CN108157372A - Bactericidal composition containing dithianon - Google Patents

Abstract

The present invention provides a kind of Synergistic biocidal compositions containing dithianon, it is characterized by comprising active components As and B, wherein, A is dithianon, B is one or more kinds of combinations in Fenamidone, quinoxyfen, the third oxygen quinoline, copper 8-hydroxyquinolinate, sad mantoquita, the auxiliary agent that can be used in Pesticide formulation is further included, conventional pesticidal preparations are made, and applied to controlling plant diseases.

Description

Bactericidal composition containing dithianon

Technical field

The invention belongs to pesticides application technical fields, are related to a kind of composition pesticide, specifically a kind of anthracene containing dicyan The bactericidal composition of quinone, applied to controlling plant diseases.

Background technology

In agricultural production process, plant disease occurs seriously, to endanger the nutrition organs such as axis, leaf and fruit, kind The organs of multiplication such as son, stem tuber position, influences photosynthesis of plant, influences Leaf-feeding insects appearance, crop yield is made to fall sharply, under quality Drop is serious.When disease occurs serious, crop is caused to be had no harvest.Therefore, plant disease in produce reality there is important prevention to anticipate Justice, fungicide are the methods the most economic, quick and effective of controlling plant diseases.

Single bactericide can not be well solved in produce reality using easily developing immunity to drugs or prevention spectrum is limited Plant disease endangers.Mixture fungicide delays the advantages such as phytopathogen drug resistance with that can expand prevention spectrum, enhancing preventive effect, Become most popular application form in production practices.Fungicide especially with synergy, each effective group of fungicide It interacts between point, makes combination aging fruit better than the theoretical summation of exclusive use effect, thus thing can be played in actual use The effect of half work(times, is more favored by user.

Dithianon (dithianon) is the fungicide developed by overseas enterprise, belongs to thiol reaction agent, is mainly used for preventing Scab, anthracnose, ring spot, downy mildew, rust, leaf spot, bacillary spot in the various crops such as strawberry, vegetables, fruit tree The multiple diseases such as point disease.CAS accession number is 3347-22-6.Its structural formula is as follows：

Fenamidone (fenamidone), quinoxyfen (quinoxyfen), the third oxygen quinoline (proquinazid), quinoline Copper (oxine copper), sad mantoquita (copper octanoate) are the fungicide of several broad spectrum activities, in various crop Plant disease show excellent preventive effect.

The present invention is by a large amount of formulation screening test the study found that when dithianon and Fenamidone, quinoxyfen, third When one or more kinds of medicaments in oxygen quinoline, copper 8-hydroxyquinolinate, sad mantoquita arbitrarily combine, surprising sterilization effect is shown Fruit, that is, show good synergistic effect, and practical bactericidal effect is substantially better than the theoretical summation of exclusive use, has in production Very extensive application prospect.

Invention content

The present invention relates to Synergistic biocidal compositions, and including active components A and B, the active components A is dithianon (dithianon), the active constituent B is one in Fenamidone, quinoxyfen, the third oxygen quinoline, copper 8-hydroxyquinolinate, sad mantoquita Kind or more than one combinations.In addition, further include the auxiliary agent that can be used in Pesticide formulation.

The composition can be used to prevent the plant disease in various useful crops with particularly good effect, described useful Crop includes but not limited to conventional breeding crop, genetically modified crops, asexually propagated crop and asexual propagation material, domesticating and cultivating Wild plant etc., especially suitable for prevention vegetables, fruit tree, cotton, potato, peanut, soybean, rape, corn, wheat, tobacco, Multiple diseases on the crops such as ornamental plant.

The representative plant disease of composition for preventing and controlling of the invention, the example can be used for some for the specific example that can be mentioned that It is not limited to specified genus kind.It should be noted that the invention is not limited in examples in detail below, can be extended with same way To other plant diseases, bracket indicates its cause of disease Latin name：

Citrus scab (Sphaceloma fawcettii), yellow twig (Liberobacter asiaticum), mould Sick (Penicillium italicum), peach brown rot (Monilinia fructicola), sweet cherry brown rot (Monilinia laxa), mango blossom-end rot (Phomopsis mangiferae), pear scab (Venturia Nashicola), scab of apple (Venturia inaequalis), powdery mildew (Podosphaera leucotricha), spot It is point defoliation (Alternaria mali), rot disease (Valsa mali), ring spot (Physalospora piricola), brown Pinta (Marssonina coronaria), Cabbage Leaf Spot (Alternaria brassicae), Leaf Spot Caused by Corynespora cassiicola on Cucumber (Corynespora cassiicola), downy mildew (Pseudoperonospora cubensis), downy mildew of garpe (Plasmopara viticola), Chinese cabbage downy mildew (Peronospora parasitica), potato and tomato late blight (Phytophthora infestans), Vegetables in Brassica clubroot (Plasmodiophora brassicae), the black shin of tobacco Sick (Phytophthora parasitica var.nicotianae), capsicum epidemic disease (Phytophthora capsic) watch Plant corruption mildew (Pythium splendens), lawn rot index (Pythium aphanidermatum), wild cabbage is dampinged off Sick (Pythium aphanidermatum), citrus bacterial canker disease (Xanthomonas campestris), wildfire (Pseudomonas syringae pv.tabaci), tobacco, tomato and eggplant bacterial wilt (Pseudomonas Solanacearum), cucumber bacterial angular leaf spot (Pseudomonas syringae pv.lachrymans), the soft corruption of Chinese cabbage Sick (Erwinia carotovora), beet cercospora leaf spot (Cercospora beticola), tomato, capsicum and potato early epidemic Sick (Alternaria solani), cucumber anthracnose (Colletotrichum orbiculare), citrus and pepper anthracnose (Colletotrichum glecosporioides), apple, strawberry and bitter rot or anthracnose of grape (Glomerella cingulata), Banana anthracnose (Colletotrichum musae), the leaf blight of corn (Exserohilum turcicum), base rot disease (Fusarium graminearum), helminthosporium maydis (Bipolaris maydis), sigatoka (Mycosphaerella Fijiensis), cucurbitaceae vegetable (cucumber, cucurbita pepo, pumpkin) powdery mildew (Sphaerotheca fuliginea), strawberry is white Powder disease (Sphaerotheca aphanis), grapevine anthracnose (Sphaceloma ampelinum), powdery mildew (Erysiphe Necator), white rot (Coniothyrium diptodictta), peanut sclerotium rolfsii (Sclerotium rolfsii), tikka Sick (Cercospora arachidicola), crown rot (Aspergillus niger), cotton verticillium wilt (Verticillium Dahliae), soybean rust (Phakopsora pachyrhizi), purple spot (Cercospora kikuchii), watermelon is climing withered Sick (Mycosphaerella melonis), Alternaria alternate (Alternaria alternata), black scurf of potato and water The sheath and culm blight of rice (Rhizoctonia solani), potato silver maize ear rot (Helminthosporium solani), ornamental flower is black Pinta (Septoria fhrysanth), diseases and insect pests of edible mushroom (such as brown rot Mycogone perniciosa, brown spot Verticillium fungicola, green mold Trichoderma viride, Neurospora Neurospora crassa etc.), peach Bleeding disease (Botryosphaeria ribis), the mould leaf blight (Monographella nivalis) of wheat snow, septoria musiva leaf are withered Sick (Septoria tritici), powdery mildew (Blumeria graminis), full rot (Gaeumannomyces Graminis), leaf rust (Puccinia recondita), stripe rust (P.striiformis), black rust (P.graminis), banded sclerotial blight (Rhizoctonia cerealis), head blight (Fusarium graminearum), root rot (Bipolaris sorokiniana), beans, alfalfa, bulb class, oil crops, sorghum, beet, sunflower, corn, The crops such as cotton samping off and damping-off (Rhizoctonia solani), watermelon and cotton wilt, root rot (Fusarium oxysporum), the crops such as fruit tree, vegetables gray mold (Botrytis cinerea), rape, sunflower sclerotium Sick (Sclerotinia sclerotiorum), rape balck shank (Phoma lingam) etc..

In order to solve the above-mentioned technical problem, the present invention is realised by adopting the following technical scheme：

The present composition component A and B can be with arbitrary proportion mixtures, and a kind of usual active component content is higher than rest activity Ingredient, preferably mixing ratio are 100：1 to 1：100 or 50：1 to 1：50.

The present composition can be by the way that active constituent be mixed with the auxiliary agent that can be used in Pesticide formulation, with known Method is prepared as conventional preparation, such as missible oil, suspending agent, water dispersible granules, aqueous emulsion, microemulsion, wettable powder, particle Agent, dispersible oil-suspending agent, microcapsule suspending agent, suspension emulsion etc..The active constituent of 0.1~95% weight is typically contained in preparation, It is preferred that the active constituent of 0.5~90% weight.

The auxiliary agent that can be used in Pesticide formulation includes but not limited to：Water, solvent, filler, various surfactants (emulsifier, dispersant, wetting agent etc.), adhesive, colorant, antifreezing agent, thickener, suspending agent, disintegrant, disappear at film forming agent Infusion, bleeding agent, warning coloration, stabilizer, wall capsule material, pH adjusting agent, preservative, synergist etc..Also, suitably, in order to It improves to specific crop tolerance, safener can be properly added；It, can be in mixture group or sometimes for crop growth is promoted It closes and conventional agriculture fertilizer is added in object, medicament-fertilizer mixture is made.These auxiliary agents be all commonly use in pesticidal preparations or allow to use into Point, it is not particularly limited, one or more auxiliary agent may be selected and form, specific ingredient and dosage pass through letter according to recipe requirements Single experiment determines.

The production technology of the various application forms of composition belongs to existing known technology, and details are not described herein.

The present composition can use in a variety of ways, be such as watered spraying in a usual manner and use or directly spread fertilizer over the fields or ditch Pesticide-clay mixture is applied or mixes to spread fertilizer over the fields or seed treatment etc., when plant seeding, before emergence, vegetative growth phase and reproductive stage after emergence It can be used.Dosage can change in wider range, and depending on the situation of soil, application method, crop, to be prevented Plant disease type and seedling age size, weather conditions at that time and other factors.The present composition usually with 0.001~ The dosage application of 1.5kg mixture of active principles/hectare is applied with the dosage of 1~1500mg mixture of active principles/kg With；It is further preferable that it is applied with the dosage of 0.02~0.8kg mixture of active principles/hectare, with 20~800mg active constituents The dosage application of mixture/kg.

Compared with prior art, the present invention it has the following technical effect that：

1st, mixture composition has good synergistic effect, and application effect is substantially better than single dose theoretical effect summation, that is, has There is super superposition, can preferably control plant disease occurrence injury；

2nd, there is good complementarity in control of plant disease spectrum between mixture composition active constituent, can expands very well Prevention spectrum, synthesis effectively control various plant disease occurrence injuries；

3rd, mixture composition has good synergistic effect, can reduce pharmaceutical use, reduces use cost and environment is dirty Dye, improves the safety to crop.

Specific embodiment

Specific embodiments of the present invention are given below, it should be noted that the invention is not limited in implement in detail below Example, all equivalents done on the basis of technical scheme each fall within protection scope of the present invention.

Embodiment 1：65% missible oil

Formula composition is：Active components A is 52%, and active constituent B is 13%, and emulsifier styryl phenol polyethenoxy gathers Oxypropylene ether 4%, emulsifier calcium dodecyl benzene sulfonate 3%, solvent xylene complements to 100%.

Preparation method is：All materials are put into batching kettle, stirring and dissolving is to fully transparent, after chemical examination is qualified, transfer It is filling to storage tank.

Embodiment 2：36% suspending agent

Formula composition is：Active components A is 4%, and active constituent B is 32%, wetting agent sulfosuccinic acid monooctyl ester sodium salt 5%, dispersant sodium lignin sulfonate 3%, antifreeze glycol 5%, thickener aluminium-magnesium silicate 0.3%, water complements to 100%.

Preparation method is：By in active constituent and wetting agent, dispersant, antifreezing agent and water input stirred tank, it is sufficiently stirred Afterwards, material is pumped into sand mill and be fully ground, after the completion of grinding, is pumped into high-speed shearing machine, after adding in thickener, into Row high speed shear obtains suspending agent after the completion of shearing.

Embodiment 3：77% water dispersible granules

Formula composition is：Active components A is 66%, and active constituent B is 11%, wetting agent lauryl sodium sulfate 7%, point Powder to methoxyl group fatty acyl amido benzene sulfonic acid sodium salt 5%, fill out by disintegrant ammonium sulfate 4%, adhesive carboxymethyl cellulose 0.2% Material diatomite complements to 100%.

Preparation method is：By all materials after mixing, it crushes through airslide disintegrating mill, is uniformly mixed again, then, add Enter a certain amount of water to mediate this mixture, extruder grain, through dry screening to get to water dispersible granules.

Embodiment 4：18% aqueous emulsion

Formula composition is：Active components A is 6%, and active constituent B is 12%, solvent xylene 10%, solvent Solvesso 1005%, emulsifier castor oil polyoxyethylene ether 5%, emulsifier sorbitan ester 4%, antifreezing agent propylene glycol 4%, water is supplied To 100%.

Preparation method is：After active components A and B are fully dissolved with solvent, input emulsifier forms oil after being sufficiently stirred Phase；Antifreezing agent is added to the water dissolving, forms water phase；Water phase is slowly added in oil phase, is sheared using high-speed shearing machine, you can Obtain aqueous emulsion.

Embodiment 5：14% microemulsion

Formula composition is：Active components A is 7%, and active constituent B is 7%, solvent xylene 10%, solvent cyclohexanone 4%, emulsifier polyoxyethylene sorbitan fatty acid ketone 8%, emulsifier alkylaryl polyoxyethylene polyoxypropylene ether again 7%, Antifreezing agent glycerine 4%, water complements to 100%.

Preparation method is：After active components A and B are fully dissolved with solvent, put into emulsifier and antifreezing agent be uniformly mixed, Deionized water is eventually adding, carries out high speed shear, you can obtains microemulsion.

Embodiment 6：81% wettable powder

Formula composition is：Active components A is 72%, and active constituent B is 9%, wetting agent dialkyl succinylsuccinate sulfonated ester 6%, three phenethyl phenol polyoxyethylene ether phosphate 4% of dispersant, filler attapulgite complements to 100%.

Preparation method is：Active constituent and each auxiliary agent are uniformly mixed, puts into mechanical disintegrating machine and just smash, later It is crushed through airslide disintegrating mill, remixes uniformly, obtain wettable powder.

Embodiment 7：3% granule

Formula composition is：Active components A is 2%, and active constituent B is 1%, wetting agent sldium lauryl sulfate 0.8%, dispersion Agent sodium methylene bis-naphthalene sulfonate 0.6%, colorant carbon black 0.2%, filler bentonite complement to 100%.

Preparation method is：By all materials after mixing, it adds in a certain amount of water to mediate this mixture, extruding is made Grain, through dry screening to get to granule.

Embodiment 8：42% dispersible oil-suspending agent

Formula composition is：Active components A is 6%, and active constituent B is 36%, emulsifier alkyl phenol polyoxyethylene ether formaldehyde Condensation product 7%, emulsifier aerosol-OT salt 6%, dispersant polycarboxylate 2%, thickener white carbon 1.5%, Decentralized medium castor oil complements to 100%.

Preparation method is：By in active constituent and emulsifier, dispersant and decentralized medium input stirred tank, it is sufficiently stirred Afterwards, it is pumped into high-speed shearing machine, carries out high speed shear, then pump and be fully sanded into sand mill, add in and increase after sand milling is qualified It thick dose, stirs evenly up to dispersible oil-suspending agent.

Embodiment 9：25% microcapsule suspending agent

Formula composition is：Active components A is 5%, and active constituent B is 20%, solvent xylene 10%, solvent O-phthalic Diethyl phthalate 6%, oiliness capsule skin 4,4- methyl diphenylene diisocyanates 5%, aqueous capsule skin ethylenediamine 3%, emulsifier is polynary Alcohol fatty acid ester ethylene oxide adduct 8%, dispersant EO-PO block polyethers 6%, thickener xanthans 0.2%, decentralized medium Water complements to 100%.

Preparation method is：After active components A and B are fully dissolved with solvent, oiliness capsule skin is added in, is sufficiently stirred to form oil Phase；It is poured into the water phase for being mixed with emulsifier and dispersant, carries out high speed shear, then add in aqueous capsule skin, stir evenly, It then raises temperature to 70 DEG C of heat preservation solidifications 2 hours, microcapsules is made, are eventually adding thickener, stir evenly up to microcapsule suspending agent.

Embodiment 10：55% suspension emulsion

Formula composition is：Active components A is 50%, and active constituent B is 5%, solvent xylene 10%, emulsifier alkyl phenol Formaldehyde resin polyoxyethylene ether 7%, wetting agent alkylphenol ethoxylate 3%, dispersant naphthalenesulfonic acid-formaldehyde condensate 5%, Antifreezing agent propylene glycol 5%, thickener xanthans 0.3%, water complements to 100%.

Preparation method is：Active components A, wetting agent, dispersant, antifreezing agent and water are put into stirred tank, are sufficiently stirred Afterwards, material is pumped into sand mill and be fully ground, suspending agent is made；After active constituent B is fully dissolved with solvent with breast Agent is uniformly mixed, and is then added to it in Manufactured suspending agent under the conditions of high speed shear, adds in thickener, is carried out high Speed shearing, suspension emulsion is obtained after the completion of shearing.

Application effect example：

1st, test method

(1) to the bioactivity of wheat powdery mildew

Reference《The 4th part of NYT 1156.4-2006 farm-chemical indoor determination test rules fungicide：Prevent white wheat Powder disease tests pot-culture method》It carries out.

It is for examination germ with wheat powdery mildew (Blumeria graminis), laboratory is seeded on fresh wheat seedling Expand numerous spare.

It is for studying object with wheat susceptible variety " Zheng wheat 9023 ", broadcasts 20 seeds per basin, wherein 10 plants are selected after emergence, Length is spare to 2 leaves~3 leaf phases.

Prepared liquid is uniformly sprayed on " Zheng wheat 9023 " blade face to whole wettings.Pathogen is inoculated with after dispenser for 24 hours. It chooses and expands numerous with incidence of leaf, the wheat powdery mildew Fresh spores of interior generation for 24 hours were uniformly shaken off to the leaf phase of 2 leaves~3 and have been applied On the wheat seedling of medicine.Then 18 DEG C, humidity 70%, light application time 16h, interlunation be 8h under the conditions of constant incubator Interior culture.Often processing sets 4 repetitions, often repeatedly 3 basin, and set the processing without medicament and make blank control.When blank control is sent out When sick rate reaches more than 80%, incidence is managed in classification investigation everywhere.Calculate the disease index and control effect of each processing.

Disease index is calculated by formula (1), and control effect is calculated by formula (2), and result of calculation retains 2 significant digits.

In formula：

X-disease index；

N_i- sick the number of sheets at different levels；

I-opposite value of series；

N-investigation total number of sheets；

9-highest value of series.

In formula：

P-control effect, unit are percentage (%)；

CK-blank control disease index；

PT-chemicals treatment disease index.

(2) bioactivity of Phytophthora infestans, apple black star bacteria, Botryosphaeria berengeriana f. sp and tomato early blight bacterium

Reference《NYT 1156.2-2006 farm-chemical indoor determination test rule fungicide part 2s：Inhibit cause of disease true Bacterium mycelia growth test Plating》It carries out.

With phytophthora infestans (Phytophthora infestans), apple black star bacteria (Venturia Inaequalis), Botryosphaeria berengeriana f. sp (Physalospora piricola), tomato early blight bacterium (Alternaria Solani it is) for examination pathogen.

Incidence tissue is acquired from field, laboratory isolates and purifies, is seeded on culture medium and cultivates, cultivate to colony diameter and reach 5cm~6cm or so is spare.Phytophthora infestans oat medium culture, Botryosphaeria berengeriana f. sp, tomato early blight bacterium are used PDA culture medium culture, apple black star bacteria fructus hordei germinatus juice PDA culture medium culture.

Oat medium formula：Oat 50g, agar powder 18g, deionized water 1000mL.

PDA culture medium is laboratory conventional medium, omits its formula herein.

Fructus hordei germinatus juice PDA culture medium formula：1% fructus hordei germinatus juice is added in i.e. in conventional PDA culture medium.

It is spare after the sterilizings such as culture medium, deionized water, scribing line triangular flask, culture dish, pipette tips, card punch, inoculator.

Under aseptic technique, the sterilising medium for advance thawing being cooled to about 50 DEG C is quantitatively adding sterilizing scribing line three In the bottle of angle, make culture medium concordant with (60mL) at scribing line, then draw 600 μ L liquids successively from low concentration to high concentration, respectively It adds in above-mentioned triangular flask, fully shakes up, rear equivalent is poured into 4 culture dishes (Φ 9cm), and 5 concentration gradient band medicines are made and put down Plate.And it sets the processing without medicament and makees blank control.It will cultured pathogen, aseptically use sterilizing on culture medium Card punch (Φ 5mm) cuts bacteria cake from the same circumference of colony edge, with inoculator by pure culture biscuits involvng inoculation in drug containing tablet center, bacterium Silk down, covers ware lid, in incubator dark culturing (phytophthora infestans and apple black star bacteria are cultivated in 20 DEG C, Remaining germ is cultivated in 25 DEG C).According to mycelia growing state in blank control ware, with calliper colony diameter.Each bacterium colony Using crossing method vertical measurement, its average value is taken, calculates the inhibiting rate grown to mycelia.

The inhibiting rate that each concentration for the treatment of grows mycelia is calculated by formula (3), (4), unit is percentage (%), calculates knot Fruit retains 2 significant digits.

D=D₁-D₂…………………………………………(3)

In formula：

D-bacterium colony increases diameter, and unit is millimeter (mm)；

D₁- colony diameter, unit are millimeter (mm)；

D₂- bacteria cake diameter, unit are millimeter (mm).

In formula：

I-mycelial growth inhibition rate, unit are percentage (%)；

D₀- blank control bacterium colony increases diameter, and unit is millimeter (mm)；

D_t- chemicals treatment bacterium colony increases diameter, and unit is millimeter (mm).

(3) to the bioactivity of Colletotrichum capsici

Bibliography is reported, biological activity determination is carried out with pot-culture method.Specific method is as follows：

It is for examination pathogen with Colletotrichum capsici (Colletotrichum glecosporioides), field acquires, Laboratory isolates and purifies, and is seeded in PDA culture medium and cultivates, spare after a large amount of conidiums to be generated.

It is for studying object, being cultivated in controllable heliogreenhouse spare to 4~5 leaf phases with capsicum susceptible variety " line green pepper No. 6 ".

Prepared liquid is uniformly sprayed on " line green pepper No. 6 " blade face to whole wettings.Dispenser is inoculated with pathogen afterwards for 24 hours. It is washed from PDA bacterium colonies with sterile water and takes conidium, cleaning solution is filtered with double gauze, is made a concentration of 1 × 10⁵A spore/ The spore suspension of mL.With pipettor pipette 10 μ L spore suspensions be inoculated in Pepper Leaves front (Colletotrichum capsici is easily invaded Contaminate face of blade), each 2 points of blade inoculation.(relative humidity 95%~100%) dark culturing is moved in humidistat after inoculation 24h.Then it is cultivated in (25 ± 1) DEG C, the growth cabinet that intensity of illumination 20000Lux, relative humidity are 80%~90%. Often processing sets 4 repetitions, often repeatedly 3 basin, and set the processing without medicament and make blank control.

When blank control incidence is up to more than 50%, lesion diameter is measured with crossing method with vernier caliper, is made even Mean value.

According to survey data, control effect is calculated by formula (5), as a result retains 2 significant digits.

In formula：

P-control effect, unit are percentage (%)；

D₀- blank control lesion diameter, unit are millimeter (mm)；

D₁- chemicals treatment lesion diameter, unit are millimeter (mm).

(4) to the bioactivity of citrus processing

Reference《The 16th part of NY/T 1156.16-2008 farm-chemical indoor determination test rules fungicide：It inhibits bacteria Increment tests turbidity method》It carries out.

It is for examination germ with citrus processing (Xanthomonas campestris), divides from the incidence tissue of field From, purifying it is spare.

NA culture medium prescriptions：Polyprotein peptone 5g, dusty yeast 1g, beef extract 3g, sucrose 15g, agar 17g, deionized water 1000mL is adjusted to pH7.0 with the NaOH of 10mol/L.

NB Liquid Culture based formulas：Peptone containing polyprotein 5g, dusty yeast 1g, beef extract 3g, sucrose 15g, deionized water 1000mL is adjusted to pH7.0 with the NaOH of 10mol/L.

Prepared liquid medicine quantitative is added in sterilizing NB culture mediums after cooling, is then put down production in NA culture mediums Strain on plate is diluted to 1 × 10 with aqua sterilisa⁷The bacteria suspension of a spore/mL concentration connects respectively into each processing NB culture mediums Kind 100uL bacterium solutions, shaken cultivation under the conditions of being placed in 28~30 DEG C, rotating speed 120r/min.4 repetitions are often handled, and sets and is free of Blank control is made in the processing of medicament.The turbidity of each processing is measured respectively before starting culture, treats that control treatment reaches logarithmic growth During the phase, the turbidity of each processing is measured and recorded.

According to investigation result, the growth inhibition ratio of bacterium is calculated by formula (6), unit is percentage (%), result of calculation Retain 2 significant digits.

In formula：

R-growth inhibition ratio；

A₀- blank control turbidity value added；

A₁- chemicals treatment turbidity value added.

(5) to the bioactivity of Plasmopara viticola

Bibliography is reported, biological activity determination is carried out with leaf disk method.Specific method is as follows：

It is for examination germ with Plasmopara viticola (Plasmopara viticola), laboratory is seeded in fresh grape leaf On piece expands numerous spare.

Using grape susceptible variety " the red earth " grape as studying object, leaf in grape young sprout apex Section 3 or Section 4 is acquired The basically identical healthy leaves of age, size, the leaf dish that grape leave is broken into a diameter of 1.5cm with card punch are spare.

The leaf dish back side is placed on the turntable of metered dose tower (it is 1 group often to repeat 15 leaf dishes) upward, using level pressure 1.0kg/cm², liquid 10mL, timing 1min make droplet uniform deposition at the leaf dish back side.After liquid natural air drying, by leaf dish It is spare that the back side is placed on moisturizing in the culture dish for be covered with blotting paper upward.It is inoculated with germ afterwards for 24 hours.It chooses and expands numerous incidence of leaf, with 4 DEG C distilled water elutes Plasmopara viticola sporangium from vacuum side of blade, is filtered with double gauze, is made a concentration of 1 × 10⁵A spore The sporangia suspension of ascus/mL, (Pseudoperonospora cubensis easily infects the blade back of the body to the leaf dish back side after being inoculated in chemicals treatment with pipettor Face), each leaf dish is inoculated with 10 μ L sporangia suspensions.Then under the conditions of 17~22 DEG C, 12h alternation of light and darkness, relative humidity is It is cultivated under conditions of more than 90%.4 repetitions of often processing setting, repeat 15 leaf dishes every time, and set the processing without medicament and make Blank control.When blank control incidence reaches more than 80%, it is classified incidence of investigating.Stage division reference《GB/ The field control effectiveness test criterion of T 17980.122-2004 bactericidal agent for preventing and treating downys mildew of garpe》It carries out.Calculate the state of an illness of each processing Index and control effect.

Disease index is calculated by formula (1), and control effect is calculated by formula (2), and result of calculation retains 2 significant digits.

(6) to the bioactivity of cucumber powdery mildew's pathogen

Reference《The 11st part of NY/T 1156.11-2008 farm-chemical indoor determination test rules fungicide：It prevents melon Powdery mildew experiment basin cuts out method》It carries out.

It is for examination germ with cucumber powdery mildew's pathogen (Sphaerotheca fuliginea), is adopted from the cucumber disease leaf of field Collection, indoor inoculation expands numerous spare on fresh cucumber seedling.

It is for studying object, laboratory potting with cucumber susceptible variety " the close thorn in Xintai City ", seedling is grown to 2~4 leaf periods It is spare.

Prepared liquid is uniformly sprayed on " the close thorn in Xintai City " blade face to whole wettings.Pathogen is inoculated with after dispenser 72h. It chooses and expands numerous incidence of leaf, with the pure water added with a small amount of 0.1% Tween-80, Fresh spores, eluent are eluted from sick leaf It is filtered with double gauze, is made a concentration of 1 × 10⁵The spore suspension of a spore/mL, by its spray inoculation in the Huang of dispenser On melon blade.(relative humidity 70%~80%) is moved in humidistat after inoculation, and dark culturing is for 24 hours.It is handed over afterwards in 20 DEG C, 12h brightness It is cultivated under the conditions of replacing.Often processing sets 4 repetitions, often repeatedly 5 basin, every 2 plants of basin, and set the processing without medicament and make blank pair According to.When blank control disease leaf rate reaches more than 80%, incidence is managed in classification investigation everywhere.Calculate the disease index of each processing And control effect.

Disease index is calculated by formula (7), and control effect is calculated by formula (2), and result of calculation retains 2 significant digits.

In formula：

X-disease index；

N_i- sick the number of sheets at different levels；

I-opposite value of series；

N-investigation total number of sheets；

11-highest value of series.

(7) compositions contemplated activity judges

Compositions contemplated activity, which is used for reference in the transnational agricultural chemicals enterprise formulation patents such as Bayer, Syngenta, Tao Shi, judges composition The method (COLBY methods) of expection activity：COLBY, S.R.：“Calculating Synergistic and Antagonistic Responses of Herbicide Combinations ", Weeds 15, p.20~22,1967 carry out.

Two-spot compositions contemplated activity is calculated by (8) formula：

In formula：

Preventive effect or inhibiting rate when 1 dosage of X-medicament is P；

Preventive effect or inhibiting rate when 2 dosage of Y-medicament is Q；

The reality of practical preventive effect or inhibiting rate, i.e. mixture composition after E-medicament 1 is mixed according to the above ratio with medicament 2 Activity；

E₀The theoretical preventive effect or the expection of inhibiting rate, i.e. mixture composition that 1 dosage of-medicament is 2 dosage of P+ medicaments when being Q Activity.

As E ＞ E₀When, i.e., if the substantial activity of Mixed Pharmacy is more than calculated expection activity, the composition just has There is super superposition, that is, act synergistically.

Ternary composition expection activity is calculated by (9) formula：

In formula：

Preventive effect or inhibiting rate when 1 dosage of X-medicament is P；

Preventive effect or inhibiting rate when 2 dosage of Y-medicament is Q；

Preventive effect or inhibiting rate when 3 dosage of Z-medicament is R；

E₀The theoretical preventive effect or inhibiting rate that 1 dosage of-medicament is 2 dosage of P+ medicaments when be 3 dosage of Q+ medicaments being R, that is, combine The expection activity of object；

The substantial activity of practical preventive effect or inhibiting rate, i.e. composition when E-medicament 1,2 and 3 is by different ratio mixture；When E ＞ E₀When, i.e., if the substantial activity of Mixed Pharmacy is more than calculated expection activity, just there is the composition super superposition to make With acting synergistically.

2nd, result of the test

Result of the test refers to table 1~9, it is seen then that active components A (dithianon) with selected from active constituent B (quinoxyfen, Fenamidone, the third oxygen quinoline, sad mantoquita and copper 8-hydroxyquinolinate) medicament mixture after, substantial activity is all higher than expection activity, illustrates two Cyanogen anthraquinone has synergistic effect with the medicament mixture in active constituent B.

1 dithianon of table is with quinoxyfen mixture to the synergy effect of wheat powdery mildew

The synergy effect of 2 dithianon of table and Fenamidone mixture Phytophthora infestans

3 dithianon of table is with the third oxygen quinoline mixture to the synergy effect of Colletotrichum capsici

4 dithianon of table is with sad mantoquita mixture to the synergy effect of apple black star bacteria

5 dithianon of table is with copper 8-hydroxyquinolinate mixture to the synergy effect of citrus processing

6 dithianon of table is with quinoxyfen mixture to the synergy effect of Botryosphaeria berengeriana f. sp

7 dithianon of table, the third oxygen quinoline are with sad mantoquita mixture to the synergy effect of tomato early blight bacterium

8 dithianon of table, Fenamidone and quinoxyfen mixture are to the synergy effect of Plasmopara viticola

9 dithianon of table, copper 8-hydroxyquinolinate and the third oxygen quinoline mixture are to the synergy effect of cucumber powdery mildew's pathogen

Claims (5)

1. bactericidal composition, which is characterized in that including active components A and B.

2. bactericidal composition as described in claim 1, which is characterized in that the active components A is dithianon.

3. bactericidal composition as described in claim 1, which is characterized in that the active constituent B is Fenamidone, benzene oxygen quinoline One or more kinds of combinations in quinoline, the third oxygen quinoline, copper 8-hydroxyquinolinate, sad mantoquita.

4. the bactericidal composition as described in claim 1,2 or 3, which is characterized in that other than active components A and B, further include agriculture The auxiliary agent that can be used in medicine preparation processing.

5. the bactericidal composition as described in claim 1,2,3 or 4, which is characterized in that be applied to controlling plant diseases.