CN107593431B - A kind of breeding method of type high oleic acid peanut varieties of crawling of continuously yielding positive results - Google Patents
A kind of breeding method of type high oleic acid peanut varieties of crawling of continuously yielding positive results Download PDFInfo
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- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 title claims abstract description 63
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 title claims abstract description 63
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 title claims abstract description 63
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 title claims abstract description 63
- 239000005642 Oleic acid Substances 0.000 title claims abstract description 63
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 title claims abstract description 63
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 title claims abstract description 63
- 230000009193 crawling Effects 0.000 title claims abstract description 54
- 235000017060 Arachis glabrata Nutrition 0.000 title claims abstract description 48
- 241001553178 Arachis glabrata Species 0.000 title claims abstract description 48
- 235000010777 Arachis hypogaea Nutrition 0.000 title claims abstract description 48
- 235000018262 Arachis monticola Nutrition 0.000 title claims abstract description 48
- 235000020232 peanut Nutrition 0.000 title claims abstract description 48
- 238000009395 breeding Methods 0.000 title claims abstract description 27
- 241000196324 Embryophyta Species 0.000 claims abstract description 81
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 16
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 14
- 101150076489 B gene Proteins 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims abstract description 8
- 101710088194 Dehydrogenase Proteins 0.000 claims description 19
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 17
- 229930195729 fatty acid Natural products 0.000 claims description 17
- 239000000194 fatty acid Substances 0.000 claims description 17
- 150000004665 fatty acids Chemical class 0.000 claims description 17
- 230000001488 breeding effect Effects 0.000 claims description 10
- 230000003321 amplification Effects 0.000 claims description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 4
- 238000013412 genome amplification Methods 0.000 claims description 2
- 238000003780 insertion Methods 0.000 abstract description 3
- 230000037431 insertion Effects 0.000 abstract description 3
- 101150058769 FAD2 gene Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 description 10
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 235000020778 linoleic acid Nutrition 0.000 description 4
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000011835 investigation Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 238000011160 research Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 101710161300 Protein peanut Proteins 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- VKOBVWXKNCXXDE-UHFFFAOYSA-N icosanoic acid Chemical compound CCCCCCCCCCCCCCCCCCCC(O)=O VKOBVWXKNCXXDE-UHFFFAOYSA-N 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- 241000894007 species Species 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
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Abstract
The present invention provides a kind of breeding method of type high oleic acid peanut varieties of crawling of continuously yielding positive results, and belongs to peanut varieties field.The breeding method will alternately yield positive results, creeping form parent and continuously yield positive results, oleic acid content 75% or more, upright type parent hybridizes or reciprocal cross；F1 generation is selfed to obtain F2 generation, selection continuously is bloomed, the type single plant of crawling expands the FAD2 gene of the single plant, is selected in A genome FAD2A gene at 448bp bases G to sport base A and 1 B gene the group single plant of base A insertion at 442bp and is high oleic acid, continuously blooms, type of crawling single plant；The single plant is selfed, selection is continuously bloomed, type of crawling, high oleic acid take into account that pod is full, the single plant more than single plant fruit；In F4~F7 generation, is selfed respectively, selection target character, and selection selfing obtains the single plant that selection yield in F8 generation increases production 10% or more than erect type parent.
Description
Technical field
The invention belongs to new peanut variety technical fields, and in particular to a kind of type high oleic acid peanut of crawling of continuously yielding positive results
The breeding method of kind.
Background technique
Peanut is the important oil crops and industrial crops in China, to the adjustment structure of rural undertaking, tool of increasing farmers' income
It plays an important role.Peanut varieties type in China's divides five major class at present, and the peanut type overwhelming majority used in main producing region is that plant type is straight
Vertical, perpetual intermediate form；Because of erect type kind, solid range is small, to obtain high yield, only increases planting density,
So sowing quantity is very big, seed costs is very high.
Oleic acid is a kind of monovalent unsaturated fatty acid, and linoleic acid is bivalent unsaturated fatty acid.Arachic acid and linoleic acid
The sum of account for 80% or so of Fatty Acid In Peanut total amount, oleic acid is more, and linoleic acid is just few.Research it has been shown that oleic acid content height not only
The oxidative rancidity speed of peanut and its product can be slowed down, and the low density cholesterol in blood to human health can be reduced
Content, while not influencing the content of the high density cholesterol beneficial to human health.In order to reduce seed costs, while quality
Good, beneficial to health of human body after eating, the peanut varieties that cultivation is continuously yielded positive results, yield is high, oil-producing acid is high are very necessary.
Currently, the parent that the common method of peanut varieties breeding is the required character of selection hybridizes, for example, application is public
The number of opening is that the patent of CN104813921 is disclosed using more grain shape parents and the low temperature resistant parents of high yield, and offspring is selfed
To the strain of yield increased volume increase 10%, high yield more new peanut varieties are obtained；Application publication number is CN102283098's
Patent is disclosed using high oleic acid and the hybridization of high protein peanut varieties, and offspring is selfed to obtain the peanut of high yield, high oleic acid high protein
Kind, wherein high oleic acid yield reaches 54.6%~58.2%.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of trainings of type high oleic acid peanut varieties of crawling of continuously yielding positive results
Method is educated, the peanut varieties that the cultural method is cultivated have the characteristics that peanut yield is high, oleic acid content is high.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of breeding methods of type high oleic acid peanut varieties, including following steps of crawling of continuously yielding positive results
It is rapid:
1) it alternately yields positive results, creeping form or from alternately yielding positive results, the parent of type of crawling offspring and continuously open
Flower result, oleic acid content hybridize or reciprocal cross in the parent of 75% or more, upright type, obtain F1 filial generation；
2) the F1 filial generation that the step 1) obtains is selfed to obtain F2 filial generation, selection continuously blooms, type of crawling single plant；
3) continuously blooming in the step 2), the 12 fatty acid dehydrogenase gene sequence of Δ of type of crawling single plant, choosing are expanded
It selects 12 fatty acid dehydrogenase gene bases G at 448bp of A genome Δ and sports base A, while 12 fatty acid of 1 B gene group Δ
The single plant that dehydrogenase gene has base A to be inserted at 442bp is high oleic acid, continuously blooms, type of crawling single plant；
4) bloom by high oleic acid obtained in the step 3), continuously, the type single plant of crawling is selfed, obtain F3 filial generation,
Selection is continuously bloomed, type of crawling, high oleic acid take into account that pod is full, the peanut single plant more than single plant fruit；
5) F4~F7 filial generation, selection target character are obtained after per generation selfing；The objective trait is continuously to bloom, is high oily
Acid, and single plant fruit is more, pod is full, character stable and consistent；
6) F7 filial generation is selfed to obtain F8 filial generation, selects yield to increase production 10% or more than erect type parent from F8 filial generation
Single plant, type high oleic acid peanut varieties of crawling of continuously being yielded positive results.
Preferably, the high oil product system in parent Hebei in the step 1), the high oil product system in Hebei from Guangdong oil 551 with
The filial generation of SPI210555, the SPI210555 be alternately yield positive results, the peanut wild species of creeping form.
Preferably, it continuously yields positive results in the step 1), the parent that oleic acid content is 76.8% is to open agriculture 17-6.
Preferably, the step 2), step 4), further include rejecting false hybrid and bad in step 5) and step 6) before selfing
Strain.
Preferably, in the step 3) 12 fatty acid dehydrogenase gene of A genome amplification Δ primer be FAD2-1A-F and
FAD2-R。
Preferably, the primer of 1 B gene group amplification 12 fatty acid dehydrogenase gene of Δ is FAD2-1B-F and FAD2-R.
Preferably, the quantity of fruit is 60~80 in every plant of peanut single plant in the step 4).
Preferably, it includes plant height, plant type and breeding time economical character that character, which is stablized, in the step 5).
The present invention provides a kind of breeding method of type high oleic acid peanut varieties of crawling of continuously yielding positive results, cultivation is spent
Health product kind have continuously yield positive results, type of crawling, the character of high oleic acid.Erect type plant range of results is small, to obtain high yield,
Density must be increased, causes seed dosage big, cost is just high；If being only the type of crawling, rather than continuously bloom, although can be with
Reach small density, reduce the purpose of seed costs, but due to being alternately to bloom, does not as a result concentrate, be easy to cause low output, and
Bad harvest.The present invention will continuously yield positive results, type of crawling, the character of high oleic acid concentrate breeding into new peanut variety, can
Guarantee the character for guaranteeing high oleic acid while high yield.
Meanwhile the new peanut variety that breeding method provided by the invention obtains, oleic acid yield is 81.3%, compared with the prior art
The oleic acid content of middle high oleic acid peanut varieties improves 48.9%.The new peanut variety flower that breeding method provided by the invention obtains
Educate 917 in the northern panel region test in the whole nation in 2016, performance continuously yields positive results, plant type of crawling, 378.6 public affairs of average yield per mu pod
Jin, than 336.5 kilograms of volume increase 12.5% that erect type control flower educates No. 33, tentative famous flower educates 9120.From same combination breeding
Flower educates 917 in the highly dense 120 mu of simple grains light seeding experiment and demonstration in Shandong in 2017, and per mu yield reaches 543.6 kilograms, than upright
Type high yield check variety flower educates 33 479.4 kilograms of volume increase 13.4%.
Detailed description of the invention
Fig. 1 is FAD2A ' gene base situation at 448bp in embodiment 1；
Fig. 2 is FAD2B ' gene catastrophe at 442bp in embodiment 1.
Specific embodiment
The present invention provides a kind of breeding methods of type high oleic acid peanut varieties, including following steps of crawling of continuously yielding positive results
It is rapid:
1) alternately yield positive results, the parent of creeping form and continuously yield positive results, oleic acid content is in 75% or more, upright class
The parent of type hybridizes or reciprocal cross, obtains F1 filial generation；
2) the F1 filial generation that the step 1) obtains is selfed to obtain F2 filial generation, selection continuously blooms, type of crawling single plant；
3) continuously blooming in the step 2), the 12 fatty acid dehydrogenase gene sequence of Δ of type of crawling single plant, choosing are expanded
It selects 12 fatty acid dehydrogenase gene of A genome Δ (FAD2A ' gene) bases G at 448bp and sports base A (such as Fig. 1), together
When 12 fatty acid dehydrogenase gene (FAD2B ' gene) of 1 B gene group Δ there is the single plant of base A insertion (such as Fig. 2) to be at 442bp
High oleic acid is continuously bloomed, type of crawling single plant；
4) bloom by high oleic acid obtained in the step 3), continuously, the type single plant of crawling is selfed, obtain F3 filial generation,
Selection is continuously bloomed, type of crawling, high oleic acid take into account that pod is full, the peanut single plant more than single plant fruit；
5) F4~F7 filial generation is selfed respectively, selection target character；The objective trait be continuously bloom, high oleic acid, and
Single plant fruit is more, pod is full, character stable and consistent；
6) F7 filial generation is selfed to obtain F8 filial generation, selects yield to increase production 10% or more than erect type parent from F8 filial generation
Single plant, type high oleic acid peanut varieties of crawling of continuously being yielded positive results.
The present invention will alternately yield positive results, the parent of creeping form and continuously yield positive results, oleic acid content 75% or more,
The parent of upright type hybridizes, and obtains F1 filial generation.
In the present invention, it is described continuously yield positive results, oleic acid content 75% or more, upright type parent preferably open
Agriculture 17-6.The agriculture 17-6 that opens is from opening the miscellaneous of agriculture 30 (continuously blooming, osculant) and choice-start 01-6 (alternately blooming, high oleic acid)
Hand over offspring.The present invention is not particularly limited the source for opening agriculture 30 and choice-start 01-6, opens agriculture using known in the art
The peanut strain of 30 and choice-start 01-6.In the present invention, the peanut strain for opening agriculture 30 and choice-start 01-6 is from Henan
Save Kaifeng Academy of Agricultural Sciences.
In the present invention, the alternating is yielded positive results, the parent for type of crawling is preferably the high oil product system in Hebei.The high oil in the Hebei
For Guangdong oil 551 (continuously blooming, Pearl Type) and SPI210555 (alternating bloom, type of crawling) filial generation.The present invention is to described
The source of Guangdong 551 and SPI210555 of oil is not particularly limited, using the flower of 551 and SPI210555 of Guangdong oil known in the art
Health product system.In the present invention, the peanut strain of 551 and SPI210555 of the Guangdong oil is made from Guangdong Academy of Agricultural Sciences
Object research institute.
In the present invention, the alternating yields positive results, the parent of creeping form and continuously yield positive results, oleic acid content is 75%
Above, the parent of upright type carries out orthogonal or reciprocal cross.
In the present invention, the alternating yields positive results, the parent of creeping form and continuously yield positive results, oleic acid content is 75%
Above, the seed number of the parent of upright type is preferred must not independently be less than 60 pods.
The present invention is not particularly limited the method for the hybridization, using crossing scheme well-known to those skilled in the art
?.
After obtaining F1 filial generation, the present invention is selfed the F1 filial generation to obtain F2 filial generation, selects in the F2 filial generation continuous
It blooms, type of crawling single plant.
In the present invention, before selfing, it is also preferable to include reject false hybrid and weak plant.
The single plant that will appear four kinds of characters in obtained F2 filial generation, specially continuously blooms, erect type, and alternating is bloomed, directly
Vertical type continuously blooms, type of crawling and alternately blooms, four seed type of type of crawling, and selection is continuously bloomed, the type single plant of crawling is conducive to
To the filial generation of high yield and concentration of yielding positive results.
Continuously bloomed, after type of crawling single plant, the present invention expand it is described continuously bloom, 12 fat of the Δ of type of crawling single plant
Dehydrogenase gene sequence sports base A in 12 fatty acid dehydrogenase gene bases G at 448bp of A gene group selection Δ,
It is simultaneously high oleic acid, continuously in the single plant that 12 fatty acid dehydrogenase gene of 1 B gene group selection Δ has base A to be inserted at 442bp
It blooms, type of crawling single plant.
In the present invention, the primer of amplification FAD2A gene is FAD2-1A-F and FAD2-R；Amplification FAD2B gene primer be
FAD2-1B-F and FAD2-R.FAD2-1A-F, FAD2-1B-F and FAD2-R primer and operating process bibliography: peanut
AhFAD2-1 gene and oleic acid/linoleic acid ratio relationship peanut journal, 2016,45 (4): 20-24.
It expands obtained PCR product to be sequenced, the raw work in sequencing commission Shanghai is sequenced.Sequencing is obtained
FAD2A ' gene and FAD2B ' gene are compared respectively, and FAD2A ' gene (A genome) bases G at 448bp is selected to be mutated
The single plant for thering is base A to be inserted at 442bp for base A, simultaneous selection FAD2B ' gene (1 B gene group).
In the present invention, 12 fatty acid dehydrogenase gene bases G at 448bp of A genome Δ sports base A, together
When 12 fatty acid dehydrogenase gene of the 1 B gene group Δ single plant that there is base A to be inserted at 442bp, 75% or more oleic acid content.
Obtained high oleic acid is continuously bloomed, after type of crawling single plant, and the present invention blooms by the high oleic acid, continuously, type of crawling
Single plant is selfed, and obtains F3 filial generation, and selection is continuously bloomed, type of crawling, high oleic acid take into account that pod is full, the peanut more than single plant fruit
Single plant.
In the present invention, before the selfing, it is also preferable to include reject false hybrid and weak plant.
In the present invention, the quantity of fruit is preferably 60~80 in every plant of peanut single plant.
Per generation selfing F4~F7 filial generation respectively, selection target character；The objective trait be continuously bloom, high oleic acid, and
And single plant fruit is more, pod is full, character stable and consistent.
In the present invention, before per generation selfing, it is also preferable to include reject false hybrid and weak plant.The weak plant be with it is most of
The economical character difference of plant is big, such as pod quantity is few, plant type is inconsistent, flowering habit is different.
In the present invention, it includes plant height, plant type and breeding time economical character that the character, which is stablized,.
After obtaining F7 filial generation, F7 filial generation is selfed to obtain F8 filial generation by the present invention, selects yield to compare erect type from F8 filial generation
Parent increases production 10% or more single plant, type high oleic acid peanut varieties of crawling of continuously being yielded positive results.Before F7 filial generation selfing preferably
It further include rejecting false hybrid and weak plant.The weak plant be it is big with the economical character difference of most of plant, as pod quantity is few, strain
Type is inconsistent, flowering habit is different etc..
Below with reference to embodiment to a kind of training of type high oleic acid peanut varieties of crawling of continuously yielding positive results provided by the invention
It educates method to be described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
2008 Nian Yikai agriculture 17-6 (continuously blooming, osculant) --- from opening agriculture 30 (continuously blooming, osculant) and choice-start
The filial generation of 01-6 (alternating is bloomed, high oleic acid) is female parent；Hebei is high oily (continuously blooming, osculant) --- from " Guangdong oil
551 (continuously blooming, Pearl Type) and SPI210555 (alternating bloom, type of crawling) " filial generation is male parent composite-crossing, warp
High yield and high quality made of systematic breeding continuously yields positive results, type of crawling high oleic acid new peanut variety.Its Breeding Process is as follows:
In generation spring F0 in 2008, opens the high oil in the Hebei agriculture 17-6X.Shandong Laixi
Winter F1 generation in 2008 rejects false hybrid and weak plant, and the Hainan Province Hun Shou Sanya adds the generation selection of spring F2 in 2009 generation continuously to open
Flower, type of crawling single plant, and 12 fatty acid dehydrogenase gene of Δ (FAD2) sequence alignment is carried out to it.It is all to exist simultaneously FAD2A ' base
Because 448bp at bases G sport A (Fig. 1) and FAD2B ' gene to have the single plant of base A insertion (Fig. 2) at 442bp be exactly height
Oleic acid peanut is selected as F3 for material.Shandong Laixi
The single plants such as generation winter F3 in 2009 continuously blooms to the F2 material selection for substituting into choosing, type of crawling, fruit is more, fruit is full.Shandong
Laixi
In F4 generation in 2010, repeats the selection technique in F3 generation.Shandong Laixi
In F5 generation in 2011, repeats the selection technique in F3 generation, rejects weak plant, carries out mixed receipts to the single plant of stable and consistent.Shandong Lay
West
F6 is for strain Yield comparison within 2012.Reject weak plant.Shandong Laixi
F7 is for strain Yield comparison within 2013.Main investigation cell production, economical character；Resistance.Shandong Laixi
F8 is for variety test within 2014.Main investigation cell production, economical character.Shandong Laixi
F9 is for variety test within 2015.Main investigation cell production；Measure oleic acid content.Qingdao, Laixi
The northern panel region test in the whole nation in 2016, performance continuously yields positive results, plant type of crawling, oleic acid content 81.3%；It is flat
Equal 378.6 kilograms of pod of per mu yield, than 336.5 kilograms of volume increase 12.5% that erect type control flower educates No. 33, the peanut that breeding obtains
New varieties fix tentatively entitled flower and educate 9120.
917 are educated in the highly dense 120 mu of simple grains light seeding experiment and demonstration in Shandong in 2017 from the flower of same combination breeding, average mu
Yield reaches 543.6 kilograms, than 479.4 kilograms of volume increase 13.4% that erect type high yield check variety flower educates 33.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of breeding method of type high oleic acid peanut varieties of crawling of continuously yielding positive results, comprising the following steps:
1) will alternately yield positive results, the parent for type of crawling or from alternately yield positive results, the parent of type of crawling offspring and continuous
Yield positive results, oleic acid content 75% or more, erect type parent carry out orthogonal or reciprocal cross, obtain F1 filial generation；
2) the F1 filial generation that the step 1) obtains is selfed to obtain F2 filial generation, selection continuously blooms, type of crawling single plant；
3) continuously blooming in the step 2), the 12 fatty acid dehydrogenase gene sequence of Δ of type of crawling single plant are expanded, A base is selected
Because 12 fatty acid dehydrogenase gene of group Δ bases G at 448bp sports base A, while 12 fatty acid dehydrogenase of 1 B gene group Δ
The single plant that gene has base A to be inserted at 442bp is high oleic acid peanut；
4) bloom by high oleic acid obtained in the step 3), continuously, the type single plant of crawling is selfed, selection continuously bloom, crawl
Crawl type, high oleic acid take into account that pod is full, the peanut single plant more than single plant fruit is as F3 filial generation；
5) F4~F7 filial generation obtained after per generation selfing, selection target character；The objective trait be continuously bloom, high oleic acid,
And single plant fruit is more, pod is full, character stable and consistent；
6) F7 filial generation is selfed to obtain F8 filial generation, yield is selected to increase production 10% or more list than erect type parent from F8 filial generation
Strain, type high oleic acid peanut varieties of crawling of continuously being yielded positive results.
2. breeding method according to claim 1, which is characterized in that the step 1) continuously yields positive results, oleic acid content
It is to open agriculture 17-6 in 75% or more, erect type parent.
3. breeding method according to claim 1, which is characterized in that the step 2), step 4), step 5) and step 6)
It further include rejecting false hybrid and weak plant before middle selfing.
4. breeding method according to claim 1, which is characterized in that 12 fat of A genome amplification Δ in the step 3)
The primer of dehydrogenase gene is FAD2-1A-F and FAD2-R.
5. breeding method according to claim 1, which is characterized in that 12 fat of 1 B gene group amplification Δ in the step 3)
The primer of dehydrogenase gene is FAD2-1B-F and FAD2-R.
6. breeding method according to claim 1, which is characterized in that the peanut single plant of every plant of F3 filial generation in the step 4)
The quantity of middle fruit is 60~80.
7. breeding method according to claim 1 or 6, which is characterized in that character includes plant height, plant type in the step 5)
With breeding time economical character.
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| CN101513170B (en) * | 2009-03-23 | 2011-10-26 | 山东省花生研究所 | Breeding method for high-oleic acid high-yield peanut |
| CN102283098B (en) * | 2011-07-05 | 2013-01-09 | 山东省花生研究所 | High-oleic-acid, high-protein and high-yield peanut breeding method |
| CN102283099B (en) * | 2011-07-06 | 2012-10-31 | 山东省花生研究所 | Method for breeding high-yield high-oleic-acid high-oil peanuts |
| CN104813921A (en) * | 2015-04-28 | 2015-08-05 | 吉林省农业科学院 | New variety breeding method for high-yield Valencia peanuts |
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| CN107593431A (en) | 2018-01-19 |
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