CN107646573A - A kind of biological control method of powdery mildew of strawberry - Google Patents
A kind of biological control method of powdery mildew of strawberry Download PDFInfo
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- CN107646573A CN107646573A CN201710806898.5A CN201710806898A CN107646573A CN 107646573 A CN107646573 A CN 107646573A CN 201710806898 A CN201710806898 A CN 201710806898A CN 107646573 A CN107646573 A CN 107646573A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protection of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- Dentistry (AREA)
- Microbiology (AREA)
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- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
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- Virology (AREA)
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Abstract
The invention discloses a kind of biological control method of powdery mildew of strawberry, is related to arable farming and disease prevention techniques field；Described prevention and controls are：With microbiological treatment soil before strawberry cultivating and in planting process, spray on plants source antiseptic is prevented and treated；Prevented and treated using the method for the present invention, prevention effect is good, quick, and take effect cycle length, and pathogenic bacteria drug resistance is poor, no recurrent exerbation phenomenon.
Description
【Technical field】
The present invention relates to arable farming and disease prevention techniques field, and in particular to a kind of biological control of powdery mildew of strawberry
Method.
【Background technology】
Powdery mildew of strawberry is caused by the single softgel shell bacterium of garment or robe made of feathers grass of fungi Ascomycotina Sphaerotheca is infected, and is strawberry kind
One of main disease during plant.Easily fallen ill under conditions of low temperature and high relative humidity, most suitable onset temperature is 15-25 DEG C, relatively
Humidity is more than 90%；Main harm leaf, petiole, flower, anthocaulus and fruit, morbidity cardinal symptom are：Early stage is in morbidity portion
Bit length goes out very thin white hypha layer, and subsequent scab gradually expands, and produces white powder thing, the later stage is in bronzing scab, is caused
Blade upsweeps into soupspoon shape, leaf margin atrophy, shrivelled, and bud does not open, and fruit dries up, and color and luster is deteriorated；It is sick when falling ill serious
For leaf rate more than 45%, diseased fruit rate has had a strong impact on the yield and quality of strawberry more than 50%.
The major transmission path of powdery mildew is：Survive the winter and get in diseased plant or in invalid body with mycelium or conidium
In the summer, turn into the source of infection in the coming year, mainly carry out long distance by media such as wind-force, animals by the Strawberry Seedlings or conidium that carry disease germs
From propagation.
Current main prevention and controls have：Reasonable adjusting implantation methods, cleaning old leaf diseased plant, eliminate bacterium source；With how clever bacterium or
Chemical pesticide controls such as wettable powder etc..But cleaning elimination cause of disease can not thoroughly remove the germ in soil, chemical pesticide
It is high to prevent and treat toxicity, it is quick, but germ drug resistance is strong, and easy recurrent exerbation, the cycle that takes effect is short, plus disease mainly by mitogenetic
Spore is propagated by media such as wind or animals, and spread speed is fast, and area is wide, so it is difficult to prevent the diffusion of disease, is controlled
Rate is low.
【The content of the invention】
In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide a kind of biological control side of powdery mildew of strawberry
Method, this method are resisted according to powdery mildew characteristics of incidence by microbiological treatment soil before plantation and spray on plants source in planting process
Microbial inoculum preventing and treating is combined, and makes controlling powdery mildew effect good, quick, and take effect cycle length, and pathogenic bacteria drug resistance is poor, no recurrent exerbation
Phenomenon.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
A kind of biological control method of powdery mildew of strawberry, comprises the following steps：
(1) first by Soil tillage before strawberry cultivating, bedding a layer thickness is 2-4cm moistening rice on soil after turning over
Grass, after bedding terminates, the complex microorganism matrix of 10-20kg/ mus is uniformly sprinkling upon on straw, and is covered in antibacterial matrix
A layer thickness is 1-3cm fine earth, watering, overlay film, in coating process every 6-8h to membrane gas treatment once, after 4-6 days
Remove film plantation strawberry；The complex microorganism matrix is mainly mixed by the raw material of following parts by weight：Edible fungi residues
20-23 parts, Pseudomonas aeruginosa 3-5 parts, bacillus subtilis 6-8 parts, streptomycete 1-3 parts, Paecilomyces lilacinus 2-4 parts and saccharomycete
9-11 parts；
(2) strawberry of each growth phase is sprayed with plant-source antibacterial agent；Seedling Stage sprays once, each amount of application
For 20-50mL/ mus；Vigorous period was applied once every 5-7 days, and each amount of application is 90-100mL/ mus；Florescence is every 3-
Apply once within 5 days, each amount of application is 70-90mL/ mus；The Chinese olive phase applied once every 2-4 days, and each amount of application is 65-
75mL/ mus；The haw phase applied once every 2-3 days, and each amount of application is 50-65mL/ mus, you can effectively prevents and treats powdery mildew；
The plant-source antibacterial agent is mainly mixed by the raw material of following parts by weight：Purple common perilla seed 10-15 parts,
Cumin seed extract 8-11 parts, fulvic acid 4-6 parts and aqueous emulsion 6-9 parts；
The preparation method of the purple common perilla seed is：Fresh purple perilla seed is dried to constant weight, grinds at 80-85 DEG C
End, after powder is mixed with the water of 2-3 times of weight, added into compound the powder weight 0.2-0.3% cellulase,
0.1-0.2% pectase and 0.15-0.25% protease mix, and adjustment pH is 4-5, and 2-3h, mistake are reacted at 45-55 DEG C
Filter, filtrate is centrifuged, takes supernatant liquor, produces the purple common perilla seed；
The preparation method of the cumin seed extract is：Fresh cumin seed is dried to constant weight by A at 70-75 DEG C,
And clay into power, after the powder and water mixed in equal amounts, the microwave treatment 30-60s under 800-1000W is standby；B is to microwave
The ethanol solution that 5-7 times of weight is added in compound after processing mixes, and refluxing extraction 2-3 times at 90-100 DEG C, returns every time
1-2h is flowed, merges ethanol extract, produces the cumin seed extract.
Further, in step (1), the water content of the moistening straw is 50-60%.
Preferably, in step (1), the complex microorganism matrix is mainly mixed by the raw material of following parts by weight：
22 parts of edible fungi residues, 4 parts of Pseudomonas aeruginosa, 7 parts of bacillus subtilis, 2 parts of streptomycete, 3 parts of Paecilomyces lilacinus and saccharomycete 10
Part.
Preferably, in step (2), the plant-source antibacterial agent is mainly mixed by the raw material of following parts by weight：It is purple
7 parts of 13 parts of Perillaseed extract, 10 parts of cumin seed extract, 5 parts of fulvic acid and aqueous emulsion.
Further, in step (2), the plant-source antibacterial agent is diluted before applying every time with 100-200 times of water.
Further, the centrifugal rotational speed is 1000-1200r/min, centrifugation time 20-25min.
Compared with prior art, the present invention has the advantages that：
Powdery mildew of strawberry prevention and controls of the present invention according to powdery mildew characteristics of incidence, by microbiological treatment soil before plantation with
Antiseptic preventing and treating in spray on plants source is combined in planting process, makes prevention effect good, quick, and take effect cycle length, and pathogenic bacteria are resistance to
The property of medicine is poor, no recurrent exerbation phenomenon.
Complex microorganism matrix treatments soil is used in the present invention before strawberry cultivating, can effectively press down the Powdery Mildew killed in soil
Body and conidium, most of pathogeny is eliminated；Wherein, complex microorganism matrix is by edible fungi residues, Pseudomonas aeruginosa, withered grass gemma
Bacillus, streptomycete, Paecilomyces lilacinus and saccharomycete composition, Pseudomonas aeruginosa, bacillus subtilis, streptomycete, Paecilomyces lilacinus
Bacterium and saccharomycete are bred rapidly using edible fungi residues and straw as culture medium, and competitive relation is formed with pathogenic bacteria, and as in soil
Dominant population, and produce antibacterial peptide, bacteriocin, lysozyme, antimicrobial organic acid etc. and kill pathogen mycelial growth and spore with suppression
The material of daughter development, the pathogenic bacteria in soil is killed by a large amount of suppressions, reduce infection pathogeny.
The equal spray on plants source antiseptic of each growth phase of strawberry cultivating process of the present invention is prevented and treated, wherein, plant source resists
Microbial inoculum is made up of purple common perilla seed, cumin seed extract, fulvic acid and aqueous emulsion, and purple common perilla seed and cumin seed carry
Take the flavonoids in thing, Polyphenols, aldehydes, acids and polysaccharide that there is antibacterial activity effect, have inhibitory action to powdery mildew；It is yellow
Rotten acid can suitable control crop blade face stomata openness, reduce rising；Purple common perilla seed, cumin seed extract and Huang are rotten
Sour Synergistic, strengthen drug effect, make inhibition more preferable, while together sprayed in plant surface with aqueous emulsion, formed one layer thin
Liquid cover layer, can there are adhesion, parcel and inhibitory or killing effect to the thalline and its spore for being already attached to plant surface and air, it is effectively anti-
Only wind or animal are by the further diffusion and propagation of germ and its sporinite.Preventing and treating powdery mildew is carried out using the method for the present invention,
The incidence of disease as little as 2-5%, no recurrent exerbation phenomenon, better than conventional method.
【Embodiment】
The embodiment of the present invention is described further with reference to embodiments.
Embodiment 1
A kind of biological control method of powdery mildew of strawberry of the present embodiment, comprises the following steps：
(1) first by Soil tillage before strawberry cultivating, the water content that bedding a layer thickness is 2cm on soil after turning over is
50% moistening straw, after bedding terminates, the complex microorganism matrix of 10kg/ mus is uniformly sprinkling upon on straw, and in antibacterial
Cover the fine earth that a layer thickness is 1-3cm in matrix, watering, overlay film, in coating process every 6h to membrane gas treatment once, 4 days
After can remove film plantation strawberry；The complex microorganism matrix is mainly mixed by the raw material of following parts by weight：Food
With 9 parts of 20 parts of bacteria residue, 3 parts of Pseudomonas aeruginosa, 6 parts of bacillus subtilis, 1 part of streptomycete, 2 parts of Paecilomyces lilacinus and saccharomycete；
(2) strawberry of each growth phase is sprayed with plant-source antibacterial agent；Seedling Stage sprays once, each amount of application
For 20mL/ mus；Vigorous period was applied once every 5 days, and each amount of application is 90mL/ mus；Florescence applied one every 3 days
Secondary, each amount of application is 70mL/ mus；The Chinese olive phase applied once every 2 days, and each amount of application is 65mL/ mus；The haw phase is every 2
It is applied once, and each amount of application is 50mL/ mus, you can effectively prevents and treats powdery mildew；Wherein, the plant-source antibacterial agent is applied every time
Diluted with preceding with 100 times of water；
The plant-source antibacterial agent is mainly mixed by the raw material of following parts by weight：It is 10 parts of purple common perilla seed, diligent
Right 6 parts of 8 parts of seed extract, 4 parts of fulvic acid and aqueous emulsion；
The preparation method of the purple common perilla seed is：Fresh purple perilla seed is dried to constant weight at 80 DEG C, clayed into power,
After powder is mixed with the water of 2 times of weight, cellulase, 0.1% fruit of the powder weight 0.2% are added into compound
Glue enzyme and 0.15% protease mix, adjustment pH be 4,2h is reacted at 45 DEG C, filter, by filtrate under 1000r/min from
Heart 20min, takes supernatant liquor, produces the purple common perilla seed；
The preparation method of the cumin seed extract is：Fresh cumin seed is dried to constant weight by A at 70 DEG C, and is ground
Into powder, after the powder and water mixed in equal amounts, the microwave treatment 30s under 800W is standby；B is to the mixing after microwave treatment
The ethanol solution that 5 times of weight are added in material mixes, and refluxing extraction 2 times at 90 DEG C, flow back 1h every time, merges ethanol extract,
Produce the cumin seed extract.
Embodiment 2
A kind of biological control method of powdery mildew of strawberry of the present embodiment, comprises the following steps：
(1) first by Soil tillage before strawberry cultivating, the water content that bedding a layer thickness is 4cm on soil after turning over is
60% moistening straw, after bedding terminates, the complex microorganism matrix of 20kg/ mus is uniformly sprinkling upon on straw, and in antibacterial
Cover the fine earth that a layer thickness is 1-3cm in matrix, watering, overlay film, in coating process every 8h to membrane gas treatment once, 6 days
After can remove film plantation strawberry；The complex microorganism matrix is mainly mixed by the raw material of following parts by weight：Food
With 11 parts of 23 parts of bacteria residue, 5 parts of Pseudomonas aeruginosa, 8 parts of bacillus subtilis, 3 parts of streptomycete, 4 parts of Paecilomyces lilacinus and saccharomycete；
(2) strawberry of each growth phase is sprayed with plant-source antibacterial agent；Seedling Stage sprays once, each amount of application
For 50mL/ mus；Vigorous period was applied once every 7 days, and each amount of application is 100mL/ mus；Florescence applied one every 5 days
Secondary, each amount of application is 90mL/ mus；The Chinese olive phase applied once every 4 days, and each amount of application is 75mL/ mus；The haw phase is every 3
It is applied once, and each amount of application is 65mL/ mus, you can effectively prevents and treats powdery mildew；Wherein, the plant-source antibacterial agent is applied every time
Diluted with preceding with 200 times of water；
The plant-source antibacterial agent is mainly mixed by the raw material of following parts by weight：It is 15 parts of purple common perilla seed, diligent
Right 9 parts of 11 parts of seed extract, 6 parts of fulvic acid and aqueous emulsion；
The preparation method of the purple common perilla seed is：Fresh purple perilla seed is dried to constant weight at 85 DEG C, clayed into power,
After powder is mixed with the water of 3 times of weight, cellulase, 0.2% fruit of the powder weight 0.3% are added into compound
Glue enzyme and 0.25% protease mix, adjustment pH be 5,3h is reacted at 55 DEG C, filter, by filtrate under 1200r/min from
Heart 25min, takes supernatant liquor, produces the purple common perilla seed；
The preparation method of the cumin seed extract is：Fresh cumin seed is dried to constant weight by A at 75 DEG C, and is ground
Into powder, after the powder and water mixed in equal amounts, the microwave treatment 60s under 1000W is standby；B is to the mixing after microwave treatment
The ethanol solution that 7 times of weight are added in material mixes, and refluxing extraction 3 times at 100 DEG C, flow back 2h every time, merges ethanol extract,
Produce the cumin seed extract.
Embodiment 3
A kind of biological control method of powdery mildew of strawberry of the present embodiment, comprises the following steps：
(1) first by Soil tillage before strawberry cultivating, the water content that bedding a layer thickness is 3cm on soil after turning over is
55% moistening straw, after bedding terminates, the complex microorganism matrix of 15kg/ mus is uniformly sprinkling upon on straw, and in antibacterial
Cover the fine earth that a layer thickness is 1-3cm in matrix, watering, overlay film, in coating process every 7h to membrane gas treatment once, 5 days
After can remove film plantation strawberry；The complex microorganism matrix is mainly mixed by the raw material of following parts by weight：Food
With 10 parts of 22 parts of bacteria residue, 4 parts of Pseudomonas aeruginosa, 7 parts of bacillus subtilis, 2 parts of streptomycete, 3 parts of Paecilomyces lilacinus and saccharomycete；
(2) strawberry of each growth phase is sprayed with plant-source antibacterial agent；Seedling Stage sprays once, each amount of application
For 40mL/ mus；Vigorous period was applied once every 6 days, and each amount of application is 95mL/ mus；Florescence applied one every 4 days
Secondary, each amount of application is 80mL/ mus；The Chinese olive phase applied once every 3 days, and each amount of application is 70mL/ mus；The haw phase every
Apply once within 2.5 days, each amount of application is 60mL/ mus, you can effectively prevents and treats powdery mildew；Wherein, the plant-source antibacterial agent is every
Diluted before secondary administration with 150 times of water；
The plant-source antibacterial agent is mainly mixed by the raw material of following parts by weight：It is 13 parts of purple common perilla seed, diligent
Right 7 parts of 10 parts of seed extract, 5 parts of fulvic acid and aqueous emulsion；
The preparation method of the purple common perilla seed is：Fresh purple perilla seed is dried to constant weight at 84 DEG C, clayed into power,
After powder is mixed with the water of 2.5 times of weight, added into compound the powder weight 0.25% cellulase, 0.15%
Pectase and 0.20% protease mix, adjustment pH be 4.5,2.5h is reacted at 50 DEG C, filter, by filtrate in 1100r/
22min is centrifuged under min, takes supernatant liquor, produces the purple common perilla seed；
The preparation method of the cumin seed extract is：Fresh cumin seed is dried to constant weight by A at 73 DEG C, and is ground
Into powder, after the powder and water mixed in equal amounts, the microwave treatment 50s under 900W is standby；B is to the mixing after microwave treatment
The ethanol solution that 6 times of weight are added in material mixes, and refluxing extraction 3 times at 95 DEG C, flow back 1.5h every time, merges ethanol extraction
Liquid, produce the cumin seed extract.
Prevention effect experiment of the present invention：
In order to illustrate prevention effect of the present invention, applicant has done comparative testing below：Experiment is in Guangxi planting base
Carry out, experiment be divided into the 1st group, the 2nd group, the 3rd group, control group 1, control group 2, totally 6 groups of control group 3, every group is provided with 4 weights
It is multiple, it is each to repeat to plant 100 plants of strawberries.1st group, the 2nd group, the 3rd group uses embodiment 1, embodiment 2, the side of embodiment 3 respectively
Method is prevented and treated, and control group 1 without step (1), i.e., is with unused complex microorganism matrix treatments soil, other steps before plantation
It is same as Example 3；For control group 2 in step (2), each growth phase of planting process replaces plant source with 50% how clever bacterium
Antiseptic is prevented and treated, and other steps are same as Example 3；Do not contain in plant-source antibacterial agent in the step of control group 3 (2)
Fulvic acid, only purple common perilla seed and cumin seed extract, other steps are same as Example 3.6 groups other environment
Condition is consistent.Planting process counts incidence, seeks each cell mean, the wherein incidence of disease is each group morbidity strain number and total strain number
Ratio, the results are shown in Table 1：
Table 1
It was found from the 1st group, the 2nd group, the 3rd group, preventing and treating powdery mildew, the incidence of disease as little as 2- are carried out using the method for the present invention
5%, no recurrent exerbation phenomenon, better than conventional method；It was found from the 3rd group of contrast with control group 1, in the present invention before strawberry cultivating
With complex microorganism matrix treatments soil, it can effectively press down powdery mildew thalline and the conidium killed in soil, eliminate major part
Pathogeny, the incidence of disease are low；It was found from the 3rd group contrasts with control group 2, each growth phase of planting process of the present invention uses plant-source antibacterial
The preventing and treating of how clever bacterium of the prevention effect of agent better than 50%, plant-source antibacterial agent of the invention can reduce the drug resistance of pathogenic bacteria,
Reduce recurrent number；It is purple common perilla seed in plant-source antibacterial agent of the present invention, diligent it was found from the 3rd group contrasts with control group 3
Right seed extract and fulvic acid Synergistic, strengthen drug effect, make inhibition more preferable.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (6)
- A kind of 1. biological control method of powdery mildew of strawberry, it is characterised in that：Comprise the following steps：(1) first by Soil tillage before strawberry cultivating, bedding a layer thickness is 2-4cm moistening straw on soil after turning over, and is spread After lid terminates, the complex microorganism matrix of 10-20kg/ mus is uniformly sprinkling upon on straw, and one layer is covered in antibacterial matrix Thickness is 1-3cm fine earth, watering, overlay film, in coating process every 6-8h to membrane gas treatment once, can be removed after 4-6 days Film plants strawberry；The complex microorganism matrix is mainly mixed by the raw material of following parts by weight：Edible fungi residues 20-23 Part, Pseudomonas aeruginosa 3-5 parts, bacillus subtilis 6-8 parts, streptomycete 1-3 parts, Paecilomyces lilacinus 2-4 parts and saccharomycete 9-11 Part；(2) strawberry of each growth phase is sprayed with plant-source antibacterial agent；Seedling Stage sprays once, and each amount of application is 20-50mL/ mus；Vigorous period was applied once every 5-7 days, and each amount of application is 90-100mL/ mus；Florescence is every 3-5 It is applied once, and each amount of application is 70-90mL/ mus；The Chinese olive phase applied once every 2-4 days, and each amount of application is 65- 75mL/ mus；The haw phase applied once every 2-3 days, and each amount of application is 50-65mL/ mus, you can effectively prevents and treats powdery mildew；The plant-source antibacterial agent is mainly mixed by the raw material of following parts by weight：Purple common perilla seed 10-15 parts, cumin Seed extract 8-11 parts, fulvic acid 4-6 parts and aqueous emulsion 6-9 parts；The preparation method of the purple common perilla seed is：Fresh purple perilla seed is dried to constant weight at 80-85 DEG C, clayed into power, After powder is mixed with the water of 2-3 times of weight, added into compound the powder weight 0.2-0.3% cellulase, 0.1-0.2% pectase and 0.15-0.25% protease mix, and adjustment pH is 4-5, and 2-3h, mistake are reacted at 45-55 DEG C Filter, filtrate is centrifuged, takes supernatant liquor, produces the purple common perilla seed；The preparation method of the cumin seed extract is：Fresh cumin seed is dried to constant weight by A at 70-75 DEG C, and is ground Into powder, after the powder and water mixed in equal amounts, the microwave treatment 30-60s under 800-1000W is standby；B is to microwave treatment The ethanol solution that 5-7 times of weight is added in compound afterwards mixes, and refluxing extraction 2-3 times at 90-100 DEG C, flow back 1- every time 2h, merge ethanol extract, produce the cumin seed extract.
- A kind of 2. biological control method of powdery mildew of strawberry according to claim 1, it is characterised in that：In step (1), institute The water content for stating moistening straw is 50-60%.
- A kind of 3. biological control method of powdery mildew of strawberry according to claim 1, it is characterised in that：In step (1), institute Complex microorganism matrix is stated mainly to be mixed by the raw material of following parts by weight：It is 22 parts of edible fungi residues, 4 parts of Pseudomonas aeruginosa, withered 10 parts of careless 7 parts of bacillus, 2 parts of streptomycete, 3 parts of Paecilomyces lilacinus and saccharomycete.
- A kind of 4. biological control method of powdery mildew of strawberry according to claim 1, it is characterised in that：In step (2), institute Plant-source antibacterial agent is stated mainly to be mixed by the raw material of following parts by weight：13 parts of purple common perilla seed, the extraction of cumin seed 7 parts of 10 parts of thing, 5 parts of fulvic acid and aqueous emulsion.
- A kind of 5. biological control method of powdery mildew of strawberry according to claim 1, it is characterised in that：In step (2), institute State and diluted before plant-source antibacterial agent is applied every time with 100-200 times of water.
- A kind of 6. biological control method of powdery mildew of strawberry according to claim 1, it is characterised in that：In step (2), institute It is 1000-1200r/min, centrifugation time 20-25min to state centrifugal rotational speed.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234172A (en) * | 2018-10-18 | 2019-01-18 | 天津大学 | A kind of complex micro organism fungicide for preventing and treating Powdery Mildew of Euonymus japonicus L |
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