CN107653726A - A kind of enzymatic beating process - Google Patents
A kind of enzymatic beating process Download PDFInfo
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- CN107653726A CN107653726A CN201711048476.2A CN201711048476A CN107653726A CN 107653726 A CN107653726 A CN 107653726A CN 201711048476 A CN201711048476 A CN 201711048476A CN 107653726 A CN107653726 A CN 107653726A
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- laccase
- enzymatic
- beating
- beating process
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
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Abstract
The present invention relates to pulp technology for making paper, more particularly to a kind of enzymatic beating process, comprise the following steps：The first step, the first laccase, the second laccase are modified respectively, obtain modified the first laccase and the second laccase；Second step, takes needlebush pulpboard to be discongested with hydrabrusher, then stirs, and then adds the first laccase and nano-bentonite suspension, reacts 2 hours, is then beaten, and the first laccase dosage is starched for 0.3kg/ tons；3rd step, takes leaf wood pulpboard to be discongested with hydrabrusher, then stirs, and then adds the second laccase and nano-bentonite suspension, reacts 2 hours, is then beaten, and the second laccase dosage is starched for 0.3kg/ tons；4th step, softwood pulp and hardwood pulp are mixed, complex enzyme is then added and is handled, be then sent into fiberizer and be beaten.Which solving energy consumption in existing enzymatic beating process reduces few technical problem.
Description
Technical field
The present invention relates to pulp technology for making paper, more particularly to a kind of enzymatic beating process.
Background technology
China Patent No.：201710500144.7 disclose a kind of method of biology enzyme auxiliary mashing, are related to paper technology neck
Domain, the raw material used in the methods of beating is 100% true qualities needlebush pulpboard, is comprised the following steps：(1) it is by mass fraction
100% true qualities needlebush pulpboard carries out discongesting for certain time with hydrabrusher, is then stirred with determining rotating speed；(2)
A certain amount of enzyme and nano-bentonite suspension will be added in the slurry discongested, at a certain temperature processing a period of time, so
Inactivation treatment is carried out to slurry afterwards；(3) slurry handled well is beaten through PFI fiberizers.The patent utilization biology enzyme pair
Slurry is pre-processed, and can be promoted the water suction profit and fibrillating degree of fiber, be improved beatability, still
Reduction is but not much actually on beating energy consumption.But reducing beating energy consumption using enzyme has broader practice prospect,
The applicant is directed to the paper pulp fiber using mashing enzyme pretreatment copy paper always, it is kept paper same physical properties
Under the premise of, beating energy consumption is reduced, so as to reduce production cost.
The content of the invention
Therefore, for it is above-mentioned the problem of, the present invention proposes that a kind of beating energy consumption is low, beatability is good, fibrosis are high
Enzymatic beating process.Which solving energy consumption in existing enzymatic beating process reduces few technical problem.
To achieve the above object, present invention employs following technical scheme：A kind of enzymatic beating process, including following step
Suddenly：
The first step, the first laccase, the second laccase are modified respectively, obtain the first modified laccase and the second paint
Enzyme；
Second step, takes needlebush pulpboard to be discongested with hydrabrusher, then stirs, and then adds the first laccase with receiving
Rice bentonite suspension, temperature control are reacted 2 hours, are then beaten, the beating degree after mashing is at 80 ° at 40~50 DEG C
More than SR, the first laccase dosage are starched for 0.3kg/ tons；
3rd step, takes leaf wood pulpboard to be discongested with hydrabrusher, then stirs, and then adds the second laccase with receiving
Rice bentonite suspension, temperature control are reacted 2 hours, are then beaten, the beating degree after mashing is at 80 ° at 40~50 DEG C
More than SR, the second laccase dosage are starched for 0.3kg/ tons；
4th step, softwood pulp and hardwood pulp are mixed, complex enzyme is then added and is handled, be then sent into fiberizer
Middle mashing, the beating degree after mashing is in 80 ° of more than SR.
Further, the weight consumption ratio of the weight consumption of the needlebush pulpboard and leaf wood pulpboard is 6:4.
Further, the modifying process of the first laccase is to take the former laccase aqueous solution to add EDC hydrochloric acid saline solution and L- third
Aminoacyl amine aqueous solution is reacted, and is then dialysed with addition citric acid solution.
Further, the modifying process of the second laccase is to take the former laccase aqueous solution to add thiourea dioxide water solution and two
The methyl sulfoxide aqueous solution is reacted, and is then added citric acid solution and is dialysed.
Further, the complex enzyme by following parts by weight material composition：Zytase 20~25, hemicellulase 10
~15, cellulase 15~20, glutamic acid 15~20, the pH value of the complex enzyme are controlled 4.5~5.5.
Further, in manufacturing process, the buffer solution formed using citric acid monohydrate and disodium hydrogen phosphate dodecahydrate is adjusted
Save pH value.
By using preceding solution, the beneficial effects of the invention are as follows：This enzymatic beating process by the first laccase with
The modification of second laccase, increase substantially both activity, and activity also improves a lot, and laccase can be reduced
Usage amount, it is possible to increase the effect of mashing, and complex enzyme is added, paper product matter can be improved into, reduce long stapled dosage, saved
About cost, the wire-dividing broom purification of fiber can be improved, save energy consumption, extend diaphragm service life, improve mashing ability；Further
, glutamic acid is added among complex enzyme, the quality decay of aftergrowth fiber can be suppressed from source, production cost is low to be adapted to
Applied to industrial production, it is possible to increase the reuse number of regenerated fiber, this will bring huge economic benefit to paper industry.
Embodiment
In conjunction with the present invention is further described with embodiment.
The present embodiment provides a kind of enzymatic beating process, comprises the following steps：
The first step, the first laccase, the second laccase are modified respectively, obtain the first modified laccase and the second paint
Enzyme；
Second step, takes needlebush pulpboard to be discongested with hydrabrusher, then stirs, and then adds the first laccase with receiving
Rice bentonite suspension, temperature control are reacted 2 hours, are then beaten, the beating degree after mashing is at 80 ° at 40~50 DEG C
More than SR, the first laccase dosage are starched for 0.3kg/ tons；
3rd step, takes leaf wood pulpboard to be discongested with hydrabrusher, then stirs, and then adds the second laccase with receiving
Rice bentonite suspension, temperature control are reacted 2 hours, are then beaten, the beating degree after mashing is at 80 ° at 40~50 DEG C
More than SR, the second laccase dosage are starched for 0.3kg/ tons；
4th step, softwood pulp and hardwood pulp are mixed, complex enzyme is then added and is handled, be then sent into fiberizer
Middle mashing, the beating degree after mashing is in 80 ° of more than SR.
The weight consumption of the needlebush pulpboard is 6 with the weight consumption ratio of leaf wood pulpboard:4.
The modifying process of first laccase is to take the former laccase aqueous solution to add EDC hydrochloric acid saline solution and L- alanyl aqueous amines
Solution is reacted, and is then dialysed with addition citric acid solution.The modifying process of second laccase is to take the former laccase aqueous solution
Add thiourea dioxide water solution and dimethyl sulphoxide aqueous solution is reacted, then add citric acid solution and carry out thoroughly
Analysis.
The complex enzyme by following parts by weight material composition：Zytase 20, hemicellulase 10, cellulase 15,
Glutamic acid 15, the pH value of the complex enzyme are controlled 4.5~5.5.In manufacturing process, using citric acid monohydrate and 12 hydration phosphorus
The buffer solution regulation pH value of sour disodium hydrogen composition.
Above-mentioned complex enzyme can also be by the material composition of lower parts by weight：Zytase 25, hemicellulase 15, cellulase
20th, glutamic acid 20, the pH value of the complex enzyme are controlled 4.5~5.5.In manufacturing process, using citric acid monohydrate and 12 water
Close the buffer solution regulation pH value of disodium hydrogen phosphate composition.
Above-mentioned complex enzyme can also be by the material composition of lower parts by weight：Zytase 22, hemicellulase 12, cellulase
17th, glutamic acid 17, the pH value of the complex enzyme are controlled 4.5~5.5.In manufacturing process, using citric acid monohydrate and 12 water
Close the buffer solution regulation pH value of disodium hydrogen phosphate composition.
This enzymatic beating process increases substantially both activity by the modification of the first laccase and the second laccase, and
And activity also improves a lot, and the usage amount of laccase can be reduced, it is possible to increase the effect of mashing, and add compound
Enzyme, paper product matter can be improved into, reduce long stapled dosage, it is cost-effective, the wire-dividing broom purification of fiber can be improved, save energy
Consumption, extend diaphragm service life, improve mashing ability；Further, glutamic acid is added among complex enzyme, can be pressed down from source
The quality decay of preparation, regeneration string, production cost is low to be suitably applied industrial production, it is possible to increase the reuse of regenerated fiber
Number, this will bring huge economic benefit to paper industry.
Although specifically showing and describing the present invention with reference to preferred embodiment, those skilled in the art should be bright
In vain, do not departing from the spirit and scope of the present invention that appended claims are limited, in the form and details can be right
The present invention makes a variety of changes, and is protection scope of the present invention.
Claims (6)
1. a kind of enzymatic beating process, it is characterised in that comprise the following steps：
The first step, the first laccase, the second laccase are modified respectively, obtain modified the first laccase and the second laccase；
Second step, takes needlebush pulpboard to be discongested with hydrabrusher, then stirs, and it is swollen with nanometer then to add the first laccase
Moisten native suspension, temperature control at 40~50 DEG C, react 2 hours, be then beaten, the beating degree after mashing 80 ° of SR with
On, the first laccase dosage is starched for 0.3kg/ tons；
3rd step, takes leaf wood pulpboard to be discongested with hydrabrusher, then stirs, and it is swollen with nanometer then to add the second laccase
Moisten native suspension, temperature control at 40~50 DEG C, react 2 hours, be then beaten, the beating degree after mashing 80 ° of SR with
On, the second laccase dosage is starched for 0.3kg/ tons；
4th step, softwood pulp and hardwood pulp are mixed, complex enzyme is then added and is handled, be then sent into fiberizer and beat
Slurry, the beating degree after mashing is in 80 ° of more than SR.
A kind of 2. enzymatic beating process according to claim 1, it is characterised in that：The weight consumption of the needlebush pulpboard
Weight consumption ratio with leaf wood pulpboard is 6:4.
A kind of 3. enzymatic beating process according to claim 1, it is characterised in that：The modifying process of first laccase is to take
The former laccase aqueous solution adds EDC hydrochloric acid saline solution and the L- alanimamides aqueous solution is reacted, and is then delayed with addition citric acid
Rush solution dialysis.
A kind of 4. enzymatic beating process according to claim 1, it is characterised in that：The modifying process of second laccase is to take
The former laccase aqueous solution adds thiourea dioxide water solution and dimethyl sulphoxide aqueous solution is reacted, and then adds lemon acid buffering
Solution is dialysed.
A kind of 5. enzymatic beating process according to claim 1, it is characterised in that：The complex enzyme is by following parts by weight
Material composition：Zytase 20~25, hemicellulase 10~15, cellulase 15~20, glutamic acid 15~20, it is described compound
The pH value of enzyme is controlled 4.5~5.5.
A kind of 6. enzymatic beating process according to claim 5, it is characterised in that：In manufacturing process, using citrate hydrate
The buffer solution regulation pH value of acid and disodium hydrogen phosphate dodecahydrate composition.
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| CN201711048476.2A CN107653726A (en) | 2017-10-31 | 2017-10-31 | A kind of enzymatic beating process |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111424448A (en) * | 2020-03-30 | 2020-07-17 | 长沙瑞福尼新材料科技有限公司 | Preparation process of leaf biomechanical pulp |
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|---|---|---|---|---|
| US20010018956A1 (en) * | 1996-08-13 | 2001-09-06 | Consortium Fur Elektrochemische Industrie Gmbh | Multi-component system for modifying, degrading or bleaching lignin, lignin-containing materials or similar substances and processes for its use |
| CN101864688A (en) * | 2010-05-27 | 2010-10-20 | 岳阳纸业股份有限公司 | Pulping method of sulphate chemical pulp |
| CN102978986A (en) * | 2012-11-27 | 2013-03-20 | 天津智展科技有限公司 | Method for preparing paper pulp by using biological enzyme |
| CN103061181A (en) * | 2012-12-31 | 2013-04-24 | 黄河三角洲京博化工研究院有限公司 | Complex enzyme preparation and technique for preparing dissolving pulp by using same |
| CN103821022A (en) * | 2014-03-11 | 2014-05-28 | 山东尤特尔生物科技有限公司 | Needle pulp and broad leaf pulp pretreatment method with neutral cellulase |
| CN104213448A (en) * | 2014-08-28 | 2014-12-17 | 华南理工大学 | Laccase/glutamic acid-based biological treatment method for inhibiting strength degradation of regenerated fibers |
| CN107254794A (en) * | 2017-06-27 | 2017-10-17 | 安徽比伦生活用纸有限公司 | A kind of method of biology enzyme auxiliary mashing |
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2017
- 2017-10-31 CN CN201711048476.2A patent/CN107653726A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010018956A1 (en) * | 1996-08-13 | 2001-09-06 | Consortium Fur Elektrochemische Industrie Gmbh | Multi-component system for modifying, degrading or bleaching lignin, lignin-containing materials or similar substances and processes for its use |
| CN101864688A (en) * | 2010-05-27 | 2010-10-20 | 岳阳纸业股份有限公司 | Pulping method of sulphate chemical pulp |
| CN102978986A (en) * | 2012-11-27 | 2013-03-20 | 天津智展科技有限公司 | Method for preparing paper pulp by using biological enzyme |
| CN103061181A (en) * | 2012-12-31 | 2013-04-24 | 黄河三角洲京博化工研究院有限公司 | Complex enzyme preparation and technique for preparing dissolving pulp by using same |
| CN103821022A (en) * | 2014-03-11 | 2014-05-28 | 山东尤特尔生物科技有限公司 | Needle pulp and broad leaf pulp pretreatment method with neutral cellulase |
| CN104213448A (en) * | 2014-08-28 | 2014-12-17 | 华南理工大学 | Laccase/glutamic acid-based biological treatment method for inhibiting strength degradation of regenerated fibers |
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| 张全升 等: ""两种漆酶改性方法及其在造纸纤维改性中的应用"", 《造纸科学与技术》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111424448A (en) * | 2020-03-30 | 2020-07-17 | 长沙瑞福尼新材料科技有限公司 | Preparation process of leaf biomechanical pulp |
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Application publication date: 20180202 |