CN108030096A - A kind of calcium iron zinc and probiotic composition and its application - Google Patents
A kind of calcium iron zinc and probiotic composition and its application Download PDFInfo
- Publication number
- CN108030096A CN108030096A CN201711365163.XA CN201711365163A CN108030096A CN 108030096 A CN108030096 A CN 108030096A CN 201711365163 A CN201711365163 A CN 201711365163A CN 108030096 A CN108030096 A CN 108030096A
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- CN
- China
- Prior art keywords
- calcium
- zinc
- iron
- source
- probiotics
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 66
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 66
- 239000000203 mixture Substances 0.000 title claims abstract description 45
- JYMOQWNEIZBVFC-UHFFFAOYSA-N [Fe].[Zn].[Ca] Chemical compound [Fe].[Zn].[Ca] JYMOQWNEIZBVFC-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 27
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 116
- 239000011701 zinc Substances 0.000 claims abstract description 71
- 239000011575 calcium Substances 0.000 claims abstract description 65
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 58
- 229910052725 zinc Inorganic materials 0.000 claims abstract description 57
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 54
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 54
- 229910052742 iron Inorganic materials 0.000 claims abstract description 53
- 239000004375 Dextrin Substances 0.000 claims abstract description 20
- 229920001353 Dextrin Polymers 0.000 claims abstract description 20
- 235000019425 dextrin Nutrition 0.000 claims abstract description 20
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 claims abstract description 15
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 229960005069 calcium Drugs 0.000 claims description 52
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 26
- 241001608472 Bifidobacterium longum Species 0.000 claims description 23
- 229940009291 bifidobacterium longum Drugs 0.000 claims description 23
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 19
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 19
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 19
- 239000004222 ferrous gluconate Substances 0.000 claims description 14
- 235000013924 ferrous gluconate Nutrition 0.000 claims description 14
- 229960001645 ferrous gluconate Drugs 0.000 claims description 14
- VRIVJOXICYMTAG-IYEMJOQQSA-L iron(ii) gluconate Chemical compound [Fe+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O VRIVJOXICYMTAG-IYEMJOQQSA-L 0.000 claims description 14
- 241001465754 Metazoa Species 0.000 claims description 13
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 13
- 229960003563 calcium carbonate Drugs 0.000 claims description 13
- 235000010216 calcium carbonate Nutrition 0.000 claims description 13
- 241000186000 Bifidobacterium Species 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 239000004227 calcium gluconate Substances 0.000 claims description 8
- 235000013927 calcium gluconate Nutrition 0.000 claims description 8
- 229960004494 calcium gluconate Drugs 0.000 claims description 8
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 claims description 8
- 239000011706 ferric diphosphate Substances 0.000 claims description 8
- 235000007144 ferric diphosphate Nutrition 0.000 claims description 8
- CADNYOZXMIKYPR-UHFFFAOYSA-B ferric pyrophosphate Chemical compound [Fe+3].[Fe+3].[Fe+3].[Fe+3].[O-]P([O-])(=O)OP([O-])([O-])=O.[O-]P([O-])(=O)OP([O-])([O-])=O.[O-]P([O-])(=O)OP([O-])([O-])=O CADNYOZXMIKYPR-UHFFFAOYSA-B 0.000 claims description 8
- 229940036404 ferric pyrophosphate Drugs 0.000 claims description 8
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 7
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical group [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 5
- 229960001763 zinc sulfate Drugs 0.000 claims description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 5
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 4
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 4
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 235000013373 food additive Nutrition 0.000 claims description 4
- 239000002778 food additive Substances 0.000 claims description 4
- WGIWBXUNRXCYRA-UHFFFAOYSA-H trizinc;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [Zn+2].[Zn+2].[Zn+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O WGIWBXUNRXCYRA-UHFFFAOYSA-H 0.000 claims description 4
- 239000011746 zinc citrate Substances 0.000 claims description 4
- 235000006076 zinc citrate Nutrition 0.000 claims description 4
- 229940068475 zinc citrate Drugs 0.000 claims description 4
- WHMDKBIGKVEYHS-IYEMJOQQSA-L Zinc gluconate Chemical compound [Zn+2].OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O WHMDKBIGKVEYHS-IYEMJOQQSA-L 0.000 claims description 3
- 239000011670 zinc gluconate Substances 0.000 claims description 3
- 235000011478 zinc gluconate Nutrition 0.000 claims description 3
- 229960000306 zinc gluconate Drugs 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 239000001736 Calcium glycerylphosphate Substances 0.000 claims description 2
- DKKCQDROTDCQOR-UHFFFAOYSA-L Ferrous lactate Chemical compound [Fe+2].CC(O)C([O-])=O.CC(O)C([O-])=O DKKCQDROTDCQOR-UHFFFAOYSA-L 0.000 claims description 2
- CANRESZKMUPMAE-UHFFFAOYSA-L Zinc lactate Chemical compound [Zn+2].CC(O)C([O-])=O.CC(O)C([O-])=O CANRESZKMUPMAE-UHFFFAOYSA-L 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- MDXRFOWKIZPNTA-UHFFFAOYSA-L butanedioate;iron(2+) Chemical compound [Fe+2].[O-]C(=O)CCC([O-])=O MDXRFOWKIZPNTA-UHFFFAOYSA-L 0.000 claims description 2
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 claims description 2
- 239000001639 calcium acetate Substances 0.000 claims description 2
- 229960005147 calcium acetate Drugs 0.000 claims description 2
- 235000011092 calcium acetate Nutrition 0.000 claims description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 229960002713 calcium chloride Drugs 0.000 claims description 2
- 235000011148 calcium chloride Nutrition 0.000 claims description 2
- FNAQSUUGMSOBHW-UHFFFAOYSA-H calcium citrate Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FNAQSUUGMSOBHW-UHFFFAOYSA-H 0.000 claims description 2
- 239000001354 calcium citrate Substances 0.000 claims description 2
- 229960004256 calcium citrate Drugs 0.000 claims description 2
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 2
- 229940095618 calcium glycerophosphate Drugs 0.000 claims description 2
- UHHRFSOMMCWGSO-UHFFFAOYSA-L calcium glycerophosphate Chemical compound [Ca+2].OCC(CO)OP([O-])([O-])=O UHHRFSOMMCWGSO-UHFFFAOYSA-L 0.000 claims description 2
- 235000019299 calcium glycerylphosphate Nutrition 0.000 claims description 2
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 2
- 239000001527 calcium lactate Substances 0.000 claims description 2
- 235000011086 calcium lactate Nutrition 0.000 claims description 2
- 229960002401 calcium lactate Drugs 0.000 claims description 2
- 239000001506 calcium phosphate Substances 0.000 claims description 2
- 229960001714 calcium phosphate Drugs 0.000 claims description 2
- 235000011010 calcium phosphates Nutrition 0.000 claims description 2
- 229940095672 calcium sulfate Drugs 0.000 claims description 2
- 235000011132 calcium sulphate Nutrition 0.000 claims description 2
- MKJXYGKVIBWPFZ-CEOVSRFSSA-L calcium;(2s)-2-hydroxypropanoate Chemical compound [Ca+2].C[C@H](O)C([O-])=O.C[C@H](O)C([O-])=O MKJXYGKVIBWPFZ-CEOVSRFSSA-L 0.000 claims description 2
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- 239000004225 ferrous lactate Substances 0.000 claims description 2
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- 229960001604 ferrous succinate Drugs 0.000 claims description 2
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- 239000011790 ferrous sulphate Substances 0.000 claims description 2
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 2
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- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 3
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims 1
- 241000194017 Streptococcus Species 0.000 claims 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2400/515—Animalis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
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Abstract
The invention discloses a kind of calcium iron zinc and probiotic composition, the composition is made of the raw material of following weight proportion：1~10 part of calcium source, 0.1~0.5 part of source of iron, 0.1~0.5 part of zinc source, 0.1~2 part of probiotics, 1~10 part of stachyose, 1~10 part of oligofructose, 1~10 part of resistant dextrin.Overcome the deficiencies in the prior art of the present invention, selects the probiotics strain for having the effect that remarkably promotes to calcium iron zinc-iron alloy solution, and exploitation is a kind of new by calcium iron zinc and probiotic composition.Zoopery shows, promotion absorption significant effect of the said composition to calcium iron zinc, compared with the same type composition using plain edition probiotics strain, the absorptivity of said composition calcium iron zinc is obviously improved, calcium absorptivity improves 58.71%, iron absorptivity improves 60.70%, and zinc absorption improves 44.30%.
Description
(1) technical field
The present invention relates to a kind of calcium, iron, zinc and probiotic composition and its application in functional food field, said composition
It is primarily to facilitate absorption of the body to calcium, iron, zinc.
(2) background technology
Calcium, iron, zinc are 3 kinds of mineral matters needed by human, and important physiology work(is played during upgrowth and development of children
Can, if in 3 kinds of upgrowth and development of children stage calcium, iron, zinc mineral matter insufficiency of intakes, corresponding disease can be triggered to even result in
Virgin growth retardation.
Calcium is the most mineral matter of body burden, its main Physiological Function is to form bone and tooth, and human body is daily from food
The mineral matters such as the calcium that is obtained in thing, phosphorus are deposited in bone tissue to ensure the normal growth of bone.Iron is the important of human body hematopoiesis
Raw material, is the nutrient for maintaining normal human's vital movement indispensable.The common disease of asiderosis is hypoferric anemia.People
The required nutrients of many tissues of body are transported by haemocyte, and human body has lacked iron, and it is low to will result in hemoglobin,
The conveying of oxygen is influenced, so that the growth of children is affected.Research shows that children's iron deficiency can cause hypoferric anemia,
It can influence the intellectual development of children.Zinc is one of constituent of gustin, and zinc deficiency can make taste function decline, and easily lead to viscous
Film hyperplasia and parakeratosis, make oral mucosa be easy to come off, and the epithelial cell to come off blocks nipple taste bud aperture, Gustatory sensitivity
Reduce, saliva alkaline phosphatase is reduced, taste bud hypofunction, causes children's anorexia, apocleisis, diarrhea, canker sore, different
Eat addiction, growth retardation；Zinc deficiency can also result in sallow alopecia, hair, pachylosis easy infection, nervous system development by
Resistance, feeblemindedness.Early stage carries out the prevention and intervention of zinc-deficiency, avoids the occurrence of " allotriophagy ", growth and development and intelligence to children
Development has facilitation.
The maintenance of calcium, iron, zinc to human normal physiological function, the particularly body of children and intellectual development have weight
Act on, so should be replenished in time.But supplement is that on the one hand, on the other hand absorption is.Calcium, iron, zinc how is promoted to have
Effect absorbs the fundamental way for being only and solving the problems, such as.There is absorption of the studies have shown that probiotics to mineral element that there is positive promotion to make
With as bifidobacterium lactis, streptococcus thermophilus have facilitation to calcium, magnesium absorption；Lactobacillus plantarum, bifidobacterium longum etc. pair
The absorption of iron, zinc has facilitation.It is but different to the facilitation of mineral element absorption between different strains.
At present, in the market has been known as the Related product and patent of full nutrition, and also profitable probliotics are taken in some of which formula
Match somebody with somebody.These formulas are the concept for emphasizing full nutrition mostly, and probiotics is played the role of being only intestinal microecology wherein
Adjust, that is, absorption of the strain added to calcium iron zinc not targetedly facilitation.In addition, be have it is some declare it is prebiotic
Bacterium promotes the formula patent or product of calcium iron zinc-iron alloy solution, its strain and bacterial strain for selecting, because not accounting for strain specificity
Otherness in terms of to promoting calcium iron zinc-iron alloy solution, so effect is unsatisfactory.Consideration based on problem above, selects to calcium iron zinc
The probiotics with specific aim facilitation is absorbed, a kind of composition for effectively facilitating calcium iron zinc-iron alloy solution is developed based on this and matches somebody with somebody
Side, can make up the deficiency of existing product/formula.
(3) content of the invention
The present invention seeks to overcome the shortcomings of the said goods and technology, screen has specific aim facilitation to calcium iron zinc
Probiotics strain, develops a kind of new calcium iron zinc and probiotic composition, really realizes that the effectively same of calcium iron zinc is mended.
The technical solution adopted by the present invention is：
The present invention provides a kind of calcium iron zinc and probiotic composition, the composition by following weight proportion raw material group
Into：It is 1~10 part of calcium source, 0.1~0.5 part of source of iron, 0.1~0.5 part of zinc source, 0.1~2 part of probiotics, 1~10 part of stachyose, low
1~10 part of Fructooligosaccharides, 1~10 part of resistant dextrin；(viable count is 10 to the probiotics10CFU/g) it is lactobacillus plantarum
(Lactobacillus plantarum), bifidobacterium longum (Bifidobacterium longum), streptococcus thermophilus
One kind in (Streptococcus thermophilus) or animal bifidobacteria (Bifidobacterium animalis)
Or the combination of several arbitrary proportions；The calcium source dosage is used with calcareous gauge, the source of iron dosage with irony gauge, the zinc source
Amount is in terms of zinc quality.
Further, the calcium source is calcium carbonate, calcium acetate, calcium chloride, calcium gluconate, calcium citrate, calcium lactate, phosphoric acid
The group of one or more of arbitrary proportions in hydrogen calcium, calcium dihydrogen phosphate, calcium phosphate, calcium sulfate, L-calcium lactate or calcium glycerophosphate
Close, more preferably calcium carbonate or calcium gluconate.
Further, the source of iron for ferrous gluconate, ferrous fumarate, ferric pyrophosphate, ferrous sulfate, ferrous lactate or
The combination of one or more of arbitrary proportions in ferrous succinate, more preferably ferrous gluconate or ferric pyrophosphate.
Further, the zinc source be zinc sulfate, zinc citrate, zinc gluconate, zinc oxide or zinc lactate in one kind or
The combination of several arbitrary proportions, more preferably zinc sulfate or zinc gluconate.
Further, the probiotics is preferably lactobacillus plantarum 299v, bifidobacterium longum BL88-Only, streptococcus thermophilus
The mixing of one or more arbitrary proportions in ST-6 or animal bifidobacteria B-94.
Further, the probiotics is one of following：(1) lactobacillus plantarum 299v and bifidobacterium longum BL88-Only
With mass ratio 1:1 mixing；(2) lactobacillus plantarum 299v and animal bifidobacteria B-94 is with mass ratio 1:1 mixing；(3) plant
Thing lactobacillus 299v and streptococcus thermophilus ST-6 is with mass ratio 1:1 mixing；(4) animal bifidobacteria B-94 and bifidobacterium longum
BL88-Only is with mass ratio 1:1 mixing.
Further, the calcium iron zinc is made of with probiotic composition the raw material of following weight proportion：10 parts of calcium source, source of iron
0.5 part, 0.5 part of zinc source, 2 parts of probiotics, 10 parts of stachyose, 10 parts of oligofructose, 10 parts of resistant dextrin；The calcium source is carbonic acid
Calcium or calcium gluconate；The source of iron is ferrous gluconate or ferric pyrophosphate；The zinc source is zinc sulfate or zinc citrate；Institute
It is lactobacillus plantarum 299v and bifidobacterium longum BL88-Only with mass ratio 1 to state probiotics:1 mixing.
It is described the present invention also provides a kind of calcium iron zinc and application of the probiotic composition in food additives are prepared
Food additives are pulvis, granule, tablet or capsule.
Calcium iron zinc of the present invention is with probiotic composition preparation method：By resistant dextrin, calcium source, source of iron, zinc source, water
Threose, oligofructose cross 40 mesh sieves respectively；By formula ratio, probiotics is added in resistant dextrin, adds calcium source, source of iron, zinc
Source, stachyose, oligofructose, mix, dispense up to (other formulations are pelletized as needed, tabletting, encapsulated).
Compared with prior art, the beneficial effects are mainly as follows：The present invention is according to different probiotics to calcium iron
There are the characteristics of strain differences, select to carry out the probiotics strain that calcium iron zinc-iron alloy solution has specific aim facilitation for zinc-iron alloy solution
Collocation, prescription is carried out with calcium iron zinc and prebiotics.Compared with the same type composition using plain edition probiotics strain, the combination
The absorptivity of thing calcium iron zinc is obviously improved, and calcium absorptivity improves 58.71%, and iron absorptivity improves 60.70%, zinc absorption
Improve 44.30%.
(4) embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Oligofructose molecular weight used in the embodiment of the present invention is 152.1473, and the resistant dextrin is precious raw purchased from Shandong bowling
Thing Technology Co., Ltd..
The present invention for convenience measure absorptivity, select isotope marks calcium (44Ca mark), iron (58Fe mark), zinc
(70Zn is marked) it is used as dispensing.Lactobacillus plantarum 299v is purchased from Probi companies of Sweden, and bifidobacterium longum BL88-Only is purchased from Shanghai
Jiao great Onllies limited company, animal bifidobacteria B-94 are purchased from French Lallemand USA Inc., lactobacillus plantarum ST-III is purchased from Shaanxi
Pa Neil bio tech ltd, bifidobacterium longum JDY1017 are purchased from Only Co., Ltd., Shanghai Jiantong Univ., plant breast bar
Bacterium GMNL-14, bifidobacterium longum C6, animal bifidobacteria Bb-12 are purchased from Hansen limited company of section of Denmark.
Embodiment 1：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium carbonate (with44Ca count 5g) 11.82g, ferrous gluconate (with58Fe count 0.3g) 2.32g, lemon
Sour zinc (with70Zn count 0.3g) 2.48g, probiotics 1g (lactobacillus plantarum 299v 0.5g, bifidobacterium longum BL88-Only
0.5g), stachyose 6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method：By resistant dextrin, calcium carbonate, ferrous gluconate, zinc citrate, stachyose, oligofructose point
40 mesh sieves are not crossed；By formula ratio, probiotics is added into resistant dextrin, mixes, adds calcium carbonate, ferrous gluconate, lemon
Lemon acid zinc, stachyose, oligofructose, mix, dispense up to (other formulations are pelletized as needed, tabletting, encapsulated).
Embodiment 2：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium carbonate (with44Ca count 10g) 23.64g, ferrous gluconate (with58Fe count 0.5g) 3.86g, lemon
Sour zinc (with70Zn count 0.5g) 4.14g, probiotics 2g (lactobacillus plantarum 299v 1g, bifidobacterium longum BL88-Only 1g), water
Threose 10g, oligofructose 10g, resistant dextrin 10g.
(2) preparation method is the same as embodiment 1.
Embodiment 3：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium carbonate (with44Ca count 1g) 2.36g, ferrous gluconate (with58Fe count 0.1g) 0.77g, citric acid
Zinc (with70Zn count 0.1g) 0.83g, probiotics 0.1g (lactobacillus plantarum 299v 0.05g, bifidobacterium longum BL88-Only
0.05g), stachyose 1g, oligofructose 1g, resistant dextrin 1g.
(2) preparation method is the same as embodiment 1.
Embodiment 4：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium carbonate (with44Ca count 5g) 11.82g, ferrous gluconate (with58Fe count 0.3g) 2.32g, lemon
Sour zinc (with70Zn count 0.3g) 2.48g, probiotics 1g (animal bifidobacteria B-94 0.5g, bifidobacterium longum BL88-Only
0.5g), stachyose 6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method is the same as embodiment 1.
Embodiment 5：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium gluconate (with44Ca count 5g) 11.82g, ferric pyrophosphate (with58Fe count 0.3g) 2.32g, sulfuric acid
Zinc (with70Zn count 0.3g) 2.48g, probiotics 1g (lactobacillus plantarum 299v 1g, bifidobacterium longum BL88-Only 1g), wood
Sugared 6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method is the same as embodiment 1.
Comparative example 1：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium carbonate (with44Ca count 5g) 11.82g, ferrous gluconate (with58Fe count 0.3g) 2.32g, lemon
Sour zinc (with70Zn count 0.3g) 2.48g, probiotics 1g (lactobacillus plantarum ST-III 0.5g, bifidobacterium longum JDY1017 0.5g),
Stachyose 6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method is the same as embodiment 1.
Comparative example 2：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium carbonate (with44Ca count 5g) 11.82g, ferrous gluconate (with58Fe count 0.3g) 2.32g, lemon
Sour zinc (with70Zn count 0.3g) 2.48g, probiotics 1g (0.5g lactobacillus plantarum GMNL-14,0.5g bifidobacterium longum C6), wood
Sugared 6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method is the same as embodiment 1.
Comparative example 3：Calcium, iron, zinc and probiotic composition
(1) it is formulated：Calcium gluconate (with44Ca count 5g) 11.82g, ferric pyrophosphate (with58Fe count 0.3g) 2.32g, sulfuric acid
Zinc (with70Zn count 0.3g) 2.48g, probiotics 1g (0.5g animal bifidobacteria Bb-12,0.5g bifidobacterium longum C6), stachyose
6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method is the same as embodiment 1.
Comparative example 4：Calcium, iron, Zn composition
(1) it is formulated：Calcium carbonate (with44Ca count 5g) 11.82g, ferrous gluconate (with58Fe count 0.3g) 2.32g, lemon
Sour zinc (with70Zn count 0.3g) 2.48g, stachyose 6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method：All materials are crossed 40 mesh sieves respectively according to recipe quantity to mix, mixes, dispense up to (other agent
Type is pelletized as needed, tabletting, encapsulated).
To Billy 5：Calcium, iron, Zn composition
(1) it is formulated：Calcium gluconate (with44Ca count 5g) 11.82g, ferric pyrophosphate (with58Fe count 0.3g) 2.32g, sulfuric acid
Zinc (with70Zn count 0.3g) 2.48g, stachyose 6g, oligofructose 6g, resistant dextrin 6g.
(2) preparation method：All materials are crossed 40 mesh sieves respectively according to recipe quantity to mix, mixes, dispense up to (other agent
Type is pelletized as needed, tabletting, encapsulated).
6 calcium of embodiment, iron, zinc absorption measure animal experiment
1 material and method
1.1 material
Test specimen：The composition that calcium iron zinc prepared by embodiment 1-4 is prepared with probiotic composition and comparative example 1-5
As test specimen, the sample of 0.5g/mL is made into distilled water；Using distilled water as control sample；
Experimental animal：Cleaning grade ICR mouse 100, half male and half female, 18~22g of weight.
1.2 method
1.2.1 packet：All mouse are randomly divided into 10 groups, every group 10, half male and half female, it is pair respectively to organize 1- groups 5
Be experimental group (sample of embodiment 1-4 preparations) according to group (comparative example 1-5 prepare sample), group 6- groups 9, to organize 10 be blank control
Group (distilled water).
1.2.2 feeding method：Control group mice feeds examination prepared by the comparative example 1- comparative examples 5 of 10mL/kg weight daily
Sample；Experimental mice feeds sample prepared by the embodiment 1-4 of 10mL/kg weight daily.Blank control group feeds 10mL/ daily
The distilled water of kg weight.1 times/day, continuous 30 days.All mouse feed normal diet 6g daily, 3 times/day.
1.2.3 the collection of excrement：In experiment the 1st day, last 1 day of experiment, excrement label light blue (food-grade is given respectively
Light blue) 0.01g, it is mixed in sample and is fed.It is baseline sample that the sample before light blue, which occurs, in stool in mice；When in stool in mice
There is light blue for the first time, be experimental period sample to the fecal specimens for occurring light blue for the second time；There is light blue for the second time in stool in mice
Fecal specimens afterwards are sample after experiment.Collect and weigh respectively, record.By all experimental period samples, according to mass ratio 1:2
Ratio add ultrapure water homogenisation.
1.2.4 sample analysis：Fecal specimens after homogeneous carry out vacuum freeze drying after 119 DEG C of 15min sterilize, and claim
Weight, crushes, and measures the abundance of stable isotope.
(1) measure of calcium in excrement, iron, zinc and dysprosium content：Weighing about 0.5g experimental periods dries fecal specimens in crucible,
Dry ashing, 25mL is settled to the HCl/water solution of 0.5mol/L, is treatments of the sample liquid.With thermoionization mass spectrograph
(TIMS, Finigan MAT THQ) and inductive couple plasma mass spectrometer (ICP-MS, Perkin-Elmer Elan
6000) content of isotope calcium in fecal specimens, iron isotope, isotope zinc and dysprosium is measured respectively, and the rubidium using 20ng/g is interior
Mark thing.
(2) measure of calcium isotope：Step (1) 5mL treatments of the sample liquid is taken, after centrifuging (5000r/min, 20min), on
The HNO of clear liquid volumetric concentration 2%3Aqueous solution is settled to 25mL.With thermoionization mass spectrograph (TIMS, Finigan MAT
THQ calcium isotope abundance) is measured.
(3) measure of zinc isotope：Step (1) 5mL treatments of the sample liquid is taken, after centrifuging (5000r/min, 20min), on
The HNO of clear liquid volumetric concentration 2%3Aqueous solution is settled to 25mL.With inductive couple plasma mass spectrometer (ICP-MS,
Perkin-Elmer Elan 6000) measure zinc isotope abundance.
(4) measure of iron isotope：Experimental period drying fecal specimens about 0.5g is weighed, adds the top pure grade HNO of 5mL3,
The pure H of analysis of 2mL2O2, microwave digestion (200 DEG C, 500w, which is digested to liquid, to be clarified).Digestive juice is settled to 25mL with ultra-pure water, uses
Inductive couple plasma mass spectrometer (ICP-MS, Perkin-Elmer Elan 6000) method measures iron isotope abundance.
(5) calculating of calcium, iron, zinc apparent absorptivity：Absorptivity=(oral isotope dose-excrement discharge isotope agent
Amount)/oral isotope dose × 100%, the constituent content in excrement discharge isotope dose=excrement × (isotope abundance-
Baseline isotope abundance), corrected with Dy discharge rates：Correct absorptivity={ 1- [(1- absorptivities)/Dy rate of recovery] } × 100%.
Test data (P as shown in table 1<0.05),For mean+SD.
2 results
The results are shown in Table 1, and all test group calcium, iron, the absorptivity of zinc are obviously higher than contrast groups.Feed embodiment
The mouse calcium, iron, the absorptivity highest of zinc of 1 sample and 4 sample of embodiment, illustrate that absorption of the probiotics to calcium iron zinc has and determine
Effect.Embodiment 2,3 results relatively show that the combination of identical supplementary material different proportion, the absorption of calcium iron zinc has compared with Example 1
Certain difference.
Comparative example 1,2 is only the bacterial strain difference of probiotics compared with Example 1, but the calcium iron zinc-iron alloy solution effect of the two
Fruit but well below embodiment 1, illustrates significant difference of the bacterial strain to promotion calcium iron zinc-iron alloy solution；Equally, comparative example 3 and embodiment 4
Result also embody the significance of difference of the bacterial strain to calcium iron zinc-iron alloy solution.And comparative example 4,5 as a result, compared with embodiment, into
One step illustrates facilitation of the probiotics to calcium iron zinc-iron alloy solution.
These data illustrate the specific bacterial strain of selected probiotics, and the absorption to calcium, iron, zinc, which has, is obviously promoted effect,
And different probiotics strains are sufficient into effect there are different forward directions to the absorptivity of mouse calcium, iron, zinc.
1 mouse of table is in test specimen44Ca、58Fe、70The absorptivity of Zn
Note：The * compared with contrast groups group：P<0.05.
3 conclusions
Using mouse as subjects, this product 1 month of various dose is given by oral cavity, the results show that this product has
Play the role of supplementing calcium, iron, zinc and improve calcium, iron, zinc absorption.Compared with comparative example, calcium absorptivity improves 58.71%, iron
Absorptivity improves 60.70%, and zinc absorption improves 44.30%.
This research discloses and adds probiotics in calcium, iron, Zinc supplements, can be obviously promoted absorption of the mouse to calcium, iron, zinc
Rate, and different probiotics have differences the absorption aspects for promoting calcium, iron, zinc.Carried in short, data above shows that said preparation has
Effect of the high mouse to calcium, iron, zinc absorption.Result, it is believed that this product has the function of significantly to promote calcium, iron, zinc-iron alloy solution.
Claims (9)
1. a kind of calcium iron zinc and probiotic composition, it is characterised in that the composition is made of the raw material of following weight proportion：
1~10 part of calcium source, 0.1~0.5 part of source of iron, 0.1~0.5 part of zinc source, 0.1~2 part of probiotics, 1~10 part of stachyose, oligomeric fruit
Sugared 1~10 part, 1~10 part of resistant dextrin；The probiotics is lactobacillus plantarum, bifidobacterium longum, animal bifidobacteria or thermophilic
One or more of combinations in hot streptococcus；The calcium source dosage is with calcareous gauge, and the source of iron dosage is with irony gauge, institute
Zinc source dosage is stated in terms of zinc quality.
2. calcium iron zinc as claimed in claim 1 and probiotic composition, it is characterised in that the calcium source for calcium carbonate, calcium acetate,
Calcium chloride, calcium gluconate, calcium citrate, calcium lactate, calcium monohydrogen phosphate, calcium dihydrogen phosphate, calcium phosphate, calcium sulfate, L-calcium lactate
Or the combination of one or more of arbitrary proportions in calcium glycerophosphate.
3. calcium iron zinc as claimed in claim 1 and probiotic composition, it is characterised in that the source of iron is ferrous gluconate, richness
The combination of horse acid ferrous iron, ferric pyrophosphate, ferrous sulfate, ferrous lactate or one or more of arbitrary proportions in ferrous succinate.
4. calcium iron zinc as claimed in claim 1 and probiotic composition, it is characterised in that the zinc source is zinc sulfate, citric acid
The combination of one or more of arbitrary proportions in zinc, zinc gluconate, zinc oxide or zinc lactate.
5. calcium iron zinc as claimed in claim 1 and probiotic composition, it is characterised in that the probiotics is lactobacillus plantarum
Any ratio of one or more in 299v, bifidobacterium longum BL88-Only, animal bifidobacteria B-94 or streptococcus thermophilus ST-6
The mixing of example.
6. calcium iron zinc as claimed in claim 5 and probiotic composition, it is characterised in that the probiotics is one of following：(1)
Lactobacillus plantarum 299v and bifidobacterium longum BL88-Only is with mass ratio 1:1 mixing；(2) lactobacillus plantarum 299v and animal
Bifidobacterium B-94 is with mass ratio 1:1 mixing；(3) lactobacillus plantarum 299v and streptococcus thermophilus ST-6 is with mass ratio 1:1
Mixing；(4) animal bifidobacteria B-94 and bifidobacterium longum BL88-Only is with mass ratio 1:1 mixing.
7. calcium iron zinc as claimed in claim 6 and probiotic composition, it is characterised in that the composition is by following weight proportion
Raw material composition：It is 10 parts of calcium source, 0.5 part of source of iron, 0.5 part of zinc source, 2 parts of probiotics, 10 parts of stachyose, 10 parts of oligofructose, anti-
10 parts of dextrin of property；The calcium source is calcium carbonate or calcium gluconate；The source of iron is ferrous gluconate or ferric pyrophosphate；It is described
Zinc source is zinc sulfate or zinc citrate；The probiotics is lactobacillus plantarum 299v and bifidobacterium longum BL88-Only with quality
Than 1:1 mixing.
8. calcium iron zinc described in a kind of claim 1 and application of the probiotic composition in food additives are prepared.
9. application as claimed in claim 8, it is characterised in that the food additives are pulvis, granule, tablet or capsule
Agent.
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| CN112401247A (en) * | 2019-08-23 | 2021-02-26 | 河南诺美药业有限公司 | Fruity calcium acetate preparation granule and preparation method thereof |
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