CN107950536A

CN107950536A - A kind of bactericidal composition containing Bravo

Info

Publication number: CN107950536A
Application number: CN201711258125.4A
Authority: CN
Inventors: 刘志伟
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-12-04
Filing date: 2017-12-04
Publication date: 2018-04-24

Abstract

The invention discloses a kind of bactericidal composition containing Bravo, Bravo and isoniazid, the ratio between parts by weight of Bravo and isoniazid are 12:1‑11:1.The present invention is 12 particularly in the ratio between parts by weight of Bravo and isoniazid by the way that Bravo and isoniazid compatibility to be found to have the function that collaboration prevention leaf spot well under specific ratio:1‑11:Between 1, its synergy is apparent.

Description

A kind of bactericidal composition containing Bravo

Technical field

The present invention relates to a kind of medicament, is specifically a kind of bactericidal composition containing Bravo, belongs to agricultural technology field.

Background technology

Bravo is wide spectrum, protective fungicide.The mechanism of action is can be with the glyceraldehyde 3-phosphate dehydrogenation in fungal cell Enzyme is had an effect, and is combined with the protein containing cysteine in the enzyme, so as to destroy the enzymatic activity, makes that fungal cell's is new Old metabolism is damaged and loses vitality.Bravo does not have uptake and translocation effect, but after being sprayed onto on plant, can be on body surface There is good tackness, be not easy to be fallen by rain drop erosion, therefore the drug effect phase is longer.

Isoniazid invention has mycobacterium tuberculosis high selectivity, bactericidal effect is strong, and people are to isoniazid in nineteen fifty-two Although mechanism of action done detailed research, it is also not fully aware of.A kind of saying is that isoniazid is converted into isonicotinic acid, different Antimetabolite of the nicotinic acid as nicotinic acid, NAD+ is attached to instead of nicotinic acid, and the NAD+ cheated cannot then be catalyzed normal oxidation Reduction reaction.Another saying is the effect by blocking desaturase, and isoniazid suppresses the conversion of C24 and C26 saturated fatty acids To C24 and C26 unrighted acids, and these unrighted acids are particularly likely that the precursor of mycolic acid, and mycolic acid is then thin A kind of key component of bacterium cell membrane, suppresses the biosynthesis of mycolic acid, then loses the acid resistance of bacterium, this mechanism is abundant Illustrate the selectivity that isoniazid acts on the cell membrane of tubercle bacillus.

Actual pesticide experience have shown that, repeat and single-minded apply a kind of reactive compound to prevent harmful fungoid very It will cause the fast selective of fungal bacterial strain in the case of more, to reduce the danger of refractory fungal bacterial strain selectivity, at present usually Harmful fungoid is prevented using the mixture of different activities compound.By by the reactive compound with the different mechanisms of action into Row combination, can delaying drug resistance produce, reduce amount of application, reduce cost accounting.It is found by the applicant that Bravo is answered with isoniazid Match somebody with somebody, there is obvious synergistic effect.

The content of the invention

In view of the above-mentioned problems of the prior art, it is an object of the invention to provide the Bactericidal composite containing Bravo Thing, it is to be compounded the sterilization component of two kinds of different structures, the different mechanisms of action, is to expand prevention spectrum and raising drug effect Effective way, it is often more important that avoid or delay generation of the disease to new composition resistance.To achieve the object of the present invention, inventor Bravo and isoniazid are compounded, for preventing the disease of crop.

To achieve these goals, the technical solution adopted by the present invention is：One kind includes two kinds of principle active components：Hundred bacterium Clearly and isoniazid.

The ratio between the Bravo and the parts by weight of isoniazid are 7:1-6:1.

The sum of the Bravo and the weight of isoniazid account for the 1-6% of herbicide gross weight.

Further include pure water, methyl oleate polyoxyethylene (30) dimerization hydroxy stearic acid fat.

The present invention under specific ratio by having found Bravo and isoniazid compatibility to have collaboration well to prevent leaf The effect of pinta, is 12 particularly in the ratio between parts by weight of Bravo and isoniazid:1-11:Between 1, it cooperates with prevention tikka Sick effect is apparent, and whole reagent cost is relatively low, and toxicity is smaller.

Embodiment：

Embodiment 1：

The ratio between parts by weight of Bravo and isoniazid are 15:1, the sum of weight of the Bravo and isoniazid accounts for The 2% of herbicide gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Embodiment 2：

The ratio between parts by weight of Bravo and isoniazid are 12:1, the sum of weight of the Bravo and isoniazid accounts for The 2% of herbicide gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus are pure water

Embodiment 3：

The ratio between parts by weight of Bravo and isoniazid are 11:1, the sum of weight of the Bravo and isoniazid accounts for The 2% of herbicide gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Embodiment 4：

The ratio between parts by weight of Bravo and isoniazid are 8:1, the sum of weight of the Bravo and isoniazid, which accounts for, to be removed The 2% of careless agent gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Embodiment 5：

The ratio between parts by weight of Bravo and isoniazid are 5:1, the sum of weight of the Bravo and isoniazid, which accounts for, to be removed The 2% of careless agent gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Embodiment 6：

The ratio between parts by weight of Bravo and isoniazid are 2:1, the sum of weight of the Bravo and isoniazid, which accounts for, to be removed The 2% of careless agent gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Embodiment 7：

The ratio between parts by weight of Bravo and isoniazid are 1:1, the sum of weight of the Bravo and isoniazid, which accounts for, to be removed The 2% of careless agent gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Embodiment 8：

The ratio between parts by weight of Bravo and isoniazid are 1:2, the sum of weight of the Bravo and isoniazid, which accounts for, to be removed The 2% of careless agent gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Embodiment 9：

The ratio between parts by weight of Bravo and isoniazid are 1:6, the sum of weight of the Bravo and isoniazid, which accounts for, to be removed The 2% of careless agent gross weight, methyl oleate 1%, polyoxyethylene (30) dimerization hydroxy stearic acid fat 1%, surplus is pure water.

Laboratory test：

Subjects：Leaf spot germ

According to the incidence of the experiment grade scale investigation whole strain blade of sponge gourd, disease index and prevention effect are calculated.Will Prevention effect is converted into niqueMin (y), and liquid is done height (μ g/ml) and is converted into numerical value (x), and virulence is calculated with least square method Concentration EC50 in equation and suppression, the toxicity index level co-toxicity coefficient (CTC) of medicament is calculated according to the abundant methods of Sun Yun.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

Percentage composition+B medicament toxicity index * the mixtures of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixtures The percentage composition of middle B

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] * 100CTC≤ 80, composition shows as antagonism, 80 ＜ CTC ＜ 120, and composition shows as summation action, CTC >=120, composition performance For synergistic effect.

Table 1 is Bravo：Isoniazid and its different ratio compounding are to leaf spot toxicity test interpretation of result.

From the measurement result of table 1, work as Bravo：Isoniazid is matched 12:1-11:When between 1, co-toxicity coefficient is big It is big to be higher than 190, there is obvious synergistic effect, and other ratios mix, co-toxicity coefficient unobvious.

Field efficacy checking test：

Test method：At the initial stage of a disease, first time spraying is carried out immediately, and second of dispenser is carried out after 7 days, each handles 4 A cell, each 20 square meter of cell.10 days investigation statistics incidences, each 5 points of cell are random before medicine and after second of medicine Sampling, 5 plants of crops of every investigation, investigates percentage and classification that the lesion area in whole strain per blade accounts for blade area, calculates Disease index and prevention effect.

It is expected that preventive effect (%)=X+Y-XY/100 (wherein, X, Y are single dose preventive effect)

Grade scale：

0 grade：Disease-free spot；

1 grade：Leaf spot lesion is less than 5, and length is less than 1cm；

3 grades：Leaf spot lesion 6-10, part scab length is more than 1cm；

5 grades：Leaf spot lesion 11-25, part scab is linked to be piece, and lesion area accounts for the 10-22% of leaf area；

7 grades：Leaf spot lesion more than 26, scab are linked to be piece, and lesion area accounts for the 26-50% of leaf area；

9 grades：Scab is linked to be piece, and more than 50% or the full leaf that lesion area accounts for leaf area are withered.

The field control effectiveness test of embodiment and control group to leaf spot.

It can be seen that embodiment 1 has significant prevention leaf spot effect, it is seen that Bravo is with isoniazid in certain proportion Lower proportioning collaboration prevention leaf spot effect is apparent, and single Bravo has certain prevention effect, single is different Cigarette hydrazine ability is also ineffective.The ratio between parts by weight of Bravo and isoniazid are 11:1-12:Synergy is most notable when 1, And effect is not very notable during other proportions.

Claims

1. a kind of bactericidal composition containing Bravo, it is characterised in that include two kinds of principle active components：Bravo and different cigarette Hydrazine.

2. the weight of a kind of bactericidal composition containing Bravo according to claim 1, the Bravo and isoniazid The ratio between number is 20:1-1:1.

A kind of 3. bactericidal composition containing Bravo according to claim 1, it is characterised in that the Bravo with it is different The sum of weight of cigarette hydrazine accounts for the 1-6% of herbicide gross weight.

4. a kind of bactericidal composition containing Bravo according to claim 1, it is characterised in that further include pure water, oil Sour methyl esters polyoxyethylene (30) dimerization hydroxy stearic acid fat.