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CN108013239A - A kind of nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently - Google Patents

A kind of nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently Download PDF

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Publication number
CN108013239A
CN108013239A CN201810053863.3A CN201810053863A CN108013239A CN 108013239 A CN108013239 A CN 108013239A CN 201810053863 A CN201810053863 A CN 201810053863A CN 108013239 A CN108013239 A CN 108013239A
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fermentation
endoparasite
liquid
sheep
fermented feed
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黄小平
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Fujian Wei Key Technology Incubator Co Ltd
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Fujian Wei Key Technology Incubator Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/288—Taraxacum (dandelion)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/33—Cactaceae (Cactus family), e.g. pricklypear or Cereus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/42—Cucurbitaceae (Cucumber family)
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/758—Zanthoxylum, e.g. pricklyash
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/888—Araceae (Arum family), e.g. caladium, calla lily or skunk cabbage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a kind of nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently, it is related to biological feedstuff technical field, includes the following steps：(1) base-material that ferments prepares, prepared by (2) zymocyte liquid, (3) fermentation base-material inoculation, (4) solid state fermentation, (5) drying granulation.The present invention makes made nutrition fermented feed assign its excellent sheep endoparasite prophylactic-therapeutic effect while sheep basal nutrient demand is met by the addition of endoparasite control agent, especially the use of Chinese herb prevention agent can significantly improve the endoparasite prevention effect to flock of sheep, and prevent using easy Problems Concerning Their Recurrence existing for conventional control agent ivermectin, albendazole, levamisol.

Description

A kind of nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently
Technical field：
The present invention relates to biological feedstuff technical field, and in particular to a kind of nutrition for having sheep endoparasite prophylactic-therapeutic effect concurrently Fermented feed processing method.
Background technology：
Fermented feed, refers to that roughage undergoes microbial fermentation and manufactured feed.Rich in cellulose, half fiber in roughage Crude fibre and the protein such as element, pectin substance, lignin are tieed up, but is difficult to directly be digested and assimilated by animal, animal, which has eaten, to be increased Enteron aisle is born, and causes intestines problem.And fermented feed can not only make up the amino acid easily lacked in conventional feed, Er Qieneng Other roughage raw material nutritions is converted rapidly, reach enhancing and digest and absorb effect.
Sheep endoparasite mainly has roundworm, pinworm, tapeworm, whipworm, oesophagostome etc., and these types of parasite is to sheep body Harm it is big, adult and sheep contention nutritional ingredient, move intestinal wall, liver and lung tissue structure and physiology machine that shape larva destroys sheep Can, cause the daily gain of sheep to reduce, the problems such as premunition declines.At present, the medicine of western medical treatment parasitic disease mainly has： The a series of insecticide such as ivermectin, avermectin, albendazole, mebendazole, levamisol, piperazine citrate, still There is certain limitation.At present, anthelmintic can only be effective for one or two kinds of parasite, and has certain side effect, It is unfavorable for the healthy growth of sheep.
The content of the invention：
The technical problems to be solved by the invention are that providing one kind advantageously reduces endoparasite incidence and carry The nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently of high endoparasite control agent bioavilability.
The technical problems to be solved by the invention are realized using following technical solution：
A kind of nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently, includes the following steps：
(1) base-material that ferments prepares：Water proof boiling 30-60min after rice bran and rapeseed dregs are mixed, dries in the air cool rear red with chopping Potato seedling, bagasse and brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and fermentation base-material is made；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid 3-5 by volume:2-3:1-2:0.5-1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2-5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 3-5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300-500r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Rice bran, rapeseed dregs, sweet potato vine, bagasse, the mass ratio of brewex's grains are 40-60 in the step (1):30-50: 15-25:10-20:5-10。
Bacillus subtilis bacterium solution concentration 1 × 10 in the step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
Endoparasite control agent additive amount is the 0.5-1wt% of fermentate dry weight in the step (5).
The one kind of endoparasite control agent in ivermectin, albendazole, levamisol in the step (5).
Endoparasite control agent is made of Chinese medicine freeze-dried powder and starch carrier in the step (5), and its preparation method is： First starch carrier is dissolved in 70% ethanol saturation carrier solution is made, then carrier solution is added into Chinese medicine freeze-dried powder, fully Mixing, gained mixture are sent into 50-55 DEG C of baking oven and are dried to constant weight, powder most are made through pulverizer afterwards, up to endobiosis Worm control agent.
The starch carrier, the mass ratio of Chinese medicine freeze-dried powder are 5-10:5-10.
The starch carrier is formed by starch conversion, and its preparation method is：Starch plus water are made into the starch of concentration 30% Slurry, adds food-grade polyglutamic acid, and be heated to 40-50 DEG C to 8-9 with 3% sodium hydroxide solution regulation system pH value Insulated and stirred is reacted, and reacting liquid pH value is adjusted to neutrality, centrifugal filtration with 3% hydrochloric acid solution after reaction, gained filter residue is sent Enter in freeze drier, it is freeze-dried to constant weight, and powder is made through pulverizer, up to starch carrier.
The starch, the mass ratio of food-grade polyglutamic acid are 20-30:5-10.
The Chinese medicine freeze-dried powder is made of the Chinese medicine of following weight：Dandelion 5-10g, bunge pricklyash leaf 5-10g, shepherd's purse 3-5g, Purslane 3-5g, taro leaf 1-2g, ginkgo leaf 1-2g, balsampear leaf 1-2g, cacumen biotae 0.5-1g, cactus 0.5-1g, semen abutili 0.1-0.5g, its preparation method are：The above-mentioned Chinese medicine of fresh collection is sent into freeze drier, it is freeze-dried to constant weight, And powder is made through pulverizer, up to Chinese medicine freeze-dried powder.
Chinese medicine character and effect：
Dandelion：It is bitter, sweet, it is cold in nature, there is the effect of clearing heat and detoxicating, diuresis dissipating bind, monarch drug in a prescription.
Bunge pricklyash leaf：Acrid flavour, property heat, has the effect of analgesic, desinsection, monarch drug in a prescription.
Shepherd's purse：It is sweet in flavor, it is mild-natured, there is the effect of with spleen, Li Shui, hemostasis, improving eyesight, ministerial drug.
Purslane：It is sour, it is cold in nature, there is inducing diuresis for removing edema, blood pressure lowering, eliminate toxic dust, prevention ulcer, the work(of bactericidal antiphlogistic Effect, ministerial drug.
Taro leaf：It is acrid flavour, sweet, it is mild-natured, for diarrhea, spontaneous perspiration, night sweat, swollen ulcer drug, impetigo, snakebite and bugbite, ministerial drug.
Ginkgo leaf：It is sweet in flavor, bitter, puckery, it is mild-natured, have it is promoting blood circulation and removing blood stasis, the effect of removing obstruction in channels to relieve pain, astringe the lung and relieving asthma, change turbid lipid-loweringing, Adjutant.
Balsampear leaf：Bitter, it is cool in nature, there is the effect of clearing heat and detoxicating, adjutant.
Cacumen biotae：It is bitter, puckery, it is cold in nature, there is the effect of cooling blood and hemostasis, preventing phlegm from forming and stopping coughing, growing and blacking hair, adjutant.
Cactus：Bitter, it is cold in nature, there is the effect of promoting flow of qi and blood circulation, clearing heat and detoxicating, make medicine.
Semen abutili：Bitter, it is mild-natured, there is clearing heat and promoting diuresis, detoxify, move back the effect of screen, make medicine.
The beneficial effects of the invention are as follows：The present invention makes made nutrition fermented feed by the addition of endoparasite control agent Assign its excellent sheep endoparasite prophylactic-therapeutic effect while sheep basal nutrient demand is met, especially Chinese herb prevention agent makes With the endoparasite prevention effect that can be significantly improved to flock of sheep, and prevent using conventional control agent ivermectin, albendazole, Easy Problems Concerning Their Recurrence existing for levamisol；Chinese medicine freeze-dried powder in Chinese herb prevention agent prepares the monarch compatibility pass for following Chinese medicine System, and gently, have no toxic side effect；When being added with the stop for being beneficial to extension Chinese herb prevention component in sheep body of starch carrier Between, the bioavailability of effective component is improved, so as to strengthen prevention effect.
Embodiment：
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention easy to understand, tie below Specific embodiment is closed, the present invention is further explained.
Embodiment 1
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
In step (5) endoparasite control agent additive amount be fermentate dry weight 0.5wt%, endoparasite control agent Selected from ivermectin.
Embodiment 2
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
In step (5) endoparasite control agent additive amount be fermentate dry weight 0.5wt%, endoparasite control agent Selected from albendazole.
Embodiment 3
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
In step (5) endoparasite control agent additive amount be fermentate dry weight 0.5wt%, endoparasite control agent Selected from levamisol.
Embodiment 4
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
In step (5) endoparasite control agent additive amount be fermentate dry weight 0.5wt%, endoparasite control agent Preparation：First 10g starch carriers are dissolved in 70% ethanol saturation carrier solution is made, then added and carry into 5g Chinese medicine freeze-dried powders Liquid solution, is sufficiently mixed, and gained mixture is sent into 50-55 DEG C of baking oven and is dried to constant weight, powder most is made through pulverizer afterwards, i.e., Obtain endoparasite control agent.
The preparation of starch carrier：Add water to be made into the starch slurry of concentration 30% 25g cornstarch, it is more to add 5g food-grades Polyglutamic acid, and with 3% sodium hydroxide solution regulation system pH value to 8-9, it is heated to 40-50 DEG C of insulated and stirred reaction, reaction After with 3% hydrochloric acid solution reacting liquid pH value is adjusted to neutrality, centrifugal filtration, gained filter residue is sent into freeze drier, warp Powder is made to constant weight, and through pulverizer in freeze-drying, up to starch carrier.
The preparation of Chinese medicine freeze-dried powder：By the 10g dandelions of fresh collection, 8g bunge pricklyash leafs, 5g shepherd's purses, 3g purslanes, 2g taros Leaf, 2g ginkgo leaves, 1g balsampear leafs, 0.5g cacumen biotaes, 0.5g cactuses, 0.2g semen abutilis are sent into freeze drier, chilled It is dry that powder is made to constant weight, and through pulverizer, up to Chinese medicine freeze-dried powder.
Embodiment 5
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
In step (5) endoparasite control agent additive amount be fermentate dry weight 0.5wt%, endoparasite control agent Preparation：First 5g starch carriers are dissolved in 70% ethanol saturation carrier solution is made, then added and carry into 5g Chinese medicine freeze-dried powders Liquid solution, is sufficiently mixed, and gained mixture is sent into 50-55 DEG C of baking oven and is dried to constant weight, powder most is made through pulverizer afterwards, i.e., Obtain endoparasite control agent.
The preparation of starch carrier：Add water to be made into the starch slurry of concentration 30% 25g cornstarch, it is more to add 5g food-grades Polyglutamic acid, and with 3% sodium hydroxide solution regulation system pH value to 8-9, it is heated to 40-50 DEG C of insulated and stirred reaction, reaction After with 3% hydrochloric acid solution reacting liquid pH value is adjusted to neutrality, centrifugal filtration, gained filter residue is sent into freeze drier, warp Powder is made to constant weight, and through pulverizer in freeze-drying, up to starch carrier.
The preparation of Chinese medicine freeze-dried powder：By the 10g dandelions of fresh collection, 8g bunge pricklyash leafs, 5g shepherd's purses, 3g purslanes, 2g taros Leaf, 2g ginkgo leaves, 1g balsampear leafs, 0.5g cacumen biotaes, 0.5g cactuses, 0.2g semen abutilis are sent into freeze drier, chilled It is dry that powder is made to constant weight, and through pulverizer, up to Chinese medicine freeze-dried powder.
Reference examples 1
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
In step (5) endoparasite control agent additive amount be fermentate dry weight 0.5wt%, endoparasite control agent Preparation：First 5g cornstarch is dissolved in 70% ethanol saturation carrier solution is made, then added and carry into 5g Chinese medicine freeze-dried powders Liquid solution, is sufficiently mixed, and gained mixture is sent into 50-55 DEG C of baking oven and is dried to constant weight, powder most is made through pulverizer afterwards, i.e., Obtain endoparasite control agent.
The preparation of Chinese medicine freeze-dried powder：By the 10g dandelions of fresh collection, 8g bunge pricklyash leafs, 5g shepherd's purses, 3g purslanes, 2g taros Leaf, 2g ginkgo leaves, 1g balsampear leafs, 0.5g cacumen biotaes, 0.5g cactuses, 0.2g semen abutilis are sent into freeze drier, chilled It is dry that powder is made to constant weight, and through pulverizer, up to Chinese medicine freeze-dried powder.
Reference examples 2
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite prevention after crushed Agent, is uniformly mixed, 50-70 mesh particles finally is made using granulator, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
In step (5) endoparasite control agent additive amount be fermentate dry weight 0.5wt%, endoparasite control agent Preparation：First 5g starch carriers are dissolved in 70% ethanol saturation carrier solution is made, then added and carry into 5g Chinese medicine additives Liquid solution, is sufficiently mixed, and gained mixture is sent into 50-55 DEG C of baking oven and is dried to constant weight, powder most is made through pulverizer afterwards, i.e., Obtain endoparasite control agent.
The preparation of starch carrier：Add water to be made into the starch slurry of concentration 30% 25g cornstarch, it is more to add 5g food-grades Polyglutamic acid, and with 3% sodium hydroxide solution regulation system pH value to 8-9, it is heated to 40-50 DEG C of insulated and stirred reaction, reaction After with 3% hydrochloric acid solution reacting liquid pH value is adjusted to neutrality, centrifugal filtration, gained filter residue is sent into freeze drier, warp Powder is made to constant weight, and through pulverizer in freeze-drying, up to starch carrier.
Chinese medicine additive selects a kind of Chinese herbal feeds for being used to prevent sheep endoparasite of patent CN 106729054A to add Add 5 made Chinese herbal feed additive of agent embodiment.
Blank example
(1) base-material that ferments prepares：Water proof boiling 1h after 50kg rice brans and 35kg rapeseed dregs are mixed, dries in the air cool rear and chopping 20kg sweet potato vines, 15kg bagasse and 5kg brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and hair is made Ferment base-material；
(2) prepared by zymocyte liquid：By bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, S. cervisiae Liquid by volume 5:3:2:1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2.5wt%, and adds water to adjust water content and reaches To 60-70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in 30-40 DEG C of fermentation temperature Lower fermentation 5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and 50-70 is made using granulator after crushed Mesh particle, up to nutrition fermented feed.
Wherein, bacillus subtilis bacterium solution concentration 1 × 10 in step (2)10-2×1010CFU/mL, bacillus licheniformis liquid are dense Degree 4 × 109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108- 8×108CFU/mL。
Embodiment 6
It is utilized respectively embodiment 1-5, reference examples 1-2, blank example and prepares nutrition fermented feed.800 weight are chosen in 15- The sheep of 20kg, and stochastic averagina is divided into 8 groups, and embodiment 1-5, reference examples 1-2, the made battalion of blank example are fed respectively to this 8 groups of sheep Support fermented feed 3 months, measure the bioavilability of every group of sheep endoparasite incidence and endoparasite control agent, knot Fruit is as shown in table 1.
Prevention effect of the made nutrition fermented feed of the present invention of table 1 to endoparasite
Group Endoparasite incidence/% Endoparasite control agent bioavilability/%
Embodiment 1 1 82.4
Embodiment 2 0 80.6
Embodiment 3 1 84.2
Embodiment 4 0 96.8
Embodiment 5 0 97.3
Reference examples 1 0 89.7
Reference examples 2 3 78.1
Blank example 28 -
The prevention effect of the application Chinese medicine endoparasite control agent is substantially better than conventional western medicine ivermectin, acetysalicylic acid phenobarbital reaches Azoles, levamisol and the Chinese herb prevention agent reported, and being used cooperatively by starch carrier, significantly improve endobiosis The bioavilability of worm control agent.
The basic principles, main features and the advantages of the invention have been shown and described above.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its Equivalent thereof.

Claims (5)

1. a kind of nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently, it is characterised in that including as follows Step：
(1) base-material that ferments prepares：Water proof boiling 30-60min after rice bran and rapeseed dregs are mixed, the cool rear sweet potato with chopping of drying in the air Seedling, bagasse and brewex's grains are sufficiently mixed, and are added water to adjust water content and reached 40-50wt%, and fermentation base-material is made；
(2) prepared by zymocyte liquid：Bacillus subtilis bacterium solution, bacillus licheniformis liquid, Bifidobacterium liquid, saccharomyces cerevisiae bacterium solution are pressed Volume ratio 3-5:2-3:1-2:0.5-1 is mixed to prepare zymocyte liquid；
(3) base-material that ferments is inoculated with：Zymocyte liquid is inoculated with fermentation base-material by 2-5wt%, and adds water to adjust water content and reaches 60- 70wt%, up to mixture；
(4) solid state fermentation：Fill this blend into solid-state fermenter and be sealed by fermentation, and in fermentation temperature 30-40 DEG C issue Ferment 3-5 days, a few days ago static fermentation, starts stirring fermentation, rotating speed 300-500r/min on the 3rd day；
(5) drying granulation：Gained fermentate is dried after the completion of fermentation, and is mixed into endoparasite control agent after crushed, is mixed Close uniformly, 50-70 mesh particles finally are made using granulator, up to nutrition fermented feed.
2. the nutrition fermented feed processing method according to claim 1 for having sheep endoparasite prophylactic-therapeutic effect concurrently, it is special Sign is：Rice bran, rapeseed dregs, sweet potato vine, bagasse, the mass ratio of brewex's grains are 40-60 in the step (1):30-50:15- 25:10-20:5-10。
3. the nutrition fermented feed processing method according to claim 1 for having sheep endoparasite prophylactic-therapeutic effect concurrently, it is special Sign is：Bacillus subtilis bacterium solution concentration 1 × 10 in the step (2)10-2×1010CFU/mL, bacillus licheniformis liquid concentration 4×109-5×109CFU/mL, Bifidobacterium liquid concentration 2 × 109-3×109CFU/mL, saccharomyces cerevisiae bacterium solution concentration 7 × 108-8 ×108CFU/mL。
4. the nutrition fermented feed processing method according to claim 1 for having sheep endoparasite prophylactic-therapeutic effect concurrently, it is special Sign is：Endoparasite control agent additive amount is the 0.5-1wt% of fermentate dry weight in the step (5).
5. the nutrition fermented feed processing method according to claim 1 for having sheep endoparasite prophylactic-therapeutic effect concurrently, it is special Sign is：The one kind of endoparasite control agent in ivermectin, albendazole, levamisol in the step (5).
CN201810053863.3A 2018-01-19 2018-01-19 A kind of nutrition fermented feed processing method for having sheep endoparasite prophylactic-therapeutic effect concurrently Pending CN108013239A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1214909A (en) * 1998-05-14 1999-04-28 王玉万 Complex anti-parasite medicine for animal use
CN1460485A (en) * 2003-03-27 2003-12-10 中牧实业股份有限公司 New process for making animal medicine madumycin ammonium, ivermection or dikezhuli premixed agent
CN1589155A (en) * 2001-10-05 2005-03-02 鲁比康科学有限公司 Animal feeds including actives and methods of using the same
CN103445033A (en) * 2013-09-26 2013-12-18 张娥珍 Method and product for producing mutton sheep fully-mixed pellet feed by using sugarcane tail tips
CN104397347A (en) * 2014-12-23 2015-03-11 中粮集团有限公司 Fermented feed containing rapeseed meal, and preparation method of fermented feed
CN106721140A (en) * 2016-11-25 2017-05-31 周福弟 It is a kind of raise feeder for goat and goat fatten method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1214909A (en) * 1998-05-14 1999-04-28 王玉万 Complex anti-parasite medicine for animal use
CN1589155A (en) * 2001-10-05 2005-03-02 鲁比康科学有限公司 Animal feeds including actives and methods of using the same
CN1460485A (en) * 2003-03-27 2003-12-10 中牧实业股份有限公司 New process for making animal medicine madumycin ammonium, ivermection or dikezhuli premixed agent
CN103445033A (en) * 2013-09-26 2013-12-18 张娥珍 Method and product for producing mutton sheep fully-mixed pellet feed by using sugarcane tail tips
CN104397347A (en) * 2014-12-23 2015-03-11 中粮集团有限公司 Fermented feed containing rapeseed meal, and preparation method of fermented feed
CN106721140A (en) * 2016-11-25 2017-05-31 周福弟 It is a kind of raise feeder for goat and goat fatten method

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