CN107903298A - A kind of liftable pressurization gravity column for protein purification - Google Patents
A kind of liftable pressurization gravity column for protein purification Download PDFInfo
- Publication number
- CN107903298A CN107903298A CN201711444980.4A CN201711444980A CN107903298A CN 107903298 A CN107903298 A CN 107903298A CN 201711444980 A CN201711444980 A CN 201711444980A CN 107903298 A CN107903298 A CN 107903298A
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- CN
- China
- Prior art keywords
- gravity column
- gravity
- column
- liftable
- protein purification
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- 230000005484 gravity Effects 0.000 title claims abstract description 126
- 238000001742 protein purification Methods 0.000 title claims abstract description 23
- 238000000746 purification Methods 0.000 claims abstract description 22
- 238000010586 diagram Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention discloses a kind of liftable pressurization gravity column for protein purification, includes gravity column holder, gravity column purification component and gravity column lifting unit, the gravity column holder includes a base and vertical with the base two rail bracketses；Two rail bracketses are located at the both ends of the base；The both ends of the gravity column purification component are connected on two rail bracketses respectively, and " H " type is formed between two rail bracketses；The gravity column lifting unit is equipped with two rail bracketses, the both ends of the gravity column purification component are connected by the rail brackets with the gravity column lifting unit；The gravity column purification component includes a gravity column fixed plate and multiple gravity columns with protein purification；The gravity column, which coordinates, is equipped with dismountable chock plug；The gravity column is connected with pressue device.The present invention provide it is a kind of it is easy to operation, save time, high efficiency, the gravity column automatically controlled.
Description
Technical field
The invention belongs to the technical field of protein purification equipment, more particularly to a kind of liftable for protein purification
Pressurize gravity column.
Background technology
For industrial production, traditional gravity column purification device is simply not proposed to purify substantial amounts of albumen, AKTA
Can be applied not only to a variety of chromatographic columns with protein purification instruments such as SCG, and can Real Time Observation elution profile, adjustment stream at any time
Speed.But this kind of protein purification instrument pipeline is more, longer, prepare longer with the time of balance, if sample size is less, damage
Mistake rate can be higher, and an instrument can only use a kind of purification column at the same time, and conventional efficient is low.Traditional gravity column is mainly used in
In terms of scientific research, compared with purifying instrument, have it is easy to operate, save the time, can Simultaneous purification be multiple or multiple proteins
Advantage.
With the development of science and technology, more and more people begin to focus on field of scientific study, all kinds of protein drugs are made with biology
The appearance of product be unable to do without sturdy rigorous development phase, and it is research staff as far as possible that this, which is just needed in technology and equipment aspect,
Facility is provided.It can not meet easy to operation, high efficiency in terms of albumen gravity column purification also at present, automate the device of feature,
It is an object of the invention to provide it is a kind of it is easy to operation, save time, high efficiency, the gravity column purification device that can be automatically controlled.
The content of the invention
In order to solve the problems in the prior art, the purpose of the present invention is to, there is provided one kind is easy to operation, saves the time, is permanent high
Effect, the gravity column of automatic controlled height.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of liftable for protein purification pressurizes gravity column, includes gravity column holder, gravity column purification component and again
Power column lifting unit, the gravity column holder include a base and vertical with the base two rail bracketses；Described in two
Rail brackets is located at the both ends of the base；The both ends of the gravity column purification component are connected in two rail bracketses respectively
On, and " H " type is formed between two rail bracketses；The gravity column lifting is equipped with two rail bracketses
Portion, the both ends of the gravity column purification component are connected by the rail brackets with the gravity column lifting unit；The gravity
Column purification component includes a gravity column fixed plate and multiple gravity columns with protein purification；The gravity column, which coordinates, to be set
There is dismountable chock plug；The gravity column is connected with pressue device.
Preferably, the rail brackets is in long strip type, and a liftway is additionally provided with the rail brackets, and the lifting is logical
Road is used for disposing the gravity column fixed plate.
Preferably, the gravity column is equipped with a switch valve；The pressue device by the switch valve removably with
The gravity column is connected.The pressue device is convenient to be dismantled, and is more advantageous to the cleaning of gravity column, is closed the switch valve, described
The effect of gravity column is equivalent to the gravity column of straight tube.
Preferably, the gravity column lifting unit include be respectively fixedly connected on two rail bracketses the first motor,
First screw and the sliding shoe being set on first screw, the both ends of the gravity column fixed plate connect be fixed with it is described
Sliding shoe.The structure of the gravity column lifting unit is simple, easy to manufacture and install, it can be achieved that automated control, reduces labor intensity；
The both ends of the gravity column fixed plate are equipped with the identical gravity column lifting unit, then the gravity column assembly uniform force, sliding
It is dynamic smooth.
Preferably, the gravity column fixed plate is equipped with the multiple and matched gravity column open slot of the gravity column；Institute
It is in U-typed to state gravity column open slot.U-shaped gravity column open slot in the gravity column fixed plate, easy to the gravity column
Pickup.
Preferably, the inner side of each gravity column open slot is equipped with a pair of of elastic bumps, and the elastic bumps are used to block
State gravity column in residence.
Preferably, the bottom of the base, which is also tiled, skid resistant course.When the skid resistant course reduces motor operational vibration
Degree.
Preferably, multiple fixed hole positions are additionally provided with the base, multiple fixed hole positions are used to consolidate the base
Determine on the stationary platform.The base is fixedly connected on the testing stand of work by the fixed hole position, avoids motor
Position deviation during operational vibration.
Compared with prior art, the beneficial effects of the invention are as follows：
1 while the carry out purification process to one or more protein, improve work efficiency；
2nd, the gravity column length time, pressurization improved flow velocity using flow velocity can be caused slack-off, strengthened work efficiency；
3rd, gravity column fixed plate both ends are equipped with motor, realize the automated control to gravity column fixed plate height, reduce artificial
Work degree；
4th, removably pressue device, easy to the cleaning of gravity column；
5th, apparatus structure is simple, facilitates fabrication and installation, cost low.
Brief description of the drawings
Fig. 1 is a kind of one of liftable pressurization gravity rod structure schematic diagram for protein purification of the present embodiment.
Fig. 2 is the scheme of installation of the pressurizing ball of the present embodiment.
Fig. 3 is the rail brackets left view schematic diagram of the present embodiment.
Fig. 4 is the schematic top plan view of the gravity column fixed plate of the present embodiment.
Fig. 5 is the two of a kind of liftable pressurization gravity rod structure schematic diagram for protein purification of the present embodiment.
In figure：1st, base；2nd, rail brackets；21st, liftway；3rd, the first motor；4th, the first screw；5th, sliding shoe；6、
Gravity column fixed plate；7th, gravity column；8th, gravity column open slot；9th, elastic bumps；10th, chock plug；11st, switch valve；12nd, pressurization dress
Put；13rd, skid resistant course；14th, fixed hole position.
Embodiment
Clear, complete description is carried out to the technical solution of various embodiments of the present invention below with reference to attached drawing, it is clear that retouched
The embodiment stated is only the part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiment of the present invention, sheet
Field those of ordinary skill obtained all other embodiment on the premise of creative work is not made, belongs to this hair
Bright protected scope.
In the description of the present invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to
Easy to describe the present invention and simplify description, rather than instruction or imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ",
" the 3rd " is only used for description purpose, and it is not intended that instruction or hint relative importance.
In the description of the present invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；Can
To be mechanical connection or be electrically connected；It can be directly connected, can also be indirectly connected by intermediary, Ke Yishi
Connection inside two elements.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this
Concrete meaning in invention.
The present invention is described in further detail below through specific implementation examples and in conjunction with the accompanying drawings.
Embodiment
As shown in Fig. 1 ~ 5, a kind of liftable for protein purification pressurizes gravity column, includes gravity column holder, again
Power column purification component and gravity column lifting unit, the gravity column holder include a base 1 and two vertical with the base 1
Rail brackets 2；The rail brackets 2 is in long strip type, and a liftway 21 is additionally provided with the rail brackets 2；Led described in two
Rail stent 2 is located at the both ends of the base 1；The both ends of the gravity column purification component are connected in two rail bracketses 2 respectively
On, and " H " type is formed between two rail bracketses 2；The gravity column lifting is equipped with two rail bracketses 2
Portion, the both ends of the gravity column purification component are connected by the rail brackets 2 with the gravity column lifting unit；The gravity
Column lifting unit includes the first motor 3 being respectively fixedly connected on two rail bracketses 2, the first screw 4 and is set in described
Sliding shoe 5 on first screw 4, the both ends of the gravity column fixed plate 6, which connect, is fixed with the sliding shoe 5；The gravity
Column purification component includes a gravity column fixed plate 6 and multiple gravity columns 7 with protein purification；The gravity column is fixed
Plate 6 is equipped with the multiple and matched U-typed gravity column open slot 8 of the gravity column 7；Each gravity column open slot 8
Inner side is equipped with a pair of of elastic bumps 9；The gravity column 7, which coordinates, is equipped with dismountable chock plug 10；The gravity column 7 is opened equipped with one
Close valve 11；The pressue device 12 is removably connected by the switch valve 11 with the gravity column 7.
As a kind of preferred solution of the present embodiment, the bottom of the base 1 also tiles and has skid resistant course 13；The base 1
On be additionally provided with multiple fixed hole positions 14, when the skid resistant course 13 and/or the fixed hole position 14 avoid motor operational vibration
Position deviation.
The operation principle of the present embodiment is：The output shaft of the first electrode is connected with first screw 4, and described first
The sliding shoe 5 is arranged with screw 4, the sliding shoe 5 moves back and forth on first screw 4, and the gravity column is fixed
The both ends of plate 6 are fixedly linked by the liftway 21 in the rail brackets 2 with sliding shoe 5, the gravity column fixed plate 6 with
The movement of the sliding shoe 5 and move, and then realize the gravity column fixed plate 6 upper-lower position adjust；When the gravity column 7
After long-time use, its flow velocity slows down, and the pressure ball is connected on the gravity column 7, and the chock plug 10 is blocked described
The opening of gravity column 7, opens the switch valve 11, presses the pressure ball and pressurizes into the gravity column 7, improves the gravity
The flow rate of liquid of column 7.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of changes, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (8)
- The gravity column 1. a kind of liftable for protein purification pressurizes, include gravity column holder, gravity column purification component and Gravity column lifting unit, it is characterised in that the gravity column holder includes a base and vertical with the base two guide rails Stent；Two rail bracketses are located at the both ends of the base；The both ends of the gravity column purification component are connected in two respectively On the rail brackets, and " H " type is formed between two rail bracketses；It is equipped with two rail bracketses described Gravity column lifting unit, the both ends of the gravity column purification component pass through the rail brackets and the gravity column lifting unit phase Even；The gravity column purification component includes a gravity column fixed plate and multiple gravity columns with protein purification；It is described Gravity column, which coordinates, is equipped with dismountable chock plug；The gravity column is connected with pressue device.
- The gravity column 2. a kind of liftable for protein purification according to claim 1 pressurizes, it is characterised in that described Rail brackets is in long strip type, and a liftway is additionally provided with the rail brackets, and the liftway is used for described heavy with placement Power column fixed plate.
- The gravity column 3. a kind of liftable for protein purification according to claim 1 pressurizes, it is characterised in that described Gravity column is equipped with a switch valve；The pressue device is removably connected by the switch valve with the gravity column.
- The gravity column 4. a kind of liftable for protein purification according to claim 1 pressurizes, it is characterised in that described Gravity column lifting unit includes the first motor being respectively fixedly connected on two rail bracketses, the first screw and is set in described Sliding shoe on first screw, the both ends of the gravity column fixed plate, which connect, is fixed with the sliding shoe.
- The gravity column 5. a kind of liftable for protein purification according to claim 1 pressurizes, it is characterised in that described Gravity column fixed plate is equipped with the multiple and matched gravity column open slot of the gravity column；The gravity column open slot is in " U " Type.
- The gravity column 6. a kind of liftable for protein purification according to claim 5 pressurizes, it is characterised in that each The inner side of the gravity column open slot is equipped with a pair of of elastic bumps, and the elastic bumps are used to block the gravity column.
- The gravity column 7. a kind of liftable for protein purification according to claim 1 pressurizes, it is characterised in that described Also tile and have skid resistant course in the bottom of base.
- The gravity column 8. a kind of liftable for protein purification according to claim 1 or 7 pressurizes, it is characterised in that Multiple fixed hole positions are additionally provided with the base, multiple fixed hole positions are used to fix the base on the stationary platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711444980.4A CN107903298A (en) | 2017-12-27 | 2017-12-27 | A kind of liftable pressurization gravity column for protein purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711444980.4A CN107903298A (en) | 2017-12-27 | 2017-12-27 | A kind of liftable pressurization gravity column for protein purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107903298A true CN107903298A (en) | 2018-04-13 |
Family
ID=61871608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711444980.4A Withdrawn CN107903298A (en) | 2017-12-27 | 2017-12-27 | A kind of liftable pressurization gravity column for protein purification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107903298A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005192558A (en) * | 2003-10-20 | 2005-07-21 | Fuji Photo Film Co Ltd | Nucleic acid-adsorbing porous membrane for separating and purifying nucleic acid and apparatus for separating and purifying nucleic acid |
| US20090050548A1 (en) * | 2006-08-21 | 2009-02-26 | Hamid Shirkhan | Replaceable column for pressurized liquid extraction system |
| KR101278622B1 (en) * | 2012-09-07 | 2013-06-25 | 대한민국 | Apparatus for purificating column |
| CN203405366U (en) * | 2013-09-06 | 2014-01-22 | 中国农业科学院兰州兽医研究所 | A simple booster for protein purification with prepacked columns |
| CN205953893U (en) * | 2016-08-29 | 2017-02-15 | 中国烟草总公司郑州烟草研究院 | Manual gravity post affinity chromatography condensation sleeve fixing device |
| CN206666433U (en) * | 2017-04-21 | 2017-11-24 | 深圳市国创纳米抗体技术有限公司 | A kind of protein purification device for being suitable for gravity post |
| CN206692665U (en) * | 2017-03-28 | 2017-12-01 | 东莞市佰韧五金电子科技有限公司 | Nucleic acid purification equipment |
-
2017
- 2017-12-27 CN CN201711444980.4A patent/CN107903298A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005192558A (en) * | 2003-10-20 | 2005-07-21 | Fuji Photo Film Co Ltd | Nucleic acid-adsorbing porous membrane for separating and purifying nucleic acid and apparatus for separating and purifying nucleic acid |
| US20090050548A1 (en) * | 2006-08-21 | 2009-02-26 | Hamid Shirkhan | Replaceable column for pressurized liquid extraction system |
| KR101278622B1 (en) * | 2012-09-07 | 2013-06-25 | 대한민국 | Apparatus for purificating column |
| CN203405366U (en) * | 2013-09-06 | 2014-01-22 | 中国农业科学院兰州兽医研究所 | A simple booster for protein purification with prepacked columns |
| CN205953893U (en) * | 2016-08-29 | 2017-02-15 | 中国烟草总公司郑州烟草研究院 | Manual gravity post affinity chromatography condensation sleeve fixing device |
| CN206692665U (en) * | 2017-03-28 | 2017-12-01 | 东莞市佰韧五金电子科技有限公司 | Nucleic acid purification equipment |
| CN206666433U (en) * | 2017-04-21 | 2017-11-24 | 深圳市国创纳米抗体技术有限公司 | A kind of protein purification device for being suitable for gravity post |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180413 |
|
| WW01 | Invention patent application withdrawn after publication |