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CN108165421A - A kind of preparation method with the sealwort adlay health liquor for enhancing immune function - Google Patents

A kind of preparation method with the sealwort adlay health liquor for enhancing immune function Download PDF

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CN108165421A
CN108165421A CN201810229050.5A CN201810229050A CN108165421A CN 108165421 A CN108165421 A CN 108165421A CN 201810229050 A CN201810229050 A CN 201810229050A CN 108165421 A CN108165421 A CN 108165421A
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polygonatum
sealwort
rhizoma
powder
mixture
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马赞华
张伟国
钱和
马羚翔
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Jiangsu Fu Shou Bio Technology Co Ltd
Jiangnan University
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Jiangsu Fu Shou Bio Technology Co Ltd
Jiangnan University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/896Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8969Polygonatum (Solomon's seal)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/899Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
    • A61K36/8994Coix (Job's tears)

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Abstract

The invention discloses a kind of preparation methods for the sealwort Yiren wine health liquor for having and enhancing immune function, belong to brewing technical field.The present invention handles sealwort, adlay both integration of drinking and medicinal herbs materials using the methods of crushings, boiling, extraction, enzymatic treatment, access strain ferments after control material-water ratio, is squeezed, filtered after fermentation, blending a kind of health liquor full of nutrition, having enhancing immune function of acquisition.The present invention proposes the preparation method of a kind of energy simplification of flowsheet, the sealwort adlay health liquor for improving raw material availability, and sealwort adlay health liquor obtained has invigorating the spleen to clear away damp pathogen, invigorating the lung and the kidney, the health-care efficacy for improving immunity etc..

Description

一种具有增强免疫功能的黄精薏仁保健酒的制备方法Preparation method of sealwort barley health wine with enhanced immune function

技术领域technical field

本发明涉及一种具有增强免疫功能的黄精薏仁保健酒的制备方法,属于酿酒技术领域。The invention relates to a preparation method of Polygonatum Coix Seed health wine with enhanced immune function, which belongs to the technical field of wine making.

背景技术Background technique

当代人对于养生保健的重视程度已经越来越高,不只是老年人,许多年轻人都开始追求养生保健,并且热衷于购买养生保健食品,保健酒就是其中比较热销的一种。Contemporary people have paid more and more attention to health care. Not only the elderly, but also many young people have begun to pursue health care and are keen to buy health care food. Health wine is one of the most popular products.

黄精是我国传统的药食同源的中药材,味甘、平,具有补血益肾、降血糖血脂、抗肿瘤、免疫调节等作用。《本草纲目》记载“补气益肾,除风湿,安五脏;久服轻身延年不饥;补五劳七伤,助筋骨、耐寒暑、润心肺”,黄精具有很高的药用价值。黄精的主要成分有多糖、皂苷、蒽醌、生物碱等,有降血糖、抗疲劳、抗氧化和延缓衰老的作用,在医疗养生保健等领域都具有很高的地位。薏仁也是国家认证的药食同源植物,被誉为“世界禾本科植物之王”,中医认为其味甘,性凉,具有健脾祛湿、补肺清热、养颜驻容的功效。薏仁的营养非常丰富,主要的功能性成分有薏仁油、薏仁酯、薏仁多糖等,具有抗肿瘤、抗癌、调节血糖血脂、提高免疫力等功效,在医疗养生保健等领域也具有很重要的地位。Polygonatum is a traditional Chinese medicinal material with the same origin as medicine and food in my country. It tastes sweet and flat, and has the functions of nourishing blood and kidney, lowering blood sugar and blood fat, anti-tumor, and immune regulation. "Compendium of Materia Medica" records that "invigorate qi and kidney, remove rheumatism, and calm the five internal organs; take it for a long time to lighten the body and prolong life without hunger; tonify five labors and seven injuries, help muscles and bones, resist cold and heat, and moisten the heart and lungs." Polygonatum has high medicinal value. The main components of sealwort are polysaccharides, saponins, anthraquinones, alkaloids, etc., which have the functions of lowering blood sugar, anti-fatigue, anti-oxidation and delaying aging, and have a high status in the fields of medical care and health care. Barley is also a nationally certified medicinal and edible plant. It is known as the "king of grasses in the world". Traditional Chinese medicine believes that it is sweet in taste and cool in nature. Barley is very nutritious. The main functional ingredients are barley oil, barley esters, barley polysaccharides, etc., which have anti-tumor, anti-cancer, blood sugar and blood lipid regulation, and improved immunity. status.

因此,将黄精、薏仁作为原料制备黄精薏仁保健酒,不仅能达到良好的保健效果,也具有很大的市场。Therefore, using sealwort and barley as raw materials to prepare sealwort and barley health wine can not only achieve good health care effect, but also has a large market.

目前,已有公开号为CN 103642648A的发明专利:一种薏仁酒及其制作方法,公开了一种利用薏仁、香禾糯米、高粱和稻壳为原料,加入烧酒曲糖化发酵再进行蒸馏后得到的45度酒,该方案得到的45度酒存在辣口、上头的问题,作为一种保健酒却不适合大部分有需要的人群饮用,若加水勾兑降低其酒精度,又会对其风味造成很大的破坏,难以入口;也有公开号为CN 104130906A的发明专利:一种黄精糯米酒配方及其酿制方法,其采用黄精、玉竹为主药,利用糯米多次加饭加药液技术酿制该保健酒,但该方案需事先准备好黄精玉竹提取液和煎煮药液,在发酵过程中多次喂饭,工艺较为繁琐,其中黄精玉竹提取液是通过榨汁获取滤液,会产生大量残渣,造成原料的浪费。At present, there is an invention patent with the publication number CN 103642648A: a barley wine and its production method, which discloses a barley wine, glutinous rice, sorghum and rice husk as raw materials, adding shochu koji, saccharifying and fermenting, and then distilling. 45% wine, the 45% wine obtained by this scheme has the problem of spicy taste and topping. As a health wine, it is not suitable for most people in need. If water is added to reduce its alcohol content, it will affect its flavor It is very destructive and difficult to import; there is also an invention patent with a publication number of CN 104130906A: a formula of Polygonatum glutinous rice wine and its brewing method, which uses Polygonatum and Polygonatum as the main ingredients, and utilizes the technology of adding glutinous rice multiple times to add rice and liquid medicine Brew the health wine, but this plan needs to prepare Polygonatum Polygonatum Extract and decocting liquid in advance, and feed rice many times during the fermentation process, the process is relatively cumbersome, and the Polygonatum Polygonatum Extract is obtained by squeezing the juice. Can produce a large amount of residue, cause the waste of raw material.

可以说,现有方法均存在步骤繁琐,或者有益成分提取效果不佳、原料利用率不高等的缺陷,因此,有必要开发一种省时高效、原料利用率高、有益成分含量高的黄精薏仁保健酒的制备方法。It can be said that the existing methods all have the defects of cumbersome steps, poor extraction effect of beneficial ingredients, and low utilization rate of raw materials. The preparation method of health wine.

发明内容Contents of the invention

为了解决上述问题,本发明提供了一种具有增强免疫力功能的黄精薏仁保健酒的制备方法。本方法首先提取黄精中的有效成分,之后与薏仁一起进行酶制剂的处理,缩短工艺时间的同时提高了原料的利用率;在后续发酵过程中,添加糖液以控制酒精度,增强了黄精、薏仁非糖成分的利用率,同时让整个工艺流程易于控制,提高产品的稳定性。In order to solve the above problems, the present invention provides a preparation method of Polygonatum Polygonatum and Coix Seed Health Wine with the function of enhancing immunity. The method firstly extracts the active ingredients in Rhizoma Polygonatum, and then carries out enzyme preparation treatment together with Coix Seed, which shortens the process time and improves the utilization rate of raw materials; The utilization rate of the non-sugar ingredients of Job’s tears also makes the whole process easy to control and improves the stability of the product.

本发明的目的是提供一种具有增强免疫力功能的黄精薏仁保健酒的制备方法,本发明的技术方案为采用粉碎、纤维素酶浸提处理黄精得到黄精提取液混合物,采用粉碎处理薏仁得到薏仁粉,将处理好的黄精提取液混合物与薏仁粉按比例混合得到黄精薏仁混合液,采用蒸煮、液化酶液化、糖化酶糖化处理黄精薏仁混合液得到黄精薏仁糖化液,然后将得到的黄精薏仁糖化液接入菌种发酵,经过发酵的醪液经压榨过滤、澄清或精滤、勾兑得到黄精薏仁保健酒。The object of the present invention is to provide a preparation method of Rhizoma Polygonatum and Coix Seed Health Wine with the function of enhancing immunity. The technical scheme of the present invention is to use crushing and cellulase extraction to process Polygonatum Rhizoma Polygonatum Extract to obtain a mixture of Rhizoma Polygonatum Extract, and to process Coix Seed by crushing to obtain Coix Seed Powder, mix the treated Polygonatum extract mixture with coix seed powder in proportion to obtain Polygonatum coix seed mixed liquid, use steaming, liquefaction enzyme liquefaction, and glucoamylase saccharification to treat Polygonatum coix seed mixed liquid to obtain Polygonatum barley saccharification liquid, and then obtain Polygonatum barley saccharification solution The fermented liquid is fermented with strains, and the fermented mash is squeezed, filtered, clarified or finely filtered, and blended to obtain the sealwort barley health wine.

在本发明的一种实施方式中,采用的制备原料为黄精与薏仁;所述黄精根茎要求冲洗干净;所述薏仁要求颗粒饱满、无霉变粒;所述黄精先切块,再用粉碎机粉碎成40-60目的粉粒;所述薏仁用粉碎机粉碎成40-60目的粉粒。In one embodiment of the present invention, the raw materials used for preparation are sealwort and barley; the rhizome of sealwort requires to be rinsed clean; the grains of barley are required to be full and free of mildew; the sealwort is first cut into pieces and then crushed with a pulverizer. Pulverized into 40-60 mesh powder; the coix seed is crushed into 40-60 mesh powder with a pulverizer.

在本发明的一种实施方式中,所述纤维素酶浸提是将黄精粉与水按比例混合,然后加入50-100U/g的纤维素酶,在45-55℃的条件下处理60-80min,获得黄精提取液混合物。In one embodiment of the present invention, the cellulase extraction is to mix Polygonatum powder and water in proportion, then add 50-100U/g cellulase, and treat 60- 80min, obtain the sealwort extract mixture.

在本发明的一种实施方式中,所述纤维素酶浸提是将黄精粉与水按质量比1:4-1:6的比例混合。In one embodiment of the present invention, the cellulase extraction is to mix Polygonatum powder and water in a mass ratio of 1:4-1:6.

在本发明的一种实施方式中,所述纤维素酶浸提是将黄精粉与水按质量比1:5的比例混合。In one embodiment of the present invention, the cellulase extraction is to mix Polygonatum powder and water in a mass ratio of 1:5.

在本发明的一种实施方式中,所述方法中黄精提取液混合物与薏仁粉是按比例混合;所述蒸煮的时间为15-20min;所述液化酶液化是加入80-120U/g的液化酶在80-95℃的条件下液化60-80min;所述糖化酶糖化是加入90-150U/g糖化酶在58-65℃的温度下糖化80-120min。In one embodiment of the present invention, in the method, the Polygonatum extract mixture and the barley powder are mixed in proportion; the cooking time is 15-20min; The enzyme is liquefied under the condition of 80-95° C. for 60-80 minutes; the saccharifying enzyme is saccharified by adding 90-150 U/g of glucoamylase and saccharifying at the temperature of 58-65° C. for 80-120 minutes.

在本发明的一种实施方式中,所述方法中黄精提取液混合物与薏仁粉是按质量比1:1的比例混合。In one embodiment of the present invention, in the method, the mixture of Rhizoma Polygonatum extract and barley powder is mixed in a mass ratio of 1:1.

在本发明的一种实施方式中,所述菌种包括米根霉和活性干酵母;所述活性干酵母包括酿酒酵母和生香酵母。In one embodiment of the present invention, the strains include Rhizopus oryzae and active dry yeast; the active dry yeast includes Saccharomyces cerevisiae and Saccharomyces cerevisiae.

在本发明的一种实施方式中,所述米根霉使用菌丝接种;所述酿酒酵母接种量为0.2-0.4%;所述生香酵母接种量为0.3-0.6%。In one embodiment of the present invention, the Rhizopus oryzae is inoculated with mycelia; the inoculation amount of the Saccharomyces cerevisiae is 0.2-0.4%; the inoculation amount of the Aroma yeast is 0.3-0.6%.

在本发明的一种实施方式中,所述发酵为在30℃的条件下发酵培养,经过12-14h进行头耙,降低品温,之后按照发酵进程加入糖液并开耙一次;7-9d后,品温与室温相接近,此时发酵阶段结束。In one embodiment of the present invention, the fermentation is carried out under the condition of 30° C., after 12-14 hours of head raking, the product temperature is lowered, and then the sugar solution is added according to the fermentation process and raked once; 7-9d Finally, the product temperature is close to room temperature, and the fermentation stage ends at this moment.

在本发明的一种实施方式中,所述方法包括以下步骤:In one embodiment of the present invention, described method comprises the following steps:

步骤一,将黄精粉碎成黄精粉后加入水,搅拌均匀,加入50-100U/g的纤维素酶,在45-55℃的条件下提取60-80min,获得黄精提取液混合物;将薏仁粉碎成薏仁粉;将黄精提取液混合物与薏仁粉按比例进行混合得到黄精薏仁混合液;Step 1: crush Rhizoma Polygonatum into Rhizoma Rhizoma Polygonatum powder, add water, stir evenly, add 50-100U/g cellulase, extract at 45-55°C for 60-80min to obtain a mixture of Rhizoma Rhizoma Rhizoma Polygonatum extract; grind barley into Coix seed powder; mixing the sealwort extract mixture with the job's tears powder in proportion to obtain the Rhizoma Polygonatum and Coix seed mixed solution;

步骤二,将黄精薏仁混合液蒸煮15-20min;之后调节混合液温度至80-95℃,用碱/酸液调节pH为5.5-6.0,加入80-120U/g的液化酶在80-95℃的条件下液化60-80min;再将液化后的混合液温度降低为58-65℃,加入90-150U/g糖化酶在58-65℃下糖化80-120min,最终获得黄精薏仁糖化液;Step 2: Cook the mixture of sealwort and barley for 15-20 minutes; then adjust the temperature of the mixture to 80-95°C, adjust the pH to 5.5-6.0 with alkali/acid solution, add 80-120U/g liquefaction enzyme at 80-95°C Liquefaction under the condition of 60-80min; then reduce the temperature of the liquefied mixed solution to 58-65℃, add 90-150U/g glucoamylase and saccharify at 58-65℃ for 80-120min, and finally obtain the saccharification solution of sealwort barley;

步骤三,将活性干酵母在含有2%糖的水溶液37℃条件下活化20-30min;Step 3, activating the active dry yeast in an aqueous solution containing 2% sugar at 37°C for 20-30min;

步骤三,在黄精薏仁糖化液品温降低至26-30℃时接种活性干酵母和米根霉,酿酒酵母接种量为0.2-0.4%,生香酵母接种量为0.3-0.6%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过12-14h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;7-9d后,品温与室温相接近,此时发酵阶段结束;Step 3: Inoculate active dry yeast and Rhizopus oryzae when the product temperature of Polygonatum Coix seed saccharified liquid drops to 26-30°C, the inoculum amount of Saccharomyces cerevisiae is 0.2-0.4%, the inoculum amount of Aroma Yeast is 0.3-0.6%, and Rhizopus oryzae is inoculated Use mycelium to inoculate, pre-ferment and cultivate under the condition of 30°C, carry out head rake after 12-14h, lower the product temperature, add sugar solution according to the fermentation process and open rake once in the subsequent fermentation process, the purpose is to reduce the product temperature and make the fermentation Carry out evenly; After 7-9 days, the product temperature is close to room temperature, and the fermentation stage is over at this time;

步骤四,将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,90-95℃灭菌后进行勾兑即为成品酒。Step 4: Squeeze and filter the fermented mash to obtain filtrate, set it aside for clarification at low temperature or fine filter with diatomaceous earth, sterilize at 90-95°C and blend to obtain finished wine.

在本发明的一种实施方式中,所述步骤一中黄精粉与水的质量比为1:4-1:6。In one embodiment of the present invention, the mass ratio of Rhizoma Polygonati powder to water in the step 1 is 1:4-1:6.

在本发明的一种实施方式中,所述步骤一中黄精粉与水的质量比为1:5。In one embodiment of the present invention, the mass ratio of Rhizoma Polygonati powder to water in the step 1 is 1:5.

在本发明的一种实施方式中,所述方法中黄精提取液混合物与薏仁粉是按质量比1:1的比例混合。In one embodiment of the present invention, in the method, the mixture of Rhizoma Polygonatum extract and barley powder is mixed in a mass ratio of 1:1.

本发明提供了一种根据上述方法制备得到的黄精薏仁保健酒。The invention provides a sealwort barley health wine prepared according to the above method.

有益效果:Beneficial effect:

(1)本发明得到的这种保健酒具有有降血糖、抗疲劳、抗氧化和延缓衰老的作用等的功效,薏仁具有抗肿瘤、抗癌、调节血糖血脂、提高免疫力等的功效,以两者为原料制备的保健酒具有很强的健脾祛湿、补肺益肾、增强免疫力的保健功能。(1) The health wine obtained by the present invention has the effects of lowering blood sugar, anti-fatigue, anti-oxidation and delaying senility, etc. Barley has the effects of anti-tumor, anti-cancer, regulating blood sugar and blood fat, improving immunity, etc. The health-care wine prepared from the two materials has strong health-care functions of invigorating the spleen and eliminating dampness, nourishing the lungs and kidneys, and enhancing immunity.

(2)本发明使用纤维素酶浸提预处理黄精原料,提高了原料利用率,使用优化后的纤维素酶处理条件可以提高黄精中皂苷和多糖的提取率,皂苷提取率可达34.22%,多糖提取率可达10.24%,高于水浴提取法;本发明在浸提后与薏仁混合进行酶处理,进一步提高了原料利用率,黄精中的有效物质皂苷与多糖分别提高了54.3%、68.6%,并且通过优化黄精提取和酶处理工艺参数缩短了20-30%的工艺时间。(2) the present invention uses cellulase extraction pretreatment Polygonatum raw material, has improved raw material utilization rate, uses the optimized cellulase treatment condition and can improve the extraction rate of saponin and polysaccharide in Polygonatum, saponin extraction rate can reach 34.22%, The polysaccharide extraction rate can reach 10.24%, which is higher than that of the water bath extraction method; the invention mixes the barley with coix seed for enzyme treatment after extraction, which further improves the utilization rate of raw materials, and the effective substance saponin and polysaccharide in Polygonatum are increased by 54.3% and 68.6% respectively , and shortened the process time by 20-30% by optimizing the process parameters of Polygonatum extract and enzyme treatment.

(3)本发明的糖化液化过程主要是将原料中的淀粉分解为酵母能够利用的葡萄糖,使用优化后的糖化液化工艺可以获得较好的糖化液化效果,DE值达到78.57%。(3) The saccharification and liquefaction process of the present invention mainly decomposes the starch in the raw material into glucose that can be used by the yeast. The optimized saccharification and liquefaction process can obtain a better saccharification and liquefaction effect, and the DE value reaches 78.57%.

(4)本发明在发酵过程中通过添加糖液方式控制发酵时间和酒精度,此方法可以控制酒精度在适宜范围内,提高了产品批次间质量的稳定性。(4) The present invention controls the fermentation time and the alcohol content by adding sugar solution in the fermentation process. This method can control the alcohol content within a suitable range and improves the stability of product batch quality.

(5)本发明利用酵母和米根霉混菌发酵,可以增强发酵效果,同时给保健酒增加了独特的风味。(5) The present invention utilizes yeast and Rhizopus oryzae to ferment mixed bacteria, which can enhance the fermentation effect and add unique local flavor to the health care wine at the same time.

(6)本发明使用的黄精、薏仁本身具有味甘的特性,可很大程度上缓解酒精辣口,难以入喉的问题,采用黄精、薏仁制备出的保健酒口味适中,适合大部分人群引用。(6) Rhizoma Polygonatum and Job's tears used in the present invention have the characteristics of sweet taste, which can largely alleviate the problem of alcohol being spicy and difficult to enter the throat. .

附图说明Description of drawings

图1为本发明的黄精薏仁保健酒的制备工艺流程示意图。Fig. 1 is the schematic flow chart of the preparation process of Polygonatum Polygonatum and Job's tears health wine of the present invention.

具体实施方式Detailed ways

下面结合实施例与对比例对本发明进行具体描述。The present invention will be specifically described below in conjunction with examples and comparative examples.

实施例1Example 1

实施例1采用的制备方法如下:The preparation method that embodiment 1 adopts is as follows:

(1)将黄精粉碎成粉后加入5倍的水,搅拌均匀,加入100U/g的纤维素酶,在52℃的条件下提取80min,获得黄精提取液混合物;按质量比1:1加入薏仁粉,蒸煮20min,之后调节混合物料温度至85℃,用碱/酸液调节pH为5.5,加入120U/g的液化酶在95℃的条件下液化80min;下一步将温度降低为58℃,加入糖化酶,用量为120U/g,处理时间为100min,最终获得黄精薏仁糖化液;(1) Grind Polygonatum into powder, add 5 times of water, stir evenly, add 100U/g cellulase, extract at 52°C for 80min to obtain the mixture of Polygonatum extract; add barley at a mass ratio of 1:1 Powder, cook for 20min, then adjust the temperature of the mixture to 85°C, adjust the pH to 5.5 with alkali/acid solution, add 120U/g liquefying enzyme to liquefy at 95°C for 80min; in the next step, reduce the temperature to 58°C, add Glucoamylase, the dosage is 120U/g, and the treatment time is 100min, finally obtain the saccharification solution of sealwort barley;

(2)活性干酵母在含有2%糖的水溶液37℃条件下活化30min;(2) Active dry yeast is activated for 30 minutes at 37° C. in an aqueous solution containing 2% sugar;

(3)物料品温降低至30℃时接种酵母和米根霉,酿酒酵母接种量为0.4%,生香酵母接种量为0.6%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过14h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;9d后,品温与室温相接近,此时发酵阶段结束;(3) Inoculate yeast and Rhizopus oryzae when the product temperature of the material is reduced to 30°C, the inoculation amount of Saccharomyces cerevisiae is 0.4%, the inoculum amount of Aroma Yeast is 0.6%, and Rhizopus oryzae is inoculated with mycelia, and the Fermentation culture, after 14 hours of raking, the product temperature was lowered. In the subsequent fermentation process, sugar solution was added and raked once according to the fermentation process. The purpose was to reduce the product temperature and make the fermentation evenly carried out; When the fermentation stage ends;

(4)将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,90℃灭菌后进行勾兑即为成品酒;(4) Squeeze and filter the fermented mash to obtain the filtrate, set it aside for clarification at low temperature or finely filter it with diatomaceous earth, sterilize at 90°C and blend to obtain the finished wine;

(5)成品酒酒精度达到13.47度,酸度3.5g/L,多糖15.86g/L,皂苷145mg/100mL,酒体呈金黄色,风味醇厚和谐。(5) The alcohol content of the finished wine reaches 13.47 degrees, the acidity is 3.5g/L, the polysaccharide is 15.86g/L, the saponin is 145mg/100mL, the wine body is golden yellow, and the flavor is mellow and harmonious.

实施例2Example 2

实施例2采用的制备方法如下:The preparation method that embodiment 2 adopts is as follows:

(1)将黄精粉碎成粉后加入4倍的水,搅拌均匀,加入80U/g的纤维素酶,在55℃的条件下提取60min,获得黄精提取液混合物;按质量比1:1加入薏仁粉,蒸煮20min,之后调节混合物料温度至90℃,用碱/酸液调节pH为5.5,加入100U/g的液化酶在90℃的条件下液化60min;下一步将温度降低为60℃,加入糖化酶,用量为120U/g,处理时间为90min,最终获得黄精薏仁糖化液;(1) Grind Rhizoma Polygonatum into powder, add 4 times of water, stir well, add 80U/g cellulase, extract at 55°C for 60min to obtain a mixture of Rhizoma Rhizoma Polygonatum extract; add barley at a mass ratio of 1:1 powder, steamed for 20min, then adjusted the temperature of the mixed material to 90°C, adjusted the pH to 5.5 with alkali/acid solution, added 100U/g liquefaction enzyme and liquefied at 90°C for 60min; in the next step, lowered the temperature to 60°C, added The dosage of glucoamylase is 120U/g, and the treatment time is 90min, finally obtain the saccharification solution of sealwort barley;

(2)活性干酵母在含有2%糖的水溶液37℃条件下活化20min;(2) Active dry yeast is activated for 20 minutes at 37° C. in an aqueous solution containing 2% sugar;

(3)物料品温降低至30℃时接种酵母和米根霉,酿酒酵母接种量为0.3%,生香酵母接种量为0.4%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过12h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;8d后,品温与室温相接近,此时发酵阶段结束;(3) Inoculate yeast and Rhizopus oryzae when the product temperature of the material is reduced to 30°C, the inoculation amount of Saccharomyces cerevisiae is 0.3%, the inoculum amount of Aroma Yeast is 0.4%, and Rhizopus oryzae is inoculated with mycelium, and the inoculation amount of Rhizopus oryzae is 30°C. Fermentation culture, after 12 hours of raking, lowering the product temperature, adding sugar solution according to the fermentation process and raking once in the subsequent fermentation process, the purpose is to reduce the product temperature and make the fermentation evenly carried out; after 8 days, the product temperature is close to room temperature, this time When the fermentation stage ends;

(4)将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,95℃灭菌后进行勾兑即为成品酒;(4) Squeeze and filter the fermented mash to obtain the filtrate, set it aside for clarification at low temperature or fine filter with diatomaceous earth, sterilize at 95°C and blend to obtain the finished wine;

(5)成品酒酒精度达到12.97度,酸度3.9g/L,多糖16.26g/L,皂苷136mg/100mL,酒体呈金黄色,风味醇厚和谐。(5) The alcohol content of the finished wine reaches 12.97 degrees, the acidity is 3.9g/L, the polysaccharide is 16.26g/L, the saponin is 136mg/100mL, the wine body is golden yellow, and the flavor is mellow and harmonious.

实施例3Example 3

实施例3采用的制备方法如下:The preparation method that embodiment 3 adopts is as follows:

(1)将黄精粉碎成粉后加入5倍的水,搅拌均匀,加入50U/g的纤维素酶,在45℃的条件下提取75min,获得黄精提取液混合物;按质量比1:1加入薏仁粉,蒸煮15min,之后调节混合物料温度至95℃,用碱/酸液调节pH为6.0,加入80U/g的液化酶在95℃的条件下液化70min;下一步将温度降低为65℃,加入糖化酶,用量为150U/g,处理时间为80min,最终获得黄精薏仁糖化液;(1) Grind Rhizoma Polygonatum into powder, add 5 times of water, stir well, add 50U/g cellulase, extract at 45°C for 75min to obtain a mixture of Rhizoma Rhizoma Polygonatum extract; add barley at a mass ratio of 1:1 Powder, cook for 15 minutes, then adjust the temperature of the mixture to 95°C, adjust the pH to 6.0 with alkali/acid solution, add 80U/g liquefying enzyme and liquefy at 95°C for 70min; The dosage of glucoamylase is 150U/g, and the treatment time is 80min, finally obtain the saccharification solution of sealwort barley;

(2)活性干酵母在含有2%糖的水溶液37℃条件下活化30min;(2) Active dry yeast is activated for 30 minutes at 37° C. in an aqueous solution containing 2% sugar;

(3)物料品温降低至26℃时接种酵母和米根霉,酿酒酵母接种量为0.2%,生香酵母接种量为0.3%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过13h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;9d后,品温与室温相接近,此时发酵阶段结束;(3) Inoculate yeast and Rhizopus oryzae when the product temperature of the material is reduced to 26°C, the inoculation amount of Saccharomyces cerevisiae is 0.2%, the inoculum amount of Aroma Yeast is 0.3%, and Rhizopus oryzae is inoculated with mycelium, and the inoculation amount of Rhizopus oryzae is 30°C. Fermentation culture, after 13 hours of raking, lowering the product temperature, adding sugar solution according to the fermentation process and raking once in the subsequent fermentation process, the purpose is to reduce the product temperature and make the fermentation evenly; after 9 days, the product temperature is close to room temperature, and the When the fermentation stage ends;

(4)将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,90℃灭菌后进行勾兑即为成品酒;(4) Squeeze and filter the fermented mash to obtain the filtrate, set it aside for clarification at low temperature or finely filter it with diatomaceous earth, sterilize at 90°C and blend to obtain the finished wine;

(5)成品酒酒精度达到13.41度,酸度3.7g/L,多糖13.76g/L,皂苷152mg/100mL,酒体呈金黄色,风味醇厚和谐。(5) The alcohol content of the finished wine reaches 13.41 degrees, the acidity is 3.7g/L, the polysaccharide is 13.76g/L, the saponin is 152mg/100mL, the wine body is golden yellow, and the flavor is mellow and harmonious.

对比例1Comparative example 1

不使用纤维素酶预先处理黄精,直接采用蒸煮提取发酵原材料。The sealwort is not pre-treated with cellulase, and the fermentation raw material is directly extracted by cooking.

(1)将黄精粉碎成粉后加入4倍的水,搅拌均匀,在60℃的条件下浸提60min,获得黄精提取液混合物;按质量比1:1加入薏仁粉,蒸煮20min,之后调节混合物料温度至90℃,用碱/酸液调节pH为5.5,加入100U/g的液化酶在90℃的条件下液化60min;下一步将温度降低为60℃,加入糖化酶,用量为120U/g,处理时间为90min,最终获得黄精薏仁糖化液。(1) Crush Rhizoma Polygonatum into powder, add 4 times of water, stir evenly, and extract at 60°C for 60 minutes to obtain a mixture of Rhizoma Rhizoma Polygonatum extract; add barley powder at a mass ratio of 1:1, cook for 20 minutes, and then adjust the mixture The temperature of the material is 90°C, adjust the pH to 5.5 with alkali/acid solution, add 100U/g of liquefying enzyme to liquefy at 90°C for 60 minutes; in the next step, lower the temperature to 60°C, add glucoamylase at a dosage of 120U/g , and the processing time was 90min, and finally obtained the saccharified liquid of sealwort and barley.

(2)活性干酵母在含有2%糖的水溶液37℃条件下活化20min;(2) Active dry yeast is activated for 20 minutes at 37° C. in an aqueous solution containing 2% sugar;

(3)物料品温降低至30℃时接种酵母和米根霉,酿酒酵母接种量为0.3%,生香酵母接种量为0.4%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过12h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;8d后,品温与室温相接近,此时发酵阶段结束;(3) Inoculate yeast and Rhizopus oryzae when the product temperature of the material is reduced to 30°C, the inoculation amount of Saccharomyces cerevisiae is 0.3%, the inoculum amount of Aroma Yeast is 0.4%, and Rhizopus oryzae is inoculated with mycelium, and the inoculation amount of Rhizopus oryzae is 30°C. Fermentation culture, after 12 hours of raking, lowering the product temperature, adding sugar solution according to the fermentation process and raking once in the subsequent fermentation process, the purpose is to reduce the product temperature and make the fermentation evenly carried out; after 8 days, the product temperature is close to room temperature, this time When the fermentation stage ends;

(4)将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,95℃灭菌后进行勾兑即为成品酒;(4) Squeeze and filter the fermented mash to obtain the filtrate, set it aside for clarification at low temperature or fine filter with diatomaceous earth, sterilize at 95°C and blend to obtain the finished wine;

(5)制得的成品酒酒精度达到12.54%,酸度3.1g/L,多糖10.35g/L,皂苷85mg/100mL,黄精营养物质含量显著降低。(5) The alcohol content of the finished wine reached 12.54%, the acidity was 3.1g/L, the polysaccharide was 10.35g/L, the saponin was 85mg/100mL, and the nutrient substance content of Polygonatum was significantly reduced.

对比例2Comparative example 2

对比例2采用的制备方法如下:改变黄精提取工艺参数The preparation method that comparative example 2 adopts is as follows: Change Polygonatum Extraction Process Parameters

(1)将黄精粉碎成粉后加入4倍的水,搅拌均匀,加入180U/g的纤维素酶,在65℃的条件下提取90min,获得黄精提取液混合物;按质量比1:1加入薏仁粉,蒸煮15min,之后调节混合物料温度至95℃,用碱/酸液调节pH为6.0,加入80U/g的液化酶在95℃的条件下液化70min;下一步将温度降低为65℃,加入糖化酶,用量为150U/g,处理时间为80min,最终获得黄精薏仁糖化液;(1) Grind Rhizoma Polygonatum into powder, add 4 times of water, stir well, add 180U/g cellulase, extract at 65°C for 90min to obtain a mixture of Rhizoma Rhizoma Polygonatum extract; add barley at a mass ratio of 1:1 Powder, cook for 15 minutes, then adjust the temperature of the mixture to 95°C, adjust the pH to 6.0 with alkali/acid solution, add 80U/g liquefying enzyme and liquefy at 95°C for 70min; The dosage of glucoamylase is 150U/g, and the treatment time is 80min, finally obtain the saccharification solution of sealwort barley;

(2)活性干酵母在含有2%糖的水溶液37℃条件下活化30min;(2) Active dry yeast is activated for 30 minutes at 37° C. in an aqueous solution containing 2% sugar;

(3)物料品温降低至30℃时接种酵母和米根霉,酿酒酵母接种量为0.2%,生香酵母接种量为0.4%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过12h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;9d后,品温与室温相接近,此时发酵阶段结束;(3) Inoculate yeast and Rhizopus oryzae when the product temperature of the material is reduced to 30°C, the inoculation amount of Saccharomyces cerevisiae is 0.2%, the inoculum amount of Aroma Yeast is 0.4%, and Rhizopus oryzae is inoculated with mycelium, and the inoculation amount of Rhizopus oryzae is 30°C. Fermentation culture, after 12 hours of raking, the product temperature was lowered. In the subsequent fermentation process, sugar solution was added and raked once according to the fermentation process. The purpose was to reduce the product temperature and make the fermentation evenly carried out; When the fermentation stage ends;

(4)将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,90℃灭菌后进行勾兑即为成品酒;(4) Squeeze and filter the fermented mash to obtain the filtrate, set it aside for clarification at low temperature or finely filter it with diatomaceous earth, sterilize at 90°C and blend to obtain the finished wine;

(5)成品酒酒精度达到13.24度,酸度4.1g/L,多糖7.39g/L,皂苷93mg/100mL,皂苷含量较低,降低了保健价值。(5) The alcohol content of the finished wine reaches 13.24 degrees, the acidity is 4.1g/L, the polysaccharide is 7.39g/L, and the saponin is 93mg/100mL. The saponin content is low, which reduces the health care value.

对比例3Comparative example 3

对比例3采用的制备方法如下:改变发酵条件The preparation method that comparative example 3 adopts is as follows: change fermentation condition

(1)将黄精粉碎成粉后加入5倍的水,搅拌均匀,加入80U/g的纤维素酶,在52℃的条件下提取80min,获得黄精提取液混合物;按质量比1:1加入薏仁粉,蒸煮18min,之后调节混合物料温度至92℃,用碱/酸液调节pH为5.8,加入120U/g的液化酶在95℃的条件下液化80min;下一步将温度降低为60℃,加入糖化酶,用量为120U/g,处理时间为90min,最终获得黄精薏仁糖化液;(1) Grind Polygonatum into powder, add 5 times of water, stir evenly, add 80U/g cellulase, extract at 52°C for 80min to obtain the mixture of Polygonatum extract; add barley at a mass ratio of 1:1 Powder, steamed for 18 minutes, then adjusted the temperature of the mixed material to 92°C, adjusted the pH to 5.8 with alkali/acid solution, added 120U/g liquefaction enzyme and liquefied at 95°C for 80min; in the next step, lower the temperature to 60°C, add The dosage of glucoamylase is 120U/g, and the treatment time is 90min, finally obtain the saccharification solution of sealwort barley;

(2)活性干酵母在含有2%糖的水溶液37℃条件下活化30min;(2) Active dry yeast is activated for 30 minutes at 37° C. in an aqueous solution containing 2% sugar;

(3)物料品温降低至35℃时接种酵母和米根霉,酿酒酵母接种量为0.5%,生香酵母接种量为1.0%,米根霉使用菌丝接种,在35℃的条件下前发酵培养,经过12h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;8d后,品温与室温相接近,此时发酵阶段结束;(3) Inoculate yeast and Rhizopus oryzae when the product temperature of the material is reduced to 35°C, the inoculum amount of Saccharomyces cerevisiae is 0.5%, the inoculum amount of Aroma Yeast is 1.0%, and Rhizopus oryzae is inoculated with mycelia. Fermentation culture, after 12 hours of raking, lowering the product temperature, adding sugar solution according to the fermentation process and raking once in the subsequent fermentation process, the purpose is to reduce the product temperature and make the fermentation evenly carried out; after 8 days, the product temperature is close to room temperature, this time When the fermentation stage ends;

(4)将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,95℃灭菌后进行勾兑即为成品酒;(4) Squeeze and filter the fermented mash to obtain the filtrate, set it aside for clarification at low temperature or fine filter with diatomaceous earth, sterilize at 95°C and blend to obtain the finished wine;

(5)成品酒酒精度达到9.43度,酸度16.8g/L,多糖6.63g/L,皂苷141mg/100mL,酒体呈金黄色,酒度较低,酸度较高,酒风味不和谐。(5) The alcohol content of the finished wine reaches 9.43 degrees, the acidity is 16.8g/L, the polysaccharide is 6.63g/L, and the saponin is 141mg/100mL.

对比例4Comparative example 4

对比例4采用的制备方法如下:在发酵进程中没有补充糖液The preparation method that comparative example 4 adopts is as follows: do not supplement sugar solution in fermentation process

(1)将黄精粉碎成粉后加入5倍的水,搅拌均匀,加入50U/g的纤维素酶,在45℃的条件下提取75min,获得黄精提取液混合物;按质量比1:1加入薏仁粉,蒸煮15min,之后调节混合物料温度至95℃,用碱/酸液调节pH为5.8,加入80U/g的液化酶在95℃的条件下液化70min;下一步将温度降低为65℃,加入糖化酶,用量为150U/g,处理时间为80min,最终获得黄精薏仁糖化液;(1) Grind Rhizoma Polygonatum into powder, add 5 times of water, stir well, add 50U/g cellulase, extract at 45°C for 75min to obtain a mixture of Rhizoma Rhizoma Polygonatum extract; add barley at a mass ratio of 1:1 Powder, cook for 15 minutes, then adjust the temperature of the mixture to 95°C, adjust the pH to 5.8 with alkali/acid solution, add 80U/g liquefying enzyme and liquefy at 95°C for 70min; The dosage of glucoamylase is 150U/g, and the treatment time is 80min, finally obtain the saccharification solution of sealwort barley;

(2)活性干酵母在含有2%糖的水溶液37℃条件下活化30min;(2) Active dry yeast is activated for 30 minutes at 37° C. in an aqueous solution containing 2% sugar;

(3)物料品温降低至26℃时接种酵母和米根霉,酿酒酵母接种量为0.2%,生香酵母接种量为0.3%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过12h进行头耙,降低品温,目的是降低品温和使发酵均匀进行;8d后,品温与室温相接近,此时发酵阶段结束;(3) Inoculate yeast and Rhizopus oryzae when the product temperature of the material is reduced to 26°C, the inoculation amount of Saccharomyces cerevisiae is 0.2%, the inoculum amount of Aroma Yeast is 0.3%, and Rhizopus oryzae is inoculated with mycelium, and the inoculation amount of Rhizopus oryzae is 30°C. Fermentation culture, after 12 hours of raking, lowering the product temperature, the purpose is to reduce the product temperature to make the fermentation evenly; after 8 days, the product temperature is close to room temperature, and the fermentation stage is over at this time;

(4)将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,90℃灭菌后进行勾兑即为成品酒;(4) Squeeze and filter the fermented mash to obtain the filtrate, set it aside for clarification at low temperature or finely filter it with diatomaceous earth, sterilize at 90°C and blend to obtain the finished wine;

(5)成品酒酒精度达到10.41度,酸度6.2g/L,多糖8.58g/L,皂苷149mg/100mL,酒精度和多糖含量偏低,风味不和谐。(5) The alcohol content of the finished wine reaches 10.41%, the acidity is 6.2g/L, the polysaccharide is 8.58g/L, the saponin is 149mg/100mL, the alcohol content and polysaccharide content are low, and the flavor is not harmonious.

虽然本发明已以较佳实施例公开如上,但其并非用以限定本发明,任何熟悉此技术的人,在不脱离本发明的精神和范围内,都可做各种的改动与修饰,因此本发明的保护范围应该以权利要求书所界定的为准。Although the present invention has been disclosed above with preferred embodiments, it is not intended to limit the present invention. Any person familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore The scope of protection of the present invention should be defined by the claims.

Claims (10)

1.一种具有增强免疫功能的黄精薏仁保健酒的制备方法,其特征在于,所述方法是采用粉碎、纤维素酶浸提处理黄精得到黄精提取液混合物,采用粉碎处理薏仁得到薏仁粉,将处理好的黄精提取液混合物与薏仁粉按比例混合得到黄精薏仁混合液,采用蒸煮、液化酶液化、糖化酶糖化处理黄精薏仁混合液得到黄精薏仁糖化液,然后将得到的黄精薏仁糖化液接入菌种发酵,经过发酵的醪液经压榨过滤、澄清或精滤、勾兑得到黄精薏仁保健酒。1. A preparation method of Polygonatum Polygonatum and Coix Seed health-care wine with enhanced immune function, characterized in that, the method is to adopt pulverization and cellulase extraction to process Polygonatum Polygonatum to obtain the Polygonatum Extract mixture, and to use pulverization to process Coix Seed to obtain Coix Seed Powder, and The treated sealwort extract mixture is mixed with the barley powder in proportion to obtain the sealwort adlay mixture, and the sealwort and adlay mixture is processed by cooking, liquefying enzyme liquefaction, and saccharification enzyme to obtain the sealwort saccharification solution, and then the obtained sealwort saccharification solution is added to Strains are fermented, and the fermented mash is squeezed, filtered, clarified or finely filtered, and blended to obtain the sealwort barley health wine. 2.根据权利要求1所述的方法,其特征在于,采用的制备原料为黄精与薏仁;所述黄精根茎要求冲洗干净;所述薏仁要求颗粒饱满、无霉变粒;所述黄精先切块,再用粉碎机粉碎成40-60目的粉粒;所述薏仁用粉碎机粉碎成40-60目的粉粒。2. the method according to claim 1 is characterized in that, the preparation raw material that adopts is Rhizoma Polygonatum and Seed of Job's tears; Said Rhizoma Rhizome of Rhizoma Rhizoma Polygonatum requires to be rinsed clean; Seed of Job's tears requires full granules and no moldy grains; , and then pulverized into 40-60 purpose powder with a pulverizer; the barley is pulverized into 40-60 purpose powder with a pulverizer. 3.根据权利要求1或2所述的方法,其特征在于,所述纤维素酶浸提是将黄精粉与水按比例混合,然后加入50-100U/g的纤维素酶,在45-55℃的条件下处理60-80min,获得黄精提取液混合物。3. according to the described method of claim 1 and 2, it is characterized in that, described cellulase leaching is that sealwort powder and water are mixed in proportion, then add the cellulase of 50-100U/g, at 45-55 Under the condition of ℃, treat for 60-80min to obtain the mixture of Polygonatum extract. 4.根据权利要求1-3任一所述的方法,其特征在于,所述纤维素酶浸提是将黄精粉与水按质量比1:4-1:6的比例混合。4. The method according to any one of claims 1-3, characterized in that, said cellulase extraction is mixing Polygonatum powder and water in a mass ratio of 1:4-1:6. 5.根据权利要求1-4任一所述的方法,其特征在于,所述方法中黄精提取液混合物与薏仁粉是按比例混合;所述蒸煮的时间为15-20min;所述液化酶液化是加入80-120U/g的液化酶在80-95℃的条件下液化60-80min;所述糖化酶糖化是加入90-150U/g糖化酶在58-65℃的温度下糖化80-120min。5. according to the method described in any one of claim 1-4, it is characterized in that, in the described method, Polygonatum extract mixture and Job's tears powder are mixed in proportion; The time of described steaming is 15-20min; The liquefaction enzyme liquefaction Adding 80-120U/g liquefaction enzyme to liquefy at 80-95°C for 60-80min; said saccharification with saccharification enzyme adding 90-150U/g saccharification enzyme at 58-65°C for 80-120min. 6.根据权利要求1-5任一所述的方法,其特征在于,所述菌种包括米根霉和活性干酵母;所述活性干酵母包括酿酒酵母和生香酵母。6. The method according to any one of claims 1-5, wherein the strains include Rhizopus oryzae and active dry yeast; and the active dry yeast includes Saccharomyces cerevisiae and Saccharomyces cerevisiae. 7.根据权利要求6所述的方法,其特征在于,所述米根霉使用菌丝接种;所述酿酒酵母接种量为0.2-0.4%;所述生香酵母接种量为0.3-0.6%。7. The method according to claim 6, characterized in that, the Rhizopus oryzae is inoculated with mycelia; the inoculation amount of the Saccharomyces cerevisiae is 0.2-0.4%; the inoculum amount of the aromatic yeast is 0.3-0.6%. 8.根据权利要求1-7任一所述的方法,其特征在于,所述发酵为在30℃的条件下发酵培养,经过12-14h进行头耙,降低品温,之后按照发酵进程加入糖液并开耙一次;7-9d后,品温与室温相接近,此时发酵阶段结束。8. The method according to any one of claims 1-7, characterized in that, the fermentation is fermented and cultivated at 30°C, after 12-14 hours of raking, the product temperature is lowered, and then sugar is added according to the fermentation process After 7-9 days, the product temperature is close to room temperature, and the fermentation stage is over at this time. 9.根据权利要求1-8任一所述的方法,其特征在于,包括如下步骤:9. The method according to any one of claims 1-8, comprising the steps of: 步骤一,将黄精粉碎成黄精粉后加入水,搅拌均匀,加入50-100U/g的纤维素酶,在45-55℃的条件下提取60-80min,获得黄精提取液混合物;将薏仁粉碎成薏仁粉;将黄精提取液混合物与薏仁粉按比例进行混合得到黄精薏仁混合液;Step 1: crush Rhizoma Polygonatum into Rhizoma Rhizoma Polygonatum powder, add water, stir evenly, add 50-100U/g cellulase, extract at 45-55°C for 60-80min to obtain a mixture of Rhizoma Rhizoma Rhizoma Polygonatum extract; grind barley into Coix seed powder; mixing the sealwort extract mixture with the job's tears powder in proportion to obtain the Rhizoma Polygonatum and Coix seed mixed solution; 步骤二,将黄精薏仁混合液蒸煮15-20min;之后调节混合液温度至80-95℃,用碱/酸液调节pH为5.5-6.0,加入80-120U/g的液化酶在80-95℃的条件下液化60-80min;再将液化后的混合液温度降低为58-65℃,加入90-150U/g糖化酶在58-65℃下糖化80-120min,最终获得黄精薏仁糖化液;Step 2: Cook the mixture of sealwort and barley for 15-20 minutes; then adjust the temperature of the mixture to 80-95°C, adjust the pH to 5.5-6.0 with alkali/acid solution, add 80-120U/g liquefaction enzyme at 80-95°C Liquefaction under the condition of 60-80min; then reduce the temperature of the liquefied mixed solution to 58-65℃, add 90-150U/g glucoamylase and saccharify at 58-65℃ for 80-120min, and finally obtain the saccharification solution of sealwort barley; 步骤三,将活性干酵母在含有2%糖的水溶液37℃条件下活化20-30min;Step 3, activating the active dry yeast in an aqueous solution containing 2% sugar at 37°C for 20-30min; 步骤三,在黄精薏仁糖化液品温降低至26-30℃时接种活性干酵母和米根霉,酿酒酵母接种量为0.2-0.4%,生香酵母接种量为0.3-0.6%,米根霉使用菌丝接种,在30℃的条件下前发酵培养,经过12-14h进行头耙,降低品温,之后的发酵过程中按照发酵进程加入糖液并开耙一次,目的是降低品温和使发酵均匀进行;7-9d后,品温与室温相接近,此时发酵阶段结束;Step 3: Inoculate active dry yeast and Rhizopus oryzae when the product temperature of Polygonatum coix seed saccharification liquid is reduced to 26-30°C, the inoculation amount of Saccharomyces cerevisiae is 0.2-0.4%, the inoculum amount of aromatic yeast is 0.3-0.6%, and the inoculum amount of Rhizopus oryzae is 0.2-0.4%. Use mycelium to inoculate, pre-ferment and cultivate under the condition of 30°C, after 12-14h, carry out head raking to lower the product temperature, and then add sugar solution according to the fermentation process and rake once in the subsequent fermentation process, the purpose is to reduce the product temperature and make the fermentation Carry out evenly; After 7-9 days, the product temperature is close to room temperature, and the fermentation stage is over at this time; 步骤四,将经过发酵的醪液压榨过滤,得到滤液,在低温条件下静置澄清或用硅藻土精滤,90-95℃灭菌后进行勾兑即为成品酒。Step 4: Squeeze and filter the fermented mash to obtain filtrate, set it aside for clarification at low temperature or fine filter with diatomaceous earth, sterilize at 90-95°C and blend to obtain finished wine. 10.根据权利要求1-9任一所述的方法制备得到的黄精薏仁保健酒。10. Polygonatum Polygonatum and Job's tears health wine prepared according to any one of claims 1-9.
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