CN107897850A

CN107897850A - A kind of health care fish sauce and preparation method thereof

Info

Publication number: CN107897850A
Application number: CN201711343409.3A
Authority: CN
Inventors: 罗植升
Original assignee: Liuzhou Fei Sheng Peng Science And Technology Co Ltd
Current assignee: Liuzhou Fei Sheng Peng Science And Technology Co Ltd
Priority date: 2017-12-15
Filing date: 2017-12-15
Publication date: 2018-04-13

Abstract

The invention belongs to food technology field, and in particular to a kind of health care fish sauce and preparation method thereof, including basestocks 70 85% and Chinese medicine additive 15 30%；The basestocks include：Frog ovary, wheat bran, coix seed, defatted soybean, brine, citric acid, water after cashew nut, celery, fish, rice residue, oviposition row's essence；The Chinese medicine additive includes：Alfalfa extract 10 18%, Shorthorned Epimedium P.E 10 20%, Moringa seed extract 8 15%, Olive leaf P.E 8 15%, purslane extract 5 10%, Semen Nelumbinis extract 5 10%, dendrobium candidum extract 5 10%, Cacumen Platycladi extract 3 10%, Radix Glycyrrhizae Preparata extract 5 12%, chicory extract 5 10%.The health care fish sauce of the present invention is not only improved containing abundant amino acid and micronutrient levels and the effect that takes care of health of energy assisting in reducing blood sugar, securely and reliably, without any side effects.

Description

A kind of health care fish sauce and preparation method thereof

【Technical field】

The invention belongs to food technology field, and in particular to a kind of health care fish sauce and preparation method thereof.

【Background technology】

Fish sauce is a kind of limpid brown liquid flavoring, has unique gentle saline taste, is cooking and making food When frequently as flavour enhancer or salt substitute, be the biography that Southeast Asia, Japan and coastal areas of southern China one are liked with the people System flavouring, and gradually liked be subject to Europe and North America consumer.The production of China's tradition fish sauce is distributed in southeastern coast more Area, wherein Shantou and Foochow fish sauce production scale are larger.

With the improvement of people's living standards, people compel the demand of the natural fish sauce made and have health-care efficacy Cut.Existing fish sauce production method is different, has his own strong points.But health fish sauce with strong points is deficienter, and majority is still It is with single nutrient component using fish and shrimp as raw material, it is impossible to meet the health care needs of people.

【The content of the invention】

The object of the present invention is to provide a kind of health care fish sauce and preparation method thereof, health care fish sauce of the invention is not only containing rich Rich amino acid and micronutrient levels improve and the effect that takes care of health of energy assisting in reducing blood sugar, securely and reliably, secondary without any poison Effect.

To achieve these goals, the technical solution adopted by the present invention is as follows：

A kind of health care fish sauce, including basestocks 70-85% and Chinese medicine additive 15-30%；The basestocks are according to weight Percentage includes：Frog ovary after cashew nut 1-3%, celery 1-3%, fish 30-45%, rice residue 3-10%, oviposition row's essence 8-15%, wheat bran 3-8%, coix seed 5-10%, defatted soybean 1.5-5%, brine 5-8%, citric acid 0-0.5%, water 15- 20%；The Chinese medicine additive includes：Alfalfa extract 10-18%, Shorthorned Epimedium P.E 10-20%, moringa seeds extraction Thing 8-15%, Olive leaf P.E 8-15%, purslane extract 5-10%, Semen Nelumbinis extract 5-10%, dendrobium candidum extraction Thing 5-10%, Cacumen Platycladi extract 3-10%, Radix Glycyrrhizae Preparata extract 5-12%, chicory extract 5-10%.

It is further preferred that the basestocks include according to percetage by weight：Cashew nut 2.5%, celery 2.5%, fish 39%th, the frog ovary 10% after rice residue 6%, oviposition row's essence, wheat bran 6%, coix seed 7%, defatted soybean 3.8%, brine 6%th, citric acid 0.2%, water 17%；The Chinese medicine additive includes：Alfalfa extract 14%, Shorthorned Epimedium P.E 15%th, Moringa seed extract 11%, Olive leaf P.E 12%, purslane extract 7%, Semen Nelumbinis extract 6%, dendrobium candidum Extract 8%, Cacumen Platycladi extract 8%, Radix Glycyrrhizae Preparata extract 10%, chicory extract 9%.

A kind of preparation method of health care fish sauce as described above, specifically includes following steps：

(1) preparation of Chinese medicine additive：

A, by alfalfa clean dry to moisture content be 1-5% after carry out ultramicro grinding, cross 100-200 mesh sieves after add 70-80% (v/v) ethanol solution of 2-3 times of weight of raw material extracts 2-3 times, and the time extracted every time is 30-40min, in extraction Assisted Extraction is carried out for the ultrasonic wave of 100-200W power using power, the use of rotating speed is every time 1000-2000r/min after extraction Centrifuge centrifuged, three layers of filtered through gauze are used after centrifugation, merging filtrate, is concentrated under reduced pressure into original volume 1.01 and obtains purple again Russian fenugreek herb activity extract, it is stand-by；

B, 1kg Herba Epimedii blades are put into after being dried in drier and crushed, put into supercritical CO₂In extraction equipment, When extraction 3-4 is small, extracting solution is collected, filter residue is added to 70-80% (v/v) ethanol solution of 3-5 times of filter residue quality, is flowed back Extraction 20-30 minutes, the temperature of reflux is at 65-85 DEG C, then pours into 0.3 μm of ceramic film component with the flow velocity of 0.1-0.2m/s Micro-filtration is carried out, under 0.1-0.2MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, with cross-flow side Formula carries out loop ultrafiltration processing, obtains the Herba Epimedii activity extract that molecular weight ranges are 5000Da-6000Da, stand-by；

C, the water of 2-5 times of volume of moringa seeds addition is smashed to pieces, and the high-tension pulse that electric field strength is 25KV/cm is used after smashing into slurry to pieces Electric field treatment is rushed, the time of pulse is 5 × 10^-6-10×10^-6S, the frequency of pulse is 30-50 hertz, is added after being handled 5-10 times 70-80% (v/v) ethanol solution carries out ultrasonic extraction 10-20 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument For 10A/25W-15A/25W, 0.5 μm of ceramic film component is then poured into the flow velocity of 0.1-0.25m/s and carries out micro-filtration, in 0.1- Under 0.2MPa pressure differentials, successively with the membrane module of molecular cut off 5000000Da, 1O000Da, it is super that circulation is carried out with cross-current flow Filter is handled, and obtains the moringa seeds activity extract that molecular weight ranges are 10000Da-15000Da, stand-by；

D, fresh olive leaf is ground into and the water progress water of 3-5 times of material quality is added after slurry carries 10-20 minutes, connect And thick liquid is obtained by filtration, thick liquid is poured into polyamide chromatography column with the flow velocity of 0.1-0.3m/s often chromatographs, and uses 50-60% (v/v) ethanol solution carries out 3 elutions, and the pressure of elution is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution and is 60-70 DEG C, thickening filtration is then carried out, olive leaf activity extract is obtained after being concentrated under reduced pressure, it is stand-by；

E, purslane herb is cleaned after the picking of morning 8-9 points and cleaned, added quality and carry out water for the water of 3-5 times of herb Carry, thick liquid is obtained after filtering, thick liquid is carried out after being concentrated under reduced pressure into 1.01 times of original volume, adds the 65-75% of 5-10 times of volume (v/v) ethanol solution carries out alcohol extracting 20-30 minutes, takes filtrate to be spray-dried to obtain purslane activity extract after filtering, It is stand-by；

F, lotus seeds are shelled and leaves embryo nelumbinis drying to ultramicro grinding after moisture content 2-5%, added after weighing the powder of 1kg In extractor, add 65-75% (v/v) ethanol solution and carry out refluxing extraction 3 times, add the ethanol of 5 times of powder quality for the first time Solution refluxing extraction 60 minutes, filters to take filtrate, is subsequently added into the ethanol solution refluxing extraction of 3 times of filter residue quality 50 minutes, mistake Leaching filtrate, is subsequently added into the ethanol solution refluxing extraction of 2 times of filter residue quality 30 minutes, filters to take filtrate, by filtrate three times Merge, then carry out being concentrated under reduced pressure into 1.01 times of original volume, be then dried under reduced pressure using pressure Reduction Dryer, crushed 100 mesh Sieve, obtains lotus seeds activity extract, stand-by；

G, the fresh stem of dendrobium candidum is put into mixer and is stirred crushing, then using 65-75% (v/v) ethanol solutions in Temperature is to carry out alcohol extracting at -5--3 DEG C to stay overnight, and uses rotating speed to be centrifuged for the centrifuge of 200-500r/min after overnight Thick liquid is obtained by filtration, when room temperature extraction 1-3 is small after thick liquid is mixed with protease, dendrobium candidum activity extract is obtained by filtration, Stand-by, the addition of protease accounts for the 0.05-0.1% of thick liquid gross mass；

H, blended after cacumen biotae is cleaned using mixer, be subsequently added into 5-10 times of water and carry out refluxing extraction 3 Secondary, when each 1-2 is small, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, add 70-80% (v/v) second Alcoholic solution carried out at 5-8 DEG C alcohol extracting 5-8 it is small when, centrifugation take precipitation spray drying obtain cacumen biotae activity extract, treat With；

I, obtained after honey-fried licorice root stem tuber is crushed add 70-80% (v/v) ethanol solution carry out circulation extraction obtain Extracting solution, is 2.0-3.0 by extracting solution addition salt acid for adjusting pH, is then stirred while heating water bath in extracting solution until go out Existing filiform starts timing and continues stirring 5-10 minutes, filters to take filtrate in time, filter residue repeats said extracted step 2 time, by 3 times Filtrate merge be dried under reduced pressure, crush after can obtain honey-fried licorice root activity extract, it is stand-by；

J, after the flower of witloof is dried, 70-80% (v/v) ethanol solution for adding 5-10 times of volume is carried using Soxhlet Take carry out after device surname extraction 2-3 it is small when, after the chitosan solution for filtering to take the 0.01-0.02% that filtrate adds filtrate gross mass Stirring filters off filtrate and carries out heating concentration, be freeze-dried to obtain witloof activity extract until filtrate clarification, stand-by；

(2) preparation of basestocks：According to frog ovum of the percent by weight by cashew nut, fish, rice residue, oviposition row after smart Nest, wheat bran, coix seed, defatted soybean add water after being crushed, and it is small to carry out boiling 10-20 in the case where pressure is 0.1-0.2Mpa When, will then the celery smashed to pieces be admixed, continued after mixing when progress boiling 10-20 is small under pressure is 0.2-0.3Mpa, Obtain basestocks；

(3) preparation of health care fish sauce：By the stand-by material mixing in step (1), (2) and add brine, soy sauce koji mix plus Enter in jar fermenter, closing fermentation 10-15 days at 25-30 DEG C, then shine cylinder 30-40 days under the sun, then control temperature to protect Holding the fermentation at 30 DEG C, to full 8-10 months, it is 28-30 mother-in-law that then citric acid is added after leaching, filtering, sterilizing and is blent to concentration U.S. degree, precipitation can obtain health care fish sauce.

Further instruction, the concentration of brine described in step (3) are 15-20 mother-in-law U.S. degree.

Further instruction, the humidity of surrounding environment remains 30-40% during the fermentation.

Further instruction, cyanide content is 2.11-2.67mg/Kg in the embryo nelumbinis.

Further instruction, the Thick many candies content of the purslane herb is 10-15mg/g.

Alfalfa Medicagosativa, sweet in flavor, light, cold nature.Energy clearing away the stomach-heat, promoting urination and removing dampness contain 5 kinds of vitamins B, vitamin C, vitamin E, 10 kinds of mineral matters and Protoapigenone, carotenoid, the sour three kinds of distinctive nutrients of plant of phenolic. Protoapigenone：With antioxidation, while cholesterol can be prevented in eparterial deposition, and avoid blood clotting blocking, with Reduce the probability that artery sclerosis occurs.Protoapigenone can also block some hormonal bad activities, and suppression causes inflammation Ferment activity, can also suppress capillary proliferation.Phenolic acid：With antioxidation, a kind of antioxidant can be considered, can prevent Hemostatic Oral Liquid condenses into plate, makes blood without hindrance in unobstructed blood vessel, and reduces the generation of cardiovascular disease.

Herba Epimedii Epimedium brevicornu Maxim., acrid-sweet flavor is warm-natured, walks liver kidney two warp.Have and mend the gate of vitality, strengthening the essence The effect of gas, strengthening the bones and muscles, tonifying kidney and strengthening yang.Containing components such as icariine, volatile oil, ceryl alcohol, phytosterol, tannin, vitamin Es. The excited sexual performance of energy, has animal and promotes gonopoiesis effect.Also be depressured (causing peripheral vasodilation), hypoglycemic, diuresis, Kobadrin and the effect of vitamin E sample.

Olive leaf, sour, sweet and neutral.Clearing heat and detoxicating, relieving sore-throat resolving sputum, promote the production of body fluid to quench thirst.Contain secoiridoid and its glycosides, Huang The components such as ketone and its glycosides, biflavone and its glycosides, low molecule tannin, have good bioactivity, can eliminate tissue to insulin Resistance.

Purslane, it is sour, tremble with fear.Return liver, large intestine channel.It is clearing heat and detoxicating, cooling blood and hemostasis, stop dysentery.For toxic-heat and blood stasis, carbuncle swells malignant boil Sore, eczema, erysipelas, snakebite and bugbite, have blood in stool, hemorrhoid blood, metrostaxis.It is embodied in《Chinese Pharmacopoeia》Page 49.

Lotus seeds, are the dry mature seed of nymphaeaceae plant lotus Nelumbo nucifera Gaertn..It is sweet in flavor, puckery, property It is flat.Returns spleen, kidney, the heart channel of Hang-Shaoyin.It is distributed in China north and south each province.With tonifying spleen and stopping diarrhea, the effect of stop-band, the puckery essence of kidney-nourishing, mental-tranquilization. It is usually used in splenasthenic diarrhea, with, seminal emission, palpitation and insomnia.

Dendrobium candidum, nourishing kidney-yin, and the fire of deficiency type can drop, and it is sweet in flavor, it is slightly cold.Return stomach, kidney channel.For injury thirst, deficiency of food constipation, Abnormal heat is not moved back, and mesh is secretly dim-sighted.

Cacumen biotae, bitter, puckery, cold nature, enters lung, liver, large intestine channel, there is the effect of cooling blood and hemostasis, black beard and hair, cough-relieving asthma. Mainly contain the compounds such as flavones, terpene substances, resin, tannin.

Honey-fried licorice root, exocuticle rufous or taupe brown, it is micro- glossy.For section yellow to buff, cambium ring is obvious, penetrates Line is radial.Slightly stickiness.Have burnt odor gas, it is sweet.Thoughts of returning home warp, lung channel, stomach, the spleen channel.Tonifying spleen and stomach, Yiqi and vein recovery.

Witloof, it is cool in nature, it is mildly bitter flavor, salty.Clearing liver cholagogic, stomach strengthening and digestion promoting, inducing diuresis to remove edema.

In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：

1. it with the addition of the frog ovary after cashew nut, fish, rice residue, oviposition row's essence, wheat bran, the heart of a lotus seed in the basestocks of the application The seed of jog's tears, defatted soybean, enrich the constituent content of basestocks, not only improve the mouthfeel of traditional fish sauce and with more mellow Sauce fragrance so that contain substantial amounts of amino acid in basestocks, be especially to maintain the glutamic acid Glu of delicate flavour, tyrosine Thr, Alanine Ala, lysine Lys, the content of valine Val increase so that fish sauce has denseer fragrance, and this Shen Material combination please complements each other not oxidized or micro- with the effect to extend the shelf life, the nutritional ingredient that can also extend in fish sauce Biological utilisation and reduce nutritional ingredient；So as to improve the quality of fish sauce on the whole；Being rich in celery has Flavonoid substances, terpene Material can add the heath-function of fish sauce, and the Chinese medicine additive by addition, which to be rich in fish sauce, substantial amounts of inanimate matter, The synthesis of hemalbumin, cytochromes and various enzymes is participated in rich in substantial amounts of trace element, ferro element in the Chinese medicine additive of addition, Promote growth, also start shipment oxygen therapy and the effect of nutriment in blood, it is normal that manganese element can play maintenance for human body body Glycometabolism and fat metabolism, and have anti-aging, an oxidation resistant effect, the health-care effect in the application is primarily directed to hypoglycemic Health-care effect, play the role of assisting in reducing blood sugar with addition of the flavonoids in celery, terpene substances.

2. the health care additive added in the application is on the basis of research Traditional Chinese Medicine Coryza Treated by Syndrome Differentiation by the applicant It is upper to refer to modern pharmacological research achievement, by experiment for many years, multiple formulations are screened, have found to type of deficiency of both QI and YIN sugar Urinate the good several drugs of sick therapeutic effect and complete the present invention.In the combination of the additive designed in the present invention, pale reddish brown lucerne Mu clearing away the stomach-heat, promoting urination and removing dampness；Moringa seeds, relieve inflammation or internal heat, bring down a fever, cool blood, removing toxic substances；Herba Epimedii strengthening the essence gas, strengthening the bones and muscles, tonifying kidney and strengthening yang；Olive Olive leaf is clearing heat and detoxicating, relieving sore-throat resolving sputum, promotes the production of body fluid to quench thirst；Purslane is clearing heat and detoxicating, cooling blood and hemostasis, stop dysentery；Lotus seeds have tonifying spleen and stopping diarrhea, The effect of stop-band, the puckery essence of kidney-nourishing, mental-tranquilization；Dendrobium candidum nourishing kidney-yin, and the fire of deficiency type can drop；Cacumen biotae cooling blood and hemostasis, black beard and hair, The effect of cough-relieving is breathed heavily；Honey-fried licorice root tonifying spleen and stomach, Yiqi and vein recovery；Witloof clearing liver cholagogic, stomach strengthening and digestion promoting, inducing diuresis to remove edema；10 taste medicines are total to Same-action, complements each other, and substantial amounts of active ingredient is contained in 10 taste medicines, and the technological means by the application is carried out in extraction Chinese medicine Position active material, rich in the effective active composition that can largely play the role of reduction to blood glucose and micro in active material Element, trace element can play the role of nutrient environment in balanced body to the function of human body, can preferably carry out reduction blood The treatment of sugar；253.12-283.15mg/g containing dietary fiber, saponin 0.51-0.94mg/g, class in alfalfa activity extract Flavones 0.16-1.20mg/g and phenolic acid 0.21-1.86mg/g.0.15- containing icariine in Herba Epimedii activity extract 1.62mg/g, ceryl alcohol 0.11-1.32mg/g, phytosterol 0.15-1.35mg/g, tannin 0.12-1.64mg/g, vitamin E 0.32-1.01mg/g；0.24-1.02mg/g containing secoiridoid, secoiridoid glycoside 0.58- in olive leaf activity extract 1.25mg/g.The 79.29-82.23mg/g of material containing rutin, flavones 1.23-2.36mg/g, alkaloid in lotus seeds activity extract 1.15-2.36mg/g；121.21-135.20mg/g containing polysaccharide, alkaloid 2.35-6.32mg/ in dendrobium candidum activity extract G, a variety of amino acid and trace element.Contain flavones 0.56-1.03mg/g, terpene substances 0.58- in cacumen biotae activity extract 1.05mg/g, tannin 0.75-2.01mg/g.1.45-2.86mg/g containing liquiritin, glycyrrhizic acid in honey-fried licorice root activity extract 0.86-1.12mg/g, glycyrrhetol 0.85-2.12mg/g.0.25-0.75mg/ containing aesculetin in witloof activity extract G, Escosyl 0.25-0.64mg/g, cichoriin 0.41-0.59mg/g, lactucin 0.21-0.65mg/g and mountain lettuce Bitter principle 0.18-0.34mg/g.Containing total sugar content it is 8.2%-11.1% in moringa seeds activity extract, protein contains 36.7%- 38.5%, fat content 34.2%-38.3%, the content of potassium is 552.4-610.5mg/100g, calcium content 98.2- 132.1mg/100g, iron content 2.95-3.14mg/100g.Interacting, complement each other between active material, compatibility is proper, Playing health care fish sauce of the present invention and preparation method thereof can not only be to diabetic's fasting blood sugar, Area under the curve of blood glucose It is more suitable than effect with positive drug group and in part index number and cholesterol, the index of triglycerides reduce and be respectively provided with remarkable result It is upper to be slightly better than positive drug group.And the clinical symptoms of diabetic are released using aggregate measures by the method for diagnosis and treatment, The multiple complications of diabetes are prevented, therapeutic effect is securely and reliably, without any side effects significantly, it can be achieved that treat both principal and secondary aspect of disease.

【Embodiment】

Many details are elaborated in the following description in order to fully understand the present invention.But the present invention can be with Much implement different from other manner described here, those skilled in the art can be in the situation without prejudice to intension of the present invention Under do similar improvement, therefore the present invention is not limited to the specific embodiments disclosed below.

Embodiment 1：

A kind of health care fish sauce, including basestocks 70% and Chinese medicine additive 30%；The basestocks are according to percetage by weight Including：Frog ovary 8%, wheat bran 8% after cashew nut 1%, celery 1%, fish 45%, rice residue 10%, oviposition row's essence, Job's tears Benevolence 5%, defatted soybean 1.5%, brine 5%, citric acid 0.5%, water 15%；The Chinese medicine additive includes：Alfalfa carries Take thing 10%, Shorthorned Epimedium P.E 10%, Moringa seed extract 8%, Olive leaf P.E 15%, purslane extract 5%, lotus Seed extract 10%, dendrobium candidum extract 10%, Cacumen Platycladi extract 10%, Radix Glycyrrhizae Preparata extract 12%, chicory extract 10%.

(1) preparation of Chinese medicine additive：

A, by alfalfa clean dry to moisture content be 1% after carry out ultramicro grinding, cross 100 mesh sieves after add raw material 2 70% (v/v) ethanol solution of times weight extracts 2 times, and the time extracted every time is 30min, the use of power is 100W work(in extraction The ultrasonic wave of rate carries out Assisted Extraction, is centrifuged using the centrifuge that rotating speed is 1000r/min after extraction, made after centrifugation every time With three layers of filtered through gauze, merging filtrate, is concentrated under reduced pressure into original volume 1.01 and obtains alfalfa activity extract again, stand-by；

B, the Herba Epimedii blade that 1kg cyanide contents are 2.56mg/Kg is put into after being dried in drier and crushed, thrown Enter supercritical CO₂In extraction equipment, when extraction 3 is small, extracting solution is collected, filter residue is added to 70% (v/v) second of 3 times of filter residue quality Alcoholic solution, carries out refluxing extraction 20 minutes, and the temperature of reflux is at 65 DEG C, then pours into 0.3 μm of ceramics with the flow velocity of 0.1m/s Membrane module carries out micro-filtration, under 0.1MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, with cross-flow Mode carries out loop ultrafiltration processing, obtains the Herba Epimedii activity extract that molecular weight ranges are 5000Da, stand-by；

C, the water of 2 times of volumes of moringa seeds addition is smashed to pieces, and the high-voltage pulse that electric field strength is 25KV/cm is used after smashing into slurry to pieces Electric field treatment, the time of pulse is 5 × 10^-6S, the frequency of pulse is 30 hertz, and 5 times of 70% (v/v) is added after being handled Ethanol solution carries out ultrasonic extraction 10 minutes using Ultrasound Instrument, and the current strength of Ultrasound Instrument is 10A/25W, then with 0.1m/s's Flow velocity pours into 0.5 μm of ceramic film component and carries out micro-filtration, under 0.1MPa pressure differentials, successively with molecular cut off 5000000Da, The membrane module of 1O000Da, loop ultrafiltration processing is carried out with cross-current flow, is obtained the moringa seeds that molecular weight ranges are 10000Da and is lived Property extract, it is stand-by；

D, fresh olive leaf is ground into and the water of 3 times of material quality is added after slurry carries out water and carry 10 minutes, then mistake Filter obtains thick liquid, and thick liquid is poured into polyamide chromatography column with the flow velocity of 0.1m/s often chromatographs, and uses 50% (v/v) ethanol solution 3 elutions are carried out, it is 60 DEG C that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution, is then carried out dense Contracting filtering, obtains olive leaf activity extract after being concentrated under reduced pressure, stand-by；

E, the purslane herb that Thick many candies content is 10mg/g is cleaned after 8 a.m. picking and cleaned, adding quality is The water that 3 times of herb carries out water and carries, and thick liquid is obtained after filtering, and thick liquid is carried out after being concentrated under reduced pressure into 1.01 times of original volume, adds 5 times 65% (v/v) ethanol solution of volume carries out alcohol extracting 20 minutes, takes filtrate to be spray-dried to obtain purslane activity after filtering Extract, it is stand-by；

F, lotus seeds are shelled and leaves ultramicro grinding after embryo nelumbinis drying to moisture content 2%, weighed to add after the powder of 1kg and carry Take in tank, add 65% (v/v) ethanol solution and carry out refluxing extraction 3 times, the ethanol solution for adding 5 times of powder quality for the first time returns Stream extraction 60 minutes, filters to take filtrate, is subsequently added into the ethanol solution refluxing extraction of 3 times of filter residue quality 50 minutes, filter to take filter Liquid, is subsequently added into the ethanol solution refluxing extraction of 2 times of filter residue quality 30 minutes, filters to take filtrate, and filtrate three times is merged, then Carry out being concentrated under reduced pressure into 1.01 times of original volume, be then dried under reduced pressure using pressure Reduction Dryer, crushed 100 mesh sieves, obtained Lotus seeds activity extract, it is stand-by；

G, the fresh stem of dendrobium candidum is put into mixer and is stirred crushing, then using 65% (v/v) ethanol solution in temperature Spend and stayed overnight to carry out alcohol extracting at -5 DEG C, use rotating speed to be centrifuged for the centrifuge of 200r/min after overnight and be obtained by filtration slightly Liquid, when room temperature extraction 1 is small after thick liquid is mixed with protease, is obtained by filtration dendrobium candidum activity extract, stand-by, protease Addition accounts for the 0.05% of thick liquid gross mass；

H, blended after cacumen biotae is cleaned using mixer, be subsequently added into 5 times of water and carry out refluxing extraction 3 times, Every time 1 it is small when, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, add 70% (v/v) ethanol solution in When progress alcohol extracting 5 is small at 5 DEG C, centrifugation takes precipitation spray drying to obtain cacumen biotae activity extract, stand-by；

I, obtained after honey-fried licorice root stem tuber is crushed addition 70% (v/v) ethanol solution carry out circulation extraction extracted Liquid, it is 2.0 that extracting solution is added salt acid for adjusting pH, is then stirred while heating water bath in extracting solution until filiform occur Start timing and continue stirring 5 minutes, filter to take filtrate in time, filter residue repeats said extracted step 2 time, and the filtrate of 3 times is merged Be dried under reduced pressure, crush after can obtain honey-fried licorice root activity extract, it is stand-by；

J, after the flower of witloof is dried, 70% (v/v) ethanol solution for adding 5 times of volumes is used after apparatus,Soxhlet's When progress surname extraction 2 is small, filters to take after filtrate adds 0.01% chitosan solution of filtrate gross mass and stir until filtrate Clarification, filters off filtrate and carries out heating concentration, be freeze-dried to obtain witloof activity extract, stand-by；

(2) preparation of basestocks：According to frog ovum of the percent by weight by cashew nut, fish, rice residue, oviposition row after smart Nest, wheat bran, coix seed, defatted soybean add water after being crushed, and when progress boiling 10 is small under pressure is 0.1Mpa, then will The celery smashed to pieces is admixed, continues, when progress boiling 10 is small under pressure is 0.2Mpa, to obtain basestocks after mixing；

(3) preparation of health care fish sauce：By the stand-by material mixing in step (1), (2) and add the salt that concentration is 15 mother-in-law U.S. degree Water, soy sauce koji mixing are added in jar fermenter, closing fermentation 10 days in the case where the humidity of surrounding environment remains 30%, 25 DEG C, then Cylinder is shone under the sun 30 days, then control the humidity of surrounding environment to remain 30%, temperature and be maintained at 30 DEG C and ferment to 8 full Month, then after leaching, filtering, sterilizing add citric acid blend to concentration for 28 mother-in-law U.S. degree, precipitation can obtain health care fish sauce.

Embodiment 2：

A kind of health care fish sauce, including basestocks 85% and Chinese medicine additive 15%；The basestocks are according to percetage by weight Including：Frog ovary 15%, wheat bran 3% after cashew nut 3%, celery 3%, fish 30%, rice residue 3%, oviposition row's essence, Job's tears Benevolence 10%, defatted soybean 5%, brine 8%, citric acid 0%, water 20%；The Chinese medicine additive includes：Alfalfa extract 18%th, Shorthorned Epimedium P.E 20%, Moringa seed extract 15%, Olive leaf P.E 8%, purslane extract 10%, lotus seeds Extract 5%, dendrobium candidum extract 5%, Cacumen Platycladi extract 4%, Radix Glycyrrhizae Preparata extract 5%, chicory extract 10%.

(1) preparation of Chinese medicine additive：

A, by alfalfa clean dry to moisture content be 5% after carry out ultramicro grinding, cross 200 mesh sieves after add raw material 3 80% (v/v) ethanol solution of times weight extracts 3 times, and the time extracted every time is 40min, the use of power is 200W work(in extraction The ultrasonic wave of rate carries out Assisted Extraction, is centrifuged using the centrifuge that rotating speed is 2000r/min after extraction, made after centrifugation every time With three layers of filtered through gauze, merging filtrate, is concentrated under reduced pressure into original volume 1.01 and obtains alfalfa activity extract again, stand-by；

B, the Herba Epimedii blade that 1kg cyanide contents are 3.12mg/Kg is put into after being dried in drier and crushed, thrown Enter supercritical CO₂In extraction equipment, when extraction 4 is small, extracting solution is collected, filter residue is added to 80% (v/v) second of 5 times of filter residue quality Alcoholic solution, carries out refluxing extraction 30 minutes, and the temperature of reflux is at 85 DEG C, then pours into 0.3 μm of ceramics with the flow velocity of 0.2m/s Membrane module carries out micro-filtration, under 0.2MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, with cross-flow Mode carries out loop ultrafiltration processing, obtains the Herba Epimedii activity extract that molecular weight ranges are 6000Da, stand-by；

C, the water of 5 times of volumes of moringa seeds addition is smashed to pieces, and the high-voltage pulse that electric field strength is 25KV/cm is used after smashing into slurry to pieces Electric field treatment, the time of pulse is 10 × 10^-6S, the frequency of pulse is 50 hertz, and 10 times of 80% (v/ is added after being handled V) ethanol solution carries out ultrasonic extraction 20 minutes using Ultrasound Instrument, and the current strength of Ultrasound Instrument is 15A/25W, then with 0.25m/ The flow velocity of s pours into 0.5 μm of ceramic film component and carries out micro-filtration, under 0.2MPa pressure differentials, uses molecular cut off successively The membrane module of 5000000Da, 1O000Da, carry out loop ultrafiltration processing, it is 15000Da to obtain molecular weight ranges with cross-current flow Moringa seeds activity extract, it is stand-by；

D, fresh olive leaf is ground into and the water of 5 times of material quality is added after slurry carries out water and carry 20 minutes, then mistake Filter obtains thick liquid, and thick liquid is poured into polyamide chromatography column with the flow velocity of 0.3m/s often chromatographs, and uses 60% (v/v) ethanol solution 3 elutions are carried out, it is 70 DEG C that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution, is then carried out dense Contracting filtering, obtains olive leaf activity extract after being concentrated under reduced pressure, stand-by；

E, the purslane herb that Thick many candies content is 15mg/g is cleaned after 9 points of pickings of the morning and cleaned, adding quality is The water that 5 times of herb carries out water and carries, and thick liquid is obtained after filtering, and thick liquid is carried out after being concentrated under reduced pressure into 1.01 times of original volume, adds 10 75% (v/v) ethanol solution of times volume carries out alcohol extracting 30 minutes, takes filtrate to be spray-dried to obtain purslane work after filtering Property extract, it is stand-by；

F, lotus seeds are shelled and leaves ultramicro grinding after embryo nelumbinis drying to moisture content 5%, weighed to add after the powder of 1kg and carry Take in tank, add 75% (v/v) ethanol solution and carry out refluxing extraction 3 times, the ethanol solution for adding 5 times of powder quality for the first time returns Stream extraction 60 minutes, filters to take filtrate, is subsequently added into the ethanol solution refluxing extraction of 3 times of filter residue quality 50 minutes, filter to take filter Liquid, is subsequently added into the ethanol solution refluxing extraction of 2 times of filter residue quality 30 minutes, filters to take filtrate, and filtrate three times is merged, then Carry out being concentrated under reduced pressure into 1.01 times of original volume, be then dried under reduced pressure using pressure Reduction Dryer, crushed 100 mesh sieves, obtained Lotus seeds activity extract, it is stand-by；

G, the fresh stem of dendrobium candidum is put into mixer and is stirred crushing, then using 75% (v/v) ethanol solution in temperature Spend and stayed overnight to carry out alcohol extracting at -3 DEG C, use rotating speed to be centrifuged for the centrifuge of 500r/min after overnight and be obtained by filtration slightly Liquid, when room temperature extraction 3 is small after thick liquid is mixed with protease, is obtained by filtration dendrobium candidum activity extract, stand-by, protease Addition accounts for the 0.1% of thick liquid gross mass；

H, blended after cacumen biotae is cleaned using mixer, be subsequently added into 10 times of water and carry out refluxing extraction 3 times, Every time 2 it is small when, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, add 80% (v/v) ethanol solution in When progress alcohol extracting 8 is small at 8 DEG C, centrifugation takes precipitation spray drying to obtain cacumen biotae activity extract, stand-by；

I, obtained after honey-fried licorice root stem tuber is crushed addition 80% (v/v) ethanol solution carry out circulation extraction extracted Liquid, it is 3.0 that extracting solution is added salt acid for adjusting pH, is then stirred while heating water bath in extracting solution until filiform occur Start timing and continue stirring 10 minutes, filter to take filtrate in time, filter residue repeats said extracted step 2 time, and the filtrate of 3 times is merged Be dried under reduced pressure, crush after can obtain honey-fried licorice root activity extract, it is stand-by；

J, after the flower of witloof is dried, 80% (v/v) ethanol solution for adding 10 times of volumes uses apparatus,Soxhlet's When progress surname extraction 3 is small afterwards, filters to take after filtrate adds 0.02% chitosan solution of filtrate gross mass and stir until filter Liquid is clarified, and is filtered off filtrate and is carried out heating concentration, is freeze-dried to obtain witloof activity extract, stand-by；

(2) preparation of basestocks：According to frog ovum of the percent by weight by cashew nut, fish, rice residue, oviposition row after smart Nest, wheat bran, coix seed, defatted soybean add water after being crushed, and when progress boiling 20 is small under pressure is 0.2Mpa, then will The celery smashed to pieces is admixed, continues, when progress boiling 20 is small under pressure is 0.3Mpa, to obtain basestocks after mixing；

(3) preparation of health care fish sauce：By the stand-by material mixing in step (1), (2) and add the salt that concentration is 20 mother-in-law U.S. degree Water, soy sauce koji mixing are added in jar fermenter, closing fermentation 15 days in the case where the humidity of surrounding environment remains 40%, 30 DEG C, then Cylinder is shone under the sun 40 days, then control the humidity of surrounding environment to remain 40%, temperature and be maintained at 30 DEG C and ferment to full 10 A month, then after leaching, filtering, sterilizing add citric acid blend to concentration for 30 mother-in-law U.S. degree, precipitation can obtain health care fish sauce.

Embodiment 3：

A kind of health care fish sauce, including basestocks 80% and Chinese medicine additive 20%；The basestocks are according to percetage by weight Including：Cashew nut 2.5%, celery 2.5%, fish 39%, rice residue 6%, oviposition row essence after frog ovary 10%, wheat bran 6%, Coix seed 7%, defatted soybean 3.8%, brine 6%, citric acid 0.2%, water 17%；The Chinese medicine additive includes：Pale reddish brown lucerne Mu extract 14%, Shorthorned Epimedium P.E 15%, Moringa seed extract 11%, Olive leaf P.E 12%, purslane extract 7%th, Semen Nelumbinis extract 6%, dendrobium candidum extract 8%, Cacumen Platycladi extract 8%, Radix Glycyrrhizae Preparata extract 10%, witloof extraction Thing 9%.

(1) preparation of Chinese medicine additive：

A, by alfalfa clean dry to moisture content be 3% after carry out ultramicro grinding, cross 150 mesh sieves after add raw material 2 75% (v/v) ethanol solution of times weight extracts 3 times, and the time extracted every time is 35min, the use of power is 150W work(in extraction The ultrasonic wave of rate carries out Assisted Extraction, is centrifuged using the centrifuge that rotating speed is 1500r/min after extraction, made after centrifugation every time With three layers of filtered through gauze, merging filtrate, is concentrated under reduced pressure into original volume 1.01 and obtains alfalfa activity extract again, stand-by；

B, the Herba Epimedii blade that 1kg cyanide contents are 2.96mg/Kg is put into after being dried in drier and crushed, thrown Enter supercritical CO₂In extraction equipment, when extraction 3.5 is small, extracting solution is collected, filter residue is added to 75% (v/v) of 4 times of filter residue quality Ethanol solution, carries out refluxing extraction 25 minutes, and the temperature of reflux is at 70 DEG C, then pours into 0.3 μm of pottery with the flow velocity of 0.15m/s Porcelain membrane module carries out micro-filtration, under 0.15MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, with Cross-current flow carries out loop ultrafiltration processing, obtains the Herba Epimedii activity extract that molecular weight ranges are 5500Da, stand-by；

C, the water of 3 times of volumes of moringa seeds addition is smashed to pieces, and the high-voltage pulse that electric field strength is 25KV/cm is used after smashing into slurry to pieces Electric field treatment, the time of pulse is 7 × 10^-6S, the frequency of pulse is 40 hertz, and 8 times of 77% (v/v) is added after being handled Ethanol solution carries out ultrasonic extraction 15 minutes using Ultrasound Instrument, and the current strength of Ultrasound Instrument is 12A/25W, then with 0.2m/s's Flow velocity pours into 0.5 μm of ceramic film component and carries out micro-filtration, under 0.15MPa pressure differentials, successively with molecular cut off 5000000Da, The membrane module of 1O000Da, loop ultrafiltration processing is carried out with cross-current flow, is obtained the moringa seeds that molecular weight ranges are 12000Da and is lived Property extract, it is stand-by；

D, fresh olive leaf is ground into and the water of 4 times of material quality is added after slurry carries out water and carry 15 minutes, then mistake Filter obtains thick liquid, and thick liquid is poured into polyamide chromatography column with the flow velocity of 0.2m/s often chromatographs, and uses 55% (v/v) ethanol solution 3 elutions are carried out, it is 65 DEG C that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution, is then carried out dense Contracting filtering, obtains olive leaf activity extract after being concentrated under reduced pressure, stand-by；

E, the purslane herb that Thick many candies content is 12mg/g is cleaned after 8 a.m. picking and cleaned, adding quality is The water that 4 times of herb carries out water and carries, and thick liquid is obtained after filtering, and thick liquid is carried out after being concentrated under reduced pressure into 1.01 times of original volume, adds 8 times 70% (v/v) ethanol solution of volume carries out alcohol extracting 25 minutes, takes filtrate to be spray-dried to obtain purslane activity after filtering Extract, it is stand-by；

F, lotus seeds are shelled and leaves ultramicro grinding after embryo nelumbinis drying to moisture content 3%, weighed to add after the powder of 1kg and carry Take in tank, add 70% (v/v) ethanol solution and carry out refluxing extraction 3 times, the ethanol solution for adding 5 times of powder quality for the first time returns Stream extraction 60 minutes, filters to take filtrate, is subsequently added into the ethanol solution refluxing extraction of 3 times of filter residue quality 50 minutes, filter to take filter Liquid, is subsequently added into the ethanol solution refluxing extraction of 2 times of filter residue quality 30 minutes, filters to take filtrate, and filtrate three times is merged, then Carry out being concentrated under reduced pressure into 1.01 times of original volume, be then dried under reduced pressure using pressure Reduction Dryer, crushed 100 mesh sieves, obtained Lotus seeds activity extract, it is stand-by；

G, the fresh stem of dendrobium candidum is put into mixer and is stirred crushing, then using 70% (v/v) ethanol solution in temperature Spend and stayed overnight to carry out alcohol extracting at -4 DEG C, use rotating speed to be centrifuged for the centrifuge of 300r/min after overnight and be obtained by filtration slightly Liquid, when room temperature extraction 2 is small after thick liquid is mixed with protease, is obtained by filtration dendrobium candidum activity extract, stand-by, protease Addition accounts for the 0.08% of thick liquid gross mass；

H, blended after cacumen biotae is cleaned using mixer, be subsequently added into 7 times of water and carry out refluxing extraction 3 times, Every time 1 it is small when, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, add 76% (v/v) ethanol solution in When progress alcohol extracting 6 is small at 6 DEG C, centrifugation takes precipitation spray drying to obtain cacumen biotae activity extract, stand-by；

I, obtained after honey-fried licorice root stem tuber is crushed addition 77% (v/v) ethanol solution carry out circulation extraction extracted Liquid, it is 2.5 that extracting solution is added salt acid for adjusting pH, is then stirred while heating water bath in extracting solution until filiform occur Start timing and continue stirring 8 minutes, filter to take filtrate in time, filter residue repeats said extracted step 2 time, and the filtrate of 3 times is merged Be dried under reduced pressure, crush after can obtain honey-fried licorice root activity extract, it is stand-by；

J, after the flower of witloof is dried, 75% (v/v) ethanol solution for adding 8 times of volumes is used after apparatus,Soxhlet's When progress surname extraction 2.5 is small, filters to take after filtrate adds 0.015% chitosan solution of filtrate gross mass and stir until filter Liquid is clarified, and is filtered off filtrate and is carried out heating concentration, is freeze-dried to obtain witloof activity extract, stand-by；

(2) preparation of basestocks：According to frog ovum of the percent by weight by cashew nut, fish, rice residue, oviposition row after smart Nest, wheat bran, coix seed, defatted soybean add water after being crushed, when progress boiling 15 is small under pressure is 0.15Mpa, then The celery smashed to pieces will be admixed, continue, when progress boiling 15 is small under pressure is 0.25Mpa, to obtain basestocks after mixing；

(3) preparation of health care fish sauce：By the stand-by material mixing in step (1), (2) and add the salt that concentration is 18 mother-in-law U.S. degree Water, soy sauce koji mixing are added in jar fermenter, closing fermentation 13 days in the case where the humidity of surrounding environment remains 36%, 28 DEG C, then Cylinder is shone under the sun 35 days, then control the humidity of surrounding environment to remain 35%, temperature and be maintained at 30 DEG C and ferment to 9 full Month, then after leaching, filtering, sterilizing add citric acid blend to concentration for 29 mother-in-law U.S. degree, precipitation can obtain health care fish sauce.

The health-care effect verification experimental verification of Chinese medicine additive：

1.1 principle

1-3 of the embodiment of the present invention, comparative example, 0.9% physiological saline (medicine company Group Co., Ltd of Nanning Baihui), four Oxygen pyrimidine (Sigma Co., USA), metformin hydrochloride tablet (Shanghai Shi Guibao pharmaceutical Co. Ltds of Sino-U.S.), blood glucose meter and blood glucose Test paper (Japanese Omron Corp), medical starch (Zhengzhou Hua Yao bio tech ltd).

1.2 build group

Healthy male rat (150 ± 20g) Guangxi University of Chinese Medicine Experimental Animal Center is bought to provide, it is common to maintain material suitable It should raise 5 days, when fasting 4 is small, take tail blood, measure i.e. to when small (0) blood glucose value before glucose, gives 2.5g/kgBW grapes on an empty stomach Blood glucose value when measure 0.5,2 is small after sugar, as the batch animal basic value.With 0,0.5 it is small when blood sugar level point 14 groups, convert into 2g/kg crude drug amounts are administered, and distilled water dissolving, once, each experimental group is seen below daily gastric infusion, every group 10.Blank control Group does not process, and 11 experimental group gavages give different given the test agent, and model control group gives same volume solvent, continuous 35 days. Each group replaces high lipid food after giving the raising of maintenance material 1 week in addition to blank control group, after continuing feeding 3 weeks, except blank control group Outer fasting 24 (can't help water) when small, give STZ 30mg/kg BW intraperitoneal injections, injection volume 1ml/100g weight.Continue after injection High lipid food is given to be fed with 5 days.Off-test, when each group animal fasting 4 is small, detection fasting blood-glucose, sugar tolerance, serum insulin And cholesterol, triglyceride level.

The situation of experimental group is as follows：

1 model control group (high lipid food is fed, and gives same volume solvent gastric infusion, once a day)

1 blank control group (not processing)；

(diabetes pill, dosage 0.5g/kg, the dose,equivalent of rat is equivalent to human body recommended amounts for 1 positive drug control group 5 times, daily equivalent to adult human dose 6g/, once a day)；

1 A alfalfa extracts control group (equivalent 2g/kg crude drug amounts administration, one time a day)；1 B Herba Epimedii extraction Thing control group (equivalent 2g/kg crude drug amounts administration, one time a day)；1 C Moringa seed extracts control group (equivalent 2g/kg crude drug amount Administration, one time a day)；1 D Olive leaf P.Es control group (equivalent 2g/kg crude drug amounts administration, one time a day)；1 E purslane Extract control group (equivalent 2g/kg crude drug amounts administration, one time a day)；1 F Semen Nelumbinis extracts control group (equivalent 2g/kg crude drug Amount administration, one time a day)；1 G dendrobium candidum extracts control group (equivalent 2g/kg crude drug amounts administration, one time a day)；1 H side CedarLeaves extract control group (equivalent 2g/kg crude drug amounts administration, one time a day)；1 I Radix Glycyrrhizae Preparata extracts control group (equivalent 2g/ Kg crude drug amounts are administered, one time a day)；1 J chicory extracts control group (equivalent 2g/kg crude drug amounts administration, one time a day)；1 reality Apply 1 Chinese medicine additive composition of example (equivalent 2g/kg crude drug amounts administration, one time a day).

2.1 observation index

2.1.1 fasting blood-glucose, sugar tolerance

When each group animal fasting 4 is small, measure fasting blood-glucose is given not to when small (0) blood glucose value, dosage group before glucose With concentration of test sample, model control group gives same volume solvent, and blank control group does not process, and each group is orally given after twenty minutes Glucose 2.5g/kg BW are given, blood glucose value when small to each group after glucose 0.5,2 are measured, if blood when model control group 0.5 is small Sugared value >=10mmol/L, or when model control group 0.5 is small, 2 it is small when any time point blood glucose rise or Area under the curve of blood glucose liter Height, compared with blank control group, difference has conspicuousness, and decision model carbohydrate metabolism disturbance is set up, on this basis, observing and nursing pair According to group with test sample group fasting blood-glucose, to when small (0.5,2) blood glucose after glucose and 0,0.5,2 it is small when Area under the curve of blood glucose Change.

Blood glucose value × 100% before blood glucose rate of descent %=(blood glucose value after blood glucose value-experiment before experiment)/experiment

Area under the curve of blood glucose==1/2 × (0 it is small when blood glucose+0.5 it is small when blood glucose) × 0.5+1/2 × (0.5 it is small when blood glucose + 1 it is small when blood glucose) × 0.5+1/2 × (1 it is small when blood glucose+2 it is small when blood glucose) × 1+1/2 × (2 it is small when blood glucose+3 it is small when blood glucose) × 1

2.1.2 cholesterol, triglyceride

When each group animal fasting 4 is small, detection serum cholesterol, triglyceride, if model control group serum cholesterol or sweet Oily three esters are significantly raised, and compared with blank control group, difference has conspicuousness, and decision model disorders of lipid metabolism is set up, basic herein On, observing and nursing control group and test sample group Blood Lipid.

2.1.3 insulin

When each group animal fasting 4 is small, serum insulin, model control group insulin resistance compared with blank control group are detected Index is without being decreased obviously, and animal sugar/disorders of lipid metabolism is set up, judge insulin resistance sugar hyperlipoidemia success.See Examine model control group and test sample group insulin resistance condition.

Insulin resistance index=insulin/22.5e^{- ㏑ blood glucose}≈ (blood glucose × insulin)/22.5

2.1.4 data processing and result judgement

Using variance analysis, but homogeneity test of variance need to be first carried out by the program of variance analysis, variance is neat, calculates F values, F Value<F0.05, conclusion：No significant difference between each group mean；F values >=F0.05, P≤0.05 are right with multiple experimental groups and one Counted according to the comparative approach two-by-two of mean between group.

3.1 data processings and result judgement

Raising 5 days, fasting blood sugar when fasting 4 is small is shown in Table 1：

Table 1

Significance analysis：*：With model group than * P<0.05, * * P<0.01；#：Compare #P with positive drug group<0.05, ##P< 0.01。

The testing result table of resistance to sugar amount is shown in Table 2：

Table 2

Significance analysis：*:With model than * P<0.05, * * P<0.01；#：Compare #P with positive drug group<0.05, ##P<0.01.

T-CHOL, triglyceride determination result hand over table 3

Table 3

It can be seen that by table 1-3 by measurement results such as fasting blood sugar, sugar tolerance, cholesterol, triglycerides.Fasting blood-glucose In value, the high low dose group of composition has significant difference with model group ratio.Had using the Chinese medicine additive of the application fine Hypoglycemic effect, the embodiment that compares 1 and A-J groups, single addition alfalfa extract, Shorthorned Epimedium P.E, Moringa Seed extract, Olive leaf P.E, purslane extract, Semen Nelumbinis extract, dendrobium candidum extract, Cacumen Platycladi extract, process Licorice or chicory extract are arrived the significant effect of embodiment 1 in hypoglycemic.

Chinese medicine additive is added during garos in the present invention and carries out raw material, after 10 taste medicines carry out extraction active portion Share, can effectively reach reduction blood glucose, prevention and the effect for improving diabetic complication.

4.1 toxicological test：

Acute toxicity test：Mouse, rat limosis gavage product of the present invention, are observed 7 days, male and female mouse and rat are showed no Poisoning symptom, no death, product male and female mouse of the present invention and rat peroral acute toxicity experiment MTD>It is 10.0g/kg.BW true The nontoxic level in border.

With the medicament of E1 groups in the application to mouse, rat limosis gavage, thing is dead after observation 14 days, to male and female mouse and Rat is showed no poisoning symptom, no death, and animal subject is not put to death and carries out dissection inspection by the observation period, liver, board, kidney, stomach, intestines, The main organs such as the heart, lung, show no obvious abnormalities change.Product male and female mouse of the present invention and rat peroral acute toxicity experiment MTD> 10.0g/kg.BW according to《Health food is examined and assessment technique specification)) acute toxicity grading criteria in (version in 2003), Belong to nontoxic level.

Product Salmonella reversion test of the present invention, mouse marrow cell micro nuclear test and mouse sperm distortion test result are the moon Property, prompt product safety of the present invention.

During 30 days feeding trials of product of the present invention, the activity of animal, ingest, the ordinary circumstance such as defecation has no obvious different Often, and the weight to animal, food-intake, routine blood indexes, organ weights have no significant effect, and are in female male rat high dose group Items Testing index does not observe anomalous variation during 20.00g/kg.BW (equivalent to 500 times of human body recommended amounts).

5.1 clinical test

Quench one's thirst to the present invention treatment deficiency of both qi and yin disease patient the effect of carry out clinical observation, pass through and contrast that medication is front and rear to suffer from Person uses the amount analysis of insulin hypoglycemic effect of the invention.

5.1.1 inclusive criteria

All have that polydipsia, the paddy that disappears are easily hungry, urine is more and sweet tea, body are gradually shown in that patient of becoming thin can be diagnosed as diabete (diabetes)；All lassitude hypodynamias, the lazy speech of spontaneous sweating, shortness of breath, thirst and liking drink, dysphoria in chestpalms-soles, palpitation and insomnia, urinate red constipation, tongue HongShaoJin(SIC), tongue body fat big, tongue are thin or flower is shelled, small and wiry pulse or number person, are Qi and Yin deficiency diabetes mellitus；There is diabetic symptom, appoint When between blood glucose ＞ 11.1mmol/L or fasting blood-glucose ＞ 7.0mmol/L；Diabetes B is had been determined as, long-term use of diformazan is double The patient of guanidine or the patient of long term injections insulin；All patients meet 1 or more than 1 in above-mentioned 4.

5.1.2 case selection

It is all while meet above-mentioned doctor trained in Western medicine criteria for classification, TCM syndrome diagnostic criteria patient；Age 18-65 year person；It is ready to connect By 2 weeks curers；Signature enters clinical research informed consent form person.

5.1.3 medicine

By the Chinese medicine additive 10g in embodiment 1, it is dissolved in 100ml water and obtains test solvent a；Regular iletin：River The biochemical medical limited companies of Su Wanbang, lot number：1007217.

5.1.4 clinical test is grouped

Comparative example group：Insulin；Test example group：Test solvent a+ insulin.

5.1.5 observation index：Observe the situation of change of main clinic symptoms and sign, on an empty stomach and it is postprandial 2 it is small when blood glucose examine Survey；The decrement of insulin dosage.

5.1.6 treatment method：

5.1.6.1 start before administration must continuous 3 inspections blood glucose mean be fasting blood-glucose ＞ 7.0mmol/L, postprandial blood Sugar is ＞ 11.1mmol/L；Other any drops must be disabled before meeting 100 clothes for patients development clinical test of inclusion criteria The Chinese and Western medicine of sugar.

5.1.6.2 100 patients of comparative example group continue to use insulin；100 clothes for patients test solvents of test example group A, each 20ml, 3 times a day, 30 minutes before meals oral, and 2 weeks are 1 course for the treatment of, and therapeutic process is with the use of insulin control blood glucose In critical field.

5.1.6.3 therapeutic process strictly carries out dietetic treatment.

5.1.7 result：Test group patient is gradually reduced in 2 weeks or so blood glucose of taking medicine, and insulin usage amount gradually reduces, drop Sugar effect is steady, and the observation to safety indexes shows, medicine of the present invention has no adverse reaction.Insulin dosage is shown in after clinical treatment Following table.

Insulin dosage (x ± s) (n=100) after table 4 is treated

Group	Insulin dosage
		Contrast groups	55±1.2U
Experimental group	25±0.8U

Test example group is compared with comparative example group, P<0.01.

The result shows that：With the use of the dosage of insulin while treatment using the present invention, controlled with insulin is used alone Dosage during treatment is compared, and has pole significant difference (P<0.01), illustrate that Chinese medicine composition of the present invention suffers from type of deficiency of both QI and YIN diabetes Person has notable hypoglycemic effect, its insulin dosage is substantially reduced to insulin-dependent patient.

And two courses for the treatment of are carried out to 100 patients for taking test solvent a and take rear physical examination, result of the test shows, takes After two courses for the treatment of, type of deficiency of both QI and YIN diabetes patient clinical symptom can be obviously improved, reduces fasting blood-glucose and 2h-plasma glucose (P< 0.05)；Blood, urine, stool and hepatic and renal function items Testing index are in normal range (NR), the medicine of prompting test solvent a before and after test-meal Thing, without obvious infringement, has treatment diabetes effect to tested patients' health.

2nd, the Quality Detection of fish sauce：

Sample to be detected is：

Embodiment 1, embodiment 2, embodiment 3, control group：The a certain packed packaging fish sauce purchased on the market.For 4 groups of fishes Reveal and carry out composition detection on December 11st, 1, carry out composition detection after preventing 3 months again.

The project of detection is as follows：

The amino acid content of free state in fish sauce, measures with reference to GB/5009.124-203.Ferro element, manganese element content Use aas determination.

5 comparison of ingredients table g/100mL of table

As seen from the above table, in glutamic acid Glu, junket in the fish sauce quality that takes care of health being prepared using the application technique and raw material Propylhomoserin Thr, alanine Ala, lysine Lys, valine Val, ferro element, the content of manganese element are higher than the content of comparative example, Illustrate that quality is more excellent, and after 3 months place, glutamic acid Glu, tyrosine Thr, alanine Ala in comparative example, rely Propylhomoserin Lys, valine Val, ferro element, the content decrement of manganese element are higher than embodiment 1-3, it was demonstrated that the technique of the application with The fish sauce that raw material is prepared has the economic benefit that preferably stores, and the shelf-life comparative example that compares is more permanent；The application's Raw material has broken the limitation of the prior art, with the addition of plurality of raw materials and the content for not only improving trace element is prepared, Chinese medicine adds Add agent that there is certain inoxidizability after combining, and the harmful microorganism in fish sauce can be resisted to reduce fish sauce by harmful microorganism profit With and reduce the nutrient composition content of fish sauce.

Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

A kind of 1. health care fish sauce, it is characterised in that：Including basestocks 70-85% and Chinese medicine additive 15-30%；The basestocks Include according to percetage by weight：After cashew nut 1-3%, celery 1-3%, fish 30-45%, rice residue 3-10%, oviposition row's essence Frog ovary 8-15%, wheat bran 3-8%, coix seed 5-10%, defatted soybean 1.5-5%, brine 5-8%, citric acid 0- 0.5%th, water 15-20%；The Chinese medicine additive includes：Alfalfa extract 10-18%, Shorthorned Epimedium P.E 10-20%, Moringa seed extract 8-15%, Olive leaf P.E 8-15%, purslane extract 5-10%, Semen Nelumbinis extract 5-10%, iron Skin Herba Dendrobii extract 5-10%, Cacumen Platycladi extract 3-10%, Radix Glycyrrhizae Preparata extract 5-12%, chicory extract 5-10%.
A kind of 2. health care fish sauce as claimed in claim 1, it is characterised in that：The basestocks include according to percetage by weight： Frog ovary 10%, wheat bran 6% after cashew nut 2.5%, celery 2.5%, fish 39%, rice residue 6%, oviposition row's essence, Job's tears Benevolence 7%, defatted soybean 3.8%, brine 6%, citric acid 0.2%, water 17%；The Chinese medicine additive includes：Alfalfa carries Take thing 14%, Shorthorned Epimedium P.E 15%, Moringa seed extract 11%, Olive leaf P.E 12%, purslane extract 7%, Semen Nelumbinis extract 6%, dendrobium candidum extract 8%, Cacumen Platycladi extract 8%, Radix Glycyrrhizae Preparata extract 10%, chicory extract 9%.
A kind of 3. preparation method of health care fish sauce as described in claim 1-2 any one, it is characterised in that：Specifically include following Step：

(1) preparation of Chinese medicine additive：

A, by alfalfa clean dry to moisture content be 1-5% after carry out ultramicro grinding, cross 100-200 mesh sieves after add raw material 70-80% (v/v) ethanol solution of 2-3 times of weight extracts 2-3 times, and the time extracted every time is 30-40min, is used in extraction Power for 100-200W power ultrasonic wave carry out Assisted Extraction, every time extraction after using rotating speed be 1000-2000r/min from Scheming is centrifuged, and three layers of filtered through gauze is used after centrifugation, merging filtrate, is concentrated under reduced pressure into original volume 1.01 and obtains pale reddish brown lucerne again Mu activity extract, it is stand-by；

B, 1kg Herba Epimedii blades are put into after being dried in drier and crushed, put into supercritical CO₂In extraction equipment, 3- is extracted 4 it is small when, collect extracting solution, filter residue added to 70-80% (v/v) ethanol solution of 3-5 times of filter residue quality, progress refluxing extraction 20-30 minutes, the temperature of reflux was at 65-85 DEG C, then pours into 0.3 μm of ceramic film component with the flow velocity of 0.1-0.2m/s and carries out Micro-filtration, under 0.1-0.2MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, with cross-current flow into The processing of row loop ultrafiltration, obtains the Herba Epimedii activity extract that molecular weight ranges are 5000Da-6000Da, stand-by；

C, the water of 2-5 times of volume of moringa seeds addition is smashed to pieces, and the high-voltage pulse electric that electric field strength is 25KV/cm is used after smashing into slurry to pieces Field processing, the time of pulse is 5 × 10^-6-10×10^-6S, the frequency of pulse is 30-50 hertz, and 5-10 is added after being handled 70-80% (v/v) ethanol solution again carries out ultrasonic extraction 10-20 minutes using Ultrasound Instrument, and the current strength of Ultrasound Instrument is 10A/25W-15A/25W, then pours into 0.5 μm of ceramic film component with the flow velocity of 0.1-0.25m/s and carries out micro-filtration, in 0.1- Under 0.2MPa pressure differentials, successively with the membrane module of molecular cut off 5000000Da, 1O000Da, it is super that circulation is carried out with cross-current flow Filter is handled, and obtains the moringa seeds activity extract that molecular weight ranges are 10000Da-15000Da, stand-by；

D, fresh olive leaf is ground into and the water progress water of 3-5 times of material quality is added after slurry carries 10-20 minutes, then mistake Filter obtains thick liquid, and thick liquid is poured into polyamide chromatography column with the flow velocity of 0.1-0.3m/s often chromatographs, and uses 50-60% (v/v) Ethanol solution carries out 3 elutions, and it is 60-70 that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution, DEG C, thickening filtration is then carried out, olive leaf activity extract is obtained after being concentrated under reduced pressure, it is stand-by；

E, purslane herb being cleaned after the picking of morning 8-9 points and cleaned, addition quality carries out water for the water of 3-5 times of herb and carries, Thick liquid is obtained after filtering, thick liquid is carried out after being concentrated under reduced pressure into 1.01 times of original volume, adds the 65-75% (v/ of 5-10 times of volume V) ethanol solution carries out alcohol extracting 20-30 minutes, takes filtrate to be spray-dried to obtain purslane activity extract after filtering, treats With；

F, lotus seeds are shelled and leaves embryo nelumbinis drying to ultramicro grinding after moisture content 2-5%, extraction is added after weighing the powder of 1kg In tank, add 65-75% (v/v) ethanol solution and carry out refluxing extraction 3 times, add the ethanol solution of 5 times of powder quality for the first time Refluxing extraction 60 minutes, filters to take filtrate, is subsequently added into the ethanol solution refluxing extraction of 3 times of filter residue quality 50 minutes, filters to take Filtrate, is subsequently added into the ethanol solution refluxing extraction of 2 times of filter residue quality 30 minutes, filters to take filtrate, and filtrate three times is merged, Carry out being concentrated under reduced pressure into 1.01 times of original volume again, be then dried under reduced pressure using pressure Reduction Dryer, crushed 100 mesh sieves, obtained It is stand-by to lotus seeds activity extract；

G, the fresh stem of dendrobium candidum is put into mixer and is stirred crushing, then using 65-75% (v/v) ethanol solution in temperature Stayed overnight to carry out alcohol extracting at -5--3 DEG C, use rotating speed that filtering is centrifuged for the centrifuge of 200-500r/min after overnight Thick liquid is obtained, when room temperature extraction 1-3 is small after thick liquid is mixed with protease, dendrobium candidum activity extract is obtained by filtration, it is stand-by, The addition of protease accounts for the 0.05-0.1% of thick liquid gross mass；

H, blended after cacumen biotae is cleaned using mixer, be subsequently added into 5-10 times of water and carry out refluxing extraction 3 times, often When secondary 1-2 is small, filtering filtrate will merge three times, it is dry be concentrated into 1.01 times of original volume after, it is molten to add 70-80% (v/v) ethanol Liquid carried out at 5-8 DEG C alcohol extracting 5-8 it is small when, centrifugation take precipitation spray drying obtain cacumen biotae activity extract, it is stand-by；

I, obtained after honey-fried licorice root stem tuber is crushed add 70-80% (v/v) ethanol solution carry out circulation extraction extracted Liquid, is 2.0-3.0 by extracting solution addition salt acid for adjusting pH, is then stirred while heating water bath in extracting solution until silk occur Shape thing starts timing and continues stirring 5-10 minutes, filters to take filtrate in time, filter residue repeats said extracted step 2 time, by the filter of 3 times Liquid merge be dried under reduced pressure, crush after can obtain honey-fried licorice root activity extract, it is stand-by；

J, after the flower of witloof is dried, 70-80% (v/v) ethanol solution for adding 5-10 times of volume uses apparatus,Soxhlet's When progress surname extraction 2-3 is small afterwards, stirred after filtering to take the chitosan solution for the 0.01-0.02% that filtrate adds filtrate gross mass Until filtrate is clarified, filter off filtrate and carry out heating concentration, be freeze-dried to obtain witloof activity extract, it is stand-by；

(2) preparation of basestocks：According to percent by weight by cashew nut, fish, rice residue, oviposition row essence after frog ovary, Wheat bran, coix seed, defatted soybean add water after being crushed, and when progress boiling 10-20 is small under pressure is 0.1-0.2Mpa, connect The celery smashed to pieces will be admixed by, and continue, when progress boiling 10-20 is small under pressure is 0.2-0.3Mpa, to obtain base after mixing Plinth material；

(3) preparation of health care fish sauce：By the stand-by material mixing in step (1), (2) and add brine, soy sauce koji mixing addition hair In ferment cylinder, closing fermentation 10-15 days at 25-30 DEG C, then shine cylinder 30-40 days under the sun, then control temperature to be maintained at Fermentation is to full 8-10 months at 30 DEG C, then added after leaching, filtering, sterilizing citric acid blend to concentration be 28-30 mother-in-law U.S. degree, Precipitation can obtain health care fish sauce.
A kind of 4. preparation method of health care fish sauce according to claim 3, it is characterised in that：Brine described in step (3) Concentration be 15-20 mother-in-law U.S. degree.
A kind of 5. preparation method of health care fish sauce according to claim 3, it is characterised in that：The surrounding environment during fermentation Humidity remain 30-40%.
A kind of 6. preparation method of health care fish sauce according to claim 3, it is characterised in that：Cyanide in the embryo nelumbinis Content is 2.11-2.67mg/Kg.
A kind of 7. preparation method of health care fish sauce according to claim 3, it is characterised in that：The purslane herb it is thick Polyoses content is 10-15mg/g.