CN107823114A - A kind of anti-acne skin care item containing sealwort stem cell extract - Google Patents
A kind of anti-acne skin care item containing sealwort stem cell extract Download PDFInfo
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- CN107823114A CN107823114A CN201711312664.1A CN201711312664A CN107823114A CN 107823114 A CN107823114 A CN 107823114A CN 201711312664 A CN201711312664 A CN 201711312664A CN 107823114 A CN107823114 A CN 107823114A
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
A kind of skin care item the invention discloses acne-removing composition and containing composition, the composition contain the composition of following parts by weight：1.2~10.7 parts of 0.03~2.9 part of sealwort stem cell extract, 0.6~7.1 part of Cortex Meliae extract, 0.75~8.2 part of white Herba Lysimachiae foenumgraeci extract, 0.9~9.1 part of winter bamboo shoot extract and dragon tree extract.The skin care item of the present invention contain sealwort stem cell extract, Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract and dragon tree extract, can effectively dispel face acnes；In addition, the skin care item of the present invention are free of preservative, it is nontoxic to skin safe, while there is oxidation resistant effect.
Description
Technical field
The present invention relates to technical field of skin care, especially a kind of anti-acne skin care item containing sealwort stem cell extract.
Background technology
Acne, whelk, acne, dark sore are commonly called as, traditional Chinese medical science ancient times claim face sore, acne, are dermatology common diseases, frequently-occurring disease.
During puberty, internal hormone can stimulate hair growth to promote smegma more many greases of sauce, hair and sebaceous glands therefore heap
The many materials of product, make grease and the attached work of bacterium, trigger the reaction of red swelling of the skin, because this symptom is common in youth of both sexes, institute
Using often it be referred to as " whelk ".
Counted according to scholars, have 95% in Adolescent male, women has 85% to suffer from different degrees of acne.So everybody
It is very proper to be called " whelk ".Acne (whelk) is a kind of chronic dermatosis for betiding pilosebaceous follicle, multiple
In Head And Face, the abundant position of the sebaceous glands such as neck, shirtfront back.The main clinical manifestation of acne (whelk) is blackhead powder
Thorn, Whiteheads, inflamed papules, warts, tubercle, tumour, easily formation pigmentation, pore are thick or even scar sample damages.Shadow
Beauty is rung, severe patient can cause to disfeature, and great psychological pressure and mental suffering are caused to young man.
Except whelk, many adults also president's acnes, in medical science=it is referred to as adult's acne：Also referred to as " rear puberty small pox ",
More with environment change, Pressure stimulation, bad habits and customs (such as the pungent food such as hobby excitant food, Sichuan cuisine and Hunan cuisine
Thing), the day and night reverse daily schedule it is relevant, and be mainly in chin, the U-shaped area near labial angle.Be grown up acne often with pressure, sleep not
Metabolism is bad in terms of the body and mind such as foot, fatigue, lutern hypersecretion, constipation substantial connection.Acne of being grown up occurs with women
Likelihood ratio male it is high, the urban woman of 25-35 year Combination skin is easiest to grow adult's acne.For acne of being grown up
Crack cause：On the one hand it is as caused by cosmetics improper use, the pressure that secondly human body is born turns into greatly long small pox indirectly
Arch-criminal.
On October 25th, 2010, Nature Biotechnology have delivered the research institute of the Unhwa Corp companies of South Korea
It is successfully separated first and has cultivated the paper of plant stem cell, and certification has been obtained to the identity of plant stem cell, plant does
The research of cell enters the stage of high speed development.
In simple terms, there are two " group " cells for being located at plant tip in plant, they have powerful self-renewing
Ability, it can not only be divided into autogenous cell, moreover it is possible to other any plant cells are divided into, are qualified " general-purpose cells ",
Moreover, they, which also have, continues splitting ability so that many large-scale plant existences more than thousand.
2012, " plant stem cell " technology was studied in the effect of beauty and health care field and has been achieved for certain progress,
Metabolism of the plant stem cell to raising skin is proved, improves microcirculation, tightening up and recovering skin elasticity has major application
Value.
The content of the invention
Based on this, one kind is provided and can effectively be dispelled it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
The skin care item of whelk and adult's acne, the skin care item are safe and non-toxic to skin, will not cause allergic.
To achieve the above object, this invention takes following technical scheme：
As the first aspect of the invention, the invention provides a kind of acne-removing composition, the composition contains following
The composition of parts by weight：0.03~2.9 part of sealwort stem cell extract, 0.6~7.1 part of Cortex Meliae extract, the extraction of white lavender
1.2~10.7 parts of 0.75~8.2 part of thing, 0.9~9.1 part of winter bamboo shoot extract and dragon tree extract.
Wherein, sealwort has the function that antifatigue, regulation blood glucose；Sealwort stem cell can pass through epigenetic factor pair people
Class Skin Cell plays interference effect, can effectively encourage the power of regeneration of skin progenitor cell, hyperplasia fibrillar structure albumen, accelerate flesh
Self more raw and reparation of skin；Azedarach can be used for treating roundworm disease, enterobiasis, abdominal pain due to enterositosis and control mange itch outside；Smoke clothing
Main component linalool in careless essential oil can suppression microbicide activity, available for preserving fruit and vegetable utilizing, to people and the nontoxic nothing of mammal
Residual, to grain and environmental nonpollution, will can also turn into a kind of food preservative of novel green；Lavender is because of its gas
Taste fragrance, obvious repellent action is also played to mosquito；Winter bamboo shoot contain abundant protein and several amino acids, vitamin,
And the trace element such as calcium, phosphorus, iron and abundant cellulose, intestines peristalsis can be promoted, both contributed to digest, having can prevent just
The secret and generation of colon cancer, the polysaccharide material contained by it, also with certain antitumaous effect；One kind can be flowed out after dragon tree injury
The liquid of color, this liquid are a kind of resins, kermesinus, are a kind of rare Chinese medicine, Chinese medicine entitled " dragon's blood " or " kylin
Exhaust ", there are medicinal blood circulation-promoting functions, arthralgia and myalgia can be treated；It is, in general, that monocotyledon length to a certain extent afterwards cannot
Continue diameter increment, though dragon tree belongs to monocotyledon, the parenchyma cell in its stem can constantly divide, and make stem year by year
Overstriking and lignifying, and form arbor.
Preferably, the mass ratio of the sealwort stem cell extract, Cortex Meliae extract and white Herba Lysimachiae foenumgraeci extract is
0.15~1.6：2.4：1.1~5.7.It is highly preferred that sealwort stem cell extract, Cortex Meliae extract and the white lavender carry
The mass ratio for taking thing is 0.39：2.4：4.5.Present inventor has found through test of many times, when sealwort stem cell in skin care item
The mass ratio of extract, Cortex Meliae extract and white Herba Lysimachiae foenumgraeci extract is 0.15~1.6：2.4：When 1.1~5.7, skin care item
Anti-acne effect it is preferable；And when the matter of sealwort stem cell extract, Cortex Meliae extract and white Herba Lysimachiae foenumgraeci extract in skin care item
Amount is than being 0.39：2.4：When 4.5, the anti-acne effect of skin care item is more preferably.
Preferably, the mass ratio of the sealwort stem cell extract and dragon tree extract is 0.5~1.7：6.1.It is more excellent
Selection of land, the mass ratio of the sealwort stem cell extract and dragon tree extract is 0.67：6.1.Present inventor's warp is more
Secondary experiment is found, when the mass ratio of sealwort stem cell extract and dragon tree extract is 0.5~1.7 in skin care item：When 6.1,
The antioxidant effect of skin care item is preferable；And work as the mass ratio of sealwort stem cell extract and dragon tree extract in skin care item and be
0.67：When 6.1, the antioxidant effect of skin care item is more preferably.
Preferably, the composition contains the composition of following parts by weight：0.39 part of sealwort stem cell extract, azedarach carry
Take 3.55 parts of 2.4 parts of thing, 4.5 parts of white Herba Lysimachiae foenumgraeci extract, 8.2 parts of winter bamboo shoot extract and dragon tree extract.The hair of the application
A person of good sense has found through test of many times, when containing 0.39 part of sealwort stem cell extract, 2.4 parts of Cortex Meliae extract, white in skin care item
When 3.55 parts of 4.5 parts of Herba Lysimachiae foenumgraeci extract, 8.2 parts of winter bamboo shoot extract and dragon tree extract, the anti-acne effect of skin care item and anti-
Oxidation effectiveness is all good.
As the second aspect of the invention, the invention provides a kind of anti-acne skin care item, the skin care item include above-mentioned
Composition.
Preferably, sealwort stem cell extract, Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot in the skin care item
The quality percentage total amount of extract and dragon tree extract is 8.1~10.3%.Present inventor has found through test of many times,
When sealwort stem cell extract, Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract and dragon tree extraction in skin care item
When the quality percentage total amount of thing is 8.1~10.3%, the anti-acne effect of skin care item is more preferably.
Preferably, the sealwort stem cell extract is prepared by the following：
S1, sealwort new life root or branch sterilization, after removing xylem and marrow, it is inoculated in inducing culture, Fiber differentiation
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell extract through the micro-nano method broken wall of low temperature.
Wherein, the micro-nano method broken wall of low temperature refers to make its freezing in a low temperature of sealwort stem cell is placed on into -170 DEG C, then
Melt at normal temperatures, so repeatedly so that the cell membrane of sealwort stem cell crushes；This method can by the vital activity of stem cell with
Power of regeneration is assigned in skin care item, is absorbed via skin, can be remarkably promoted nutrition and be penetrated into and absorb, can effectively lift skin actively
Property, improve skin quality from cell aspect, can accelerate to dispel the efficiency of acne, avoid the generation of phenomena of cutaneous irritation.
Preferably, the dragon tree extract is prepared by the following：Dragon's blood leaf is adopted after high speed bead mill method broken wall
Dragon tree extract is obtained with water extraction；
The winter bamboo shoot extract is prepared by the following：Winter bamboo shoot pulp is taken to be obtained after ultrasonication using alcohol extracting method
Winter bamboo shoot extract；
The white Herba Lysimachiae foenumgraeci extract is prepared by the following：White lavender is taken to be adopted after the micro-nano method broken wall of low temperature
White Herba Lysimachiae foenumgraeci extract is obtained with water extraction；
The Cortex Meliae extract is prepared by the following：Azedarach is taken after the micro-nano method broken wall of low temperature, using alcohol
Formulation obtains Cortex Meliae extract.Wherein, extracted in the alcohol extracting method using methanol or ethanol
Relative to prior art, beneficial effects of the present invention are：
The skin care item of the present invention contain sealwort stem cell extract, Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot and carried
Thing and dragon tree extract are taken, can effectively dispel face acnes；In addition, the skin care item of the present invention are free of preservative, skin is pacified
Atoxic, while there is oxidation resistant effect.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of acne-removing composition in the present invention, the composition contain the composition of following parts by weight：Sealwort
0.03 part of stem cell extract, 7.1 parts of Cortex Meliae extract, 0.75 part of white Herba Lysimachiae foenumgraeci extract, 9.1 parts of winter bamboo shoot extract and dragon
1.2 parts of blood tree extract.
A kind of embodiment of anti-acne skin care item in the present invention, the skin care item include above-mentioned composition, 39 parts of saualane, sweet
101 parts of 22 parts of oil, 43 parts of Arabic gum and water, wherein, sealwort stem cell extract, Cortex Meliae extract, the extraction of white lavender
The percentage that the gross mass of thing, winter bamboo shoot extract and dragon tree extract accounts for skin care quality is 11.5%.
The specific preparation method of above-mentioned anti-acne skin care item comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain sealwort stem cell extract through the micro-nano method broken wall of low temperature.
(2) dragon tree extract is prepared by the following：Dragon's blood leaf is after high speed bead mill method broken wall, using water extraction
Obtain dragon tree extract；
Winter bamboo shoot extract is prepared by the following：Winter bamboo shoot pulp is taken to be obtained after ultrasonication using methanol extraction
Winter bamboo shoot extract；
White Herba Lysimachiae foenumgraeci extract is prepared by the following：White lavender is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains white Herba Lysimachiae foenumgraeci extract；
Cortex Meliae extract is prepared by the following：Azedarach is taken to be carried after the micro-nano method broken wall of low temperature using ethanol
Follow the example of to obtain Cortex Meliae extract.
(3) by Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract and dragon tree extract and saualane 39
Part, 22 parts of glycerine, 43 parts of Arabic gum and 101 parts of mixing of water, after 37.4 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-acne face cream.
Embodiment 2
A kind of embodiment of acne-removing composition in the present invention, the composition contain the composition of following parts by weight：Sealwort
0.17 part of stem cell extract, 5.3 parts of Cortex Meliae extract, 2.1 parts of white Herba Lysimachiae foenumgraeci extract, 6.8 parts of winter bamboo shoot extract and dragon
5.9 parts of blood tree extract.
A kind of embodiment of anti-acne skin care item in the present invention, the skin care item include above-mentioned composition, 48 parts of saualane, sweet
121 parts of 31 parts of oil, 52 parts of Arabic gum and water, wherein, sealwort stem cell extract, Cortex Meliae extract, the extraction of white lavender
The percentage that the gross mass of thing, winter bamboo shoot extract and dragon tree extract accounts for skin care quality is 10.3%.
The specific preparation method of above-mentioned anti-acne skin care item comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain sealwort stem cell extract through the micro-nano method broken wall of low temperature.
(2) dragon tree extract is prepared by the following：Dragon's blood leaf is after high speed bead mill method broken wall, using water extraction
Obtain dragon tree extract；
Winter bamboo shoot extract is prepared by the following：Winter bamboo shoot pulp is taken to be obtained after ultrasonication using ethanol extraction method
Winter bamboo shoot extract；
White Herba Lysimachiae foenumgraeci extract is prepared by the following：White lavender is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains white Herba Lysimachiae foenumgraeci extract；
Cortex Meliae extract is prepared by the following：Azedarach is taken to be carried after the micro-nano method broken wall of low temperature using methanol
Follow the example of to obtain Cortex Meliae extract.
(3) by Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract and dragon tree extract and saualane 48
Part, 31 parts of glycerine, 52 parts of Arabic gum and 121 parts of mixing of water, after 36.7 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-acne face cream.
Embodiment 3
A kind of embodiment of acne-removing composition in the present invention, the composition contain the composition of following parts by weight：Sealwort
0.39 part of stem cell extract, 2.4 parts of Cortex Meliae extract, 4.5 parts of white Herba Lysimachiae foenumgraeci extract, 8.2 parts of winter bamboo shoot extract and dragon
3.55 parts of blood tree extract.
A kind of embodiment of anti-acne skin care item in the present invention, the skin care item include above-mentioned composition, 51 parts of saualane, sweet
116 parts of 20 parts of oil, 54 parts of Arabic gum and water, wherein, sealwort stem cell extract, Cortex Meliae extract, the extraction of white lavender
The percentage that the gross mass of thing, winter bamboo shoot extract and dragon tree extract accounts for skin care quality is 9.5%.
The specific preparation method of above-mentioned anti-acne skin care item comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain sealwort stem cell extract through the micro-nano method broken wall of low temperature.
(2) dragon tree extract is prepared by the following：Dragon's blood leaf is after high speed bead mill method broken wall, using water extraction
Obtain dragon tree extract；
Winter bamboo shoot extract is prepared by the following：Winter bamboo shoot pulp is taken to be obtained after ultrasonication using ethanol extraction method
Winter bamboo shoot extract；
White Herba Lysimachiae foenumgraeci extract is prepared by the following：White lavender is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains white Herba Lysimachiae foenumgraeci extract；
Cortex Meliae extract is prepared by the following：Azedarach is taken to be carried after the micro-nano method broken wall of low temperature using ethanol
Follow the example of to obtain Cortex Meliae extract.
(3) by Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract and dragon tree extract and saualane 51
Part, 20 parts of glycerine, 54 parts of Arabic gum and 116 parts of mixing of water, after 37 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-acne face cream.
Embodiment 4
A kind of embodiment of acne-removing composition in the present invention, the composition contain the composition of following parts by weight：Sealwort
1.5 parts of stem cell extract, 1.5 parts of Cortex Meliae extract, 6.3 parts of white Herba Lysimachiae foenumgraeci extract, 3.4 parts of winter bamboo shoot extract and dragon's blood
Set 7.6 parts of extract.
A kind of embodiment of anti-acne skin care item in the present invention, the skin care item include above-mentioned composition, 52 parts of saualane, sweet
123 parts of 29 parts of oil, 61 parts of Arabic gum and water, wherein, sealwort stem cell extract, Cortex Meliae extract, the extraction of white lavender
The percentage that the gross mass of thing, winter bamboo shoot extract and dragon tree extract accounts for skin care quality is 8.9%.
The specific preparation method of above-mentioned anti-acne skin care item comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain sealwort stem cell extract through the micro-nano method broken wall of low temperature.
(2) dragon tree extract is prepared by the following：Dragon's blood leaf is after high speed bead mill method broken wall, using water extraction
Obtain dragon tree extract；
Winter bamboo shoot extract is prepared by the following：Winter bamboo shoot pulp is taken to be obtained after ultrasonication using methanol extraction
Winter bamboo shoot extract；
White Herba Lysimachiae foenumgraeci extract is prepared by the following：White lavender is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains white Herba Lysimachiae foenumgraeci extract；
Cortex Meliae extract is prepared by the following：Azedarach is taken to be carried after the micro-nano method broken wall of low temperature using methanol
Follow the example of to obtain Cortex Meliae extract.
(3) by Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract and dragon tree extract and saualane 52
Part, 29 parts of glycerine, 61 parts of Arabic gum and 123 parts of mixing of water, after 37.5 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-acne face cream.
Embodiment 5
A kind of embodiment of acne-removing composition in the present invention, the composition contain the composition of following parts by weight：Sealwort
2.9 parts of stem cell extract, 0.6 part of Cortex Meliae extract, 8.2 parts of white Herba Lysimachiae foenumgraeci extract, 0.9 part of winter bamboo shoot extract and dragon's blood
Set 10.7 parts of extract.
A kind of embodiment of anti-acne skin care item in the present invention, the skin care item include above-mentioned composition, 63 parts of saualane, sweet
127 parts of 31 parts of oil, 74 parts of Arabic gum and water, wherein, sealwort stem cell extract, Cortex Meliae extract, the extraction of white lavender
The percentage that the gross mass of thing, winter bamboo shoot extract and dragon tree extract accounts for skin care quality is 8.1%.
The specific preparation method of above-mentioned anti-acne skin care item comprises the following steps：
(1) sealwort stem cell extract is prepared by the following：
S1, the sterilization of sealwort new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain sealwort stem cell extract through the micro-nano method broken wall of low temperature.
(2) dragon tree extract is prepared by the following：Dragon's blood leaf is after high speed bead mill method broken wall, using water extraction
Obtain dragon tree extract；
Winter bamboo shoot extract is prepared by the following：Winter bamboo shoot pulp is taken to be obtained after ultrasonication using ethanol extraction method
Winter bamboo shoot extract；
White Herba Lysimachiae foenumgraeci extract is prepared by the following：White lavender is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains white Herba Lysimachiae foenumgraeci extract；
Cortex Meliae extract is prepared by the following：Azedarach is taken to be carried after the micro-nano method broken wall of low temperature using ethanol
Follow the example of to obtain Cortex Meliae extract.
(3) by Cortex Meliae extract, white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract and dragon tree extract and saualane 63
Part, 31 parts of glycerine, 74 parts of Arabic gum and 127 parts of mixing of water, after 38 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition sealwort stem cell extract, which is uniformly dispersed, produces a kind of anti-acne face cream.
The anti-acne effect test of the anti-acne face cream of the present invention of embodiment 6
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, the shield of control group 1~4
Differ only in sealwort stem cell extract, Cortex Meliae extract and the white lavender of skin frost and the face cream of embodiment 3 are extracted
The mass ratio of thing is different, and its preparation method is same as Example 3；The sealwort stem cell extract of the face cream of control group 1~4,
The mass ratio of Cortex Meliae extract and white Herba Lysimachiae foenumgraeci extract is followed successively by：0.15:2.4:1.1；0.78：2.4：2.3；1.3：2.4：
3.5；1.6：2.4：5.7；
Method of testing：If choosing, 15~21 years old more youth of both sexes of the long acne of face are some and the men and women that grows up for 25~31 years old
It is dry, it is divided into nine groups altogether, every group of 12 people, statistics uses the quantity of face cream front face acne, and nine groups of personnel use embodiment 1 respectively
~5 face cream and the face cream of control group 1~4, once in the morning and once at night, after 50 days, the quantity of facial acne is counted, calculating makes
With the par of front and rear facial acne reduction.
Test result：It is as shown in table 1 below；
The anti-acne effect of face cream in the present invention of table 1
| Face cream | Acne averagely reduces quantity | Face cream | Acne averagely reduces quantity |
| Embodiment 1 | 1.19 | Control group 1 | 4.01 |
| Embodiment 2 | 2.25 | Control group 2 | 5.27 |
| Embodiment 3 | 6.9 | Control group 3 | 4.54 |
| Embodiment 4 | 3.16 | Control group 4 | 4.13 |
| Embodiment 5 | 2.87 |
Interpretation of result：As shown in Table 1, the face cream in the present invention can dispel face acnes to a certain extent, wherein
The anti-acne effect of the face cream of embodiment 3 and the face cream of control group 1~4 is preferable, corresponding sealwort stem cell extract, China tree
The mass ratio of bark extract and white Herba Lysimachiae foenumgraeci extract all falls within 0.15~1.6：2.4：In the range of 1.1~5.7；Also, implement
The anti-acne ability of the face cream of example 3 is best, wherein sealwort stem cell extract, Cortex Meliae extract and white Herba Lysimachiae foenumgraeci extract
Mass ratio is 0.39：2.4：4.5.
The antioxidant effect test of the anti-acne face cream of the present invention of embodiment 7
Test object：The face cream of embodiment 1~5 and as a control group 1~4 face cream, wherein, control group 1~4
Face cream differs only in the mass ratio of sealwort stem cell extract and dragon tree extract compared with the face cream of embodiment 3
Difference, the other compositions and preparation method of face cream are identical with the face cream of embodiment 3；Sealwort in the face cream of control group 1~4
The mass ratio of stem cell extract and dragon tree extract is followed successively by 0.5：6.1；0.93：6.1；1.35：6.1；1.7：6.1；
Method of testing：ORAC methods；Specifically, the face cream of Example 1~5 and 1~4 skin care as a control group respectively
Frost, it is configured to the aqueous solution, it is desirable to which extract total concentration is identical in the face cream aqueous solution, such as 1.6g/L, is used respectively using preceding
72mmol/L phosphate buffers (pH=7.2) dilute 1000 times and are used as test sample solution.
Test result：It is as shown in table 2 below；
The oxidation resistance test result of the face cream of the present invention of table 2
| Face cream | ORAC values | Face cream | ORAC values |
| Embodiment 1 | 544 | Control group 1 | 1129 |
| Embodiment 2 | 736 | Control group 2 | 1063 |
| Embodiment 3 | 1485 | Control group 3 | 983 |
| Embodiment 4 | 1018 | Control group 4 | 915 |
| Embodiment 5 | 921 |
Interpretation of result：It was found from upper table 2, the ORAC values of the face cream of embodiment 3,4,5 and control group 1~4 are higher, table
Show that the oxidation resistance of the face cream of embodiment 3~5 and control group 1~4 is preferable；Wherein, with the anti-of the face cream of embodiment 3
Oxidability is optimal.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of acne-removing composition, it is characterised in that the composition contains the composition of following parts by weight：Sealwort stem cell is extracted
0.03~2.9 part of thing, 0.6~7.1 part of Cortex Meliae extract, 0.75~8.2 part of white Herba Lysimachiae foenumgraeci extract, winter bamboo shoot extract 0.9
~9.1 parts and 1.2~10.7 parts of dragon tree extract.
2. composition according to claim 1, it is characterised in that the sealwort stem cell extract, Cortex Meliae extract
Mass ratio with white Herba Lysimachiae foenumgraeci extract is 0.15~1.6：2.4：1.1~5.7.
3. composition according to claim 2, it is characterised in that the sealwort stem cell extract, Cortex Meliae extract
Mass ratio with white Herba Lysimachiae foenumgraeci extract is 0.39：2.4：4.5.
4. composition according to claim 1, it is characterised in that the sealwort stem cell extract and dragon tree extract
Mass ratio be 0.5~1.7：6.1.
5. composition according to claim 4, it is characterised in that the sealwort stem cell extract and dragon tree extract
Mass ratio be 0.67：6.1.
6. composition according to claim 1, it is characterised in that the composition contains the composition of following parts by weight：It is yellow
Keen-witted and capable 0.39 part of cell extract, 2.4 parts of Cortex Meliae extract, 4.5 parts of white Herba Lysimachiae foenumgraeci extract, 8.2 parts of winter bamboo shoot extract and
3.55 parts of dragon tree extract.
7. a kind of anti-acne skin care item, it is characterised in that the skin care item include the composition described in any one of claim 1~6.
8. skin care item according to claim 7, it is characterised in that sealwort stem cell extract, China tree in the skin care item
Bark extract, white Herba Lysimachiae foenumgraeci extract, the quality percentage total amount of winter bamboo shoot extract and dragon tree extract are 8.1~10.3%.
9. skin care item according to claim 7, it is characterised in that the sealwort stem cell extract obtains by the following method
：
S1, sealwort new life root or branch sterilization, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain sealwort stem cell；
S4, gained sealwort stem cell obtain described sealwort stem cell extract through the micro-nano method broken wall of low temperature.
10. skin care item according to claim 7, it is characterised in that the dragon tree extract is prepared by the following：
Dragon's blood leaf obtains dragon tree extract after high speed bead mill method broken wall using water extraction；
The winter bamboo shoot extract is prepared by the following：Winter bamboo shoot pulp is taken to obtain winter bamboo shoot using alcohol extracting method after ultrasonication
Extract；
The white Herba Lysimachiae foenumgraeci extract is prepared by the following：White lavender is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains white Herba Lysimachiae foenumgraeci extract；
The Cortex Meliae extract is prepared by the following：Azedarach is taken after the micro-nano method broken wall of low temperature, using alcohol extracting method
Obtain Cortex Meliae extract.
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| CN110638728A (en) * | 2019-11-14 | 2020-01-03 | 广州一一生物技术有限公司 | Skin care composition for removing acne and lightening acne marks and preparation method and application thereof |
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