CN108142598A - The culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain - Google Patents
The culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain Download PDFInfo
- Publication number
- CN108142598A CN108142598A CN201810006859.1A CN201810006859A CN108142598A CN 108142598 A CN108142598 A CN 108142598A CN 201810006859 A CN201810006859 A CN 201810006859A CN 108142598 A CN108142598 A CN 108142598A
- Authority
- CN
- China
- Prior art keywords
- cortex eucommiae
- brick tea
- folium cortex
- tea
- coronoid process
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Medicines Containing Plant Substances (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of culture inoculation methods of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, Cortex Eucommiae culture solution will be obtained after folium cortex eucommiae water logging, the coronoid process dissipate capsule bacterium spore obtained from Fu-brick tea is inoculated in Cortex Eucommiae culture solution, and after coronoid process dissipate capsule bacterium is gradually tamed, folium cortex eucommiae Fu-brick tea inoculation liquid is prepared, is seeded in folium cortex eucommiae Fu-brick tea embryo material.Folium cortex eucommiae Fu-brick tea prepared by the present invention does not contain any tea component, but it is prepared using pure Folium Eucommiae according to Fu tea techniques, using technological processes such as decatize, pile fermentation, floatings, bitter taste, astringent taste and the careless fishy smell of folium cortex eucommiae are effectively removed, improve the mouthfeel of Eucommia Tea and drink, simultaneously because employing the coronoid process dissipate capsule bacterium spore inoculating in Fu tea techniques, the aid digestion and weight losing function that folium cortex eucommiae Fu-brick tea has more is added.Folium cortex eucommiae Fu-brick tea integrates the distinctive faint scent of folium cortex eucommiae and coronoid process dissipate capsule bacterium bacterium perfume, in terms of the endoplasms such as fragrance, flavour, soup look more preferably.
Description
Technical field
The invention belongs to tea processing field, and in particular to a kind of coronoid process dissipate capsule bacterium prepared using folium cortex eucommiae in Fu-brick tea
Culture and inoculation method.
Background technology
Fu-brick tea category black tea, also referred to as fermented tea first ancestor have aid digestion weight-reducing and supplement each biostearin, trace element
Deng.First piece of Fu-brick tea, which is recorded, according to history records is born in Xianyang Jingyang County.The Fu-brick tea produced at present, shape brick face is smooth,
Corner angle are clearly demarcated, and thickness is consistent, and floating is generally luxuriant, and Fu brick teas face is dark brown or yellowish-brown.During bubble drink, it is desirable that Tang Hong is not turbid,
Fragrant clear not thick, rich not puckery, mouth strength is strong, endures repeated infusions, and specially requires that coronoid process dissipate capsule bacterium in brick is generally luxuriant, it is dry smell have it is colored clear
It is fragrant.With this coronoid process dissipate capsule bacterium classes of golden flower Fu tea bubble drink when, the fragrance of a flower can be incorporated among millet paste, be turned into tea flavour and more
Mellow micro-puckery, it is pure it is not thick, mouthfeel is powerful.In addition golden flower can also be effectively facilitated adjusting metabolism, reducing blood lipid, conditioning intestines
Stomach plays health care and pathology prevention effect.
Contain lactones, coumarin, alkaloid, polysaccharide, reduced sugar, organic acid, protein, amino acid and Cortex Eucommiae in folium cortex eucommiae
The ingredients such as glue, wherein active ingredient have：Pinoresinol diglucoside, Aucubin, chlorogenic acid, gutta-percha, ajugoside, Du
Secondary glucoside, Kaempferol, caffeic acid, tartaric acid, reduced sugar, chromocor compound, alkaloid etc., have blood pressure lowering, weight-reducing, anti-aging,
Filling liver kidney and other effects.There are Eucommia Tea and series beverage now on the market at present, wherein Eucommia Tea is finished using conventional green tea
Stir-frying technique is made, and is the problem of industry is applied：Folium cortex eucommiae has careless fishy smell, bitter taste and astringent taste in itself so that existing
Some Eucommia Teas and the drink that is prepared according to folium cortex eucommiae the generally existing grass fishy smell in mouthfeel the defects of, limit Eucommia Tea with
And the popularization and application of related folium cortex eucommiae beverage Industry.And at present generally use in folium cortex eucommiae process add in ozone or its
His chemical substance is made a return journey the method for weeding fishy smell, ineffective and complex process, of high cost, is limited it and is widely applied.
Invention content
The purpose of the present invention is to provide a kind of cultures of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain to connect
Kind of method is inoculated with the folium cortex eucommiae Fu-brick tea embryo material after pile fermentation using the inoculation liquid that the program obtains, by Fu tea zymotechniques,
The original astringent taste of folium cortex eucommiae and bitter taste are eliminated, the folium cortex eucommiae Fu-brick tea and its beverage mouthfeel prepared is better than common Cortex Eucommiae
Tea and its drink.
The technical solution adopted by the present invention is as follows：
The culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, it is characterised in that：In Fu bricks
Yellow cleistothecium is selected on tea, bacteria suspension is made after purified and culture；Cortex Eucommiae culture solution will be obtained after folium cortex eucommiae water logging, according to
Ratio from less to more is gradually added in into the bacteria suspension, and coronoid process dissipate capsule bacterium is carried out after gradually taming, Du is prepared
Zhong Ye Fu-brick tea inoculation liquids are inoculated into folium cortex eucommiae Fu-brick tea embryo material.
In the culture inoculation method of the above-mentioned folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, including following step
Suddenly：
【2.1】The preparation of Cortex Eucommiae culture solution
Cortex Eucommiae culture solution is prepared by nutritional agents is added in after fresh folium cortex eucommiae or Folium Eucommiae water logging, gives over to spare；
【2.2】Coronoid process dissipate capsule bacterium obtains and purifying
Floating Fu-brick tea best in quality in the market is selected, capsule is closed using the yellow on aseptic inoculation needle picking Fu-brick tea
Shell is inoculated in standard medium, and the yellow cleistothecium of picking wherein animated period obtains preliminary purification as plate streaking, is put
Enter in the aseptic bottle containing sterile water, it is for use that bacteria suspension is made；
【2.3】Coronoid process dissipate capsule bacterium is tamed
To step【2.2】Bacteria suspension according to ratio from less to more, gradually add in step【2.1】Cortex Eucommiae culture
Liquid after often adding in a Cortex Eucommiae culture solution, is cultivated 2-5 days at 25-30 DEG C, carries out coronoid process dissipate capsule bacterium domestication；
【2.4】The preparation of folium cortex eucommiae Fu-brick tea inoculation liquid
By step【2.3】The base fluid containing bacteria suspension and Cortex Eucommiae culture solution after domestication is placed in shake in shaking table and trains
It supports, cultivation temperature is 28 DEG C -30 DEG C, cultivates 15-20 days, obtains final Cortex Eucommiae Fu tea bacterium solutions, prepares and gives birth to as Cortex Eucommiae Fu tea
Inoculation liquid in production is seeded to the folium cortex eucommiae Fu-brick tea embryo material after pile fermentation.
In the culture inoculation method of the above-mentioned folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, step【2.1】Du
Secondary culture solution according to per kilogram Folium Eucommiae adds 20 kilograms of water or the fresh folium cortex eucommiae of per kilogram adds 10 kilograms of water, in water temperature
70-100 DEG C leaches and obtains.
In the culture inoculation method of the above-mentioned folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, step【2.1】In
Nutritional agents be NaCl, NH4NO3, sucrose, MgSO4、K2HPO4, according to NaCl:NH4NO3:Sucrose:MgSO4:K2HPO4:Water is
32:33:20:40:41:1000 weight ratio mixed configuration.
In the culture inoculation method of the above-mentioned folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, step【2.2】In
Standard medium be configured using dark tea slurry fluid, sucrose, agar and water, its pH value is adjusted in configuration as 5-6.
In the culture inoculation method of the above-mentioned folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, step【2.3】Hat
In prominent bulk bacteria domestication, the Cortex Eucommiae culture solution added in for the first time is 0.05-0.1 times of bacteria suspension volume, Du that last time adds in
Secondary culture solution is 10-50 times of bacteria suspension volume, and the number of centre addition is 3-10 times.
In the culture inoculation method of the above-mentioned folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, standard medium
PH value is 5.8.
The advantageous effects that the present invention has are as follows：
1st, the present invention is in the preparation of folium cortex eucommiae Fu-brick tea, using in the bacteria suspension to coronoid process dissipate capsule bacterium, by low concentration to high
Concentration is gradually added into the scheme of Cortex Eucommiae culture solution, realizes the domestication culture of coronoid process dissipate capsule bacterium.Since coronoid process dissipate capsule bacterium is growth
It is made to generate the strain of golden flower made of conventional tealeaves on Fu-brick tea, and the folium cortex eucommiae Fu tea of the present invention is pressed using pure folium cortex eucommiae
It is prepared according to the technique of Fu tea, itself is simultaneously free of any tealeaves composition.It is well known that self-contained chlorogenic acid of folium cortex eucommiae etc.
Antibacterial medicinal component can inhibit the growth of coronoid process dissipate capsule bacterium spore, therefore in order to enable coronoid process dissipate capsule bacterium can be numerous in folium cortex eucommiae
It grows, therefore employs the process for gradually cultivating domestication, according to being gradually added into the culture solution containing Cortex Eucommiae in bacteria suspension so that coronoid process
Bulk bacteria gradually adapt to the environment of Cortex Eucommiae culture solution, finally realize common coronoid process dissipate capsule bacterium spore in pure folium cortex eucommiae Fu-brick tea
On culture and maturation, and floating is luxuriant.
2nd, the folium cortex eucommiae Fu-brick tea and its beverage prepared using present invention process, but pure Folium Eucommiae is used according to Fu tea
Technique is prepared, and using technological processes such as decatize, pile fermentation, floatings, has effectively removed bitter taste, astringent taste and the careless raw meat of folium cortex eucommiae
Taste, improves the mouthfeel of Eucommia Tea and drink, simultaneously because employing the coronoid process dissipate capsule bacterium spore inoculating in Fu tea techniques, more increases
The aid digestion and weight losing function that folium cortex eucommiae Fu-brick tea has is added.
3rd, there is individual style using folium cortex eucommiae Fu-brick tea organoleptic quality prepared by present invention process, folium cortex eucommiae Fu-brick tea is rubbed
It is fragrant to close the distinctive faint scent of folium cortex eucommiae and coronoid process dissipate capsule bacterium bacterium, without astringent taste in terms of the endoplasms such as fragrance, flavour, soup look, soup look is yellowish
Bright, quality in addition to the nutrition for keeping Cortex Eucommiae, while is also the health protection tea of a breeding taste more preferably.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is done with reference to embodiment
It is further to be described in detail.Here, the illustrative embodiments of the present invention and their descriptions are used to explain the present invention, but it is not intended as pair
The restriction of the present invention.
Embodiment 1
The folium cortex eucommiae Fu-brick tea preparation method based on natural strain of the present invention, includes the following steps：
(1) acquisition of folium cortex eucommiae
Pick the folium cortex eucommiae of the annual 7-10 months, natural air drying dries or drying system drying, and water content is 10%, drying
Temperature be 65 DEG C, put with Polywoven Bag be placed in it is spare at aeration-drying.
(2) preliminary treatment of folium cortex eucommiae
Folium cortex eucommiae is cleaned up, with cutter be divided into the directly upper round as a ball screening and choosing impurity elimination of slice or whole leaf to get
Cortex Eucommiae leaf raw material.
(3) preparation of Cortex Eucommiae culture solution
The smalls folium cortex eucommiae that sieve round as a ball in step (2) leaks down is leached into Cortex Eucommiae juice, is added according to per kilogram Folium Eucommiae
20 kilograms of water is obtained in 70-100 DEG C of leaching of water temperature.NaCl, NH are added in leaching liquid4NO3, sucrose, MgSO4、K2HPO4Make
For nutritional agents, and according to NaCl:NH4NO3:Sucrose:MgSO4:K2HPO4:Water is 32:33:20:40:41:1000 weight ratio is mixed
Configuration is closed, Cortex Eucommiae culture solution is made, is given over to follow-up spare.
(4) coronoid process dissipate capsule bacterium is obtained and is purified
Floating Fu-brick tea 3-5 kinds best in quality in the market are selected, using the yellow on aseptic inoculation needle picking Fu-brick tea
Cleistothecium is inoculated in standard medium, is cultivated 2 days at 30 DEG C；The yellow cleistothecium of picking wherein animated period makees plate streaking,
Preliminary purification is obtained after 2 scribing line, is put into the aseptic bottle containing sterile water, it is for use that bacteria suspension is made.Its Plays
Culture medium is configured using dark tea slurry fluid, sucrose, agar and water, its pH value is adjusted in configuration as 5-6, preferably PH=5.8,
Its configuration concentration and method refer to routine techniques document.
(5) coronoid process dissipate capsule bacterium is tamed
In the bacteria suspension of step (4), according to ratio from less to more, the Cortex Eucommiae culture solution of step (3) is gradually added in, according to
The secondary volume ratio according to opportunistic pathogen suspension and Cortex Eucommiae culture solution is respectively 1:0.05、1:0.5、1:2、1:5、1:10 ratio adds in,
It after often adding in a Cortex Eucommiae culture solution, is cultivated 2 days at 25 DEG C, when coronoid process dissipate capsule bacterium growth is vigorous, that is, completes domestication.
(6) preparation of Cortex Eucommiae Fu tea inoculation liquid
The base fluid containing bacteria suspension and Cortex Eucommiae culture solution after step (5) is tamed is placed in shake culture in shaking table, cultivates
Temperature is 28 DEG C, rotating speed 200r/min, cultivates 15 days, obtains final Cortex Eucommiae Fu tea bacterium solutions, prepares and gives birth to as Cortex Eucommiae Fu tea
Inoculation liquid in production.
(7) decatize, pile fermentation
After the processed Cortex Eucommiae leaf raw material water spray of step (2), high temperature steaming when detection water content reaches 20%, decatize temperature
It is 100 DEG C to spend, a length of 5 minutes during decatize；Eucommia ulmoides Oliv. leaves after decatize are accumulated immediately, the processing capacity of foundation folium cortex eucommiae,
Concentrate accumulation.Heap is 2 meters high, pile fermentation time 3.5 hours, and temperature control is at 75 DEG C -80 DEG C in heap.If it should be turned over immediately during more than 80 DEG C
Heap radiates.Folium cortex eucommiae after pile fermentation, Appearance color become the consistent yellowish-brown of color and luster by different variegated of the depth, endoplasm flavour by
Not mature grass fishy smell becomes mellow fragranced.Decatize and pile fermentation make folium cortex eucommiae fully soften, and remove peculiar smell, effectively remove Cortex Eucommiae
The careless fishy smell of leaf, while by physic-chemical changes complicated in hydrothermal promotion folium cortex eucommiae blade.
(8) mixing of folium cortex eucommiae embryo material and Cortex Eucommiae leaf sap
Folium cortex eucommiae after pile fermentation is precisely weighed, after determining folium cortex eucommiae embryo material weight, the Cortex Eucommiae culture of addition step (3)
Liquid, between adjustment Cortex Eucommiae leaf water content 20-40%, (usually according in every 500g folium cortex eucommiaes embryo material plus Cortex Eucommiae culture solution 200g),
It stirs, carries out decatize softening, 100 DEG C of steam temperature again by steam engine, folium cortex eucommiae mixing was made in 5 seconds time
Embryo material.
(9) it is inoculated with coronoid process dissipate capsule bacterium spore
It will uniformly be spread out by the folium cortex eucommiae mixing embryo material that decatize softened, cooled to room temperature softens according to decatize
The weight of folium cortex eucommiae mixing embryo material afterwards, the Cortex Eucommiae Fu tea inoculation liquids of sprinkling step (6), fountain height are inoculated with reference to general Fu tea
Depending on technique.
(10) floating is suppressed
Inoculation coronoid process is dissipated to the folium cortex eucommiae embryo material of cystospore bacterium, mold is packed into after weighing as required, is pressed into tea brick type.
It is placed in progress floating drying, as folium cortex eucommiae Fu-brick tea finished product in drying chamber again.
Embodiment 2
(1) acquisition of folium cortex eucommiae
It picks the 7-10 months without the neat folium cortex eucommiae in insect pest, blade face every year.
(2) preliminary treatment of folium cortex eucommiae
Folium cortex eucommiae is cleaned up, with cutter be divided into the directly upper round as a ball screening and choosing impurity elimination of slice or whole leaf to get
Cortex Eucommiae leaf raw material.
(3) preparation of Cortex Eucommiae culture solution
The smalls folium cortex eucommiae that sieve round as a ball in step (2) leaks down is leached into Cortex Eucommiae juice, is added according to per kilogram Folium Eucommiae
20 kilograms of water is obtained in 70-100 DEG C of leaching of water temperature.NaCl, NH are added in leaching liquid4NO3, sucrose, MgSO4、K2HPO4Make
For nutritional agents, and according to NaCl:NH4NO3:Sucrose:MgSO4:K2HPO4:Water is 32:33:20:40:41:1000 weight ratio is mixed
Configuration is closed, Cortex Eucommiae culture solution is made, is given over to follow-up spare.
(4) coronoid process dissipate capsule bacterium is obtained and is purified
Floating Fu-brick tea 3-5 kinds best in quality in the market are selected, using the yellow on aseptic inoculation needle picking Fu-brick tea
Cleistothecium is inoculated in standard medium, is cultivated 2 days at 30 DEG C；The yellow cleistothecium of picking wherein animated period makees plate streaking,
Preliminary purification is obtained after 2-3 scribing line, is put into the aseptic bottle containing sterile water, it is for use that bacteria suspension is made.It is got the bid
Quasi- culture medium is configured using dark tea slurry fluid, sucrose, agar and water, its pH value is adjusted in configuration as 5-6, preferably PH=
5.8, configuration concentration and method refer to routine techniques document.
(5) coronoid process dissipate capsule bacterium is tamed
In the bacteria suspension of step (4), according to ratio from less to more, the Cortex Eucommiae culture solution of step (3) is gradually added in, according to
The secondary volume ratio according to opportunistic pathogen suspension and Cortex Eucommiae culture solution is respectively 1:0.1、1:0.5、1:2、1:5、1:10、1:50 ratio adds
Enter, after often adding in a Cortex Eucommiae culture solution, cultivated 2 days at 25 DEG C, when coronoid process dissipate capsule bacterium growth is vigorous, that is, complete domestication.
(6) preparation of Cortex Eucommiae Fu tea inoculation liquid
The base fluid containing bacteria suspension and Cortex Eucommiae culture solution after step (5) is tamed is placed in shake culture in shaking table, cultivates
Temperature is 28 DEG C, rotating speed 200r/min, cultivates 20 days, obtains final Cortex Eucommiae Fu tea bacterium solutions, prepares and gives birth to as Cortex Eucommiae Fu tea
Inoculation liquid in production.
(7) decatize, pile fermentation
The processed Cortex Eucommiae leaf raw material of step (2) is subjected to high temperature steaming, steam temperature is 120 DEG C, a length of 1 point during decatize
Clock；Eucommia ulmoides Oliv. leaves after decatize are accumulated immediately, according to the processing capacity of folium cortex eucommiae, concentrate accumulation.Heap is 2 meters high, during pile fermentation
Between 5 hours, in heap temperature control at 75 DEG C -80 DEG C.It if should the heat dissipation of turning immediately during more than 80 DEG C.Folium cortex eucommiae after pile fermentation, outside
Shape and color pool becomes the consistent yellowish-brown of color and luster by different variegated of the depth, and endoplasm flavour becomes mellow fragrance by not mature careless fishy smell
Taste.Decatize and pile fermentation make folium cortex eucommiae fully soften, and remove peculiar smell, effectively remove the careless fishy smell of folium cortex eucommiae, while by hydro-thermal
Effect promotes physic-chemical changes complicated in folium cortex eucommiae blade.
(8) mixing of folium cortex eucommiae embryo material and Cortex Eucommiae leaf sap
Folium cortex eucommiae after pile fermentation is precisely weighed, after determining folium cortex eucommiae embryo material weight, the Cortex Eucommiae culture of addition step (3)
Liquid between adjustment Cortex Eucommiae leaf water content 20-40% (usually according in every 500g folium cortex eucommiaes embryo material plus Cortex Eucommiae culture solution 200g), fills
Divide and stir evenly, carry out decatize softening, 107 DEG C of steam temperature again by steam engine, folium cortex eucommiae mixing embryo was made in 10 seconds time
Material.
(9) it is inoculated with coronoid process dissipate capsule bacterium spore
It will uniformly be spread out by the folium cortex eucommiae mixing embryo material that decatize softened, cooled to room temperature softens according to decatize
The weight of folium cortex eucommiae mixing embryo material afterwards, the Cortex Eucommiae Fu tea inoculation liquids of sprinkling step (6), fountain height are inoculated with reference to general Fu tea
Depending on technique.
(10) floating is suppressed
Inoculation coronoid process is dissipated to the folium cortex eucommiae embryo material of cystospore bacterium, mold is packed into after weighing as required, is pressed into tea brick type.
It is placed in progress floating drying, as folium cortex eucommiae Fu-brick tea finished product in drying chamber again.
Folium cortex eucommiae Fu-brick tea has been prepared according to embodiment 1,2 respectively, and 9 moral standings mirror group is invited to carry out trial test and is commented
Valency, obtains that the results are shown in table below.
| Project name | Organoleptic indicator |
| Floating state and color and luster | It is golden, universal luxuriant |
| Color and luster | Brown butter profit |
| Fragrance | Fragrance and the coronoid process dissipate capsule bacterium bacterium for having Cortex Eucommiae after brewing are fragrant |
| Brew soup look | Huang Liang |
| Flavour | Alcohol and pure, without careless fishy smell |
| Tea residue | Yellowish-brown |
Claims (7)
1. the culture inoculation method of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain, it is characterised in that：In Fu-brick tea
On select yellow cleistothecium, bacteria suspension is made after purified and culture；Will after folium cortex eucommiae water logging obtain Cortex Eucommiae culture solution, according to from
More ratios is arrived less, is gradually added in into the bacteria suspension, and coronoid process dissipate capsule bacterium is carried out after gradually taming, Cortex Eucommiae is prepared
Leaf Fu-brick tea inoculation liquid is inoculated into folium cortex eucommiae Fu-brick tea embryo material.
2. the culture inoculation method of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium according to claim 1 based on natural strain,
It is characterized by comprising the following steps：
【2.1】The preparation of Cortex Eucommiae culture solution
Cortex Eucommiae culture solution is prepared by nutritional agents is added in after fresh folium cortex eucommiae or Folium Eucommiae water logging, gives over to spare；
【2.2】Coronoid process dissipate capsule bacterium obtains and purifying
Floating Fu-brick tea best in quality in the market is selected, using the yellow cleistothecium on aseptic inoculation needle picking Fu-brick tea, is connect
For kind in standard medium, the yellow cleistothecium of picking wherein animated period obtains preliminary purification as plate streaking, is put into and contains
Have in the aseptic bottle of sterile water, it is for use that bacteria suspension is made；
【2.3】Coronoid process dissipate capsule bacterium is tamed
To step【2.2】Bacteria suspension according to ratio from less to more, gradually add in step【2.1】Cortex Eucommiae culture solution, often
It after adding in a Cortex Eucommiae culture solution, is cultivated 2-5 days at 25-30 DEG C, carries out coronoid process dissipate capsule bacterium domestication；
【2.4】The preparation of folium cortex eucommiae Fu-brick tea inoculation liquid
By step【2.3】The base fluid containing bacteria suspension and Cortex Eucommiae culture solution after domestication is placed in shake culture in shaking table, training
It is 28 DEG C -30 DEG C to support temperature, cultivates 15-20 days, obtains final Cortex Eucommiae Fu tea bacterium solutions, prepares in production and connects as Cortex Eucommiae Fu tea
Kind liquid, is seeded to the folium cortex eucommiae Fu-brick tea embryo material after pile fermentation.
3. the culture inoculation method of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium according to claim 1 based on natural strain,
It is characterized in that：Step【2.1】Cortex Eucommiae culture solution adds 20 kilograms of water or fresh Du of per kilogram according to per kilogram Folium Eucommiae
Zhong Ye adds 10 kilograms of water, is obtained in 70-100 DEG C of leaching of water temperature.
4. the culture inoculation method of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium according to claim 1 based on natural strain,
It is characterized in that：Step【2.1】In nutritional agents be NaCl, NH4NO3, sucrose, MgSO4、K2HPO4, according to NaCl:NH4NO3:
Sucrose:MgSO4:K2HPO4:Water is 32:33:20:40:41:1000 weight ratio mixed configuration.
5. the culture inoculation method of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium according to claim 1 based on natural strain,
It is characterized in that：Step【2.2】In standard medium be configured using dark tea slurry fluid, sucrose, agar and water, in configuration
Its pH value is adjusted as 5-6.
6. the culture inoculation method of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium according to claim 1 based on natural strain,
It is characterized in that：Step【2.3】In coronoid process dissipate capsule bacterium domestication, the Cortex Eucommiae culture solution added in for the first time is the 0.05- of bacteria suspension volume
0.1 times, the Cortex Eucommiae culture solution that last time adds in is 10-50 times of bacteria suspension volume, and the number of centre addition is 3-10 times.
7. the culture inoculation method of the folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium according to claim 5 based on natural strain,
It is characterized in that：The pH value of standard medium is 5.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810006859.1A CN108142598A (en) | 2018-01-04 | 2018-01-04 | The culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810006859.1A CN108142598A (en) | 2018-01-04 | 2018-01-04 | The culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108142598A true CN108142598A (en) | 2018-06-12 |
Family
ID=62460696
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810006859.1A Pending CN108142598A (en) | 2018-01-04 | 2018-01-04 | The culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108142598A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114982844A (en) * | 2022-01-24 | 2022-09-02 | 浙江欣诺医药有限公司 | Preparation method of fermented strawberry tea |
| CN116970665A (en) * | 2023-09-22 | 2023-10-31 | 凡可生物技术(广州)有限公司 | Extraction method and application of tremella aurantialba polysaccharide fermented by tremella aurantialba |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03240472A (en) * | 1990-02-19 | 1991-10-25 | Hitachi Zosen Corp | Manufacturing method of semi-fermented Duchu tea |
| CN102084904A (en) * | 2010-12-21 | 2011-06-08 | 湖南城市学院 | Preparation technology for fu brick tea flowering loosing tea |
| CN104286247A (en) * | 2014-09-19 | 2015-01-21 | 陕西科技大学 | Preparation method of detoxified gingko tea |
| CN104738256A (en) * | 2013-12-31 | 2015-07-01 | 深圳华大基因科技有限公司 | Fu tea and preparation method and use thereof |
| CN104982540A (en) * | 2015-07-20 | 2015-10-21 | 刘冬 | Maturative agent for hawk tea golden flower cheese and preparation method of maturative agent |
| CN106234720A (en) * | 2016-09-29 | 2016-12-21 | 郑文利 | A kind of novel Eucommia Tea and preparation method thereof |
| CN107125368A (en) * | 2017-06-06 | 2017-09-05 | 河南科技大学 | A kind of preparation method of the red peony petal tea beverage of Lactobacillus plantarum fermentation phoenix |
-
2018
- 2018-01-04 CN CN201810006859.1A patent/CN108142598A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03240472A (en) * | 1990-02-19 | 1991-10-25 | Hitachi Zosen Corp | Manufacturing method of semi-fermented Duchu tea |
| CN102084904A (en) * | 2010-12-21 | 2011-06-08 | 湖南城市学院 | Preparation technology for fu brick tea flowering loosing tea |
| CN104738256A (en) * | 2013-12-31 | 2015-07-01 | 深圳华大基因科技有限公司 | Fu tea and preparation method and use thereof |
| CN104286247A (en) * | 2014-09-19 | 2015-01-21 | 陕西科技大学 | Preparation method of detoxified gingko tea |
| CN104982540A (en) * | 2015-07-20 | 2015-10-21 | 刘冬 | Maturative agent for hawk tea golden flower cheese and preparation method of maturative agent |
| CN106234720A (en) * | 2016-09-29 | 2016-12-21 | 郑文利 | A kind of novel Eucommia Tea and preparation method thereof |
| CN107125368A (en) * | 2017-06-06 | 2017-09-05 | 河南科技大学 | A kind of preparation method of the red peony petal tea beverage of Lactobacillus plantarum fermentation phoenix |
Non-Patent Citations (4)
| Title |
|---|
| 丁可珍: "《采茶和制茶》", 28 February 1982, 农业出版社 * |
| 熊昌云，等: "人工接种发酵茯砖茶降脂减肥作用研究", 《菌物学报》 * |
| 陈社强: "《黑茶时代》", 30 September 2010, 当代世界出版社 * |
| 黄友谊: "《茶叶微生物产品学》", 31 August 2017, 中国轻工业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114982844A (en) * | 2022-01-24 | 2022-09-02 | 浙江欣诺医药有限公司 | Preparation method of fermented strawberry tea |
| CN116970665A (en) * | 2023-09-22 | 2023-10-31 | 凡可生物技术(广州)有限公司 | Extraction method and application of tremella aurantialba polysaccharide fermented by tremella aurantialba |
| CN116970665B (en) * | 2023-09-22 | 2023-12-19 | 凡可生物技术(广州)有限公司 | Extraction method and application of tremella aurantialba polysaccharide fermented by tremella aurantialba |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102696782B (en) | Processing method of fermented bean curd | |
| CN102960466A (en) | Preparation method of Chinese medicinal herbal fermented beancurd | |
| CN104012668A (en) | Fermented tofu preparation process | |
| CN105053363B (en) | A kind of production method of health-care tea made of mulberry leaves | |
| CN105779206B (en) | Semi-sweet applejack | |
| CN109943438B (en) | A preparation method of red koji health wine added fermented by Trichomonas coronis | |
| CN107951005A (en) | A kind of eucommia bark leaf health-care lozenge and preparation method thereof | |
| KR20120048851A (en) | Manufacturing method of korean traditional rice beer and rice wine using uniquely processed leaves and flowers of white lotus | |
| CN107156666A (en) | A kind of processing technology of natto | |
| CN107980995A (en) | A kind of compound eucommia bark leaf Fu-brick tea health drink and preparation method thereof | |
| CN108142598A (en) | The culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium based on natural strain | |
| CN110699265A (en) | A kind of method and special strain for producing Lycium barbarum leaf tea by fermentation of R. coronoides | |
| CN108283234A (en) | Folium cortex eucommiae Fu-brick tea and preparation method thereof based on natural strain | |
| CN101125019B (en) | Pholiota adiposa mycelium fermentation vinegar beverage and preparation method | |
| CN108913416A (en) | A kind of brewing method of loquat fruit wine | |
| CN1317984C (en) | Glossy ganoderma health vinegar beverage and its prepn process | |
| CN107996785A (en) | A kind of solid-state folium cortex eucommiae Fu brick instant tea and preparation method thereof | |
| JPH0563130B2 (en) | ||
| CN108185241A (en) | A kind of passion fruit and the composite beverage of sugarcane and preparation method thereof | |
| CN105361105B (en) | A kind of production method of soy sauce | |
| CN107586645A (en) | A kind of three grain wine and its brew method | |
| CN108552354A (en) | A kind of culture inoculation method of folium cortex eucommiae Fu-brick tea coronoid process dissipate capsule bacterium | |
| CN108112756A (en) | Compound eucommia bark leaf Fu-brick tea health drink based on natural strain and preparation method thereof | |
| CN107927242A (en) | A kind of folium cortex eucommiae Fu-brick tea and preparation method thereof | |
| CN108552355A (en) | Pure folium cortex eucommiae Fu-brick tea oral solution based on natural strain and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180612 |
|
| RJ01 | Rejection of invention patent application after publication |