation and disinfection, a tracheotomy was performed, the trachea was intubated, and spontaneous breathing was retained. The vessels and nerves of the right neck and lower extremities were separated respectively. A 22G venous catheter was placed in the right carotid artery and connected to a MAP cardiac function analyzer to monitor the mean arterial pressure. The left femoral vein is used as a resuscitation channel to give vasoactive drugs and return fluid and blood. 1KU/kg heparin sodium was injected through the left femoral vein to heparinize the whole blood. A 24G venous catheter was inserted into the right femoral artery and connected to a precision infusion set, and blood was bled until shock.

The rats in the embodiment 1-3 group were given the anti-shock mixture prepared by the embodiment of the present invention 1-3 by gavage respectively, and the dosage was 20 mg/kg, and the rats were administered immediately after the shock; Rats were administered intragastrically to the anti-shock mixture prepared in Comparative Examples 1-4, and the dosage was 20 mg/kg, administered immediately after the shock; the positive control group was intragastrically administered epinephrine, and the dosage was 20 mg/kg. The normal control group and the model group were given the same volume of normal saline, and each group was gavaged once a day for 5 consecutive days.

In order to determine the therapeutic effect of the anti-shock mixture of the present invention on hemorrhagic shock, detect the heart rate and mean arterial pressure of the rats before shock, during shock and after shock respectively.

Statistical processing The experimental data are expressed as mean ± standard deviation, the significant difference between groups is compared by t test, and SPSS19.0 statistical software is used for data processing.

2. Test results

The test results are shown in Table 1-2.

Table 1 Effects on Rat Heart Rate (HR)

Note: Compared with the normal control group, *P<0.05; compared with the model group, **P<0.01.

As can be seen from Table 1, the decline in HR of the hemorrhagic shock model rats was the most significant when the shock was 30min, and all died after complete shock; Rat HR, to restore it to normal levels, its therapeutic effect is comparable to that of commercially available epinephrine.

Rat HR significantly decreased after gavage of comparative example 1 anti-shock mixture, and it was difficult to return to the normal level, indicating that the role played by ginseng in the whole anti-shock mixture is very important, lacking ginseng, although the anti-shock mixture has better therapeutic effect than Model group, but the effect is much worse than other groups.

After gavage of comparative example 2 anti-shock mixture, rat HR decreased more, although it was better than comparative example 1 and the model group, it was worse than other groups, indicating that ginsenoside Rb1 contained in ginseng is the main component for treating hemorrhagic shock, and , Traditional Chinese medicine components pay attention to compatibility. Compared with unpurified ginseng extract, pure ginsenoside Rb1 has a better compatibility effect with other raw material components.

After gavage of comparative example 3 and comparative example 4 anti-shock mixture, the reduction in rat HR is less, and the effect is better than that of comparative example 1 and comparative example 2, but worse than that of examples 1-3. Both Lingxiaohua and Ruyicao play their respective roles in the whole system, and under the compatibility of the present invention, the raw materials interact with each other, so that the effect of the prepared anti-shock mixture reaches the best.

The impact of table 2 on rat mean arterial pressure (MAP)

group Dose (mg/kg) Before shock (mmHg) Shock 30min(mmHg) After shock (mmHg) Example 1 20 113±21 78±10** 92±12* Example 2 20 115±18 76±12** 98±11* Example 3 20 112±19 82±8** 95±11* Comparative example 1 20 111±22 58±12** 65±9* Comparative example 2 20 113±20 64±10** 76±8* Comparative example 3 20 113±19 71±11** 89±11* Comparative example 4 20 114±18 74±12** 90±13* normal control group / 112±21 112±17 112±17 model group / 116±28 42±8* all dead positive control group 20 115±23 69±10** 93±12*

Note: Compared with blank control group, *P<0.05; compared with model group, **P<0.01.

As can be seen from Table 2, the decline in MAP of the hemorrhagic shock model rats was the most significant when the shock was 30min, and all died after complete shock; Rat MAP, to restore it to normal levels, the therapeutic effect is comparable to that of commercially available epinephrine.

Rat MAP significantly decreased after gavage of comparative example 1 anti-shock mixture, and it was difficult to return to normal level, indicating that the role played by ginseng in the whole anti-shock mixture was very important, lacking ginseng, although the therapeutic effect of anti-shock mixture was better than Model group, but the effect is much worse than other groups.

After intragastric administration of comparative example 2 anti-shock mixture, rat MAP decreased more, although better than comparative example 1 and the model group, but worse than other groups, indicating that ginsenoside Rb1 contained in ginseng is the main component for treating hemorrhagic shock, and , Traditional Chinese medicine components pay attention to compatibility. Compared with unpurified ginseng extract, pure ginsenoside Rb1 has a better compatibility effect with other raw material components.

After gavage of comparative example 3 and comparative example 4 anti-shock mixture, rat MAP decreases less, and the effect is better than that of comparative example 1 and comparative example 2, but worse than that of examples 1-3. Both Lingxiaohua and Ruyicao play their respective roles in the whole system, and under the compatibility of the present invention, the raw materials interact with each other, so that the effect of the prepared anti-shock mixture reaches the best.

It should be noted that when a numerical range is involved in the claims of the present invention, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected, because the steps and methods adopted are the same as those in Embodiment 1- 3 Similarly, to avoid redundancy, the present invention describes preferred embodiments, but those skilled in the art can make additional changes and modifications to these embodiments once the basic inventive concept is known. Therefore, it is intended that the appended claims be construed to cover the preferred embodiment as well as all changes and modifications which fall within the scope of the invention.

Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention also intends to include these modifications and variations.

Claims (8)

1. a kind of Hemorrhagic shock mixture containing ginseng, which is characterized in that it prepares raw material and is grouped as by the group of following parts by weight：People Ginseng 20-30 parts, 10-20 parts of paris polyphylla, 10-20 parts of green peel, 5-15 parts of pyrola, 5-15 parts of black nightshade, 5-10 parts of Chinese trumpet creeper, 3-5 parts of wishful grass.

2. the Hemorrhagic shock mixture according to claim 1 containing ginseng, which is characterized in that it prepares raw material by following parts by weight Several groups are grouped as：25 parts of ginseng, 15 parts of paris polyphylla, 15 parts of green peel, 10 parts of pyrola, 10 parts of black nightshade, 5 parts of Chinese trumpet creeper, 5 parts of wishful grass.

3. the preparation method of the Hemorrhagic shock mixture according to claim 1 containing ginseng, which is characterized in that specifically according to following Step is implemented：

Step 1, weigh by weight 20-30 parts of ginseng, 10-20 parts of paris polyphylla, 10-20 parts of green peel, 5-15 parts of pyrola, 5-15 parts of black nightshade, 5-10 parts of Chinese trumpet creeper, 3-5 parts of wishful grass；

Step 2, the ginseng weighed in step 1 is dry, smash it through 20 mesh sieve, obtain ginseng pulverate；By ginseng pulverate and water, wooden Plain peroxidase is according to 100：50：1 mass ratio is uniformly mixed, and is subsequently placed at 60 DEG C and is digested 3h, and enzymolysis finishes to obtain enzyme Solve product；Enzymolysis product is filtered, the first filtrate and filter residue are obtained, into filter residue be added be equivalent to 5 times of filter residue weight ethyl alcohol it is molten Agent ultrasonic extraction filters after extraction, obtains the second filtrate, merges the first filtrate and the second filtrate, is concentrated under reduced pressure into relatively close Degree is 1.3-1.4, obtains ginseng extract；

By ginseng extract and water according to 1g：Silicagel column is crossed after the ratio mixed dissolution of 10mL, the condition for being -0.5Mpa in column pressure Lower carry out silica gel column chromatography, is used in combination the mixed solution of ethyl alcohol and ethyl acetate to be eluted as eluant, eluent, and collection fraction is simultaneously dense Contracting, drying, obtain ginsenoside crude product；

By ginsenoside crude product and n-butanol according to 1g：It is injected into high performance preparative liquid chromatography instrument after the ratio mixed dissolution of 5mL In, it is eluted using ethyl alcohol water mixed solution, collects fraction and concentrate, dry, obtain ginsenoside Rb1；

Step 3, the paris polyphylla weighed in step 1 is dry, smash it through 20 mesh sieve, paris polyphylla powder is obtained, toward seven The microorganism Aspergillus aculeatus culture solution for being equivalent to its weight 5% is added in leaf a flower powder, ferments 5 days, has fermented under the conditions of 28-30 DEG C Finish and obtains tunning；

Tunning and ethanol water are pressed into 1g：The ratio of 5mL mixes, and then flow back at 80 DEG C 3h, mistake after reflux Filter, filtrate be concentrated into relative density be 1.3-1.4 to get Rhizoma Paridis extract；

Step 4, the green peel weighed in step 1, pyrola, black nightshade, Chinese trumpet creeper, wishful grass are mixed, obtains mixture, it is then past The ethyl alcohol for being equivalent to 5 times of mixture quality is added in mixture, is filtered after being heated to reflux 3h, obtains filtrate, filtrate decompression is concentrated Ethyl alcohol is removed, mixed extract is obtained；

Step 5, mixed extract in Rhizoma Paridis extract in ginsenoside Rb1, step 3 in step 2 and step 4 is mixed It closes, is then charged with the sucrose equal with ginsenoside Rb1, Rhizoma Paridis extract and mixed extract total weight Aqueous solution is uniformly mixed to get to the Hemorrhagic shock mixture.

4. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that in the step 2 Ultrasonic extraction conditions are 50 DEG C, power 200w, ultrasonic extraction 30min.

5. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the lignin mistake The enzyme activity of oxide enzyme is 10000U/mL.

6. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the silicagel column Blade diameter length ratio is 1：5.

7. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the microorganism Aspergillus aculeatus Living bacteria count >=10 in culture solution¹⁰CFU/mL。

8. the preparation method of the Hemorrhagic shock mixture according to claim 3 containing ginseng, which is characterized in that the sucrose is water-soluble The mass concentration of liquid is 80%.