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CN107815443A - 一种呼吸链超级复合物蛋白 - Google Patents

一种呼吸链超级复合物蛋白 Download PDF

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CN107815443A
CN107815443A CN201710821771.0A CN201710821771A CN107815443A CN 107815443 A CN107815443 A CN 107815443A CN 201710821771 A CN201710821771 A CN 201710821771A CN 107815443 A CN107815443 A CN 107815443A
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杨茂君
谷金科
吴萌
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Abstract

本发明涉及一种哺乳类细胞呼吸链超级复合物蛋白，尤其是涉及具有高分辨原子分辨率冷冻电镜结构的呼吸链超级复合物蛋白，以及该蛋白的制备方法，采用该蛋白结构有助于进一步研究哺乳动物的呼吸作用机制以及与该呼吸作用相关的疾病。

Description

一种呼吸链超级复合物蛋白
技术领域
本发明涉及一种哺乳类细胞呼吸链超级复合物蛋白，尤其是具有高分辨原子分辨率冷冻电镜的结构，以及该蛋白的制备方法。
背景技术
呼吸作用发生在几乎所有的生命体中，并且随着生物进化在越高等的生物体中进行呼吸作用的蛋白复合物越复杂。在哺乳动物中，进行呼吸作用的场所是线粒体。线粒体基质中，经过三羧酸循环(TCA循环)稳定的化学能被转换成不稳定的化学能储存在NADH或FADH2中，这些分子则通过氧化呼吸链在线粒体内膜两侧形成质子梯度，而最终线粒体内膜上的ATP合成酶则利用质子梯度形成的电化学势能合成高能分子ATP。人们对形成质子梯度的氧化呼吸链的认识经历了一个漫长的过程，目前认为，氧化呼吸链由4个功能相对独立的蛋白复合物组成，即呼吸链复合物I(CI，即NADH脱氢酶复合物I)，呼吸链复合物II(CII，即琥珀酸脱氢酶复合物II)，呼吸链复合物III(CIII，即细胞色素bc1复合物III)，呼吸链复合物IV(CIV，即细胞色素c氧化酶复合物IV)。最新的研究显示，这四个蛋白复合物之间也具有相互作用，可以形成更高级的结构：呼吸链超级复合物(respiratory supercomplex)，而具有完整呼吸活性(消耗电子供体和氧气并且产生水)的呼吸链超级复合物又被称为呼吸体(respirasome)。
从20世纪90年代开始，随着X射线晶体学和结构生物学的发展，这些蛋白复合物的原子分辨率结构被相继解析，呼吸链中电子传递和能量转换的分子机理也逐渐变得清晰。在此基础上，关于线粒体内膜上呼吸链复合物组织形式的流动态模型也被大多数人所接受。
最早被解析结构的呼吸链复合物是CIV。CIV催化电子传递的最后一步，从细胞色素c中接受电子并将电子传递给氧气分子生成水，并且利用电子传递所释放的能量将质子从线粒体基质泵入线粒体膜间隙。1995年，Iwata S等提取出了原核生物中的细胞色素c氧化酶并解析了它的晶体结构，最早鉴定出两种质子通道：K(赖氨酸)通道和D(天冬氨酸)通道(Nature 1995,376:660–669)。1996年，Tsukihara T等首次从牛心线粒体中提取出了CIV，并解析了首个完整的CIV的晶体结构。
不久之后，呼吸链复合物CIII的晶体结构也被解析。CIII从还原态的泛醌QH2中得到电子，并将电子传递给细胞色素c，同时利用释放的能量将质子泵入膜间隙。1997年，XiaD等获得了牛心中CIII的部分晶体结构(Science 1997,277,60-66)。
呼吸链复合物CI是线粒体呼吸链的起点，也是结构最为复杂的蛋白。从2006年到2013年，Sazanov LA等逐步解析了原核生物中CI的完整晶体结构，揭示了CI传递电子和转运质子的核心功能。但是与哺乳动物线粒体中具有45个亚基的CI相比，原核生物中CI只有14个核心蛋白亚基。考虑到哺乳动物CI庞大的分子量和复杂的亚基组成，传统的X射线晶体学方法不适合解析其晶体结构。2014年，Hirst J等通过冷冻电镜的方法解析了牛心中CI的中等分辨率结构。
2000年，H等通过Blue-Native PAGE的方法首次检测到了牛心线粒体中超级复合物的存在(The EMBO Journal,2000,19(8),1777-1783)。Blue-Native PAGE是一种特殊的电泳方法，可用于检测分子量在1MDa以上的超级蛋白复合物，并且可以在电泳过程中保持蛋白质的生理活性和结合状态。在的电泳结果中，CI、CII、CIII和CIV的分子量分别为1MDa、120kDa、500kDa和200kDa，其中CIII是以二聚体的形式存在的。而在分子量最大的CI的条带之上，还有许多分子量更大的条带。原位酶活性检测显示，这些大分子量的条带既有CI的活性，也有CIV的活性。通过二维变性凝胶电泳检测，这些大分子量条带中同时含有CI、CIII和CIV的蛋白亚基。以上的电泳结果表明，在正常的生理条件下，线粒体中的呼吸链复合物并不是独立存在的，而是互相结合形成更高级的组织形式，超级复合物。不同的大分子量条带反应了超级复合物中复合物单体不同的结合形式，其中具有完整呼吸活性的超级复合物结合形式又被称作呼吸体。在牛心中，最主要的组合形式是I1III2IV1，其它的组合形式还包括I1III2IV2和I1III2。随后，多个研究小组又在其它多个物种中发现了不同的超级复合物的结合形式。在酵母中，没有CI的存在，超级复合物的结合形式主要为III2IV1和III2IV2；在拟南芥中，主要的超级复合物结合形式为I1III2；小鼠中超级复合物的结合形式则较为复杂，除了与牛心中相同的结合形式外，还有报道发现了结合CII的形式，即I1II1III2IV1。最新的理论认为，这些不同的结合形式在正常的生理条件下都是存在的，并且这些不同结合形式之间的比例会随着细胞状态的变化而变化，受到精细地调控，以满足不同细胞状态下特定的能量需求。
呼吸链超级复合物的功能障碍会引起诸如疾病：阿尔茨海默病又名老年痴呆、帕金森病、多发性硬化症、共济失调症、肌萎缩性脊髓侧索硬化症、嗜酸细胞型腺瘤、遗传性视神经病变、婴儿致死性线粒体病、Leigh综合征、复合物I功能减退以及糖尿病等疾病(Biochimica et Biophysica Acta,2014,1368–1379)。
知母皂甙是来源于知母(也叫毛知母，百合科，多年生草本植物)的化合物，已知有知母皂甙A-Ⅰ、A-Ⅱ、A-Ⅲ、A-Ⅳ，B-Ⅰ和B-Ⅱ(科技导报,2010，28(12)110-115)。已有研究表明知母皂甙具有抗病原微生物、降低血糖、治疗阿尔茨海默病以及用于肿瘤治疗的用途(科技导报,2010,28(12)110-115)。但是，对于知母皂甙的作用机理还不清楚。
发明内容
本发明人采用冷冻电镜技术，解析了哺乳类呼吸链超级复合物蛋白I1III2IV1的冷冻电镜三维结构，其分辨率高于4埃达到了原子分辨率水平。
本发明人提供了一种提取高纯度哺乳类呼吸链超级复合物蛋白的方法。
本发明提供了一种哺乳类呼吸链超级复合物蛋白的冷冻电镜三维结构，由一个NADH脱氢酶复合物I、两个细胞色素bc1复合物III和一个细胞色素c氧化酶复合物IV组成超级复合物I1III2IV1，超级复合物I1III2IV1长300埃，高190埃；超级复合物整体RELION(J.Struct.Biol.2012,180,519–530)软件报告分辨率高于4埃；
其中NADH脱氢酶复合物I包含如下45个蛋白亚基：
蛋白1具有SEQ ID NO:1的蛋白序列；或者与SEQ ID NO:1具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:1具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白2具有SEQ ID NO:2的蛋白序列；或者与SEQ ID NO:2具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:2具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白3具有SEQ ID NO:3的蛋白序列；或者与SEQ ID NO:3具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:3具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白4具有SEQ ID NO:4的蛋白序列；或者与SEQ ID NO:4具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:4具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白5具有SEQ ID NO:5的蛋白序列；或者与SEQ ID NO:5具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:5具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白6具有SEQ ID NO:6的蛋白序列；或者与SEQ ID NO:6具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:6具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白7具有SEQ ID NO:7的蛋白序列；或者与SEQ ID NO:7具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:7具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白8具有SEQ ID NO:8的蛋白序列；或者与SEQ ID NO:8具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:8具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白9具有SEQ ID NO:9的蛋白序列；或者与SEQ ID NO:9具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:9具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白10具有SEQ ID NO:10的蛋白序列；或者与SEQ ID NO:10具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:10具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白11具有SEQ ID NO:11的蛋白序列；或者与SEQ ID NO:11具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:11具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白12具有SEQ ID NO:12的蛋白序列；或者与SEQ ID NO:12具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:12具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白13具有SEQ ID NO:13的蛋白序列；或者与SEQ ID NO:13具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:13具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白14具有SEQ ID NO:14的蛋白序列；或者与SEQ ID NO:14具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:14具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白15具有SEQ ID NO:15的蛋白序列；或者与SEQ ID NO:15具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:15具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白16具有SEQ ID NO:16的蛋白序列；或者与SEQ ID NO:16具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:16具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白17具有SEQ ID NO:17的蛋白序列；或者与SEQ ID NO:17具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:17具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白18具有SEQ ID NO:18的蛋白序列；或者与SEQ ID NO:18具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:18具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白19具有SEQ ID NO:19的蛋白序列；或者与SEQ ID NO:19具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:19具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白20具有SEQ ID NO:20的蛋白序列；或者与SEQ ID NO:20具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:20具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白21具有SEQ ID NO:21的蛋白序列；或者与SEQ ID NO:21具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:21具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白22具有SEQ ID NO:22的蛋白序列；或者与SEQ ID NO:22具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:22具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白23具有SEQ ID NO:23的蛋白序列；或者与SEQ ID NO:23具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:23具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白24具有SEQ ID NO:24的蛋白序列；或者与SEQ ID NO:24具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:24具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白25具有SEQ ID NO:25的蛋白序列；或者与SEQ ID NO:25具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:25具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白26具有SEQ ID NO:26的蛋白序列；或者与SEQ ID NO:26具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:26具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白27具有SEQ ID NO:27的蛋白序列；或者与SEQ ID NO:27具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:27具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白28具有SEQ ID NO:28的蛋白序列；或者与SEQ ID NO:28具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:28具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白29具有SEQ ID NO:29的蛋白序列；或者与SEQ ID NO:29具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:29具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白30具有SEQ ID NO:30的蛋白序列；或者与SEQ ID NO:30具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:30具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白31具有SEQ ID NO:31的蛋白序列；或者与SEQ ID NO:31具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:31具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白32具有SEQ ID NO:32的蛋白序列；或者与SEQ ID NO:32具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:32具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白33具有SEQ ID NO:33的蛋白序列；或者与SEQ ID NO:33具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:33具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白34具有SEQ ID NO:34的蛋白序列；或者与SEQ ID NO:34具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:34具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白35具有SEQ ID NO:35的蛋白序列；或者与SEQ ID NO:35具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:35具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白36具有SEQ ID NO:36的蛋白序列；或者与SEQ ID NO:36具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:36具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白37具有SEQ ID NO:37的蛋白序列；或者与SEQ ID NO:37具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:37具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白38具有SEQ ID NO:38的蛋白序列；或者与SEQ ID NO:38具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:38具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白39具有SEQ ID NO:39的蛋白序列；或者与SEQ ID NO:39具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:39具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白40具有SEQ ID NO:40的蛋白序列；或者与SEQ ID NO:40具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:40具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白41具有SEQ ID NO:41的蛋白序列；或者与SEQ ID NO:41具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:41具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白42具有SEQ ID NO:42的蛋白序列；或者与SEQ ID NO:42具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:42具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白43具有SEQ ID NO:43的蛋白序列；或者与SEQ ID NO:43具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:43具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白44具有SEQ ID NO:44的蛋白序列；或者与SEQ ID NO:44具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:44具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；和
蛋白45具有SEQ ID NO:45的蛋白序列；或者与SEQ ID NO:45具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:45具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
其中细胞色素bc1复合物III包含如下11个蛋白序列(本发明的超级复合物包含两个相同的细胞色素bc1复合物III，每一个细胞色素bc1复合物III都包含如下11个蛋白序列)：
蛋白46具有SEQ ID NO:46的蛋白序列；或者与SEQ ID NO:46具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:46具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白47具有SEQ ID NO:47的蛋白序列；或者与SEQ ID NO:47具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:47具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白48具有SEQ ID NO:48的蛋白序列；或者与SEQ ID NO:48具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:48具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白49具有SEQ ID NO:49的蛋白序列；或者与SEQ ID NO:49具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:49具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白50具有SEQ ID NO:50的蛋白序列；或者与SEQ ID NO:50具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:50具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白51具有SEQ ID NO:51的蛋白序列；或者与SEQ ID NO:51具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:51具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白52具有SEQ ID NO:52的蛋白序列；或者与SEQ ID NO:52具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:52具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白53具有SEQ ID NO:53的蛋白序列；或者与SEQ ID NO:53具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:53具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白54具有SEQ ID NO:54的蛋白序列；或者与SEQ ID NO:54具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:54具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白55具有SEQ ID NO:55的蛋白序列；或者与SEQ ID NO:55具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:55具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白56具有SEQ ID NO:56的蛋白序列；或者与SEQ ID NO:56具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:56具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
其中细胞色素c氧化酶复合物IV包含如下13个蛋白序列：
蛋白57具有SEQ ID NO:57的蛋白序列；或者与SEQ ID NO:57具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:57具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白58具有SEQ ID NO:58的蛋白序列；或者与SEQ ID NO:58具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:58具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白59具有SEQ ID NO:59的蛋白序列；或者与SEQ ID NO:59具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:59具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白60具有SEQ ID NO:60的蛋白序列；或者与SEQ ID NO:60具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:60具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白61具有SEQ ID NO:61的蛋白序列；或者与SEQ ID NO:61具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:61具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白62具有SEQ ID NO:62的蛋白序列；或者与SEQ ID NO:62具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:62具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白63具有SEQ ID NO:63的蛋白序列；或者与SEQ ID NO:63具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:63具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白64具有SEQ ID NO:64的蛋白序列；或者与SEQ ID NO:64具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:64具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白65具有SEQ ID NO:65的蛋白序列；或者与SEQ ID NO:65具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:65具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白66具有SEQ ID NO:66的蛋白序列；或者与SEQ ID NO:66具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:66具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白67具有SEQ ID NO:67的蛋白序列；或者与SEQ ID NO:67具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:67具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白68具有SEQ ID NO:68的蛋白序列；或者与SEQ ID NO:68具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:68具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；和
蛋白69具有SEQ ID NO:69的蛋白序列；或者与SEQ ID NO:69具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:69具有一个或几个氨基酸的取代、缺失或替换的蛋白序列。
在优选的实施方式中，本发明的蛋白冷冻电镜三维结构中还包含1个FMN分子(位于NDUFV1亚基)、10个铁硫簇、一个NADPH分子(位于NDUFA9亚基)、一个锌离子(位于NDUFA6亚基)、3个磷脂酰胆碱(散布于复合物I的跨膜区)、4个磷脂酰乙醇胺(散布于复合物I的跨膜区)、4个心磷脂(散布于复合物I的跨膜区)、2个细胞色素bL(位于复合物III的CYTB亚基)、2个细胞色素bH(位于复合物III的CYTB亚基)、两个细胞色素c1(位于位于复合物III的CYT1亚基)、CuA中心(位于复合物IV COX2亚基)、CuB中心位于(位于复合物IV COX1亚基)、1个血红素a(位于复合物IV COX1亚基)和1个血红素a3(位于复合物IV COX1亚基)。
在优选的实施方式中，本发明蛋白，其中NADH脱氢酶复合物I包含如下45个蛋白质序列：SEQ ID NO:1(即NDUFS2亚基),SEQ ID NO:2(即NDUFS3亚基)，SEQ ID NO:3(即NDUFV2亚基),SEQ ID NO:4(即MT-ND1亚基),SEQ ID NO:5(即NDUFS7亚基),SEQ ID NO:6(即NDUFB8亚基),SEQ ID NO:7(即NDUFA6亚基),SEQ ID NO:8(即NDUFAB1a亚基),SEQ ID NO:9(即NDUFAB1b亚基),SEQ ID NO:10(即NDUFS4亚基),SEQ ID NO:11(即NDUFA2亚基),SEQ IDNO:12(即NDUFA1亚基),SEQ ID NO:13(即NDUFC1亚基),SEQ ID NO:14(即ND4L亚基),SEQID NO:15(即NDUFS8亚基),SEQ ID NO:16(即ND2亚基),SEQ ID NO:17(即ND3亚基),SEQ IDNO:18(即ND4亚基),SEQ ID NO:19(即ND5亚基),SEQ ID NO:20(即ND6亚基),SEQ ID NO:21(即NDUFS1亚基)，SEQ ID NO:22(即NDUFS5亚基),SEQ ID NO:23(即NDUFS6亚基)，SEQ IDNO:24(即NDUFV1亚基),SEQ ID NO:25(即NDUFA3亚基),SEQ ID NO:26(即NDUFA5亚基)，SEQID NO:27(即NDUFA7亚基),SEQ ID NO:28(即NDUFA8亚基),SEQ ID NO:29(即NDUFA9亚基),SEQ ID NO:30(即NDUFA10亚基),SEQ ID NO:31(即NDUFA11亚基),SEQ ID NO:32(即NDUFA12亚基),SEQ ID NO:33(即NDUFA13亚基),SEQ ID NO:34(即NDUFB1亚基),SEQ IDNO:35(即NDUFB2亚基),SEQ ID NO:36(即NDUFB3亚基),SEQ ID NO:37(即NDUFB4亚基),SEQID NO:38(即NDUFB5亚基),SEQ ID NO:39(即NDUFB6亚基),SEQ ID NO:40(即NDUFB7亚基),SEQ ID NO:41(即NDUFB9亚基),SEQ ID NO:42(即NDUFB10亚基)，SEQ ID NO:43(即NDUFB11亚基)和SEQ ID NO:44(即NDUFC2亚基)，和SEQ ID NO:45(即NDUFV3亚基)；
细胞色素bc1复合物III包含如下11个蛋白质序列：SEQ ID NO:46(即UQCRFS1亚基)，SEQ ID NO:47(即MT-CYB亚基),SEQ ID NO:48(即CYC1亚基),SEQ ID NO:49(即UQCRC1亚基),SEQ ID NO:50(即UQCRC2亚基),SEQ ID NO:51(即UQCRH亚基),SEQ ID NO:52(即UQCRB亚基),SEQ ID NO:53(即UQCRQ亚基),SEQ ID NO:54(即UQCR10亚基),SEQ ID NO:55(即UQCR11亚基),和SEQ ID NO:56(即UQCRFS1信号肽亚基)；
细胞色素c氧化酶复合物IV包含如下13个蛋白质序列：SEQ ID NO:57(即MT-CO3亚基),SEQ ID NO:58(即MT-CO1亚基),SEQ ID NO:59(即MT-CO2亚基),SEQ ID NO:60(即COX4亚基),SEQ ID NO:61(即COX5A亚基)，SEQ ID NO:62(即COX5B亚基),SEQ ID NO:63(即COX6A亚基)，SEQ ID NO:64(即COX6B亚基),SEQ ID NO:65(即COX6C亚基)和SEQ ID NO:66(即COX7A亚基)SEQ ID NO:67(即COX7B亚基),SEQ ID NO:68(即COX7C亚基),SEQ ID NO:69(即COX8H亚基)。
在一个优选的实施方案中，本发明的呼吸链超级复合物蛋白由80个蛋白序列组成，由于细胞色素bc1复合物III有2个，因此该80个蛋白序列为上述SEQ ID NOs：1-69以及另一个细胞色素bc1复合物III的序列SEQ ID NOs:46-56。优选地，本发明所述的NADH脱氢酶复合物I为哺乳动物来源，尤其是来源于猪(Susscrofa)。
在进一步优选的实施方案中，本发明的蛋白冷冻电镜三维结构采用原子坐标来限定。
本发明从猪心线粒体中提取超级复合物，其结合形式与牛心中基本一致，最主要的形式为I1III2IV1，也就是呼吸体。通过冷冻电镜单颗粒三维重构的方法，我们研究组在全世界范围内首次拿到了呼吸体的高分辨率蛋白结构。在我们的结构中，不仅清晰地解析了单独的CI和CIII的原子分辨率结构，而且精确地确定了CI、CIII和CIV之间的相对位置以及它们之间发生相互作用的蛋白亚基。我们的结构为建立全新的电子传递和底物通道理论奠定了坚实的基础，让我们可以更为深入地理解呼吸体中能量转换的机制。
CI是呼吸体中最大的单体，亲水臂和跨膜臂连接呈L形。与CI单独存在时其跨膜臂并呈一条直线不同，在呼吸体中CI的跨膜臂在膜平面内略微弯曲呈弧形，其亲水臂也更加靠近中部。CIII以二聚体的形式在呼吸体中存在，结合在CI跨膜臂的凹面，二聚体中的一个单体面向CI，而另一个单体指向呼吸体之外。这一结构上的差异暗示了二者在功能上是有区别的。CIV结合在CI跨膜臂的远端，也就是说CI的跨膜臂一端连接亲水臂，而另一端与CIV结合。由于CI跨膜臂呈弧形，CIV与CIII之间的相对位置非常接近，使它们之间的相互作用成为可能。CI、CIII和CIV的跨膜区位于同一个平面，相互靠近构成一个盘状结构。CI与CIII之间的相互作用非常紧密。在跨膜区内，CI中的蛋白亚基NDUFB4、NDUFB8和NDUFA11可以与CIII中的蛋白亚基UQCRB和UQCRQ发生相互作用，同时，由于CI非常庞大，处于跨膜区中段的NDUFA11在CI自身的组装过程中也发挥着非常巨大的作用，NDUFA11主要与CI的ND5、ND2、ND4和NDUFB5亚基相互作用，使得NDUFA11与CI紧密相连成为其重要的组成部分。另外，CI中的NDUFB4、NDUFB9还与CIII中的UQCRC1发生相互作用，进一步加强CI与CIII之间的联系。并且，磷脂酰胆碱分子也会介导复合物I的NDUFA11,NDUFB4,ND5同复合物III UQCRQ相互作用。CIV与CI和CIII之间的相互作用则相对较弱。CIV中的COX7C与CI中的ND5有相互作用，NDUFB3,NDUFB7和NDUFB8同复合物IV的COX7C和COX8在空间位置上相互靠近。同时，CIV中的COX7A则与CIII中的UQCRC1、UQCR11和UQCRB都有相互作用。在CI与CIII的跨膜区之间有一个缝隙，我们认为会被线粒体内膜中的脂质填充，同时，也是辅酶Q储存和流动的场所。辅酶Q被限制在这一区域会极大地提高CI与CIII之间电子传递的效率。CIII和CIV结合细胞色素c的位点都位于线粒体膜间隙，在呼吸体中这两个位点处于同一平面且非常接近，这将促进CIII与CIV之间细胞色素c的传递。
本发明还涉及一种制备所述呼吸链超级复合物蛋白的方法，该方法包括以下步骤：A.线粒体的提取；B.从线粒体中抽提呼吸链超级复合物蛋白；C.纯化步骤B获得的超级复合物蛋白；D.对步骤C的纯化产物进行BN-PAGE检测；和E.进行凝胶排阻层析。
在优选的实施方案中，步骤B采用洋地黄皂苷来抽提呼吸链超级复合物蛋白。
在优选的实施方案中，在步骤E中，以三羟基氨基甲烷、氯化钾和洋地黄皂苷为溶质配制层析缓冲液，将含有超级复合物的样品载入到凝胶排阻层析柱上，进行层析，并收取峰尖。优选层析缓冲液具有7-8的pH值，进一步优选为7.3-7.5的pH值。优选地，其中所述的峰尖是采用的AKTA purifier层析仪器，层析柱为Superose 6 10/300GL，收集在280nm波长下检测到的蛋白峰的最高值。
在优选的实施方案中，呼吸链超级复合物蛋白是来源于人或猪。
附图说明
图1为哺乳类来源的超级复合物I1III2IV1的三维冷冻电镜结构
A为冷冻电镜得到的呼吸链超级复合物的密度图，各个复合物均已表明；
B为密度图和卡通结构图放在一起的得到的整体结构图；
C为超级复合物整体结构的模型。
图2为哺乳类来源超级复合物I1III2IV1中复合体I与复合物III相互作用关系。
A为位于复合物I和III之间介导两者相互作用的NDUFA11,NDUFB4和NDUFB8的位置示意图。
B为NDUFA11一侧与复合物I的ND5亚基相互作用，具体作用示意图。
C为NDUFA11C端同复合物I的ND2,ND4和NDUFB5的作用关系示意图。
D为介导复合物I的NDUFA11,NDUFB4,ND5同复合物III UQCRQ相互作用的磷脂酰胆碱示意图。
E为复合物I NDUFA11同复合物III相互作用示意图。
F为复合物III的UQCRC1同复合物I的NDUFB4、NDUFB9作用示意图。
图3为哺乳类来源超级复合物I1III2IV1中复合体IV与复合物III或复合物I相互作用关系。
A为复合物IV同复合物I的相互作用关系，复合物I的ND5亚基503位的谷氨酸同复合物IV的COX7C亚基20位的精氨酸形成氢键。此外复合物I的NDUFB3,NDUFB7和NDUFB8同复合物IV的COX7C和COX8在空间位置上相互靠近。
B为复合物IV同复合物III的相互作用关系。复合物IVCOX7A同复合物III的UQCRC1,UQCR11的N端和UQCRB的C端相互作用。
图4为电镜原始数据。
图5为电镜数据处理流程。
发明详述
蛋白
“多肽”、“蛋白”和“亚基”在本文中含义相同，都是指氨基酸残基的聚合物。氨基酸残基可以是天然产物或者是化学类似物。
呼吸链复合物蛋白
呼吸链复合物蛋白是指锚定在线粒体内膜上，行使将电子从NADH或FADH2传递给氧气，并使得线粒体内膜两侧产生质子梯度的多亚基蛋白复合物。总共包含四种复合物，分别是：呼吸链复合物I(CI，NADH脱氢酶)，呼吸链复合物II(CII，琥珀酸脱氢酶)，呼吸链复合物III(CIII，细胞色素bc1复合体)，呼吸链复合物IV(CIV，细胞色素c氧化酶)。
同一性
由参数“同一性”描述两个氨基酸序列之间的相关性。
就本发明而言，两个氨基酸序列之间的同一性程度使用如EMBOSS软件包(EMBOSS：The European Molecular Biology Open Software Suite，Rice等，2000，Trends inGenetics 16：276-277)的Needle程序，优选3.0.0版或更高版本中执行的Needleman-Wunsch算法(Needleman和Wunsch，1970，J.Mol.Biol.48：443-453)来确定。使用的任选参数为缺口罚分(gap penalty)10，缺口延伸罚分(gap extension penalty)0.5和EBLOSUM62取代矩阵(BLOSUM62的EMBOSS版)。使用Needle标记为“最高同一性(longest identity)”(使用-nobrief选项获得)的输出结果作为百分比同一性，并计算如下：
(相同的残基×100)/(比对长度-比对中缺口的总数)。
原子坐标
“原子坐标”或“结构坐标”指描述原子在Protein Data Bank(PDB)格式(format)中位置的数学坐标，包括每个原子的X、Y、Z和B坐标。本领域技术人员应理解，由X射线结晶学确定的一组结构坐标并不是没有标准偏差的。在本发明中，任何来源的线粒体呼吸链超级复合物I1III2IV1结构坐标，当叠加在原子坐标的非氢原子位置上时，其非氢原子位置的均方根偏差小于即认为它们均有实质同一性或同源性。在更优选的实施方案中，任何来源的线粒体呼吸链超级复合物I1III2IV1的任何组的结构坐标，当叠加在原子坐标的非氢原子位置上时，其非氢原子位置的均方根偏差小于即认为它们均有实质同一性或同源性。
实施例
实施例1.呼吸链超级复合物蛋白I1III2IV1的纯化
线粒体的提取
100g新鲜猪心组织，切成2cm×2cm×2cm小块，用100mL缓冲液A(100mM Tris pH7.4,225mM山梨醇,60mM KCl,1mM EGTA和0.1％牛血清蛋白)悬起。用大容量组织匀浆机破碎300秒。将匀浆液转移至50mL离心管中，3000g离心10分钟(min)，弃去细胞碎片等沉淀，留取上清。将上清转移至高速离心管中，20,000离心30分钟，得到沉淀，此沉淀即为粗提的线粒体。配制缓冲液B(100mM Tris pH 7.4,250mM蔗糖,60mM KCl,40％Percoll(经过聚乙烯吡咯烷酮处理的硅胶颗粒混悬液)和0.1mM EGTA)，用100mL缓冲液B重悬粗提的线粒体，60,000离心50分钟，而后会形成明晰的线粒体层，用吸管吸取悬浮的线粒体，加入到缓冲液C(100mM Tris pH7.4,100mM山梨醇,60mM KCl,0.05mM EGTA)中。将重悬液20,000g离心30分钟，得到的沉淀即为超纯线粒体。
超级复合物蛋白从线粒体中抽提
将提纯的线粒体用缓冲液C重悬，然后加入终浓度为1％的洋地黄皂苷(digitonin)，放置在旋转摇床上4℃抽提过夜。收取抽提液，150,000g离心30分钟。收取上清，将上清加入到100kDa超滤浓缩管中浓缩至1mL。
超级复合物蛋白的纯化
配制缓冲液D(25mM Tris,pH 7.4,60mM KCl,0.1％洋地黄皂苷，0.3M蔗糖)和缓冲液E(25mM Tris,pH 7.4,60mM KCl,0.1％洋地黄皂苷，1.8M蔗糖)，用两种缓冲液配制成11mL连续的0.3M-1.8M的浓度梯度，装载到SW41转子专用的离心管中，而后将1mL超级复合物粗提蛋白轻轻加到11mL连续梯度之上，采用SW41转子，150,000g，4℃，离心20小时。
BN-PAGE检测
将离心完毕的离心管轻轻取出，1mL一管收取样品，分别制样，进行3％-10％梯度BN-PAGE电泳，检测哪些样品中含有超级复合物。
凝胶排阻层析
以10mM Tris pH7.4,60mM KCl,0.1％洋地黄皂苷为层析缓冲液，将含有超级复合物的样品载入到凝胶排阻层析柱Superose 6 10/300GL上，进行层析，收取峰尖，此为超纯的超级复合物。
小子药物添加与浸泡
在提纯的超级复合物中添加终浓度1μM的知母皂甙，4℃孵育3小时。
实施例2.呼吸链超级复合物蛋白I1III2IV1冷冻电镜数据的收集及蛋白结构解析
电镜样品制备
取4μL浸泡过小分子药物的超级复合物蛋白滴加到事先铺好碳膜的400目Quantifoil R1.2/1.3铜网(购自Quantifoil,Micro Tools GmbH,Germany)上，静置1.5秒。然后用FEI Mark IV Vitrobot制样机器制取冷冻样品。
电镜数据采集
采用300kVTitan Krios进行高分辨率图像数据的收集，检测器为Falcon IIdirect electron detector(FEI公司)。数据采集的控制软件为EPU(FEI公司)。电镜的放大倍数是75,000×，像素大小为1.08埃，欠焦值为-1.3μm到-2.3μm，电子剂量为35个/像素/秒，曝光时间为1.5秒，每个显微照片栈包含28幅照片。
结果见图4.电镜原始数据，图5为电镜数据处理流程
电镜图像处理
图像漂移校正采用dosefgpu_driftcorr program(2013，Nature Methods 10,584–590.)。使用EMAN2.1(2007,J.Struct.Biol.157,38–46)和RELION1.4(2012,J.Struct.Biol.180,519–530)这两个程序进行图像筛选、单颗粒挑选、归一化。使用CTFFIND3(2003,J.Struct.Biol.142,334–347)进行衬度传递函数(CTF)校正。单颗粒的二维、三维分类以及优化是用RELION程序完成的。从4660张图片中挖出的424,002个单颗粒分子，进行一系列二维和三维分类，而后161,912个相对比较均一的单颗粒分子被拿来用作最终的优化。通过局部优化以及加入各种柔性表面限定模型(soft mask)，最终的得到3.6埃分辨率的复合体I的电镜数据，3.97埃超级复合物I1III2IV1的电镜数据，3.9埃超级复合物I1III2的电镜数据，3.6埃复合体III的电镜数据，7.5埃复合体IV的电镜数据。最终报告分辨率基于FSC＝0.143的标准。最终的电子云密度图采用的标准，使用RELION程序进行处理。局部分辨率图使用ResMap(2014,Nature Methods 11,63–65)程序生成。
三维结构模型的搭建及优化
采用5.0埃低分辨率的牛心来源的复合体I(pdb号4UQ8)为初始模型，通过COOT软件(2004,Acta Crystallogr.D 60,2126–2132)，以3.6埃的电子云密度图为基础，将各氨基酸的侧链搭进密度图并进行位置的优化。复合物I的14个核心亚基以及30个附属亚基搭建进了3.6埃的电子云密度图。对于超级复合物中原子分辨率的复合体III结构的解析，采用pdb号为1BGY X-ray结构作为初始模型，采用COOT软件进行模型搭建及修正。磷脂三维结构通过PHENIX(2010,Acta Cryst.D66,213-221)的elBOW组件以simile文件生成。最后，所有的小分子以及蛋白的整体结构通过PHENIX进行整体优化。最终得到完整的超级复合物I1III2IV1以及各组分的结构模型。
SEQUENCE LISTING
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<213> Sus scrofa
<400> 10
Ala Gln Asp Gln Thr Gln Asp Thr Gln Leu Ile Ala Val Asp Glu Lys
1 5 10 15
Leu Asp Ile Thr Thr Leu Thr Gly Val Pro Glu Glu His Ile Lys Thr
20 25 30
Arg Lys Val Arg Ile Phe Val Pro Ala Arg Asn Asn Met Gln Ser Gly
35 40 45
Val Asn Asn Thr Lys Lys Trp Lys Met Glu Phe Asp Thr Arg Glu Arg
50 55 60
Trp Glu Asn Pro Leu Met Gly Trp Ser Ser Thr Ala Asp Pro Leu Ser
65 70 75 80
Asn Leu Val Leu Thr Phe Ser Thr Lys Glu Asp Ala Val Ala Phe Ala
85 90 95
Glu Lys Asn Gly Trp Ser Phe Asp Val Glu Glu Arg Lys Val Pro Lys
100 105 110
Pro Lys Ser Lys Ser Tyr Gly Ala Asn Phe Ser Trp Asn Lys Arg Thr
115 120 125
Arg Val Ser Thr Lys
130
<210> 11
<211> 98
<212> PRT
<213> Sus scrofa
<400> 11
Ala Ala Ala Ala Ala Ser Arg Gly Ile Arg Ala Lys Leu Gly Leu Arg
1 5 10 15
Glu Ile Arg Ile His Leu Cys Gln Arg Ser Pro Gly Ser Gln Gly Val
20 25 30
Arg Asp Phe Ile Glu Lys Arg Tyr Val Glu Leu Lys Lys Ala Asn Pro
35 40 45
Asp Leu Pro Ile Leu Ile Arg Glu Cys Ser Asp Val Gln Pro Lys Leu
50 55 60
Trp Ala Arg Tyr Ala Phe Gly Gln Glu Lys Asn Val Ser Leu Asn Asn
65 70 75 80
Phe Ser Ala Asp Gln Val Thr Arg Thr Leu Glu Asn Val Leu Ser Gly
85 90 95
Lys Ala
<210> 12
<211> 70
<212> PRT
<213> Sus scrofa
<400> 12
Met Trp Phe Glu Ile Leu Pro Gly Ile Ala Val Met Ala Ala Cys Leu
1 5 10 15
Phe Ile Pro Gly Met Ala Thr Ala His Ile His Lys Phe Thr Asn Gly
20 25 30
Gly Lys Glu Lys Arg Val Ala His Phe Ser Tyr Gln Trp Asn Leu Met
35 40 45
Glu Arg Asp Arg Arg Ile Ser Gly Val Asn Arg Tyr His Val Thr Lys
50 55 60
Gly Leu Glu Asn Ile Asp
65 70
<210> 13
<211> 49
<212> PRT
<213> Sus scrofa
<400> 13
Lys Phe Tyr Ile Arg Glu Pro Pro His Gly Ser Pro Asp Trp Leu Lys
1 5 10 15
Val Gly Leu Thr Leu Gly Thr Ser Val Phe Leu Trp Ile Tyr Leu Ile
20 25 30
Lys Gln His Lys Glu Asp Val Leu Glu Tyr Lys Arg Arg Asn Gly Leu
35 40 45
Glu
<210> 14
<211> 98
<212> PRT
<213> Sus scrofa
<400> 14
Met Pro Leu Val Tyr Met Asn Ile Ile Met Ala Phe Ala Ile Ala Leu
1 5 10 15
Ala Gly Leu Leu Met Tyr Arg Ser His Leu Met Ser Ser Leu Leu Cys
20 25 30
Leu Glu Gly Met Met Leu Ser Leu Phe Ile Met Ser Thr Leu Ile Ile
35 40 45
Leu Asn Thr His Phe Thr Leu Ala Asn Met Met Pro Ile Ile Leu Leu
50 55 60
Val Phe Ala Ala Cys Glu Ala Ala Leu Gly Leu Ser Leu Leu Val Met
65 70 75 80
Val Ser Asn Thr Tyr Gly Thr Asp Tyr Val Gln Asn Leu Asn Leu Leu
85 90 95
Gln Cys
<210> 15
<211> 176
<212> PRT
<213> Sus scrofa
<400> 15
Thr Tyr Lys Phe Val Asn Met Arg Glu Pro Ser Met Asp Met Lys Ser
1 5 10 15
Val Thr Asp Arg Ala Ala Gln Thr Leu Leu Trp Thr Glu Leu Val Arg
20 25 30
Gly Leu Gly Met Thr Leu Ser Tyr Leu Phe Arg Glu Pro Ala Thr Ile
35 40 45
Asn Tyr Pro Phe Glu Lys Gly Pro Leu Ser Pro Arg Phe Arg Gly Glu
50 55 60
His Ala Leu Arg Arg Tyr Pro Ser Gly Glu Glu Arg Cys Ile Ala Cys
65 70 75 80
Lys Leu Cys Glu Ala Val Cys Pro Ala Gln Ala Ile Thr Ile Glu Ala
85 90 95
Glu Pro Arg Ala Asp Gly Ser Arg Arg Thr Thr Arg Tyr Asp Ile Asp
100 105 110
Met Thr Lys Cys Ile Tyr Cys Gly Phe Cys Gln Glu Ala Cys Pro Val
115 120 125
Asp Ala Ile Val Glu Gly Pro Asn Phe Glu Phe Ser Thr Glu Thr His
130 135 140
Glu Glu Leu Leu Tyr Asn Lys Glu Lys Leu Leu Asn Asn Gly Asp Lys
145 150 155 160
Trp Glu Ala Glu Ile Ala Ala Asn Ile Gln Ala Asp Tyr Leu Tyr Arg
165 170 175
<210> 16
<211> 347
<212> PRT
<213> Sus scrofa
<400> 16
Met Asn Pro Ile Ile Tyr Thr Thr Leu Ile Met Thr Val Met Ser Gly
1 5 10 15
Thr Met Leu Val Met Ile Ser Ser His Trp Leu Leu Ile Trp Ile Gly
20 25 30
Phe Glu Met Asn Leu Leu Ala Met Ile Pro Val Leu Met Lys Asn Phe
35 40 45
Asn Pro Arg Ala Thr Glu Ala Ala Thr Lys Tyr Phe Leu Thr Gln Ala
50 55 60
Thr Ala Ser Met Met Leu Met Met Ala Ile Ile Ile Asn Leu Leu Tyr
65 70 75 80
Ser Gly Gln Trp Thr Ile Thr Lys Met Phe Asn Pro Val Ala Met Thr
85 90 95
Met Met Thr Met Ala Leu Ala Met Lys Leu Gly Leu Ser Pro Phe His
100 105 110
Phe Trp Val Pro Glu Val Thr Gln Gly Ile Ser Leu Gln Ala Gly Leu
115 120 125
Leu Leu Leu Thr Trp Gln Lys Leu Ala Pro Leu Ser Val Leu Cys Gln
130 135 140
Ile Ser Gln Ser Ile Asn Pro Asn Leu Met Leu Thr Met Ala Met Leu
145 150 155 160
Ser Ile Leu Ile Gly Gly Trp Gly Gly Leu Asn Gln Thr Gln Leu Arg
165 170 175
Lys Ile Met Ala Tyr Ser Ser Ile Ala His Met Gly Trp Met Thr Ala
180 185 190
Val Leu Pro Tyr Asn Thr Thr Met Thr Ile Leu Asn Leu Leu Ile Tyr
195 200 205
Ile Thr Met Thr Leu Ala Met Phe Met Leu Leu Ile His Ser Ser Ala
210 215 220
Thr Thr Thr Leu Ser Leu Ser His Thr Trp Asn Lys Met Pro Val Ile
225 230 235 240
Thr Ser Leu Met Met Val Thr Leu Leu Ser Met Gly Gly Leu Pro Pro
245 250 255
Leu Ser Gly Phe Met Pro Lys Trp Met Ile Ile Gln Glu Met Thr Lys
260 265 270
Asn Glu Ser Ile Ile Met Pro Thr Leu Met Ala Met Thr Ala Leu Leu
275 280 285
Asn Leu Tyr Phe Tyr Met Arg Leu Ala Tyr Ser Ser Ser Leu Thr Met
290 295 300
Phe Pro Ser Thr Asn Asn Met Lys Met Lys Trp Gln Phe Glu His Thr
305 310 315 320
Lys Gln Met Lys Leu Leu Pro Thr Met Ile Val Leu Ser Thr Leu Val
325 330 335
Leu Pro Met Thr Pro Ala Leu Ser Ser Leu Asn
340 345
<210> 17
<211> 115
<212> PRT
<213> Sus scrofa
<400> 17
Met Asn Ile Met Leu Thr Leu Leu Thr Asn Val Thr Leu Ala Ser Leu
1 5 10 15
Leu Val Leu Ile Ala Phe Trp Leu Pro Gln Leu Asn Ala Tyr Ser Glu
20 25 30
Lys Thr Ser Pro Tyr Glu Cys Gly Phe Asp Pro Met Gly Ser Ala Arg
35 40 45
Leu Pro Phe Ser Met Lys Phe Phe Leu Val Ala Ile Thr Phe Leu Leu
50 55 60
Phe Asp Leu Glu Ile Ala Leu Leu Leu Pro Leu Pro Trp Ala Ser Gln
65 70 75 80
Thr Asn Asn Leu Lys Thr Met Leu Thr Met Ala Leu Phe Leu Leu Ile
85 90 95
Leu Leu Ala Ala Ser Leu Ala Tyr Glu Trp Thr Gln Lys Gly Leu Glu
100 105 110
Trp Ala Glu
115
<210> 18
<211> 459
<212> PRT
<213> Sus scrofa
<400> 18
Met Leu Lys Ile Ile Ile Pro Thr Thr Met Leu Leu Pro Met Thr Trp
1 5 10 15
Met Ser Lys His Asn Met Ile Trp Ile Asn Ala Thr Val His Ser Leu
20 25 30
Leu Ile Ser Leu Ile Ser Leu Ser Leu Leu Asn Gln Leu Gly Glu Asn
35 40 45
Ser Leu Asn Phe Ser Leu Thr Phe Phe Ser Asp Ser Leu Ser Ala Pro
50 55 60
Leu Leu Val Leu Thr Thr Trp Leu Leu Pro Leu Met Leu Met Ala Ser
65 70 75 80
Gln Ser His Leu Ser Lys Glu Thr Thr Thr Arg Lys Lys Leu Tyr Ile
85 90 95
Thr Met Leu Ile Leu Leu Gln Leu Phe Leu Ile Met Thr Phe Thr Ala
100 105 110
Thr Glu Leu Ile Leu Phe Tyr Ile Leu Phe Glu Ala Thr Leu Val Pro
115 120 125
Thr Leu Ile Ile Ile Thr Arg Trp Gly Asn Gln Thr Glu Arg Leu Asn
130 135 140
Ala Gly Leu Tyr Phe Leu Phe Tyr Thr Leu Ala Gly Ser Leu Pro Leu
145 150 155 160
Leu Val Ala Leu Val Tyr Ile Gln Asn Thr Thr Gly Ser Leu Asn Phe
165 170 175
Leu Ile Ile His Tyr Trp Ser His Pro Leu Ser Asn Ser Trp Ser Asn
180 185 190
Ile Phe Met Trp Leu Ala Cys Ile Met Ala Phe Met Val Lys Met Pro
195 200 205
Leu Tyr Gly Leu His Leu Trp Leu Pro Lys Ala His Val Glu Ala Pro
210 215 220
Ile Ala Gly Ser Met Val Leu Ala Ala Val Leu Leu Lys Leu Gly Gly
225 230 235 240
Tyr Gly Met Met Arg Ile Thr Thr Ile Leu Asn Pro Leu Thr Asn Tyr
245 250 255
Met Ala Tyr Pro Phe Leu Met Leu Ser Met Trp Gly Met Ile Met Thr
260 265 270
Ser Ser Ile Cys Leu Arg Gln Thr Asp Leu Lys Ser Leu Ile Ala Tyr
275 280 285
Ser Ser Val Ser His Met Ala Leu Val Ile Val Ala Ile Met Ile Gln
290 295 300
Thr Pro Trp Ser Phe Met Gly Ala Thr Ala Leu Met Ile Ala His Gly
305 310 315 320
Leu Thr Ser Ser Met Leu Phe Cys Leu Ala Asn Thr Asn Tyr Glu Arg
325 330 335
Val His Ser Arg Thr Met Ile Leu Ala Arg Gly Leu Gln Thr Leu Leu
340 345 350
Pro Leu Met Ala Thr Trp Trp Leu Met Ala Ser Leu Thr Asn Leu Ala
355 360 365
Leu Pro Pro Ser Ile Asn Leu Ile Gly Glu Leu Phe Ile Ile Thr Ala
370 375 380
Ser Phe Ser Trp Ser Asn Ile Thr Ile Ile Leu Met Gly Met Asn Met
385 390 395 400
Met Ile Thr Ala Leu Tyr Ser Leu Tyr Met Leu Ile Thr Thr Gln Arg
405 410 415
Gly Lys Tyr Thr His His Ile Asn Asn Ile Lys Ala Ser Phe Thr Arg
420 425 430
Glu Asn Ala Leu Met Ala Leu His Ile Leu Pro Leu Leu Leu Leu Thr
435 440 445
Leu Asn Pro Lys Met Ile Leu Gly Pro Leu Tyr
450 455
<210> 19
<211> 606
<212> PRT
<213> Sus scrofa
<400> 19
Met Asn Pro Phe Ala Ser Leu Thr Leu Thr Thr Leu Ile Ile Leu Thr
1 5 10 15
Ile Pro Ile Met Met Ser Asn Ser Asn Ile Tyr Lys Thr Asn Leu Tyr
20 25 30
Pro Asn Tyr Val Lys Thr Thr Val Ser Tyr Ala Phe Thr Leu Ser Leu
35 40 45
Val Pro Leu Leu Met Phe Met His Thr Gly Gln Glu Met Ile Ile Ser
50 55 60
Asn Trp His Trp Met Thr Leu Gln Thr Val Glu Leu Ser Leu Ser Phe
65 70 75 80
Lys Met Asp Tyr Phe Ser Val Met Phe Ile Pro Val Ala Leu Phe Val
85 90 95
Thr Trp Ser Ile Met Glu Phe Ser Met Trp Tyr Met His Ser Asp Pro
100 105 110
Phe Ile Asn Arg Phe Phe Lys Tyr Leu Val Leu Phe Leu Ile Thr Met
115 120 125
Met Ile Leu Val Thr Ala Asn Asn Leu Phe Gln Leu Phe Ile Gly Trp
130 135 140
Glu Gly Val Gly Ile Met Ser Phe Leu Leu Ile Gly Trp Trp His Gly
145 150 155 160
Arg Thr Asp Ala Asn Thr Ala Ala Leu Gln Ala Ile Leu Tyr Asn Arg
165 170 175
Ile Gly Asp Ile Gly Phe Val Leu Ser Met Ala Trp Phe Leu Thr His
180 185 190
Ser Asn Ala Trp Asp Leu Gln Gln Ile Phe Met Leu Asn Asn Glu Cys
195 200 205
Pro Asn Met Pro Leu Ile Gly Leu Leu Leu Ala Ala Ala Gly Lys Ser
210 215 220
Ala Gln Phe Gly Leu His Pro Trp Leu Pro Ser Ala Met Glu Gly Pro
225 230 235 240
Thr Pro Val Ser Ala Leu Leu His Ser Ser Thr Met Val Val Ala Gly
245 250 255
Val Phe Leu Leu Ile Arg Phe Tyr Pro Leu Met Glu Thr Asn Lys Leu
260 265 270
Val Gln Thr Met Thr Leu Cys Leu Gly Ala Ile Thr Thr Leu Phe Thr
275 280 285
Ala Leu Cys Ala Ile Thr Gln Asn Asp Ile Lys Lys Ile Val Ala Phe
290 295 300
Ser Thr Ser Ser Gln Leu Gly Leu Met Met Val Thr Ile Gly Ile Asn
305 310 315 320
Gln Pro His Leu Ala Phe Leu His Ile Cys Met His Ala Phe Phe Lys
325 330 335
Ala Met Leu Phe Met Cys Ser Gly Ser Ile Ile His Ser Leu Asn Asp
340 345 350
Glu Gln Asp Ile Arg Lys Met Gly Gly Leu Tyr Lys Ala Met Pro Phe
355 360 365
Thr Thr Thr Ala Leu Ile Ile Gly Ser Leu Ala Leu Thr Gly Met Pro
370 375 380
Tyr Leu Thr Gly Phe Tyr Ser Lys Asp Leu Ile Ile Glu Ala Val Asn
385 390 395 400
Met Ser Tyr Thr Asn Ala Trp Ala Leu Leu Met Thr Leu Ile Ala Thr
405 410 415
Ser Leu Thr Ala Ala Tyr Ser Thr Arg Ile Ile Phe Phe Ala Phe Leu
420 425 430
Gly Lys Pro Arg Phe Pro Pro Leu Val Leu Ile Asn Glu Asn Asn Pro
435 440 445
Leu Leu Ile Asn Ser Ile Lys Arg Leu Leu Ile Gly Ser Ile Phe Ala
450 455 460
Gly Phe Ile Ile Ser Asn Asn Ile Pro Pro Met Thr Val Pro Asn Thr
465 470 475 480
Thr Met Pro Leu Tyr Met Lys Met Thr Ala Leu Ile Val Thr Ile Met
485 490 495
Gly Phe Met Leu Ala Leu Glu Leu Asn Asn Thr Thr Tyr Tyr Leu Lys
500 505 510
Phe Lys Tyr Pro Ser Gln Thr Tyr Lys Phe Ser Asn Met Leu Gly Tyr
515 520 525
Tyr Pro Ser Ile Met His Arg Leu Pro Thr Tyr His Asn Leu Ser Met
530 535 540
Ser Gln Lys Ser Ala Ser Ser Leu Leu Asp Leu Ile Trp Leu Glu Thr
545 550 555 560
Ile Leu Pro Lys Thr Thr Ser Phe Ile Gln Met Lys Met Ser Ile Met
565 570 575
Val Ser Asn Gln Lys Gly Leu Ile Lys Leu Tyr Phe Leu Ser Phe Leu
580 585 590
Ile Thr Ile Met Ile Ser Met Thr Leu Phe Asn Tyr His Glu
595 600 605
<210> 20
<211> 175
<212> PRT
<213> Sus scrofa
<400> 20
Met Thr Met Tyr Ile Ala Phe Ile Leu Ser Thr Ile Phe Val Ile Gly
1 5 10 15
Phe Val Gly Phe Ser Ser Lys Pro Ser Pro Ile Tyr Gly Gly Leu Gly
20 25 30
Leu Ile Val Ser Gly Gly Val Gly Cys Gly Ile Val Leu Asn Phe Gly
35 40 45
Gly Ser Phe Leu Gly Leu Met Val Phe Leu Ile Tyr Leu Gly Gly Met
50 55 60
Leu Val Val Phe Gly Tyr Thr Thr Ala Met Ala Thr Glu Met Tyr Pro
65 70 75 80
Glu Val Trp Val Ser Asn Lys Thr Val Phe Gly Ala Phe Val Ser Gly
85 90 95
Leu Met Met Glu Phe Cys Met Val Tyr Tyr Ala Leu Lys Glu Glu Glu
100 105 110
Val Glu Ile Ile Phe Lys Phe Asn Gly Leu Gly Asp Trp Val Ile Tyr
115 120 125
Asp Thr Gly Asp Ser Gly Phe Phe Ser Glu Glu Ala Met Gly Ile Ala
130 135 140
Ala Leu Tyr Ser Tyr Gly Thr Trp Leu Val Ile Val Thr Gly Trp Ser
145 150 155 160
Leu Leu Ile Gly Val Val Val Ile Met Glu Ile Thr Arg Gly Asn
165 170 175
<210> 21
<211> 704
<212> PRT
<213> Sus scrofa
<400> 21
Thr Ala Thr Ala Ala Ser Asn Leu Ile Glu Val Phe Val Asp Gly Gln
1 5 10 15
Ser Val Met Val Glu Pro Gly Thr Thr Val Leu Gln Ala Cys Glu Lys
20 25 30
Val Gly Met Gln Ile Pro Arg Phe Cys Tyr His Glu Arg Leu Ser Val
35 40 45
Ala Gly Asn Cys Arg Met Cys Leu Val Glu Ile Glu Lys Ala Pro Lys
50 55 60
Val Val Ala Ala Cys Ala Met Pro Val Met Lys Gly Trp Asn Ile Leu
65 70 75 80
Thr Asn Ser Glu Lys Ser Lys Lys Ala Arg Glu Gly Val Met Glu Phe
85 90 95
Leu Leu Ala Asn His Pro Leu Asp Cys Pro Ile Cys Asp Gln Gly Gly
100 105 110
Glu Cys Asp Leu Gln Asp Gln Ser Met Met Phe Gly Ser Asp Arg Ser
115 120 125
Arg Phe Leu Glu Gly Lys Arg Ala Val Glu Asp Lys Asn Ile Gly Pro
130 135 140
Leu Val Lys Thr Ile Met Thr Arg Cys Ile Gln Cys Thr Arg Cys Ile
145 150 155 160
Arg Phe Ala Ser Glu Ile Ala Gly Val Asp Asp Leu Gly Thr Thr Gly
165 170 175
Arg Gly Asn Asp Met Gln Val Gly Thr Tyr Ile Glu Lys Met Phe Met
180 185 190
Ser Glu Leu Ser Gly Asn Ile Ile Asp Ile Cys Pro Val Gly Ala Leu
195 200 205
Thr Ser Lys Pro Tyr Ala Phe Thr Ala Arg Pro Trp Glu Thr Arg Lys
210 215 220
Thr Glu Ser Ile Asp Val Met Asp Ala Val Gly Ser Asn Ile Val Val
225 230 235 240
Ser Thr Arg Thr Gly Glu Val Met Arg Ile Leu Pro Arg Met His Glu
245 250 255
Asp Ile Asn Glu Glu Trp Ile Ser Asp Lys Thr Arg Phe Ala Tyr Asp
260 265 270
Gly Leu Lys Arg Gln Arg Leu Thr Gln Pro Met Ile Arg Asn Glu Lys
275 280 285
Gly Leu Leu Thr Tyr Thr Thr Trp Glu Asp Ala Leu Ser Arg Val Ala
290 295 300
Gly Met Leu Gln Ser Phe Gln Gly Asn Asp Val Ala Ala Ile Ala Gly
305 310 315 320
Gly Leu Val Asp Ala Glu Ala Leu Val Ala Leu Lys Asp Leu Leu Asn
325 330 335
Arg Val Asp Ser Asp Ser Leu Cys Thr Glu Glu Val Phe Pro Thr Ala
340 345 350
Gly Ala Gly Thr Asp Leu Arg Ser Asn Tyr Leu Leu Asn Thr Thr Ile
355 360 365
Ala Gly Val Glu Glu Ala Asp Val Ile Leu Leu Val Gly Thr Asn Pro
370 375 380
Arg Phe Glu Ala Pro Leu Phe Asn Ala Arg Ile Arg Lys Ser Trp Leu
385 390 395 400
His Asn Asp Leu Lys Val Ala Leu Ile Gly Ser Pro Val Asp Leu Thr
405 410 415
Tyr Arg Tyr Asp His Leu Gly Asp Ser Pro Lys Ile Leu Gln Asp Ile
420 425 430
Ala Ser Gly Asn His Pro Phe Ser Gln Ile Leu Lys Glu Ala Lys Lys
435 440 445
Pro Met Val Val Leu Gly Ser Ser Ala Leu Gln Arg Ser Asp Gly Thr
450 455 460
Ala Ile Leu Ala Ala Val Ser Asn Ile Ala Gln Asn Ile Arg Leu Ser
465 470 475 480
Ser Gly Val Thr Gly Asp Trp Lys Val Met Asn Ile Leu His Arg Ile
485 490 495
Ala Ser Gln Val Ala Ala Leu Asp Leu Gly Tyr Lys Pro Gly Val Glu
500 505 510
Ala Ile Arg Lys Asn Pro Pro Lys Val Leu Phe Leu Leu Gly Ala Asp
515 520 525
Gly Gly Cys Ile Thr Arg Gln Asp Leu Pro Lys Asp Cys Phe Ile Ile
530 535 540
Tyr Gln Gly His His Gly Asp Val Gly Ala Pro Met Ala Asp Val Ile
545 550 555 560
Leu Pro Gly Ala Ala Tyr Thr Glu Lys Ser Ala Thr Tyr Val Asn Thr
565 570 575
Glu Gly Arg Ala Gln Gln Thr Lys Val Ala Val Thr Pro Pro Gly Leu
580 585 590
Ala Arg Glu Asp Trp Lys Ile Ile Arg Ala Leu Ser Glu Ile Ala Gly
595 600 605
Met Thr Leu Pro Tyr Asp Thr Leu Asp Gln Val Arg Ser Arg Leu Glu
610 615 620
Glu Val Ser Pro Asn Leu Val Arg Tyr Asp Asp Val Glu Gly Ala Asn
625 630 635 640
Tyr Phe Gln Gln Ala Asn Glu Leu Ser Lys Leu Val Asn Gln Gln Leu
645 650 655
Leu Ala Asp Pro Leu Val Pro Pro Gln Leu Thr Ile Lys Asp Phe Tyr
660 665 670
Met Thr Asp Ser Ile Ser Arg Ala Ser Gln Thr Met Ala Lys Cys Val
675 680 685
Lys Ala Val Thr Glu Gly Ile Gln Ala Val Glu Glu Pro Ser Ile Cys
690 695 700
<210> 22
<211> 105
<212> PRT
<213> Sus scrofa
<400> 22
Pro Phe Phe Asp Val Gln Lys Arg Leu Gly Leu Asp Leu Asp Arg Trp
1 5 10 15
Met Thr Ile Gln Ser Ala Glu Gln Pro His Lys Ile Pro Gly Arg Cys
20 25 30
His Ala Phe Glu Lys Glu Trp Ile Glu Cys Ala His Gly Ile Gly Gly
35 40 45
Ile Arg Ala Glu Lys Glu Cys Lys Ile Glu Phe Asp Asp Phe Val Glu
50 55 60
Cys Leu Leu Arg Gln Lys Thr Met Lys Arg Leu Ser Ala Ile Lys Arg
65 70 75 80
Gln Arg Asp Lys Leu Ile Lys Glu Gly Lys Tyr Thr Pro Pro Pro His
85 90 95
His Leu Gly Lys Glu Asp Pro Arg Pro
100 105
<210> 23
<211> 96
<212> PRT
<213> Sus scrofa
<400> 23
Gly Val Arg Thr Ser Pro Thr Gly Glu Lys Val Thr His Thr Gly Gln
1 5 10 15
Ala Tyr Asp Asp Gly Asp Tyr Arg Arg Val Arg Phe Ser Asp Arg Gln
20 25 30
Lys Glu Val Asn Glu Asn Phe Ala Ile Asp Leu Ile Ala Glu Gln Pro
35 40 45
Val Ser Glu Val Gly Ser Arg Val Ile Ser Cys Asp Gly Gly Gly Gly
50 55 60
Ala Leu Gly His Pro Arg Val Tyr Ile Asn Leu Asp Lys Glu Thr Lys
65 70 75 80
Thr Gly Thr Cys Gly Tyr Cys Gly Leu Gln Phe Arg Gln Pro His His
85 90 95
<210> 24
<211> 444
<212> PRT
<213> Sus scrofa
<400> 24
Ser Gly Asp Thr Thr Ala Pro Lys Lys Thr Ser Phe Gly Ser Leu Lys
1 5 10 15
Asp Glu Asp Arg Ile Phe Thr Asn Leu Tyr Gly Arg His Asp Trp Arg
20 25 30
Leu Lys Gly Ala Gln Ser Arg Gly Asp Trp Tyr Lys Thr Lys Glu Ile
35 40 45
Leu Leu Lys Gly Pro Asp Trp Ile Leu Gly Glu Val Lys Thr Ser Gly
50 55 60
Leu Arg Gly Arg Gly Gly Ala Gly Phe Pro Thr Gly Leu Lys Trp Ser
65 70 75 80
Phe Met Asn Lys Pro Ser Asp Gly Arg Pro Lys Tyr Leu Val Val Asn
85 90 95
Ala Asp Glu Gly Glu Pro Gly Thr Cys Lys Asp Arg Glu Ile Ile Arg
100 105 110
His Asp Pro His Lys Leu Val Glu Gly Cys Leu Val Gly Gly Arg Ala
115 120 125
Met Gly Ala Arg Ala Ala Tyr Ile Tyr Ile Arg Gly Glu Phe Tyr Asn
130 135 140
Glu Ala Ser Asn Leu Gln Val Ala Ile Arg Glu Ala Tyr Glu Ala Gly
145 150 155 160
Leu Ile Gly Lys Asn Ala Cys Gly Ser Gly Tyr Asp Phe Asp Val Phe
165 170 175
Val Val Arg Gly Ala Gly Ala Tyr Ile Cys Gly Glu Glu Thr Ala Leu
180 185 190
Ile Glu Ser Ile Glu Gly Lys Gln Gly Lys Pro Arg Leu Lys Pro Pro
195 200 205
Phe Pro Ala Asp Val Gly Val Phe Gly Cys Pro Thr Thr Val Ala Asn
210 215 220
Val Glu Thr Val Ala Val Ser Pro Thr Ile Cys Arg Arg Gly Gly Ala
225 230 235 240
Trp Phe Ala Ser Phe Gly Arg Glu Arg Asn Ser Gly Thr Lys Leu Phe
245 250 255
Asn Ile Ser Gly His Val Asn His Pro Cys Thr Val Glu Glu Glu Met
260 265 270
Ser Val Pro Leu Lys Glu Leu Ile Glu Lys His Ala Gly Gly Val Ile
275 280 285
Gly Gly Trp Asp Asn Leu Leu Ala Val Ile Pro Gly Gly Ser Ser Thr
290 295 300
Pro Leu Ile Pro Lys Ser Val Cys Glu Thr Val Leu Met Asp Phe Asp
305 310 315 320
Ala Leu Val Gln Ala Gln Thr Gly Leu Gly Thr Ala Ala Val Ile Val
325 330 335
Met Asp Arg Ser Thr Asp Ile Val Lys Ala Ile Ala Arg Leu Ile Glu
340 345 350
Phe Tyr Lys His Glu Ser Cys Gly Gln Cys Thr Pro Cys Arg Glu Gly
355 360 365
Val Asp Trp Met Asn Lys Val Met Ala Arg Phe Val Lys Gly Asp Ala
370 375 380
Arg Pro Ala Glu Ile Asp Ser Leu Trp Glu Ile Ser Lys Gln Ile Glu
385 390 395 400
Gly His Thr Ile Cys Ala Leu Gly Asp Gly Ala Ala Trp Pro Val Gln
405 410 415
Gly Leu Ile Arg His Phe Arg Pro Glu Leu Glu Glu Arg Met Gln Gln
420 425 430
Phe Ala Leu Gln His Gln Ala Arg Gln Ala Ala Ser
435 440
<210> 25
<211> 83
<212> PRT
<213> Sus scrofa
<400> 25
Ala Gly Arg Ile Ala Ser Phe Leu Lys Asn Ala Trp Ala Lys Glu Pro
1 5 10 15
Val Leu Val Ala Ser Phe Ala Ile Gly Gly Leu Ala Ile Ile Leu Pro
20 25 30
Ser Leu Ser Pro Tyr Thr Asn Tyr Ala Ile Arg Ile Asn Arg Ala Thr
35 40 45
Pro Tyr Asn Tyr Pro Val Pro Leu Arg Asp Asp Gly Asn Met Pro Asp
50 55 60
Val Pro Ser His Pro Gln Asp Pro Gln Gly Pro Ser Leu Glu Trp Leu
65 70 75 80
Lys Asn Leu
<210> 26
<211> 115
<212> PRT
<213> Sus scrofa
<400> 26
Ala Gly Leu Leu Lys Lys Thr Thr Gly Leu Val Gly Leu Ala Val Cys
1 5 10 15
Glu Thr Pro His Glu Arg Leu Lys Ile Leu Tyr Thr Lys Ile Leu Asp
20 25 30
Val Leu Gly Gln Ile Pro Lys Asn Ala Ala Tyr Arg Lys Tyr Thr Glu
35 40 45
Gln Ile Thr Asn Glu Lys Leu Gly Met Val Lys Ala Glu Pro Asp Val
50 55 60
Lys Lys Leu Glu Glu Gln Leu Gln Gly Gly Gln Ile Glu Glu Val Ile
65 70 75 80
Leu Gln Ala Glu Asn Glu Leu Ser Leu Ala Arg Lys Met Leu Arg Trp
85 90 95
Lys Pro Trp Glu Pro Leu Val Glu Glu Pro Pro Ala Asn Gln Trp Lys
100 105 110
Trp Pro Ile
115
<210> 27
<211> 112
<212> PRT
<213> Sus scrofa
<400> 27
Ala Ser Ala Thr Arg Val Ile Gln Leu Leu Arg Asn Trp Ala Ser Gly
1 5 10 15
Arg Asp Leu Gln Ala Lys Leu Gln Leu Arg Tyr Gln Glu Ile Ser Lys
20 25 30
Arg Thr Gln Pro Pro Pro Lys Leu Pro Val Gly Pro Ser His Lys Leu
35 40 45
Ser Asn Asn Tyr Tyr Cys Thr Arg Asp Gly Arg Arg Glu Ala Met Pro
50 55 60
Pro Ser Ile Val Met Ser Ser Gln Lys Val Leu Ala Ser Gly Lys Pro
65 70 75 80
Ala Glu Ser Ser Ala Val Ala Glu Thr Glu Lys Lys Ala Val Thr Pro
85 90 95
Ala Pro Pro Ile Lys Arg Trp Glu Leu Ser Lys Asp Gln Pro Tyr Leu
100 105 110
<210> 28
<211> 171
<212> PRT
<213> Sus scrofa
<400> 28
Pro Gly Ile Val Glu Leu Pro Thr Leu Glu Asp Leu Lys Val Gln Glu
1 5 10 15
Val Lys Val Ser Ser Ser Val Leu Lys Ala Ala Ala His His Tyr Gly
20 25 30
Ala Gln Cys Asp Lys Pro Asn Lys Glu Phe Met Leu Cys Arg Trp Glu
35 40 45
Glu Lys Asp Pro Arg Arg Cys Leu Glu Glu Gly Lys Leu Val Asn Gln
50 55 60
Cys Ala Leu Asp Phe Phe Arg Gln Ile Lys Arg His Cys Ala Glu Pro
65 70 75 80
Phe Thr Glu Tyr Trp Thr Cys Ile Asp Tyr Ser Gly Leu Gln Leu Phe
85 90 95
Arg His Cys Arg Lys Gln Gln Ala Lys Phe Asp Glu Cys Val Leu Asp
100 105 110
Lys Leu Gly Trp Val Arg Pro Asp Leu Gly Glu Leu Ser Lys Val Thr
115 120 125
Lys Val Lys Thr Asp Arg Pro Leu Pro Glu Asn Pro Tyr His Ser Arg
130 135 140
Ala Arg Pro Glu Pro Asn Pro Glu Ala Glu Gly Asp Leu Lys Pro Ala
145 150 155 160
Lys His Gly Ser Arg Leu Phe Phe Trp Thr Met
165 170
<210> 29
<211> 342
<212> PRT
<213> Sus scrofa
<400> 29
Leu His His Ala Leu Ile Pro His Gly Lys Gly Gly Arg Ser Ser Val
1 5 10 15
Ser Gly Ile Val Ala Thr Val Phe Gly Ala Thr Gly Phe Leu Gly Arg
20 25 30
Tyr Val Val Asn His Leu Gly Arg Met Gly Ser Gln Val Ile Val Pro
35 40 45
Tyr Arg Cys Glu Pro Tyr Asp Thr Met His Leu Arg Pro Met Gly Asp
50 55 60
Leu Gly Gln Ile Ile Phe Met Glu Trp Asn Gly Lys Asp Lys Asp Ser
65 70 75 80
Ile Arg Lys Val Val Glu His Ser Asn Val Val Ile Asn Leu Val Gly
85 90 95
Arg Glu Trp Glu Thr Lys Asn Phe Asp Phe Glu Asp Val Phe Val Lys
100 105 110
Ile Pro His Ala Ile Ala Gln Val Ser Lys Glu Ala Gly Val Glu Lys
115 120 125
Leu Ile His Ile Ser His Leu Asn Ala Asp Ile Lys Ser Pro Ser Arg
130 135 140
Tyr Leu Arg Ser Lys Ala Val Gly Glu Lys Glu Val Arg Ala Ala Phe
145 150 155 160
Pro Glu Ala Thr Ile Ile Lys Pro Ser Asp Ile Phe Gly Arg Glu Asp
165 170 175
Arg Phe Leu Asn Tyr Phe Ala Ser Met Arg Trp Phe Gly Gly Val Pro
180 185 190
Leu Ile Ser Leu Gly Lys Glu Thr Val Lys Gln Pro Val Tyr Ile Val
195 200 205
Asp Val Ser Lys Gly Ile Ile Asn Ala Ile Lys Asp Pro Asp Ala Lys
210 215 220
Gly Lys Thr Phe Ala Phe Val Gly Pro Asn Arg Tyr Leu Leu Phe Asp
225 230 235 240
Leu Val Gln Tyr Ile Phe Ala Val Ala Tyr Arg Pro Phe Leu Pro Tyr
245 250 255
Pro Leu Pro His Phe Ala Tyr Arg Trp Val Gly Arg Leu Phe Glu Val
260 265 270
Ser Pro Phe Glu Pro Trp Thr Thr Arg Asp Lys Val Glu Arg Val His
275 280 285
Met Ser Asp Met Thr Leu Pro His Leu Pro Gly Leu Glu Asp Leu Gly
290 295 300
Ile Gln Ala Thr Pro Leu Glu Leu Lys Ala Ile Glu Val Leu Arg Arg
305 310 315 320
His Arg Thr Tyr Arg Trp Leu Thr Ser Glu Met Glu Asp Val Lys Pro
325 330 335
Ala Lys Thr Val Asn Ile
340
<210> 30
<211> 320
<212> PRT
<213> Sus scrofa
<400> 30
Leu Gln Tyr Gly Pro Leu Ala Phe Val Leu Gly Glu Arg Thr Thr Arg
1 5 10 15
Lys Leu Thr Glu Thr Ser Lys Val Ile Thr Val Asp Gly Asn Ile Cys
20 25 30
Ser Gly Lys Gly Arg Leu Ala Arg Glu Ile Ala Glu Lys Leu Gly Leu
35 40 45
Arg His Phe Pro Glu Ala Gly Ile His Tyr Ala Asp Ser Thr Thr Gly
50 55 60
Asp Gly Lys Pro Leu Asp Val Gln Leu Ser Gly Asn Cys Ser Leu Glu
65 70 75 80
Lys Phe Tyr Asp Asp Pro Lys Ser Asn Asp Gly Asn Ser Tyr Arg Leu
85 90 95
Gln Ser Trp Leu Tyr Ala Ser Arg Leu Leu Gln Tyr Ala Asp Ala Leu
100 105 110
Glu His Leu Leu Ser Thr Gly Gln Gly Val Val Leu Glu Arg Ser Ile
115 120 125
Tyr Ser Asp Phe Val Phe Leu Glu Ala Met Tyr Arg Gln Gly Phe Ile
130 135 140
Arg Lys Gln Cys Val Glu His Tyr Asn Glu Val Lys Lys Val Thr Ala
145 150 155 160
Cys Glu Tyr Leu Pro Pro His Val Val Val Tyr Val Asp Val Pro Val
165 170 175
Pro Glu Ile Gln Ser Arg Ile Gln Lys Lys Gly Asn Pro His Glu Met
180 185 190
Lys Ile Thr Ala Ala Tyr Leu Gln Asp Ile Glu Asn Ala Tyr Lys Lys
195 200 205
Thr Phe Leu Pro Glu Met Ser Glu Lys Cys Glu Val Leu Gln Tyr Ser
210 215 220
Ala Arg Glu Ala Glu Asp Ala Glu Lys Val Val Glu Asp Ile Glu Tyr
225 230 235 240
Leu Lys Cys Asp Lys Gly Pro Trp Pro Asp Gln Asp Asp Arg Thr Phe
245 250 255
His Arg Leu Arg Met Leu Val Gln Asn Lys Leu Glu Val Leu Asn Tyr
260 265 270
Thr Thr Ile Pro Val Tyr Leu Pro Glu Ile Thr Ile Gly Ala His Gln
275 280 285
Ser Asp Arg Val Phe Gln Lys Phe Thr Glu Leu Pro Gly Arg Lys Tyr
290 295 300
Ser Pro Gly Tyr Asn Glu Asp Val Gly Asp Lys Trp Ile Trp Leu Lys
305 310 315 320
<210> 31
<211> 140
<212> PRT
<213> Sus scrofa
<400> 31
Ala Lys Thr Leu Leu His Lys Tyr Ser Asp Ile Pro Glu Gly Thr Glu
1 5 10 15
Cys His Arg Lys Ala Tyr Ala Ser Thr Ser Ile Gly Gly Ala Thr Gly
20 25 30
Leu Ile Val Ser Ala Tyr Ser Ile Ala Leu Lys Pro Pro Ala Ser Phe
35 40 45
Leu Glu Gly Val Ala Arg Thr Gly Arg Tyr Thr Phe Thr Ser Ala Ala
50 55 60
Ile Gly Ala Ile Phe Gly Leu Thr Ser Cys Ile Ser Ala Gln Val Arg
65 70 75 80
Glu Lys Pro Asp Asp Pro Leu Asn Tyr Phe Ile Gly Gly Cys Ala Gly
85 90 95
Gly Leu Thr Leu Gly Ala Arg Thr Arg Ser Tyr Gly Ile Gly Ala Ala
100 105 110
Ala Cys Ala Tyr Met Gly Leu Thr Ala Ala Leu Val Lys Met Gly Gln
115 120 125
Leu Glu Gly Trp Gln Val Phe Ala Glu Pro Lys Val
130 135 140
<210> 32
<211> 145
<212> PRT
<213> Sus scrofa
<400> 32
Met Glu Leu Val Gln Val Leu Arg Arg Gly Leu Gln Gln Val Ser Gly
1 5 10 15
His Gly Gly Leu Arg Gly Tyr Leu Arg Val Leu Phe Arg Ala Asn Asp
20 25 30
Val Arg Val Gly Thr Leu Val Gly Glu Asp Lys Tyr Gly Asn Lys Tyr
35 40 45
Tyr Glu Asp Asn Lys Gln Phe Phe Gly Arg His Arg Trp Val Ile Tyr
50 55 60
Thr Thr Glu Met Asn Gly Arg Asp Thr Phe Trp Asp Val Asp Gly Ser
65 70 75 80
Met Val Pro Pro Glu Trp His Arg Trp Leu His Cys Met Thr Asp Asp
85 90 95
Pro Pro Thr Thr Lys Pro Pro Thr Ala Arg Lys Tyr Ile Trp Thr Asn
100 105 110
His Lys Phe Asn Val Ser Gly Thr Pro Gln Gln Tyr Val Pro Tyr Ser
115 120 125
Thr Thr Arg Lys Lys Ile Gln Glu Trp Val Pro Pro Ser Thr Pro Tyr
130 135 140
Lys
145
<210> 33
<211> 143
<212> PRT
<213> Sus scrofa
<400> 33
Ala Ala Ser Lys Val Lys Gln Asp Met Pro Pro Pro Gly Gly Tyr Gly
1 5 10 15
Pro Ile Asp Tyr Lys Arg Asn Leu Pro Arg Arg Gly Leu Ser Gly Tyr
20 25 30
Ser Met Phe Ala Val Gly Ile Gly Thr Leu Leu Phe Gly Tyr Trp Ser
35 40 45
Met Met Lys Trp Asn Arg Glu Arg Arg Arg Leu Gln Ile Glu Asp Phe
50 55 60
Glu Ala Arg Ile Ala Leu Met Pro Leu Phe Gln Ala Glu Lys Asp Arg
65 70 75 80
Arg Val Leu Gln Met Leu Arg Glu Asn Leu Glu Glu Glu Ala Ile Ile
85 90 95
Met Lys Asp Val Pro Asp Trp Lys Val Gly Glu Ser Val Phe His Thr
100 105 110
Thr Arg Trp Val Thr Pro Met Met Gly Glu Leu Tyr Gly Leu Arg Thr
115 120 125
Asn Glu Glu Ile Leu Ser Ala Thr Tyr Gly Phe Ile Trp Tyr Thr
130 135 140
<210> 34
<211> 57
<212> PRT
<213> Sus scrofa
<400> 34
Met Asn Val Leu Gln Ile Val Arg Asp His Trp Val His Ile Leu Val
1 5 10 15
Pro Val Gly Phe Val Phe Gly Cys Tyr Leu Asp Arg Arg Ser Asp Glu
20 25 30
Lys Leu Thr Ala Phe Arg Asn Lys Ser Leu Leu Phe Lys Arg Glu Leu
35 40 45
Arg Pro Asn Glu Glu Val Thr Trp Lys
50 55
<210> 35
<211> 72
<212> PRT
<213> Sus scrofa
<400> 35
Ala Gly Gly Gly Val His Ile Glu Pro Arg Tyr Arg Gln Phe Pro Gln
1 5 10 15
Leu Thr Arg Ser Gln Leu Ile Gln Ala Glu Phe Phe Ser Ala Thr Met
20 25 30
Trp Phe Trp Ile Leu Trp Arg Phe Trp His Asp Ser Asp Ala Val Leu
35 40 45
Gly His Phe Pro Tyr Pro Asp Pro Ser Gln Trp Thr Asp Glu Glu Leu
50 55 60
Gly Ile Leu Pro Asp Asp Glu Asp
65 70
<210> 36
<211> 97
<212> PRT
<213> Sus scrofa
<400> 36
Ala His Gly His Gly His Glu His Gly His Ser Lys Met Glu Leu Pro
1 5 10 15
Asp Tyr Lys Gln Trp Lys Ile Glu Gly Thr Pro Leu Glu Thr Val Gln
20 25 30
Glu Lys Leu Ala Ala Arg Gly Leu Arg Asp Pro Trp Gly Arg Asn Glu
35 40 45
Ala Trp Arg Tyr Ser Gly Gly Phe Ala Asn Asn Val Ser Phe Val Gly
50 55 60
Ala Leu Leu Lys Gly Phe Lys Trp Gly Phe Ala Ala Phe Val Val Ala
65 70 75 80
Val Gly Ala Glu Tyr Tyr Leu Glu Ser Gln Asn Lys Asp Lys Lys His
85 90 95
His
<210> 37
<211> 128
<212> PRT
<213> Sus scrofa
<400> 37
Ser Phe Pro Lys Tyr Lys Pro Ser Arg Leu Ala Thr Leu Pro Ala Thr
1 5 10 15
Leu Asp Pro Ala Glu Tyr Asp Ile Ser Pro Glu Thr Arg Lys Ala Gln
20 25 30
Ala Glu Arg Leu Ala Ile Arg Ser Arg Leu Lys Arg Glu Tyr Leu Leu
35 40 45
Gln Tyr Asn Asp Pro Asn Arg Leu Gly Val Ile Glu Asp Pro Ala Leu
50 55 60
Ile Arg Trp Thr Tyr Ala Arg Ser Ala Asn Ile Tyr Pro Asn Phe Arg
65 70 75 80
Pro Thr Pro Lys Thr Ser Leu Leu Gly Ala Leu Phe Gly Ile Gly Pro
85 90 95
Leu Phe Phe Trp Tyr Tyr Val Phe Lys Thr Asp Arg Asp Lys Lys Glu
100 105 110
Lys Leu Ile Gln Glu Gly Lys Leu Asp Gln Thr Phe Asn Ile Ser Tyr
115 120 125
<210> 38
<211> 143
<212> PRT
<213> Sus scrofa
<400> 38
Ser Gly Asp His Gly Lys Arg Leu Phe Ile Ile Lys Pro Ser Gly Phe
1 5 10 15
Tyr Asp Arg Arg Phe Leu Lys Leu Met Arg Phe Tyr Ile Leu Leu Thr
20 25 30
Gly Ile Pro Val Ala Ile Gly Ile Thr Leu Val Asn Val Phe Ile Gly
35 40 45
Glu Ala Glu Leu Ala Asp Ile Pro Glu Gly Tyr Val Pro Glu His Trp
50 55 60
Glu Tyr Phe Lys His Pro Ile Ser Arg Trp Ile Ala Arg Thr Phe Tyr
65 70 75 80
Asp Gly Pro Glu Lys Asn Tyr Glu Lys Thr Met Ala Ile Leu Gln Ile
85 90 95
Glu Ala Glu Lys Ala Glu Leu Arg Leu Lys Glu Leu Glu Val Arg Arg
100 105 110
Leu Met Arg Ala Arg Gly Asp Gly Pro Trp Tyr Gln Tyr Pro Thr Ile
115 120 125
Asp Lys Ala Leu Ile Asp His Ser Pro Lys Thr Thr Pro Asp Asn
130 135 140
<210> 39
<211> 127
<212> PRT
<213> Sus scrofa
<400> 39
Ser Gly Tyr Thr Pro Asp Glu Lys Leu Arg Leu Gln Gln Leu Arg Glu
1 5 10 15
Leu Arg Arg Arg Trp Leu Lys Asp Gln Glu Leu Ser Pro Arg Glu Pro
20 25 30
Val Leu Pro Pro Arg Arg Val Trp Pro Met Glu Gln Phe Trp Asn Lys
35 40 45
Phe Leu Gln Asp Gly Ala Pro Trp Lys Asn Val Ile Tyr Lys Thr Tyr
50 55 60
Arg His Ser Ile Phe Ala Val Thr His Val Leu Ile Pro Val Trp Ile
65 70 75 80
Ile His Tyr Tyr Leu Lys Tyr His Val Thr Ala Lys Pro Tyr Thr Val
85 90 95
Val Glu Arg Lys Pro Arg Ile Phe Pro Gly Asp Thr Ile Leu Glu Thr
100 105 110
Gly Glu Val Ile Pro Leu Met Lys Glu Phe Pro Asp Gln His His
115 120 125
<210> 40
<211> 136
<212> PRT
<213> Sus scrofa
<400> 40
Gly Ala His Leu Ala Arg Arg Tyr Leu Gly Asp Ala Ser Lys Glu Pro
1 5 10 15
Asp Pro Leu Arg Met Pro Thr Phe Pro Pro Asp Tyr Gly Phe Pro Glu
20 25 30
Arg Lys Glu Arg Glu Met Val Ala Thr Gln Gln Glu Met Asn Asp Ala
35 40 45
Gln Leu Met Leu Gln Gln Arg Asp Tyr Cys Ala His Tyr Leu Ile Gln
50 55 60
Leu Leu Lys Cys Lys Arg Asp Ser Phe Pro Asn Phe Leu Ala Cys Lys
65 70 75 80
His Glu Gln His Asp Trp Asp Tyr Cys Glu His Leu Asp Tyr Val Lys
85 90 95
Arg Met Lys Glu Phe Glu Arg Glu Arg Arg Leu Leu Gln Arg Lys Lys
100 105 110
Arg Arg Glu Gln Arg Glu Ala Glu Met Ala Arg Gly Gln Gly Pro Gly
115 120 125
Glu Val Ala Pro Glu Met Ala Leu
130 135
<210> 41
<211> 178
<212> PRT
<213> Sus scrofa
<400> 41
Ala Phe Ser Ala Pro Ala Ala Tyr Leu Thr His Gln Gln Lys Val Leu
1 5 10 15
Arg Leu Tyr Lys Arg Ala Leu Arg His Leu Glu Ser Trp Cys Val His
20 25 30
Arg Asp Lys Tyr Arg Tyr Phe Ala Cys Leu Met Arg Ala Arg Phe Asp
35 40 45
Glu His Lys Asn Glu Lys Asp Met Val Lys Ala Thr Gln Leu Leu Arg
50 55 60
Gln Ala Glu Glu Glu Phe Trp Tyr Gly Gln His Pro Gln Pro Tyr Ile
65 70 75 80
Phe Pro Glu Ser Pro Gly Gly Thr Ser Tyr Glu Arg Tyr Glu Cys Tyr
85 90 95
Lys Val Pro Glu Trp Cys Leu Asp Asp Trp His Pro Ser Glu Lys Ala
100 105 110
Met Tyr Pro Asp Tyr Phe Ala Lys Arg Glu Gln Trp Lys Arg Leu Arg
115 120 125
Arg Glu Ser Trp Glu Arg Glu Val Lys Gln Leu Gln Glu Glu Thr Pro
130 135 140
Pro Gly Gly Pro Arg Thr Glu Ala Leu Pro Pro Ala Arg Lys Glu Gly
145 150 155 160
Asp Leu Pro Pro Leu Trp Trp His Ile Val Thr Arg Pro Arg Glu Arg
165 170 175
Pro Met
<210> 42
<211> 175
<212> PRT
<213> Sus scrofa
<400> 42
Pro Asp Ser Trp Asp Lys Asp Val Tyr Pro Glu Pro Pro Arg Arg Thr
1 5 10 15
Pro Ala Pro Ala Pro Gln Thr Ser Leu Pro Asn Pro Val Thr Tyr Leu
20 25 30
Thr Lys Ile Phe Asp Leu Leu Val Asp Arg Pro Val Thr Leu Ala Arg
35 40 45
Glu Phe Ile Glu Gln Gln His Ala Lys Asn Arg Tyr Tyr Tyr Tyr His
50 55 60
Arg Glu Phe Arg Arg Val Pro Asp Ile Thr Glu Cys Glu Glu Lys Asp
65 70 75 80
Ile Leu Cys Met Phe Glu Ala Glu Met Gln Trp Arg Arg Asp Tyr Lys
85 90 95
Val Asp Gln Glu Ile Val Asn Ile Ile Gln Glu Arg Leu Lys Ala Cys
100 105 110
Gln Gln Arg Glu Gly Glu Ser Tyr Arg Gln Asn Cys Ala Lys Glu Leu
115 120 125
Glu Gln Phe Thr Gln Val Ser Lys Ala Phe Gln Asp Arg Tyr Ser Asp
130 135 140
Leu Gly Ala His Tyr Ser Ala Arg Lys Cys Leu Ala Lys Gln Lys Gln
145 150 155 160
Arg Met Leu Ala Glu Arg Lys Ala Ala Lys Glu Ala Ala Ala Ala
165 170 175
<210> 43
<211> 125
<212> PRT
<213> Sus scrofa
<400> 43
Glu Ser Gly Ser Ser Arg Ala Val Ile Ala Pro Ser Ala Leu Val Gly
1 5 10 15
Lys Arg Pro Pro Glu Pro Thr Ile Arg Leu Gln Glu Asp Pro Asp Pro
20 25 30
Glu Asp Glu Asn Leu Tyr Glu Lys Asn Pro Asp Ser His Gly Tyr Asp
35 40 45
Lys Asp Pro Ile Val Asp Leu Trp Asn Met Arg Val Val Phe Phe Phe
50 55 60
Gly Phe Ser Ile Val Leu Val Leu Gly Ser Thr Phe Val Ala Tyr Leu
65 70 75 80
Pro Asp Tyr Arg Met Gln Glu Trp Ala Arg Arg Glu Ala Glu Arg Leu
85 90 95
Val Lys Tyr Arg Glu Ala Asn Gly Leu Pro Leu Met Glu Ser Asn Cys
100 105 110
Phe Asp Pro Asn Lys Ile Gln Leu Pro Glu Asp Glu Asp
115 120 125
<210> 44
<211> 122
<212> PRT
<213> Sus scrofa
<400> 44
Met Thr Met Met Ser Gly Arg Pro Gly Arg Val Pro Leu Gln Phe Leu
1 5 10 15
Pro Asn Glu Ala Arg Ser Leu Pro Pro Pro Lys Leu Thr Asp Pro Arg
20 25 30
Leu Val Tyr Met Gly Phe Leu Gly Tyr Cys Ser Gly Leu Ile Asp Asn
35 40 45
Ala Ile Arg Arg Arg Pro Val Val Ser Ala Gly Leu His Arg Gln Leu
50 55 60
Leu Tyr Val Thr Ser Phe Val Phe Phe Gly Tyr Tyr Leu Leu Lys Arg
65 70 75 80
Gln Asp Tyr Met Tyr Ala Leu Arg Asp His Asp Met Phe Ala Tyr Val
85 90 95
Lys Ser His Pro Glu Asp Phe Pro Glu Lys Asp Lys Lys Thr Tyr Gly
100 105 110
Glu Ile Leu Glu Glu Phe His Pro Val Arg
115 120
<210> 45
<211> 471
<212> PRT
<213> Sus scrofa
<400> 45
Met Ala Ala Ser Leu Leu Leu Arg Gln Gly Arg Ala Arg Ala Leu Lys
1 5 10 15
Thr Val Leu Leu Glu Gly Gly Val Phe Arg Gly Leu Ala Pro Ala Val
20 25 30
Ser Leu Ser Ala Glu Ser Gly Arg Asn Glu Lys Glu Arg Pro Pro Asn
35 40 45
Pro Lys Lys Gln Ser Pro Pro Lys Asn Val Val Ala Ala Gln Glu Gly
50 55 60
Gly Thr Pro Leu Ala Thr Pro Ala Ala Pro Glu Leu Ser Gln Gln Leu
65 70 75 80
Ser Ser Pro Thr Ser Pro Pro Ala Ala Ala Arg Pro Gly Gly Thr Leu
85 90 95
Ala Pro Pro Asn Pro Gly Asp Ser Thr Leu Phe Thr Asp Arg Gly Arg
100 105 110
Pro Arg Phe Pro Ser Arg Lys Thr Leu Val Glu Phe Pro Arg Lys Val
115 120 125
Leu Ser Pro Phe Ser Gln Gln Gly Ser Asp Ser Ala Ala Thr Gln Ala
130 135 140
Ser Arg Gly Arg Ala Gly Asp Ala Thr Ser Ser Ser Ser Ser Ser Ser
145 150 155 160
Ser Ser Asp Ser Glu Ser Asp Asp Glu Glu Glu Gly Asp Gly Ser Glu
165 170 175
Ala Val Arg Pro Val Lys Ser Thr Ser Pro Gly Gly Ser Pro Lys Ala
180 185 190
Glu Ala Pro Arg Ser Leu Ala Gly Gly Ala Pro Gln Thr Gly Val Pro
195 200 205
Ala Glu Ala Arg Pro Gly Pro Gln Gln Pro His Pro Asp Leu Ala Ala
210 215 220
Ala Glu Arg Pro Arg Gln Ala Lys Gly Lys Gly Gly Ser Gly Ala Pro
225 230 235 240
Glu Asp Arg Arg Ala Pro Lys Ala Ala Ala Pro Gly Ser Arg Gly Gly
245 250 255
Pro Gly Ala Ala Arg Gln Ser Glu Thr Pro Ser Gln Lys Ile Pro Arg
260 265 270
Ser Glu Glu Ala Asp Ala Glu Ser Gln Lys Pro Pro Glu Val Lys Thr
275 280 285
Ser Leu Pro Asp Pro Thr Lys Ser Gly Leu Ser Thr Ala Pro Arg Gly
290 295 300
Gly Pro Ala Ser Ala Glu Pro Ala Ala Thr Pro Ala Gly Ala Arg Leu
305 310 315 320
Pro Ala Thr Pro Leu Glu Thr Gly Glu Arg Pro Leu Gly Glu Arg Val
325 330 335
Pro Glu Pro Gly Gly Gln Val Ala Ser Pro Leu Phe Ser Arg Ala Pro
340 345 350
Leu Gly Gly Gln Ala Ala Glu Gly Val Ala Glu Ala Lys Gly Glu Leu
355 360 365
Leu Glu Gly Arg Pro Leu Val Gln Gly Pro Lys Ala Val Pro Asp Gly
370 375 380
Gln Asp Glu Gly Ala Glu Lys Lys Ala Leu Arg Pro Glu Glu Glu Ala
385 390 395 400
Gly Ile Ala Gly Asp Ala Ala Pro Gly Thr Ala Gly Arg Asp Ala Thr
405 410 415
Gln Glu Pro Thr Pro Ala Ala Ala Ala Ala Glu Pro Phe Asp Asn Ser
420 425 430
Thr Tyr Arg Asn Leu Gln His His Glu Tyr Ser Thr Tyr Thr Phe Leu
435 440 445
Asp Leu Asn Val Glu Leu Ser Lys Phe Arg Met Pro Gln Pro Ser Ser
450 455 460
Gly Arg Gln Ser Pro Arg His
465 470
<210> 46
<211> 196
<212> PRT
<213> Sus scrofa
<400> 46
Ser His Thr Asp Ile Arg Val Pro Asp Phe Ser Asp Tyr Arg Arg Ala
1 5 10 15
Glu Val Leu Asp Ser Thr Lys Ser Ser Lys Glu Ser Ser Asp Ala Arg
20 25 30
Lys Gly Phe Ser Tyr Leu Ile Thr Ala Thr Thr Thr Val Gly Val Ala
35 40 45
Tyr Ala Ala Lys Asn Ala Val Ser Gln Phe Val Ser Ser Met Ser Ala
50 55 60
Ser Ala Asp Val Leu Ala Met Ser Lys Ile Glu Ile Lys Leu Ser Asp
65 70 75 80
Ile Pro Glu Gly Lys Asn Met Ala Phe Lys Trp Arg Gly Lys Pro Leu
85 90 95
Phe Val Arg His Arg Thr Lys Lys Glu Ile Asp Gln Glu Ala Ala Val
100 105 110
Glu Val Ser Gln Leu Arg Asp Pro Gln His Asp Leu Glu Arg Val Lys
115 120 125
Lys Pro Glu Trp Val Ile Leu Ile Gly Val Cys Thr His Leu Gly Cys
130 135 140
Val Pro Ile Ala Asn Ala Gly Asp Phe Gly Gly Tyr Tyr Cys Pro Cys
145 150 155 160
His Gly Ser His Tyr Asp Ala Ser Gly Arg Ile Arg Lys Gly Pro Ala
165 170 175
Pro Leu Asn Leu Glu Val Pro Thr Tyr Glu Phe Thr Ser Asp Asp Leu
180 185 190
Val Ile Val Gly
195
<210> 47
<211> 379
<212> PRT
<213> Sus scrofa
<400> 47
Met Thr Asn Ile Arg Lys Ser His Pro Leu Met Lys Ile Ile Asn Asn
1 5 10 15
Ala Phe Ile Asp Leu Pro Ala Pro Ser Asn Ile Ser Ser Trp Trp Asn
20 25 30
Phe Gly Ser Leu Leu Gly Ile Cys Leu Ile Leu Gln Ile Leu Thr Gly
35 40 45
Leu Phe Leu Ala Met His Tyr Thr Ser Asp Thr Thr Thr Ala Phe Ser
50 55 60
Ser Val Thr His Ile Cys Arg Asp Val Asn Tyr Gly Trp Val Ile Arg
65 70 75 80
Tyr Leu His Ala Asn Gly Ala Ser Met Phe Phe Ile Cys Leu Phe Ile
85 90 95
His Val Gly Arg Gly Leu Tyr Tyr Gly Ser Tyr Met Phe Leu Glu Thr
100 105 110
Trp Asn Ile Gly Val Val Leu Leu Phe Thr Val Met Ala Thr Ala Phe
115 120 125
Met Gly Tyr Val Leu Pro Trp Gly Gln Met Ser Phe Trp Gly Ala Thr
130 135 140
Val Ile Thr Asn Leu Leu Ser Ala Ile Pro Tyr Ile Gly Thr Asp Leu
145 150 155 160
Val Glu Trp Ile Trp Gly Gly Phe Ser Val Asp Lys Ala Thr Leu Thr
165 170 175
Arg Phe Phe Ala Phe His Phe Ile Leu Pro Phe Ile Ile Thr Ala Leu
180 185 190
Ala Ala Val His Leu Leu Phe Leu His Glu Thr Gly Ser Asn Asn Pro
195 200 205
Thr Gly Ile Ser Ser Asp Met Asp Lys Ile Pro Phe His Pro Tyr Tyr
210 215 220
Thr Ile Lys Asp Ile Leu Gly Ala Leu Phe Met Met Leu Ile Leu Leu
225 230 235 240
Ile Leu Val Leu Phe Ser Pro Asp Leu Leu Gly Asp Pro Asp Asn Tyr
245 250 255
Thr Pro Ala Asn Pro Leu Asn Thr Pro Pro His Ile Lys Pro Glu Trp
260 265 270
Tyr Phe Leu Phe Ala Tyr Ala Ile Leu Arg Ser Ile Pro Asn Lys Leu
275 280 285
Gly Gly Val Leu Ala Leu Val Ala Ser Ile Leu Ile Leu Ile Leu Met
290 295 300
Pro Met Leu His Thr Ser Lys Gln Arg Ser Met Met Phe Arg Pro Leu
305 310 315 320
Ser Gln Cys Leu Phe Trp Met Leu Val Ala Asp Leu Ile Thr Leu Thr
325 330 335
Trp Ile Gly Gly Gln Pro Val Glu His Pro Phe Ile Ile Ile Gly Gln
340 345 350
Leu Ala Ser Ile Leu Tyr Phe Leu Ile Ile Leu Val Leu Met Pro Ile
355 360 365
Thr Ser Ile Ile Glu Asn Asn Leu Leu Lys Trp
370 375
<210> 48
<211> 326
<212> PRT
<213> Sus scrofa
<400> 48
Met Ser Ala Ala Ala Ala Ala Ser Leu Arg Gly Ala Val Leu Gly Pro
1 5 10 15
Arg Gly Ala Gly Leu Pro Gly Ala Arg Ala Arg Gly Leu Leu Cys Gly
20 25 30
Pro Arg Pro Gly Gln Leu Pro Leu Arg Thr Pro Gln Ala Leu Ser Leu
35 40 45
Ser Ser Lys Ser Gly Leu Ser Arg Gly Arg Lys Val Ile Leu Ser Ala
50 55 60
Leu Gly Met Leu Ala Ala Gly Gly Ala Gly Leu Ala Val Ala Leu His
65 70 75 80
Ser Ala Val Ser Ala Thr Asp Leu Glu Leu His Ala Pro Ser Tyr Pro
85 90 95
Trp Ser His Arg Gly Leu Leu Ser Ser Leu Asp His Thr Ser Ile Arg
100 105 110
Arg Gly Phe Gln Val Tyr Lys Gln Val Cys Ser Ser Cys His Ser Met
115 120 125
Asp Tyr Val Ala Tyr Arg His Leu Val Gly Val Cys Tyr Thr Glu Glu
130 135 140
Glu Ala Lys Ala Leu Ala Glu Glu Val Glu Val Gln Asp Gly Pro Asn
145 150 155 160
Glu Asp Gly Glu Met Phe Met Arg Pro Gly Lys Leu Ser Asp Tyr Phe
165 170 175
Pro Lys Pro Tyr Pro Asn Pro Glu Ala Ala Arg Ala Ala Asn Asn Gly
180 185 190
Ala Leu Pro Pro Asp Leu Ser Tyr Ile Val Arg Ala Arg His Gly Gly
195 200 205
Glu Asp Tyr Val Phe Ser Leu Leu Thr Gly Tyr Cys Glu Pro Pro Thr
210 215 220
Gly Val Ser Leu Arg Glu Gly Leu Tyr Phe Asn Pro Tyr Phe Pro Gly
225 230 235 240
Gln Ala Ile Ala Met Ala Pro Pro Ile Tyr Asn Glu Val Leu Glu Phe
245 250 255
Asp Asp Gly Thr Pro Ala Thr Met Ser Gln Val Ala Lys Asp Val Cys
260 265 270
Thr Phe Leu Arg Trp Ala Ser Glu Pro Glu His Asp His Arg Lys Arg
275 280 285
Met Gly Leu Lys Met Leu Met Met Met Gly Leu Leu Leu Pro Leu Val
290 295 300
Tyr Ala Met Lys Arg His Lys Trp Ser Val Leu Lys Ser Arg Lys Leu
305 310 315 320
Ala Tyr Arg Pro Pro Lys
325
<210> 49
<211> 480
<212> PRT
<213> Sus scrofa
<400> 49
Met Ala Ala Ser Ala Val Cys Arg Val Ala Gly Thr Gly Ser Arg Val
1 5 10 15
Leu Leu Arg Thr Cys Arg Ser Pro Ala Leu Leu Arg Ser Pro Ala Leu
20 25 30
Arg Gly Thr Ala Thr Tyr Ala Gln Ala Leu Gln Ser Val Pro Glu Thr
35 40 45
Gln Val Ser Gln Leu Asp Asn Gly Leu Arg Val Ala Ser Glu Gln Ser
50 55 60
Ser Gln Pro Thr Cys Thr Val Gly Val Trp Ile Asp Ala Gly Ser Arg
65 70 75 80
Tyr Glu Asn Glu Lys Asn Asn Gly Ala Gly Tyr Phe Val Glu His Leu
85 90 95
Ala Phe Lys Gly Thr Lys Asn Arg Pro Gly Ser Ala Leu Glu Lys Glu
100 105 110
Val Glu Ser Met Gly Ala His Leu Asn Ala Tyr Ser Thr Arg Glu His
115 120 125
Thr Ala Tyr Tyr Ile Lys Ala Leu Ser Lys Asp Leu Pro Lys Ala Val
130 135 140
Glu Leu Leu Ala Asp Ile Val Gln Asn Cys Ser Leu Glu Asp Ser Gln
145 150 155 160
Ile Glu Lys Glu Arg Asp Val Ile Leu Gln Glu Leu Gln Glu Asn Asp
165 170 175
Ser Ser Met Arg Asp Val Val Phe Asp Tyr Leu His Ala Thr Ala Phe
180 185 190
Gln Gly Thr Pro Leu Ala Gln Ser Val Glu Gly Pro Ser Glu Asn Val
195 200 205
Arg Lys Leu Ser Arg Ala Asp Leu Thr Glu Tyr Val Ser Gln His Tyr
210 215 220
Lys Ala Pro Arg Met Val Leu Ala Ala Ala Gly Gly Val Glu His Arg
225 230 235 240
Gln Leu Leu Asp Leu Ala Gln Lys His Phe Ser Ser Leu Ser Gly Thr
245 250 255
Tyr Val Glu Asp Ala Val Pro Ala Phe Thr Pro Cys Arg Phe Thr Gly
260 265 270
Ser Glu Ile Arg His Arg Asp Asp Ala Leu Pro Leu Ala His Val Ala
275 280 285
Ile Ala Val Glu Gly Pro Gly Trp Ala Asn Pro Asp Asn Val Pro Leu
290 295 300
Gln Val Ala Asn Ala Ile Ile Gly His Tyr Asp Ser Thr Tyr Gly Gly
305 310 315 320
Gly Thr His Met Ser Ser Thr Leu Ala Ser Val Ala Ala Thr Arg Lys
325 330 335
Leu Cys Gln Ser Phe Gln Thr Phe Asn Ile Cys Tyr Ala Glu Thr Gly
340 345 350
Leu Leu Gly Ala His Phe Val Cys Asp Asn Met Ser Ile Asp Asp Met
355 360 365
Met Phe Phe Leu Gln Gly Gln Trp Met Arg Leu Cys Thr Ser Ala Thr
370 375 380
Glu Ser Glu Val Val Arg Gly Lys Asn Ile Leu Arg Asn Ala Leu Val
385 390 395 400
Ser His Leu Asp Gly Thr Thr Pro Val Cys Glu Asp Ile Gly Arg Ser
405 410 415
Leu Leu Thr Tyr Gly Arg Arg Ile Pro Leu Ala Glu Trp Glu Ser Arg
420 425 430
Ile Ala Glu Val Asp Ala Ser Val Val Arg Glu Val Cys Ser Lys Tyr
435 440 445
Phe Tyr Asp Gln Cys Pro Ala Val Ala Gly Leu Gly Pro Ile Glu Gln
450 455 460
Leu Pro Asp Tyr Asn Arg Ile Arg Ser Gly Met Phe Trp Leu Arg Phe
465 470 475 480
<210> 50
<211> 453
<212> PRT
<213> Sus scrofa
<400> 50
Met Lys Leu Ile Thr Arg Ala Gly Ser Phe Ser Arg Phe Tyr Ser Leu
1 5 10 15
Lys Val Ala Pro Lys Ala Leu Ala Ser Ala Ala Pro Ala Gly Val Pro
20 25 30
Leu Gln Pro Gln Asp Leu Glu Phe Thr Arg Leu Pro Asn Gly Leu Val
35 40 45
Ile Ala Ser Leu Glu Asn Tyr Ala Pro Ala Ser Arg Ile Gly Leu Phe
50 55 60
Ile Lys Ala Gly Ser Arg Tyr Glu Asp Ser Asn Asn Leu Gly Thr Ser
65 70 75 80
His Leu Leu Arg Leu Ala Ser Ser Leu Thr Thr Lys Gly Ala Ser Ser
85 90 95
Phe Lys Ile Thr Arg Gly Ile Glu Ala Val Gly Gly Lys Leu Ser Val
100 105 110
Thr Ser Thr Arg Glu Ser Met Ala Tyr Thr Val Glu Cys Leu Arg Asp
115 120 125
Asp Ile Glu Ile Leu Met Glu Phe Leu Leu Asn Val Thr Ala Ala Pro
130 135 140
Glu Phe Arg Arg Trp Glu Val Ala Ala Leu Gln Ser Gln Leu Arg Ile
145 150 155 160
Asp Lys Ala Val Ala Phe Gln Asn Pro Gln Ala Gln Val Leu Glu Asn
165 170 175
Leu His Ala Ala Ala Tyr Arg Asn Ala Leu Ala Asn Ser Leu Tyr Cys
180 185 190
Pro Asp Tyr Arg Ile Gly Lys Val Thr Pro Asp Gln Leu His Tyr Tyr
195 200 205
Val Gln Asn His Phe Thr Ser Ala Arg Met Ala Leu Ile Gly Leu Gly
210 215 220
Val Ser His Pro Val Leu Lys Gln Val Ala Glu Arg Phe Leu Asn Met
225 230 235 240
Arg Gly Gly Leu Gly Leu Ser Gly Ala Lys Ala Lys Tyr Arg Gly Gly
245 250 255
Glu Ile Arg Asp Gln Asn Gly Asp Ser Leu Val His Ala Ala Leu Val
260 265 270
Ala Glu Ser Ala Ala Thr Gly Ser Ala Glu Ala Asn Ala Phe Ser Val
275 280 285
Leu Gln His Val Leu Gly Ala Gly Pro His Val Lys Arg Gly Ser Asn
290 295 300
Ala Thr Ser Ser Leu Tyr Gln Ala Val Ala Lys Gly Val His Gln Pro
305 310 315 320
Phe Asp Val Ser Ala Phe Asn Ala Ser Tyr Ser Asp Ser Gly Leu Phe
325 330 335
Gly Ile Tyr Thr Ile Ser Gln Ala Ala Ser Ala Gly Asp Val Ile Lys
340 345 350
Ser Ala Tyr Asp Gln Val Lys Ala Ile Ala Gln Gly Asn Leu Ser Asn
355 360 365
Thr Asp Val Gln Ala Ala Lys Asn Lys Leu Lys Ala Gly Tyr Leu Met
370 375 380
Ser Val Glu Ser Ser Glu Gly Phe Leu Asp Glu Val Gly Ser Gln Ala
385 390 395 400
Leu Val Ala Gly Ser Tyr Met Gln Pro Ser Thr Val Leu Gln Gln Ile
405 410 415
Asp Ser Val Ala Asp Ala Asp Val Ile Asn Ala Ala Lys Lys Phe Val
420 425 430
Ser Gly Arg Lys Ser Met Ala Ala Ser Gly Asn Leu Gly His Thr Pro
435 440 445
Phe Val Asp Glu Leu
450
<210> 51
<211> 91
<212> PRT
<213> Sus scrofa
<400> 51
Met Gly Leu Glu Asp Glu Gln Arg Met Leu Thr Gly Ser Gly Asp Pro
1 5 10 15
Lys Glu Glu Glu Glu Glu Glu Glu Glu Leu Val Asp Pro Leu Thr Thr
20 25 30
Val Arg Glu Gln Cys Glu Gln Ile Glu Lys Cys Ile Lys Ala Arg Glu
35 40 45
Arg Leu Glu Leu Cys Asp Gln Arg Val Ser Ser Arg Ser Gln Thr Glu
50 55 60
Glu Asp Cys Thr Glu Glu Leu Phe Asp Phe Leu His Ala Arg Asp His
65 70 75 80
Cys Val Ala His Lys Leu Phe Asn Ser Leu Lys
85 90
<210> 52
<211> 111
<212> PRT
<213> Sus scrofa
<400> 52
Met Ala Ser Arg Pro Ala Val Ala Ala Ser Ser Lys Trp Leu Glu Gly
1 5 10 15
Ile Arg Lys Trp Tyr Tyr Asn Ala Ala Gly Phe Asn Lys Leu Gly Leu
20 25 30
Met Arg Asp Asp Thr Ile Tyr Glu Asp Asp Asp Val Lys Glu Ala Ile
35 40 45
Arg Arg Leu Pro Glu Asn Leu Tyr Asn Asp Arg Val Phe Arg Ile Lys
50 55 60
Arg Ala Leu Asp Leu Thr Met Arg Gln Gln Ile Leu Pro Lys Glu Gln
65 70 75 80
Trp Thr Lys Tyr Glu Glu Asp Lys Phe Tyr Leu Glu Pro Tyr Leu Lys
85 90 95
Glu Val Ile Arg Glu Arg Lys Glu Arg Glu Glu Trp Ala Lys Lys
100 105 110
<210> 53
<211> 82
<212> PRT
<213> Sus scrofa
<400> 53
Met Gly Arg Glu Phe Gly His Leu Thr Arg Met Arg His Val Ile Thr
1 5 10 15
Tyr Ser Leu Ser Pro Phe Glu Gln Arg Ala Phe Pro His Tyr Phe Thr
20 25 30
Lys Gly Ile Pro Asn Val Leu Arg Arg Thr Arg Ala Cys Ile Leu Arg
35 40 45
Val Ala Pro Pro Phe Val Val Phe Tyr Leu Val Tyr Thr Trp Gly Thr
50 55 60
Gln Glu Phe Glu Lys Ser Lys Arg Lys Asn Pro Ala Ala Tyr Glu Asn
65 70 75 80
Asp Lys
<210> 54
<211> 64
<212> PRT
<213> Sus scrofa
<400> 54
Met Ala Ala Pro Thr Leu Thr Ala Arg Leu Tyr Ser Leu Leu Phe Arg
1 5 10 15
Arg Thr Ser Thr Phe Ala Leu Thr Ile Ala Val Gly Ala Leu Phe Phe
20 25 30
Glu Arg Ala Phe Asp Gln Gly Ala Asp Ala Ile Tyr Glu His Ile Asn
35 40 45
Gln Gly Lys Leu Trp Lys His Ile Lys His Lys Tyr Glu Asn Lys Glu
50 55 60
<210> 55
<211> 56
<212> PRT
<213> Sus scrofa
<400> 55
Met Leu Ser Arg Phe Leu Gly Pro Arg Tyr Arg Glu Leu Ala Arg Asn
1 5 10 15
Trp Ile Pro Thr Ala Ser Met Trp Gly Ala Val Gly Ala Val Gly Leu
20 25 30
Val Trp Ala Thr Asp Trp Arg Leu Ile Leu Asp Trp Val Pro Tyr Ile
35 40 45
Asn Gly Lys Phe Lys Lys Glu Asp
50 55
<210> 56
<211> 78
<212> PRT
<213> Sus scrofa
<400> 56
Met Leu Ser Val Ala Ser Arg Ser Gly Pro Phe Ala Pro Val Leu Ser
1 5 10 15
Ala Thr Ser Arg Gly Val Ala Gly Ala Leu Arg Pro Leu Val Gln Ala
20 25 30
Ala Leu Pro Ala Thr Ser Glu Ser Pro Val Leu Asp Ala Lys Arg Ser
35 40 45
Phe Leu Cys Arg Glu Ser Leu Ser Gly Gln Ala Ala Gly Arg Pro Leu
50 55 60
Val Ala Ser Val Gly Leu Asn Val Pro Ala Ser Val Arg Tyr
65 70 75
<210> 57
<211> 261
<212> PRT
<213> Sus scrofa
<400> 57
Met Thr His Gln Thr His Ala Tyr His Met Val Asn Pro Ser Pro Trp
1 5 10 15
Pro Leu Thr Gly Ala Leu Ser Ala Leu Leu Met Thr Ser Gly Leu Thr
20 25 30
Met Trp Phe His Phe Asn Ser Met Leu Leu Leu Ser Leu Gly Leu Leu
35 40 45
Thr Asn Thr Leu Thr Met Tyr Gln Trp Trp Arg Asp Ile Ile Arg Glu
50 55 60
Ser Thr Phe Gln Gly His His Thr Ser Val Val Gln Lys Gly Leu Arg
65 70 75 80
Tyr Gly Met Ile Leu Phe Ile Ile Ser Glu Val Leu Phe Phe Thr Gly
85 90 95
Phe Phe Trp Ala Phe Tyr His Ser Ser Leu Ala Pro Thr Pro Glu Leu
100 105 110
Gly Gly Cys Trp Pro Pro Thr Gly Ile His Pro Leu Asn Pro Leu Glu
115 120 125
Val Pro Leu Leu Asn Thr Ser Ile Leu Leu Ala Ser Gly Val Ser Ile
130 135 140
Thr Trp Ala His His Ser Leu Met Glu Gly Asp Arg Lys His Met Ile
145 150 155 160
Gln Ala Leu Ser Ile Thr Ile Ala Leu Gly Val Tyr Phe Thr Leu Leu
165 170 175
Gln Ala Ser Glu Tyr Tyr Glu Ala Pro Phe Thr Ile Ser Asp Gly Val
180 185 190
Tyr Gly Ser Thr Phe Phe Val Ala Thr Gly Phe His Gly Leu His Val
195 200 205
Ile Ile Gly Ser Thr Phe Leu Ala Val Cys Leu Leu Arg Gln Leu Lys
210 215 220
Phe His Phe Thr Ser Asn His His Phe Gly Phe Glu Ala Ala Ala Trp
225 230 235 240
Tyr Trp His Phe Val Asp Val Val Trp Leu Phe Leu Tyr Val Ser Ile
245 250 255
Tyr Trp Trp Gly Ser
260
<210> 58
<211> 514
<212> PRT
<213> Sus scrofa
<400> 58
Met Phe Val Asn Arg Trp Leu Tyr Ser Thr Asn His Lys Asp Ile Gly
1 5 10 15
Thr Leu Tyr Leu Leu Phe Gly Ala Trp Ala Gly Met Val Gly Thr Ala
20 25 30
Leu Ser Leu Leu Ile Arg Ala Glu Leu Gly Gln Pro Gly Thr Leu Leu
35 40 45
Gly Asp Asp Gln Ile Tyr Asn Val Ile Val Thr Ala His Ala Phe Val
50 55 60
Met Ile Phe Phe Met Val Met Pro Ile Met Ile Gly Gly Phe Gly Asn
65 70 75 80
Trp Leu Val Pro Leu Met Ile Gly Ala Pro Asp Met Ala Phe Pro Arg
85 90 95
Met Asn Asn Met Ser Phe Trp Leu Leu Pro Pro Ser Phe Leu Leu Leu
100 105 110
Leu Ala Ser Ser Met Val Glu Ala Gly Ala Gly Thr Gly Trp Thr Val
115 120 125
Tyr Pro Pro Leu Ala Gly Asn Leu Ala His Ala Gly Ala Ser Val Asp
130 135 140
Leu Thr Ile Phe Ser Leu His Leu Ala Gly Val Ser Ser Ile Leu Gly
145 150 155 160
Ala Ile Asn Phe Ile Thr Thr Ile Ile Asn Met Lys Pro Pro Ala Met
165 170 175
Ser Gln Tyr Gln Thr Pro Leu Phe Val Trp Ser Val Leu Ile Thr Ala
180 185 190
Val Leu Leu Leu Leu Ser Leu Pro Val Leu Ala Ala Gly Ile Thr Met
195 200 205
Leu Leu Thr Asp Arg Asn Leu Asn Thr Thr Phe Phe Asp Pro Ala Gly
210 215 220
Gly Gly Asp Pro Ile Leu Tyr Gln His Leu Phe Trp Phe Phe Gly His
225 230 235 240
Pro Glu Val Tyr Ile Leu Ile Leu Pro Gly Phe Gly Met Ile Ser His
245 250 255
Ile Val Thr Tyr Tyr Ser Gly Lys Lys Glu Pro Phe Gly Tyr Met Gly
260 265 270
Met Val Trp Ala Met Met Ser Ile Gly Phe Leu Gly Phe Ile Val Trp
275 280 285
Ala His His Met Phe Thr Val Gly Met Asp Val Asp Thr Arg Ala Tyr
290 295 300
Phe Thr Ser Ala Thr Met Ile Ile Ala Ile Pro Thr Gly Val Lys Val
305 310 315 320
Phe Ser Trp Leu Ala Thr Leu His Gly Gly Asn Ile Lys Trp Ser Pro
325 330 335
Ala Met Leu Trp Ala Leu Gly Phe Ile Phe Leu Phe Thr Val Gly Gly
340 345 350
Leu Thr Gly Ile Val Leu Ala Asn Ser Ser Leu Asp Ile Val Leu His
355 360 365
Asp Thr Tyr Tyr Val Val Ala His Phe His Tyr Val Leu Ser Met Gly
370 375 380
Ala Val Phe Ala Ile Met Gly Gly Phe Val His Trp Phe Pro Leu Phe
385 390 395 400
Ser Gly Tyr Thr Leu Asn Gln Ala Trp Ala Lys Ile His Phe Val Ile
405 410 415
Met Phe Val Gly Val Asn Met Thr Phe Phe Pro Gln His Phe Leu Gly
420 425 430
Leu Ser Gly Met Pro Arg Arg Tyr Ser Asp Tyr Pro Asp Ala Tyr Thr
435 440 445
Ala Trp Asn Thr Ile Ser Ser Met Gly Ser Phe Ile Ser Leu Thr Ala
450 455 460
Val Met Leu Met Ile Phe Ile Ile Trp Glu Ala Phe Ala Ser Lys Arg
465 470 475 480
Glu Val Ser Ala Val Glu Leu Thr Ser Thr Asn Leu Glu Trp Leu His
485 490 495
Gly Cys Pro Pro Pro Tyr His Thr Phe Glu Glu Pro Thr Tyr Ile Asn
500 505 510
Leu Lys
<210> 59
<211> 229
<212> PRT
<213> Sus scrofa
<400> 59
Met Ala Tyr Pro Phe Gln Leu Gly Phe Gln Asp Ala Thr Ser Pro Ile
1 5 10 15
Met Glu Glu Leu Leu His Phe His Asp His Thr Leu Met Ile Val Phe
20 25 30
Leu Ile Ser Ser Leu Val Leu Tyr Ile Ile Ser Leu Met Leu Thr Thr
35 40 45
Lys Leu Thr His Thr Ser Thr Met Asp Ala Gln Glu Val Glu Thr Ile
50 55 60
Trp Thr Ile Leu Pro Ala Ile Ile Leu Ile Leu Ile Ala Leu Pro Ser
65 70 75 80
Leu Arg Ile Leu Tyr Met Met Asp Glu Ile Asn Asn Pro Ala Leu Thr
85 90 95
Val Lys Thr Met Gly His Gln Trp Tyr Trp Ser Tyr Glu Tyr Thr Asp
100 105 110
Tyr Glu Asp Leu Thr Phe Asp Ser Tyr Met Ile Pro Thr Ser Asp Leu
115 120 125
Lys Pro Gly Glu Met Arg Leu Leu Glu Val Asp Asn Arg Val Val Leu
130 135 140
Pro Met Glu Met Thr Ile Arg Met Leu Val Ser Ser Glu Asp Val Leu
145 150 155 160
His Ser Trp Ala Val Pro Ser Leu Gly Leu Lys Thr Asp Ala Ile Pro
165 170 175
Gly Arg Leu Asn Gln Thr Thr Leu Met Ser Thr Arg Pro Gly Leu Tyr
180 185 190
Tyr Gly Gln Cys Ser Glu Ile Cys Gly Ser Asn His Ser Phe Met Pro
195 200 205
Ile Val Leu Glu Leu Val Pro Leu Lys Tyr Phe Glu Lys Trp Ser Thr
210 215 220
Ser Met Leu Thr Gly
225
<210> 60
<211> 169
<212> PRT
<213> Sus scrofa
<400> 60
Met Leu Ala Thr Arg Val Phe Asn Leu Ile Gly Arg Arg Ala Ile Ser
1 5 10 15
Thr Ser Val Cys Val Arg Ala His Gly Ser Val Val Lys Ser Glu Asp
20 25 30
Tyr Ala Leu Pro Val Tyr Val Asp Arg Arg Asp Tyr Pro Leu Pro Asp
35 40 45
Val Ala His Val Lys Asn Leu Ser Ala Ser Gln Lys Ala Leu Lys Glu
50 55 60
Lys Glu Lys Ala Ser Trp Ser Ser Leu Ser Met Asp Glu Lys Val Glu
65 70 75 80
Leu Tyr Arg Leu Lys Phe Asn Glu Ser Phe Ala Glu Met Asn Arg Ser
85 90 95
Thr Asn Glu Trp Lys Thr Ile Val Gly Thr Ala Leu Phe Phe Ile Gly
100 105 110
Phe Thr Ala Leu Ile Leu Ile Trp Glu Lys His Tyr Val Tyr Gly Pro
115 120 125
Ile Pro His Thr Phe Glu Glu Glu Trp Val Ala Lys Gln Thr Lys Arg
130 135 140
Met Leu Asp Met Lys Val Ala Pro Ile Gln Gly Phe Ser Ala Lys Trp
145 150 155 160
Asp Tyr Asp Lys Asn Glu Trp Lys Lys
165
<210> 61
<211> 152
<212> PRT
<213> Sus scrofa
<400> 61
Met Leu Gly Ala Ala Val Arg Arg Cys Ser Val Ala Ala Ala Ala Val
1 5 10 15
Ala Arg Ala Ser Pro Arg Gly Leu Leu His Pro Thr Pro Thr Pro Gly
20 25 30
Pro Ala Ala Ala Ile Gln Ser Ile Arg Cys Tyr Ser His Gly Ser His
35 40 45
Glu Thr Asp Glu Glu Phe Asp Ala Arg Trp Val Thr Tyr Phe Asn Lys
50 55 60
Pro Asp Ile Asp Ala Trp Glu Leu Arg Lys Gly Met Asn Thr Leu Val
65 70 75 80
Gly Tyr Asp Leu Val Pro Glu Pro Lys Ile Ile Asp Ala Ala Leu Arg
85 90 95
Ala Cys Arg Arg Leu Asn Asp Phe Ala Ser Ala Val Arg Ile Leu Glu
100 105 110
Val Val Lys Asp Lys Ala Gly Pro His Lys Glu Ile Tyr Pro Tyr Val
115 120 125
Ile Gln Glu Leu Arg Pro Thr Leu Asn Glu Leu Gly Ile Ser Thr Pro
130 135 140
Glu Glu Leu Gly Leu Asp Lys Val
145 150
<210> 62
<211> 129
<212> PRT
<213> Sus scrofa
<400> 62
Met Ala Ser Arg Leu Leu Arg Gly Ala Gly Ala Leu Ala Ala Gln Thr
1 5 10 15
Leu Arg Ala Arg Gly Pro Asn Gly Val Ala Val Val Arg Ser Met Ala
20 25 30
Ser Gly Gly Gly Val Pro Thr Asp Glu Glu Gln Ala Thr Gly Leu Glu
35 40 45
Arg Glu Val Met Met Ala Ala Arg Lys Gly Leu Asp Pro Tyr Asn Ile
50 55 60
Leu Ala Pro Lys Ala Ala Ser Gly Thr Lys Glu Asp Pro Asn Leu Val
65 70 75 80
Pro Ser Ile Thr Asn Lys Arg Ile Val Gly Cys Ile Cys Glu Glu Asp
85 90 95
Asn Ser Thr Val Ile Trp Phe Trp Val His Lys Gly Glu Thr Gln Arg
100 105 110
Cys Pro Ser Cys Gly Thr His Tyr Lys Leu Val Ser His Gln Leu Ala
115 120 125
His
<210> 63
<211> 109
<212> PRT
<213> Sus scrofa
<400> 63
Met Ala Ala Ala Ala Gly Ser Arg Val Ser Gly Leu Leu Arg Arg Ser
1 5 10 15
Gln Leu Gln Leu Arg Arg Pro Met Ser Ser Ser Ala His Gly Glu Glu
20 25 30
Gly Ser Ala Arg Met Trp Lys Thr Leu Thr Tyr Phe Val Ala Leu Pro
35 40 45
Gly Val Gly Val Ser Met Leu Asn Val Phe Leu Lys Ser His His Ala
50 55 60
Glu Glu Glu Arg Pro Glu Phe Val Pro Tyr Pro His Leu Arg Ile Arg
65 70 75 80
Ser Lys Pro Phe Pro Trp Gly Asp Gly Asn His Thr Leu Phe His Asn
85 90 95
Pro His Val Asn Pro Leu Pro Thr Gly Tyr Glu Asp Glu
100 105
<210> 64
<211> 86
<212> PRT
<213> Sus scrofa
<400> 64
Met Ala Glu Asp Ile Gln Thr Lys Ile Lys Asn Tyr Gln Thr Ala Pro
1 5 10 15
Phe Asp Ser Arg Phe Pro Asn Gln Asn Gln Thr Arg Asn Cys Trp Gln
20 25 30
Asn Tyr Leu Asp Phe His Arg Cys Glu Lys Ala Met Thr Ala Lys Gly
35 40 45
Gly Asp Val Ser Val Cys Glu Trp Tyr Arg Arg Val Tyr Lys Ser Phe
50 55 60
Cys Pro Ile Ser Trp Val Ser Ala Trp Asp Asp Arg Arg Ala Glu Gly
65 70 75 80
Thr Phe Pro Gly Lys Ile
85
<210> 65
<211> 76
<212> PRT
<213> Sus scrofa
<400> 65
Met Ala Thr Ser Ser Leu Thr Lys Pro Gln Met Arg Trp Pro Ser Gly
1 5 10 15
Pro Arg Arg Leu Arg Phe His Ile Val Gly Ala Phe Ile Val Ser Leu
20 25 30
Gly Val Ala Thr Phe Tyr Lys Phe Ala Val Ala Glu Pro Arg Lys Lys
35 40 45
Ala Tyr Ala Asp Phe Tyr Arg Asn Tyr Asp Ser Met Lys Asp Phe Glu
50 55 60
Glu Met Arg Lys Ala Gly Ile Phe Gln Ser Ala Lys
65 70 75
<210> 66
<211> 83
<212> PRT
<213> Sus scrofa
<400> 66
Met Leu Arg Asn Leu Leu Ala Leu Arg Gln Ile Ala Gln Arg Thr Ile
1 5 10 15
Ser Ser Ala Ser Arg Arg Gln Leu Glu Asn Lys Val Ala Glu Lys Gln
20 25 30
Lys Leu Phe Gln Glu Asp Asn Gly Ile Pro Val His Leu Lys Gly Gly
35 40 45
Ile Gly Asp Ala Phe Leu Tyr Arg Ala Thr Met Ile Leu Thr Val Gly
50 55 60
Gly Thr Ala Tyr Thr Ile Tyr Gln Leu Ala Val Ala Ser Leu Pro Lys
65 70 75 80
Lys Gln Asp
<210> 67
<211> 80
<212> PRT
<213> Sus scrofa
<400> 67
Met Phe Pro Leu Ala Lys Asn Ala Leu Ser Arg Leu Arg Val Arg Asn
1 5 10 15
Ile Gln Gln Thr Met Ala Arg Gln Asn His Gln Lys Arg Ala Pro Asp
20 25 30
Phe His Asp Lys Tyr Gly Asn Ala Ile Leu Ala Ser Gly Ala Thr Phe
35 40 45
Cys Val Ala Val Trp Ala Tyr Thr Ala Thr Gln Ile Gly Ile Glu Trp
50 55 60
Asn Leu Ser Pro Val Gly Arg Val Thr Pro Lys Glu Trp Arg Glu Glu
65 70 75 80
<210> 68
<211> 63
<212> PRT
<213> Sus scrofa
<400> 68
Met Leu Gly Gln Ser Ile Arg Arg Phe Thr Thr Ser Val Val Arg Arg
1 5 10 15
Ser His Tyr Glu Glu Gly Pro Gly Lys Asn Leu Pro Phe Ser Val Glu
20 25 30
Asn Lys Trp Arg Leu Leu Ala Met Met Thr Leu Tyr Phe Gly Ser Gly
35 40 45
Phe Ala Ala Pro Phe Phe Ile Val Arg His Gln Leu Leu Lys Lys
50 55 60
<210> 69
<211> 70
<212> PRT
<213> Sus scrofa
<400> 69
Met Leu Arg Leu Ala Pro Thr Val Arg Leu Leu Gln Ala Pro Leu Gly
1 5 10 15
Gly Trp Val Val Pro Lys Ala His Ile Tyr Ala Lys Pro Ala Arg Thr
20 25 30
Pro Thr Ser Pro Thr Glu Gln Ala Ile Gly Leu Ser Val Thr Phe Leu
35 40 45
Ser Phe Leu Ile Pro Ala Gly Trp Val Leu Ser His Leu Asp His Tyr
50 55 60
Lys Arg Ser Ser Ala Ala
65 70

Claims (12)

1.一种哺乳类呼吸链超级复合物蛋白，由一个NADH脱氢酶复合物I、两个细胞色素bc1复合物III和一个细胞色素c氧化酶复合物IV组成超级复合物I1III2IV1，超级复合物I1III2IV1长300埃，高190埃；在冷冻电镜三维结构中，超级复合物整体分辨率高于4埃；
其中NADH脱氢酶复合物I包含如下45个蛋白亚基：
蛋白1具有SEQ ID NO:1的蛋白序列；或者与SEQ ID NO:1具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:1具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白2具有SEQ ID NO:2的蛋白序列；或者与SEQ ID NO:2具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:2具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白3具有SEQ ID NO:3的蛋白序列；或者与SEQ ID NO:3具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:3具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白4具有SEQ ID NO:4的蛋白序列；或者与SEQ ID NO:4具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:4具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白5具有SEQ ID NO:5的蛋白序列；或者与SEQ ID NO:5具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:5具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白6具有SEQ ID NO:6的蛋白序列；或者与SEQ ID NO:6具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:6具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白7具有SEQ ID NO:7的蛋白序列；或者与SEQ ID NO:7具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:7具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白8具有SEQ ID NO:8的蛋白序列；或者与SEQ ID NO:8具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:8具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白9具有SEQ ID NO:9的蛋白序列；或者与SEQ ID NO:9具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:9具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白10具有SEQ ID NO:10的蛋白序列；或者与SEQ ID NO:10具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:10具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白11具有SEQ ID NO:11的蛋白序列；或者与SEQ ID NO:11具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:11具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白12具有SEQ ID NO:12的蛋白序列；或者与SEQ ID NO:12具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:12具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白13具有SEQ ID NO:13的蛋白序列；或者与SEQ ID NO:13具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:13具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白14具有SEQ ID NO:14的蛋白序列；或者与SEQ ID NO:14具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:14具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白15具有SEQ ID NO:15的蛋白序列；或者与SEQ ID NO:15具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:15具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白16具有SEQ ID NO:16的蛋白序列；或者与SEQ ID NO:16具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:16具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白17具有SEQ ID NO:17的蛋白序列；或者与SEQ ID NO:17具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:17具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白18具有SEQ ID NO:18的蛋白序列；或者与SEQ ID NO:18具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:18具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白19具有SEQ ID NO:19的蛋白序列；或者与SEQ ID NO:19具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:19具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白20具有SEQ ID NO:20的蛋白序列；或者与SEQ ID NO:20具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:20具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白21具有SEQ ID NO:21的蛋白序列；或者与SEQ ID NO:21具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:21具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白22具有SEQ ID NO:22的蛋白序列；或者与SEQ ID NO:22具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:22具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白23具有SEQ ID NO:23的蛋白序列；或者与SEQ ID NO:23具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:23具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白24具有SEQ ID NO:24的蛋白序列；或者与SEQ ID NO:24具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:24具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白25具有SEQ ID NO:25的蛋白序列；或者与SEQ ID NO:25具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:25具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白26具有SEQ ID NO:26的蛋白序列；或者与SEQ ID NO:26具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:26具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白27具有SEQ ID NO:27的蛋白序列；或者与SEQ ID NO:27具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:27具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白28具有SEQ ID NO:28的蛋白序列；或者与SEQ ID NO:28具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:28具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白29具有SEQ ID NO:29的蛋白序列；或者与SEQ ID NO:29具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:29具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白30具有SEQ ID NO:30的蛋白序列；或者与SEQ ID NO:30具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:30具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白31具有SEQ ID NO:31的蛋白序列；或者与SEQ ID NO:31具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:31具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白32具有SEQ ID NO:32的蛋白序列；或者与SEQ ID NO:32具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:32具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白33具有SEQ ID NO:33的蛋白序列；或者与SEQ ID NO:33具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:33具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白34具有SEQ ID NO:34的蛋白序列；或者与SEQ ID NO:34具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:34具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白35具有SEQ ID NO:35的蛋白序列；或者与SEQ ID NO:35具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:35具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白36具有SEQ ID NO:36的蛋白序列；或者与SEQ ID NO:36具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:36具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白37具有SEQ ID NO:37的蛋白序列；或者与SEQ ID NO:37具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:37具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白38具有SEQ ID NO:38的蛋白序列；或者与SEQ ID NO:38具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:38具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白39具有SEQ ID NO:39的蛋白序列；或者与SEQ ID NO:39具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:39具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白40具有SEQ ID NO:40的蛋白序列；或者与SEQ ID NO:40具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:40具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白41具有SEQ ID NO:41的蛋白序列；或者与SEQ ID NO:41具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:41具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白42具有SEQ ID NO:42的蛋白序列；或者与SEQ ID NO:42具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:42具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白43具有SEQ ID NO:43的蛋白序列；或者与SEQ ID NO:43具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:43具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白44具有SEQ ID NO:44的蛋白序列；或者与SEQ ID NO:44具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:44具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白45具有SEQ ID NO:45的蛋白序列；或者与SEQ ID NO:45具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:45具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
其中细胞色素bc1复合物III包含如下11个蛋白序列：
蛋白46具有SEQ ID NO:46的蛋白序列；或者与SEQ ID NO:46具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:46具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白47具有SEQ ID NO:47的蛋白序列；或者与SEQ ID NO:47具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:47具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白48具有SEQ ID NO:48的蛋白序列；或者与SEQ ID NO:48具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:48具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白49具有SEQ ID NO:49的蛋白序列；或者与SEQ ID NO:49具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:49具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白50具有SEQ ID NO:50的蛋白序列；或者与SEQ ID NO:50具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:50具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白51具有SEQ ID NO:51的蛋白序列；或者与SEQ ID NO:51具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:51具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白52具有SEQ ID NO:52的蛋白序列；或者与SEQ ID NO:52具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:52具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白53具有SEQ ID NO:53的蛋白序列；或者与SEQ ID NO:53具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:53具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白54具有SEQ ID NO:54的蛋白序列；或者与SEQ ID NO:54具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:54具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白55具有SEQ ID NO:55的蛋白序列；或者与SEQ ID NO:55具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:55具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；和
蛋白56具有SEQ ID NO:56的蛋白序列；或者与SEQ ID NO:56具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:56具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
其中细胞色素c氧化酶复合物IV包含如下13个蛋白序列：
蛋白57具有SEQ ID NO:57的蛋白序列；或者与SEQ ID NO:57具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:57具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白58具有SEQ ID NO:58的蛋白序列；或者与SEQ ID NO:58具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:58具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白59具有SEQ ID NO:59的蛋白序列；或者与SEQ ID NO:59具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:59具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白60具有SEQ ID NO:60的蛋白序列；或者与SEQ ID NO:60具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:60具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白61具有SEQ ID NO:61的蛋白序列；或者与SEQ ID NO:61具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:61具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白62具有SEQ ID NO:62的蛋白序列；或者与SEQ ID NO:62具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:62具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白63具有SEQ ID NO:63的蛋白序列；或者与SEQ ID NO:63具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:63具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白64具有SEQ ID NO:64的蛋白序列；或者与SEQ ID NO:64具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:64具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白65具有SEQ ID NO:65的蛋白序列；或者与SEQ ID NO:65具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:65具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白66具有SEQ ID NO:66的蛋白序列；或者与SEQ ID NO:66具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:66具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白67具有SEQ ID NO:67的蛋白序列；或者与SEQ ID NO:67具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:67具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；
蛋白68具有SEQ ID NO:68的蛋白序列；或者与SEQ ID NO:68具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:68具有一个或几个氨基酸的取代、缺失或替换的蛋白序列；和
蛋白69具有SEQ ID NO:69的蛋白序列；或者与SEQ ID NO:69具有至少95％氨基酸序列同一性的蛋白；或者与SEQ ID NO:69具有一个或几个氨基酸的取代、缺失或替换的蛋白序列。
2.权利要求1所述的蛋白，其中哺乳动物来源为Susscrofa。
3.权利要求1或2所述的蛋白，其中所述的同一性为至少70、80、90、95或99％同一性。
4.前述任一项权利要求所述的蛋白，其80％-88％的氨基酸的侧链位置可以准确的定位，90％-95％蛋白氨基酸的主链可以准确定位。
5.前述任一项权利要求所述的蛋白，其中复合物I包含78个α跨膜螺旋、复合物III包含28个α跨膜螺旋、复合物IV包含28个α跨膜螺旋。
6.前述任一项权利要求所述的蛋白，其中各氨基酸侧链冲撞分数(Clashsore)＝22、拉氏图统计(Ramachandran outliers)＝2.6％、侧链统计(Sidechain outliers)＝1.4％。
7.前述任一项权利要求所述的蛋白，其中NADH脱氢酶复合物I包含如下45个蛋白质序列：SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3、SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ IDNO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ IDNO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ IDNO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ IDNO:42、SEQ ID NO:43、SEQ ID NO:44和SEQ ID NO:45；
细胞色素bc1复合物III包含如下11个蛋白质序列：SEQ ID NO:46、SEQ ID NO:47、SEQID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQID NO:54、SEQ ID NO:55和SEQ ID NO:56；
细胞色素c氧化酶复合物IV包含如下13个蛋白质序列：SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68和SEQ ID NO:69。
8.前述任一项权利要求所述的蛋白，其中
NADH脱氢酶复合物I由如下45个蛋白质序列组成：SEQ ID NO:1、SEQ ID NO:2、SEQ IDNO:3、SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ IDNO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ IDNO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ IDNO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ IDNO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44和SEQID NO:45；
细胞色素bc1复合物III由如下11个蛋白质序列组成：SEQ ID NO:46、SEQ ID NO:47、SEQID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQID NO:54、SEQ ID NO:55和SEQ ID NO:56；
细胞色素c氧化酶复合物IV由如下13个蛋白质序列组成：SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ IDNO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68和SEQ ID NO:69。
9.一种制备权利要求1-8中任一项所述蛋白的方法，该方法包括以下步骤：
A.线粒体的提取；
B.从线粒体中抽提呼吸链超级复合物蛋白；
C.纯化步骤B获得的超级复合物蛋白；
D.对步骤C的纯化产物进行BN-PAGE检测；和
E.进行凝胶排阻层析。
10.根据权利要求9所述的方法，其中在所述的步骤E中，以三羟基氨基甲烷、氯化钾和洋地黄皂苷为溶质配制层析缓冲液，将含有超级复合物的样品载入到凝胶排阻层析柱上，进行层析，并收取峰尖。
11.根据权利要求10所述的方法，其中层析缓冲液具有7-8的pH值，优选为7.3-7.5的pH值。
12.根据权利要求10或11所述的方法，其中所述的峰尖是采用的AKTA purifier层析仪器，层析柱为Superose 6 10/300GL，收集在280nm波长下检测到的蛋白峰的最高值。
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