CN108226147B - Urine microalbumin detection reagent strip and preparation method thereof - Google Patents
Urine microalbumin detection reagent strip and preparation method thereof Download PDFInfo
- Publication number
- CN108226147B CN108226147B CN201711447304.2A CN201711447304A CN108226147B CN 108226147 B CN108226147 B CN 108226147B CN 201711447304 A CN201711447304 A CN 201711447304A CN 108226147 B CN108226147 B CN 108226147B
- Authority
- CN
- China
- Prior art keywords
- reagent
- filter paper
- paper sheet
- urine
- microalbumin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 67
- 210000002700 urine Anatomy 0.000 title claims abstract description 44
- 238000001514 detection method Methods 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 239000000243 solution Substances 0.000 claims abstract description 23
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims abstract description 15
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims abstract description 9
- FXHOOIRPVKKKFG-UHFFFAOYSA-N N,N-Dimethylacetamide Chemical compound CN(C)C(C)=O FXHOOIRPVKKKFG-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims abstract description 9
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229920004890 Triton X-100 Polymers 0.000 claims abstract description 9
- 239000013504 Triton X-100 Substances 0.000 claims abstract description 9
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 9
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 9
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 9
- 238000012360 testing method Methods 0.000 claims abstract description 9
- LEAHFJQFYSDGGP-UHFFFAOYSA-K trisodium;dihydrogen phosphate;hydrogen phosphate Chemical compound [Na+].[Na+].[Na+].OP(O)([O-])=O.OP([O-])([O-])=O LEAHFJQFYSDGGP-UHFFFAOYSA-K 0.000 claims abstract description 9
- 239000007853 buffer solution Substances 0.000 claims abstract description 5
- 239000008055 phosphate buffer solution Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 11
- 239000008213 purified water Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005303 weighing Methods 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 238000001291 vacuum drying Methods 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
- 229920005989 resin Polymers 0.000 claims description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 206010027525 Microalbuminuria Diseases 0.000 claims 1
- 238000002558 medical inspection Methods 0.000 abstract description 2
- 108010088751 Albumins Proteins 0.000 description 10
- 102000009027 Albumins Human genes 0.000 description 10
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- 230000029142 excretion Effects 0.000 description 3
- 210000000585 glomerular basement membrane Anatomy 0.000 description 3
- 208000017169 kidney disease Diseases 0.000 description 3
- 230000003907 kidney function Effects 0.000 description 3
- 238000002310 reflectometry Methods 0.000 description 3
- 230000002485 urinary effect Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 208000007342 Diabetic Nephropathies Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 238000008156 Microalbumin Assay Methods 0.000 description 1
- 206010033645 Pancreatitis Diseases 0.000 description 1
- 206010033647 Pancreatitis acute Diseases 0.000 description 1
- 206010061481 Renal injury Diseases 0.000 description 1
- 201000003229 acute pancreatitis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007705 chemical test Methods 0.000 description 1
- 238000013098 chemical test method Methods 0.000 description 1
- 208000033679 diabetic kidney disease Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 201000011461 pre-eclampsia Diseases 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000003904 radioactive pollution Methods 0.000 description 1
- 231100001028 renal lesion Toxicity 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a urine microalbumin detection reagent strip and a preparation method thereof, belonging to the technical field of medical inspection. The reagent strip comprises a supporting plate and a filter paper sheet, wherein the filter paper sheet is attached to the supporting plate, a dye solution is immersed in the filter paper sheet, the dye solution consists of a reagent I and a reagent II, and the reagent I consists of cyanine dye, triethanolamine buffer solution, Triton X-100, sodium benzoate and PEG-4000; the reagent II comprises disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, O- (N-succinimidyl) -N N 'N' -tetramethyl tetrafluoroborate urea, sodium azide and dimethylacetamide. The cyanine dye adopted by the urine microalbumin detection reagent strip prepared by the invention can rapidly react with microalbumin in urine, and when the concentration of the microalbumin is in the range of 0-200mg/L, the test paper presents an obvious color gradation from colorless to dark blue, is easy to visually distinguish and read by machine, and has accurate determination result and good stability.
Description
Technical Field
The invention belongs to the technical field of medical inspection, and particularly relates to a urine microalbumin detection reagent strip and a preparation method thereof.
Background
Albumin is one of the important plasma proteins, and normally, the molecular weight of albumin is large and cannot cross glomerular basement membranes, so that albumin is contained in healthy human urine only at a very low concentration, specifically, not more than 20mg of albumin per liter of urine, and is also called urine microalbumin test. In the case of disease, the permeability of the glomerular basement membrane is altered by damage, and any disease that can cause increased permeability of the glomerular basement membrane can lead to the excretion of albumin. At this time, albumin can enter urine, and the urine albumin concentration can continuously rise. The most ideal method for measuring the urinary albumin is to leave a 24-hour urine sample, and because the excretion of the urinary albumin has great variation, the excretion of the urine albumin in one time of the urine sample without timing is increased, which may not be meaningful, and the method has diagnostic value by continuously increasing 2-3 times.
The microalbumin assay reflects the early renal disease and renal injury. Pathological increases are seen in diabetic nephropathy, hypertension, and preeclampsia during pregnancy. Early urinary microalbumin is an early sign and precursor to the development of renal disease, when kidney damage is in a reversible stage, if timely treated, it can stop or reverse the progression of renal disease. The urine microalbumin detection can be used as a renal function index of systemic or local inflammatory reaction, such as early renal lesion caused by urinary tract infection and the like; a prediction index of acute pancreatitis complications; the urine microalbumin can be detected by a patient taking the medicine with the influence on the renal function, so that the renal function condition can be observed early and measures can be taken early.
The currently applied urine microalbumin detection methods in China mainly comprise a dry chemical detection method, an immunofluorescence method, an enzyme immunoassay method, a radioimmunoassay and an immunoturbidimetry method. The dry chemical test method can provide semi-quantitative or qualitative results, and meanwhile, the detection result of the dry chemical test strip is influenced by more factors, and false negative and false positive results can be inevitably generated in clinical tests. The immunofluorescence method and the enzyme immunoassay method are complex in operation, long in time consumption and inconvenient for wide clinical application; the radioimmunoassay has high sensitivity and strong specificity, but has the defects of radioactive pollution, short reagent storage life and the like. The immunoturbidimetry method has the advantages of simple operation, short measuring period and high precision, and is widely used on a biochemical analyzer. However, the detection principle of the immunoturbidimetry is that an insoluble antigen-antibody complex is generated by reaction and generates a certain turbidity, and the level of the turbidity reflects the content of the microalbumin in a sample, so that the defect of low sensitivity inevitably occurs in the conventional urine microalbumin kit.
Disclosure of Invention
The invention aims to provide a urine microalbumin detection reagent strip and a preparation method thereof.
In order to achieve the above object, the present invention adopts the following technical solutions.
A urine microalbumin detection reagent strip comprises a supporting plate and a filter paper sheet, wherein the filter paper sheet is attached to the supporting plate, a dye solution is immersed in the filter paper sheet, the dye solution consists of a reagent I and a reagent II, and the reagent I consists of 0.10-100mmol/L of cyanine dye, pH7.4-8.0, 20-200mmol/L of triethanolamine buffer solution, 0.01-1g/L of Triton X-100, 0.10-10g/L of sodium benzoate and 0.10-20g/L of PEG-4000; the reagent II consists of pH7.4.0-8.0, 50-200mmol/L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, 0.01-50mmol/L O- (N-succinimidyl) -N N 'N' -tetramethyltetrafluoroborate urea, 0.10-20g/L sodium azide and 1-20g/L dimethylacetamide; the cyanine dye is a compound of formula I,
the invention also aims to provide a preparation method of the urine microalbumin detection reagent strip, which comprises the following steps:
weighing raw materials including cyanine dye, triethanolamine, Triton X-100, sodium benzoate and PEG-4000, adding the weighed materials into purified water to reach a constant volume of 1L, and adjusting pH to obtain a reagent I; weighing disodium hydrogen phosphate-sodium dihydrogen phosphate, O- (N-succinimidyl) -N N 'N' -tetramethyltetrafluoroborate urea, sodium azide and dimethylacetamide, adding into purified water to reach a constant volume of 1L, and adjusting the pH value to obtain a reagent II;
mixing the reagent I and the reagent II, placing the mixture in a closed environment at room temperature for 1-2 hours to obtain a dye solution, immersing a filter paper sheet into the dye solution, taking out the immersed dye solution after full immersion, placing the immersed dye solution in a vacuum drying oven at the temperature of 50-70 ℃ for drying for 10-20min, fixing the dried filter paper sheet and a supporting plate, cutting the filter paper sheet into rectangular paper strips with the length of 10mm and the width of 5mm, and preparing the urine microalbumin detection reagent strips.
The supporting plate of the urine microalbumin detection reagent strip is polyvinyl chloride resin, and the filter paper sheet is common quantitative filter paper or common qualitative filter paper.
The invention has the beneficial technical effects that: the cyanine dye adopted by the urine microalbumin detection reagent strip prepared by the invention can rapidly react with microalbumin in urine, and when the concentration of the microalbumin is in the range of 0-200mg/L, the test paper presents an obvious color gradation from colorless to dark blue, is easy to visually distinguish and read by machine, and has accurate determination result and good stability.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
Examples
A urine microalbumin detection reagent strip comprises a support plate and a filter paper sheet, wherein the filter paper sheet is attached to the support plate, a dye solution is immersed in the filter paper sheet, the dye solution consists of a reagent I and a reagent II, and the reagent I consists of 0.10mmol/L cyanine dye, pH7.4, 20mmol/L triethanolamine buffer solution, 0.01g/L Triton X-100, 0.10g/L sodium benzoate and 0.10g/L PEG-4000; the reagent II consists of pH7.4.0, 50mmol/L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, 0.01mmol/L O- (N-succinimidyl) -N N 'N' -tetramethyl tetrafluoroborate urea, 0.10g/L sodium azide and 1g/L dimethylacetamide; the cyanine dye is a compound of formula I,
the preparation method of the urine microalbumin detection reagent strip comprises the following steps:
weighing raw materials including cyanine dye, triethanolamine, Triton X-100, sodium benzoate and PEG-4000, adding the weighed materials into purified water to reach a constant volume of 1L, and adjusting pH to obtain a reagent I; weighing disodium hydrogen phosphate-sodium dihydrogen phosphate, O- (N-succinimidyl) -N N 'N' -tetramethyltetrafluoroborate urea, sodium azide and dimethylacetamide, adding into purified water to reach a constant volume of 1L, and adjusting the pH value to obtain a reagent II;
mixing the reagent I and the reagent II, placing the mixture in a closed manner at room temperature for 1.5 hours to obtain a dye solution, immersing a common quantitative filter paper sheet into the dye solution, taking out the common quantitative filter paper sheet after full immersion, placing the common quantitative filter paper sheet in a vacuum drying box at the temperature of 60 ℃ for drying for 15min, fixing the dried filter paper sheet and a PMMA resin supporting plate, cutting the filter paper sheet into rectangular paper strips with the length of 10mm and the width of 5mm, and preparing the urine microalbumin detection reagent strips.
The method for detecting the microalbumin in urine by using the microalbumin urine detection reagent strip comprises the following steps:
firstly, preparing bovine serum albumin standard solutions of 0mg/L, 10mg/L, 20mg/L, 40mg/L, 80mg/L, 100mg/L and 200 mg/L; then, the test reagent strips prepared in the embodiment are respectively immersed in the 7 bovine serum albumin standard solutions; then measuring the reflectivity for 1 minute by using a urine analyzer, and finally drawing a standard curve according to 7 points;
when the reagent strip is used, the reagent strip for detecting microalbumin in urine prepared according to the embodiment is immersed in urine to be detected and taken out, the urine analyzer is used for detecting the reflectivity after 1 minute, and the detected reflectivity and the corresponding concentration on the standard curve are the microalbumin concentration of the liquid to be detected.
Example 2
A urine microalbumin detection reagent strip comprises a supporting plate and a filter paper sheet, wherein the filter paper sheet is attached to the supporting plate, a dye solution is immersed in the filter paper sheet, the dye solution consists of a reagent I and a reagent II, and the reagent I consists of 10mmol/L cyanine dye, pH8.0, 100mmol/L triethanolamine buffer solution, 0.1g/L Triton X-100, 1g/L sodium benzoate and 2g/L PEG-4000; the reagent II consists of 100mmol/L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, 1mmol/LO- (N-succinimidyl) -N N 'N' -tetramethyltetrafluoroborate urea, 2g/L sodium azide and 10g/L dimethylacetamide; the cyanine dye is a compound of formula I,
the preparation method of the urine microalbumin detection reagent strip comprises the following steps:
weighing raw materials including cyanine dye, triethanolamine, Triton X-100, sodium benzoate and PEG-4000, adding the weighed materials into purified water to reach a constant volume of 1L, and adjusting pH to obtain a reagent I; weighing disodium hydrogen phosphate-sodium dihydrogen phosphate, O- (N-succinimidyl) -N N 'N' -tetramethyltetrafluoroborate urea, sodium azide and dimethylacetamide, adding into purified water to reach a constant volume of 1L, and adjusting the pH value to obtain a reagent II;
mixing the reagent I and the reagent II, placing the mixture in a closed manner at room temperature for 2 hours to obtain a dye solution, soaking a common qualitative filter paper sheet into the dye solution, taking out the common qualitative filter paper sheet after full soaking, placing the common qualitative filter paper sheet in a vacuum drying oven at the temperature of 65 ℃ for drying for 20 minutes, fixing the dried filter paper sheet and a PVC supporting plate, cutting the filter paper sheet into rectangular paper strips with the length of 10mm and the width of 5mm, and preparing the urine microalbumin detection reagent strips.
The method for detecting the microalbumin in urine by using the microalbumin urine detection reagent strip comprises the following steps:
firstly, preparing bovine serum albumin standard solutions of 0mg/L, 10mg/L, 20mg/L, 40mg/L, 80mg/L, 100mg/L and 200 mg/L; then, the test reagent strips prepared in the embodiment are respectively immersed in the 7 bovine serum albumin standard solutions; then taking out the test paper for airing after 2 seconds, completely developing after 1 minute, and finally preparing a color comparison card according to the color development condition of each test paper, wherein the color scales of the color comparison card are 7 white to dark blue;
when using, will soak the urine microalbumin detection reagent strip prepared according to the embodiment and take out in the urine that awaits measuring, take out after 2 seconds and dry, the development is complete after 1 minute, compare with standard colour comparison card, the concentration that the colour that corresponds on the standard colour comparison card that is closest with the colour on the detection reagent strip is the concentration of the liquid that awaits measuring, if between two colour scales, then can estimate and read microalbumin concentration.
Claims (3)
1. The reagent strip for detecting the microalbumin in urine comprises a supporting plate and a filter paper sheet, wherein the filter paper sheet is attached to the supporting plate, and a dye solution is immersed in the filter paper sheet, and is characterized in that: the dye solution consists of a reagent I and a reagent II, wherein the reagent I consists of 0.10-100mmol/L cyanine dye, pH7.4-8.0, 20-200mmol/L triethanolamine buffer solution, 0.01-1g/L Triton X-100, 0.10-10g/L sodium benzoate and 0.10-20g/L PEG-4000; the reagent II consists of pH7.4.0-8.0, 50-200mmol/L disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, 0.01-50mmol/L O- (N-succinimidyl) -N N 'N' -tetramethyltetrafluoroborate urea, 0.10-20g/L sodium azide and 1-20g/L dimethylacetamide; the cyanine dye is a compound of formula I,
2. a method for preparing the urine microalbumin test reagent strip according to claim 1, comprising the steps of:
weighing raw materials including cyanine dye, triethanolamine, Triton X-100, sodium benzoate and PEG-4000, adding the weighed materials into purified water to reach a constant volume of 1L, and adjusting pH to obtain a reagent I; weighing disodium hydrogen phosphate-sodium dihydrogen phosphate, O- (N-succinimidyl) -N N 'N' -tetramethyltetrafluoroborate urea, sodium azide and dimethylacetamide, adding into purified water to reach a constant volume of 1L, and adjusting the pH value to obtain a reagent II;
mixing the reagent I and the reagent II, placing the mixture in a closed environment at room temperature for 1-2 hours to obtain a dye solution, immersing a filter paper sheet into the dye solution, taking out the immersed dye solution after full immersion, placing the immersed dye solution in a vacuum drying oven at the temperature of 50-70 ℃ for drying for 10-20min, fixing the dried filter paper sheet and a supporting plate, cutting the filter paper sheet into rectangular paper strips with the length of 10mm and the width of 5mm, and preparing the urine microalbumin detection reagent strips.
3. The method for preparing a reagent strip for detecting microalbuminuria as claimed in claim 2, wherein the method comprises the following steps: the supporting plate is PMMA or PVC resin; the filter paper sheet is common quantitative filter paper or common qualitative filter paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711447304.2A CN108226147B (en) | 2017-12-27 | 2017-12-27 | Urine microalbumin detection reagent strip and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711447304.2A CN108226147B (en) | 2017-12-27 | 2017-12-27 | Urine microalbumin detection reagent strip and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108226147A CN108226147A (en) | 2018-06-29 |
| CN108226147B true CN108226147B (en) | 2021-06-04 |
Family
ID=62649061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711447304.2A Active CN108226147B (en) | 2017-12-27 | 2017-12-27 | Urine microalbumin detection reagent strip and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108226147B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113376150A (en) * | 2021-06-10 | 2021-09-10 | 吉林基蛋生物科技有限公司 | Urine microalbumin dry chemical detection test paper and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091316A (en) * | 2013-01-24 | 2013-05-08 | 中国科学院化学研究所 | Urine microalbumin detection method and kit |
| CN103901034A (en) * | 2014-04-29 | 2014-07-02 | 中国科学院苏州生物医学工程技术研究所 | Detection reagent strip for detecting microalbumin in urine and preparation method of detection reagent strip |
| GB2521119A (en) * | 2013-10-30 | 2015-06-17 | Surescreen Diagnostics Ltd | Indication device |
| CN106053368A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining microalbuminuria and preparation method thereof |
-
2017
- 2017-12-27 CN CN201711447304.2A patent/CN108226147B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091316A (en) * | 2013-01-24 | 2013-05-08 | 中国科学院化学研究所 | Urine microalbumin detection method and kit |
| GB2521119A (en) * | 2013-10-30 | 2015-06-17 | Surescreen Diagnostics Ltd | Indication device |
| CN103901034A (en) * | 2014-04-29 | 2014-07-02 | 中国科学院苏州生物医学工程技术研究所 | Detection reagent strip for detecting microalbumin in urine and preparation method of detection reagent strip |
| CN106053368A (en) * | 2016-07-12 | 2016-10-26 | 安徽伊普诺康生物技术股份有限公司 | Kit for determining microalbuminuria and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 新型水溶性不对称五甲川菁染料的合成、;王丽等;《高等学校化学学报》;20100731;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108226147A (en) | 2018-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105388146B (en) | Kit that is a kind of while detecting sodium in urine, creatinine and microalbumin | |
| Pergande et al. | Sandwich enzyme immunoassay of cystatin C in serum with commercially available antibodies | |
| Nelson et al. | Direct determination of free thyroxin in undiluted serum by equilibrium dialysis/radioimmunoassay. | |
| US5516700A (en) | Automated urinalysis method | |
| CN105334211B (en) | It is a kind of while detect the kit of sodium and creatinine in urine | |
| CN109856128A (en) | A kind of urine glucose detection test paper and preparation method thereof of ascorbic acid interference | |
| CN1912594A (en) | Chemiluminescence investigating method of creatinine in serum | |
| CN108226147B (en) | Urine microalbumin detection reagent strip and preparation method thereof | |
| US5759860A (en) | Automated analysis method for detecting bacterial nitrite in urine | |
| CN113376150A (en) | Urine microalbumin dry chemical detection test paper and preparation method thereof | |
| CN110398586B (en) | Fructosamine assay kit | |
| Klatskin et al. | The significance of the “one-minute”(prompt direct reacting) bilirubin in serum | |
| Christensen et al. | Three highly sensitive" bedside" serum and urine tests for pregnancy compared | |
| KR20000029575A (en) | Method for examining chronic rejection reactions following organ transplantation and method for determining urine components | |
| CN111505303A (en) | Kit for detecting heart-type fatty acid binding protein by chemiluminescence method and use method thereof | |
| Phillipou et al. | Screening for microalbuminuria by use of a rapid, low-cost colorimetric assay. | |
| US5776780A (en) | Method for quantitatively measuring white blood cells esterase activity in urine | |
| Wu et al. | A simple radial immunodiffusion method for assay of beta 2-microglobulin in serum. | |
| CN213544591U (en) | Detection test paper | |
| Sonntag | Dry chemistry: analysis with carrier-bound reagents | |
| Alinovi et al. | Competitive enzyme-linked immunosorbent assay (CELISA) of urinary albumin. | |
| US5736408A (en) | Method for the detection of urobilinogen in urine on an automated analyzer | |
| Ohkubo et al. | Multilayer-film analysis for urea nitrogen in blood, serum, or plasma. | |
| CN113063947B (en) | Kit, system and method for detecting serum amyloid A | |
| CN118706772B (en) | An anti-interference urine microalbumin assay kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Hongkui Inventor after: Yan Hongjuan Inventor after: Lu Yao Inventor after: Du Wei Inventor after: Zhao Bin Inventor before: Li Hongkui |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |