CN108226016A - The mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup - Google Patents
The mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup Download PDFInfo
- Publication number
- CN108226016A CN108226016A CN201810030643.9A CN201810030643A CN108226016A CN 108226016 A CN108226016 A CN 108226016A CN 201810030643 A CN201810030643 A CN 201810030643A CN 108226016 A CN108226016 A CN 108226016A
- Authority
- CN
- China
- Prior art keywords
- antibody
- mass spectrum
- flow cytometer
- cytometer detection
- tumor vaccine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 title claims abstract description 13
- 238000001819 mass spectrum Methods 0.000 title claims abstract description 13
- 229960005486 vaccine Drugs 0.000 title claims abstract description 10
- 230000003834 intracellular effect Effects 0.000 claims description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 229910052751 metal Inorganic materials 0.000 abstract description 5
- 239000002184 metal Substances 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 20
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 9
- 229940098773 bovine serum albumin Drugs 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 108060003951 Immunoglobulin Proteins 0.000 description 3
- 238000004040 coloring Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 102000018358 immunoglobulin Human genes 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241000699800 Cricetinae Species 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012083 mass cytometry Methods 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Landscapes
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of mass spectrum flow cytometer detection kits of the accurate parting of tumor vaccine cells subgroup, are made of 42 kinds of antibody with metal label.The present invention develops the kit of 42 channels, 42 kinds of antibody, using mass spectrum flow cytometer detection, can once detect 42 kinds of antibody simultaneously, realize to the accurate parting of tumor vaccine cells subgroup.Kit of the present invention after tested, can realize all immunocyte subgroup systems, comprehensive, dynamic, the accurately parting, and without the dye control of each channel list, do not need to calculate compensation, save sample of tumor disease.
Description
Technical field
The present invention relates to technical field of cell biology, and in particular to a kind of matter of the accurate parting of tumor vaccine cells subgroup
Compose flow cytometer detection kit.
Background technology
Immune system plays an important role during the generation, development, prognosis of disease.But current research means
Have focused largely on the function and molecular mechanism for studying several immunocyte subgroups in disease.Simultaneously as technological means lacks
It falls into, causes previous research not accurate enough to the definition of all kinds of immunocyte subgroups, result of study is excessively unilateral.Such as conventional flow
Formula cell technology, the overlapping quantity for limiting fluorescence channel of fluorescence signal increase, and number of active lanes is few, and the transmitting light of channel
Overlapping is composed, needs to set single dye group of blank group, negative control group and each channel, carries out complicated compensation calculation, sample
Consumption is huge.
Mass spectrum Flow Cytometry (Mass Cytometry, CyTOF) is the slender extracellular molecule just to have grown up in recent years
Typing method.Its use metallic element as the label of antibody molecule and using ICP Mass Spectrometer Methods system to slender cellular surface and
Internal multiple protein molecule is carried out at the same time detection.Compared with conventional flow cell technology, have that sense channel is more, interchannel is without doing
It disturbs, the features such as signal accuracy is high and flux is big.
Although the mass spectrum flow cytometer of Fluidigm companies of the U.S. is powerful, and possesses 600 various metals and mark in advance
The antibody of note, still, currently for the research of the immunocyte subunit cluster analysis of blood of cancer patients, without ready-made kit
It can directly use, and number of active lanes also only has 35.Preliminary making antibody purchasing channel is single, expensive and time-consuming.
The country is also without the research and development and production of similar reagents box product.
Therefore, in the immune system research to tumor disease, lack to all immunocyte subgroup systems, comprehensive
, it is dynamic, accurately describe.
Invention content
It is an object of the present invention to provide a kind of mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup, mainly
Mass spectrum flow cytometer detection is carried out for the immunocyte subgroup of blood of cancer patients, achievees the purpose that accurate parting, it is existing to solve
There is the deficiency of technology.
The present invention uses following technical scheme：
A kind of mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup, it is anti-with metal label by 42 kinds
Body forms, shown in table specific as follows：
Wherein, number 3,24,25,31,32,34,36 is intracellular antibody, and other is extracellular antibody.
Beneficial effects of the present invention：
The present invention develops the kit of 42 channels, 42 kinds of antibody, using mass spectrum flow cytometer detection, can once detect simultaneously
42 kinds of antibody are realized to the accurate parting of tumor vaccine cells subgroup.Kit of the present invention after tested, can realize tumor disease
All immunocyte subgroup systems, comprehensive, dynamic, accurately parting, and without the dye control of each channel list, not needing to
Compensation is calculated, saves sample.
Description of the drawings
Fig. 1 to Fig. 4 is 42 kinds of antibody coloring figures in embodiment 1.
Specific embodiment
The present invention is done with reference to embodiment and attached drawing and is further explained.The following example is merely to illustrate this hair
It is bright, but it is not used to limit the practical range of the present invention.
A kind of mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup, it is anti-with metal label by 42 kinds
Body forms, specific as shown in table 1：
Table 1
For 42 kinds of antibody in kit using 42 channels, number 3,24,25,31,32,34,36 is intracellular antibody, other
For extracellular antibody, all T cell subgroups can be identified, includingT、central memory T、effector T、
Effector memory CD4+T and CD8+T and B cell subgroup etc..
Embodiment 1 tests the coloring of 42 kinds of antibody
1st, prepare fresh normal human peripheral blood, extract immunocyte.
2nd, immunocyte is divided into two groups of control group and experimental group, every group of about 3x10^6 cell, respectively with PBS weights
It is outstanding, volume is adjusted to 1mL, adds in 194Pt, room temperature dyeing 2min distinguishes cell anyway.
3rd, 2mL bovine serum albumin solutions are added in, 500g/5min centrifugations absorb supernatant, add in 50uL confining liquids (0.5uL
Human immunoglobulin(HIg) solution, 0.5uL mouse immune globulins solution, 0.5uL rat immunoglobulins solution, 0.5uL hamsters are exempted from
Epidemic disease globulin solution, 48uL bovine serum albumin solutions), 20min is closed on ice.
4th, control group adds in 50uL bovine serum albumin solutions as blank control, and experimental group adds in the extracellular antibody of 50uL and mixes
Liquid (35 kinds of extracellular each 0.5ul of antibody, bovine serum albumin solution 32.5ul in table 1) is closed, cell is resuspended, dyes 30min on ice.
5th, 2mL bovine serum albumin solutions are added in, supernatant is absorbed in 500g/5min centrifugations, is added in slender containing 0.5v/v ‰
The fixation of born of the same parents' indicator 191/193Ir (201192B, fluidigm)-rupture of membranes mixed liquor (fix andperm buffer,
Fluidigm, 201067) 1ml, cell is resuspended, 4 DEG C are overnight.
6th, 2mL bovine serum albumin solutions are added in, supernatant is absorbed in 800g/5min centrifugations；It is molten that control group adds in 50uL ruptures of membranes
Liquid (perm buffer, eBioscienceTMPermeabilization Buffer (10X), 00-8333-56, first spend from
Sub- water is diluted to 1x, then uses) as blank control, experimental group adds in 50uL intracellular antibodies mixed liquor (7 kinds of intracellulars in table 1
Antibody each 0.5ul, bovine serum albumin solution 46.5ul), cell is resuspended, places 30min on ice；
7th, 2mL bovine serum albumin solutions are added in, supernatant is absorbed in 800g/5min centrifugations.
8th, 2mL bovine serum albumin solutions are added in, supernatant is absorbed in 800g/5min centrifugations.
9th, 2mL deionized waters are added in, supernatant is absorbed in 800g/5min centrifugations.
10th, 2mL deionized waters are added in, supernatant is absorbed in 800g/5min centrifugations.
11st, sample filtering, cell count adjust volume, machine in preparations, progress mass spectrum flow cytometer detection.
12nd, as shown in Figures 1 to 4,42 kinds of antibody coloring figures, i.e., experimental group is compared with control group for result.Frame centre circle
Out be that the positive cell group of every a kind of antibody accounts for the percentage of total number of cells.Compared with the control group, the immune of antibody is added in
Cell is divided into negative and positive two groups, it was demonstrated that antibody label effect is good, can separate aim cell and non-aim cell, resists
Body can be used for subsequent experimental.
Claims (1)
1. a kind of mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup, which is characterized in that by 42 kinds of band gold
Belong to the antibody composition of label, shown in table specific as follows：
Wherein, number 3,24,25,31,32,34,36 is intracellular antibody, and other is extracellular antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810030643.9A CN108226016A (en) | 2018-01-12 | 2018-01-12 | The mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810030643.9A CN108226016A (en) | 2018-01-12 | 2018-01-12 | The mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108226016A true CN108226016A (en) | 2018-06-29 |
Family
ID=62640293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810030643.9A Pending CN108226016A (en) | 2018-01-12 | 2018-01-12 | The mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108226016A (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110412287A (en) * | 2019-07-11 | 2019-11-05 | 上海宸安生物科技有限公司 | One kind being based on single celled immunocyte parting quantitative analysis method |
| CN110412286A (en) * | 2019-07-11 | 2019-11-05 | 上海宸安生物科技有限公司 | A method of Single cell analysis being carried out to tumor sample using mass spectrum streaming systems |
| CN110608991A (en) * | 2019-09-09 | 2019-12-24 | 浙江普罗亭健康科技有限公司 | Cell cycle detection kit and detection method based on mass spectrometry flow detection technology |
| CN112147326A (en) * | 2020-09-04 | 2020-12-29 | 北京大学 | An accurate detection kit for tumor immune cell subset typing |
| CN113109575A (en) * | 2021-04-16 | 2021-07-13 | 浙江普罗亭健康科技有限公司 | 40 antibody kit for monitoring human immune state and application |
| CN113125755A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 9 antibody kit for monitoring human immune state and application thereof |
| CN113125754A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 23 antibody kit for monitoring human immune state and application |
| CN113125719A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 6 antibody kit for monitoring human immune state and application |
| CN113125733A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 42 antibody kit for monitoring human immune state and application thereof |
| CN113125718A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 45 antibody kit for monitoring human immune state and application |
| CN114112868A (en) * | 2020-08-26 | 2022-03-01 | 上海睿昂基因科技股份有限公司 | Flow cytometry kit for monitoring tumor-related immune microenvironment and monitoring method |
| CN114720358A (en) * | 2022-04-11 | 2022-07-08 | 浙江普罗亭健康科技有限公司 | Antibody combination for replacing side scattered light signals in mass spectrum flow type blood tumor immunophenotyping and application |
| CN115166252A (en) * | 2022-07-07 | 2022-10-11 | 泰州宸安生物科技有限公司 | Lymphocyte subgroup classification and quantitative detection kit, detection method and application thereof |
| CN115792200A (en) * | 2022-11-18 | 2023-03-14 | 无锡市人民医院 | Antibody combination and kit for absolute counting of peripheral blood B cell subsets after B cell clearance treatment and application of antibody combination and kit |
| CN116794313A (en) * | 2023-08-18 | 2023-09-22 | 江西赛基生物技术有限公司 | Kit and method for simultaneously detecting three tumor markers based on flow cytometry |
Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003049697A2 (en) * | 2001-12-10 | 2003-06-19 | Rhode Island Hospital | Treatment of glioblastoma with thymosin-alpha 1 |
| WO2004040314A1 (en) * | 2002-10-31 | 2004-05-13 | Chemometec A/S | A method for assessment of particles |
| US20080274091A1 (en) * | 2005-05-20 | 2008-11-06 | Virxsys Corporation | Autologous T Cell Manufacturing Processes |
| US20110262468A1 (en) * | 2010-04-23 | 2011-10-27 | Nodality, Inc. | Method for Monitoring Vaccine Response Using Single Cell Network Profiling |
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| WO2012151578A1 (en) * | 2011-05-05 | 2012-11-08 | Baylor Research Institute | Immunoglobulin-like transcript (ilt) receptors as cd8 antagonists |
| EP2630967A1 (en) * | 2007-10-12 | 2013-08-28 | Massachusetts Institute of Technology | Vaccine nanotechnology |
| CN104142315A (en) * | 2013-05-07 | 2014-11-12 | 中国科学院理化技术研究所 | Surface plasma resonance sensing chip for detecting tumor cells and preparation method and application thereof |
| CN104360050A (en) * | 2014-09-22 | 2015-02-18 | 重庆医科大学附属儿童医院 | Method for lymphocyte subsets and kit thereof |
| CN104360049A (en) * | 2014-09-22 | 2015-02-18 | 重庆医科大学附属儿童医院 | T lymphocyte immunophenotyping method and kit |
| CN104360053A (en) * | 2014-09-22 | 2015-02-18 | 重庆医科大学附属儿童医院 | B lymphocyte immunophenotyping method and kit |
| US20170044265A1 (en) * | 2015-06-24 | 2017-02-16 | Janssen Biotech, Inc. | Immune Modulation and Treatment of Solid Tumors with Antibodies that Specifically Bind CD38 |
| US20170145381A1 (en) * | 2014-06-06 | 2017-05-25 | Fuwan Pty Ltd | Method of generating multilineage potential cells from lymphocytes |
| US20170173176A1 (en) * | 2015-07-15 | 2017-06-22 | Suzhou M-Conj Biotech Co., Ltd. | Acetylenedicarboxyl linkers and their uses in specific conjugation of a cell-binding molecule |
| CN107290537A (en) * | 2016-08-02 | 2017-10-24 | 北京金沐医疗科技有限公司 | Detect the flow cytometer detection kit of tumor markers |
| US20170370934A1 (en) * | 2013-10-25 | 2017-12-28 | Nodality, Inc. | Methods and compositions for immunomodulation |
| WO2018005378A1 (en) * | 2016-06-28 | 2018-01-04 | Kun-Chih Tsai | Method for detecting and isolating invasive cancer stem cells employing cell-surface amfr and the use thereof |
-
2018
- 2018-01-12 CN CN201810030643.9A patent/CN108226016A/en active Pending
Patent Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003049697A2 (en) * | 2001-12-10 | 2003-06-19 | Rhode Island Hospital | Treatment of glioblastoma with thymosin-alpha 1 |
| WO2004040314A1 (en) * | 2002-10-31 | 2004-05-13 | Chemometec A/S | A method for assessment of particles |
| US20080274091A1 (en) * | 2005-05-20 | 2008-11-06 | Virxsys Corporation | Autologous T Cell Manufacturing Processes |
| EP2630967A1 (en) * | 2007-10-12 | 2013-08-28 | Massachusetts Institute of Technology | Vaccine nanotechnology |
| CN102245201A (en) * | 2008-10-12 | 2011-11-16 | 麻省理工学院 | Adjuvant incorporation in immunonanotherapeutics |
| US20110262468A1 (en) * | 2010-04-23 | 2011-10-27 | Nodality, Inc. | Method for Monitoring Vaccine Response Using Single Cell Network Profiling |
| WO2012151578A1 (en) * | 2011-05-05 | 2012-11-08 | Baylor Research Institute | Immunoglobulin-like transcript (ilt) receptors as cd8 antagonists |
| CN104142315A (en) * | 2013-05-07 | 2014-11-12 | 中国科学院理化技术研究所 | Surface plasma resonance sensing chip for detecting tumor cells and preparation method and application thereof |
| US20170370934A1 (en) * | 2013-10-25 | 2017-12-28 | Nodality, Inc. | Methods and compositions for immunomodulation |
| US20170145381A1 (en) * | 2014-06-06 | 2017-05-25 | Fuwan Pty Ltd | Method of generating multilineage potential cells from lymphocytes |
| CN104360053A (en) * | 2014-09-22 | 2015-02-18 | 重庆医科大学附属儿童医院 | B lymphocyte immunophenotyping method and kit |
| CN104360049A (en) * | 2014-09-22 | 2015-02-18 | 重庆医科大学附属儿童医院 | T lymphocyte immunophenotyping method and kit |
| CN104360050A (en) * | 2014-09-22 | 2015-02-18 | 重庆医科大学附属儿童医院 | Method for lymphocyte subsets and kit thereof |
| US20170044265A1 (en) * | 2015-06-24 | 2017-02-16 | Janssen Biotech, Inc. | Immune Modulation and Treatment of Solid Tumors with Antibodies that Specifically Bind CD38 |
| US20170173176A1 (en) * | 2015-07-15 | 2017-06-22 | Suzhou M-Conj Biotech Co., Ltd. | Acetylenedicarboxyl linkers and their uses in specific conjugation of a cell-binding molecule |
| WO2018005378A1 (en) * | 2016-06-28 | 2018-01-04 | Kun-Chih Tsai | Method for detecting and isolating invasive cancer stem cells employing cell-surface amfr and the use thereof |
| CN107290537A (en) * | 2016-08-02 | 2017-10-24 | 北京金沐医疗科技有限公司 | Detect the flow cytometer detection kit of tumor markers |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110412286A (en) * | 2019-07-11 | 2019-11-05 | 上海宸安生物科技有限公司 | A method of Single cell analysis being carried out to tumor sample using mass spectrum streaming systems |
| CN110412287A (en) * | 2019-07-11 | 2019-11-05 | 上海宸安生物科技有限公司 | One kind being based on single celled immunocyte parting quantitative analysis method |
| CN110608991A (en) * | 2019-09-09 | 2019-12-24 | 浙江普罗亭健康科技有限公司 | Cell cycle detection kit and detection method based on mass spectrometry flow detection technology |
| CN110608991B (en) * | 2019-09-09 | 2022-04-29 | 浙江普罗亭健康科技有限公司 | Cell cycle detection kit and detection method based on mass spectrometry |
| CN114112868A (en) * | 2020-08-26 | 2022-03-01 | 上海睿昂基因科技股份有限公司 | Flow cytometry kit for monitoring tumor-related immune microenvironment and monitoring method |
| CN114112868B (en) * | 2020-08-26 | 2024-12-13 | 上海睿昂基因科技股份有限公司 | A flow cytometry kit and monitoring method for monitoring tumor-related immune microenvironment |
| CN112147326A (en) * | 2020-09-04 | 2020-12-29 | 北京大学 | An accurate detection kit for tumor immune cell subset typing |
| CN113125755A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 9 antibody kit for monitoring human immune state and application thereof |
| CN113125718A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 45 antibody kit for monitoring human immune state and application |
| CN113125719A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 6 antibody kit for monitoring human immune state and application |
| CN113125754A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 23 antibody kit for monitoring human immune state and application |
| CN113125733A (en) * | 2021-04-16 | 2021-07-16 | 浙江普罗亭健康科技有限公司 | 42 antibody kit for monitoring human immune state and application thereof |
| CN113109575A (en) * | 2021-04-16 | 2021-07-13 | 浙江普罗亭健康科技有限公司 | 40 antibody kit for monitoring human immune state and application |
| WO2023197182A1 (en) * | 2022-04-11 | 2023-10-19 | 浙江普罗亭健康科技有限公司 | Antibody combination for replacing side scatter signal in mass cytometry hematological tumor immunophenotyping, and uses |
| CN114720358A (en) * | 2022-04-11 | 2022-07-08 | 浙江普罗亭健康科技有限公司 | Antibody combination for replacing side scattered light signals in mass spectrum flow type blood tumor immunophenotyping and application |
| CN114720358B (en) * | 2022-04-11 | 2022-09-27 | 浙江普罗亭健康科技有限公司 | Antibody combination for substituting side scattered light signals in mass spectrum flow type blood tumor immunology typing and application |
| JP7388787B2 (en) | 2022-04-11 | 2023-11-29 | ヂェァジィァン プルオッティング ヘルス テクノロジー カンパニー リミテッド | Antibody combinations for immunophenotyping of hematologic tumors by mass spectrometry flow cytometry |
| JP2023155889A (en) * | 2022-04-11 | 2023-10-23 | ヂェァジィァン プルオッティング ヘルス テクノロジー カンパニー リミテッド | Antibody combination to replace side-scattered light signals in mass spectrometry flow hematologic tumor immunophenotyping |
| CN115166252A (en) * | 2022-07-07 | 2022-10-11 | 泰州宸安生物科技有限公司 | Lymphocyte subgroup classification and quantitative detection kit, detection method and application thereof |
| CN115792200B (en) * | 2022-11-18 | 2023-09-15 | 无锡市人民医院 | An antibody combination, kit and application for absolute counting of peripheral blood B cell subpopulations after B cell depletion treatment |
| CN115792200A (en) * | 2022-11-18 | 2023-03-14 | 无锡市人民医院 | Antibody combination and kit for absolute counting of peripheral blood B cell subsets after B cell clearance treatment and application of antibody combination and kit |
| CN116794313A (en) * | 2023-08-18 | 2023-09-22 | 江西赛基生物技术有限公司 | Kit and method for simultaneously detecting three tumor markers based on flow cytometry |
| CN116794313B (en) * | 2023-08-18 | 2023-11-03 | 江西赛基生物技术有限公司 | Kit and method for simultaneously detecting three tumor markers based on flow cytometry |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108226016A (en) | The mass spectrum flow cytometer detection kit of the accurate parting of tumor vaccine cells subgroup | |
| Hartmann et al. | A universal live cell barcoding-platform for multiplexed human single cell analysis | |
| CN107796748B (en) | Detection method for single-cell mass spectrometry flow cytometry | |
| EP3485973B1 (en) | Microfluidic reagent card, detection method and application thereof | |
| DE69326956T2 (en) | IMPROVED METHOD FOR DETECTING SPECIFIC TARGET CELLS IN SPECIALIZED OR MIXED CELL POPULATION AND CONTAINING MIXED CELL POPULATIONS | |
| CN111527406B (en) | A method for preparing lymphocyte samples for flow cytometer analysis | |
| CN105807057B (en) | A method of capture is synchronous with identification circulating tumor cell to be carried out | |
| Lakhotia et al. | Damaging agitation intensities increase DNA synthesis rate and alter cell‐cycle phase distributions of CHO cells | |
| CN103592432B (en) | Method for separating sperm in sperm and epithelial cell mixed stain by using immunological magnetic beads | |
| WO2021012925A1 (en) | Method for measuring human peripheral blood lymphocytes | |
| CN110702589A (en) | Method for detecting exosome by flow cytometer | |
| CN110389219A (en) | A kind of enrichment detecting method of Epithelial and stromal mixed type and PD-L1 positive circulating tumor cell | |
| CN114720358B (en) | Antibody combination for substituting side scattered light signals in mass spectrum flow type blood tumor immunology typing and application | |
| CN105223360B (en) | Kit for detection and identification of normal plasma cell and clonal plasma cell and application thereof | |
| Li et al. | A microfluidic device for enhanced capture and high activity release of heterogeneous CTCs from whole blood | |
| Koolwijk et al. | Enrichment and selection of hybrid hybridomas by Percoll density gradient centrifugation and fluorescent-activated cell sorting | |
| CN110361442A (en) | A kind of excretion body and the preparation method and application thereof for mass spectrum flow cytomery | |
| CN109752540A (en) | A kind of circulating tumor cell parting quantitative test card and preparation method thereof and application method | |
| CN109060640A (en) | CD series of cell for detecting bone-marrow-derived lymphocyte detects slide | |
| CN108152189A (en) | A kind of sperm surface goes out the quantitative detecting method of cloud albumen 1 | |
| CN106996975B (en) | Circulating tumor cell albumen parting kit | |
| Okada | Tissue-specificity in the soluble antigens in kidney microsomes | |
| CN206193008U (en) | Micro -fluidic device and reagent card | |
| Xu et al. | Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk | |
| CN109781975A (en) | The reagent and method of enrichment cycles rare cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180629 |