CN107881109B - Method for preparing fecal strain liquid by removing copper, lead and zinc ions in human feces through lithium illite - Google Patents
Method for preparing fecal strain liquid by removing copper, lead and zinc ions in human feces through lithium illite Download PDFInfo
- Publication number
- CN107881109B CN107881109B CN201711214878.5A CN201711214878A CN107881109B CN 107881109 B CN107881109 B CN 107881109B CN 201711214878 A CN201711214878 A CN 201711214878A CN 107881109 B CN107881109 B CN 107881109B
- Authority
- CN
- China
- Prior art keywords
- illite
- excrement
- lithium
- lead
- slurry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000002550 fecal effect Effects 0.000 title claims abstract description 54
- 229910052900 illite Inorganic materials 0.000 title claims abstract description 41
- VGIBGUSAECPPNB-UHFFFAOYSA-L nonaaluminum;magnesium;tripotassium;1,3-dioxido-2,4,5-trioxa-1,3-disilabicyclo[1.1.1]pentane;iron(2+);oxygen(2-);fluoride;hydroxide Chemical compound [OH-].[O-2].[O-2].[O-2].[O-2].[O-2].[F-].[Mg+2].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[K+].[K+].[K+].[Fe+2].O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2.O1[Si]2([O-])O[Si]1([O-])O2 VGIBGUSAECPPNB-UHFFFAOYSA-L 0.000 title claims abstract description 41
- 229910052802 copper Inorganic materials 0.000 title claims abstract description 38
- 239000010949 copper Substances 0.000 title claims abstract description 38
- 239000011133 lead Substances 0.000 title claims abstract description 38
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 title claims abstract description 37
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 31
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 229910052744 lithium Inorganic materials 0.000 title claims abstract description 27
- 210000003608 fece Anatomy 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 title claims description 33
- 239000002002 slurry Substances 0.000 claims description 44
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 29
- 238000001914 filtration Methods 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 19
- 241000894006 Bacteria Species 0.000 claims description 18
- 239000002245 particle Substances 0.000 claims description 16
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- 239000011780 sodium chloride Substances 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 9
- MRVHOJHOBHYHQL-UHFFFAOYSA-M lithium metaphosphate Chemical compound [Li+].[O-]P(=O)=O MRVHOJHOBHYHQL-UHFFFAOYSA-M 0.000 claims description 9
- 230000009965 odorless effect Effects 0.000 claims description 9
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 6
- 238000005341 cation exchange Methods 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 6
- 239000011707 mineral Substances 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 5
- 239000001569 carbon dioxide Substances 0.000 claims description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 4
- 229910052786 argon Inorganic materials 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000001307 helium Substances 0.000 claims description 3
- 229910052734 helium Inorganic materials 0.000 claims description 3
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 claims description 3
- 229910052754 neon Inorganic materials 0.000 claims description 3
- GKAOGPIIYCISHV-UHFFFAOYSA-N neon atom Chemical compound [Ne] GKAOGPIIYCISHV-UHFFFAOYSA-N 0.000 claims description 3
- 239000010801 sewage sludge Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 7
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 238000002054 transplantation Methods 0.000 description 8
- 229910001220 stainless steel Inorganic materials 0.000 description 7
- 239000010935 stainless steel Substances 0.000 description 7
- 210000001035 gastrointestinal tract Anatomy 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 6
- 229910001415 sodium ion Inorganic materials 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 239000010794 food waste Substances 0.000 description 3
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 3
- 229910052808 lithium carbonate Inorganic materials 0.000 description 3
- 239000013065 commercial product Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000009616 inductively coupled plasma Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 208000037384 Clostridium Infections Diseases 0.000 description 1
- 206010009657 Clostridium difficile colitis Diseases 0.000 description 1
- 206010054236 Clostridium difficile infection Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000792859 Enema Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000002052 colonoscopy Methods 0.000 description 1
- 208000016097 disease of metabolism Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007920 enema Substances 0.000 description 1
- 229940095399 enema Drugs 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 229910052745 lead Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009044 synergistic interaction Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910000166 zirconium phosphate Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
- B01J20/12—Naturally occurring clays or bleaching earth
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
- Geochemistry & Mineralogy (AREA)
- Inorganic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing a fecal bacterial solution by removing copper, lead and zinc ions in human feces by utilizing lithium illite. The invention can remove copper, lead and zinc ions in human excrement by specific adsorption, so that excrement donated by excrement donors with excessive copper, lead and zinc ions can reach the standard after treatment.
Description
Technical Field
The invention belongs to the technical field of human fecal bacteria transplantation, fermentation and detection, and relates to a method for removing copper, lead and zinc ions in human feces by utilizing lithium illite.
Background
"Fecal transplantation" (FMT) refers to the transplantation of functional flora in the feces of healthy people into the gastrointestinal tract of patients to reconstruct new intestinal flora and realize the treatment of intestinal and parenteral diseases. Fecal transplantation has been used for the treatment and exploratory studies of various flora-related diseases such as clostridium difficile infection, inflammatory bowel disease, intractable constipation, metabolic disease, intestinal immunodeficiency, intestinal allergy and the like as an effective means for reconstructing intestinal flora, and is considered as a breakthrough medical progress in recent years.
The fecal bacteria feeding way is divided into an upper digestive tract, a middle digestive tract and a lower digestive tract. The upper digestive tract route mainly refers to oral fecal bacteria capsule; the middle digestive tract route comprises passing through a nasointestinal tube, a gastroscope forceps channel hole and a percutaneous endoscopic gastrostomy jejunum tube; the lower gastrointestinal route includes colonoscopy, enema, colostomy, and endoscopic intestinal catheterization (TET). The physician should select the most appropriate input route for the patient according to his condition and the characteristics of the various routes.
The state of the coprophilous fungi transplantation can be divided into three forms of freshness, freezing storage and fermentation. Since the safety of fecal bacteria transplantation is the primary consideration in clinical decision making, in order to isolate a high quality fecal flora without pathogens, the fecal donors are highly demanding, at least within 3-6 months no diarrhea occurs, no antibiotics have been used, and there must be no infectious diseases and gastrointestinal diseases, in addition, there are limitations including age, physiology, pathology, psychology, integrity, time, environment, etc.
As the fecal bacteria provided by qualified fecal donors can not meet the clinical requirements at all, the fecal bacteria with certain ions exceeding the standard is processed by the technology, so that the fecal bacteria meets the requirements of fecal bacteria transplantation, and the method has very important practical significance.
Along with the change of human living environment, the exceeding of copper, lead and zinc ions in human excrement is more and more common, and when the copper, lead and zinc ions in the excrement of an excrement provider exceed the standard, the requirement of excrement bacterium transplantation is not met.
Disclosure of Invention
The invention aims to provide a method for removing copper, lead and zinc ions in human excrement by utilizing lithium illite.
The technical scheme adopted by the invention is as follows:
a method for preparing a fecal bacteria liquid by removing copper, lead and zinc ions in human feces by utilizing lithium illite,
1) adding a sterile NaCl solution into the excrement sample, adding Likelite, and uniformly stirring to obtain excrement slurry;
2) primarily filtering the excrement slurry, taking filtrate, and stirring the filtrate at 1800-2200 rpm for 3-6 min every 0.8-1.2 h under the protection of carbon dioxide, nitrogen, helium, neon or argon, and obtaining the excrement and bacterium slurry after 4-7 h;
3) filtering the fecal strain slurry to obtain filtrate to obtain crude fecal strain liquid; and centrifuging the crude fecal strain liquid, taking the precipitate, and re-suspending to obtain colorless and odorless fecal strain liquid containing no copper, lead and zinc ions.
Further, the preparation method of the lithium illite comprises the following steps: adding water and lithium metaphosphate into illite ore, heating to 70-80 ℃, and adding Na2CO3(ii) a Stirring for 2.5-3.5 h, standing for 20-30 h, then centrifugally washing with deionized water, recovering solids, and drying to obtain the Lidikite.
Further, the illite mineral, lithium metaphosphate and Na2CO3And the dosage ratio of water is 80-120 g: 8-12 g: 18-22 g: 1L of the compound.
Furthermore, the cation exchange capacity of the obtained lithium-based illite is 40-60 mmol/100 g.
Further, in the step 1), the dosage ratio of the excrement sample, the NaCl solution and the Lidikite is 1 g: 3-7 mL: 0.5 to 1.5 g.
Further, the concentration of the NaCl solution is 0.85-0.95% w/v.
Further, in the step 1), the stirring speed is 1800-2200 rpm, and the time is 2-4 min.
Further, in step 2), the specific operations of the primary filtering are as follows: and filtering the excrement slurry by using a filter screen with the aperture of 4.5-5.5 mm to remove large-particle substances.
Further, in step 3), the specific operation of filtering the fecal sewage sludge is as follows: filtering by a filter screen with the aperture of 1.5-2.5 mm, 0.8-1.2 mm, 0.4-0.6 mm and 0.2-0.3 mm in sequence.
Further, in the step 3), the rotation speed of the centrifugation is 5500-6500 rpm, and the time is 12-18 min.
The invention has the beneficial effects that:
at present, a method for removing copper, lead and zinc ions in human excrement is not reported temporarily, and the method can remove the copper, lead and zinc ions in the human excrement through specific adsorption, so that excrement donated by excrement donors with excessive copper, lead and zinc ions can reach the standard after treatment.
Detailed Description
A method for preparing a fecal bacteria liquid by removing copper, lead and zinc ions in human feces by utilizing lithium illite,
1) adding a sterile NaCl solution into the excrement sample, adding Likelite, and uniformly stirring to obtain excrement slurry;
2) primarily filtering the excrement slurry, taking filtrate, and stirring the filtrate at 1800-2200 rpm for 3-6 min every 0.8-1.2 h under the protection of carbon dioxide, nitrogen, helium, neon or argon, and obtaining the excrement and bacterium slurry after 4-7 h;
3) filtering the fecal strain slurry to obtain filtrate to obtain crude fecal strain liquid; and centrifuging the crude fecal strain liquid, taking the precipitate, and re-suspending to obtain colorless and odorless fecal strain liquid containing no copper, lead and zinc ions.
Preferably, the preparation method of the lithium illite comprises the following steps: adding water and lithium metaphosphate into illite ore, heating to 70-80 ℃, and adding Na2CO3(ii) a Stirring for 2.5-3.5 h, standing for 20-30 h, then centrifugally washing with deionized water, recovering solids, and drying to obtain the Lidikite.
Preferably, the illite mineral, lithium metaphosphate and Na2CO3And the dosage ratio of water is 80-120 g: 8-12 g: 18-22 g: 1L of the compound.
Preferably, the drying temperature is 100-110 ℃, and the drying time is 45-55 h.
Preferably, the cation exchange capacity of the obtained lithium illite is 40-60 mmol/100 g.
Preferably, in the step 1), the dosage ratio of the excrement sample to the NaCl solution to the lithium illite is 1 g: 3-7 mL: 0.5 to 1.5 g.
Preferably, the concentration of the NaCl solution is 0.85-0.95% w/v.
Preferably, in the step 1), the stirring speed is 1800-2200 rpm, and the time is 2-4 min.
Preferably, in step 2), the preliminary filtration specifically comprises: and filtering the excrement slurry by using a filter screen with the aperture of 4.5-5.5 mm to remove large-particle substances.
Preferably, in step 3), the specific operation of filtering the fecal sewage sludge is as follows: filtering by a filter screen with the aperture of 1.5-2.5 mm, 0.8-1.2 mm, 0.4-0.6 mm and 0.2-0.3 mm in sequence.
Preferably, in the step 3), the rotation speed of the centrifugation is 5500-6500 rpm, and the time is 12-18 min.
Preferably, in step 3), the liquid used for resuspension is a sterile 0.85% -0.95% w/v NaCl solution.
The present invention will be further described with reference to the following examples.
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
It should be understood that the processing equipment or devices not specifically mentioned in the following examples are conventional in the art; all pressure values and ranges refer to absolute pressures.
Furthermore, it is to be understood that one or more method steps mentioned in the present invention does not exclude that other method steps may also be present before or after the combined steps or that other method steps may also be inserted between these explicitly mentioned steps, unless otherwise indicated; it is also to be understood that a combined connection between one or more devices/apparatus as referred to in the present application does not exclude that further devices/apparatus may be present before or after the combined device/apparatus or that further devices/apparatus may be interposed between two devices/apparatus explicitly referred to, unless otherwise indicated. Moreover, unless otherwise indicated, the numbering of the various method steps is merely a convenient tool for identifying the various method steps, and is not intended to limit the order in which the method steps are arranged or the scope of the invention in which the invention may be practiced, and changes or modifications in the relative relationship may be made without substantially changing the technical content.
Wherein the concentration of Cu, Pb and Zn ions in fecal bacteria is measured by inductively coupled plasma spectrometer (ICP, Optima 2000DV, USA) (test methods reference Xiaoang Cai, Guang-Jian Dai, Shao-Zao Tan, Yu Ouyang, You-Sheng Ouyang, Qi-Shan Shi. synergistic interaction information and ceramic ions loaded. alpha. -zirconium phosphate. materials letters.2012,67(1): 199-201.).
Example 1 method for preparing fecal bacterial liquid by removing copper, lead and zinc ions in human feces with lithium illite
(1) Preparation of lithium illite: adding 100g illite mineral (commercial product, particle size of 0.5-2.0 mm) into 1L water, adding 10g lithium metaphosphate, heating to 75 deg.C, adding 20g Na2CO3(ii) a Stirring for 3h at an electromagnetic speed of 60rpm, standing for 24h, centrifugally washing for 4 times by using deionized water, removing insoluble substances at the bottom, and recovering solids; drying at 105 ℃ for 48h to obtain lithium illite; the Cation Exchange Capacity (CEC) of the resulting Liulilite was 50mmol/100 g.
(2) Taking 30 +/-0.1 g of lithium illite (the particle size is 0.5-2.0 mm), adding 100mL of sterile 0.9% NaCl solution, stirring at 500r/min for 5min, and filtering out water by a stainless steel filter screen of 0.25mm to obtain lithium illite slurry for later use.
(3) Placing 50 +/-1 g of donor fresh excrement in a stirrer, adding 250mL of sterile 0.9% NaCl solution, adding the prepared lithium illite slurry (the particle size is 0.5-2.0 mm), and homogenizing at 2000r/min for 3min to obtain excrement slurry;
(4) filtering the excrement slurry by a stainless steel filter screen with the thickness of 5.0mm to remove large-particle substances in the excrement slurry, and obtaining excrement slurry after primary filtration;
(5) transporting the primarily filtered excrement slurry ice to a laboratory and processing the excrement slurry ice within 2 hours after collection;
(6) transferring the primarily filtered excrement slurry into a nitrogen bioengineering kitchen, introducing carbon dioxide gas, and stirring for 5min at a speed of 2000r/min in a homogenizer every 1h, and obtaining excrement and bacteria slurry after 6 h;
(7) filtering the fecal strain slurry by stainless steel filter screens with diameters of 2.0mm, 1.0mm, 0.5mm and 0.25mm step by step to remove Li-based illite, lithium carbonate, unabsorbed food residues and small particle substances which adsorb copper, lead and zinc ions, and obtaining coarse fecal strain liquid;
(8) the crude fecal bacterial liquid is centrifuged at 6000r/min for 15min, and the fecal bacterial pellet is resuspended in 250mL of 0.9% NaCl solution to obtain 250mL of nearly colorless and odorless fecal bacterial liquid.
The total content of copper, lead and zinc ions in the obtained fecal strain liquid is determined to be zero (the total content of copper, lead and zinc ions is less than 4ppb, which cannot be detected by an instrument), and the content of sodium ions is 154 mmol/L.
Blank control group: the procedure was the same as in the above-mentioned steps (1) to (8) of this example, except that 250mL of a nearly colorless and odorless liquid was obtained without adding feces and Liulite, and the total content of copper, lead and zinc ions in the liquid was determined to be zero (no instrument detection, total content of copper, lead and zinc ions < 4ppb), and sodium ion content was determined to be zero (see Table 1).
Comparative group 1 (with feces and without illite): the procedure was as in the above-mentioned steps (1) to (8) of this example except that no Lidikite was added to obtain 250mL of a nearly colorless and odorless liquid, and the contents of copper, lead and zinc ions in the liquid were measured as 1.3mmol/L, 3.1mmol/L and 1.6mmol/L, respectively, and the total content of copper, lead and zinc ions was measured as 6mmol/L and the content of sodium ions was measured as 154 mmol/L.
Comparative group 2 (illite with stool): the procedure was as in the above-mentioned steps (1) to (8) of this example except that no Likite was added to obtain 250mL of a nearly colorless and odorless liquid, and the contents of copper, lead and zinc ions in the liquid were measured to be 1.1mmol/L, 2.7mmol/L and 1.4mmol/L, respectively, and the total content of copper, lead and zinc ions was measured to be 5.2mmol/L and the content of sodium ions was measured to be 156.1mmol/L (see Table 1).
TABLE 1 results of the determination of the ion content in the different treatment groups
Note:ammol/L, mmol/L;
bN/A, which cannot be detected by instruments, and the content is less than 4ppb (ppb: mu g/L).
As can be seen from Table 1, the donor fresh feces treated by Lidikite and having excessive copper, lead and zinc ions no longer contain copper, lead and zinc ions, so that the feces donated by feces donors having excessive copper, lead and zinc ions can reach the standard after treatment, and the content of sodium ions is basically kept unchanged.
Embodiment 2 method for preparing fecal bacterial liquid by removing copper, lead and zinc ions in human feces through lithium illite
(1) Preparation of lithium illite: adding 120g of illite mineral (commercial product, particle size of 0.5-2.0 mm) into 1L of water, adding 8g of lithium metaphosphate, heating to 80 ℃, and adding 22g of Na2CO3(ii) a Stirring at an electromagnetic speed of 60rpm for 3.5h, standing for 20h, centrifuging and washing with deionized water for 4 times, removing insoluble substances at the bottom, and recovering solids; drying for 45h at 100 ℃ to obtain the Liulite; the Cation Exchange Capacity (CEC) of the resulting Liulilite was 60mmol/100 g.
(2) Placing 50 +/-1 g of donor fresh excrement in a stirrer, adding 150mL of sterile 0.95% NaCl solution, adding 25g of lithium-based illite (the particle size is 0.5-2.0 mm), and homogenizing at 2200r/min for 4min to obtain excrement slurry;
(3) filtering the excrement slurry by a stainless steel filter screen with the thickness of 5.0mm to remove large-particle substances in the excrement slurry, and obtaining excrement slurry after primary filtration;
(4) transporting the primarily filtered excrement slurry ice to a laboratory and processing the excrement slurry ice within 2 hours after collection;
(5) transferring the primarily filtered excrement slurry into a nitrogen bioengineering kitchen, introducing nitrogen gas, and stirring for 5min at a speed of 2000r/min in a homogenizer every 1.2h, and obtaining excrement and bacteria slurry after 4 h;
(6) filtering the fecal strain slurry by stainless steel filter screens with diameters of 2.0mm, 1.0mm, 0.5mm and 0.25mm step by step to remove Li-based illite, lithium carbonate, unabsorbed food residues and small particle substances which adsorb copper, lead and zinc ions, and obtaining coarse fecal strain liquid;
(7) the crude fecal strain liquid is centrifuged at 6500r/min for 18min, and the fecal strain precipitate is resuspended in 250mL of 0.9% NaCl solution to obtain 250mL of nearly colorless and odorless fecal strain liquid free of copper, lead and zinc ions.
Example 3 method for preparing fecal bacterial liquid by removing copper, lead and zinc ions in human feces with lithium illite
(1) Preparation of lithium illite: 80g of illite mineral (commercially available product, particle size 0.5-2.0 mm) was added to the mixtureAdding 12g of lithium metaphosphate into 1L of water, heating to 70 ℃, and adding 18g of Na2CO3(ii) a Stirring for 2.5h at an electromagnetic speed of 60rpm, standing for 30h, centrifuging and washing for 4 times by using deionized water, removing insoluble substances at the bottom, and recovering solids; drying at 110 ℃ for 55h to obtain the Lidikite; the Cation Exchange Capacity (CEC) of the resulting Liulilite was 50mmol/100 g.
(2) Placing 50 +/-1 g of donor fresh excrement in a stirrer, adding 350mL of sterile 0.85% NaCl solution, adding 75g of lithium illite (the particle size is 0.5-2.0 mm), and homogenizing at 2200r/min for 4min to obtain excrement slurry;
(3) filtering the excrement slurry by a stainless steel filter screen with the thickness of 5.0mm to remove large-particle substances in the excrement slurry, and obtaining excrement slurry after primary filtration;
(4) transporting the primarily filtered excrement slurry ice to a laboratory and processing the excrement slurry ice within 2 hours after collection;
(5) transferring the primarily filtered excrement slurry into a nitrogen bioengineering kitchen, introducing nitrogen gas, and stirring for 5min at a speed of 2000r/min in a homogenizer every 1.2h, and obtaining excrement and bacteria slurry after 4 h;
(6) filtering the fecal strain slurry by stainless steel filter screens with diameters of 2.0mm, 1.0mm, 0.5mm and 0.25mm step by step to remove Li-based illite, lithium carbonate, unabsorbed food residues and small particle substances which adsorb copper, lead and zinc ions, and obtaining coarse fecal strain liquid;
(7) the crude fecal strain liquid is centrifuged at 6500r/min for 12min, and the fecal strain precipitate is resuspended in 250mL of 0.9% NaCl solution to obtain 250mL of nearly colorless and odorless fecal strain liquid free of copper, lead and zinc ions.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A method for preparing a fecal strain solution by removing copper, lead and zinc ions in human feces by utilizing lithium illite is characterized by comprising the following steps:
1) adding a sterile NaCl solution into the excrement sample, adding Likelite, and uniformly stirring to obtain excrement slurry;
2) primarily filtering the excrement slurry, taking filtrate, and stirring the filtrate at 1800-2200 rpm for 3-6 min every 0.8-1.2 h under the protection of carbon dioxide, nitrogen, helium, neon or argon, and obtaining the excrement and bacterium slurry after 4-7 h;
3) filtering the fecal strain slurry to obtain filtrate to obtain crude fecal strain liquid; centrifuging the crude fecal strain liquid, taking the precipitate, and re-suspending to obtain colorless and odorless fecal strain liquid containing no copper, lead and zinc ions;
the preparation method of the lithium illite comprises the following steps: adding water and lithium metaphosphate into illite ore, heating to 70-80 ℃, and adding Na2CO3(ii) a Stirring for 2.5-3.5 h, standing for 20-30 h, then centrifugally washing with deionized water, recovering solids, and drying to obtain the Lidikite.
2. The method of claim 1, wherein the illite mineral, lithium metaphosphate, Na2CO3And the dosage ratio of water is 80-120 g: 8-12 g: 18-22 g: 1L of the compound.
3. The method according to claim 1, wherein the cation exchange capacity of the obtained lithium illite is 40 to 60mmol/100 g.
4. The method according to claim 1, wherein in the step 1), the dosage ratio of the excrement sample, the NaCl solution and the Liulite is 1 g: 3-7 mL: 0.5 to 1.5 g.
5. The method according to claim 1, wherein the concentration of the NaCl solution is between 0.85% and 0.95% w/v.
6. The method according to claim 1, wherein in the step 1), the stirring speed is 1800-2200 rpm and the time is 2-4 min.
7. The method according to claim 1, wherein in step 2), the preliminary filtering is performed by: and filtering the excrement slurry by using a filter screen with the aperture of 4.5-5.5 mm to remove large-particle substances.
8. The method according to claim 1, wherein in the step 3), the specific operation of the fecal sewage sludge filtration is as follows: filtering by a filter screen with the aperture of 1.5-2.5 mm, 0.8-1.2 mm, 0.4-0.6 mm and 0.2-0.3 mm in sequence.
9. The method according to claim 1, wherein in the step 3), the rotation speed of the centrifugation is 5500-6500 rpm, and the time is 12-18 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711214878.5A CN107881109B (en) | 2017-11-28 | 2017-11-28 | Method for preparing fecal strain liquid by removing copper, lead and zinc ions in human feces through lithium illite |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711214878.5A CN107881109B (en) | 2017-11-28 | 2017-11-28 | Method for preparing fecal strain liquid by removing copper, lead and zinc ions in human feces through lithium illite |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107881109A CN107881109A (en) | 2018-04-06 |
| CN107881109B true CN107881109B (en) | 2020-11-17 |
Family
ID=61775726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711214878.5A Active CN107881109B (en) | 2017-11-28 | 2017-11-28 | Method for preparing fecal strain liquid by removing copper, lead and zinc ions in human feces through lithium illite |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107881109B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4687521A (en) * | 1984-03-02 | 1987-08-18 | National Research Development Corporation | Randomly interstratified clays |
| CN104922158A (en) * | 2015-06-05 | 2015-09-23 | 中国人民解放军第三军医大学第三附属医院 | Fecal microbiota capsule, as well as preparation method and application thereof |
| CN104984736A (en) * | 2015-06-23 | 2015-10-21 | 中南大学 | Blood heavy metal ion adsorbent, preparation method thereof and blood perfusion device |
| CN106946485A (en) * | 2017-03-24 | 2017-07-14 | 吉林大学 | A kind of lithium is modified illite cement intensifier and preparation method |
-
2017
- 2017-11-28 CN CN201711214878.5A patent/CN107881109B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4687521A (en) * | 1984-03-02 | 1987-08-18 | National Research Development Corporation | Randomly interstratified clays |
| CN104922158A (en) * | 2015-06-05 | 2015-09-23 | 中国人民解放军第三军医大学第三附属医院 | Fecal microbiota capsule, as well as preparation method and application thereof |
| CN104984736A (en) * | 2015-06-23 | 2015-10-21 | 中南大学 | Blood heavy metal ion adsorbent, preparation method thereof and blood perfusion device |
| CN106946485A (en) * | 2017-03-24 | 2017-07-14 | 吉林大学 | A kind of lithium is modified illite cement intensifier and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 蒙脱石、高岭石、伊利石对重金属离子吸附容量的实验研究;何宏平等;《岩石矿物学杂志》;20040108;第20卷(第4期);第2页第6段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107881109A (en) | 2018-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2986915C (en) | Microbiota restoration therapy (mrt) compositions and methods of manufacture | |
| CN104922158A (en) | Fecal microbiota capsule, as well as preparation method and application thereof | |
| CN112089736B (en) | Whole fungus capsule and preparation method and application thereof | |
| CN109355349B (en) | Ackermansoni specificity screening culture medium and preparation method and application thereof | |
| CN109010380A (en) | A kind of technique that caprophyl transplants the intestinal flora preparation in clinical treatment | |
| CN109394795A (en) | A kind of intestines bacterium capsule preparation method thereof and intestines bacterium capsule | |
| CN107881109B (en) | Method for preparing fecal strain liquid by removing copper, lead and zinc ions in human feces through lithium illite | |
| CN109628311A (en) | Freeze drying protectant, freeze-dried powder, capsule and preparation method thereof for protecting enterobacteriaceae | |
| CN108085270B (en) | Method for preparing iron-free titanium bismuth ion-free fecal bacteria liquid | |
| CN108048325B (en) | Method for removing barium, gallium and manganese ions in human excrement by using lithium-based halloysite | |
| CN107974410B (en) | Method for removing magnesium, calcium and aluminum ions in human excrement by using lithium-based vermiculite | |
| CN108085271B (en) | Method for removing mercury, thallium and arsenic ions in human excrement | |
| CN108152096B (en) | Method for removing tellurium, selenium and cobalt ions in human excrement by utilizing lithium-based chlorite | |
| CN106176821A (en) | A kind of Tiny ecosystem vagina microbial inoculum and its preparation method and application | |
| Rivas et al. | Approach to kidney stones associated with ureteropelvic junction obstruction during laparoscopic pyeloplasty | |
| CN112336753A (en) | A kind of intestinal flora capsule and its preparation method and application | |
| CN110938563B (en) | Lactobacillus BJ-REBORN001 and application thereof in preparation of helicobacter pylori inhibiting fermentation broth | |
| CN107034159B (en) | Pathogenic bacteria antagonist and application thereof | |
| Gupta et al. | Fecal Transplant Technolo¬ gy: An Effective Therapeutic Method for Many Diseases | |
| CN109700830A (en) | A kind for the treatment of gastritis and the drug of peptic ulcer in good taste and preparation method thereof | |
| CN110742929A (en) | Method for preparing coprophila transplantation capsule containing benzoylaconitine by fermenting monkshood with coprophila | |
| CN109757730B (en) | Composition with functions of reducing blood fat, blood pressure and blood sugar and preparation method thereof | |
| CN111534466B (en) | A kind of processing method and processing reagent of human fecal intestinal flora sample | |
| CN116694492A (en) | Helicobacter pylori enrichment medium, preparation method thereof and helicobacter pylori enrichment method | |
| CN112656766A (en) | Fecal fungus freeze-drying and capsule preparation process for fecal fungus transplantation treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |