CN108077617A - A kind of preparation method of antibacterial peptide pannage - Google Patents
A kind of preparation method of antibacterial peptide pannage Download PDFInfo
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- CN108077617A CN108077617A CN201711315434.0A CN201711315434A CN108077617A CN 108077617 A CN108077617 A CN 108077617A CN 201711315434 A CN201711315434 A CN 201711315434A CN 108077617 A CN108077617 A CN 108077617A
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
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- A23K10/00—Animal feeding-stuffs
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- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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Abstract
The invention discloses a kind of preparation methods of antibacterial peptide pannage, belong to field of fodder.The present invention carries out mixed fermentation by adding probiotics, obtains abundant thick protein, amino acid, mineral trace element and vitamin, decomposes crude fibre therein and supplies animal use for monose or oligosaccharide, can improve palatability, improves protein content；Exogenous enzymes are added by enzymolysis, help the decomposition of nutriment in fermentation raw material, supplement the secretion of the deficiency and activation endogenous enzymes of endogenous enzymes, hydrolyze non-starch polysaccharide anti-nutritional factors, reduce alimentary canal content viscosity, the absorption of nutriment is enhanced, improves metaboilic level, enhances the immunity of porkling；Add antibacterial peptide can it is antimycotic, viral and reduce parasite activity, be not likely to produce drug resistance.The present invention solves at present that common pig's feed palatability is poor, and protein content is low, it is impossible to improve porkling immunity, the problem of Side effect is larger, and security is poor.
Description
Technical field
The invention belongs to field of fodder, and in particular to a kind of preparation method of antibacterial peptide pannage.
Background technology
Microbiological feed is using microorganism, complex enzyme as biological feed fermentation agent strain, and feedstuff is converted into micro- life
Object mycoprotein, bioactive micro peptide amino acid, microbial activity probiotics, complex enzyme formulation are integrated biofermentation feeding
Material.With the continuous improvement of people's living standards, the quality requirement of livestock products is continuously improved, the scale of aquaculture forward direction, standard
Change direction to develop.At present, extensive intensive culture also has many ripe broiler fodders and formula to promote market pig
Rapid growth, but pig flesh flavor declines, and taste is delicious.At present, the addition of antibiotic and hormone is very universal in pannage,
Feed micro-ecological additive is unstable to high temperature, hydrochloric acid in gastric juice and cholate, only a small number of bacterial strains is caused to enter active after enteron aisle, from
And the required number of viable that plays a role is not achieved, in addition also have that some are unstable, shelf-life short these problems, so as to shadow
The growth result of pig is rung.Correct Feed selection is most important, to allow in pig body and normally be developed, grown, prevent from disappearing
Change bad and insufficient to absorption of nutrient ingredients.China's concentrated feed consumption is very big, and the equilibrium of supply and demand is very fragile, high-quality micro- life
Object fermented feed resource critical shortage, is ecological microbial agent based on probiotics, and research and development is quickly in the recent decade.It is various micro-
Ecological agent is widely used in the mankind, animal and plant, has generated significant social effect and economic benefit, has been subject to scientific research department
Door, the extensive concern in relation to enterprise.Pass through the composite bacteria liquid machine that a variety of anaerobics or suspicion oxygen microflora are combined
Carry out solid fermentation after entering to other raw materials, make in fermented product containing it is a large amount of beneficial to viable bacterias and with polysaccharide, polypeptide, organized enzyme,
Nutriment by being added in ferment complete feed and feedstuff, can be decomposed or be converted by the metabolites such as organic acid
It is more easy to the small-molecule substance absorbed.So that market pig, while the speed of growth does not substantially slow down, meat quality is better than general
The market pig of logical forage feed.In its feeding field, in terms of fermentation technique has been applied to feed.Current microbial fermentation
Feed mainly has fermented bean dregs or miscellaneous dregs of rice based on black-koji mould, feed fermentation complete feed in saccharified pig feed technology and bag
Deng, these fermentative feedstuff of microbe all there is it is certain the problem of：As saccharified pig feed technology, belong to solid in nature under natural conditions
There is the spontaneous fermentation of microorganism.Due to region and the difference of weather, along with feedstuff kind, quality and ratio not
Together, the saccharification result for causing feed is multifarious, so as to cause feeding effect poor；And the fermented bean dregs or miscellaneous based on black-koji mould
The dregs of rice belong to fungi fermentation, can generate a certain number of induced enzymes such as protease, amylase, cellulase etc., livestock and poultry is contributed to raise
The digestion of dregs of beans in material.But while digestive ferment is generated, black-koji mould can also supervene certain mycotoxin, when this
Product in feed more than 2% when, livestock and poultry can be harmful to, usage amount is more than that 10% even livestock and poultry can be caused dead, it is impossible to be put safely
Heart is used in animal and fowl fodder.Therefore it is badly in need of a kind of side of the good and safe and reliable fermentative feedstuff of microbe of product feeding effect
Method, to meet the needs of market is for fermentative feedstuff of microbe.
The content of the invention
The technical problems to be solved by the invention：Poor for current common pig's feed palatability, protein content is low,
Porkling immunity cannot be improved, the problem of Side effect is larger, and security is poor, provides a kind of antibacterial peptide pannage
Preparation method.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of antibacterial peptide pannage, which is characterized in that the preparation method includes the following steps：
（1）Lactobacillus plantarum and Bifidobacteria powder is taken to mix, obtains mixing bacterium powder, mixing bacterium powder is seeded to antibacterial peptide seed meat
Soup culture medium, culture, obtains antibacterial peptide zymophyte suspension, and antibacterial peptide fermenting microbe bacteria suspension and fermentation culture are added in and fermented
Tank obtains antibacterial peptide zymotic fluid, and antibacterial peptide fermentate is placed in constant temperature blender with magnetic force, adds in wheat bran, is adsorbed, after finishing
Antibacterial peptide zymotic fluid with wheat bran separate, removes zymotic fluid, collects wheat bran, it is dry to constant weight, obtain dry antibacterial peptide-
Wheat bran compound；
（2）Water is added to mix pannage raw material and fully moisturize mixing, 121 DEG C of sterilizing 30min, raw material after must sterilizing is cooled to room
Temperature adds in mannonase pectase, cellulase, is digested, rocked daily 2 times, feedstuff after must digesting；
（3）Lactic acid bacteria, bacillus subtilis, candida utili bacterium are mixed, fermentation mixed bacteria is obtained, fermenting microbe is mixed
Object is seeded in strain activation and culture base and cultivates, and obtains fermenting microbe suspension, and filtering removes mycelium, takes filtrate, obtains fermenting microbe
Feedstuff after above-mentioned dry antibacterial peptide-wheat bran compound, enzymolysis is added in feed fermentation nutrient solution, must mix feeding by seed
Expect fermentate, fermenting microbe seed liquor is seeded in mixed fodder fermentate by the inoculum concentration of mass fraction 10%, ferment, obtain
Antibacterial peptide pannage fermentate takes antibacterial peptide pannage fermentate to dry, obtains antibacterial peptide pannage.
The step（1）Middle antibacterial peptide producing strains are lactobacillus plantarum and Bifidobacterium in mass ratio 3:2 mixing, antibacterial peptide
The formula of seed broth bouillon is according to the mass fraction, 8 ~ 10 parts of peptone, 1 ~ 2 part of beef extract, 4 ~ 6 parts of NaCl, pH 7.2
± 0.2,121 DEG C of high pressure steam sterilization 20min；The formula of antibacterial peptide fermentation culture is according to the mass fraction, to take sucrose 10 ~ 12
Part, 18 ~ 20 parts of corn flour, CaCl23 ~ 5 parts, ZnCl212 ~ 15 parts, 0.1 ~ 0.6 part of Tween-20,1000 parts of water, pH7.2 ±
0.2,121 DEG C of high pressure steam sterilization 20min.
The step（1）The mass ratio of middle antibacterial peptide producing strains and antibacterial peptide seed broth bouillon is 1:9, antibacterial peptide hair
The mass ratio of yeast-like fungi kind bacteria suspension and fermentation culture is 2:5, fermentation condition is：Fermentation throughput is set as 0.4 ~ 2.0m3/ h,
Rotating speed is 400r/min, dissolved oxygen is 50 ~ 70%, 32 ~ 35 DEG C of fermentation temperature, 14 ~ 18h of fermentation time.
The step（1）The mass ratio of middle wheat bran and antibacterial peptide fermentate is 2:7, adsorb 0.5 ~ 1h.
The step（2）Middle pannage fermentation raw material include fresh grapefruit skin slag, wheat bran, wheat flour, unite chaff, in mass ratio
7:3:2:4 are uniformly mixed.
The step（2）The mass ratio of middle feed fermentation raw material and water is 5:4, mannonase pectase, cellulase
Mass ratio with raw material after sterilizing is 5:4:2:7, the condition of enzymolysis is 40 ~ 45 DEG C of 60 ~ 72h of constant temperature.
The step（3）Middle lactic acid bacteria, bacillus subtilis, the mass ratio of candida utili bacterium are 2:1:1, it is dry anti-
Feedstuff, the mass ratio of feed fermentation nutrient solution are 3 after bacterium peptide-wheat bran compound, enzymolysis:5:12.
The step（3）The formula of middle strain activation and culture base is：According to the mass fraction, 16 ~ 19 parts of yeast extract, albumen are taken
10 ~ 12 parts of peptone, 9 ~ 12 parts of glucose, CaCO31.7 ~ 2.2 parts, 1000 parts of water, 121 DEG C of sterilizing 30min；Feed fermentation nutrient solution
Configuration：It counts in parts by weight, takes 12 ~ 15 parts of corn flour, 20 ~ 24 parts of dregs of beans, 8 ~ 10 parts of beef extract, 6 ~ 9 parts of peptone, grape
13 ~ 17 parts of sugar, 18 ~ 24 parts of wheat bran, MgSO4·7H20.2 ~ 0.5 part of O, FeSO4·7H20.02 ~ 0.03 part of O, CaCO33 ~ 5 parts,
1000 parts of water, stirs evenly.
Compared with other methods, advantageous effects are the present invention：
（1）The present invention carries out mixed fermentation by adding probiotics, and it is micro to obtain abundant thick protein, amino acid, minerals
Element and vitamin decompose crude fibre therein and supply animal use for monose or oligosaccharide, and can improve palatability, improve egg
White matter content；
（2）The present invention helps the decomposition of nutriment in fermentation raw material, supplements endogenous enzymes by adding exogenous enzymes by enzymolysis
（It is primarily referred to as digestive enzymes such as amylase, protease, lipase etc.）Deficiency and activation endogenous enzymes secretion, hydrolyze non-starch
Polysaccharide anti-nutritional factors reduces alimentary canal content viscosity, enhances the absorption of nutriment, improve metaboilic level, enhances
The immunity of porkling；
（3）It is of the invention prepared and add antibacterial peptide, have that molecular weight is small, thermal stability is good, non-immunogenicity, has a broad antifungal spectrum,
Can it is antimycotic, viral and reduce parasite activity, be not likely to produce drug resistance, becoming can be with a kind of green of substitute antibiotics
Type additive.
Specific embodiment
Antibacterial peptide producing strains：Lactobacillus plantarum, Bifidobacterium in mass ratio 3:2 mixing.
Antibacterial peptide seed broth bouillon：According to the mass fraction, 8 ~ 10 parts of peptone, 1 ~ 2 part of beef extract, NaCl 4 ~ 6 are taken
Part, 7.2 ± 0.2,121 DEG C of high pressure steam sterilization 20min of pH.
Antibacterial peptide fermentation culture：According to the mass fraction, 10 ~ 12 parts of sucrose, 18 ~ 20 parts of corn flour, CaCl are taken23 ~ 5 parts,
ZnCl212 ~ 15 parts, 0.1 ~ 0.6 part of Tween-20,1000 parts, pH7.2 ± 0.2,121 DEG C high pressure steam sterilization 20min of water.
Pannage fermentation raw material：Fresh grapefruit skin slag, wheat bran, wheat flour, chaff of uniting, in mass ratio 7:3:2:4 are uniformly mixed.
Fermenting raw materials strain：Lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing.
The formula of strain activation and culture base is：According to the mass fraction, 16 ~ 19 parts of yeast extract, 10 ~ 12 parts of peptone, Portugal are taken
9 ~ 12 parts of grape sugar, CaCO31.7g, 1000 parts of water, 121 DEG C of sterilizing 30min.
The configuration of feed fermentation nutrient solution：It counts in parts by weight, takes 12 ~ 15 parts of corn flour, 20 ~ 24 parts of dregs of beans, beef extract 8
~ 10 parts, 6 ~ 9 parts of peptone, 13 ~ 17 parts of glucose, 18 ~ 24 parts of wheat bran, MgSO4·7H20.2 ~ 0.5 part of O, FeSO4·7H2O
0.02 ~ 0.03 part, CaCO33 ~ 5 parts, 1000 parts of water, stir evenly.
A kind of preparation of antibacterial peptide pannage, includes the following steps：
1. take antibacterial peptide producing strains in mass ratio 1:9 are inoculated into antibacterial peptide seed broth bouillon, and 33 ~ 35 DEG C are cultivated 2 ~ 3 days, are obtained
Antibacterial peptide zymophyte suspension, by antibacterial peptide fermenting microbe bacteria suspension and fermentation culture in mass ratio 2:5 add in fermentation tank, fermentation
Throughput is set as 0.4 ~ 2.0m3/ h, rotating speed 400r/min, dissolved oxygen are 50 ~ 70%, 32 ~ 35 DEG C of fermentation temperature, fermentation time
14 ~ 18h obtains antibacterial peptide zymotic fluid, and antibacterial peptide fermentate is placed in 45 ~ 50 DEG C of constant temperature blender with magnetic force, adds in wheat bran, wheat bran
Mass ratio with antibacterial peptide fermentate is 2:7,0.5 ~ 1h is adsorbed, antibacterial peptide zymotic fluid and wheat bran are subjected to screening point after finishing
From, zymotic fluid is removed, collects wheat bran, it is dry to constant weight, obtain dry antibacterial peptide-wheat bran compound；
2. by pannage raw material and water in mass ratio 5:4 fully moisturize mixing, 121 DEG C of sterilizing 30min, and raw material after must sterilizing is cold
But to room temperature, under aseptic condition, mannonase pectase, cellulase, mannonase pectase, cellulase are added in
Mass ratio with raw material after sterilizing is 5:4:2:7,60 ~ 72h of constant temperature is digested under 45 ~ 50 DEG C of temperature conditionss, rocks 2 daily
It is secondary, feedstuff after must digesting；
3. by lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing, obtains fermenting microbe mixture,
Fermenting microbe mixture is seeded in strain activation and culture base, 23 ~ 30 DEG C are cultivated 3 ~ 5 days, obtain fermenting microbe suspension, mistake
Filter, removes mycelium, obtains fermenting microbe seed liquor；Feedstuff after above-mentioned dry antibacterial peptide-wheat bran compound, enzymolysis is added in and is raised
Expect in fermented nutritive liquid, feedstuff, the mass ratio of feed fermentation nutrient solution are after dry antibacterial peptide-wheat bran compound, enzymolysis
3:5:12, mixed fodder fermentate is obtained, fermenting microbe seed liquor is seeded to mixed fodder hair by the inoculum concentration of mass fraction 10%
In ferment object, under 30 ~ 40 DEG C of temperature conditionss, ferment 72 ~ 96h, obtains antibacterial peptide pannage fermentate, and antibacterial peptide pannage is taken to ferment
Object is dried, and obtains antibacterial peptide pannage.
Example 1
Antibacterial peptide producing strains：Lactobacillus plantarum, Bifidobacterium in mass ratio 3:2 mixing.
Antibacterial peptide seed broth bouillon：According to the mass fraction, 8 parts of peptone, 1 part of beef extract, 4 parts, pH 7 of NaCl,
121 DEG C of high pressure steam sterilization 20min.
Antibacterial peptide fermentation culture：According to the mass fraction, 10 parts of sucrose, 18 parts of corn flour, CaCl are taken23 parts, ZnCl212 parts,
0.1 part of Tween-20,1000 parts, pH7,121 DEG C high pressure steam sterilization 20min of water.
Pannage fermentation raw material：Fresh grapefruit skin slag, wheat bran, wheat flour, chaff of uniting, in mass ratio 7:3:2:4 are uniformly mixed.
Fermenting raw materials strain：Lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing.
The formula of strain activation and culture base is：According to the mass fraction, 16 parts of yeast extract, 10 parts of peptone, glucose 9 are taken
Part, CaCO31.7g, 1000 parts of water, 121 DEG C of sterilizing 30min.
The configuration of feed fermentation nutrient solution：It counts in parts by weight, takes 12 parts of corn flour, 20 parts of dregs of beans, 8 parts of beef extract, egg
White 6 parts of peptone, 13 parts of glucose, 18 parts of wheat bran, MgSO4·7H20.2 part of O, FeSO4·7H20.02 part of O, CaCO33 parts, water
It 1000 parts, stirs evenly.
A kind of preparation of antibacterial peptide pannage, includes the following steps：
1. take antibacterial peptide producing strains in mass ratio 1:9 are inoculated into antibacterial peptide seed broth bouillon, and 33 DEG C are cultivated 2 days, obtain antibacterial
Peptide fermenting microbe bacteria suspension, by antibacterial peptide fermenting microbe bacteria suspension and fermentation culture in mass ratio 2:5 add in fermentation tank, fermentation
Throughput is set as 0.4m3/ h, rotating speed 400r/min, dissolved oxygen 50%, 32 DEG C of fermentation temperature, fermentation time 14h obtain antibacterial
Antibacterial peptide fermentate is placed in 45 DEG C of constant temperature blender with magnetic force by peptide zymotic fluid, adds in wheat bran, wheat bran and antibacterial peptide fermentate
Mass ratio is 2:7,0.5h is adsorbed, with wheat bran separate by antibacterial peptide zymotic fluid after finishing, removes zymotic fluid, collects bran
Skin, it is dry to constant weight, obtain dry antibacterial peptide-wheat bran compound；
2. by pannage raw material and water in mass ratio 5:4 fully moisturize mixing, 121 DEG C of sterilizing 30min, and raw material after must sterilizing is cold
But to room temperature, under aseptic condition, mannonase pectase, cellulase, mannonase pectase, cellulase are added in
Mass ratio with raw material after sterilizing is 5:4:2:7, enzymolysis mixture is obtained, mixture constant temperature under 45 DEG C of temperature conditionss will be digested
60h is digested, and is rocked daily 2 times, feedstuff after must digesting；
3. by lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing, obtains fermenting microbe mixture,
Fermenting microbe mixture is seeded in strain activation and culture base, 23 DEG C are cultivated 3 days, obtain fermenting microbe suspension, are filtered, are gone bacterium
Filament obtains fermenting microbe seed liquor；Feedstuff after above-mentioned dry antibacterial peptide-wheat bran compound, enzymolysis is added in into feed fermentation
In nutrient solution, feedstuff, the mass ratio of feed fermentation nutrient solution are 3 after dry antibacterial peptide-wheat bran compound, enzymolysis:5:12,
Mixed fodder fermentate is obtained, fermenting microbe seed liquor is seeded in mixed fodder fermentate by the inoculum concentration of mass fraction 10%,
Under 30 ~ 40 DEG C of temperature conditionss, ferment 72h, obtains antibacterial peptide pannage fermentate, and antibacterial peptide pannage fermentate is taken to dry, is obtained
Antibacterial peptide pannage.
Example 2
Antibacterial peptide producing strains：Lactobacillus plantarum, Bifidobacterium in mass ratio 3:2 mixing.
Antibacterial peptide seed broth bouillon：According to the mass fraction, 10 parts of peptone, 2 parts of beef extract, 6 parts of NaCl, pH
7.4,121 DEG C of high pressure steam sterilization 20min.
Antibacterial peptide fermentation culture：According to the mass fraction, 12 parts of sucrose, 20 parts of corn flour, CaCl are taken25 parts, ZnCl215 parts,
0.6 part of Tween-20,1000 parts, pH7.4,121 DEG C high pressure steam sterilization 20min of water.
Pannage fermentation raw material：Fresh grapefruit skin slag, wheat bran, wheat flour, chaff of uniting, in mass ratio 7:3:2:4 are uniformly mixed.
Fermenting raw materials strain：Lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing.
The formula of strain activation and culture base is：According to the mass fraction, 19 parts of yeast extract, 12 parts of peptone, glucose 12 are taken
Part, CaCO31.7g, 1000 parts of water, 121 DEG C of sterilizing 30min.
The configuration of feed fermentation nutrient solution：It counts in parts by weight, takes 15 parts of corn flour, 24 parts of dregs of beans, 10 parts of beef extract, egg
White 9 parts of peptone, 17 parts of glucose, 24 parts of wheat bran, MgSO4·7H2O0.5 parts, FeSO4·7H20.03 part of O, CaCO35 parts, water 1000
Part, it stirs evenly.
A kind of preparation of antibacterial peptide pannage, includes the following steps：
1. take antibacterial peptide producing strains in mass ratio 1:9 are inoculated into antibacterial peptide seed broth bouillon, and 35 DEG C are cultivated 3 days, obtain antibacterial
Peptide fermenting microbe bacteria suspension, by antibacterial peptide fermenting microbe bacteria suspension and fermentation culture in mass ratio 2:5 add in fermentation tank, fermentation
Throughput is set as 2.0m3/ h, rotating speed 400r/min, dissolved oxygen 70%, 35 DEG C of fermentation temperature, fermentation time 18h obtain antibacterial
Antibacterial peptide fermentate is placed in 50 DEG C of constant temperature blender with magnetic force by peptide zymotic fluid, adds in wheat bran, wheat bran and antibacterial peptide fermentate
Mass ratio is 2:7,1h is adsorbed, with wheat bran separate by antibacterial peptide zymotic fluid after finishing, removes zymotic fluid, collects bran
Skin, it is dry to constant weight, obtain dry antibacterial peptide-wheat bran compound；
2. by pannage raw material and water in mass ratio 5:4 fully moisturize mixing, 121 DEG C of sterilizing 30min, and raw material after must sterilizing is cold
But to room temperature, under aseptic condition, mannonase pectase, cellulase, mannonase pectase, cellulase are added in
Mass ratio with raw material after sterilizing is 5:4:2:7, enzymolysis mixture is obtained, mixture constant temperature under 50 DEG C of temperature conditionss will be digested
72h is digested, and is rocked daily 2 times, feedstuff after must digesting；
3. by lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing, obtains fermenting microbe mixture,
Fermenting microbe mixture is seeded in strain activation and culture base, 30 DEG C are cultivated 5 days, obtain fermenting microbe suspension, are filtered, are gone bacterium
Filament obtains fermenting microbe seed liquor；Feedstuff after above-mentioned dry antibacterial peptide-wheat bran compound, enzymolysis is added in into feed fermentation
In nutrient solution, feedstuff, the mass ratio of feed fermentation nutrient solution are 3 after dry antibacterial peptide-wheat bran compound, enzymolysis:5:12,
Mixed fodder fermentate is obtained, fermenting microbe seed liquor is seeded in mixed fodder fermentate by the inoculum concentration of mass fraction 10%,
Under 40 DEG C of temperature conditionss, ferment 96h, obtains antibacterial peptide pannage fermentate, and antibacterial peptide pannage fermentate is taken to dry, obtains antibacterial
Peptide pannage.
Example 3
Antibacterial peptide producing strains：Lactobacillus plantarum, Bifidobacterium in mass ratio 3:2 mixing.
Antibacterial peptide seed broth bouillon：According to the mass fraction, 9 parts of peptone, 1.5 parts of beef extract, 5 parts of NaCl, pH
7.2,121 DEG C of high pressure steam sterilization 20min.
Antibacterial peptide fermentation culture：According to the mass fraction, 11 parts of sucrose, 19 parts of corn flour, CaCl are taken24 parts, ZnCl213 parts,
0.4 part of Tween-20,1000 parts, pH7.2,121 DEG C high pressure steam sterilization 20min of water.
Pannage fermentation raw material：Fresh grapefruit skin slag, wheat bran, wheat flour, chaff of uniting, in mass ratio 7:3:2:4 are uniformly mixed.
Fermenting raw materials strain：Lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing.
The formula of strain activation and culture base is：According to the mass fraction, 17 parts of yeast extract, 11 parts of peptone, glucose 10 are taken
Part, CaCO31.7g, 1000 parts of water, 121 DEG C of sterilizing 30min.
The configuration of feed fermentation nutrient solution：It counts in parts by weight, takes 13 parts of corn flour, 22 parts of dregs of beans, 9 parts of beef extract, egg
White 7 parts of peptone, 15 parts of glucose, 21 parts of wheat bran, MgSO4·7H20.4 part of O, FeSO4·7H20.02 part of O, CaCO34 parts, water
It 1000 parts, stirs evenly.
A kind of preparation of antibacterial peptide pannage, includes the following steps：
1. take antibacterial peptide producing strains in mass ratio 1:9 are inoculated into antibacterial peptide seed broth bouillon, and 34 DEG C are cultivated 2.5 days, are obtained anti-
Bacterium peptide fermenting microbe bacteria suspension, by antibacterial peptide fermenting microbe bacteria suspension and fermentation culture in mass ratio 2:5 add in fermentation tank, hair
Ferment throughput is set as 1.2m3/ h, rotating speed 400r/min, dissolved oxygen 60%, 34 DEG C of fermentation temperature, fermentation time 16h are obtained anti-
Antibacterial peptide fermentate is placed in 47 DEG C of constant temperature blender with magnetic force by bacterium peptide zymotic fluid, adds in wheat bran, wheat bran and antibacterial peptide fermentate
Mass ratio be 2:7,0.7h is adsorbed, with wheat bran separate by antibacterial peptide zymotic fluid after finishing, removes zymotic fluid, is collected
Wheat bran, it is dry to constant weight, obtain dry antibacterial peptide-wheat bran compound；
2. by pannage raw material and water in mass ratio 5:4 fully moisturize mixing, 121 DEG C of sterilizing 30min, and raw material after must sterilizing is cold
But to room temperature, under aseptic condition, mannonase pectase, cellulase, mannonase pectase, cellulase are added in
Mass ratio with raw material after sterilizing is 5:4:2:7, enzymolysis mixture is obtained, mixture constant temperature under 47 DEG C of temperature conditionss will be digested
66h is digested, and is rocked daily 2 times, feedstuff after must digesting；
3. by lactic acid bacteria, bacillus subtilis, candida utili bacterium in mass ratio 2:1:1 mixing, obtains fermenting microbe mixture,
Fermenting microbe mixture is seeded in strain activation and culture base, 26 DEG C are cultivated 4 days, obtain fermenting microbe suspension, are filtered, are gone bacterium
Filament obtains fermenting microbe seed liquor；Feedstuff after above-mentioned dry antibacterial peptide-wheat bran compound, enzymolysis is added in into feed fermentation
In nutrient solution, feedstuff, the mass ratio of feed fermentation nutrient solution are 3 after dry antibacterial peptide-wheat bran compound, enzymolysis:5:12,
Mixed fodder fermentate is obtained, fermenting microbe seed liquor is seeded in mixed fodder fermentate by the inoculum concentration of mass fraction 10%,
Under 35 DEG C of temperature conditionss, ferment 84h, obtains antibacterial peptide pannage fermentate, and antibacterial peptide pannage fermentate is taken to dry, obtains antibacterial
Peptide pannage.
Reference examples：The pig fermentative feedstuff of microbe of Yichun City company production.
Pig obtained by examples detailed above is subjected to nutritional ingredient with fermentative feedstuff of microbe and the fermentative feedstuff of microbe of reference examples
Detection.The feed and the feed of reference examples prepared using above-described embodiment carries out pork pig feeding comparative trial.Selective body heavy phase is near,
The piggy of 40 ages in days 200, is randomly divided into control group, experimental group, carries out 100 daily gain, feedstuff-meat ratio and death by a definite date in total
The contrast tests such as rate, experimental period, use conventional barrier, and every morning, each feeding in afternoon once, ensure that all pigs can freely be adopted
Food.In feeding process, 6 points of every morning weighs empty stomach pig, records data.By the experiment of 100 days by a definite date, as a result unite
Meter such as table 1：
Table 1：
Summary, fermentative feedstuff of microbe of the invention are more preferable to swine rearing effect, are worth of widely use.
Claims (8)
1. a kind of preparation method of antibacterial peptide pannage, which is characterized in that the preparation method includes the following steps：
（1）Lactobacillus plantarum and Bifidobacteria powder is taken to mix, obtains mixing bacterium powder, mixing bacterium powder is seeded to antibacterial peptide seed meat
Soup culture medium, culture, obtains antibacterial peptide zymophyte suspension, and antibacterial peptide fermenting microbe bacteria suspension and fermentation culture are added in and fermented
Tank obtains antibacterial peptide zymotic fluid, and antibacterial peptide fermentate is placed in constant temperature blender with magnetic force, adds in wheat bran, is adsorbed, after finishing
Antibacterial peptide zymotic fluid with wheat bran separate, removes zymotic fluid, collects wheat bran, it is dry to constant weight, obtain dry antibacterial peptide-
Wheat bran compound；
（2）Water is added to mix pannage raw material and fully moisturize mixing, 121 DEG C of sterilizing 30min, raw material after must sterilizing is cooled to room
Temperature adds in mannonase pectase, cellulase, is digested, rocked daily 2 times, feedstuff after must digesting；
（3）Lactic acid bacteria, bacillus subtilis, candida utili bacterium are mixed, fermentation mixed bacteria is obtained, fermenting microbe is mixed
Object is seeded in strain activation and culture base and cultivates, and obtains fermenting microbe suspension, and filtering removes mycelium, takes filtrate, obtains fermenting microbe
Feedstuff after above-mentioned dry antibacterial peptide-wheat bran compound, enzymolysis is added in feed fermentation nutrient solution, must mix feeding by seed
Expect fermentate, fermenting microbe seed liquor is seeded in mixed fodder fermentate by the inoculum concentration of mass fraction 10%, ferment, obtain
Antibacterial peptide pannage fermentate takes antibacterial peptide pannage fermentate to dry, obtains antibacterial peptide pannage.
2. the preparation method of antibacterial peptide pannage according to claim 1, which is characterized in that the step（1）Middle antibacterial
Peptide producing strains are lactobacillus plantarum and Bifidobacterium in mass ratio 3:2 mixing, the formula of antibacterial peptide seed broth bouillon be by
Mass fraction meter, 8 ~ 10 parts of peptone, 1 ~ 2 part of beef extract, 7.2 ± 0.2,121 DEG C of high pressure steam sterilizations of 4 ~ 6 parts of NaCl, pH
20min；The formula of antibacterial peptide fermentation culture is according to the mass fraction, to take 10 ~ 12 parts of sucrose, 18 ~ 20 parts of corn flour, CaCl2
3 ~ 5 parts, ZnCl212 ~ 15 parts, 0.1 ~ 0.6 part of Tween-20,1000 parts, pH7.2 ± 0.2,121 DEG C high pressure steam sterilizations of water
20min。
3. the preparation method of antibacterial peptide pannage according to claim 1, which is characterized in that the step（1）Middle antibacterial
The mass ratio of peptide producing strains and antibacterial peptide seed broth bouillon is 1:9, antibacterial peptide fermenting microbe bacteria suspension and fermentation culture
Mass ratio be 2:5, fermentation condition is：Fermentation throughput is set as 0.4 ~ 2.0m3/ h, rotating speed 400r/min, dissolved oxygen be 50 ~
70%th, 32 ~ 35 DEG C of fermentation temperature, 14 ~ 18h of fermentation time.
4. the preparation method of antibacterial peptide pannage according to claim 1, which is characterized in that the step（1）Middle wheat bran
Mass ratio with antibacterial peptide fermentate is 2:7, adsorb 0.5 ~ 1h.
5. the preparation method of antibacterial peptide pannage according to claim 1, which is characterized in that the step（2）Middle pig is raised
Expect fermentation raw material include fresh grapefruit skin slag, wheat bran, wheat flour, unite chaff, in mass ratio 7:3:2:4 are uniformly mixed.
6. the preparation method of antibacterial peptide pannage according to claim 1, which is characterized in that the step（2）Middle feed
The mass ratio of fermentation raw material and water is 5:4, the mass ratio of raw material is 5 after mannonase pectase, cellulase and sterilizing:
4:2:7, the condition of enzymolysis is 40 ~ 45 DEG C of 60 ~ 72h of constant temperature.
7. the preparation method of antibacterial peptide pannage according to claim 1, which is characterized in that the step（3）Middle lactic acid
Bacterium, bacillus subtilis, the mass ratio of candida utili bacterium are 2:1:1, dry antibacterial peptide-wheat bran compound is raised after digesting
Expect raw material, the mass ratio of feed fermentation nutrient solution is 3:5:12.
8. the preparation method of antibacterial peptide pannage according to claim 1, which is characterized in that the step（3）Middle strain
The formula of activation medium is：According to the mass fraction, 16 ~ 19 parts of yeast extract is taken, 10 ~ 12 parts of peptone, 9 ~ 12 parts of glucose,
CaCO31.7 ~ 2.2 parts, 1000 parts of water, 121 DEG C of sterilizing 30min；The configuration of feed fermentation nutrient solution：It counts, takes in parts by weight
12 ~ 15 parts of corn flour, 20 ~ 24 parts of dregs of beans, 8 ~ 10 parts of beef extract, 6 ~ 9 parts of peptone, 13 ~ 17 parts of glucose, 18 ~ 24 parts of wheat bran,
MgSO4·7H20.2 ~ 0.5 part of O, FeSO4·7H20.02 ~ 0.03 part of O, CaCO33 ~ 5 parts, 1000 parts of water, stir evenly.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111990530A (en) * | 2020-09-07 | 2020-11-27 | 广东海纳川生物科技股份有限公司 | Preparation method of feed protein raw material |
| CN112913964A (en) * | 2021-02-06 | 2021-06-08 | 广安鑫农发展有限公司 | Fermented feed and preparation method thereof |
| CN114009615A (en) * | 2021-11-25 | 2022-02-08 | 合肥盛嘉生物科技有限公司 | Disease-resistant sow feed and preparation method thereof |
| CN114391604A (en) * | 2022-01-04 | 2022-04-26 | 清远一生自然生物研究院有限公司 | Method for preparing feed additive containing antibacterial peptide |
-
2017
- 2017-12-12 CN CN201711315434.0A patent/CN108077617A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111990530A (en) * | 2020-09-07 | 2020-11-27 | 广东海纳川生物科技股份有限公司 | Preparation method of feed protein raw material |
| CN112913964A (en) * | 2021-02-06 | 2021-06-08 | 广安鑫农发展有限公司 | Fermented feed and preparation method thereof |
| CN114009615A (en) * | 2021-11-25 | 2022-02-08 | 合肥盛嘉生物科技有限公司 | Disease-resistant sow feed and preparation method thereof |
| CN114391604A (en) * | 2022-01-04 | 2022-04-26 | 清远一生自然生物研究院有限公司 | Method for preparing feed additive containing antibacterial peptide |
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Application publication date: 20180529 |