CN107986456A - A kind of prevention and suppressing method applied to water body cyanobacteria - Google Patents
A kind of prevention and suppressing method applied to water body cyanobacteria Download PDFInfo
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- CN107986456A CN107986456A CN201711334524.4A CN201711334524A CN107986456A CN 107986456 A CN107986456 A CN 107986456A CN 201711334524 A CN201711334524 A CN 201711334524A CN 107986456 A CN107986456 A CN 107986456A
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- prevention
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- chlorella
- cyanobacteria
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 241000192700 Cyanobacteria Species 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000002265 prevention Effects 0.000 title claims abstract description 30
- 241000894006 Bacteria Species 0.000 claims abstract description 67
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 46
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 36
- 239000003643 water by type Substances 0.000 claims abstract description 33
- 241000195493 Cryptophyta Species 0.000 claims abstract description 17
- 239000007921 spray Substances 0.000 claims abstract description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 8
- 238000012545 processing Methods 0.000 claims abstract description 6
- 238000005507 spraying Methods 0.000 claims abstract description 6
- 238000001514 detection method Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 47
- 238000010790 dilution Methods 0.000 claims description 26
- 239000012895 dilution Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 26
- 239000001963 growth medium Substances 0.000 claims description 25
- 229920001817 Agar Polymers 0.000 claims description 21
- 239000008272 agar Substances 0.000 claims description 21
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 244000063299 Bacillus subtilis Species 0.000 claims description 17
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 17
- 241001567730 Mrakia aquatica Species 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 16
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical group [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 16
- 241000589614 Pseudomonas stutzeri Species 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 13
- 230000001954 sterilising effect Effects 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000008280 blood Substances 0.000 claims description 12
- 210000004369 blood Anatomy 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- 239000010902 straw Substances 0.000 claims description 12
- 235000002639 sodium chloride Nutrition 0.000 claims description 11
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 10
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 10
- 229920002472 Starch Polymers 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 9
- 239000002068 microbial inoculum Substances 0.000 claims description 9
- 239000008107 starch Substances 0.000 claims description 9
- 235000019698 starch Nutrition 0.000 claims description 9
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 8
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 229910016374 CuSO45H2O Inorganic materials 0.000 claims description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000009472 formulation Methods 0.000 claims description 6
- 238000000386 microscopy Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 238000012856 packing Methods 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 5
- 239000011324 bead Substances 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- 229960002413 ferric citrate Drugs 0.000 claims description 5
- 239000013505 freshwater Substances 0.000 claims description 5
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 5
- 235000017550 sodium carbonate Nutrition 0.000 claims description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 5
- IICCLYANAQEHCI-UHFFFAOYSA-N 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodospiro[2-benzofuran-3,9'-xanthene]-1-one Chemical compound O1C(=O)C(C(=C(Cl)C(Cl)=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 IICCLYANAQEHCI-UHFFFAOYSA-N 0.000 claims description 3
- -1 NaH2PO42H2O Chemical compound 0.000 claims description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 239000012267 brine Substances 0.000 claims description 3
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 3
- 229960005091 chloramphenicol Drugs 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 3
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 235000010333 potassium nitrate Nutrition 0.000 claims description 3
- 239000004323 potassium nitrate Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 229930187593 rose bengal Natural products 0.000 claims description 3
- 229940081623 rose bengal Drugs 0.000 claims description 3
- STRXNPAVPKGJQR-UHFFFAOYSA-N rose bengal A Natural products O1C(=O)C(C(=CC=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 STRXNPAVPKGJQR-UHFFFAOYSA-N 0.000 claims description 3
- 239000013535 sea water Substances 0.000 claims description 3
- 235000010344 sodium nitrate Nutrition 0.000 claims description 3
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 3
- 238000007711 solidification Methods 0.000 claims description 3
- 230000008023 solidification Effects 0.000 claims description 3
- 239000011592 zinc chloride Substances 0.000 claims description 3
- 235000005074 zinc chloride Nutrition 0.000 claims description 3
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 3
- 239000008121 dextrose Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 14
- 230000009286 beneficial effect Effects 0.000 abstract description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 7
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 7
- 239000011574 phosphorus Substances 0.000 abstract description 7
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 7
- 239000005416 organic matter Substances 0.000 abstract description 5
- 238000001035 drying Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241000192710 Microcystis aeruginosa Species 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- SRUWWOSWHXIIIA-UKPGNTDSSA-N Cyanoginosin Chemical compound N1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](C)[C@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C(=C)N(C)C(=O)CC[C@H](C(O)=O)N(C)C(=O)[C@@H](C)[C@@H]1\C=C\C(\C)=C\[C@H](C)[C@@H](O)CC1=CC=CC=C1 SRUWWOSWHXIIIA-UKPGNTDSSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 108010067094 microcystin Proteins 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 241000192542 Anabaena Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010049746 Microcystins Proteins 0.000 description 1
- 229910004619 Na2MoO4 Inorganic materials 0.000 description 1
- 241000192656 Nostoc Species 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical class C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/20—Prevention of biofouling
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to cyanobacteria prevention and processing applied technical field, specifically disclose a kind of prevention applied to water body cyanobacteria and suppressing method, step 1, determine to apply the waters of bioactivity bacteria agent product first, recycles sprinkling machinery that bioactivity bacteria agent is uniformly applied to pending waters.Step 2, be monitored pending waters, 78 it is small when after, then pending waters pass through spray machinery apply chlorella solution.Step 3, be monitored the waters for spraying chlorella solution, according to the cyanobacteria state of detection, repeat step 1, step 2.The beneficial effects of the present invention are：Pass through the bioactivity bacteria agent of high-content, energy fast decoupled applies the organic matter in waters, application is supplemented at the same time beneficial to chlorella solution, beneficial bacterium is set quickly to absorb the nutriment in applied waters, so as to quickly breed, harmful algae living space is reduced high-content bacillus and decompose organic matter, nitrogen in waters, phosphorus ratio are applied in adjusting, realize prevention and the purpose administered.
Description
Technical field
The invention belongs to cyanobacteria prevention and processing applied technical field, and in particular to a kind of prevention applied to water body cyanobacteria
And suppressing method.
Background technology
Cyanobacteria is prokaryotes, is called blue-green alge or cyanobacteria；There is colloid clothing outside the cell membrane of most of cyanobacterias, be called
Myxophyceae.In algae, cyanobacteria is most simple, the unicellular organism of most original, such as Microcystis aeruginosa, nostoc, anabena.Cyanobacteria does not have
There is nucleus, there is nuclear matter in cell center, is usually in granular form or netted, and pigment is evenly distributed in cytoplasm, nuclear matter
There is no nuclear membrane and kernel, there is core, therefore referred to as protokaryon（Or nucleoid）, have cyclic DNA plasmid in cyanobacteria, take on carrier
Effect.
In some water bodys full of nutrition, some cyanobacterias often in summer amount reproduction, and the water surface formed one layer it is bluish-green
Color and the offscum for having bad smell, are known as " wawter bloom ", large-scale blue algae bloom, is referred to as " green tide "（The red tide occurred with ocean
It is corresponding）.Green tide causes water quality deterioration, exhausts water oxygen when serious and causes the death of fish, more seriously, in cyanobacteria
Some species（Such as Microcystis aeruginosa）Microcystin can also be produced（Microcystins, abbreviation MCs）, contain in about 50% green tide
There are a large amount of MCs.MCs to fish, people and animals in addition to directly producing murder by poisoning, and the major incentive of liver cancer.MCs is heat-resisting, is not easy
Decomposed by boiling water, but can be by active carbon absorption, it is possible to contaminated water source is purified with activated carbon water purifier.
When cyanobacteria largely occurs, neighbouring water body is generally covered in blueness or green, the water surface by thick blue-green water bloom,
Accumulated by wind to bank, can not only give out a foul smell taste, and the Cells of Blue-green Algae containing toxin floats in water body, when with some suspensions
Thing complex-precipitation, or precipitated after being preyed on by cultivation object with its excreta, in fishpond, bottom of pond is enriched with, and nuisanceless aquatic products are produced
Huge negative effect can be brought.
Anabaena in cyanobacteria can quickly produce lethal factor, destroy the gill tissue of cultivation object, disturb its metabolism
Be normally carried out, paralysis nerve, makes its dead, indivedual kinds not only live body bands poison, and dead individuals are decomposed and can produced in cyanobacteria
Biotoxin-cyanophycean toxin（Such as Microcystin）.Cyanophycean toxin amount can directly contribute cultivation object and be poisoned to death when more；Or
Even if person's quantity is few, cultivation object can be also endangered by food chain build-up effect, until harmful to human.
Therefore, a kind of prevention and suppressing method applied to water body cyanobacteria is provided based on the above problem, the present invention.
The content of the invention
Goal of the invention：, can be to not the object of the present invention is to provide a kind of prevention applied to water body cyanobacteria and suppressing method
The waters that cyanobacteria occurs is prevented, the waters that cyanobacteria has occurred is carried out efficiently, the processing stablized and without any negative effect,
Ensure the safety of water body environment.
Technical solution：The present invention provides a kind of prevention and suppressing method applied to water body cyanobacteria, comprises the following steps, and walks
Rapid 1, the waters of application bioactivity bacteria agent product is determined first, recycles sprinkling machinery uniformly to apply bioactivity bacteria agent
Pending waters is added in, wherein, bioactivity bacteria agent is with bacillus subtilis（Bacillus subtilis）, Amur it is false
Monad（Pseudomonas stutzeri）, candida aquatica（Candida aquatica）For strain, use is organic, nothing
Machine raw material, is formulated compound after each strain individually fermentation.Step 2, be monitored pending waters, when 7-8 is small after, then
Apply chlorella solution by spraying machinery in pending waters, wherein, the chlorella content of chlorella solution is 3~8 × 107
Cfu/ml, pH value are 6.5-9.5.Step 3, to spray chlorella solution waters be monitored, when 6-7 is small after, according to monitoring
The cyanobacteria state of detection, repeat step 1, the processing step of step 2.
The technical program, the bioactivity bacteria agent in the step 1, effective total viable count（Containing bacillus subtilis,
Pseudomonas stutzeri, candida aquatica）≥2×108 A/mL, bacillus number >=1 × 108A/mL, wherein, it is effectively living
Bacterium is containing bacillus subtilis, Pseudomonas stutzeri, candida aquatica.
The technical program, in the step 3, before low temperature drying, uniformly sprayed on the cereal for treat low temperature drying vaporific
Water, sprinkling water temperature is 10 degrees Celsius -12 degrees Celsius, then natural air drying -60 minutes 30 minutes in warehouse.
The technical program, the pH of bioactivity bacteria agent is 4-6.5 in the step 1, the sum of mould miscellaneous bacteria for≤
3.0×105 Cfu/ml, coliform are≤10 MPN/100mL；In the step 1 bioactivity bacteria agent spray when, it is necessary to
The living bacteria count of microbial inoculum is measured, is comprised the following steps, the sample 25mL fully mixed, be put into sterile working by a
1 is made in sterilizing conical flask containing 225mL sterile biological brine：10 uniform dilution.B, 1 is drawn with 1mL sterilized straws：
10 dilution 1mL, inject in the test tube containing 9mL sterile salines, shaking mixes, and makes 1 slowly along tube wall：100
Dilution.C, 1mL sterilized straws separately are taken, by aforesaid operations order, does 10 times of incremental dilutions, be so often incremented by once, that is, change
With 1 1mL sterilized straw.D, 1 is selected：10-7、1：10-8Two acceptable diluent degree, respectively take 1mL to be separately added into counting culture
Base plate, each dilution factor make three plates.E, dilution is moved into plate, in time should be noted the counting culture medium for being cooled to 45 DEG C
Enter plate about 15mL-20mL, and rotate plate to make to be uniformly mixed, while be poured into culture medium is counted added with 1mL dilution samples
Make blank control in the sterilizing plates of sterile saline, bacillus subtilis, Pseudomonas stutzeri are trained using glucose
Base culture is supported, candida aquatica uses starch culture-medium culture.F, after agar completely solidification after, overturn tablet, put 32 DEG C ±
Culture 48h ± 2h in 1 DEG C of incubator, chooses tablet of the clump count between 30-300 and is counted.G, bacterium number calculates, according to every
Then one dilution factor is multiplied by the extension rate of sample, is obtained viable count in every milliliter of sample using the average of three flat-plate bacterial colonies；
If any two dilution factors, how the bacterium colony of its growth determines between 30-300 depending on ratio between two, as its ratio is less than 2,
It should report its average；Such as larger than 2, then report wherein less numeral.
The technical program, when bioactivity bacteria agent sprays in the step 1, it is necessary to the living bacteria count of microbial inoculum into
Row measure, comprises the following steps, and a, will first pour into 10 mm cuvettes for cultivating the fluid nutrient medium of strain, selects 600nm
Zero point is adjusted on wavelength spectrophotometer, as blank control.B, 300 mL of sample is put into sterilizing wide-mouth bottle again, up and down acutely
Shaking 30 times -40 times, is suctioned out in injection 10mm cuvettes with sterilized straw, measures the OD value under 600nm wavelength.
The technical program, the component of starch culture-medium is starch 20g, potassium nitrate 1.0g, phosphoric acid hydrogen two in the step 1
Potassium 0.5g, sodium chloride 0.5g, magnesium sulfate 0.5g, ferric sulfate 0.01g, after mixing plus distilled water is first to 1000mL, pH7.2 ± 0.1
First all of above component is added in distilled water, by 2% addition agar, dissolving is reheated, corrects pH 7.2 ± 0.1, finally divide
Dress, 121 DEG C, time 15min-20min of autoclaving, heating fusing agar, uses when being cooled to 45 DEG C during use.
The technical program, in the step 1 glucose cultivate the glucose 6g of base, ammonium chloride 1.0g, sodium chloride 1.0g,
1.0 g of dipotassium hydrogen phosphate, potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.2g, after mixing plus distilled water is to 1000mL, pH7.2 ± 0.1,
All of above component is added in distilled water first, by 2% addition agar, dissolving is reheated, corrects pH 7.2 ± 0.1, finally
Packing, 121 DEG C of autoclaving, time be 15min-20 min, during use heating melt agar, used when being cooled to 45 DEG C.
The technical program, it is necessary to be carried out to the mould miscellaneous bacteria of microbial inoculum when bioactivity bacteria agent sprays in the step 1
Measure, is tested, composition is potassium dihydrogen phosphate 1.0g, glucose 10.0g, first adds all of above composition using Martin's culture medium
Enter in distilled water, then add rose-bengal aqueous solution, separately dissolve chloramphenicol with a small amount of ethanol, add in culture medium and dissolve by heating, most
Packing, 121 DEG C of autoclaving, time be 15min-20 min afterwards, during use heating melt agar, used when being cooled to 45 DEG C.
The technical program, it is necessary to chlorella content in chlorella solution when spraying chlorella solution in the step 2
Tested, comprised the following steps, a, microscopy counting chamber, before sample-adding, first carry out microscopy, observation is counted to the counting chamber of tally
Answer no-sundries in room.B, be loaded, dry blood counting chamber covered will be cleaned, with sterile pipette by bead algae solution by
Coverslip edge drips a droplet, allows algae solution to be advanced into counting chamber certainly by capillary osmosis along gap, can not there is bubble generation.c、
Microscopic counting, after standing 5min, blood counting chamber is placed on microscope carrier, first finds counting chamber with low power lens,
It is converted into high power lens to be counted, counting chamber is selected 5 middle lattice and counted.D, calculate, count the number of chlorella in lattice in 5
Measure A, then in algae solution chlorella quantity=A/80 × 100 × 10000 × extension rate/mL, wherein, blood counting chamber for 25 ×
16, counting chamber thickness is 0.1mm.E, clear up, it is used during blood counting chamber, beaker, test tube, pipette, microscope etc. are tested
To article clean out.F, the measure of pH values, takes and pours into right amount in beaker, and pH is directly measured with the acidometer of calibrated mistake
Value.
The technical program, it is necessary to carry out fresh water culture when the chlorella content in the chlorella solution is tested
Base, wherein, cultivate reagent component is NaNO3, K2HPO4, MgSO47H2O, CaCl22H2O, citric acid, ferric citrate
Or ironic citrate, Na2EDTA, Na2CO3, A5, wherein, it is each form content be respectively 1.5000g/L, 0.0400g/L,
0.0750g/L, 0.0360 g/L, 0.0060g/L, 0.006g/L or 0.004g/L, 0.0010g/L, 0.0200g/L, 1.0mL；
The A5 by ZnSO47H2O, CuSO45H2O, MoO3, H3BO3, MnCl24H2O and content be respectively 0.222g/L,
0.079g/L、0.015g/L、2.86g/L、1.81g/L。
The technical program, it is necessary to carry out cultivation in sea water when the chlorella content in the chlorella solution is tested
Base, wherein, be made of liquid A and liquid B, solution A agent formulations for FeCl36H2O, MnCl24H2O, H3BO3, Na2EDTA,
NaH2PO42H2O, NaNO3 and content are respectively 6.5g, 1.8g, 168g, 225g, 100g, 500g, and second liquid agent formulations are
ZnCl2, CoCl26H2O, (NH4) 6Mo7O244H2O, CuSO45H2O and content be respectively 2.1g, 2.0g, 0.9g,
2.0g, preparation method is first to be dissolved in solution A in 5L pure water by content sequence, then second liquid is dissolved in 100ml by content sequence
In pure water, then solution A and second liquid sterilized, then by the liquid A and liquid B after sterilizing, solution A：Second liquid=1000:1 or 500：1.
Compared with prior art, it is of the invention a kind of to exist applied to the prevention of water body cyanobacteria and the beneficial effect of suppressing method
In：Pass through the bioactivity bacteria agent of high-content（Bacillus content 10,000,000,000）, energy fast decoupled is applied organic in waters
Matter, while application is supplemented beneficial to chlorella solution, beneficial bacterium is quickly absorbed the nutriment in applied waters, so that quick numerous
Grow, harmful algae living space is reduced high-content bacillus and decompose organic matter, be also adjusted in synchronism and apply nitrogen in waters, phosphorus ratio
Example, it is final so as to apply N∶P ratio in waters normal, so that effectively decrease cyanobacteria produces by promoting nitrogen with phosphorus, with nitrogen consolidate phosphorus
Condition, realize efficiently prevent and administer purpose.
Embodiment
With reference to specific embodiment, the present invention is furture elucidated.
Embodiment
The present invention provides a kind of prevention and suppressing method applied to water body cyanobacteria, comprises the following steps, step 1, first
Determine the waters of application bioactivity bacteria agent product, recycle sprinkling machinery to be uniformly applied to bioactivity bacteria agent and waits to locate
Waters is managed, wherein, bioactivity bacteria agent is with bacillus subtilis（Bacillus subtilis）, Pseudomonas stutzeri
（Pseudomonas stutzeri）, candida aquatica（Candida aquatica）For strain, using organic and inorganic original
Material, is formulated compound after each strain individually fermentation.Step 2, be monitored pending waters, when 7-8 is small after, then treating
Handle waters and apply chlorella solution by spraying machinery, wherein, the chlorella content of chlorella solution is 3~8 × 107
Cfu/ml, pH value are 6.5-9.5.Step 3, to spray chlorella solution waters be monitored, when 6-7 is small after, according to monitoring
The cyanobacteria state of detection, repeat step 1, the processing step of step 2.
It is further preferred that the bioactivity bacteria agent in the step 1, effective total viable count（Containing bacillus subtilis,
Pseudomonas stutzeri, candida aquatica）≥2×108 A/mL, bacillus number >=1 × 108A/mL, wherein, it is effectively living
Bacterium is containing bacillus subtilis, Pseudomonas stutzeri, candida aquatica；And in the step 3, before low temperature drying, treating low temperature
Spray water is uniformly sprayed on the cereal of drying, sprinkling water temperature is 10 degrees Celsius -12 degrees Celsius, then natural air drying 30 divides in warehouse
Clock -60 minutes；And the pH of bioactivity bacteria agent is 4-6.5 in the step 1, the sum of mould miscellaneous bacteria is≤3.0 × 105
Cfu/ml, coliform are≤10 MPN/100mL；, it is necessary to microbial inoculum when bioactivity bacteria agent sprays in the step 1
Living bacteria count is measured, and is comprised the following steps, and the sample 25mL fully mixed, be put into containing 225mL with sterile working by a
1 is made in the sterilizing conical flask of sterile biological brine：10 uniform dilution.B, 1 is drawn with 1mL sterilized straws：10 dilutions
1mL, injects in the test tube containing 9mL sterile salines, shaking mixes, and makes 1 slowly along tube wall：100 dilution.c、
1mL sterilized straws separately are taken, by aforesaid operations order, 10 times of incremental dilutions is done, is so often incremented by once, that is, uses 1 1mL instead
Sterilized straw.D, 1 is selected：10-7、1：10-8Two acceptable diluent degree, respectively take 1mL to be separately added into and count culture medium plate, often
A dilution factor makees three plates.E, dilution is moved into plate, should will be cooled to 45 DEG C of counting culture medium injection plate about in time
15mL-20mL, and rotate plate and make to be uniformly mixed, while culture medium will be counted and be poured into sterilizing added with 1mL dilution samples
Making blank control in the sterilizing plates of physiological saline, bacillus subtilis, Pseudomonas stutzeri use dextrose culture-medium culture,
Candida aquatica uses starch culture-medium culture.F, after agar completely solidification, tablet is overturn, is put in 32 DEG C of ± 1 DEG C of incubators
48h ± 2h is cultivated, tablet of the clump count between 30-300 is chosen and is counted.G, bacterium number calculates, and is adopted according to each dilution factor
With the average of three flat-plate bacterial colonies, the extension rate of sample is then multiplied by, obtains viable count in every milliliter of sample；It is dilute if any two
How degree of releasing, the bacterium colony of its growth to determine between 30-300 depending on ratio between two, as its ratio is less than 2, should report that it is flat
Mean；Such as larger than 2, then report wherein less numeral.
The technical program, when bioactivity bacteria agent sprays in the step 1, it is necessary to the living bacteria count of microbial inoculum into
Row measure, principle are to form the microorganism of bacterium colony because of its growth so that the turbidity of liquid culture increases, the sample of bacteria suspension containing bacterium
The concentration of product is inversely proportional with light transmittance within the specific limits, with OD value（OD values）It is directly proportional, utilize ultraviolet specrophotometer
（Photoelectric colorimetry）The OD values of the sample bacteria suspension under certain wavelength are measured, show the bacteria containing amount of sample indirectly, including it is following
Step, a, will first pour into 10 mm cuvettes for cultivating the fluid nutrient medium of strain, select 600nm wavelength spectrophotometers
Upper adjusting zero point, as blank control.B, 300 mL of sample is put into sterilizing wide-mouth bottle again, up and down acutely shaking 30 times -40 times,
Suctioned out with sterilized straw in injection 10mm cuvettes, measure the OD value under 600nm wavelength.
The prevention applied to water body cyanobacteria of the present invention and suppressing method, the component of starch culture-medium is in the step 1
Starch 20g, potassium nitrate 1.0g, dipotassium hydrogen phosphate 0.5g, sodium chloride 0.5g, magnesium sulfate 0.5g, ferric sulfate 0.01g, add after mixing
To 1000mL, pH7.2 ± 0.1, first adds all of above component in distilled water distilled water, by 2% addition agar, reheats
Dissolving, corrects pH 7.2 ± 0.1, finally packing, 121 DEG C, time 15min-20min of autoclaving, heating fusing during use
Agar, uses when being cooled to 45 DEG C；And glucose cultivates glucose 6g, ammonium chloride 1.0g, the sodium chloride of base in the step 1
1.0g, 1.0 g of dipotassium hydrogen phosphate, potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.2g, after mixing plus distilled water is to 1000mL, and pH7.2 ±
0.1, all of above component is added in distilled water first, by 2% addition agar, dissolving is reheated, corrects pH 7.2 ± 0.1,
Finally dispense, 121 DEG C of autoclaving, the time is 15min-20 min, and heating fusing agar during use, makes when being cooled to 45 DEG C
With；And in the step 1 when bioactivity bacteria agent sprays, it is necessary to be measured to the mould miscellaneous bacteria of microbial inoculum, trained using Martin
Base test is supported, composition is potassium dihydrogen phosphate 1.0g, glucose 10.0g, is first added all of above composition in distilled water, then add
Rose-bengal aqueous solution, separately dissolves chloramphenicol with a small amount of ethanol, adds in culture medium and dissolves by heating, finally packing, autoclaving
121 DEG C, the time is 15min-20 min, and heating fusing agar during use, uses when being cooled to 45 DEG C.
The prevention applied to water body cyanobacteria of the present invention and suppressing method, when spraying chlorella solution in the step 2, need
Want the chlorella content in chlorella solution to be tested, comprise the following steps, a, microscopy counting chamber, before sample-adding, first to counting
The counting chamber of plate carries out microscopy, and observation counting chamber answers no-sundries.B, it is loaded, dry blood counting chamber will be cleaned and closed the lid glass
Piece, drips a droplet by coverslip edge by bead algae solution with sterile pipette, allows algae solution to lean on capillary osmosis certainly along gap
Counting chamber is advanced into, there can not be bubble generation.C, microscopic counting, after standing 5min, microscope is placed by blood counting chamber
On objective table, counting chamber first is found with low power lens, high power lens is converted into and is counted, counting chamber is selected 5 middle lattice and counted
Number.D, calculate, count the quantity A of chlorella in lattice in 5, then in algae solution chlorella quantity=A/80 × 100 × 10000 × dilute
Multiple/mL is released, wherein, blood counting chamber is 25 × 16, and counting chamber thickness is 0.1mm.E, clear up, by blood counting chamber, burn
Used article is cleaned out in the experiment such as cup, test tube, pipette, microscope.F, the measure of pH values, takes and pours into burning in right amount
In cup, pH values are directly measured with the acidometer of calibrated mistake.
The prevention applied to water body cyanobacteria of the present invention and suppressing method, chlorella content in the chlorella solution into
, it is necessary to carry out fresh water culture medium during row test（Method one）, wherein, cultivate reagent component is NaNO3、K2HPO4、MgSO4·
7H2O, CaCl22H2O, citric acid, ferric citrate or ironic citrate, Na2EDTA, Na2CO3, A5, wherein, each composition contains
Amount is respectively 1.5000g/L, 0.0400g/L, 0.0750g/L, 0.0360 g/L, 0.0060g/L, 0.006g/L or 0.004g/
L、0.0010g/L、0.0200g/L、1.0mL；The A5 by ZnSO47H2O, CuSO45H2O, MoO3, H3BO3,
MnCl24H2O and content are respectively 0.222g/L, 0.079g/L, 0.015g/L, 2.86g/L, 1.81g/L；And the bead
, it is necessary to carry out sea water medium when chlorella content in algae solution is tested, wherein, it is made of liquid A and liquid B, solution A
Agent formulations are distinguished for FeCl36H2O, MnCl24H2O, H3BO3, Na2EDTA, NaH2PO42H2O, NaNO3 and content
For 6.5g, 1.8g, 168g, 225g, 100g, 500g, second liquid agent formulations are ZnCl2, CoCl26H2O, (NH4)
6Mo7O244H2O, CuSO45H2O and content are respectively 2.1g, 2.0g, 0.9g, 2.0g, and preparation method is first by solution A
It is dissolved in by content sequence in 5L pure water, then second liquid is dissolved in 100ml pure water by content sequence, then solution A and second liquid is gone out
Bacterium, then by the liquid A and liquid B after sterilizing, solution A：Second liquid=1000:1 or 500：1（Room temperature is kept in dark place, and placing 3~4 weeks makes
With）.
The prevention applied to water body cyanobacteria of the present invention and suppressing method, chlorella content in the chlorella solution into
, it is necessary to carry out fresh water culture medium during row test（Method two）, wherein, cultivate reagent component is NaNO3、K2HPO4、MgSO4·
7H2O, CaCl22H2O, citric acid, ferric citrate or ironic citrate, Na2EDTA, Na2CO3, A5, wherein, each composition contains
Amount is respectively 1.5000g/L, 0.0400g/L, 0.0750g/L, 0.0360 g/L, 0.0060g/L, 0.006g/L or 0.004g/
L、0.0010g/L、0.0200g/L、1.0mL；The A5 by ZnSO47H2O, CuSO45H2O, Na2MoO4, H3BO3,
MnCl24H2O and content are respectively 0.222g/L, 0.079g/L, 0.21 g/L, 2.86g/L, 1.81g/.
The prevention applied to water body cyanobacteria of the present invention and suppressing method, chlorella content in the chlorella solution into
, it is necessary to carry out fresh water culture medium during row test（Method three）, wherein, cultivate reagent component is NaNO3、K2HPO4、MgSO4·
7H2O, CaCl22H2O, citric acid, ferric citrate or ironic citrate, Na2EDTA, Na2CO3, A5, wherein, each composition contains
Amount is respectively 1.5000g/L, 0.0400g/L, 0.0750g/L, 0.0360 g/L, 0.0060g/L, 0.006g/L or 0.004g/
L、0.0010g/L、0.0200g/L、1.0mL；The A5 by ZnSO47H2O, CuSO45H2O, Na2MoO2H2O,
H3BO3, MnCl24H2O and content are respectively 0.222g/L, 0.079g/L, 0.39g/L, 2.86g/L, 1.81g/.
The prevention applied to water body cyanobacteria of the present invention and suppressing method, pass through the bioactivity bacteria agent of high-content（Bud
Spore bacillus content 10,000,000,000）, energy fast decoupled applies the organic matter in waters, while supplements application beneficial to chlorella solution, makes to have
Beneficial bacteria quickly absorbs the nutriment in applied waters, so as to quickly breed, harmful algae living space is reduced high-content bud
Spore bacillus decomposes organic matter, is also adjusted in synchronism and applies nitrogen in waters, phosphorus ratio, final to cause by promoting nitrogen with phosphorus, consolidating phosphorus with nitrogen
N∶P ratio is normal in applied waters, so as to effectively weaken the condition of cyanobacteria production, realizes the purpose efficiently prevented and administered.
The prevention applied to water body cyanobacteria of the present invention and suppressing method, its can also according to needing to use by the following method,
Apply within first day bioactivity bacteria agent, 2 mu apply one bag（1000 grams/bag）, apply within second day chlorella solution, 5 mu of applications
One bottle（1000ml/ bottles）, applied once per 10-15 days.
The present invention applied to the prevention of water body cyanobacteria and the strain morphological feature of suppressing method, bacillus subtilis
Bacterium（Bacillus subtilis）, 40 × 10 times of hypothalluses of microscope are in rod-shaped, 2 μm -3 μm of length, 0.7 μm wide -
0.8 μm, gemma is column or ellipse, and middle life or near middle raw, has motility, Gram's staining is the positive, in agar plate
On bacterium colony it is circular, have fold sometimes in yellow-white or shallow white, neat in edge, surface；Pseudomonas stutzeri
（Pseudomonas stutzeri）, 40 × 10 times of hypothalluses of microscope are rod-shaped, can produce short side under certain condition
Raw flagellum, Gram's staining are feminine gender, and the bacterium colony on agar plate is circular, in yellowish white or yellow-white, neat in edge, table
Face is relatively dry；Candida aquatica（Candida aquatica）, 40 × 10 times of hypothallus forms of microscope are in rod-shaped, can be produced
Mycelium is given birth to, the bacterium colony on agar plate is smooth, protuberance, circular, white or canescence, and neat in edge, surface is in sometimes
Mucus shape.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, some improvement can also be made, these improvement also should be regarded as the present invention's
Protection domain.
Claims (10)
- A kind of 1. prevention and suppressing method applied to water body cyanobacteria, it is characterised in that：Comprise the following steps,Step 1, determine to apply the waters of bioactivity bacteria agent product first, recycles sprinkling machinery by bioactivity bacteria agent Pending waters is uniformly applied to, wherein, bioactivity bacteria agent is with bacillus subtilis（Bacillus subtilis）、 Pseudomonas stutzeri（Pseudomonas stutzeri）, candida aquatica（Candida aquatica）For strain, use Organic and inorganic raw material, is formulated compound after each strain individually fermentation；Step 2, be monitored pending waters, when 7-8 is small after, then pending waters pass through spray machinery apply chlorella Solution, wherein, the chlorella content of chlorella solution is 3~8 × 107Cfu/ml, pH value are 6.5-9.5；Step 3, to spray chlorella solution waters be monitored, when 6-7 is small after, according to monitor and detection cyanobacteria state, weight Multiple step 1, the processing step of step 2.
- A kind of 2. prevention and suppressing method applied to water body cyanobacteria according to claim 1, it is characterised in that：The step Bioactivity bacteria agent in rapid 1, effective total viable count（Containing bacillus subtilis, Pseudomonas stutzeri, candida aquatica） ≥2×108 A/mL, bacillus number >=1 × 108A/mL, wherein, effective viable bacteria is containing bacillus subtilis, Amur vacation unit cell Bacterium, candida aquatica.
- A kind of 3. prevention and suppressing method applied to water body cyanobacteria according to claim 1 or 2, it is characterised in that：Institute The pH for stating bioactivity bacteria agent in step 1 is 4-6.5, and the sum of mould miscellaneous bacteria is≤3.0 × 105 Cfu/ml, coliform For≤10 MPN/100mL；, it is necessary to be surveyed to the living bacteria count of microbial inoculum when bioactivity bacteria agent sprays in the step 1 It is fixed, comprise the following steps,A, the sample 25mL fully mixed is put into the sterilizing conical flask containing 225mL sterile biological brine with sterile working and made Into 1：10 uniform dilution；B, 1 is drawn with 1mL sterilized straws：10 dilution 1mL, inject the test tube containing 9mL sterile salines slowly along tube wall Interior, shaking mixes, and makes 1：100 dilution；C, 1mL sterilized straws separately are taken, by aforesaid operations order, does 10 times of incremental dilutions, be so often incremented by once, that is, use 1 instead Branch 1mL sterilized straws；D, 1 is selected：10-7、1：10-8Two acceptable diluent degree, respectively take 1mL to be separately added into and count culture medium plate, each dilution Degree makees three plates；E, dilution is moved into plate, the counting culture medium for being cooled to 45 DEG C should be injected plate about 15mL-20mL in time, and rotate Plate makes to be uniformly mixed, while is put down the sterilizing that culture medium is poured into added with the sterile saline of 1mL dilution samples is counted Make blank control in ware, bacillus subtilis, Pseudomonas stutzeri use dextrose culture-medium culture, and candida aquatica uses Starch culture-medium culture；F, after agar completely solidification, tablet is overturn, puts culture 48h ± 2h in 32 DEG C of ± 1 DEG C of incubators, clump count is chosen and exists Tablet between 30-300 is counted；G, bacterium number calculates, the average according to each dilution factor using three flat-plate bacterial colonies, is then multiplied by the extension rate of sample, Obtain viable count in every milliliter of sample；If any two dilution factors, the bacterium colony of its growth between 30-300, depending on ratio between two how To determine, as its ratio be less than 2, should report its average；Such as larger than 2, then report wherein less numeral.
- A kind of 4. prevention and suppressing method applied to water body cyanobacteria according to claim 1, it is characterised in that：The step , it is necessary to be measured to the living bacteria count of microbial inoculum when bioactivity bacteria agent sprays in rapid 1, comprise the following steps,A, it will first pour into 10 mm cuvettes, selected on 600nm wavelength spectrophotometers for cultivating the fluid nutrient medium of strain Zero point is adjusted, as blank control；B, 300 mL of sample is put into sterilizing wide-mouth bottle again, acutely shaking 30 times -40 times, are suctioned out with sterilized straw and injected up and down In 10mm cuvettes, the OD value under 600nm wavelength is measured.
- A kind of 5. prevention and suppressing method applied to water body cyanobacteria according to claim 3, it is characterised in that：The step The component of starch culture-medium is starch 20g, potassium nitrate 1.0g, dipotassium hydrogen phosphate 0.5g, sodium chloride 0.5g, magnesium sulfate in rapid 1 0.5g, ferric sulfate 0.01g, after mixing plus distilled water is to 1000mL, and pH7.2 ± 0.1, all of above component is added distill first In water, by 2% addition agar, dissolving is reheated, corrects pH 7.2 ± 0.1, finally packing, 121 DEG C of autoclaving, the time is 15min-20min, heating fusing agar, uses when being cooled to 45 DEG C during use.
- A kind of 6. prevention and suppressing method applied to water body cyanobacteria according to claim 3, it is characterised in that：The step Glucose cultivates glucose 6g, ammonium chloride 1.0g, sodium chloride 1.0g, 1.0 g of dipotassium hydrogen phosphate, the potassium dihydrogen phosphate of base in rapid 1 1.0g, magnesium sulfate 0.2g, after mixing plus distilled water is to 1000mL, and pH7.2 ± 0.1, all of above component is added distill first In water, by 2% addition agar, dissolving is reheated, pH 7.2 ± 0.1 is corrected, finally dispenses, 121 DEG C of autoclaving, the time is 15min-20 min, heating fusing agar, uses when being cooled to 45 DEG C during use.
- 7. according to a kind of prevention and suppressing method applied to water body cyanobacteria described in claim 1, it is characterised in that：The step , it is necessary to be measured to the mould miscellaneous bacteria of microbial inoculum when bioactivity bacteria agent sprays in 1, tested using Martin's culture medium, composition For potassium dihydrogen phosphate 1.0g, glucose 10.0g, all of above composition is added in distilled water first, then adds rose-bengal water-soluble Liquid, separately dissolves chloramphenicol with a small amount of ethanol, adds in culture medium and dissolves by heating, finally packing, 121 DEG C of autoclaving, and the time is 15min-20 min, heating fusing agar, uses when being cooled to 45 DEG C during use.
- 8. according to a kind of prevention and suppressing method applied to water body cyanobacteria described in claim 1, it is characterised in that：The step , it is necessary to the chlorella content in chlorella solution is tested when spraying chlorella solution in 2, comprise the following steps,A, microscopy counting chamber, before sample-adding, first carries out microscopy, observation counting chamber answers no-sundries to the counting chamber of tally；B, it is loaded, dry blood counting chamber covered will be cleaned, with sterile pipette by bead algae solution by coverslip Edge drips a droplet, allows algae solution to be advanced into counting chamber certainly by capillary osmosis along gap, can not there is bubble generation；C, microscopic counting, after standing 5min, blood counting chamber is placed on microscope carrier, first uses low power lensCounting chamber is found, high power lens is converted into and is counted, counting chamber is selected 5 middle lattice and counted；D, calculate, count the quantity A of chlorella in lattice in 5, then in algae solution chlorella quantity=A/80 × 100 × 10000 × dilute Multiple/mL is released, wherein, blood counting chamber is 25 × 16, and counting chamber thickness is 0.1mm；E, clear up, used article is cleaned out during blood counting chamber, beaker, test tube, pipette, microscope etc. are tested；F, the measure of pH values, takes and pours into right amount in beaker, and pH values are directly measured with the acidometer of calibrated mistake.
- 9. according to a kind of prevention and suppressing method applied to water body cyanobacteria described in claim 8, it is characterised in that：The bead , it is necessary to carry out fresh water culture medium when chlorella content in algae solution is tested, wherein, cultivate reagent component is NaNO3、 K2HPO4, MgSO47H2O, CaCl22H2O, citric acid, ferric citrate or ironic citrate, Na2EDTA, Na2CO3, A5, Wherein, respectively composition content be respectively 1.5000g/L, 0.0400g/L, 0.0750g/L, 0.0360 g/L, 0.0060g/L, 0.006g/L or 0.004g/L, 0.0010g/L, 0.0200g/L, 1.0mL；The A5 is by ZnSO47H2O, CuSO4 5H2O, MoO3, H3BO3, MnCl24H2O and content be respectively 0.222g/L, 0.079g/L, 0.015g/L, 2.86g/L, 1.81g/L。
- 10. according to a kind of prevention and suppressing method applied to water body cyanobacteria described in claim 8, it is characterised in that：It is described small , it is necessary to carry out sea water medium when chlorella content in ball algae solution is tested, wherein, it is made of liquid A and liquid B, first Liquid agent formulations are FeCl36H2O, MnCl24H2O, H3BO3, Na2EDTA, NaH2PO42H2O, NaNO3 and content point Not Wei 6.5g, 1.8g, 168g, 225g, 100g, 500g, second liquid agent formulations for ZnCl2, CoCl26H2O, (NH4) 6Mo7O244H2O, CuSO45H2O and content are respectively 2.1g, 2.0g, 0.9g, 2.0g, and preparation method is first by solution A It is dissolved in by content sequence in 5L pure water, then second liquid is dissolved in 100ml pure water by content sequence, then solution A and second liquid is gone out Bacterium, then by the liquid A and liquid B after sterilizing, solution A：Second liquid=1000:1 or 500：1.
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| CN201711334524.4A CN107986456A (en) | 2017-12-14 | 2017-12-14 | A kind of prevention and suppressing method applied to water body cyanobacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109593681A (en) * | 2018-12-28 | 2019-04-09 | 天津坤禾生物科技集团股份有限公司 | A kind of composite bacteria agent and its preparation method and application for preventing and treating aquaculture cyanobacterial bloom |
| CN114958638A (en) * | 2020-11-17 | 2022-08-30 | 江苏省农业科学院 | Composite microbial inoculum for reducing microcystin content in aquatic products and use method thereof |
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| CN109593681A (en) * | 2018-12-28 | 2019-04-09 | 天津坤禾生物科技集团股份有限公司 | A kind of composite bacteria agent and its preparation method and application for preventing and treating aquaculture cyanobacterial bloom |
| CN114958638A (en) * | 2020-11-17 | 2022-08-30 | 江苏省农业科学院 | Composite microbial inoculum for reducing microcystin content in aquatic products and use method thereof |
| CN114958638B (en) * | 2020-11-17 | 2024-04-23 | 江苏省农业科学院 | Composite microbial agent for reducing microcystin content in aquatic products and application method thereof |
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