CN108164135A - A kind of boron phosphorus system bioactivity glass and preparation method thereof - Google Patents
A kind of boron phosphorus system bioactivity glass and preparation method thereof Download PDFInfo
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- CN108164135A CN108164135A CN201810099490.3A CN201810099490A CN108164135A CN 108164135 A CN108164135 A CN 108164135A CN 201810099490 A CN201810099490 A CN 201810099490A CN 108164135 A CN108164135 A CN 108164135A
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- glass
- boron
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- phosphorus system
- boron phosphorus
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- 239000011521 glass Substances 0.000 title claims abstract description 94
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- GDFCWFBWQUEQIJ-UHFFFAOYSA-N [B].[P] Chemical compound [B].[P] GDFCWFBWQUEQIJ-UHFFFAOYSA-N 0.000 title claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 14
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052796 boron Inorganic materials 0.000 claims abstract description 13
- 238000002844 melting Methods 0.000 claims abstract description 12
- 230000008018 melting Effects 0.000 claims abstract description 12
- KKCBUQHMOMHUOY-UHFFFAOYSA-N Na2O Inorganic materials [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 claims abstract description 11
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 11
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims abstract description 11
- IATRAKWUXMZMIY-UHFFFAOYSA-N strontium oxide Inorganic materials [O-2].[Sr+2] IATRAKWUXMZMIY-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 239000010452 phosphate Substances 0.000 claims abstract description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 4
- -1 phosphonium ion Chemical class 0.000 claims abstract description 3
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims abstract 2
- 239000000428 dust Substances 0.000 claims description 24
- 238000007598 dipping method Methods 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 13
- 239000012498 ultrapure water Substances 0.000 claims description 13
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical group [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 8
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 8
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 8
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 8
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 5
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 239000002202 Polyethylene glycol Substances 0.000 claims description 3
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229920001223 polyethylene glycol Polymers 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000003828 vacuum filtration Methods 0.000 claims description 3
- 229920002385 Sodium hyaluronate Polymers 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 229940010747 sodium hyaluronate Drugs 0.000 claims 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims 1
- 230000008520 organization Effects 0.000 abstract description 5
- 239000005385 borate glass Substances 0.000 abstract description 3
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 230000001771 impaired effect Effects 0.000 abstract description 3
- 239000012620 biological material Substances 0.000 abstract description 2
- 238000001727 in vivo Methods 0.000 abstract description 2
- 230000029663 wound healing Effects 0.000 description 16
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 11
- 238000004113 cell culture Methods 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
- 239000013068 control sample Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 238000000498 ball milling Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000011056 performance test Methods 0.000 description 4
- 229910052697 platinum Inorganic materials 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 206010052428 Wound Diseases 0.000 description 3
- 238000002803 maceration Methods 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 2
- 206010039203 Road traffic accident Diseases 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000003749 cleanliness Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 229910052712 strontium Inorganic materials 0.000 description 2
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- DMGNFLJBACZMRM-UHFFFAOYSA-N O[P] Chemical compound O[P] DMGNFLJBACZMRM-UHFFFAOYSA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000005313 bioactive glass Substances 0.000 description 1
- 239000005312 bioglass Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000005365 phosphate glass Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C3/00—Glass compositions
- C03C3/12—Silica-free oxide glass compositions
- C03C3/14—Silica-free oxide glass compositions containing boron
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/10—Ceramics or glasses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C12/00—Powdered glass; Bead compositions
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C4/00—Compositions for glass with special properties
- C03C4/0007—Compositions for glass with special properties for biologically-compatible glass
-
- C—CHEMISTRY; METALLURGY
- C03—GLASS; MINERAL OR SLAG WOOL
- C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
- C03C4/00—Compositions for glass with special properties
- C03C4/0007—Compositions for glass with special properties for biologically-compatible glass
- C03C4/0014—Biodegradable glass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/112—Phosphorus-containing compounds, e.g. phosphates, phosphonates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Geochemistry & Mineralogy (AREA)
- General Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Ceramic Engineering (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Glass Compositions (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a kind of boron phosphorus system bioactivity glass and preparation method thereof, prepare boron system glass by melting cooling method, raw material composition is B2O3、MgO、CaO、Na2O、K2O and SrO, mass ratio are 45 ~ 60：2~10：15~25：3~10：10~15：1~8.Then it is impregnated by the solution containing phosphonium ion, obtains porous phosphate as shell, borate is the double-deck bioactivity glass of kernel.By porous shell, the degradation rate of borate glass structure in vivo can not only be controlled, and the biocompatibility of biomaterial can be improved, so as to be obviously improved the reparation speed of impaired body surface organization.
Description
Technical field
The invention belongs to bio-medical technology fields, and in particular to a kind of bioactivity for body surface organization's injury repair
Glass and preparation method thereof.
Background technology
Today's society science and technology develops rapidly, and vehicles type and quantity are all on the increase, and Ge great Yi, tier 2 cities all go out
The crowded situation of traffic transition is showed, these variations directly result in traffic accident and take place frequently, and lead to personnel death and number of injured people
Increase severely.In major traffic accident, body surface is wound, and patient is in the majority, and acceleration body surface organization injury repair becomes everybody demand.
On the other hand, the time of healing the wounds can be reduced by body surface organization being accelerated to repair when body surface is impaired, create more social values,
Numerous studies have been carried out to bio-vitric in the world.From the silicate systems bio-vitric that professor Hench proposes to quotient in 1971
The 45S5 bioactivity glass of industry, the performance of material are constantly promoted（Zhong Jipin, Journal of Inorganic Materials, 1995）, also emerge in large numbers
A large amount of patent, such as CN201210518111.2 provide and make the preparation of the body of biological glass powder containing strontium by sol-gal process
Method, still further provide make the stent of Porous bioglass containing strontium preparation method, such as CN201710198500.4,
A kind of bio-vitric dressing and its drug delivery device for uterine neck wound healing is provided, but public technology focuses mostly on greatly at present
In phosphate and silicate systems bio-vitric system, shortcoming is that degradation rate of the two in human body is excessively slow, shadow
The growth of tissue is rung, so as to influence wound healing.
Invention content
The purpose of the present invention is by a kind of easy dipping method, prepare the borophosphate bioactivity glass of nucleocapsid
Glass obtains excellent tissue repairing ability and biocompatibility.The present invention is to be implemented by following technical solution：
A kind of boron phosphorus system bioactivity glass and preparation method thereof, selected initial glass are boron system glass, and raw material composition is
B2O3、MgO、CaO、Na2O、K2O and SrO, mass ratio are 45 ~ 60：2~10：15~25：3~10：10~15：1~8.Initial glass
Glass frit is made using the method that high-temperature fusion cools down, glass powder is obtained after ball milling is sieved.Glass melting temperature is 1000-
1350 DEG C, soaking time is 0.5 ~ 4 hour, preferably 1100-1300 DEG C, keeps the temperature 0.5-2 hours.Concrete operation step is as follows：
（1）The experimental apparatus arrived used in this method, such as beaker, stirring rod, graduated cylinder are cleaned, and disappear with ultra-pure water
Malicious sterilization processing；
（2）Boron system glass is ground into sieving, obtains 30 ~ 45 μm of glass dust of grain size, and the sterilization processing that carries out disinfection to glass dust；
（3）Raw material needed for dipping solution is configured according to proportioning, it is spare after stirring and dissolving；The solution composition of dipping is CaCl2、
(NH4)2HPO4、K2HPO4×3H2O, HCl is dissolved in the ultra-pure water of 1L, and mass ratio is 5 ~ 20：15~25：60~70：0.1~
2；The CaCl2、(NH4)2HPO4、K2HPO4×3H2O is to analyze pure, a concentration of 0.1mol/L of HCl；
（4）100 g glass powders are weighed, are put into 1L dipping solutions, persistently stir 1 ~ 8h, preferably 3-6h；
（5）Glass dust after dipping is carried out by separation of solid and liquid using vacuum filtration process；
（6）Glass dust after separation is cleaned with ultra-pure water in vacuum apparatus, it is spare after drying；
（7）Medical solvent is added in into glass dust, bio-vitric is prepared into and applies dressing.
The step（1）In, appliance need to carry out disinfection sterilization, and operating personnel and environment also require to keep the nothing of height
Bacterium state ensures not introduce harmful substance in experimentation；
The step（2）In, the abrasive media that the grinding of bio-vitric uses is agate mortar；The sieve used is food grade
Stainless steel sieves, and sieve is sieved using 325 mesh, 270 mesh, and it is spare that middle layer glass dust is taken after sieving.
The step（3）In, used hydrochloric acid main purpose is to adjust the pH value of solution, is added after the completion of solution is prepared
Enter, the acid-base value for making solution is in faintly acid.
The step（4）In, it needs to confirm the homodisperse of glass dust in whipping process, it is impossible to have caking or aggregate, protect
Card glass dust and maceration extract come into full contact with.
The step（5）In, vacuum filter is less than 30 microns of filter paper using aperture, and control solution instills speed and vacuum
Degree prevents pore plugging and filter paper damaged.
The step（6）In, ultra-pure water cleaning carries out 3 cycles, it is ensured that glass powder cleaning is comprehensive.
The step（7）Used in medical solvent for atoleine, polyethylene glycol, carboxymethyl chitosan, hyalomitome
The mixture of sour sodium etc.；
The step（1）~（7）It need to all be carried out in the higher bio-medical laboratory of cleanliness factor, ensure the accuracy of experimentation.
The remarkable advantage of the present invention is：
（1）Melting cooling method prepares boron system glass, is then impregnated by the solution containing phosphonium ion, obtains porous phosphate glass
For shell, borate glass is the double-deck bioactivity glass of kernel；
（2）Using porous phosphate shell, the degradation rate of borate glass structure in vivo can not only be controlled, and
And the biocompatibility of biomaterial can be improved, it is obviously improved the reparation speed of impaired body surface organization；
（3）By adjusting the calcium ion concentration in maceration extract, suitable hydroxyl phosphorus can also be formed in bioactive glass surface
Lime stone effectively facilitates bio-vitric dressing and the complexing of tissue；
（4）The raw material of the present invention is simple and easy to get, and preparation method is easy, process stabilizing, has reached practical and industrialized condition.
Description of the drawings
Fig. 1 is the wound healing situation of control sample；
Fig. 2 is the wound healing situation after the glass use of boron phosphorus system；
Fig. 3 is the Cell culture invitro comparison diagram of each embodiment.
Specific embodiment
A kind of boron phosphorus system bioactivity glass and preparation method thereof, selected initial bio-vitric be boron system glass, raw material
It forms as B2O3、MgO、CaO、Na2O、K2O and SrO, mass ratio are 45 ~ 60：2~10：15~25：3~10：10~15：1~8.Just
The glass melting temperature of beginning glass is 1000-1350 DEG C, and soaking time is 0.5 ~ 4 hour, preferably 1100-1300 DEG C, keeps the temperature 0.5-2
Hour.The solution composition of dipping is CaCl2、(NH4)2HPO4、K2HPO4×3H2O, HCl is dissolved in the ultra-pure water of 1L, matter
Amount is than being 5 ~ 20：15~25：60~70：0.1~2.Concrete operation step is as follows：
（1）The experimental apparatus arrived used in this method, such as beaker, stirring rod, graduated cylinder are cleaned, and disappear with ultra-pure water
Malicious sterilization processing；
（2）Boron system glass is ground into sieving, obtains 30 ~ 45 μm of glass dust of grain size, and the sterilization processing that carries out disinfection to glass dust；
（3）Raw material needed for dipping solution is configured according to proportioning, it is spare after stirring and dissolving；
（4）100 g glass powders are weighed, are put into 1L dipping solutions, persistently stir 1 ~ 8h, preferably 3-6h；
（5）Glass dust after dipping is carried out by separation of solid and liquid using vacuum filtration process；
（6）Glass dust after separation is cleaned with ultra-pure water in vacuum apparatus, it is spare after drying；
（7）Medical solvent is added in into glass dust, bio-vitric is prepared into and applies dressing.
The step（1）In, appliance need to carry out disinfection sterilization, and operating personnel and environment also require to keep the nothing of height
Bacterium state ensures not introduce harmful substance in experimentation；
The step（2）In, the abrasive media that the grinding of bio-vitric uses is agate mortar；The sieve used is food grade
Stainless steel sieves, and sieve is sieved using 325 mesh, 270 mesh, and it is spare that middle layer glass dust is taken after sieving.
The step（3）In, used hydrochloric acid main purpose is to adjust the pH value of solution, is added after the completion of solution is prepared
Enter, the acid-base value for making solution is in faintly acid.
The step（4）In, it needs to confirm the homodisperse of glass dust in whipping process, it is impossible to have caking or aggregate, protect
Card glass dust and maceration extract come into full contact with.
The step（5）In, vacuum filter is less than 30 microns of filter paper using aperture, and control solution instills speed and vacuum
Degree prevents pore plugging and filter paper damaged.
The step（6）In, ultra-pure water cleaning carries out 3 cycles, it is ensured that glass powder cleaning is comprehensive.
The step（7）Used in medical solvent for atoleine, polyethylene glycol, carboxymethyl chitosan, hyalomitome
The mixture of sour sodium etc.；
The step（1）~（7）It need to all be carried out in the higher bio-medical laboratory of cleanliness factor, ensure the accuracy of experimentation.
Table 1 is the initial glass component table in embodiment 1-4（By mass percentage）
| B2O3 | MgO | CaO | Na2O | K2O | SrO | |
| Control sample | 58 | 2 | 15 | 10 | 15 | 0 |
| Embodiment 1 | 45 | 8 | 20 | 8 | 15 | 4 |
| Embodiment 2 | 50 | 5 | 25 | 5 | 10 | 5 |
| Embodiment 3 | 55 | 2 | 15 | 10 | 15 | 3 |
| Embodiment 4 | 60 | 4 | 15 | 3 | 10 | 8 |
Embodiment 1：The preparation of material and the performance test results
According to the proportioning of each component of table 1, a certain amount of analysis pure raw material is weighed（B2O3、MgO、CaO、Na2O、K2O and SrO）,
Powder is put into platinum crucible after mixing, chamber type electric resistance furnace is placed in and is melted（Glass melting temperature is 1350 DEG C, soaking time 2
Hour）.Melt is then poured into chilling in deionized water, it is dry to obtain glass frit, obtain glass powder after ball milling.Weigh one
Quantitative CaCl2、(NH4)2HPO4、K2HPO4×3H2O、HCl（Its mass ratio is 5：23：70：2）, it is dissolved in the ultra-pure water of 1L
In.100g glass powders are weighed, is put into 1L dipping solutions, persistently stirs 6h.It will be impregnated using vacuum filter after the completion of stirring
Glass dust afterwards is separated, and dries and that bio-vitric dressing is made is spare.Dressing be respectively used to mouse surface Wound healing and
Cell culture experiments in vitro, Wound healing experiment respectively photograph to record the wound healing situation after 1,3,5,7 day, cell in vitro
The sample that culture takes after cultivating 7 days is compared.Fig. 3 is the Cell culture invitro comparison diagram of each embodiment.It can be seen by figure,
The bio-vitric cell survival amount of embodiment 1（8.9*104）Higher than untreated control sample（8.6*104）.
Embodiment 2：The preparation of material and the performance test results
According to the proportioning of each component of table 1, a certain amount of analysis pure raw material is weighed（B2O3、MgO、CaO、Na2O、K2O and SrO）,
Powder is put into platinum crucible after mixing, chamber type electric resistance furnace is placed in and is melted（Glass melting temperature is 1300 DEG C, soaking time 2
Hour）.Melt is then poured into chilling in deionized water, it is dry to obtain glass frit, obtain glass powder after ball milling.Weigh one
Quantitative CaCl2、(NH4)2HPO4、K2HPO4×3H2O、HCl（Its mass ratio is 10：20：68.5：1.5）, it is dissolved in the super of 1L
In pure water.100g glass powders are weighed, is put into 1L dipping solutions, persistently stirs 6h.It will using vacuum filter after the completion of stirring
Glass dust after dipping is separated, and dries and that bio-vitric dressing is made is spare.Dressing is respectively used to mouse surface wound and is cured
It closes and cell culture experiments in vitro, Wound healing experiment respectively photographs to record the wound healing situation after 1,3,5,7 day, in vitro
The sample that cell culture takes after cultivating 7 days is compared.Fig. 3 is the Cell culture invitro comparison diagram of each embodiment.It can by figure
See, the bio-vitric cell survival amount of embodiment 2（9.1*104）It is apparently higher than untreated control sample.
Embodiment 3：The preparation of material and the performance test results
According to the proportioning of each component of table 1, a certain amount of analysis pure raw material is weighed（B2O3、MgO、CaO、Na2O、K2O and SrO）,
Powder is put into platinum crucible after mixing, chamber type electric resistance furnace is placed in and is melted（Glass melting temperature is 1200 DEG C, soaking time 2
Hour）.Melt is then poured into chilling in deionized water, it is dry to obtain glass frit, obtain glass powder after ball milling.Weigh one
Quantitative CaCl2、(NH4)2HPO4、K2HPO4×3H2O、HCl（Its mass ratio is 15：15：69：1）, it is dissolved in the ultra-pure water of 1L
In.100g glass powders are weighed, is put into 1L dipping solutions, persistently stirs 6h.It will be impregnated using vacuum filter after the completion of stirring
Glass dust afterwards is separated, and dries and that bio-vitric dressing is made is spare.Dressing be respectively used to mouse surface Wound healing and
Cell culture experiments in vitro, Wound healing experiment respectively photograph to record the wound healing situation after 1,3,5,7 day, cell in vitro
The sample that culture takes after cultivating 7 days is compared.Fig. 3 is the Cell culture invitro comparison diagram of each embodiment.It can be seen by figure,
The bio-vitric cell survival amount of embodiment 3 is up to 9.6*104, it is most preferred embodiment.In addition, Fig. 1 ~ 2 are control sample glass
With the wound healing situation map of embodiment 3, as seen from the figure, embodiment 3 can effectively facilitate wound healing.
Embodiment 4：The preparation of material and the performance test results
According to the proportioning of each component of table 1, a certain amount of analysis pure raw material is weighed（B2O3、MgO、CaO、Na2O、K2O and SrO）,
Powder is put into platinum crucible after mixing, chamber type electric resistance furnace is placed in and is melted（Glass melting temperature is 1100 DEG C, soaking time 2
Hour）.Melt is then poured into chilling in deionized water, it is dry to obtain glass frit, obtain glass powder after ball milling.Weigh one
Quantitative CaCl2、(NH4)2HPO4、K2HPO4×3H2O、HCl（Its mass ratio is 20：19.9：60：0.1）, it is dissolved in the super of 1L
In pure water.100g glass powders are weighed, is put into 1L dipping solutions, persistently stirs 6h.It will using vacuum filter after the completion of stirring
Glass dust after dipping is separated, and dries and that bio-vitric dressing is made is spare.Dressing is respectively used to mouse surface wound and is cured
It closes and cell culture experiments in vitro, Wound healing experiment respectively photographs to record the wound healing situation after 1,3,5,7 day, in vitro
The sample that cell culture takes after cultivating 7 days is compared.Fig. 3 is the Cell culture invitro comparison diagram of each embodiment.It can by figure
See, the bio-vitric cell survival amount of embodiment 4（9.3*104）It is apparently higher than control sample.
The present invention realizes a kind of boron phosphorus system bioactivity glass and preparation method thereof by above-described embodiment.It is significantly imitated
Fruit embodies a concentrated reflection of excellent biocompatibility and promotes the ability of tissue repair.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification should all belong to the covering scope of the present invention.
Claims (10)
1. a kind of preparation method of boron phosphorus system bioactivity glass, it is characterised in that：Boron system glass is prepared by melting cooling method,
Its raw material composition is B2O3、MgO、CaO、Na2O、K2Then O and SrO is impregnated by the dipping solution containing phosphonium ion, obtain
Porous phosphate is shell, and borate is the double-deck bioactivity glass of kernel.
2. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 1, it is characterised in that：The boron system
Glass, raw material composition are B2O3、MgO、CaO、Na2O、K2O and SrO, mass ratio are 45 ~ 60：2~10：15~25：3~10：10
~15：1~8.
3. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 2, it is characterised in that：The boron system
Glass, raw material composition are B2O3、MgO、CaO、Na2O、K2O and SrO, mass ratio are 50 ~ 58：3~8：15~20：3~10：10~
15：2~7.
4. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 1, it is characterised in that：
The dipping solution ingredient is CaCl2、(NH4)2HPO4、K2HPO4×3H2O, HCl, mass ratio are 5 ~ 20：15~25：60~
70：0.1 ~ 2, it is dissolved in the ultra-pure water of 1L.
5. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 1, it is characterised in that：
Concrete operation step is as follows：
（1）Required experimental apparatus, such as beaker, stirring rod, graduated cylinder with ultra-pure water are cleaned, and carry out disinfection processing；
（2）Boron system initial glass frit is made using the method for melting cooling, boron system glass is ground into sieving, acquisition grain size 30 ~
45 μm of glass dust, and the sterilization processing that carries out disinfection to glass dust；
（3）Raw material needed for dipping solution is configured according to proportioning, it is spare after stirring and dissolving；
（4）100 g glass powders are weighed, are put into 1L dipping solutions, persistently stir 1 ~ 8h；
（5）Glass dust after dipping is carried out by separation of solid and liquid using vacuum filtration process；
（6）Glass dust after separation is cleaned with ultra-pure water in vacuum apparatus, it is spare after drying；
（7）Medical solvent is added in boron glass frit after treatment, bio-vitric is prepared into and applies dressing.
6. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 5, it is characterised in that：
Step（2）The glass melting temperature of boron system initial glass is 1000-1350 DEG C, and soaking time is 0.5 ~ 4 hour.
7. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 6, it is characterised in that：
Glass melting temperature is 1100-1300 DEG C, keeps the temperature 0.5-2 hours.
8. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 5, it is characterised in that：
Step（4）The mixing time that continues is 3-6h.
9. a kind of preparation method of boron phosphorus system bioactivity glass according to claim 5, it is characterised in that：
The step（7）Used in medical solvent for atoleine, polyethylene glycol, carboxymethyl chitosan, Sodium Hyaluronate
Mixture.
10. a kind of boron phosphorus system bioactivity glass prepared by method as described in claim 1.
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| PCT/CN2018/077009 WO2019148554A1 (en) | 2018-02-01 | 2018-02-23 | Boron and phosphorus-based bioactive glass and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109437559A (en) * | 2018-11-19 | 2019-03-08 | 湖北中创医疗用品有限公司 | Bioactivity glass, bioactivity glass gel and its preparation method and application |
| CN109985272A (en) * | 2019-03-07 | 2019-07-09 | 中国科学院合肥物质科学研究院 | Borophosphate bioglass composite material and preparation method and use thereof |
| WO2020047662A1 (en) * | 2018-09-05 | 2020-03-12 | Ir Scientific Inc. | Glass composition |
| WO2021179071A1 (en) * | 2020-03-09 | 2021-09-16 | Ir Scientific Inc. | Glass composition |
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|---|---|---|---|---|
| US6709744B1 (en) * | 2001-08-13 | 2004-03-23 | The Curators Of The University Of Missouri | Bioactive materials |
| WO2014159240A1 (en) * | 2013-03-14 | 2014-10-02 | Novabone Products, Llc | Compositions and methods for manufacturing sol-gel derived bioactive borophosphate glasses for medical applicatons |
| CN106630646A (en) * | 2016-12-09 | 2017-05-10 | 苏州纳贝通环境科技有限公司 | Porous biological glass ceramic and preparation method thereof |
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| CN102430149A (en) * | 2011-11-18 | 2012-05-02 | 华东理工大学 | Boron phosphate glass fiber/chitosan composite material |
| CN107617121B (en) * | 2017-10-13 | 2020-09-01 | 广州润虹医药科技股份有限公司 | Biological induction active dressing for skin wound surface and preparation method and application thereof |
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- 2018-02-23 WO PCT/CN2018/077009 patent/WO2019148554A1/en active Application Filing
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6709744B1 (en) * | 2001-08-13 | 2004-03-23 | The Curators Of The University Of Missouri | Bioactive materials |
| WO2014159240A1 (en) * | 2013-03-14 | 2014-10-02 | Novabone Products, Llc | Compositions and methods for manufacturing sol-gel derived bioactive borophosphate glasses for medical applicatons |
| CN106630646A (en) * | 2016-12-09 | 2017-05-10 | 苏州纳贝通环境科技有限公司 | Porous biological glass ceramic and preparation method thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020047662A1 (en) * | 2018-09-05 | 2020-03-12 | Ir Scientific Inc. | Glass composition |
| EP3847139A4 (en) * | 2018-09-05 | 2022-06-15 | IR Scientific Inc. | Glass composition |
| US12076424B2 (en) | 2018-09-05 | 2024-09-03 | Ir Scientific Inc. | Glass composition |
| CN109437559A (en) * | 2018-11-19 | 2019-03-08 | 湖北中创医疗用品有限公司 | Bioactivity glass, bioactivity glass gel and its preparation method and application |
| CN109437559B (en) * | 2018-11-19 | 2021-09-14 | 湖北中创医疗用品有限公司 | Bioactive glass, bioactive glass gel, and preparation method and application thereof |
| CN109985272A (en) * | 2019-03-07 | 2019-07-09 | 中国科学院合肥物质科学研究院 | Borophosphate bioglass composite material and preparation method and use thereof |
| CN109985272B (en) * | 2019-03-07 | 2022-05-13 | 中国科学院合肥物质科学研究院 | Borophosphate bioglass composite material and preparation method and use thereof |
| WO2021179071A1 (en) * | 2020-03-09 | 2021-09-16 | Ir Scientific Inc. | Glass composition |
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| CN108164135B (en) | 2019-07-05 |
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