CN107875454A - A kind of collagen composite membrane for guide tissue regeneration and preparation method and application - Google Patents
A kind of collagen composite membrane for guide tissue regeneration and preparation method and application Download PDFInfo
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- CN107875454A CN107875454A CN201711208637.XA CN201711208637A CN107875454A CN 107875454 A CN107875454 A CN 107875454A CN 201711208637 A CN201711208637 A CN 201711208637A CN 107875454 A CN107875454 A CN 107875454A
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- composite membrane
- collagen composite
- tissue regeneration
- sis
- matter powder
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- 239000012528 membrane Substances 0.000 title claims abstract description 63
- 102000008186 Collagen Human genes 0.000 title claims abstract description 60
- 108010035532 Collagen Proteins 0.000 title claims abstract description 60
- 229920001436 collagen Polymers 0.000 title claims abstract description 60
- 239000002131 composite material Substances 0.000 title claims abstract description 58
- 230000017423 tissue regeneration Effects 0.000 title claims abstract description 55
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000463 material Substances 0.000 claims abstract description 93
- 239000000843 powder Substances 0.000 claims abstract description 74
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 70
- 239000011707 mineral Substances 0.000 claims abstract description 70
- 230000000975 bioactive effect Effects 0.000 claims abstract description 66
- 239000010409 thin film Substances 0.000 claims abstract description 53
- 239000010408 film Substances 0.000 claims description 38
- 239000000203 mixture Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 14
- 239000000017 hydrogel Substances 0.000 claims description 13
- 238000011069 regeneration method Methods 0.000 claims description 13
- 230000008929 regeneration Effects 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 11
- 239000000499 gel Substances 0.000 claims description 11
- DLYUQMMRRRQYAE-UHFFFAOYSA-N phosphorus pentoxide Inorganic materials O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 claims description 9
- 239000000377 silicon dioxide Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 229920001661 Chitosan Polymers 0.000 claims description 8
- KKCBUQHMOMHUOY-UHFFFAOYSA-N Na2O Inorganic materials [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 229910052681 coesite Inorganic materials 0.000 claims description 7
- 229910052906 cristobalite Inorganic materials 0.000 claims description 7
- 239000002105 nanoparticle Substances 0.000 claims description 7
- 229910052682 stishovite Inorganic materials 0.000 claims description 7
- 229910052905 tridymite Inorganic materials 0.000 claims description 7
- 229920000656 polylysine Polymers 0.000 claims description 6
- 108010039918 Polylysine Proteins 0.000 claims description 5
- 229920001577 copolymer Polymers 0.000 claims description 5
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 3
- 229920002674 hyaluronan Polymers 0.000 claims description 3
- 229960003160 hyaluronic acid Drugs 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 2
- 239000008273 gelatin Substances 0.000 claims description 2
- 229920000159 gelatin Polymers 0.000 claims description 2
- 235000019322 gelatine Nutrition 0.000 claims description 2
- 235000011852 gelatine desserts Nutrition 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims 1
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 2
- 210000004379 membrane Anatomy 0.000 description 44
- 210000000988 bone and bone Anatomy 0.000 description 13
- 230000007547 defect Effects 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 8
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- 238000006731 degradation reaction Methods 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 239000000292 calcium oxide Substances 0.000 description 5
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 5
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- 150000001875 compounds Chemical class 0.000 description 4
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- 239000000047 product Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000003431 cross linking reagent Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000007943 implant Substances 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 230000003239 periodontal effect Effects 0.000 description 3
- 210000000813 small intestine Anatomy 0.000 description 3
- 210000004872 soft tissue Anatomy 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000000956 alloy Substances 0.000 description 2
- 210000001909 alveolar process Anatomy 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000005238 degreasing Methods 0.000 description 2
- YWEUIGNSBFLMFL-UHFFFAOYSA-N diphosphonate Chemical compound O=P(=O)OP(=O)=O YWEUIGNSBFLMFL-UHFFFAOYSA-N 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000005342 ion exchange Methods 0.000 description 2
- 239000003607 modifier Substances 0.000 description 2
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- 244000052769 pathogen Species 0.000 description 2
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- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 241000606750 Actinobacillus Species 0.000 description 1
- 208000010266 Aggressive Periodontitis Diseases 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229920000954 Polyglycolide Polymers 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241000605862 Porphyromonas gingivalis Species 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- 241001135221 Prevotella intermedia Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 208000031650 Surgical Wound Infection Diseases 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 241000589892 Treponema denticola Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 229920003232 aliphatic polyester Polymers 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
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- 229910052586 apatite Inorganic materials 0.000 description 1
- 230000003416 augmentation Effects 0.000 description 1
- 230000003305 autocrine Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000008468 bone growth Effects 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- FYHXNYLLNIKZMR-UHFFFAOYSA-N calcium;carbonic acid Chemical group [Ca].OC(O)=O FYHXNYLLNIKZMR-UHFFFAOYSA-N 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005253 cladding Methods 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
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- 208000014674 injury Diseases 0.000 description 1
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- 238000002955 isolation Methods 0.000 description 1
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- 230000002045 lasting effect Effects 0.000 description 1
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- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001582 osteoblastic effect Effects 0.000 description 1
- 210000004409 osteocyte Anatomy 0.000 description 1
- 230000002138 osteoinductive effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 210000002379 periodontal ligament Anatomy 0.000 description 1
- 201000001245 periodontitis Diseases 0.000 description 1
- 201000006727 periodontosis Diseases 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000004633 polyglycolic acid Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000009418 renovation Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 210000004876 tela submucosa Anatomy 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3629—Intestinal tissue, e.g. small intestinal submucosa
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/112—Phosphorus-containing compounds, e.g. phosphates, phosphonates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/606—Coatings
- A61L2300/608—Coatings having two or more layers
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- Transplantation (AREA)
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- Oral & Maxillofacial Surgery (AREA)
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- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
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- Inorganic Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a kind of collagen composite membrane for guide tissue regeneration and preparation method thereof, the composite membrane is to be evenly applied to be made on SIS thin-film materials by the bioactive minerals matter powder；The collagen composite membrane can be multilayer, including following component：SIS thin-film materials, bioactive minerals matter powder.Collagen composite film-strength prepared by the present invention is high, and histocompatbility is good, also with natural bacteriostatic characteristic, is with a wide range of applications in dental care field.
Description
Technical field
The invention belongs to pharmaceutical technology field, and in particular to a kind of collagen composite membrane and its system for guide tissue regeneration
Preparation Method and application.
Background technology
Guide tissue regeneration (guided tissue regeneration, GTR) is Periodontics research in the late two decades
One of lasting focus.Its general principle is exactly to prevent gingival epithelium root to growth using barrier film, while prevents gum connective
Histocyte contacts with root face, maintains certain space so that periodontal ligament cell occupies root face, and it is newly attached that differentiation and proliferation forms periodontal
.GTR technologies are all proved to be able to obtain different degrees of regeneration in human body and animal body, are opened from the eighties in 19th century
Begin to be applied to treatment periodontitis, achieve preferable clinical effectiveness.
The key of GTR technical research, it is the research to GTR membrane materials.Material applied to GTR technologies must possess barrier
Property and guiding particular organization regeneration function.The guide tissue regeneration film clinically applied can be divided into according to raw material to drop
Solve absorbing film and degradable absorbing film.Non-degradable, which absorbs GTR films, mainly titanium film and expanded polytetrafluoroethylsealing (e-PTFE) film,
It has advantages below:Membrane material is stable, good biocompatibility；There are stronger mechanical strength and space to maintain ability；Can be any
The holdup time in vivo is adjusted, complication occur can remove in time.But its shortcoming is also apparent:Film is not fixed easily；It is easily caused
The appearance of the complication such as mucous membrane splits, film exposure, infection；Need second operation to extract, easily to tissue cause to damage, influence continue into
The effect of bone.
Because non-degradable absorbs the inherent shortcoming of GTR films, biodegradable absorbing material turns into GTR technical research gradually
Important directions.Late 1980s, after biodegradable idea occurs, degradable absorption membrane material is introduced, they incline
To in the second operation for saving taking-up film, treatment time is shortened, ensure that periodontosis tissue regeneration processes are not interrupted, is studied
Scholars think that at least from the perspective of periodontal scholar degradable absorbing film is better than non-degradable absorbing film, and clinical effect
Fruit is suitable with non-degradable absorbing film or more preferable, so turning into guide tissue regeneration film technology using biodegradable absorbing film
A kind of general development trend.
Generally acknowledged preferable degradable absorption GTR films should possess following condition:(1) with good biocompatibility and carefully
Born of the same parents' compatibility；(2) film has certain isolation effect, fibroblast can be prevented to permeate；(3) degradation rate of film is controllable, and with
The regeneration of tissue is mutually coordinated；(4) catabolite has no adverse reaction in vivo, and the degradation process of film does not influence regeneration；(5)
Film has certain mechanical strength, pliability and operability.
Studying more degradable resorbable membrane at present mainly has artificial synthesized high polymer material and the major class of natural material two.
Artificial synthesized high polymer material includes PLA, linear aliphatic polyesters polyvinyl alcohol etc.；Natural macromolecular material include collagen,
Chitosan etc..GTR films prepared by the artificial synthesized high molecular polymer using PLA, polyglycolic acid as representative, in vivo can be with
Degraded absorbs, but can produce slightly sour environment after degraded in vivo, causes inflammatory reaction, is unfavorable for organization healing.Collagen, shell gather
GTR films prepared by the natural polymerses such as sugar, harmful substance will not be produced after degradation in vivo, biocompatibility is better than people
Work synthesized degradable high molecular polymer, but the mechanical performance of the film of natural polymerses preparation and water-resistance are poor, drop
Solve it is too fast, with bone growth speed mismatch.
CN200410071932.1 discloses a kind of double-layer collagen base guide tissue regeneration material and preparation method thereof, is gram
The defects of collagen-based materials degraded is too fast is taken, it improves the anti-degradation capability of material using chemical cross-linking agent, but glutaraldehyde etc. is changed
The toxicity of crosslinking agent is larger, and the elution process after crosslinking is not easy to, and the residual of the crosslinking agent not eluted in the body can be made
The injury of paired human body.
Submucous layer of small intestine (Small Intestinal Submucosa, SIS) is a kind of degradable native biological
Material, there is prominent biological superiority in terms of tissue repair and regeneration.The technology of preparing of SIS materials contains substantially to disappear
The steps such as poison, virus removal, machinery divest, degreasing, de- cell, de-sludging, drying and sterilizing.By rationally treated SIS materials
It is that one kind can be with inducing tissue regeneration, the thin-film material of low immunogenicity.
The content of the invention
It is an object of the invention to provide a kind of collagen composite membrane for guide tissue regeneration and preparation method thereof, the present invention
Collagen composite film-strength is high, and histocompatbility is good, also with natural bacteriostatic characteristic, is had a wide range of applications in dental care field
Prospect.
Technical solution of the present invention is as follows：
A kind of collagen composite membrane for guide tissue regeneration, including following component：SIS thin-film materials, bioactivity ore deposit
Material powder.
Specifically, the bioactive minerals matter powder account for the percentage by weight of the collagen composite membrane for 0.01%~
50%, preferably 1%~30%.
The SIS thin-film materials can be made by existing conventional techniques, such as the method that CN101433735A is recorded.
Usually, the preparation method of the SIS thin-film materials includes via intestinal tube axially cuing open submucous layer of small intestine material
Open, and circumferentially cut into the rectangle SIS units of arbitrary dimension, retain the ring natural width of intestinal tube, then sterilized,
The steps such as degreasing, de- cell, de-sludging processing are made.
Preferably, the bioactive minerals matter powder, the composition containing following percentage by weight：SiO240-65%,
CaO 15-30%, Na2O 15-30%, P2O52-8%；Further, its particle size range is less than 90 μm, wherein, contain aperture
Quasi-nano particle 0.1-20.0wt% in the range of 100-900nm.
Preferably, the bioactive minerals matter powder, the composition containing following percentage by weight：SiO245-61%,
CaO 17-27%, Na2O 19-25%, P2O52.6-6%；Further, its particle size range is less than 90 μm, wherein, contain hole
Quasi-nano particle 2-10% of the footpath in the range of 100-900nm.
It is further preferred that the bioactive minerals matter powder, the composition containing following percentage by weight：SiO2
45%, CaO 24.5%, Na2O 24.5%, P2O56%；Further, its particle size range is less than 90 μm, wherein, contain aperture
Quasi-nano particle 2-5% in the range of 100-900nm.
In above-mentioned bioactive minerals matter powder, the raw material of silica is silica；The raw material of calcium oxide is carbonic acid
Calcium；The raw material of sodium oxide molybdena is sodium carbonate；The raw material of phosphorus pentoxide is phosphorus pentoxide.
Above-mentioned bioactive minerals matter powder can be made by existing conventional techniques, such as the side that CN102826752A is recorded
Method.
Specifically, the collagen composite membrane of the present invention for guide tissue regeneration, it is by the bioactive minerals matter
Powder is evenly applied to be made on SIS thin-film materials.
In the present invention, it is defined as with SIS thin-film materials and the bioactive minerals matter powder coated thereon described
One layer of collagen composite membrane.
The collagen composite membrane can be multilayer, preferably 1-14 layers, more preferably 4-12 layers, more preferably 6-10
Layer.
Further, the weight of the bioactive minerals matter powder coated in every layer and the SIS thin-film materials
Ratio is 1:(1-99), preferably 1:(1-49)；Such as 1:99、1:50、1:30、1:5.
Further, crossed orientation arranges between every layer of SIS thin-film materials, and the SIS thin-film materials of even a certain layer are
Axially, then one layer adjacent of SIS thin-film materials are ring.
Further, the superiors of the collagen composite membrane are that multilayer (being preferably 1-4 layers) SIS thin-film materials (do not apply
Cover the bioactive minerals matter powder)；Further, crossed orientation arranges between these multilayers SIS thin-film materials, even
A certain layer SIS thin-film materials is axially, then one layer of adjacent SIS thin-film material are ring.
Because the axial direction of submucous layer of small intestine is different with the mechanical property of ring, intersects tiling and be beneficial to compound film dynamic performance
Be uniformly distributed.
The present invention also provides a kind of preparation method of such as above-mentioned collagen composite membrane for guide tissue regeneration, this method
Comprise the following steps：
(1) SIS thin-film materials (generally rectangle, approximate rectangle etc.) are taken to be laid on smooth flat board；
Usually, the flat board is made up of alloy materials such as high polymer material or stainless steels；
(2) above-mentioned bioactive minerals matter powder is evenly applied to the SIS thin-film material surfaces；Or by above-mentioned biology
Active mineral matter powder is uniformly mixed in gel systems in advance, and the mass fraction that bioactive minerals matter powder is made is
0.1%-20% hydrogel (being preferably the hydrogel that mass fraction is 5%-10%), then will contain bioactive minerals matter
The hydrogel of powder is evenly applied to the SIS thin-film material surfaces；
Wherein, the gel systems can be dissolved in the water by degradable water soluble macromolecular material and are prepared, institute
It can be hyaluronic acid, water soluble chitosan, collagen, gelatin etc. to state degradable water soluble macromolecular material；
Further, the weight of the bioactive minerals matter powder coated in every layer and the SIS thin-film materials
Ratio is 1:(1-99), such as 1:99、1:50、1:10、1:5；
(3) it is another to take SIS thin-film materials to be laid in the bioactive minerals matter powder layer of step (2) preparation；By step
Suddenly (2) identical method coats the bioactive minerals matter powder；
Further, crossed orientation arranges between every layer of SIS thin-film materials, and the SIS thin-film materials of even a certain layer are
Axially, then one layer adjacent of SIS thin-film materials are ring；
(4) collagen composite membrane for guide tissue regeneration of the different numbers of plies can be prepared (such as by repeating above step
Shown in Fig. 1).
The number of plies can be multilayer, preferably 1-14 layers, more preferably 4-12 layers, more preferably 6-10 layers.
Further, the above method also include will be prepared described in be used for guide tissue regeneration collagen composite membrane carry out
Dry step.
The drying means can be various conventional drying methods, such as hot dry, vacuum drying, air-dried, lyophilized or do naturally
It is dry.
Further, present invention research is found, by controlling drying condition to control each of the collagen composite membrane
Layer or several layers of compound fine and close porousnesses.Each layer of different fine and close porousnesses, which is combined with each other, can obtain all size
Collagen composite membrane, such as the two-sided collagen composite membrane that the fine and close another side of one side is loose.
Specifically, a kind of drying means is dry (for example with drying box) for heat, and temperature can be set to 25-40 DEG C, drying time
8-36h；Another drying means is alternatively lyophilized.
Preferably to improve the performance of the collagen composite membrane, SIS thin-film materials can also be done to further feature and changed
Property.
Specifically, SIS thin-film materials are soaked in modifying agent in advance before laying, SIS surfaces is carried active amino
Or polycation, obtain functional film component；The modifying agent is selected from polylysine, polylysine-aspartic acid copolymer
Or the one or more in chitosan solution.Pass through polylysine, polylysine-aspartic acid copolymer or chitosan surface band
Some active amino or positive charge assign material bilateral different features, obtain that toxicity is low, and cell compatibility is good, has function
The tissue renovation material of property.
The total concentration scope of the modifying agent is 0.001%-40%, because the total concentration of modifying agent is relevant with molecular weight,
As molecular weight is low, then modifier concentration accordingly improves, and it is suitable that those skilled in the art can select according to the professional general knowledge grasped
Modifier molecules amount and concentration, preferably 0.001%-32%；Active ingredient can mix in any proportion in this concentration range；
The soak time is 10min-24h, preferred scope 1h-4h；Preferably, the modifying agent is polylysin solution, its concentration
For 0.5%, 2h is soaked；The polylysine or polylysine-aspartic acid copolymer molecular weight are 1000-100000, described
Chitosan molecule amount is 3000-200000.
It is above-mentioned to have the advantages that SIS thin-film material modifications：
1st, the native three dimensional structure of de- extracellular matrix is maintained, is advantageous to growth, propagation and the differentiation of cell.
2nd, the active amino that modifying agent polylysine, polylysine-aspartic acid copolymer or chitosan surface carry, by
Its SIS composite after handling can promote creeping and growing for cell, can be used as guide tissue regeneration material and guiding bone
Regrown material.
Present invention additionally comprises the collagen composite membrane for guide tissue regeneration prepared by the above method.
Present invention additionally comprises the above-mentioned collagen composite membrane for guide tissue regeneration to prepare with guide tissue regeneration
Application in terms of functional material.
The present invention is used for the collagen composite membrane of guide tissue regeneration or the functional material with guide tissue regeneration can
For fields such as oral cavity, orthopaedics.
Viscosity of the present invention can be detected by this area conventional method, such as according to《Chinese Pharmacopoeia》Crow in (2015 editions)
Family name's viscometer method detects.
Provided by the present invention for the collagen composite membrane of guide tissue regeneration therapeutic effect be specifically described it is as follows：
1st, composite membrane provided by the invention can be used for guide tissue regeneration.The present invention is SIS materials and bioactive minerals
The composite membrane of matter powder, the wherein one side of SIS materials can effectively stop soft tissue, and guiding soft tissue prevents along film surface growth
Soft tissue is too fast to grow into skeletonization region, and good barrier action is provided for osteanagenesis, maintains space needed for osteanagenesis；It is in vivo
Degradation speed is 2-6 months, and the support of enough time is provided for osteanagenesis；Meanwhile it is compounded with bioactive minerals matter powder
Simultaneously, because bioactive minerals matter powder has the activity of unique bone induction and regeneration, osteoblastic proliferation can be promoted, accelerated
Knitting.
2nd, the present composition can be used for guiding bone regeneration while oral cavity implanting tooth, be planted at once after extraction
Bone regeneration around implant guided bone regeneration, bone after bone regeneration around implant guided bone regeneration, extraction during plant during progress extension implant
Guided bone regeneration, local alveolar ridge augmentation, alveolar ridge amplification art, the radectomyl radiectomy in Lie Xing Cranial defects area, oral cavity tumour
The filling in the Cranial defect region after resection and undesirable root arrachement, periodontal bone defects etc..Also can combine with various bone fillers makes
With.
3rd, can prevent while composition guide tissue regeneration provided by the invention in art or postoperative infection.Quasi-nano is given birth to
Thing active mineral matter powder has broad-spectrum antibacterial, can suppress intraoral Actinobacillus, streptococcus, porphyromonas gingivalis,
The oral cavity pathogens such as Prevotella intermedia, treponema denticola, Post operation is neutralized due to thin so as to which said composition can prevent operation
Microbial infection.
The present invention has advantages below：
1st, SIS materials and bioactive minerals matter the powder combination that composition provided by the invention uses, said composition is not
Only bootable regeneration, while Bone Defect Repari is can induce, it is specific as follows：Bioactive minerals matter powder and SIS in said composition
In collagenous fibres combine, into after human body defect, along with the degraded of SIS materials, bioactive minerals matter powder delays
On The Drug Release, bioactive minerals matter powder is with after bioresorbable, ion exchange can occur immediately in its particle surface, so as in bone
The apatite gel layer containing bicarbonate is produced with bioactive minerals matter powder surface, Self substances can be considered by body
And received, and then the autocrine mediated response of osteocyte is stimulated, cytokine profiles are produced, finally cause the rapid propagation of bone.Separately
Outside, HCA generation can cause the precipitation of collagenous fibres, thus caused collagen can be precipitated constantly in SIS degradation process,
Multiple bone islands are gradually formed with autologous bone and collagenous fibres under the stimulation of cell factor, these bone islands are further connected, most
After complete Bone Defect Repari.Thus simultaneously non-individual works the material of the present composition, but is sent out in bioactive minerals matter powder
After raw ion exchange forms HCA, complement one another, so as to realize faster reparation, while there is osteoinductive.
2nd, SIS promotes endogenous tissue regenerative process in vivo, realizes the reproducibility reparation to defect, and SIS is mainly day
So, the collagen component of degradable absorption, there is low antigenicity, good histocompatbility and mechanical compliance；SIS pollution or
Infected zone early promotion new vessels is formed, and enhancing tissue is to the tolerance that pollutes and infect.；Bioactive minerals matter powder
With bacteriostasis, suppress common pathogen in oral cavity, surgical site infection risk can be greatly reduced in the two combination.
3rd, the present invention provides the preparation method for preparing said composition, SIS bioactive minerals matter powders for thin-film material
For solid powder, mode is not used in combination well clinically.Preparation method resulting composition provided by the invention is thin
Membranaceous, soft texture, bioactive minerals matter powder complements each other with SIS materials and combined closely, and facilitates Clinical practice.It is and compound
The SIS materials of bioactive minerals matter powders make guide tissue regeneration film be provided with preferably promote the effect of skeletonization with it is good
Anti-infectious function.
4th, compared to the guide tissue regeneration collagem membrane being directly prepared into by collagen, new guiding tissue provided by the invention is again
Filming has good mechanical strength, and the mechanical strength of cladding SIS films is prepared according to the preparation method of invention description, can meet to draw
Lead the Clinical practice requirement of regeneration art.
Brief description of the drawings
Fig. 1 is the structural representation for the collagen composite membrane that the present invention is used for guide tissue regeneration；
Wherein, 1 is SIS film material plies, and 2 are biological active mineral matter powder layer or contain bioactive minerals matter powder
Gel layer；
Fig. 2 is the experimental result picture of experimental example 1.
Fig. 3 is the scanning electron microscope (SEM) photograph for the collagen composite membrane that embodiment 1 is used for guide tissue regeneration；
It is surface partial enlarged drawing wherein to scheme A, and figure B is sectional drawing, and figure C is SIS collagenous fibres enlarged drawings.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.It is unreceipted specific in embodiment
Technology or condition person, carried out according to the technology or condition described by document in the art, or according to product description.It is used
Reagent or the unreceipted production firm person of instrument, it is the conventional products that can be commercially available by regular distributor.
It is prepared by the method that SIS thin-film materials as described below are recorded by CN101433735A embodiments 1.
Bioactive minerals matter powder as described below, the composition containing following percentage by weight：SiO245%, CaO
24.5%, Na2O 24.5%, P2O56%；Further, its particle size range is less than 90 μm, wherein, containing aperture in 100-
Quasi-nano particle 2-5% in the range of 900nm；It is prepared by the method that can be recorded by CN102826752A.
Embodiment 1
A kind of collagen composite membrane for guide tissue regeneration, its preparation method comprise the following steps：
(1) SIS thin-film materials are taken to be laid on smooth flat board；The flat board is by high polymer material or stainless steel etc.
Alloy material is made；
(2) above-mentioned bioactive minerals matter powder is uniformly mixed in gel systems in advance, bioactive minerals is made
The mass fraction of matter powder is 10% hydrogel, then uniformly applies the hydrogel containing bioactive minerals matter powder
It is overlying on the SIS thin-film material surfaces；
Wherein, the gel systems can be dissolved in the water by degradable water soluble macromolecular material and are prepared, institute
It can be hyaluronic acid to state degradable water soluble macromolecular material；The coated bioactive minerals matter powder in every layer
Part by weight with the SIS thin-film materials is 1:10；
(3) it is another to take SIS thin-film materials to be laid in the bioactive minerals matter powder layer of step (2) preparation；By step
Suddenly (2) identical method coats the bioactive minerals matter powder；
(4) above step is repeated, crossed orientation arranges between making every layer of SIS thin-film materials, and the SIS of even a certain layer is thin
Membrane material is axially, then one layer adjacent of SIS thin-film materials is ring；Prepare the number of plies be 6 layers it is described be used for guide
The collagen composite membrane (as shown in Figure 1) of regeneration.Required product can be obtained by being dried again.
Embodiment 2
A kind of collagen composite membrane for guide tissue regeneration, its preparation method differ only in embodiment 1：Step
2) above-mentioned bioactive minerals matter powder is uniformly mixed in gel systems in advance, the matter of bioactive minerals matter powder is made
The hydrogel that fraction is 20% is measured, is then evenly applied to the hydrogel containing bioactive minerals matter powder described
SIS thin-film material surfaces；The weight ratio of the coated bioactive minerals matter powder and the SIS thin-film materials in every layer
Example is 1:50.
Embodiment 3
A kind of collagen composite membrane for guide tissue regeneration, its preparation method differ only in embodiment 1：Step
2) above-mentioned bioactive minerals matter powder is uniformly mixed in gel systems in advance, the matter of bioactive minerals matter powder is made
The hydrogel that fraction is 30% is measured, is then evenly applied to the hydrogel containing bioactive minerals matter powder described
SIS thin-film material surfaces；The weight ratio of the coated bioactive minerals matter powder and the SIS thin-film materials in every layer
Example is 1:30.
Embodiment 4
A kind of collagen composite membrane for guide tissue regeneration, its preparation method differ only in embodiment 1：Step
2) above-mentioned bioactive minerals matter powder is uniformly mixed in gel systems in advance, the matter of bioactive minerals matter powder is made
The hydrogel that fraction is 50% is measured, is then evenly applied to the hydrogel containing bioactive minerals matter powder described
SIS thin-film material surfaces；The weight ratio of the coated bioactive minerals matter powder and the SIS thin-film materials in every layer
Example is 1:5.
Comparative example 1
A kind of composite membrane, with differing only in for embodiment 1：Bioactive minerals matter powder and the SIS thin-film materials
Simple physical is mixed, and powder is applied into SIS material surfaces.
Comparative example 2
A kind of composite membrane, with differing only in for embodiment 1：The coated bioactive minerals matter powder in every layer
Part by weight with the SIS thin-film materials is 1:0.5.
Comparative example 3
A kind of composite membrane, with differing only in for embodiment 1：The coated bioactive minerals matter powder in every layer
Part by weight with the SIS thin-film materials is 1:100.
Experimental example 1：Guide tissue regeneration film animal experiment
Experiment material：Collagen composite membrane for guide tissue regeneration prepared by embodiment 1, comparative example 1 are prepared compound
Film；
Experimental method：Using the rabbit of mandibular defect as experimental subjects, above-mentioned experiment material is guided into regeneration reality
Test.
Experimental result：As shown in Fig. 2 the collagen composite membrane of embodiment 1 be used for defect after eight weeks defect be cured substantially
Close, and the composite membrane of comparative example 1 still has 4mm × 2mm irregular defect.Show, guide tissue regeneration significant effect of the present invention.
The guide tissue regeneration film mechanical strength of experimental example 2
The regeneration membrane respectively prepared by embodiment 1-4 and comparative example 1-3 carries out mechanical strength test.
Each regeneration membrane is cut into 1.5cm × 5cm strips, is sandwiched in mechanical test instrument test section, carries out tensile strength survey
Examination, draw speed 1mm/min.Method of testing is detection method commonly used in the art.
Test result such as following table：Unit N/cm
As a result show, guide tissue regeneration film mechanical strength prepared by the present invention is much larger than like product, more preferable to meet to face
Bed demand.
Experimental example 3
The scanning electron microscope (SEM) photograph of collagen composite membrane for guide tissue regeneration prepared by embodiment 1 is shown in Fig. 3, can from sectional drawing
Being clearly visible guide tissue regeneration film has double-layer structure, and its loose one side is amplified, a fixed number is combined on its collagenous fibres
The bioactive minerals matter powder of amount.
Claims (10)
1. a kind of collagen composite membrane for guide tissue regeneration, it is characterised in that be that bioactive minerals matter powder is uniform
Coated on manufactured multilayer film on SIS thin-film materials；
The percentage by weight that the bioactive minerals matter powder accounts for the collagen composite membrane is 0.01%~50%；
The bioactive minerals matter powder, the composition containing following percentage by weight：SiO240-65%, CaO 15-30%,
Na2O 15-30%, P2O52-8%, its particle size range are less than 90 μm, wherein, the standard containing aperture in the range of 100-900nm
Nano-scale particle 0.1-20.0wt%.
2. it is used for the collagen composite membrane of guide tissue regeneration according to claim 1, it is characterised in that the bioactivity ore deposit
The percentage by weight that material powder accounts for the collagen composite membrane is 1%~30%.
3. the collagen composite membrane according to claim 1 or claim 2 for guide tissue regeneration, it is characterised in that the collagen is answered
Conjunction film is 1-14 layers, preferably 4-12 layers, more preferably 6-10 layers.
4. it is used for the collagen composite membrane of guide tissue regeneration according to claim any one of 1-3, it is characterised in that in every layer
The part by weight of the coated bioactive minerals matter powder and the SIS thin-film materials is 1:(1-99), preferably 1:
(1-49)。
5. it is used for the collagen composite membrane of guide tissue regeneration according to claim any one of 1-4, it is characterised in that every layer
Crossed orientation arranges between SIS thin-film materials；
Preferably, the superiors of the collagen composite membrane are multilayer SIS thin-film materials；It is highly preferred that these multilayers SIS film materials
Crossed orientation arranges between material.
6. it is used for the collagen composite membrane of guide tissue regeneration according to claim any one of 1-5, it is characterised in that the life
Thing active mineral matter powder, the composition containing following percentage by weight：SiO245-61%, CaO 17-27%, Na2O 19-
25%, P2O52.6-6%；Further, its particle size range is less than 90 μm, wherein, containing aperture in the range of 100-900nm
Quasi-nano particle 2-10%；
Preferably, the bioactive minerals matter powder, the composition containing following percentage by weight：SiO245%, CaO
24.5%, Na2O 24.5%, P2O56%；Further, its particle size range is less than 90 μm, wherein, containing aperture in 100-
Quasi-nano particle 2-5% in the range of 900nm.
7. it is used for the collagen composite membrane of guide tissue regeneration according to claim any one of 1-6, it is characterised in that described
Modified dose of immersion is modified SIS thin-film materials in advance, SIS surfaces is carried active amino or polycation；The modifying agent choosing
One or more from polylysine, polylysine-aspartic acid copolymer or chitosan solution.
8. it is used for the preparation method of the collagen composite membrane of guide tissue regeneration described in claim any one of 1-7, it is characterised in that
Comprise the following steps：
(1) SIS thin-film materials are taken to be laid on smooth flat board；
(2) bioactive minerals matter powder is evenly applied to the SIS thin-film material surfaces；Or by above-mentioned bioactive minerals
Matter powder is uniformly mixed in gel systems in advance, and the mass fraction that bioactive minerals matter powder is made is 0.1%-50%'s
Hydrogel, the hydrogel containing bioactive minerals matter powder is then evenly applied to the SIS thin-film material surfaces；
Wherein, the gel systems can be dissolved in the water by degradable water-soluble high-molecular material and are prepared, described to drop
It can be hyaluronic acid, water soluble chitosan, collagen, gelatin to solve water-soluble high-molecular material；
(3) it is another to take SIS thin-film materials to be laid in the bioactive minerals matter powder layer of step (2) preparation；By step (2)
Identical method coats the bioactive minerals matter powder；
(4) collagen composite membrane for guide tissue regeneration of the different numbers of plies can be prepared by repeating above step.
9. preparation method according to claim 8, it is characterised in that also include will be prepared described in be used for guide tissue
The step of collagen composite membrane of regeneration is dried；The drying is dry for heat, and temperature is 25-40 DEG C, drying time 8-36h；Or
Drying means described in person is lyophilized.
10. the collagen composite membrane for guide tissue regeneration or the side of claim 8 or 9 described in claim any one of 1-7
Collagen composite membrane for guide tissue regeneration prepared by method answering in terms of the functional material with guide tissue regeneration is prepared
With.
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| CN116617471B (en) * | 2023-07-24 | 2023-10-13 | 成都贝施美医疗科技股份有限公司 | Oral collagen membrane with double-layer structure and preparation method thereof |
| CN118892582A (en) * | 2024-08-19 | 2024-11-05 | 卓阮医疗科技（苏州）有限公司 | A preparation method for keratinized gingival repair stent |
| CN118892582B (en) * | 2024-08-19 | 2025-03-11 | 卓阮医疗科技（苏州）有限公司 | A preparation method for keratinized gingival repair stent |
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| CN107875454B (en) | 2020-12-11 |
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