CN107691305A - An experimental device for the determination of fish activity and metabolic rate under low oxygen - Google Patents
An experimental device for the determination of fish activity and metabolic rate under low oxygen Download PDFInfo
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- 239000001301 oxygen Substances 0.000 title claims abstract description 49
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 49
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 48
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 25
- 230000000694 effects Effects 0.000 title claims abstract description 12
- 230000037323 metabolic rate Effects 0.000 title claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 64
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 35
- 238000012360 testing method Methods 0.000 claims abstract description 25
- 239000000523 sample Substances 0.000 claims abstract description 10
- 239000011261 inert gas Substances 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 206010021143 Hypoxia Diseases 0.000 claims description 15
- 238000005259 measurement Methods 0.000 claims description 14
- 230000007954 hypoxia Effects 0.000 claims description 10
- 238000005192 partition Methods 0.000 claims description 5
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims description 4
- 229920005372 Plexiglas® Polymers 0.000 claims description 4
- 239000007789 gas Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 238000013461 design Methods 0.000 abstract description 3
- 230000006399 behavior Effects 0.000 description 14
- 230000006641 stabilisation Effects 0.000 description 7
- 238000011105 stabilization Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000001146 hypoxic effect Effects 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229940110728 nitrogen / oxygen Drugs 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Zoology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
Description
技术领域technical field
本发明涉及一种鱼类实验装置。The invention relates to a fish experiment device.
背景技术Background technique
环境低氧是水生生态系统中常见的自然现象，而人类活动造成的全球气候变暖、水体富营养化、环境污染等进一步加剧了水体中的低氧现象，常常给渔业造成大量损失。有关鱼类耐低氧的相关研究对于降低渔业损失至关重要，也一直是国际研究热点领域，但相关试验设备一直受限：已有试验设备设计过于简单，未能充分解决水体中溶氧的均匀混合问题，使得试验精度难以控制；在测定过程中无法避免气泡的产生，使得鱼类在低氧下的行为反应很难观测。另外，这类装置往往固定体积，日常代谢测定过程中只能测定特定大小的试验鱼，使得相关研究十分受限。Environmental hypoxia is a common natural phenomenon in aquatic ecosystems, and human activities caused by global warming, water eutrophication, and environmental pollution have further aggravated the hypoxic phenomenon in water bodies, often causing large losses to fisheries. Research on fish hypoxia tolerance is crucial to reducing fishery losses, and has always been an international research hotspot, but the relevant test equipment has been limited: the existing test equipment design is too simple and cannot fully solve the problem of dissolved oxygen in water. The problem of uniform mixing makes it difficult to control the accuracy of the test; the generation of air bubbles cannot be avoided during the measurement process, making it difficult to observe the behavioral response of fish under low oxygen. In addition, such devices often have a fixed volume, and only test fish of a specific size can be measured in the daily metabolic measurement process, which makes related research very limited.
发明内容Contents of the invention
本发明的目的是提供一种用于低氧下鱼类活跃性和代谢率测定的试验装置。The purpose of the present invention is to provide a kind of test device that is used for fish activity and metabolic rate measurement under hypoxia.
为了实现上述目的，采用以下技术方案：In order to achieve the above purpose, the following technical solutions are adopted:
一种用于低氧下鱼类活跃性和代谢率测定的试验装置，包括混合槽、稳定槽和测定槽，其特征在于：混合槽和稳定槽的顶部均设置有带有单向气阀的密封膜，混合槽和稳定槽的上部均设置有溶氧探头，混合槽的底部设置有惰性气体出口，在混合槽的底部与稳定槽的底部通过连通水管连接；稳定槽的底部中央设置有带有电机的螺旋桨，水泵将稳定槽内的水从稳定槽的底部排入测定槽的底部；测定槽底部设置有倾斜的多孔底板；在测定槽的底部设置有溶氧仪；在测定槽的上部设置有盖板，盖板上连有带有溶氧仪的管道，将测定槽内的水排出。A test device for measuring the activity and metabolic rate of fish under low oxygen, comprising a mixing tank, a stabilizing tank and a measuring tank, characterized in that: the tops of the mixing tank and the stabilizing tank are equipped with one-way air valves The sealing film, the upper part of the mixing tank and the stabilizing tank are equipped with dissolved oxygen probes, the bottom of the mixing tank is provided with an inert gas outlet, and the bottom of the mixing tank is connected with the bottom of the stabilizing tank through a connecting water pipe; the center of the bottom of the stabilizing tank is provided with a There is a propeller with a motor, and the water pump discharges the water in the stable tank from the bottom of the stable tank to the bottom of the measuring tank; the bottom of the measuring tank is provided with an inclined porous bottom plate; the bottom of the measuring tank is equipped with a dissolved oxygen meter; the upper part of the measuring tank A cover plate is provided, and a pipe with a dissolved oxygen meter is connected to the cover plate to discharge the water in the measuring tank.
多孔底板为白色。The perforated bottom plate is white.
混和槽中水位高于稳定槽。The water level in the mixing tank is higher than that in the stabilization tank.
测定槽包括行为观察室和观察室下方的空间，多孔底板与水平面呈10°；上盖为透明有机玻璃板，利于摄像机对试验鱼的行为拍摄，盖板与摄像机的安装也与水平面呈10°。The measurement tank includes the behavior observation room and the space below the observation room. The porous bottom plate is 10° to the horizontal plane; the upper cover is a transparent plexiglass plate, which is beneficial for the camera to shoot the behavior of the test fish. The installation of the cover plate and the camera is also 10° to the horizontal plane. .
溶氧测量瓶为圆柱形，内部由一隔板将测量瓶内部分为大小不等的两个半圆柱空间，上方空间更大，溶氧探头伸入瓶中，水经过溶氧探头后流经下半圆柱空间，从出水口流出。The dissolved oxygen measuring bottle is cylindrical, and the inside of the measuring bottle is divided into two semi-cylindrical spaces of different sizes by a partition. The upper space is larger. The dissolved oxygen probe extends into the bottle, and the water flows through the dissolved oxygen probe The lower semi-cylindrical space flows out from the water outlet.
混和槽、稳定槽和测定槽三个水槽之间相互连接，混和槽整体最长，稳定槽和测定槽置于支架之上，三个水槽构成一整体，且上沿同高。The mixing tank, the stabilizing tank and the measuring tank are connected to each other, the mixing tank is the longest overall, the stabilizing tank and the measuring tank are placed on the support, the three water tanks form a whole, and the upper edges are at the same height.
本发明可使进入行为观察室的低氧水混合均匀，避免局部溶氧偏高或偏低对结果造成影响，通过多处的设计将产生的气泡排除，避免气泡过多造成低氧下试验鱼的行为无法观察，行为观察室的大小可调节，满足不同程度低氧和不同大小试验鱼的测定要求，利用连通器原理，仅使用一台水泵就可以使整个装置的水运行起来，节约水泵和电能。The invention can evenly mix the hypoxic water entering the behavior observation room, avoid the influence of high or low local dissolved oxygen on the results, eliminate the generated air bubbles through the design of multiple places, and avoid excessive air bubbles causing the test fish under hypoxia The behavior of the fish cannot be observed, and the size of the behavior observation room can be adjusted to meet the measurement requirements of different degrees of hypoxia and different sizes of test fish. Using the principle of the connecting device, only one water pump can make the water in the whole device run, saving water pumps and electrical energy.
附图说明Description of drawings
图1为具体实施例的结构示意图；Fig. 1 is the structural representation of specific embodiment;
图2为测量瓶的结构示意图；Fig. 2 is the structural representation of measuring bottle;
图3为测量瓶的俯视图。Figure 3 is a top view of the measuring bottle.
图中，1为氮气瓶；2为气阀；3为导气管；4为充气泵；5为三角形支架；6为溶氧混和槽；7为回水导管；8为溶氧探头；9为溶氧稳定槽；10为塑料薄膜；11为单向气阀；12为溶氧仪；13为溶氧仪；14为摄像机；15为行为观察室；16为测定槽；17为透明有机玻璃盖板；18为管道；19为充气砂头；20为多孔隔板；21为连通水管；22为电机；23为螺旋桨；24为水泵；25为入水口；26为白色多孔底板；27为支架；28为管道；29为溶氧测量瓶；30为溶氧仪；31为溶氧仪；32为溶氧测量瓶入水口；33为隔板；35为溶氧测量瓶出水口。In the figure, 1 is a nitrogen cylinder; 2 is an air valve; 3 is an air guide tube; 4 is an air pump; 5 is a triangular bracket; 6 is a dissolved oxygen mixing tank; 7 is a return conduit; Oxygen stabilization tank; 10 is a plastic film; 11 is a one-way air valve; 12 is a dissolved oxygen meter; 13 is a dissolved oxygen meter; 14 is a video camera; 15 is a behavior observation room; 16 is a measuring tank; 17 is a transparent plexiglass cover ; 18 is a pipeline; 19 is an inflatable sand head; 20 is a porous partition; 21 is a connected water pipe; 22 is a motor; 23 is a propeller; 24 is a water pump; 25 is a water inlet; 26 is a white porous bottom plate; 29 is a dissolved oxygen measuring bottle; 30 is a dissolved oxygen meter; 31 is a dissolved oxygen meter; 32 is a water inlet of a dissolved oxygen measuring bottle; 33 is a partition; 35 is a water outlet of a dissolved oxygen measuring bottle.
具体实施方式detailed description
下面结合附图和具体实施例对本发明做进一步说明。The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.
如图1至图3所示，一种用于低氧下鱼类活跃性和代谢率测定的试验装置，包括混合槽、稳定槽和测定槽，混合槽和稳定槽的顶部均设置有带有单向气阀的密封膜，混合槽和稳定槽的上部均设置有溶氧探头，混合槽的底部设置有惰性气体出口，在混合槽的底部与稳定槽的底部通过连通水管连接；稳定槽的底部中央设置有带有电机的螺旋桨，水泵将稳定槽内的水从稳定槽的底部排入测定槽的底部；测定槽底部设置有倾斜的多孔底板；在测定槽的底部设置有溶氧仪；在测定槽的上部设置有盖板，盖板上连有带有溶氧仪的管道，将测定槽内的水排出。As shown in Figures 1 to 3, a test device for fish activity and metabolic rate determination under low oxygen includes a mixing tank, a stabilizing tank and a measuring tank. The tops of the mixing tank and the stabilizing tank are equipped with The sealing film of the one-way air valve, the upper part of the mixing tank and the stabilizing tank are provided with dissolved oxygen probes, the bottom of the mixing tank is provided with an inert gas outlet, and the bottom of the mixing tank is connected with the bottom of the stabilizing tank through a connecting water pipe; There is a propeller with a motor in the center of the bottom, and the water pump discharges the water in the stabilization tank from the bottom of the stabilization tank to the bottom of the measurement tank; the bottom of the measurement tank is provided with an inclined porous bottom plate; a dissolved oxygen meter is installed at the bottom of the measurement tank; A cover plate is arranged on the upper part of the measuring tank, and a pipeline with a dissolved oxygen meter is connected on the cover plate to discharge the water in the measuring tank.
具体的，如图1所示，本实施例包括从左至右包括混合槽6、稳定槽9和测定槽16三个试验水槽。向混和槽6底部充入氮气使水中溶氧降低至设定值附近。稳定槽中有水泵24向测定槽16中抽水，因此混和槽6中水位高于稳定槽9，二者底部通过管道21连接，使得混和槽6中的低氧水可通过连通器原理进入稳定槽9；低氧水在稳定槽9中通过螺旋桨23的搅动进一步混合均匀，由底部水泵24将水抽入测定槽（行为观察室15底板26下方的空间），再通过行为观察室15的多孔底板26均匀进入观察室15；之后水通过行为观察室上盖17的开口进入管道18，经过溶氧测量瓶29，通过管道28和7回到混和槽6，实现水体循环。混和槽6中的溶氧通过充入氮气和空气进行双向调节，进一步提高溶氧控制精度；混和槽6中水从下方流出，通过管道从上方流入，使得水流方向与气泡方向相反，可利于提高氮气/氧气与水的接触；混和槽6和稳定槽9均覆盖有透明薄膜10，阻断水槽中的水与空气中水进行气体交换，薄膜中部安装一单向气阀11，使得水槽中气体可通过单向阀排除，但外界空气无法进入；混和槽6与稳定槽9底部通过管道21连接，通过稳定槽中水泵24的作用，使得混和槽6中水位总高于稳定槽9，混和槽6中水可通过连通器原理自动流入稳定槽；稳定槽9底部安装螺旋桨23，通过螺旋桨23的选择使稳定槽9中的低氧水混合均匀；测定槽16包括行为观察室15和观察室下方的空间组成，观察室多孔底板26与水平面呈10°的角度；上盖为透明有机玻璃板盖板17，利于摄像机14对试验鱼的行为拍摄，上盖与摄像机14的安装也与水平面呈10°的角度；上盖最高处（最右端）安装有出水口，方便试验过程中观察室15内气泡的排除；Specifically, as shown in FIG. 1 , this embodiment includes three test water tanks including a mixing tank 6 , a stabilizing tank 9 and a measuring tank 16 from left to right. Nitrogen is charged to the bottom of the mixing tank 6 to reduce the dissolved oxygen in the water to near the set value. There is a water pump 24 in the stabilizing tank to pump water into the measuring tank 16, so the water level in the mixing tank 6 is higher than that of the stabilizing tank 9, and the bottom of the two is connected by a pipeline 21, so that the hypoxic water in the mixing tank 6 can enter the stabilizing tank through the principle of a connector 9. The hypoxic water is further mixed evenly by the agitation of the propeller 23 in the stability tank 9, and the water is pumped into the measurement tank (the space below the bottom plate 26 of the behavior observation room 15) by the bottom water pump 24, and then passes through the porous bottom plate of the behavior observation room 15 26 evenly enters the observation chamber 15; then the water enters the pipeline 18 through the opening of the upper cover 17 of the behavior observation chamber, passes through the dissolved oxygen measuring bottle 29, and returns to the mixing tank 6 through the pipelines 28 and 7 to realize water circulation. The dissolved oxygen in the mixing tank 6 is bidirectionally adjusted by filling nitrogen and air to further improve the control accuracy of dissolved oxygen; the water in the mixing tank 6 flows out from the bottom and flows in from the top through the pipe, so that the direction of the water flow is opposite to the direction of the air bubbles, which is beneficial to improve The contact between nitrogen/oxygen and water; the mixing tank 6 and the stabilizing tank 9 are covered with a transparent film 10, which blocks the gas exchange between the water in the tank and the water in the air, and a one-way air valve 11 is installed in the middle of the film to make the gas in the tank It can be excluded by a one-way valve, but outside air cannot enter; the mixing tank 6 is connected to the bottom of the stabilizing tank 9 through a pipeline 21, and through the action of the water pump 24 in the stabilizing tank, the water level in the mixing tank 6 is always higher than the stabilizing tank 9, and the mixing tank 6. Reclaimed water can automatically flow into the stabilization tank through the principle of a connector; a propeller 23 is installed at the bottom of the stabilization tank 9, and the hypoxic water in the stabilization tank 9 can be mixed evenly through the selection of the propeller 23; the measurement tank 16 includes a behavior observation room 15 and the bottom of the observation room Composition of space, the perforated bottom plate 26 of the observation chamber is at an angle of 10° to the horizontal plane; the upper cover is a transparent plexiglass plate cover plate 17, which is beneficial to the camera 14 to photograph the behavior of the test fish, and the installation of the upper cover and the video camera 14 is also at an angle of 10° to the horizontal plane. °; the highest part of the upper cover (the rightmost end) is equipped with a water outlet to facilitate the elimination of air bubbles in the observation room 15 during the test;
观察室上盖17的位置可在测定槽中上下调整，用以改变行为观察室的体积，方便测量不同大小的试验鱼。The position of the observation chamber loam cake 17 can be adjusted up and down in the measuring tank, in order to change the volume of the behavior observation chamber, convenient to measure test fish of different sizes.
行为观察室15底部为多孔的白色底板26，用以增加试验鱼与背景的反差；孔在底板上分布均匀，使得水均匀的通过观察室底部空间进入观察室。The bottom of the behavior observation chamber 15 is a porous white bottom plate 26, which is used to increase the contrast between the test fish and the background; the holes are evenly distributed on the bottom plate, so that water can evenly enter the observation chamber through the bottom space of the observation chamber.
混和槽6、稳定槽9和测定槽16三个水槽之间相互连接，混和槽6更高，置于地面，稳定槽9和测定槽16置于支架26之上，三个水槽构成一整体，且上沿同高。The mixing tank 6, the stabilizing tank 9 and the measuring tank 16 are connected to each other, the mixing tank 6 is higher and placed on the ground, the stabilizing tank 9 and the measuring tank 16 are placed on the support 26, and the three tanks form a whole. And the upper edge is the same height.
如图2和图3所示，溶氧测量瓶29为圆柱形，内空，有密封盖。内部由一隔板22将测量瓶29内部分为上下（测量瓶29水平放置）大小不等的两个半圆柱空间，上方空间更大，溶氧探头8深入其中，用以监测从观察室15出水的溶氧；水经过溶氧探头8后流经下半圆柱空间，从出水口流出；As shown in Figure 2 and Figure 3, the dissolved oxygen measuring bottle 29 is cylindrical, hollow inside, and has a sealed cover. The inside of the measuring bottle 29 is divided into two semi-cylindrical spaces of different sizes by a partition 22 (the measuring bottle 29 is placed horizontally). Dissolved oxygen in the water outlet; water flows through the lower semi-cylindrical space after passing through the dissolved oxygen probe 8, and flows out from the water outlet;
混和槽6、稳定槽9中各安装一溶氧探头8，用以监测和控制进入测定槽16中水的溶氧；观察室底部安装一溶氧探头，用以测定水在进入观察室15之前的溶氧，溶氧仪30和31的差值即为试验鱼所消耗，可用于计算试验鱼的代谢率。A dissolved oxygen probe 8 is respectively installed in the mixing tank 6 and the stabilizing tank 9 to monitor and control the dissolved oxygen entering the water in the measuring tank 16; The dissolved oxygen, the difference between the dissolved oxygen meter 30 and 31 is the consumption of the test fish, which can be used to calculate the metabolic rate of the test fish.
Claims (6)
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| CN201711090336.1A CN107691305A (en) | 2017-11-08 | 2017-11-08 | An experimental device for the determination of fish activity and metabolic rate under low oxygen |
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| CN201711090336.1A CN107691305A (en) | 2017-11-08 | 2017-11-08 | An experimental device for the determination of fish activity and metabolic rate under low oxygen |
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| CN109644928A (en) * | 2019-01-24 | 2019-04-19 | 中国水产科学研究院南海水产研究所 | A kind of aquatic animal intermittent hypoxia experimental device and control method thereof |
| CN111480608A (en) * | 2020-03-19 | 2020-08-04 | 菏泽学院 | Simple and efficient pelteobagrus vachelli hypoxia model experiment system and experiment method |
| CN111493002A (en) * | 2020-03-19 | 2020-08-07 | 菏泽学院 | Device for measuring activity and metabolic rate of pelteobagrus vachelli under low oxygen |
| CN115053854A (en) * | 2022-06-21 | 2022-09-16 | 南京师范大学 | Leiocassis longirostris growth condition monitoring device under low oxygen environment |
| CN116171913A (en) * | 2023-03-29 | 2023-05-30 | 中国水产科学研究院淡水渔业研究中心 | Device and method for screening fresh water snail living bodies |
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| CN109644928A (en) * | 2019-01-24 | 2019-04-19 | 中国水产科学研究院南海水产研究所 | A kind of aquatic animal intermittent hypoxia experimental device and control method thereof |
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