CN107647163A - A kind of absorption spice immunization method of coccidial vaccine - Google Patents
A kind of absorption spice immunization method of coccidial vaccine Download PDFInfo
- Publication number
- CN107647163A CN107647163A CN201710825901.8A CN201710825901A CN107647163A CN 107647163 A CN107647163 A CN 107647163A CN 201710825901 A CN201710825901 A CN 201710825901A CN 107647163 A CN107647163 A CN 107647163A
- Authority
- CN
- China
- Prior art keywords
- spice
- vaccine
- adsorbent
- coccidial
- absorption
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229960005486 vaccine Drugs 0.000 title claims abstract description 133
- 235000013599 spices Nutrition 0.000 title claims abstract description 129
- 238000000034 method Methods 0.000 title claims abstract description 52
- 238000010521 absorption reaction Methods 0.000 title claims abstract description 39
- 238000002649 immunization Methods 0.000 title claims abstract description 34
- 230000003053 immunization Effects 0.000 title claims abstract description 30
- 241000287828 Gallus gallus Species 0.000 claims abstract description 56
- 239000003463 adsorbent Substances 0.000 claims abstract description 55
- 239000000203 mixture Substances 0.000 claims description 46
- 239000007788 liquid Substances 0.000 claims description 43
- 238000003756 stirring Methods 0.000 claims description 39
- 239000000463 material Substances 0.000 claims description 20
- 239000003085 diluting agent Substances 0.000 claims description 18
- 239000004459 forage Substances 0.000 claims description 17
- 235000020188 drinking water Nutrition 0.000 claims description 16
- 239000003651 drinking water Substances 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 8
- 239000002023 wood Substances 0.000 claims description 7
- 210000001508 eye Anatomy 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 239000012895 dilution Substances 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 3
- 239000011707 mineral Substances 0.000 claims description 3
- 239000000375 suspending agent Substances 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 3
- 239000000576 food coloring agent Substances 0.000 claims description 2
- 239000002775 capsule Substances 0.000 abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 7
- 239000002594 sorbent Substances 0.000 abstract description 2
- 235000013330 chicken meat Nutrition 0.000 description 50
- 235000013601 eggs Nutrition 0.000 description 29
- 238000012360 testing method Methods 0.000 description 15
- 230000005847 immunogenicity Effects 0.000 description 10
- 230000029142 excretion Effects 0.000 description 8
- 241000224483 Coccidia Species 0.000 description 7
- 241000252983 Caecum Species 0.000 description 6
- 210000004534 cecum Anatomy 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 210000003250 oocyst Anatomy 0.000 description 6
- 208000003495 Coccidiosis Diseases 0.000 description 5
- 206010023076 Isosporiasis Diseases 0.000 description 5
- 230000001900 immune effect Effects 0.000 description 5
- 210000000813 small intestine Anatomy 0.000 description 5
- 230000037213 diet Effects 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000003340 mental effect Effects 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 230000002265 prevention Effects 0.000 description 3
- 241000271566 Aves Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- OEYQBKYISMRWQB-UHFFFAOYSA-N Santal Chemical compound C=1C(OC)=CC(O)=C(C2=O)C=1OC=C2C1=CC=C(O)C(O)=C1 OEYQBKYISMRWQB-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 230000016087 ovulation Effects 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001165 anti-coccidial effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 235000012730 carminic acid Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003224 coccidiostatic agent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/012—Coccidia antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
- A61K2039/552—Veterinary vaccine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Environmental Sciences (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention discloses a kind of absorption spice immunization method of coccidial vaccine, by first by coccidial vaccine diluted, again with edibility, space is big, water absorbing capacity is strong and easily dispersible adsorbent uniformly mixes, egg capsule is set effectively evenly to adsorb on the sorbent, a possible life condition is provided for coccidial vaccine, the adsorbent of attachment egg capsule is immunized after being stirred with feed for chicken feed, can solve the problems, such as that lack of homogeneity is immunized in spice well, this method is simple to operate, to chicken group stress be small, labor intensity is low, it is adapted to be widely used in being immunized for coccidial vaccine.
Description
Technical field
The present invention relates to the immunological technique field of animal, the absorption spice immunization method of more particularly to a kind of coccidial vaccine.
Background technology
Chicken coccidiasis be threaten poultry husbandry development a kind of important diseases, the chick within its monthly age of main harm 3, to support
The harm of Growing Chicken is extremely serious, and the death rate is high, and chicken of recovering is slow-growing, depauperation, increases weight, laying eggs is severely impacted,
And easily cause the generation of Other diseases, very big economic loss is brought to poultry husbandry, has become the weight for hindering chicken farm development
Want one of disease.
The prevention of chicken coccidiasis at present is still based on coccidiostat, however, using anticoccidial with lack of standardization for a long time
Medicine, coccidia produce drug resistance and increasingly protruded the problems such as food medicament residue, and new drug development is made slow progress.Has chicken at present
Coccidiosis vaccine succeeds in developing listing, is received by the market because its application method is quick and protecting effect is excellent.With green
The arrival of animal husbandry, it is trend of the times to prevent chicken coccidiasis using chicken coccidiasis vaccine immunity.Vaccine immunity method includes spray plumage
Hair, the injection of eye drip eyeball, embryo egg, mix（Spray）The methods of material, mixed drink and drip.Wherein the immune and mixed drink of spice is immune has operation letter
It is single, to chicken group stress the small, advantage such as labor intensity is low, and spray feather immunization and waste vaccine and need particular spray equipment, drip
The immunization method of eyes, embryo egg injection and drip need to be carried out by plumage, and labor intensity is big, and cost of labor is high.It is however, only mixed at present
Drink immunization method is widely used in Immune Prevention of Avian Coccidiosis, and spice is immune to have the problem of immune lack of homogeneity, is on the one hand
Because vaccine is the aqueous solution, it is adsorbed at once when being sprayed onto feed, without mobility, stirs and be not easy；On the other hand by
Its particle proterties is maintained in feed, moisture is extremely low, and the survival of coccidian oocyst needs suitable humidity, if attached for a long time
On the feed of water shortage, easily cause vaccine fractions inactivation, it is difficult to ensure immune effect.Therefore, spice is immune not yet obtains
Popularization and application well.
It can be seen that prior art could be improved and improve.
The content of the invention
In view of in place of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of absorption spice of coccidial vaccine
Immunization method, it is intended to solve the immune lack of homogeneity that spice is immune in Immune Prevention of Avian Coccidiosis in the prior art, cannot be wide
The problem of general application.
In order to achieve the above object, this invention takes following technical scheme：
A kind of absorption spice immunization method of coccidial vaccine, comprises the following steps：
A. weighing：According to the quantity of immune plumage chicken, according to feed: adsorbent: vaccine diluent=（10~40）∶（1~4）∶（1.5
~6）Weight compare weighing；
B. the dilution of vaccine：The coccidial vaccine of 1000 plumage parts/bottle is taken, 1 bottle of vaccine is diluted with 0.2~0.6L vaccine diluent,
Pour into after the coccidial vaccine is shaken up in step A in load weighted vaccine diluent, be stirred until homogeneous repeatedly；
C. batch mixing：The vaccine liquid diluted in step B is poured slowly into equipped with the bucket of load weighted adsorbent in step A, one
Material in the agitator of falling one side of side, until adsorbent is mixed thoroughly with vaccine liquid；
D. spice：Vaccine liquid in the feed weighed in step A and step C and the mixture of adsorbent are stirred, until
Naked eyes have no agglomerate；
E. it is immunized：The forage mixture being well mixed in step D is fed into chicken group.
The absorption spice immunization method of described coccidial vaccine, wherein, adsorbent in described step A include wood powder,
Sawdust powder, wood chip, stalk, corncob.
The absorption spice immunization method of described coccidial vaccine, wherein, food is also included in the adsorbent in described step A
With the one or more of pigment, vitamin, suspending agent or mineral matter.
The absorption spice immunization method of described coccidial vaccine, wherein, the vaccine diluent in described step A is to drink
Water.
The absorption spice immunization method of described coccidial vaccine, wherein, spice in described step D for manual spice or
Mechanical spice.
The absorption spice immunization method of described coccidial vaccine, wherein, described manual spice will weigh in step A
The mixture of vaccine liquid and adsorbent in feed and step C is free-hand or is stirred repeatedly with scoop and stirring to the visually group of having no
Block.
The absorption spice immunization method of described coccidial vaccine, wherein, described mechanical spice will weigh in step A
The mixture of vaccine liquid and adsorbent in feed and step C is poured into mixer, is stirred to visually having no agglomerate.
The absorption spice immunization method of described coccidial vaccine, wherein, described step E is that will be well mixed in step D
Forage mixture dispensed according to every 100~300 plumage chicken, one charging tray or hopper, place hen house and feed chicken group.
Beneficial effect：
The invention provides a kind of absorption spice immunization method of coccidial vaccine, by that will have, edibility, space be big, water absorbing capacity
Power is strong and easily dispersible adsorbent mixes with coccidial vaccine, egg capsule is effectively adsorbed on adsorbent, adheres to the suction of egg capsule
Attached dose stirred with feed after be immunized for chicken feed, can solve the problems, such as that lack of homogeneity is immunized in spice well, should
Method is simple to operate, to chicken group stress be small, labor intensity is low, be adapted to be widely used in coccidial vaccine it is immune on.
Brief description of the drawings
Fig. 1 is a kind of flow chart of the absorption spice immunization method of coccidial vaccine provided by the invention.
Embodiment
The present invention provides a kind of absorption spice immunization method of coccidial vaccine, for make the purpose of the present invention, technical scheme and
Effect is clearer, clear and definite, and the present invention is described in more detail for the embodiment that develops simultaneously referring to the drawings.It should be appreciated that herein
Described specific embodiment only to explain the present invention, is not intended to limit the present invention.
Referring to Fig. 1, the present invention provides a kind of absorption spice immunization method of coccidial vaccine, comprise the following steps：
A. weighing：According to the quantity of immune plumage chicken, according to feed: adsorbent: vaccine diluent=（10~40）∶（1~4）∶（1.5
~6）Weight compare weighing；
B. the dilution of vaccine：The coccidial vaccine of 1000 plumage parts/bottle is taken, 1 bottle of vaccine is diluted with 0.2~0.6L vaccine diluent,
Pour into after the coccidial vaccine is shaken up in step A in load weighted vaccine diluent, be stirred until homogeneous repeatedly；
C. batch mixing：The vaccine liquid diluted in step B is poured slowly into equipped with the bucket of load weighted adsorbent in step A, one
Material in the agitator of falling one side of side, until adsorbent is mixed thoroughly with vaccine liquid；Vaccine diluent before mixing is necessary
Stir, avoid egg capsule from being precipitated in vaccine diluent and cause to mix with adsorbent uneven, influence immune uniformity；
D. spice：Vaccine liquid in the feed weighed in step A and step C and the mixture of adsorbent are stirred, until
Naked eyes have no agglomerate；
E. it is immunized：The forage mixture being well mixed in step D is fed into chicken group；Stage before immune, chicken normally feed
Food and drinking-water, control food control water is forbidden to influence immune effect.
Using above-mentioned absorption spice immunization method, by first by the absorption of vaccine liquid on the sorbent, then by vaccine liquid and
The mixture of adsorbent carries out immune nursing after being well mixed with feed；Efficiently solve spice and be immunized and be difficult to reach uniform
The problem of immune.
Specifically, the adsorbent in described step A includes wood powder, sawdust powder, wood chip, stalk, corncob；The wood powder
Include the powder of the timber such as pine, poplar, santal, in the present embodiment, using the one or more in above-mentioned adsorbent, and it is unlimited
Listed enumerated in above-mentioned；Adsorbent employed in the present embodiment is respectively provided with edibility, space is big, water absorbing capacity is strong, can
Quickly and evenly adsorbed vaccine liquid, and certain wettability is kept, it ensure that the life condition of coccidian oocyst, and the absorption
Agent easily disperses, and can be well dispersed in feed, ensures immune uniformity.
Further, food coloring, vitamin, suspending agent or mineral matter are also included in the adsorbent in described step A
One or more；A small amount of famille rose is preferably added in the present embodiment as indicator, can preferably reflect adsorbent
The uniformity mixed with feed, and carmine appropriate addition is to meet the requirement of food hygienic standard, will not cause food source
Contact scar.
Specifically, the vaccine diluent in described step A is drinking water；Bacterial content in drinking water is fewer, prevents
Adverse effect of the microorganism to immune effect in sealing.
Further, the spice in described step D is manual spice or mechanical spice；Manual spice is easy to implement, no
Any apparatus is needed, is adapted to small-scale cultivation demand；Mechanical spice efficiency high, the time is saved, uniformity is good, is adapted to large-scale agriculture
The demand of field；In implementation process, different spice modes can be selected according to demand.
Specifically, described manual spice is the vaccine liquid and adsorbent in the feed and step C that will be weighed in step A
Mixture is stirred single-handedly or repeatedly with stirring to visually having no agglomerate with scoop.
Specifically, described mechanical spice is the vaccine liquid and adsorbent in the feed and step C that will be weighed in step A
Mixture is poured into mixer, is stirred to visually having no agglomerate.
Preferably, described step E is the forage mixture that will be well mixed in step D according to every 100~300 plumage chicken one
Individual charging tray or hopper packing, places hen house and feeds chicken group；It is again not unrestrained while defined feed intake to ensure that chicken group can obtain
Take and feed space；Preferably per plumage chicken feeding 1.25g feed and vaccine liquid, the mixture of adsorbent, this feeding in the present embodiment
Amount has reached best immune effect.
The present invention specific experiment process be：
（1）Experiment packet and processing
3 age in days Huang plumage chickens 60 are taken, are randomly divided into 3 groups, every group 20, it is spice immune group to set the 1st group（Manual spice）, the
2 groups are absorption spice immune group（Manual spice）, the 3rd group is absorption spice immune group（Mechanical spice）, each group chicken is in 3 ages in days point
Do not feed the above-mentioned other feed of each group to stir to be immunized, 1.25g/, 1 plumage part/only, immune ingest of each group is taken
Between be respectively spice immune group（Manual spice）0.5 hour, adsorb spice immune group（Manual spice）1 hour, absorption spice was exempted from
Epidemic disease group（Mechanical spice）1 hour, each group was respectively adopted on cage and raised, and is specifically shown in Table 1.
The packet and processing that table 1 is tested
（2）Experimental observation project
Immune uniformity：The random excrement for taking above-mentioned every group 6~7 days 5 chicken after immune respectively, individually detect the small of every chicken
The egg sac number of intestines excrement and caecum excrement, count the recall rate of egg capsule.
Egg capsule OPG values（Number of the every gram of excrement containing egg capsule）Calculating：Every group takes caecum excrement and small intestine excrement, by 1:4 ratio
After example mixing, with the egg capsule OPG values of Maxwell tally method meter excrement.
Coccidial vaccine used in examples provided below is selected from Zhengdian Biological Tech. Co., Ltd., Foshan City
Coccidiosis trivalent live vaccine（1000 plumage parts/bottle）, credit number：（2016）Veterinary drug production card word 19046；It is worth explanation
It is all vaccines that the solution of the present invention is applied to preventing and treating global-worm illness, it is not limited to described used in the embodiment of the present invention
Coccidiosis trivalent live vaccine.
Embodiment 1
Formed according to method of mixing material and the different of forage mixture, point three groups of test process, three test groups are：
1. spice immune group（Manual spice）：Take 10 bottles of coccidial vaccines to be diluted in 1L drinking water, delay after stirring
Slowly be poured into 11.5kg feed, lean to one side into coccidia vaccine diluent while stir forage mixture, stir repeatedly and
Stirring, mix to visually having no agglomerate.
2. adsorb spice immune group（Manual spice）：10 bottles of coccidial vaccines are taken to be diluted in 2L drinking water, stirring is equal
Even, the vaccine liquid diluted is poured slowly into the bucket equipped with 1kg adsorbents, the material in one side agitator that leans to one side, until inhaling
Attached dose is mixed thoroughly with vaccine liquid, then weigh 9.5kg feed and the mixture of vaccine liquid and adsorbent with scoop repeatedly
Stir with stirring to visually having no agglomerate.
3. adsorb spice immune group（Mechanical spice）：10 bottles of coccidial vaccines are taken to be diluted in 2L drinking water, stirring is equal
Even, the vaccine liquid diluted is poured slowly into the bucket equipped with 1kg adsorbents, the material in one side agitator that leans to one side, until inhaling
Attached dose is mixed thoroughly with vaccine liquid, then weighs 9.5kg feed and the mixture of vaccine liquid and adsorbent pours into mixer
In, stir to visually having no agglomerate.
The forage mixture of above-mentioned three groups of method of mixing material is fed into chicken, chicken mental status is good during whole experiment
Good, diet is normal, and 6~7 days immunogenicities for detecting each group chicken respectively after being immunized, testing result is as shown in 2~table of table 8.
The spice immune group of table 2（Manual spice）The immunogenicity testing result of the 6th day after immune
Note：OPG values are that Maxwell tally egg capsule reading is multiplied by 100 under microscope.
Table 3 adsorbs spice immune group（Manual spice）The immunogenicity testing result of the 6th day after immune
Table 4 adsorbs spice immune group（Mechanical spice）The immunogenicity testing result of the 6th day after immune
The spice immune group of table 5（Manual spice）The immunogenicity testing result of the 7th day after immune
Table 6 adsorbs spice immune group（Manual spice）The immunogenicity testing result of the 7th day after immune
Table 7 adsorbs spice immune group（Mechanical spice）The immunogenicity testing result of the 7th day after immune
The each group egg capsule OPG values of table 8 and recall rate
From 2~table of table 8, spice immune group（Manual spice）, absorption spice immune group（Manual spice）, absorption spice be immunized
Group（Mechanical spice）The small intestine excrement of chicken and the egg capsule recall rate of caecum excrement are 100% in three groups, and the egg capsule amount discharged all accords with
Oocysts Excretion Regulation and number range after conjunction first immunisation, in terms of above-mentioned each worm kind egg capsule detection data, 6 days after being immunized are huge
Ovulation capsule peak period, be immunized after 6~7 days be tender ovulation capsule peak period, this meets the circulation law of vaccine egg capsule.But
Because chicken individuals have differences, the data of shown single chicken also have corresponding difference.Adsorb spice immune group（Manual spice）
With absorption spice immune group（Mechanical spice）Mixing excrement sample egg capsule excretion is suitable, and the immune immune uniformity of absorption spice is good
It is good, meet the application of chicken coccidiasis live vaccine.
Embodiment 2
Formed according to method of mixing material and the different of forage mixture, point three groups of test process, three test groups are：
1. spice immune group（Manual spice）：Take 10 bottles of coccidial vaccines to be diluted in 2L drinking water, delay after stirring
Slowly it is poured into 32kg feed, leans to one side into coccidia vaccine diluent while stir forage mixture, stir and stir repeatedly
Mix, mix to visually having no agglomerate.
2. adsorb spice immune group（Manual spice）：10 bottles of coccidial vaccines are taken to be diluted in 4L drinking water, stirring is equal
Even, the vaccine liquid diluted is poured slowly into the bucket equipped with 2kg adsorbents, the material in one side agitator that leans to one side, until inhaling
Attached dose is mixed thoroughly with vaccine liquid, then weighs 28kg feed and the mixture of vaccine liquid and adsorbent is turned over repeatedly with scoop
It is dynamic with stirring to visually having no agglomerate.
3. adsorb spice immune group（Mechanical spice）：10 bottles of coccidial vaccines are taken to be diluted in 4L drinking water, stirring is equal
Even, the vaccine liquid diluted is poured slowly into the bucket equipped with 2kg adsorbents, the material in one side agitator that leans to one side, until inhaling
Attached dose is mixed thoroughly with vaccine liquid, then weighs 28kg feed and the mixture of vaccine liquid and adsorbent pours into mixer
In, mix to visually having no agglomerate.
The forage mixture of above-mentioned three groups of method of mixing material is fed into chicken, chicken mental status is good during whole experiment
Good, diet is normal, 6~7 days immunogenicities for detecting each group chicken respectively after being immunized, the small intestine excrement of chicken and caecum excrement in three groups
Egg capsule recall rate is 100%, and the egg capsule amount discharged all meets Oocysts Excretion Regulation and number range after first immunisation, is adsorbed
Spice immune group（Manual spice）With absorption spice immune group（Mechanical spice）It is suitable to mix excrement sample egg capsule excretion, adsorbs spice
The immune immune application for having good uniformity, meeting chicken coccidiasis live vaccine.
Embodiment 3
Formed according to method of mixing material and the different of forage mixture, point three groups of test process, three test groups are：
1. spice immune group（Manual spice）：10 bottles of coccidial vaccines are taken to be diluted in 2.3L drinking water, after stirring
Slowly be poured into 38kg feed, lean to one side into coccidia vaccine diluent while stir forage mixture, stir repeatedly and
Stirring, mix to visually having no agglomerate.
2. adsorb spice immune group（Manual spice）：Take 10 bottles of coccidial vaccines to be diluted in 2.5L drinking water, stir
Uniformly, the vaccine liquid diluted is poured slowly into the bucket equipped with 2.8kg adsorbents, the material in one side agitator that leans to one side, directly
Be mixed thoroughly to adsorbent and vaccine liquid, then weigh 35kg feed and the mixture scoop of vaccine liquid and adsorbent it is anti-
Stir again with stirring to visually having no agglomerate.
3. adsorb spice immune group（Mechanical spice）：Take 10 bottles of coccidial vaccines to be diluted in 2.5L drinking water, stir
Uniformly, the vaccine liquid diluted is poured slowly into the bucket equipped with 2.8kg adsorbents, the material in one side agitator that leans to one side, directly
It is mixed thoroughly to adsorbent and vaccine liquid, then weighs 35kg feed and the mixture of vaccine liquid and adsorbent pours into stirring
In machine, mix to visually having no agglomerate.
The forage mixture of above-mentioned three groups of method of mixing material is fed into chicken, chicken mental status is good during whole experiment
Good, diet is normal, 6~7 days immunogenicities for detecting each group chicken respectively after being immunized, the small intestine excrement of chicken and caecum excrement in three groups
Egg capsule recall rate is 100%, and the egg capsule amount discharged all meets Oocysts Excretion Regulation and number range after first immunisation, is adsorbed
Spice immune group（Manual spice）With absorption spice immune group（Mechanical spice）It is suitable to mix excrement sample egg capsule excretion, adsorbs spice
The immune immune application for having good uniformity, meeting chicken coccidiasis live vaccine.
Embodiment 4
Formed according to method of mixing material and the different of forage mixture, point three groups of test process, three test groups are：
1. spice immune group（Manual spice）：10 bottles of coccidial vaccines are taken to be diluted in 4.5L drinking water, after stirring
Slowly it is poured into 43.5kg feed, leans to one side into coccidia vaccine diluent while stir forage mixture, stir repeatedly
And stirring, mix to visually having no agglomerate.
2. adsorb spice immune group（Manual spice）：10 bottles of coccidial vaccines are taken to be diluted in 6L drinking water, stirring is equal
Even, the vaccine liquid diluted is poured slowly into the bucket equipped with 4kg adsorbents, the material in one side agitator that leans to one side, until inhaling
Attached dose is mixed thoroughly with vaccine liquid, then weighs 39kg feed and the mixture of vaccine liquid and adsorbent is turned over repeatedly with scoop
It is dynamic with stirring to visually having no agglomerate.
3. adsorb spice immune group（Mechanical spice）：10 bottles of coccidial vaccines are taken to be diluted in 6L drinking water, stirring is equal
Even, the vaccine liquid diluted is poured slowly into the bucket equipped with 4kg adsorbents, the material in one side agitator that leans to one side, until inhaling
Attached dose is mixed thoroughly with vaccine liquid, then weighs 39kg feed and the mixture of vaccine liquid and adsorbent pours into mixer
In, mix to visually having no agglomerate.
The forage mixture of above-mentioned three groups of method of mixing material is fed into chicken, chicken mental status is good during whole experiment
Good, diet is normal, 6~7 days immunogenicities for detecting each group chicken respectively after being immunized, the small intestine excrement of chicken and caecum excrement in three groups
Egg capsule recall rate is 100%, and the egg capsule amount discharged all meets Oocysts Excretion Regulation and number range after first immunisation, is adsorbed
Spice immune group（Manual spice）With absorption spice immune group（Mechanical spice）It is suitable to mix excrement sample egg capsule excretion, adsorbs spice
The immune immune application for having good uniformity, meeting chicken coccidiasis live vaccine.
In summary, the invention provides a kind of absorption spice immunization method of coccidial vaccine, by will have edibility,
Space is big, water absorbing capacity is strong and easily dispersible adsorbent mixes with coccidial vaccine, egg capsule is effectively adsorbed on adsorbent,
The adsorbent of attachment egg capsule is immunized after being stirred with feed for chicken feed, can solve spice well and uniformity is immunized
The problem of poor, this method is simple to operate, to chicken group stress be small, labor intensity is low, is adapted to be widely used in coccidial vaccine
On immune.
It is understood that for those of ordinary skills, can be with technique according to the invention scheme and its hair
Bright design is subject to equivalent substitution or change, and all these changes or replacement should all belong to the guarantor of appended claims of the invention
Protect scope.
Claims (8)
1. the absorption spice immunization method of a kind of coccidial vaccine, it is characterised in that comprise the following steps：
A. weighing：According to the quantity of immune plumage chicken, according to feed: adsorbent: vaccine diluent=（10~40）∶（1~4）∶（1.5
~6）Weight compare weighing；
B. the dilution of vaccine：The coccidial vaccine of 1000 plumage parts/bottle is taken, 1 bottle of vaccine is diluted with 0.2~0.6L vaccine diluent,
Pour into after the coccidial vaccine is shaken up in step A in load weighted vaccine diluent, be stirred until homogeneous repeatedly；
C. batch mixing：The vaccine liquid diluted in step B is poured slowly into equipped with the bucket of load weighted adsorbent in step A, one
Material in the agitator of falling one side of side, until adsorbent is mixed thoroughly with vaccine liquid；
D. spice：Vaccine liquid in the feed weighed in step A and step C and the mixture of adsorbent are stirred, until
Naked eyes have no agglomerate；
E. it is immunized：The forage mixture being well mixed in step D is fed into chicken group.
2. the absorption spice immunization method of coccidial vaccine according to claim 1, it is characterised in that in described step A
Adsorbent include wood powder, sawdust powder, wood chip, stalk, corncob.
3. the absorption spice immunization method of coccidial vaccine according to claim 1, it is characterised in that in described step A
Adsorbent in also include the one or more of food coloring, vitamin, suspending agent or mineral matter.
4. the absorption spice immunization method of coccidial vaccine according to claim 1, it is characterised in that in described step A
Vaccine diluent be drinking water.
5. the absorption spice immunization method of coccidial vaccine according to claim 1, it is characterised in that in described step D
Spice be manual spice or mechanical spice.
6. the absorption spice immunization method of coccidial vaccine according to claim 5, it is characterised in that described manual spice
For the vaccine liquid in the feed weighed in step A and step C and the mixture of adsorbent are stirred single-handedly or with scoop repeatedly with
Stirring is to visually having no agglomerate.
7. the absorption spice immunization method of coccidial vaccine according to claim 5, it is characterised in that described mechanical spice
For the vaccine liquid in the feed weighed in step A and step C and the mixture of adsorbent are poured into mixer, stir to naked eyes
Have no agglomerate.
8. the absorption spice immunization method of coccidial vaccine according to claim 1, it is characterised in that described step E is
The forage mixture being well mixed in step D is dispensed according to every 100~300 plumage chicken, one charging tray or hopper, hen house is placed and throws
Feed chicken group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710825901.8A CN107647163A (en) | 2017-09-14 | 2017-09-14 | A kind of absorption spice immunization method of coccidial vaccine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710825901.8A CN107647163A (en) | 2017-09-14 | 2017-09-14 | A kind of absorption spice immunization method of coccidial vaccine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107647163A true CN107647163A (en) | 2018-02-02 |
Family
ID=61130535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710825901.8A Pending CN107647163A (en) | 2017-09-14 | 2017-09-14 | A kind of absorption spice immunization method of coccidial vaccine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107647163A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4752475A (en) * | 1977-11-14 | 1988-06-21 | Unilever Patent Holdings B.V. | Compositions for the control of coccidiosis in poultry |
| CN106798924A (en) * | 2017-03-09 | 2017-06-06 | 佛山市正典生物技术有限公司 | String is preparing the application of prevention chicken coccidiasis medicine |
-
2017
- 2017-09-14 CN CN201710825901.8A patent/CN107647163A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4752475A (en) * | 1977-11-14 | 1988-06-21 | Unilever Patent Holdings B.V. | Compositions for the control of coccidiosis in poultry |
| CN106798924A (en) * | 2017-03-09 | 2017-06-06 | 佛山市正典生物技术有限公司 | String is preparing the application of prevention chicken coccidiasis medicine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102481371B (en) | Sticky soft gel for treating poultry | |
| CN102908615B (en) | Novel haemophilus parasuis disease trivalent inactivated vaccine and preparation method thereof | |
| CN104521885A (en) | Antibiotic-free broiler chicken feeding method | |
| CN103814967A (en) | Chinese herbal medicine granular preparation used for aquatic product disinfection and preparing method thereof | |
| CN111579320A (en) | Egg standard substance containing chloramphenicol, preparation method and application | |
| Tensa et al. | Comparison of the application parameters of coccidia vaccines by gel and spray | |
| CN104645337A (en) | Coccidial vaccine suspending aid | |
| CN102145011B (en) | Composition for preventing and curing diarrhea, preparation method and application of composition | |
| CN109123100A (en) | A kind of microcapsule feedstuff additive and preparation method thereof and animal and fowl fodder | |
| CN109287885A (en) | For improving the additive and its preparation method and application of growth of animal performance | |
| CN107647163A (en) | A kind of absorption spice immunization method of coccidial vaccine | |
| CN104906179A (en) | Origanum oil suspension and preparation method thereof | |
| CN102389036B (en) | Macroergic multivitamin-amino acid emulsion and preparation method thereof | |
| CN103609874A (en) | Animal feed additive, and premix and feed thereof | |
| CN106798924A (en) | String is preparing the application of prevention chicken coccidiasis medicine | |
| Shuford et al. | Antimicrobial growth promoter use in livestock-implications for human health | |
| CN106420622B (en) | A kind of water-soluble preparation of Tilmicosin Ultramicro-powder and preparation method thereof | |
| CN107897507A (en) | A kind of herb fermenting composition and its application in field of animal feed | |
| CN104222625A (en) | Feed for weaned piglet and application of feed | |
| CN104739847A (en) | Compound vitamin oral solution composition and preparation method thereof | |
| CN103844001B (en) | One compound premix for breeding swine | |
| CN109566896A (en) | One kind having anti-stress effect poultry general nano nutritional agents and preparation method thereof | |
| EP2968159A1 (en) | Managing osteoporosis with hmw peg | |
| CN103599103B (en) | Hainanmycin sodium solution and preparation method thereof | |
| EP4461141A2 (en) | Powder composition and gel composition comprising aquatic photosynthesizing organisms |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180202 |