CN108315251A - A kind of strain fermentation control system - Google Patents
A kind of strain fermentation control system Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 147
- 230000004151 fermentation Effects 0.000 title claims abstract description 147
- 238000012544 monitoring process Methods 0.000 claims description 132
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 80
- 229910052760 oxygen Inorganic materials 0.000 claims description 80
- 239000001301 oxygen Substances 0.000 claims description 80
- 238000003756 stirring Methods 0.000 claims description 66
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 41
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- 230000000813 microbial effect Effects 0.000 description 1
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Abstract
本发明公开了一种菌种发酵控制系统,涉及生物发酵技术领域,本发明包括发酵罐、控制器和多个发酵参数反馈控制模块,本发明在使用时,各个发酵参数反馈控制模块将对发酵罐内的各个参数进行采集并反馈给控制器,控制器根据得到的参数来控制发酵参数反馈控制模块对发酵罐中的各项参数进行调节,本发明在应用时调节灵活,各参数反馈独立且迅速。
The invention discloses a strain fermentation control system, which relates to the technical field of biological fermentation. The invention includes a fermentation tank, a controller and a plurality of fermentation parameter feedback control modules. When the invention is used, each fermentation parameter feedback control module will control the fermentation Each parameter in the tank is collected and fed back to the controller, and the controller controls the fermentation parameter feedback control module to adjust the parameters in the fermentation tank according to the obtained parameters. The present invention is flexible in application, and the feedback of each parameter is independent and fast.
Description
技术领域technical field
本发明涉及生物发酵技术领域,具体为一种菌种发酵控制系统。The invention relates to the technical field of biological fermentation, in particular to a strain fermentation control system.
背景技术Background technique
微生物发酵生产的水平主要取决于菌种本身的遗传性和培养条件。在一些发酵领域,如菌种发酵,为保证菌种的生长速度和品质,如何优化培养条件对发酵参数进行准确控制是一个研究的重点和热点。现在的自动化发酵控制系统多采用计算机或PLC进行控制。当用计算机控制时,因为现有操作系统的稳定性存在一定的问题,经常会碰到计算机死机的情况,会影响发酵实验的进行,严重时直接导致发酵失败。当用PLC控制时,其各个发酵参数的控制不够精确独立,对PLC编程要求高,且系统复杂。The level of microbial fermentation production mainly depends on the heredity and culture conditions of the strain itself. In some fermentation fields, such as strain fermentation, in order to ensure the growth rate and quality of strains, how to optimize the culture conditions and accurately control the fermentation parameters is a research focus and hotspot. The current automatic fermentation control system is mostly controlled by computer or PLC. When using computer control, because there are certain problems in the stability of the existing operating system, the situation of computer crash is often encountered, which will affect the fermentation experiment, and directly lead to fermentation failure in severe cases. When PLC control is used, the control of each fermentation parameter is not precise and independent, and the requirements for PLC programming are high, and the system is complicated.
发明内容Contents of the invention
本发明目的在于解决上述诸多问题,本发明提供了一种菌种发酵控制系统;其应用时可以及时完成在线数据采集,根据所采集的数据进行在线实时控制。The purpose of the present invention is to solve the above problems. The present invention provides a strain fermentation control system; when it is applied, online data collection can be completed in time, and online real-time control can be performed according to the collected data.
本发明的目的主要通过以下技术方案实现:The purpose of the present invention is mainly achieved through the following technical solutions:
一种菌种发酵控制系统,包括发酵罐,还包括控制器和多个发酵参数监控模块,所述控制器用于控制多个所述发酵参数监控模块来调整发酵罐中的发酵参数。A strain fermentation control system includes a fermenter, and also includes a controller and a plurality of fermentation parameter monitoring modules, and the controller is used to control the plurality of fermentation parameter monitoring modules to adjust the fermentation parameters in the fermenter.
进一步地,所述控制器通过RS485总线与发酵参数监控模块通讯来控制发酵罐中的发酵参数,所述发酵参数监控模块包括温度监控模块、PH监控模块、溶氧监控模块、搅拌速度监控模块、泡沫监控模块、补料控制模块、空气流量监控模块和罐压监控模块;Further, the controller communicates with the fermentation parameter monitoring module through the RS485 bus to control the fermentation parameters in the fermenter, and the fermentation parameter monitoring module includes a temperature monitoring module, a pH monitoring module, a dissolved oxygen monitoring module, a stirring speed monitoring module, Foam monitoring module, feeding control module, air flow monitoring module and tank pressure monitoring module;
所述温度监控模块用于采集发酵罐中的温度数据并反馈给控制器,同时温度监控模块能够控制发酵罐中的温度;若温度数据符合发酵要求,则控制器不做指示;若温度数据不符合发酵要求,则控制器控制温度监控模块来改变发酵罐中的温度;The temperature monitoring module is used to collect temperature data in the fermenter and feed it back to the controller, and the temperature monitoring module can control the temperature in the fermenter; if the temperature data meets the fermentation requirements, the controller does not give instructions; if the temperature data does not If the fermentation requirements are met, the controller controls the temperature monitoring module to change the temperature in the fermenter;
所述PH监控模块用于采集发酵罐中的PH值并反馈给控制器,同时PH监控模块能够控制发酵罐中的PH值;若PH值符合发酵要求,则控制器不做指示;若PH值不符合发酵要求,则控制器控制PH监控模块来改变发酵罐中的PH值;The pH monitoring module is used to collect the pH value in the fermenter and feed it back to the controller, and the pH monitoring module can control the pH value in the fermenter; if the pH value meets the fermentation requirements, the controller will not give instructions; if the pH value If the fermentation requirements are not met, the controller controls the pH monitoring module to change the pH value in the fermenter;
所述溶氧监控模块用于采集发酵罐中的溶氧值并反馈给控制器,同时溶氧监控模块能够控制发酵罐中的溶氧值;若溶氧值符合发酵要求,则控制器不做指示;若溶氧值不符合发酵要求,则控制器控制溶氧监控模块来改变发酵罐中的溶氧值;The dissolved oxygen monitoring module is used to collect the dissolved oxygen value in the fermenter and feed it back to the controller. At the same time, the dissolved oxygen monitoring module can control the dissolved oxygen value in the fermenter; if the dissolved oxygen value meets the fermentation requirements, the controller does not indication; if the dissolved oxygen value does not meet the fermentation requirements, the controller controls the dissolved oxygen monitoring module to change the dissolved oxygen value in the fermenter;
所述搅拌速度监控模块用于采集发酵罐中的搅拌速度数据并反馈给控制器,同时搅拌速度监控模块能够控制发酵罐中的搅拌速度;若搅拌速度数据符合发酵要求,则控制器不做指示;若搅拌速度数据不符合发酵要求,则控制器控制搅拌速度监控模块来改变发酵罐中的搅拌速度;The stirring speed monitoring module is used to collect the stirring speed data in the fermenter and feed it back to the controller, and the stirring speed monitoring module can control the stirring speed in the fermenter; if the stirring speed data meets the fermentation requirements, the controller will not give instructions ; If the stirring speed data does not meet the fermentation requirements, the controller controls the stirring speed monitoring module to change the stirring speed in the fermenter;
所述泡沫监控模块用于采集发酵罐中的泡沫量并反馈给控制器,同时泡沫监控模块能够控制发酵罐中的泡沫量;若泡沫量符合发酵要求,则控制器不做指示;若泡沫量不符合发酵要求,则控制器控制泡沫监控模块来改变发酵罐中的泡沫量;The foam monitoring module is used to collect the amount of foam in the fermenter and feed it back to the controller, and the foam monitoring module can control the amount of foam in the fermenter; if the amount of foam meets the fermentation requirements, the controller will not give instructions; if the amount of foam If the fermentation requirements are not met, the controller controls the foam monitoring module to change the amount of foam in the fermenter;
所述补料控制模块用于为发酵罐中进行补料,控制器控制补料控制模块来向发酵罐内补料;The feed control module is used to feed the fermenter, and the controller controls the feed control module to feed the fermenter;
所述空气流量监控模块用于监控发酵过程中的空气流量并反馈给控制器,其能够控制发酵罐的溶氧量;The air flow monitoring module is used to monitor the air flow in the fermentation process and feed back to the controller, which can control the dissolved oxygen of the fermenter;
所述罐压监控模块用于采集发酵罐中的压强值并反馈给控制器,同时罐压监控模块能够控制发酵罐中的压强值;若压强值符合发酵要求,则控制器不做指示;若压强值不符合发酵要求,则控制器控制罐压监控模块来改变发酵罐中的压强值。The tank pressure monitoring module is used to collect the pressure value in the fermentation tank and feed it back to the controller, and the tank pressure monitoring module can control the pressure value in the fermentation tank; if the pressure value meets the fermentation requirements, the controller will not give instructions; if If the pressure value does not meet the fermentation requirements, the controller controls the tank pressure monitoring module to change the pressure value in the fermentation tank.
进一步地,所述温度监控模块包括温度探头、热水箱、冷水箱、电加热棒和两个温控水泵,电加热棒位于热水箱内,用于加热产生热水;一个温控水泵的两端分别与热水箱和发酵罐连接,另一个温控水泵的两端分别与冷水箱和发酵罐连接;所述温度探头伸入发酵罐内用于采集发酵罐内的温度数据,温度数据通过RS485总线通讯发送给控制器,所述控制器根据温度数据来控制两个温控水泵的启停从而控制发酵罐中的温度。Further, the temperature monitoring module includes a temperature probe, a hot water tank, a cold water tank, an electric heating rod and two temperature-controlled water pumps, and the electric heating rod is located in the hot water tank for heating to generate hot water; one temperature-controlled water pump The two ends are respectively connected to the hot water tank and the fermentation tank, and the two ends of the other temperature-controlled water pump are respectively connected to the cold water tank and the fermentation tank; Send it to the controller through RS485 bus communication, and the controller controls the start and stop of two temperature-controlled water pumps according to the temperature data to control the temperature in the fermenter.
进一步地,所述PH监控模块包括PH探头、碱液罐、酸液罐、加酸蠕动泵和加碱蠕动泵,其中加碱蠕动泵的两端分别与碱液罐和发酵罐连接,加酸蠕动泵的两端分别与酸液罐和发酵罐连接,所述PH探头伸入发酵罐内用于采集发酵罐内的PH值,PH值通过RS485总线通讯发送给控制器,所述控制器根据接收到的PH值来控制加酸、加碱蠕动泵的启停从而控制发酵罐中的PH值。Further, the pH monitoring module includes a pH probe, a lye tank, an acid tank, an acid-adding peristaltic pump and an alkali-adding peristaltic pump, wherein the two ends of the alkali-adding peristaltic pump are respectively connected with the lye tank and the fermenter, The two ends of the peristaltic pump are respectively connected with the acid tank and the fermentation tank, and the pH probe is inserted into the fermentation tank to collect the pH value in the fermentation tank, and the pH value is sent to the controller through the RS485 bus communication, and the controller according to The received pH value is used to control the start and stop of the peristaltic pump for adding acid and alkali so as to control the pH value in the fermenter.
进一步地,所述溶氧监控模块包括溶氧信号变送器和溶氧探头,所述溶氧探头将其检测到的信号发送给溶氧信号变送器,溶氧信号变送器将溶氧探头发送的信号转化为可读取的实际溶氧值,溶氧信号变送器通过RS485总线通讯将溶氧值发送给控制器,控制器根据溶氧值来给空气流量监控模块和搅拌速度监控模块发送控制指令从而控制发酵罐中的溶氧值。Further, the dissolved oxygen monitoring module includes a dissolved oxygen signal transmitter and a dissolved oxygen probe, the dissolved oxygen probe sends the detected signal to the dissolved oxygen signal transmitter, and the dissolved oxygen signal transmitter sends the dissolved oxygen The signal sent by the probe is converted into the actual dissolved oxygen value that can be read. The dissolved oxygen signal transmitter sends the dissolved oxygen value to the controller through RS485 bus communication. The controller sends the air flow monitoring module and stirring speed monitoring according to the dissolved oxygen value The module sends control commands to control the dissolved oxygen value in the fermenter.
进一步地,所述搅拌速度监控模块包括变频器和搅拌电机,搅拌电机的输出端上设有搅拌桨,所述搅拌电机的搅拌桨位于发酵罐内部,变频器与控制器通过RS485总线通讯,所述变频器与搅拌电机连接,所述控制器给变频器发送控制指令从而控制搅拌电机的搅拌速度。Further, the stirring speed monitoring module includes a frequency converter and a stirring motor, the output end of the stirring motor is provided with a stirring paddle, the stirring paddle of the stirring motor is located inside the fermenter, and the frequency converter communicates with the controller through the RS485 bus. The frequency converter is connected to the stirring motor, and the controller sends control instructions to the frequency converter to control the stirring speed of the stirring motor.
进一步地,所述泡沫监控模块包括泡沫探头、消泡蠕动泵和消泡液罐,所述泡沫探头从发酵罐的顶部插入发酵罐内,所述消泡蠕动泵两端分别连接发酵罐和消泡液罐,消泡液罐内充有消泡剂,所述控制器根据泡沫探头检测的信号来控制消泡蠕动泵的启停;泡沫探头探测到发酵罐内有过量泡沫时控制器控制消泡蠕动泵启动,消泡蠕动泵将消泡液罐中的消泡剂泵入发酵罐从而进行消泡。Further, the foam monitoring module includes a foam probe, a defoaming peristaltic pump and a defoaming liquid tank, the foam probe is inserted into the fermenter from the top of the fermenter, and the two ends of the defoaming peristaltic pump are respectively connected to the fermenter and the defoaming tank. Foam liquid tank, defoamer is filled in the defoamer liquid tank, and described controller controls the start and stop of defoaming peristaltic pump according to the signal detected by foam probe; The foam peristaltic pump starts, and the defoaming peristaltic pump pumps the defoamer in the defoaming liquid tank into the fermenter for defoaming.
进一步地,所述补料控制模块包括补料罐和补料蠕动泵,补料蠕动泵的两端分别于补料罐和发酵罐连接,所述控制器根据发酵要求控制补料蠕动泵的启停来对发酵罐进行补料。Further, the feeding control module includes a feeding tank and a feeding peristaltic pump, the two ends of the feeding peristaltic pump are respectively connected to the feeding tank and the fermenter, and the controller controls the starting of the feeding peristaltic pump according to fermentation requirements. Stop to refill the fermenter.
进一步地,所述空气流量监控模块包括空气流量计和进气阀门,进气阀门位于发酵罐外,所述空气流量计两端分别连接进气阀门和发酵罐,空气流量计与控制器通过RS485总线通讯,所述控制器根据溶氧值来控制进气阀门开启度从而控制通入空气流量的大小。Further, the air flow monitoring module includes an air flow meter and an air intake valve, the air intake valve is located outside the fermenter, the two ends of the air flow meter are respectively connected to the air intake valve and the fermenter, and the air flow meter and the controller are connected via RS485 Bus communication, the controller controls the opening degree of the intake valve according to the dissolved oxygen value so as to control the flow rate of the incoming air.
进一步地,所述罐压监控模块包括罐压测量传感器和排气阀门,所述罐压测量传感器的探测端伸入发酵罐,罐压测量传感器与控制器通过RS485总线通讯,罐压测量传感器将发酵罐内的压强值反馈给控制器,所述控制器根据压强值来控制排气阀门的开闭从而控制发酵罐内的压强值。Further, the tank pressure monitoring module includes a tank pressure measurement sensor and an exhaust valve, the detection end of the tank pressure measurement sensor extends into the fermenter, the tank pressure measurement sensor communicates with the controller through the RS485 bus, and the tank pressure measurement sensor will The pressure value in the fermentation tank is fed back to the controller, and the controller controls the opening and closing of the exhaust valve according to the pressure value so as to control the pressure value in the fermentation tank.
通过使用本发明,可以产生以下有益效果:本发明包括发酵罐、控制器和多个发酵参数监控模块,本发明在使用时,各个发酵参数监控模块将对发酵罐内的各个参数进行监控并反馈给控制器,控制器根据得到的测量值来向各个发酵参数监控模块发出控制指令从而实现对发酵罐中的各项参数的控制。本发明在应用时发酵参数可通过相应的发酵参数监控模块进行灵活的控制,并且各发酵参数还实时的反馈给控制器,从而实现控制器对整个发酵过程的监控,进而保证发酵过程准确、可靠的完成。By using the present invention, the following beneficial effects can be produced: the present invention includes a fermenter, a controller and a plurality of fermentation parameter monitoring modules. When the present invention is in use, each fermentation parameter monitoring module will monitor and feed back each parameter in the fermenter to the controller, and the controller sends control instructions to each fermentation parameter monitoring module according to the obtained measured values so as to realize the control of various parameters in the fermenter. When the present invention is applied, the fermentation parameters can be flexibly controlled through the corresponding fermentation parameter monitoring module, and each fermentation parameter is also fed back to the controller in real time, so as to realize the monitoring of the entire fermentation process by the controller, thereby ensuring the accuracy and reliability of the fermentation process the completion.
附图说明Description of drawings
此处所说明的附图用来提供对本发明实施例的进一步理解,构成本申请的一部分,并不构成对本发明实施例的限定。在附图中:The drawings described here are used to provide a further understanding of the embodiments of the present invention, constitute a part of the application, and do not limit the embodiments of the present invention. In the attached picture:
图1为本发明的系统结构图。Fig. 1 is a system structure diagram of the present invention.
具体实施方式Detailed ways
为使本发明的目的、技术方案和优点更加清楚明白,下面结合实施例和附图,对本发明作进一步的详细说明,本发明的示意性实施方式及其说明仅用于解释本发明,并不作为对本发明的限定。In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples and accompanying drawings. As a limitation of the present invention.
如图1所示,一种菌种发酵控制系统,包括发酵罐,还包括控制器和各种发酵参数监控模块,控制器用于控制发酵参数监控模块来调整发酵罐中的发酵参数;优选的,控制器通过RS485总线与各发酵参数监控模块连接来控制发酵罐中的发酵参数;通讯协议采用MODBUS-RTU;发酵参数监控模块包括温度监控模块、PH监控模块、溶氧监控模块、搅拌速度监控模块、泡沫监控模块、补料控制模块、空气流量监控模块和罐压监控模块。As shown in Figure 1, a kind of strain fermentation control system, comprises fermentor, also comprises controller and various fermentation parameter monitoring modules, and controller is used for controlling the fermentation parameter monitoring module to adjust the fermentation parameter in fermenter; Preferably, The controller is connected to each fermentation parameter monitoring module through the RS485 bus to control the fermentation parameters in the fermentation tank; the communication protocol adopts MODBUS-RTU; the fermentation parameter monitoring module includes a temperature monitoring module, a PH monitoring module, a dissolved oxygen monitoring module, and a stirring speed monitoring module , foam monitoring module, feeding control module, air flow monitoring module and tank pressure monitoring module.
具体的,控制器通过RS485总线与发酵参数监控模块通讯来控制发酵罐中的发酵参数;Specifically, the controller communicates with the fermentation parameter monitoring module through the RS485 bus to control the fermentation parameters in the fermentation tank;
温度监控模块用于采集发酵罐中的温度数据并反馈给控制器,同时温度监控模块能够控制发酵罐中的温度;若温度数据符合发酵要求,则控制器不做指示;若温度数据不符合发酵要求,则控制器控制温度监控模块来改变发酵罐中的温度;相应的,温度监控模块包括温度探头、热水箱、冷水箱、电加热棒和两个温控水泵,电加热棒位于热水箱内,用于加热产生热水;一个温控水泵的两端分别与热水箱和发酵罐连接,另一个温控水泵的两端分别与冷水箱和发酵罐连接;温度探头伸入发酵罐内用于采集发酵罐内的温度数据,温度数据通过RS485总线通讯发送给控制器,控制器根据温度数据来控制两个温控水泵的启停从而控制发酵罐中的温度。The temperature monitoring module is used to collect the temperature data in the fermentation tank and feed it back to the controller. At the same time, the temperature monitoring module can control the temperature in the fermentation tank; if the temperature data meets the fermentation requirements, the controller will not give instructions; if the temperature data does not meet the fermentation requirements If required, the controller controls the temperature monitoring module to change the temperature in the fermenter; correspondingly, the temperature monitoring module includes a temperature probe, a hot water tank, a cold water tank, an electric heating rod and two temperature-controlled water pumps, and the electric heating rod is located in the hot water tank. Inside the tank, it is used for heating to generate hot water; the two ends of one temperature-controlled water pump are respectively connected with the hot water tank and the fermentation tank, and the two ends of the other temperature-controlled water pump are respectively connected with the cold water tank and the fermentation tank; the temperature probe extends into the fermentation tank It is used to collect the temperature data in the fermenter. The temperature data is sent to the controller through RS485 bus communication. The controller controls the start and stop of the two temperature-controlled water pumps according to the temperature data to control the temperature in the fermenter.
PH监控模块用于采集发酵罐中的PH值并反馈给控制器,同时PH监控模块能够控制发酵罐中的PH值;若PH值符合发酵要求,则控制器不做指示;若PH值不符合发酵要求,则控制器控制PH监控模块来改变发酵罐中的PH值;其中,PH监控模块包括PH探头、碱液罐、酸液罐、加酸蠕动泵和加碱蠕动泵,其中加碱蠕动泵的两端分别与碱液罐和发酵罐连接,加酸蠕动泵的两端分别与酸液罐和发酵罐连接,PH探头伸入发酵罐内用于采集发酵罐内的PH值,PH探头采用INPRO3030/120,PH探头再将PH值发送给PH信号变送器,PH信号变送器采用pH2050e变送器;PH信号变送器将PH值通过RS485总线通讯发送给控制器,控制器根据接收到的PH值来控制加酸、加碱蠕动泵的启停从而控制发酵罐中的PH值。The PH monitoring module is used to collect the PH value in the fermenter and feed it back to the controller. At the same time, the PH monitoring module can control the PH value in the fermenter; if the PH value meets the fermentation requirements, the controller will not give instructions; if the PH value does not meet the requirements Fermentation requirements, then the controller controls the pH monitoring module to change the pH value in the fermenter; wherein, the pH monitoring module includes a pH probe, an alkali tank, an acid tank, an acid peristaltic pump and an alkali peristaltic pump, wherein the alkali peristaltic The two ends of the pump are respectively connected with the lye tank and the fermentation tank, and the two ends of the acid-adding peristaltic pump are respectively connected with the acid tank and the fermentation tank. The pH probe is inserted into the fermentation tank to collect the pH value in the fermentation tank. Using INPRO3030/120, the pH probe sends the pH value to the pH signal transmitter, and the pH signal transmitter adopts the pH2050e transmitter; the pH signal transmitter sends the pH value to the controller through RS485 bus communication, and the controller according to The received pH value is used to control the start and stop of the peristaltic pump for adding acid and alkali so as to control the pH value in the fermenter.
溶氧监控模块用于采集发酵罐中的溶氧值并反馈给控制器,同时溶氧监控模块能够控制发酵罐中的溶氧值;若溶氧值符合发酵要求,则控制器不做指示;若溶氧值不符合发酵要求,则控制器控制溶氧监控模块来改变发酵罐中的溶氧值;溶氧监控模块包括溶氧信号变送器和溶氧探头,溶氧探头将其检测到的信号发送给溶氧信号变送器,溶氧信号变送器将溶氧探头发送的信号转化为可读取的实际溶氧值,溶氧信号变送器通过RS485总线通讯将溶氧值发送给控制器,控制器根据溶氧值来给空气流量监控模块和搅拌速度监控模块发送控制指令从而控制发酵罐中的溶氧值;具体的,这里溶氧探头采用INPRO6800/120,溶氧信号变送器采用DO4050e变送器The dissolved oxygen monitoring module is used to collect the dissolved oxygen value in the fermenter and feed it back to the controller. At the same time, the dissolved oxygen monitoring module can control the dissolved oxygen value in the fermenter; if the dissolved oxygen value meets the fermentation requirements, the controller will not give instructions; If the dissolved oxygen value does not meet the fermentation requirements, the controller controls the dissolved oxygen monitoring module to change the dissolved oxygen value in the fermenter; the dissolved oxygen monitoring module includes a dissolved oxygen signal transmitter and a dissolved oxygen probe, and the dissolved oxygen probe detects it. The signal is sent to the dissolved oxygen signal transmitter, the dissolved oxygen signal transmitter converts the signal sent by the dissolved oxygen probe into the actual dissolved oxygen value that can be read, and the dissolved oxygen signal transmitter sends the dissolved oxygen value through RS485 bus communication To the controller, the controller sends control instructions to the air flow monitoring module and stirring speed monitoring module according to the dissolved oxygen value to control the dissolved oxygen value in the fermenter; specifically, the dissolved oxygen probe here uses INPRO6800/120, and the dissolved oxygen signal changes Transmitter adopts DO4050e transmitter
搅拌速度监控模块用于采集发酵罐中的搅拌速度数据并反馈给控制器,同时搅拌速度监控模块能够控制发酵罐中的搅拌速度;若搅拌速度数据符合发酵要求,则控制器不做指示;若搅拌速度数据不符合发酵要求,则控制器控制搅拌速度监控模块来改变发酵罐中的搅拌速度;搅拌速度监控模块包括变频器和搅拌电机,这里变频器采用三环SH-HVF II10K/600型;搅拌电机的输出端上设有搅拌桨,搅拌电机的搅拌桨位于发酵罐内部,变频器与控制器通过RS485总线通讯,变频器与搅拌电机连接,控制器给变频器发送控制指令从而控制搅拌电机的搅拌速度。The stirring speed monitoring module is used to collect the stirring speed data in the fermenter and feed it back to the controller. At the same time, the stirring speed monitoring module can control the stirring speed in the fermenter; if the stirring speed data meets the fermentation requirements, the controller will not give instructions; if If the stirring speed data does not meet the fermentation requirements, the controller controls the stirring speed monitoring module to change the stirring speed in the fermenter; the stirring speed monitoring module includes a frequency converter and a stirring motor, and the frequency converter here uses a three-ring SH-HVF II10K/600 type; The output end of the stirring motor is equipped with a stirring paddle, the stirring paddle of the stirring motor is located inside the fermentation tank, the frequency converter communicates with the controller through the RS485 bus, the frequency converter is connected with the stirring motor, and the controller sends control commands to the frequency converter to control the stirring motor stirring speed.
泡沫监控模块用于采集发酵罐中的泡沫量并反馈给控制器,同时泡沫监控模块能够控制发酵罐中的泡沫量;若泡沫量符合发酵要求,则控制器不做指示;若泡沫量不符合发酵要求,则控制器控制泡沫监控模块来改变发酵罐中的泡沫量;泡沫监控模块包括泡沫探头、消泡蠕动泵和消泡液罐,泡沫探头从发酵罐的顶部插入发酵罐内,消泡蠕动泵两端分别连接发酵罐和消泡液罐,消泡液罐内充有消泡剂,控制器根据泡沫探头检测的信号来控制消泡蠕动泵的启停;泡沫探头探测到发酵罐内有过量泡沫时控制器控制消泡蠕动泵启动,消泡蠕动泵将消泡液罐中的消泡剂泵入发酵罐从而进行消泡。控制器一般通过电平信号来控制消泡蠕动泵的启停,泡沫探头所发出信号的一般状态若为高电平信号,当检测到泡沫时泡沫探头的电平信号转化为低电平,低电平信号发送给控制器后,控制器将启动消泡蠕动泵,上述工作原理及方式是泡沫探头内置的工作原理。The foam monitoring module is used to collect the amount of foam in the fermenter and feed it back to the controller. At the same time, the foam monitoring module can control the amount of foam in the fermenter; if the amount of foam meets the fermentation requirements, the controller will not give instructions; if the amount of foam does not meet the requirements Fermentation requirements, then the controller controls the foam monitoring module to change the amount of foam in the fermenter; the foam monitoring module includes a foam probe, a defoaming peristaltic pump and a defoaming liquid tank, the foam probe is inserted into the fermenter from the top of the fermenter, The two ends of the peristaltic pump are respectively connected to the fermentation tank and the defoaming liquid tank. The defoaming liquid tank is filled with defoaming agent. The controller controls the start and stop of the defoaming peristaltic pump according to the signal detected by the foam probe; When there is excessive foam, the controller controls the defoaming peristaltic pump to start, and the defoaming peristaltic pump pumps the defoaming agent in the defoaming liquid tank into the fermenter for defoaming. The controller generally controls the start and stop of the defoaming peristaltic pump through the level signal. If the general state of the signal sent by the foam probe is a high level signal, when the foam is detected, the level signal of the foam probe is converted into a low level, and the low level signal is low. After the level signal is sent to the controller, the controller will start the defoaming peristaltic pump. The above working principle and method are the working principles built into the foam probe.
补料控制模块用于为发酵罐中进行补料,控制器控制补料控制模块来向发酵罐内补料;补料控制模块包括补料罐和补料蠕动泵,补料蠕动泵的两端分别于补料罐和发酵罐连接,控制器根据发酵要求控制补料蠕动泵的启停来对发酵罐进行补料。发酵前补料的控制参数需要在控制器中设定好,发酵开始后,控制器根据设定的参数在合适的时间段通过启动补料蠕动泵对发酵罐进行补料。The feeding control module is used to feed the fermenter, and the controller controls the feeding control module to feed the fermenter; the feeding control module includes a feeding tank and a feeding peristaltic pump, and the two ends of the feeding peristaltic pump The feeding tank and the fermentation tank are respectively connected, and the controller controls the start and stop of the feeding peristaltic pump to feed the fermentation tank according to the fermentation requirements. The control parameters for feeding before fermentation need to be set in the controller. After the fermentation starts, the controller will feed the fermenter by starting the feeding peristaltic pump at an appropriate time according to the set parameters.
空气流量监控模块用于监控发酵过程中的空气流量并反馈给控制器,其能够控制发酵罐的溶氧量;空气流量监控模块包括空气流量计和进气阀门,这里空气流量控制器的型号是D07-7M/ZM(0-6SLM);进气阀门位于发酵罐外,空气流量计两端分别连接进气阀门和发酵罐,空气流量计与控制器通过RS485总线通讯,控制器根据溶氧值来控制进气阀门开启度从而控制通入空气流量的大小;空气流量的大小影响发酵过程中的溶氧量,若溶氧的测量值小于设定值,空气流量监控模块将控制空气流量的增加从而使提高发酵液中的溶氧量。The air flow monitoring module is used to monitor the air flow during the fermentation process and feed it back to the controller, which can control the dissolved oxygen in the fermenter; the air flow monitoring module includes an air flow meter and an intake valve, and the model of the air flow controller here is D07-7M/ZM (0-6SLM); the air intake valve is located outside the fermentation tank, the two ends of the air flow meter are respectively connected to the intake valve and the fermentation tank, the air flow meter and the controller communicate through the RS485 bus, and the controller according to the dissolved oxygen value To control the opening of the intake valve to control the size of the air flow; the size of the air flow affects the amount of dissolved oxygen in the fermentation process. If the measured value of dissolved oxygen is less than the set value, the air flow monitoring module will control the increase of the air flow Thereby increasing the dissolved oxygen in the fermentation broth.
罐压监控模块用于采集发酵罐中的压强值并反馈给控制器,同时罐压监控模块能够控制发酵罐中的压强值;若压强值符合发酵要求,则控制器不做指示;若压强值不符合发酵要求,则控制器控制罐压监控模块来改变发酵罐中的压强值;罐压监控模块包括罐压测量传感器和排气阀门,罐压测量传感器的探测端伸入发酵罐,罐压测量传感器与控制器通过RS485总线通讯,罐压测量传感器将发酵罐内的压强值反馈给控制器,这里罐压测量传感器的型号是PMC534-11EE2P6(T),控制器根据压强值来控制排气阀门的开闭从而控制发酵罐内的压强值。The tank pressure monitoring module is used to collect the pressure value in the fermentation tank and feed it back to the controller. At the same time, the tank pressure monitoring module can control the pressure value in the fermentation tank; if the pressure value meets the fermentation requirements, the controller will not give instructions; if the pressure value If the fermentation requirements are not met, the controller controls the tank pressure monitoring module to change the pressure value in the fermentation tank; the tank pressure monitoring module includes a tank pressure measurement sensor and an exhaust valve, the detection end of the tank pressure measurement sensor extends into the fermentation tank, and the tank pressure The measurement sensor communicates with the controller through the RS485 bus. The tank pressure measurement sensor feeds back the pressure value in the fermentation tank to the controller. The model of the tank pressure measurement sensor here is PMC534-11EE2P6(T), and the controller controls the exhaust according to the pressure value. The opening and closing of the valve controls the pressure value in the fermenter.
在一种优选实施例中:In a preferred embodiment:
还包括上位机,控制器的控制芯片采用STM32,上位机可以选用计算机来完成人机交互,上位机将控制器的各个参数采集显示,并将需要设定的参数通过上位机传给控制器。在温度监控模块中,温控水泵与电磁阀共同作用控制升、降温,热水箱里采用电加热的方式来对水体进行加热,罐温控制状态分自控或手控,罐温自控的控制方式为PID定值控制或PID时序控制,罐温手控时可直接手动进行升温或降温控制。可通过计算机上参数设定功能项进行设定罐温(升温)控制的设定值(控制降温控制的设定值自动跟随变化)、上限偏差报警值和上限报警值。需要罐温电加热升温控制时,设定罐温(升温)控制的设定值,将罐温升温控制状态置于自动的位置。当罐温测量值低于罐温设定值时,自动开启热水循环水泵进行罐温的升温控制。需要罐温降温控制时,将罐温降温控制状态置于自动的位置。当罐温测量值大于罐温设定值时,间隙式开启电磁阀和冷水循环水泵进行罐温降温控制。发酵需要使用恒温水升温时,恒温水水温的设定值应设为大于罐温设定值+2℃~+5℃左右。需要使用恒温水升温时,将恒温水温控制状态置于自动功能状态。当恒温水水温的测量值小于设定值时自动进行恒温水电加热升温控制动作。It also includes the upper computer, the control chip of the controller adopts STM32, the upper computer can use a computer to complete the human-computer interaction, the upper computer collects and displays each parameter of the controller, and transmits the parameters that need to be set to the controller through the upper computer. In the temperature monitoring module, the temperature-controlled water pump and the solenoid valve work together to control the temperature rise and fall. The electric heating method is used to heat the water body in the hot water tank. The tank temperature control state is divided into automatic control or manual control, and the tank temperature automatic control control mode It is PID fixed value control or PID sequence control. When the tank temperature is manually controlled, it can be directly controlled by manual temperature rise or fall. The set value of the tank temperature (temperature rise) control (the set value of the temperature control control will automatically follow the change), the upper limit deviation alarm value and the upper limit alarm value can be set through the parameter setting function item on the computer. When the tank temperature electric heating temperature rise control is required, set the set value of the tank temperature (temperature rise) control, and set the tank temperature rise control state to the automatic position. When the measured value of the tank temperature is lower than the set value of the tank temperature, the hot water circulating pump is automatically turned on to control the temperature rise of the tank. When tank temperature cooling control is required, set the tank temperature cooling control state to the automatic position. When the measured value of the tank temperature is greater than the set value of the tank temperature, the electromagnetic valve and the cold water circulating water pump are intermittently opened to control the temperature of the tank. When fermentation needs to use constant temperature water to raise the temperature, the set value of the constant temperature water temperature should be set to be about +2°C~+5°C higher than the set value of the tank temperature. When it is necessary to use constant temperature water to raise the temperature, set the constant temperature water temperature control state to the automatic function state. When the measured value of the constant temperature water temperature is lower than the set value, the constant temperature water electric heating temperature rise control action is automatically performed.
在搅拌速度监控模块中,搅拌速度通过STM32从控制器输出控制信号到变频器,然后通过变频器控制搅拌电机。可通过计算机上参数设定功能项进行设定搅拌速度控制的设定值、上限偏差报警值和上限报警值。当需要搅拌速度控制时,设定搅拌速度控制的设定值,然后开启此功能。另外,注意搅拌电机不充许频繁起停,否则可能出现因电流超负荷而不能正常运转。In the stirring speed monitoring module, the stirring speed outputs the control signal from the controller to the frequency converter through STM32, and then controls the stirring motor through the frequency converter. The setting value of the stirring speed control, the upper limit deviation alarm value and the upper limit alarm value can be set through the parameter setting function item on the computer. When stirring speed control is required, set the setting value of stirring speed control, and then turn on this function. In addition, note that the stirring motor is not allowed to start and stop frequently, otherwise it may not work properly due to current overload.
在溶氧监测模块中,溶氧控制通过STM32从控制器输出控制指令到变频器或进气阀门,采用调整搅拌速度或进气流量进行控制。将溶氧设定值设为需要的值,然后开启此功能,此时搅拌速度(或进气流量)自动根据溶氧的变化而调整。溶氧报警方式为上下限绝对值报警或不报警,可通过计算机上参数设定功能项进行设定溶氧控制的设定值、上限偏差报警值和下限偏差报警值设定。In the dissolved oxygen monitoring module, the dissolved oxygen control is controlled by outputting control instructions from the controller to the frequency converter or the intake valve through STM32, and controlling by adjusting the stirring speed or the intake flow. Set the dissolved oxygen setting value to the required value, and then turn on this function. At this time, the stirring speed (or intake flow) will be automatically adjusted according to the change of dissolved oxygen. The dissolved oxygen alarm mode is upper and lower limit absolute value alarm or no alarm, and the set value of dissolved oxygen control, upper limit deviation alarm value and lower limit deviation alarm value can be set through the parameter setting function item on the computer.
在PH监控模块中,PH控制通过STM32从控制器输出控制信号到加酸、加碱蠕动泵进行加酸或加碱控制。可通过计算机上参数设定功能项进行PH控制的设定值、上限偏差报警值和下限偏差报警值设定。PH控制可以进行自控或手控,PH自控的控制方式为PID定值控制或PID时序控制。当PH测量值低于PH设定值时间隙加碱;当PH测量值高于PH设定值时间隙加酸。In the PH monitoring module, the PH control uses the STM32 to output the control signal from the controller to the acid and base peristaltic pump for acid or base control. The setting value of PH control, the upper limit deviation alarm value and the lower limit deviation alarm value can be set through the parameter setting function item on the computer. PH control can be controlled automatically or manually, and the control mode of PH automatic control is PID fixed value control or PID sequence control. When the pH measurement value is lower than the pH setting value, the base is added intermittently; when the pH measurement value is higher than the pH setting value, the acid is added intermittently.
补料控制通过STM32从控制器直接输出控制信号到补料蠕动泵完成。补料控制有自控或手控功能,补料自控时控制方式为开关量固定时间比例控制或开关量时序时间比例控制;补料手控时可直接手动开/关补料蠕动泵。The feeding control is completed by directly outputting the control signal from the controller to the feeding peristaltic pump through STM32. Feeding control has automatic control or manual control function. The control mode of feeding automatic control is fixed time proportional control of switching value or time sequence time proportional control of switching value; manual feeding can be directly turned on/off the feeding peristaltic pump.
泡沫监控模块中,消泡动作通过STM32从控制器直接输出控制信号到消泡蠕动泵完成。消泡控制状态有自控或手控功能:消泡自控时控制方式为开关量固定时间比例控制或开关量时序时间比例控制;消泡手控时可直接手动开、关消泡蠕动泵。有过量泡沫时,系统也会给出泡沫报警提示信号。In the foam monitoring module, the defoaming action is completed by directly outputting control signals from the controller to the defoaming peristaltic pump through STM32. The defoaming control state has automatic control or manual control function: when the defoaming is automatically controlled, the control method is the fixed time proportional control of the switch or the time sequence time proportional control of the switch; when the defoaming is manually controlled, the defoaming peristaltic pump can be turned on and off manually. When there is excessive foam, the system will also give a foam alarm signal.
在空气流量监控模块中,空气流量的控制通过STM32从控制器输出控制信号到进气阀门,从而调节进气阀的开启度来完成。空气流量自动的控制方式为模拟量PID定值控制或模拟量PID时序控制,报警方式为上下限偏差报警或不报警。参数设定可通过计算机上参数设定功能项进行设定流量控制的设定值、上限偏差报警值和下限偏差报警值。自动控制时:1)打开进气调节阀的截止阀,并关闭旁路阀;2)几分钟后空气流量可自动控制在设定值附近。In the air flow monitoring module, the control of the air flow is accomplished by outputting a control signal from the controller to the intake valve through the STM32, thereby adjusting the opening degree of the intake valve. The air flow automatic control mode is analog PID fixed value control or analog PID sequential control, and the alarm mode is upper and lower limit deviation alarm or no alarm. Parameter setting can set the set value of flow control, the upper limit deviation alarm value and the lower limit deviation alarm value through the parameter setting function item on the computer. During automatic control: 1) Open the cut-off valve of the intake regulating valve and close the bypass valve; 2) After a few minutes, the air flow can be automatically controlled near the set value.
在罐压监控模块中,罐压自控的控制方式为模拟量PID定值控制或模拟量PID时序控制,报警方式为上下限偏差报警或不报警。参数设定:可通过计算机上参数设定功能项进行设定流量控制的设定值、上限偏差报警值和下限偏差报警值。自动控制时:1)打开排气调节阀的截止阀,并关闭旁路阀;2)几分钟后罐压可自动控制在设定值附近。In the tank pressure monitoring module, the control mode of tank pressure automatic control is analog PID fixed value control or analog PID sequence control, and the alarm mode is upper and lower limit deviation alarm or no alarm. Parameter setting: The setting value of the flow control, the upper limit deviation alarm value and the lower limit deviation alarm value can be set through the parameter setting function item on the computer. During automatic control: 1) Open the stop valve of the exhaust regulating valve and close the bypass valve; 2) After a few minutes, the tank pressure can be automatically controlled near the set value.
在本发明中,通过控制器对每个模块的实时监测控制,可随时独立调整各项发酵参数,使得发酵过程实现自动化。并且每个关键模块可在手动和自动模式下随时切换,使得发酵参数的调整更为灵活。In the present invention, through the real-time monitoring and control of each module by the controller, various fermentation parameters can be adjusted independently at any time, so that the fermentation process can be automated. And each key module can be switched between manual and automatic modes at any time, making the adjustment of fermentation parameters more flexible.
以上所述的具体实施方式,对本发明的目的、技术方案和有益效果进行了进一步详细说明,所应理解的是,以上所述仅为本发明的具体实施方式而已,并不用于限定本发明的保护范围,凡在本发明的精神和原则之内,所做的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。The specific embodiments described above have further described the purpose, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above descriptions are only specific embodiments of the present invention and are not intended to limit the scope of the present invention. Protection scope, within the spirit and principles of the present invention, any modification, equivalent replacement, improvement, etc., shall be included in the protection scope of the present invention.
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