CN108159439A - A kind of aseptic operating inoculator Quick sterilizing device and its application method - Google Patents

Abstract

The invention discloses a rapid sterilization device for aseptic operation inoculation apparatus, which comprises an electric control system arranged in an upper casing and an electromagnetic induction system arranged in a lower casing, the upper casing and the lower casing are connected and fixed through a connecting column; the electromagnetic induction The system includes a ceramic cylinder for placing the utensils to be sterilized inside, an induction coil wound around the ceramic cylinder, and a temperature sensor set inside the ceramic cylinder; the power control system includes a power supply, a controller, a high-voltage driver board, a step-down module and a relay The temperature sensor is electrically connected to the controller, the negative pole of the high-voltage driver board is connected to the negative pole of the power supply, the positive pole of the high-voltage driver board is connected to the relay group, the relay group is connected to the positive pole of the step-down module, and the relay group is electrically connected to the controller. The rapid sterilizing device for aseptic operation inoculation equipment and the use method thereof of the present invention can improve work efficiency, expand the types of inoculation equipment to be sterilized, protect some equipment from damage, reduce energy consumption and ensure safe production.

Description

A rapid sterilization device for aseptic operation inoculation equipment and its use method

technical field

The invention relates to the technical field of sterilizing devices, in particular to a rapid sterilizing device for aseptically operated inoculation instruments and a method for using the same.

Background technique

In the fields of plant tissue culture, microbial engineering, medicine, cell engineering and molecular biology, etc., the sterilization of inoculation equipment in the process of aseptic operation is involved, and the sterilization effect, sterilization speed, work efficiency and operation process of the equipment Safety and other aspects have always been the research and development hotspots in this field.

Twenty years ago, alcohol lamp flame heating was used to sterilize and sterilize. This method has a thorough disinfection effect and low cost, but the biggest defect is that there is a great potential safety hazard. Fire problems caused by alcohol lamps often occur in laboratories. In the past ten years, we have gradually seen sterilization devices that replace alcohol lamps. For example, the patent number is CN200620080704.5, and the patent name is "Quick disinfection device for aseptic operation inoculation equipment". The principle is based on radiation-focused heating and has the characteristics of rapid temperature rise. , but the self-generating heat of the equipment is high, the energy consumption is too high, and the line is under great pressure, so there are still certain safety hazards; the patent number is CN200920115225.6, and the patent name is "a sterilizer for tissue culture inoculation equipment". Heating conducts heat through ceramic containers and quartz sand to realize high-temperature sterilization of inoculation instruments; the heating principle of the JZ-2 digital display (JZ-1 knob type) inoculation instrument sterilizer commonly used in tissue culture production today is also resistance wire heating The high-temperature sterilization of the inoculation equipment is realized through the heat conduction of the ceramic container and the quartz sand. Quartz sand has the effect of heat preservation, but due to the tight arrangement of quartz sand in the ceramic container, huge resistance and friction will be generated during the insertion of the appliance, especially for sharp and thin scalpel blades, which will cause serious damage, and the device starts to heat up slowly , It takes about 20 minutes to reach the preset temperature above 260°C after the power switch of the equipment is turned on, and the working efficiency is low. Secondly, the temperature of the ceramic container itself reaches 260°C, which will bring a lot of radiant heat to the equipment shell, which is easy to burn the operator and cause high temperature in the working environment. In short, all of the above sterilization methods: alcohol lamp flame method, resistance wire heating, fire roasting, infrared lamp roasting, etc. all have the ability to High energy consumption, low thermal efficiency, insufficient safety and other defects.

Contents of the invention

The purpose of the present invention is to provide a rapid sterilization device for aseptic operation inoculation equipment and its use method, to solve the problems in the above-mentioned prior art, to improve work efficiency, expand the types of inoculation equipment to be sterilized, and make some equipment avoid damage, reduce energy consumption and produce safely.

In order to achieve the above object, the present invention provides the following solution: the present invention provides a rapid sterilization device for aseptic operation inoculation equipment, which includes an electric power control system arranged in the upper casing and an electromagnetic induction system arranged in the lower casing. The shell and the lower shell are connected and fixed through connecting columns; the electromagnetic induction system includes a ceramic cylinder for placing the utensils to be sterilized inside, an induction coil wound around the periphery of the ceramic cylinder, and a temperature setting inside the ceramic cylinder. sensor; the power control system includes a power supply, a controller, a high-voltage drive board, a step-down module and a relay group, the temperature sensor is electrically connected to the controller, the negative pole of the high-voltage drive board is connected to the negative pole of the power supply, The positive pole of the high-voltage driving board is connected to the relay group, the relay group is connected to the positive pole of the step-down module, and the relay group is electrically connected to the controller.

Optionally, the cylinder body of the ceramic cylinder is placed inside the lower casing, and the mouth of the ceramic cylinder is 2 cm exposed from the lower casing.

Optionally, the induction coil is evenly wound around the periphery of the cylindrical ceramic cylinder and fixed by high temperature resistant silicone sealant; the induction coil is an oxygen-free copper induction coil.

Optionally, the temperature sensor is arranged at the central axis of the ceramic cylinder, and the temperature sensor is connected to the digital display temperature control unit in the controller.

Optionally, a temperature display adjustment screen and a power switch are arranged on the outside of the upper casing, the temperature display adjustment screen is connected to the digital display temperature control unit, and the power switch is connected to the controller; The display temperature control unit is used to control the work of the relay group.

Optionally, an iron casing is provided on the outside of the temperature sensor.

Optionally, a power line and an induction coil are connected to the high-voltage driving board, and the power line is connected to the power supply.

Optionally, a fan is arranged on the top of the upper casing, and a protective net is arranged on the outside of the fan; one side of the upper casing is also provided with evenly distributed cooling holes; the bottom of the lower casing is symmetrically installed There is a device base; the power supply is an external DC power supply.

The present invention also provides a method for using a rapid sterilization device for aseptic operation inoculation apparatus, which is applied to the above-mentioned rapid sterilization device for aseptic operation inoculation apparatus, comprising the following steps:

1) Connect the power supply: Put the inoculation device to be sterilized into the ceramic cylinder through the entrance of the ceramic cylinder, connect the power interface of the sterilization device with an external DC power supply, and turn on the power switch;

2) The digital display temperature control unit is controlled, the high-voltage drive board works and the relay group works together: the negative pole of the high-voltage drive board is directly connected to the negative pole of the power supply, the positive pole is connected to the common point of the delay relay, and the normally closed point of the delay relay is connected to the equipment. The 15V positive pole is connected, and the normally open point is connected to the positive pole of the step-down module. After the device is powered on, the step-down module enters the working state, and the step-down module steps down the 15V DC to 12V to supply power to the digital display temperature control unit. The digital display temperature control unit enters the working state and receives the feedback signal from the temperature sensor. The control output of the temperature control board of the digital display temperature control unit is 12V, which controls the pull-in state of one relay. The normally open point of one relay is empty, and the common point is 12V positive pole, the normally closed point is connected to the positive pole of the delay relay power supply, and the negative pole of the delay relay is connected to the 12V negative pole;

When the feedback signal is lower than the preset temperature, the temperature control board controls one relay to pull in. At this time, the delay relay does not work, so the high-voltage drive board works under the voltage of 15V; when the temperature rises to the predetermined value, one relay is disconnected, normally The closed point starts to supply power to the delay relay. At this time, the high-voltage drive board will continue to work at 15V for 30 seconds, and the temperature will rise by 20 degrees. After 30 seconds, the delay relay will be closed, and the high-voltage drive board will be switched to 12V power supply. It will drop slowly. When the temperature control board detects that the temperature has dropped to the set value, it will control a relay to pull in. At this time, the delay relay is disconnected, and the high-voltage drive board is switched to 15V power supply again to heat up, and so on until the completion of the cycle. Sterilization of inoculation equipment;

3) Sterilization is completed: Sterilize in the above step 2) to meet the sterility requirements of the inoculation device, turn off the power, take out the inoculation device, and use it after cooling to a suitable temperature.

Compared with the prior art, the present invention has achieved the following technical effects:

1. High efficiency and energy saving. In the present invention, it only takes 3-5 minutes from cold startup to temperature rising to 260-280°C. Through electromagnetic induction, the metal inoculation equipment inside the ceramic cylinder can be fully and uniformly heated and sterilized at high temperature. The average preheating time is 4/5 shorter than that of the resistance coil heating method. At the same time, the thermal efficiency is as high as 80%, and the power saving effect can reach 30. %-60%, the peak power consumption of the entire device is 90 watts.

2. Reduce maintenance cost. The invention separates the electric power, the control system and the induction heating system up and down, which can properly shield the electromagnetic interference on the circuit, and can avoid the failure of components due to high temperature aging; the induction coil itself will not generate excessive heat during normal operation, and the temperature of the core circuit board The temperature is about 30°C, and the temperature of the coil and ceramic cylinder is about 90°C, which can effectively prevent the premature aging of the main body of the equipment, and the service life can reach more than 5 years; the equipment is separated from the power supply, and the overall design of the equipment is scientific and reasonable, which can significantly reduce maintenance costs.

3. Safe operation. The high-voltage drive board of the present invention adopts the most advanced industrial PCB circuit board at present, the power supply of the equipment is 15V, the maximum current is 6A, its own calorific value is very small and it has a perfect heat dissipation function, which can fully guarantee the long-term safe and stable operation of the equipment; There is no heat-conducting quartz in the cylinder, and the contact surface between the appliance and the container is small, so it is difficult for the temperature of the appliance to be effectively transmitted to the ceramic cylinder in large quantities. The temperature of the ceramic cylinder mainly comes from the self-heating of the induction coil. After working stably for a long time, the temperature of the ceramic cylinder is only 90°C, and the heat radiated to the equipment shell is relatively low. , can achieve safe production.

4. Easy and efficient operation. The present invention has removed the resistance of the quartz beads in the past disinfection core, no matter various types (such as tweezers placing brackets, scissors, etc.) and models of inoculation or related utensils can be put into ceramic cylinders for heating and sterilization; the induction cylinder volume is large, The caliber is large, and multiple sets of inoculation instruments can be put in at the same time and used alternately without special sterilization time. The operation is simple and practical, and the work efficiency is improved.

5. The working environment is comfortable. Because the equipment itself does not emit high heat, the present invention greatly improves the temperature of the working environment at the production site, improves the comfort of the human body, creates a more green, energy-saving, safe and comfortable production environment, and reduces cooling costs .

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the embodiments. Obviously, the accompanying drawings in the following description are only some of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without paying creative labor.

Fig. 1 is the internal structure schematic diagram of aseptic operation inoculation device rapid sterilization device among the present invention;

Fig. 2 is the appearance schematic diagram of the quick sterilization device of aseptic operation inoculation apparatus in the present invention;

Among them, 1 temperature display adjustment screen; 2 upper shell; 3 power switch; 4 controller; 5 relay group; 6 high voltage drive board; 7 cooling fan; 8 power interface; ; 12 ceramic cylinder; 13 induction coil; 14 equipment base; 15 entrance of ceramic cylinder.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

The purpose of the present invention is to provide a rapid sterilization device for aseptic operation inoculation utensils and its use method, to solve the problems in the above-mentioned prior art, to improve work efficiency, expand the types of inoculation utensils to be sterilized, and prevent some utensils from damage, reduce energy consumption and produce safely.

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

Please refer to Figures 1-2, wherein, Figure 1 is a schematic diagram of the internal structure of the rapid sterilization device for aseptic operation inoculation equipment in the present invention; Figure 2 is a schematic diagram of the appearance of the rapid sterilization device for aseptic operation inoculation equipment in the present invention.

As shown in Figures 1-2, the present invention provides a rapid sterilization device for aseptic operation inoculation instruments, including a power control system arranged in the upper casing 2 and an electromagnetic induction system arranged in the lower casing 10, the upper casing 2. It is connected and fixed with the lower casing 10 through the connecting column 9; the electromagnetic induction system includes a ceramic cylinder 12 for placing the utensils to be sterilized inside, an induction coil 13 wound around the periphery of the ceramic cylinder 12, and a temperature sensor arranged inside the ceramic cylinder 12 11. The power control system includes a power supply, a controller 4, a high-voltage drive board 6, a step-down module and a relay group 5, the temperature sensor 11 is electrically connected to the controller 4, the negative pole of the high-voltage drive board 6 is connected to the negative pole of the power supply, and the high-voltage drive board 6 The positive pole of the relay is connected to the relay group 5, the relay group 5 is connected to the positive pole of the step-down module, and the relay group 5 is electrically connected to the controller 4.

The cylinder body of the ceramic cylinder 12 is placed inside the lower housing 10, and the ceramic cylinder inlet 15 exposes about 2cm outside the lower housing 10; Extend the temperature sensor 11 into the interior of the ceramic cylinder 12; the temperature sensor 11 is connected to the digital display temperature control unit in the controller 4; the induction coil 13 is evenly wound around the periphery of the cylindrical ceramic cylinder 12 and covered with a high-temperature resistant silicone sealant Fixed, the induction coil 13 is a 4 square national standard oxygen-free copper hard wire, and it only takes 3 to 5 minutes to heat up to 260°C after being energized and placed in the inoculation device, which is more energy-saving and time-saving, and greatly reduces power consumption. The outer side of the temperature sensor 11 is provided with an iron shell, and the temperature sensor 11 wrapped inside can detect the change of the sensor shell temperature. After being put into the cold appliance, the magnetic field lines of the temperature sensor 11 shell are weakened by the change and the temperature drops, and then heats up together with the appliance.

There are four base reserved holes on the top of the shell of the controller 4, which are covered with plastic nails, and the positions of the sensing system and the control system can be exchanged according to the working environment and usage requirements.

The outer side of the upper shell 2 is provided with a temperature display adjustment screen 1 and a power switch 3, the temperature display adjustment screen 1 is connected to the digital display temperature control unit, and the power switch 3 is connected to the controller 4; the digital display temperature control unit is used to control the relay group 5 Work. The relay group 5 is responsible for switching between 12V and 15V, and selects a suitable voltage to supply the high-voltage drive board 6 .

The high-voltage driving board 6 is connected with a power line and an induction coil 13, and the power line is connected with the power supply.

The upper and lower housings 10 are made of stainless steel (or aluminum alloy), which is beneficial to heat dissipation, anti-corrosion, anti-rust and beautiful; the top of the upper housing 2 is provided with a cooling fan 7, and the outer side of the cooling fan 7 is provided with a protective net, which is made of stainless steel The grid-like structure is used to prevent the operator from contacting the fan or preventing debris from being involved in the fan; one side of the upper casing 2 is also provided with evenly distributed cooling holes; the bottom of the lower casing 10 is symmetrically installed with a device base 14; The power supply is an external DC power supply.

The digital display temperature control unit includes temperature control and digital display. The temperature controller is powered by stepping down the 15V voltage of the equipment to 12V DC through the LM2596SDC-DC step-down module. The temperature measuring head adopts Omega K-type GG-K-36 resistant High temperature temperature sensor 11. In order to be safer, the lead-in wires inside the controller are made of high-temperature-resistant aviation wires, and then covered with high-temperature-resistant casings to ensure temperature and pressure resistance and a longer service life.

The operation of the whole device is controlled by the temperature control board, the high-voltage drive board 6 works and the relay group 5 cooperates to complete. The negative pole of the high-voltage drive board 6 is directly connected to the negative pole of the power supply, and the positive pole is connected to the common point of the time-delay relay. The open point is connected to the positive pole of the XL4015 high-current step-down module. The purpose of the XL4015 high-current step-down module is to provide a stable voltage and current of 12V5A for the high-voltage driver board 6. After the device is powered on, the LM2596S step-down module and the XL4015 high-current step-down module start to work state, the LM2596S step-down module steps down the 15V DC to 12V to supply power to the temperature control board. At this time, the temperature control board enters the working state and receives the feedback signal from the temperature sensor 11. In the pull-in state, the normally open point of one relay is empty, the common point is the 12V positive pole, the normally closed point is connected to the positive pole of the delay relay power supply, and the negative pole of the delay relay is connected to the 12V negative pole. When the feedback signal is lower than the preset temperature, the temperature control board controls one relay to pull in. At this time, the delay relay does not work, so the high-voltage drive board 6 works at a voltage of 15V. When the temperature rises to a predetermined value, one relay is disconnected. The normally closed point starts to supply power to the delay relay. At this time, the high-voltage drive board 6 will continue to work at 15V for 30 seconds, and the temperature can rise by about 20 degrees. After 30 seconds, the delay relay is closed, and the high-voltage drive board 6 is switched to 12V Power supply. In this mode, the temperature will drop slowly. When the temperature control board detects that the temperature has dropped to the set value, it will control a relay to pull in. At this time, the delay relay is disconnected, and the high-voltage drive board 6 is switched to 15V power supply again to increase the temperature. , and so on.

Before use, plug the power interface 8 into an external power supply, turn on the device power switch 3, and the inoculation device in the ceramic cylinder 12 can heat up quickly after the power is turned on, and the heating temperature is controlled at 260°C through the digital display temperature control unit (inside the controller 4). Between ±20°C, the temperature will automatically complete the high-temperature sterilization of the inoculation equipment within 15 to 60 seconds, which can instantly kill Staphylococcus aureus, mold and other bacteria and polluting microorganisms, and meet the sterility requirements of the inoculation equipment. At this point, you can take out the inoculation utensil and wait for it to cool down to a suitable temperature before using it.

It should be noted that the selection of the relevant power supply voltage and other numerical values of the rapid sterilization device for aseptic operation inoculation equipment in the present invention is not limited to the numerical values in the examples, and can be adaptively changed according to specific experimental operations , are also within the protection scope of the present invention.

In the present invention, specific examples have been used to illustrate the principle and implementation of the present invention. The description of the above embodiments is only used to help understand the method and core idea of the present invention; meanwhile, for those of ordinary skill in the art, according to the present invention The idea of the invention will have changes in the specific implementation and scope of application. In summary, the contents of this specification should not be construed as limiting the present invention.

Claims (9)

1. a kind of aseptic operating inoculator Quick sterilizing device, it is characterised in that：Including the electric power control being arranged in upper shell System processed and the EM induction system being arranged in lower housing, the upper shell connect fixation with lower housing by connecting pole；Institute It states EM induction system and includes inside for placing the ceramic cylinder of utensil subject to sterilization, being wrapped in the line of induction of the ceramic cylinder periphery Enclose and be arranged on the temperature sensor inside the ceramic cylinder；The electric control system includes power supply, controller, high pressure and drives Movable plate, voltage reduction module and relay group, the temperature sensor are electrically connected with the controller, the cathode of the high drive plate It is connected with the power cathode, the anode of the high drive plate is connected in the relay group, the relay group and institute The anode for stating voltage reduction module is connected, and the relay group is electrically connected with the controller.

2. aseptic operating inoculator Quick sterilizing device according to claim 1, it is characterised in that：The ceramic cylinder Cylinder body is placed in inside the lower housing, and the oral area of the ceramic cylinder exposes the lower housing 2cm.

3. aseptic operating inoculator Quick sterilizing device according to claim 1, it is characterised in that：The induction coil Uniform winding is fixed in the ceramic cylinder periphery of cylindrical type and by thermostable silicone sealant；The induction coil is oxygen-free copper Induction coil.

4. aseptic operating inoculator Quick sterilizing device according to claim 1, it is characterised in that：The temperature sensing Device is arranged on the location mid-shaft of the ceramic cylinder, and the temperature sensor is connected with the digital display temperature-control unit in the controller It connects.

5. aseptic operating inoculator Quick sterilizing device according to claim 4, it is characterised in that：Outside the upper shell Side is provided with temperature display and adjusts screen and power switch, and the temperature display adjusts screen and connect with the digital display temperature-control unit, institute Power switch is stated to connect with the controller；The digital display temperature-control unit is used to that the relay group to be controlled to work.

6. aseptic operating inoculator Quick sterilizing device according to claim 1, it is characterised in that：The temperature sensing Iron shell is provided on the outside of device.

7. aseptic operating inoculator Quick sterilizing device according to claim 1, it is characterised in that：The high drive Power cord and induction coil are connected on plate, the power cord is connect with the power supply.

8. aseptic operating inoculator Quick sterilizing device according to claim 1, it is characterised in that：The upper shell top Portion is provided with fan, and protection network is equipped on the outside of the fan；The side of the upper shell is additionally provided with equally distributed heat emission hole； The bottom end of the lower housing is symmetrically installed with plant bottom case；The power supply is external DC power supply.

9. a kind of application method of aseptic operating inoculator Quick sterilizing device, applied to any one of claim 1-8 institutes The aseptic operating inoculator Quick sterilizing device stated, it is characterised in that：

Include the following steps：

1) power supply is connected：Inoculation utensil subject to sterilization is put by ceramic cylinder entrance in ceramic cylinder, by the electricity of bactericidal unit Source interface is connect with external DC power supply, is turned on the power switch；

2) control of digital display temperature-control unit, the acting of high drive plate and relay group cooperate：The cathode of high drive plate is direct It is connected with power cathode, anode is connected on the common point of time-delay relay, normally closed point and the equipment 15V anodes of time-delay relay Connection, opened point are connected on voltage reduction module anode, and voltage reduction module enters working condition after equipment is powered, by voltage reduction module handle 15V direct currents are depressurized to 12V and power to the digital display temperature-control unit, and the digital display temperature-control unit enters working condition and connects at this time The feedback signal of shrinkage temperature sensor, the temperature control plate control output of digital display temperature-control unit is 12V, controls the actuation of relay all the way State, the opened point of relay is sky all the way, and common point is 12V anodes, and normally closed point connect with time-delay relay power supply anode, prolongs When relay cathode be connected on 12V cathode；

When feedback signal is less than preset temperature, relay is attracted all the way for temperature control plate control, and time-delay relay does not work at this time, institute It is under 15V voltages and done work with high drive plate；After predetermined value is warming up to, all the way relay disconnect, normally closed point start to Time-delay relay power, high drive plate will continue to do work 30 seconds under 15V voltages at this time, heating 20 degree, 30 seconds after be delayed after Electric appliance is attracted, and high drive plate is switched to 12V power supplies, and temperature can slowly decline under this pattern, when temperature control plate detects temperature Relay actuation all the way will be controlled when being down to setting value, time-delay relay disconnects at this time, is switched to again for high drive plate 15V power supply heatings, cycle is until complete the sterilizing to being inoculated with utensil according to this；

3) sterilizing is completed：In above-mentioned steps 2) in sterilize, meet inoculation utensil sterility requirements after, close power supply, take out Utensil is inoculated with, it is to be cooled to can be used to during suitable temperature.