CN107582866A - Dendrobium candidum and Amlodipine are preparing the application process in treating hypertension drug - Google Patents

Abstract

The present invention relates to pharmaceutical formulation techniques, it is desirable to provide a kind of dendrobium candidum and Amlodipine are preparing the application process in treating hypertension drug.Including：2.5 parts of 8000 parts of dendrobium candidum and Amlodipine are taken in parts by weight；By dendrobium candidum be ground to it is powdered after, add Amlodipine powder, mix；Then auxiliary material is added according to pharmacy Conventional processing methods, tablet, particle or mix suspension grain, pill, powder or capsule is made, obtains the medicine for treating hypertension.The present invention has taken into full account the utilization of dendrobium candidum valuable medicinal, reduces the waste caused by extraction not exclusively, while also reduces destruction of the heating extraction process to fractions, to improve curative effect, saves valuable medicinal.The reduction of blood pressure in high-speed of 2 hours after can both being taken medicine, 24 hours antihypertensive effects exceed Western medicine depressor Amlodipine taken medicine again after, can also reduce the dosage of Western medicine Amlodipine.Greatly extend the application prospect of dendrobium candidum treatment hypertension.

Description

Application method of dendrobium officinale and amlodipine in preparation of medicine for treating hypertension

technical field

The invention relates to medicine preparation technology, in particular to the application of dendrobium officinale and amlodipine in the preparation of medicines for treating hypertension.

Background technique

Hypertension is a common high incidence clinically, which has serious harm to the heart, brain, kidney and other important organs. my country's 2013 "China Cardiovascular Disease Report" estimates that there are currently about 270 million hypertensive patients nationwide. The 2012 and 2013 "China Cardiovascular Disease Report" showed that the mortality rate of cardiovascular disease in my country was 44.1%, ranking first in the spectrum of death diseases. Hypertension is the leading cause of cardiovascular and cerebrovascular diseases. Epidemiological survey results show that the mortality rate of cardiovascular and cerebrovascular diseases in my country is increasing year by year due to high blood pressure. As far as stroke is concerned, there are more than 1.6 million new strokes in my country every year, and the cumulative number of survivors is about 6 million, of which more than 75% are lost. 45% of the labor force is severely disabled and cannot take care of themselves.

Western medicine treatment of hypertension still lacks the medicine for adjusting body constitution and can adjust blood pressure (depressor). The antihypertensive treatment of modern medicine is all symptomatic treatment. Currently commonly used antihypertensive drugs include calcium channel blockers (CCBs), angiotensin-converting enzyme inhibitors (ACEIs), angiotensin II receptor blockers (ARBs), diuretics, and β-blockers. , still can not completely prevent the progression of hypertension and its secondary lesions. Most hypertensive patients need two or more antihypertensive drugs to reach the target blood pressure, or it is difficult to reach the target blood pressure. Most patients need to take the medicine for life. These problems become more prominent with the prolongation of life expectancy. At present, there is still an urgent need for new and better therapeutic drugs in the treatment of hypertension.

The current traditional Chinese medicine conditioning methods have certain advantages for hypertension, but there are also serious shortcomings. Although traditional Chinese medicine regulates high blood pressure, the speed and magnitude of lowering blood pressure are not as fast as that of western medicine, but it has the advantages of more stable and long-lasting curative effect, less blood pressure recovery after drug withdrawal, and the advantages of controlling blood pressure and improving overall physical fitness. Or it can reduce blood pressure while regulating blood pressure, etc., which has the advantages of improving various pathological indicators after adjusting and improving physical fitness, and has certain application value. However, there are still some problems that the reduction of blood pressure is not obvious enough in the near future after taking the medicine, the number of medicines has too many flavors to restrain each other or offset the effect, and the medicine composition, extraction, preparation process, etc. have not been systematically optimized, so the curative effect cannot meet the requirements, and it is difficult to be practical in clinical practice. Value.

When those skilled in the art apply Dendrobium candidum to the treatment of hypertension, there is a common bias in technical understanding: they think that only by using a large amount of water for extraction can the absorption, utilization and curative effect of the active ingredients of the drug be improved. Therefore, the traditional Tiepi There is a method of "wide soup and long decoction" in the usage of Dendrobium to improve the curative effect. Similarly, there have been patents for the preparation of drugs for the prevention or treatment of high blood pressure and stroke by using the water extract of Dendrobium candidum, or for the preparation of drugs for the prevention or treatment of high blood pressure and stroke by using the water extract of Dendrobium candidum in combination with nimodipine. However, the present invention overcomes the traditional prejudice. In the treatment of hypertension, Dendrobium officinale adopts the powder application method, which can greatly improve the 24-hour long-acting antihypertensive effect than the water extraction method, and the 24-hour antihypertensive effect after taking the medicine It can exceed the western medicine antihypertensive drug amlodipine (adult 5mg/day), and has obtained unexpected curative effect; After the rapid blood pressure reduction in the last 2 hours, the antihypertensive effect in 24 hours after taking the medicine exceeds that of Western medicine antihypertensive drug amlodipine (5mg/day for adults), and the consumption of Western medicine amlodipine can also be reduced.

Contents of the invention

The technical problem to be solved by the present invention is to overcome the deficiencies and prejudices in the prior art, and provide an application method of dendrobium officinale and amlodipine in the preparation of drugs for treating hypertension.

For solving technical problem, solution of the present invention is:

Provide a kind of application method of Dendrobium officinale and amlodipine in the preparation of medicine for treating hypertension, it is characterized in that, comprising: taking 8000 parts of Dendrobium officinale and 2.5 parts of amlodipine in parts by weight, Dendrobium officinale complies with the 2015 edition of "Chinese Pharmacopoeia" "Requirements in "; After grinding Dendrobium officinale into powder, add amlodipine powder, mix; then add auxiliary materials according to pharmaceutical conventional processing methods, make tablets, granules or suspension granules, pills, powders or capsules, and obtain Medicines used to treat high blood pressure.

The present invention further provides a method for using the drug for treating hypertension prepared in the aforementioned method, which is to administer the prepared tablet, granule or suspension granule, pill, powder or capsule orally, and the daily dosage for an adult is equivalent to that of iron sheet The crude drug amount of Dendrobium is 4g to 8g, and contains 1.25mg to 2.5mg of amlodipine.

Realization principle of the present invention:

According to TCM theory, most of hypertension belongs to deficiency of yin and hyperactivity of yang, and nourishing yin and subduing yang is the main treatment direction.

According to the theory and practice of traditional Chinese medicine, referring to the 2015 edition of the Pharmacopoeia of the People's Republic of China: the properties of Dendrobium officinale are to nourish the stomach and promote body fluid, nourish yin and clear away heat. It can be used for febrile disease, fluid injury, dry mouth and polydipsia, insufficient stomach yin, lack of food and retching, persistent asthenic fever after illness, yin deficiency and hyperactivity of fire, bone steaming and labor heat, blurred vision, muscle flaccidity and weakness. It is also used in the treatment of hypertension in the prior art, but it has not been applied on a large scale because of its slow onset of action and unsatisfactory antihypertensive effect.

The usual method of using Dendrobium officinale is limited by the traditional "wide soup and long decoction". The method of "pounding" the herbal medicine of Dendrobium officinale when dispensing in a pharmacy is directly added to decocting and extracting for clinical application. For this usage, because the main components of the medicinal material are not easy to be decocted, the proportion of its active ingredients (such as: polysaccharide) is very low (about less than 10% of the weight of the medicinal material can be extracted to obtain polysaccharides), and it is used for the curative effect of lowering blood pressure. Not ideal either. Afterwards, the improved extraction method is to use a machine to "crush" the medicinal material of Dendrobium officinale to extract, and the proportion of active ingredients (such as: polysaccharide) has been increased to a certain extent (about 20% of the weight of the medicinal material can be extracted). The curative effect when used for reducing blood pressure is also improved to a certain extent, and this method is mostly used in industrialized production of Chinese patent medicines. (see Experiment 1).

In the research work done by the present invention, the above-mentioned extraction method has been greatly improved: adopt a machine to "grind powder" the medicinal material of Dendrobium candidum, then add water to extract, adopt solid-liquid separation technology, separate the extract, and its active ingredients (such as: Polysaccharides) Proposed ratio has been greatly improved (about 40% polysaccharides can be extracted to obtain the medicinal material weight. Once carried out the extraction experiments of the above Dendrobium officinale medicinal pieces "smashing flat", "crushing", "grinding powder", all using the same batch Medicinal materials are compared), and the curative effect when used for lowering blood pressure has been improved to a certain extent. This method is only initially adopted in the research on lowering blood pressure of Dendrobium officinale, and has not been practically applied. However, it was found in the research that although the antihypertensive effect of the water extraction method after "grinding powder" is better than that of the "crushing" and "smashing flat" methods, there is still a long way to go before reaching the antihypertensive effect of the western drug amlodipine. ; At the same time, it was found that the 24-hour antihypertensive effect and blood pressure stability of Dendrobium officinale "ground powder" were better.

The method of directly taking Dendrobium officinale after "grinding powder": the utilization of drug components is more complete than that of water extraction, and the drug will gradually play a role after digestion and absorption in the body, which is equivalent to the "sustained release" effect of Chinese medicinal materials in the process of lowering blood pressure in the body, reaching The 24-hour antihypertensive effect is more stable. (see experiment 1, experiment 2).

The present invention overcomes the traditional prejudice: the application method of grinding powder can greatly improve the 24-hour long-acting antihypertensive effect compared with the water extraction method, and the effect in this aspect can be similar to that of the western medicine antihypertensive drug amlodipine (5 mg/day for adults). , its antihypertensive effect 24 hours after taking the medicine can surpass amlodipine, and obtain unexpected curative effect; The 24-hour antihypertensive effect after taking the medicine exceeds that of western medicine antihypertensive drug amlodipine (adult 5mg/day), and can also reduce the consumption of western medicine amlodipine.

Compared with prior art, the beneficial effect of the present invention is:

1, the present invention adopts Chinese medicine Dendrobium officinale powder to make tablet, granule, pill, powder or capsule and is conducive to exerting lasting and stable drug effect: (1) hypertension treatment needs medicine to bring into play persistent and stable drug effect, and western medicine reduces Pressed medicine adopts slow-release dosage form, and the present invention adopts medicinal material fine powder to make tablet, granule (suspension granule), pill, powder, or capsule to play the "ball person's slowness" that traditional Chinese medicine says, so that the drug is gradually stripped out and brought into play. (2) Dendrobium officinale powder raw (different from the use of "Jiujian"): it also has a good effect of "clearing heat" and "removing fire", which is beneficial to blood pressure (3) Dendrobium officinale is a precious medicinal material that needs to be fully utilized: the technical scheme of the present invention fully considers the utilization of the precious medicinal material of Dendrobium candidum, reduces the waste caused by incomplete extraction, and also reduces the damage to some components during the heating extraction process, To improve the curative effect and save precious medicinal materials.

2, Dendrobium officinale powder of the present invention is used in combination with Western medicine antihypertensive drug amlodipine, can not only obtain rapid blood pressure reduction in 2 hours after taking the medicine, but also obtain the antihypertensive effect in 24 hours after taking the medicine than Western medicine antihypertensive drug Amlodipine (5 mg for adults). /day), can also reduce the dosage of western medicine amlodipine.

3. Dendrobium candidum regulates Yin-deficiency and Yang-excessive physique and treats high blood pressure as a "basic cure" method: it can not only achieve the curative effect of lowering blood pressure quickly, but also obtain better medium and long-term stable blood pressure, and adjust and restore the balance of Yin and Yang. . The traditional Chinese medicine can obtain the curative effect similar to that of western antihypertensive medicine, which greatly expands the application prospect of Dendrobium officinale in treating hypertension.

detailed description

Example 1

Get Dendrobium candidum 800g by the requirement in 2015 edition " Chinese Pharmacopoeia ", get amlodipine besylate 250mg. The Dendrobium candidum medicinal material is ground into fine powder, amlodipine besylate powder is added, after mixing, appropriate excipients are added according to pharmaceutical routines to make granules (suspension granules), and dried. For Example 1 sample. Oral administration, the daily dosage for adults is equivalent to 4g of crude drug of Dendrobium candidum (containing 1.25mg of amlodipine) to 8g of crude drug of Dendrobium candidum (containing of 2.5mg of amlodipine).

Example 2

Get Dendrobium candidum 800g by the requirement in 2015 edition " Chinese Pharmacopoeia ", get amlodipine besylate 250mg. The Dendrobium candidum medicinal material is ground into fine powder, amlodipine besylate powder is added, after mixing, appropriate excipients are added according to pharmaceutical routines, and tablets are made. Be embodiment 2 samples. Oral administration, the daily dosage for adults is equivalent to 4g of crude drug of Dendrobium candidum (containing 1.25mg of amlodipine) to 8g of crude drug of Dendrobium candidum (containing of 2.5mg of amlodipine).

Experiment 1

Experimental study on the antihypertensive effect of Dendrobium officinale extraction method on hereditary hypertensive rats (SHR)

【Experimental Materials】

Rats: hereditary hypertensive rats (SHR), and Wistar rats as normal controls.

Experimental Chinese medicine: The following three extraction methods of Dendrobium candidum used the same batch of medicinal materials.

Extraction of Dendrobium officinale medicinal material decoction pieces after "pounding": take 500g of Dendrobium officinale in accordance with the 2015 edition of "Chinese Pharmacopoeia". Use a common medicine mallet to "pound" and extract with water. The first water extraction: add water 30 times the amount of crude drugs, extract at 100 ° C for 2 hours; the second water extraction: add water 20 times the amount of crude drugs, Extract at 100°C for 2 hours. The water extracts are combined and concentrated, and refrigerated for later use. Compared with the usual traditional Chinese medicine decoction, this extraction method greatly increases the amount of extracted water. After the determination of the polysaccharide content by the sulfuric acid phenol method stipulated in the Pharmacopoeia, the polysaccharide content of the obtained sample is 9.8% of the crude drug content of the medicinal material Dendrobium candidum;

Dendrobium candidum herbal pieces are "crushed" and extracted with water: take 500g of Dendrobium candidum that complies with the 2015 edition of "Chinese Pharmacopoeia". Water extraction after "crushing" by machine, the first water extraction: adding water 30 times the amount of crude drugs, extracting at 100°C for 2 hours; the second water extraction: adding water 20 times the amount of crude drugs, 100°C Extract for 2 hours. The water extracts are combined and concentrated, and refrigerated for later use. Compared with the usual traditional Chinese medicine decoction, this extraction method greatly increases the amount of extraction water, and there is an additional "crushing" process before extraction. After the determination of the polysaccharide content by the sulfuric acid phenol method stipulated in the Pharmacopoeia, the polysaccharide content of the obtained sample is 20.3% of the crude drug amount of the medicinal material Dendrobium officinale;

Extraction of Dendrobium officinale medicinal herbal pieces "ground into powder" and water: take 500g of Dendrobium officinale in accordance with the 2015 edition of "Chinese Pharmacopoeia". Water extraction after "grinding powder" by machine, the first water extraction: add water 30 times the total amount of crude drug, extract at 100°C for 2 hours; second water extraction: add water 20 times the amount of crude drug, 100°C Extract for 2 hours. The water extracts are combined and concentrated, and refrigerated for later use. Compared with the usual traditional Chinese medicine decoction, this extraction method greatly increases the amount of extraction water, and there is an additional process of "grinding powder" before extraction. After the determination of the polysaccharide content by the sulfuric acid phenol method stipulated in the Pharmacopoeia, the polysaccharide content of the obtained sample is 40.5% of the crude drug content of Dendrobium officinale medicinal material;

Western antihypertensive control drug: Amlodipine Besylate Tablets (Norvasc), 5mg/tablet, batch number: R78401, produced by Pfizer.

Rat blood pressure measurement: BP-98A non-invasive tail artery blood pressure measuring instrument, Japan Soft Rong Co., Ltd.

【experimental method】

Grouping treatment: All rats were fed adaptively for one week and then measured blood pressure and body weight, and then grouped. Divide the SHR rats into the group of Dendrobium officinale herbal pieces "crushed" and water extraction group with crude drug amount 0.8g/Kg body weight, the group of Dendrobium officinale medicinal herb pieces after "crushing" and water extraction group with crude drug amount of 0.8g/Kg body weight, After "grinding powder", the amount of crude drug in the water extraction group was 0.8g/Kg body weight, and the amlodipine besylate tablet in the amlodipine group was 0.5 mg/Kg body weight, both were given by intragastric administration, and the hypertension model control group was intragastrically administered with the same amount of normal saline , the normal control group was given the same amount of normal saline. 8 rats in each group, treated for 2 weeks.

Blood pressure measurement: Preheat the rats at 38°C for 5-10 minutes, so as to prevent blood pressure from being measured due to low blood flow in the tail of the rats. The blood pressure of the rat was measured continuously for 3 times in an awake and quiet state, and the average value of the 3 times was taken as the blood pressure value of the rat. Blood pressure was measured once a week, and once 2 hours after administration.

【Experimental Results】

blood pressure data See Table 1 of Experiment 1 and Table 2 of Experiment 1.

After 2 weeks of treatment, the blood pressure at 2 hours after administration, the blood pressure of the ground powder water extraction group was 188.6±5.68/148.0±4.22mmHg, which was significantly lower than that of the model group 209.3±6.89/160.3±5.67mmHg (P<0.01/P<0.01), but Higher than the western medicine amlodipine group 175.5±4.48/143.7±4.28mmHg, the difference in systolic blood pressure was significant (P<0.05). The reduction in blood pressure in the pounded flat water extraction group and the crushed water extraction group was smaller than that in the ground powder water extraction group. The antihypertensive effect of Dendrobium candidum powder and water extraction sample is relatively good, but the antihypertensive effect is lower than that of the western medicine amlodipine.

The rat doses used in this experiment correspond to equivalent adult doses:

The corresponding adult dose of Dendrobium candidum decoction pieces "pounded" is about 8g/day for the water extraction group;

The corresponding adult dose of Dendrobium candidum decoction pieces "crushed" and water extraction group is about 8g/day of crude drug of Dendrobium candidum;

The corresponding adult dose of Dendrobium candidum decoction pieces "ground into powder" is about 8g/day for adults;

The corresponding adult dose of the amlodipine group is about 5 mg/day of amlodipine besylate tablet.

【in conclusion】

The antihypertensive effect of the water extraction group after "grinding powder" of Dendrobium decoction pieces is greater than that of the water extraction group after "crushing" of Dendrobium decoction pieces, and also greater than that of the water extraction group after "pounding" of Dendrobium decoction pieces, but its antihypertensive effect is lower than that of the western medicine ammonia Clodipine.

Experiment 1: Table 1. The influence of different extraction methods of Dendrobium candidum on the systolic blood pressure of SHR rats administered for 2 hours ( Unit: mmHg; n=8)

Note: Compared with the model group, △P<0.05▲P<0.01; compared with the other groups, ○P<0.05·P<0.01

Experiment 1: Table 2. The influence of different extraction methods of Dendrobium candidum on the diastolic blood pressure of SHR rats administered for 2 hours ( Unit: mmHg; n=8)

Note: Compared with the model group, △P<0.05▲P<0.01; compared with the other groups, ○P<0.05·P<0.01

Experiment 2

Experimental study on antihypertensive effect of Dendrobium officinale powder and two extracts of Dendrobium officinale in alcohol and water on hereditary hypertensive rats (SHR)

【Experimental Materials】

Rats: 10-week-old hereditary hypertensive rats (SHR), and Wistar rats as normal controls.

Experimental Chinese medicine:

Dendrobium officinale powder granule sample: Take 500g of Dendrobium officinale in accordance with the 2015 edition of "Chinese Pharmacopoeia". The Dendrobium candidum medicinal material is ground into fine powder, and suitable auxiliary materials are added according to pharmaceutical routines to make traditional Chinese medicine granules (suspension granules). drying. For Example 1 sample. Dissolve in hot water before use.

Dendrobium officinale extract granules with alcohol and water: Take 500g of Dendrobium officinale in accordance with the 2015 edition of "Chinese Pharmacopoeia", grind it into powder, add 75% ethanol 5 times the total crude drug amount, and extract at 60°C for 24 hours, extract twice, and the residue after alcohol extraction Then extract with water. Two gradient heating extraction methods were used for water extraction: the first water extraction: add water 30 times the total amount of crude drug, extract at 100 ° C for 2 hours; the second water extraction: add water 20 times the total amount of crude drug, 121 °C extraction for 2 hours. The aqueous extracts were combined and concentrated. After alcohol extraction and water extraction are respectively concentrated, the extracts are combined, and appropriate excipients are added according to pharmaceutical routines to make traditional Chinese medicine granules. drying. Be embodiment 2 samples. Dissolve in hot water before use.

Alcohol extraction sample: the same as the alcohol extraction part of Dendrobium officinale granule sample: Take 500g of Dendrobium officinale that conforms to the 2015 edition of "Chinese Pharmacopoeia", grind it into powder, add 75% ethanol that is 5 times the total crude drug amount, and extract at 60°C for 24 hours , extract twice, combine the extracts, concentrate, and set aside.

Water extraction sample: Take 500g of Dendrobium officinale that conforms to the 2015 edition of "Chinese Pharmacopoeia", grind it into powder, the first water extraction: add water 30 times the amount of crude drug, extract at 100°C for 2 hours; the second water extraction: add up 20 times the amount of crude drug in water, extracted at 100°C for 2 hours. The water extracts were combined, concentrated, and set aside.

Western antihypertensive control drug: Amlodipine Besylate Tablets (Norvasc), 5mg/tablet, batch number: R78401, produced by Pfizer.

Rat blood pressure measurement: BP-98A non-invasive tail artery blood pressure measuring instrument, Japan Soft Rong Co., Ltd.

【experimental method】

Grouping treatment: All rats were fed adaptively for one week and then measured blood pressure and body weight, and then grouped. SHR rats are divided into Dendrobium officinale alcohol extraction sample group crude drug amount 0.8g/Kg body weight, Dendrobium officinale water extraction sample group crude drug amount 0.8g/Kg body weight, Dendrobium officinale powder sample group crude drug amount 0.8g/Kg body weight, Dendrobium officinale The amount of crude drug in the alcohol and water extraction sample group was 0.8g/Kg body weight, and the amlodipine besylate tablet was 0.5mg/Kg body weight in the amlodipine group. The control group was intragastrically administered the same amount of normal saline. Eight rats in each group were treated for 8 weeks. Blood pressure was observed for 1 week after drug withdrawal.

Blood pressure measurement: Preheat the rats at 38°C for 5-10 minutes, so as to prevent blood pressure from being measured due to low blood flow in the tail of the rats. The blood pressure of the rat was measured continuously for 3 times in an awake and quiet state, and the average value of the 3 times was taken as the blood pressure value of the rat. Measure blood pressure twice a week, once 2 hours after administration, and once 24 hours after administration, to observe the immediate antihypertensive effect and 24-hour antihypertensive effect (as well as the peak-to-peak ratio of antihypertensive) after administration .

Calculation of valley-to-peak ratio of blood pressure reduction: the difference between blood pressure before treatment - blood pressure 24 hours after administration is the valley value of blood pressure reduction, that is, the lowest point of blood pressure reduction before the next administration; blood pressure before treatment - after administration The difference in blood pressure at 2 hours is the peak value of the blood pressure reduction range, that is, the point at which the blood pressure reduction range is maximum after administration; the valley value of the blood pressure reduction range/the peak value of the blood pressure reduction range = the ratio of the valley-peak reduction range of the blood pressure reduction range. The closer the peak-to-valley ratio of the antihypertensive amplitude is to 1, the more stable the antihypertensive effect is, and the more it meets the requirements of long-acting antihypertensive drugs; the closer the peak-to-valley ratio of the antihypertensive amplitude is to 0, the shorter the antihypertensive effect.

【Experimental Results】

blood pressure data See Table 1 of Experiment 2, Table 2 of Experiment 2, Table 3 of Experiment 2, Table 4 of Experiment 2, and Table 5 of Experiment 2.

1. Blood pressure at 1 week of treatment:

(1) Blood pressure 2 hours after administration:

Dendrobium officinale alcohol water extraction group (alcohol water extraction group) 172.9±9.38/141.1±10.53mmHg, very significantly lower than the model group 207.0±7.45/157.0±7.03mmHg (P<0.01/P<0.01), and lower than Alcohol extraction group 181.4±5.22/146.8±9.60mmHg (P<0.01/P>0.05), lower than water extraction group 180.0±7.26/143.7±7.56mmHg (P<0.01/P>0.05). It was also slightly lower than the 175.1±4.44/143.2±6.41mmHg of the western medicine antihypertensive drug amlodipine group, but there was no significant difference (P>0.05/P>0.05). The 2-hour antihypertensive effect of Dendrobium officinale alcohol and water extraction group (alcohol and water extraction group) has surpassed the water extraction group and alcohol extraction group in 1 week of medication, and has reached the lowering effect of the western medicine antihypertensive drug amlodipine group. pressure range.

Dendrobium candidum powder group (research powder group) 181.2±6.71/148.1±10.55mmHg, significantly lower than the model group (P<0.01/P<0.05), but higher than the Dendrobium officinale alcohol-water extraction group (alcohol-water extraction group) The step-down range is small.

(2) Blood pressure 24 hours after administration:

Dendrobium candidum powder group (research powder group) 189.7±10.26/149.3±9.05mmHg, significantly lower than the model group 208.1±9.31/163.8±5.83mmHg (P<0.05/P<0.01), also compared with Dendrobium officinale Group (alcohol and water extraction group), water extraction group, alcohol extraction group, western medicine antihypertensive drug amlodipine group have a large range of blood pressure reduction.

Dendrobium officinale alcohol water extraction group (alcohol water extraction group) 203.2±12.69/161.5±13.54mmHg, lower than the model group 208.1±9.31/163.8±5.83mmHg, but no significant difference (P>0.05/P>0.05).

(3) Blood pressure reduction range for 24 hours after administration / blood pressure reduction range for 2 hours after administration = valley-peak ratio of blood pressure reduction range:

Dendrobium candidum powder group (powder research group) administration 24 hours blood pressure reduction range 20.8mmHg/administration 2 hours blood pressure reduction range 29.3mmHg = blood pressure reduction range valley-peak ratio 0.71;

In the water extraction group, the 24-hour blood pressure reduction range was 6.9 mmHg/the 2-hour blood pressure reduction range was 27.4 mmHg=the blood pressure reduction range valley-peak ratio was 0.25;

In the alcohol extraction group, the range of blood pressure reduction in 24 hours was 10.1 mmHg/the range of blood pressure reduction in 2 hours was 26.0 mmHg=the valley-peak ratio of blood pressure reduction was 0.39;

In the Western medicine amlodipine group, the 24-hour blood pressure reduction range was 9.1 mmHg/the 2-hour blood pressure reduction range was 34.7 mmHg=the blood pressure reduction range valley-peak ratio was 0.26;

Dendrobium officinale alcohol and water two extraction group (alcohol and water two extraction group) 24 hours after administration, the blood pressure reduction range is 3.8mmHg/administration 2 hours, the blood pressure reduction range is 34.1mmHg=the blood pressure reduction range valley-peak ratio is 0.11;

During 1 week of treatment, the 24-hour blood pressure reduction range of the Dendrobium candidum powder group (research powder group) was 20.8mmHg compared with 6.9mmHg in the water extraction group, 10.1mmHg in the alcohol extraction group, 3.8mmHg in the alcohol and water extraction group, and 3.8mmHg in the western medicine amlodipine. Dendrobium candidum powder group (research powder group) has a lower blood pressure range than 0.71 (71%) compared with 0.25 (25%) in the water extraction group, 0.39 (39%) in the alcohol extraction group, and 0.39 (39%) in the alcohol and water extraction group. 0.11 (11%) in the Western medicine amlodipine group and 0.26 (26%) in the western medicine amlodipine group, the peak-to-valley ratio of the antihypertensive amplitude was large, and the valley value of the antihypertensive amplitude reached more than 70% of the peak value of the antihypertensive amplitude, exceeding the Western medicine antihypertensive drug amlodipine. The Diping group (the valley-to-peak ratio of blood pressure reduction range is 26%) also exceeds the water extraction group of Dendrobium officinale (the valley-to-peak ratio of blood pressure reduction range is 25%). Dendrobium officinale research powder group (research powder group) was significantly better than the water extraction group and the Western medicine amlodipine group in the 24-hour blood pressure reduction range stability. Dendrobium officinale alcohol and water extraction group (alcohol and water two extraction group) has a 2-hour blood pressure lowering range of 34.1mmHg, which is significantly better than that of the water extraction group (27.4mmHg), reaching the range of 34.7mmHg of the Western medicine amlodipine group, and has a short-acting antihypertensive effect. superior to other groups.

2. Blood pressure at 8 weeks of treatment:

(1) Blood pressure 2 hours after administration:

Dendrobium officinale alcohol water two extraction group (alcohol water two extraction group) 172.2±2.22/134.7±3.41mmHg, very significantly lower than the model group 212.3±5.37/159.7±5.48mmHg (P<0.01/P<0.01), and lower than Alcohol extraction group 178.0±4.14/141.7±1.92mmHg (P<0.01/P<0.01), lower than water extraction group 178.2±2.90/142.3±3.28mmHg (P<0.01/P<0.01). It was also significantly lower than that of the western medicine antihypertensive drug amlodipine group (178.1±2.67/144.7±3.43mmHg, (P<0.01/P<0.01). The 2-hour antihypertensive effect of Dendrobium officinale alcohol and water extraction group (alcohol and water extraction group) was significantly higher than that of the water extraction group and alcohol extraction group during 8 weeks of medication, and exceeded the antihypertensive effect of the Western medicine antihypertensive drug amlodipine group The range of action, short-acting antihypertensive effect is better than other groups.

Dendrobium candidum powder group (research powder group) 180.6±2.91/145.0±4.06mmHg, significantly lower than the model group (P<0.01/P<0.01), but higher than the Dendrobium officinale alcohol-water extraction group (alcohol-water extraction group) The step-down range is small.

(2) Blood pressure 24 hours after administration:

Dendrobium candidum powder group (research powder group) 192.9±6.81/155.3±5.18mmHg, significantly lower than the model group 217.0±6.39/165.4±4.16mmHg (P<0.05/P<0.01), also significantly higher than Dendrobium candidum Extraction group (alcohol and water extraction group) 201.3±3.01/156.5±4.21mmHg, water extraction group 203.4±2.76/160.2±6.14mmHg, alcohol extraction group 202.0±4.39/156.0±8.39mmHg, Western medicine antihypertensive drug amlodipine group 202.0±5.88/154.3±5.50mmHg lowered the blood pressure significantly (P<0.01/P>0.05).

The Dendrobium candidum extract group (alcohol water extract group) was significantly lower than the model group (P<0.01/P<0.01), but the systolic blood pressure reduction range was smaller than that of the Dendrobium candidum powder group (research powder group).

(3) During 8 weeks of treatment, the 24-hour blood pressure reduction range/2-hour blood pressure reduction range = the blood pressure reduction range valley-peak ratio:

Dendrobium candidum powder group (research powder group) 24-hour blood pressure reduction range 17.6mmHg/administration 2-hour blood pressure reduction range 29.9mmHg = blood pressure reduction range valley-peak ratio 0.59;

In the water extraction group, the range of blood pressure reduction for 24 hours was 4.0 mmHg/the range of blood pressure reduction for 2 hours was 29.2 mmHg=the valley-peak ratio of blood pressure reduction range was 0.14;

In the alcohol extraction group, the range of blood pressure reduction in 24 hours was 5.4mmHg/the range of blood pressure reduction in 2 hours was 29.4mmHg=the valley-peak ratio of blood pressure reduction was 0.18;

In the western medicine amlodipine group, the range of blood pressure reduction in 24 hours was 7.8 mmHg/the range of blood pressure reduction in 2 hours was 31.7 mmHg=the valley-peak ratio of blood pressure reduction range was 0.25;

Dendrobium officinale alcohol and water two extraction group (alcohol and water two extraction group) 24 hours of administration, blood pressure reduction range 5.7mmHg/administration 2 hours blood pressure reduction range 34.8mmHg = blood pressure reduction range valley-peak ratio 0.16;

During 8 weeks of treatment, the 24-hour blood pressure reduction range of Dendrobium candidum powder group (research powder group) was 17.6mmHg compared with water extraction group 4.0mmHg, alcohol extraction group 5.4mmHg, alcohol and water extraction group 5.7mmHg, Western medicine amlodipine The lowering range of 7.8mmHg of the group is large, and the lowering range of the Dendrobium candidum powder group (ground powder group) has a valley-peak ratio of 0.59 (59%) compared with 0.14 (14%) of the water extraction group, 0.18 (18%) of the alcohol extraction group, and 0.18 (18%) of the alcohol extraction group. 0.16 (16%) in the alcohol and water extraction group, and 0.25 (25%) in the western medicine amlodipine group have a large valley-to-peak ratio of blood pressure reduction, and the valley value of the blood pressure reduction range reaches more than 50% of the peak value of the blood pressure reduction range, exceeding the Western medicine antihypertensive drug. The control drug amlodipine group (the peak-to-valley ratio of blood pressure reduction range was 25%) also surpassed the Dendrobium officinale water extraction group (the peak-to-peak ratio of blood pressure reduction range was 14%). Dendrobium officinale research powder group (research powder group) was significantly better than the water extraction group and the Western medicine amlodipine group in the 24-hour blood pressure reduction range stability. Dendrobium officinale alcohol and water extraction group (alcohol and water two extraction group) has a blood pressure reduction range of 34.8mmHg within 2 hours, which is significantly better than that of the water extraction group (29.2mmHg), exceeding the range of action of the Western medicine amlodipine group (31.7mmHg), and the short-acting antihypertensive effect better.

3. Blood pressure 1 week after drug withdrawal:

(1) Blood pressure 3 days after drug withdrawal:

Dendrobium officinale alcohol water extraction group (alcohol water extraction group) 198.8±3.12/153.4±5.01mmHg, very significantly lower than the model group 214.2±3.57/165.9±5.50mmHg (P<0.01/P<0.01), and significantly lower In the water extraction group 205.2±2.83/156.1±2.71mmHg (P<0.05/P<0.05), it was also significantly lower than the Western medicine antihypertensive drug amlodipine group 205.4±1.85/158.7±2.79mmHg, (P<0.05/P> 0.05). The antihypertensive effect of Dendrobium officinale two-extraction group (alcohol-water two-extraction group) 3 days after drug withdrawal was significantly higher than that of the water-extraction group and the Western medicine antihypertensive drug amlodipine group.

Dendrobium candidum powder group (research powder group) 199.6±3.24/152.1±5.77mmHg, significantly lower than the model group 214.2±3.57/165.9±5.50mmHg (P<0.01/P<0.01), compared with the Dendrobium candidum alcohol-water extraction group (Alcohol and water extraction group) the blood pressure reduction range is similar.

(2) Blood pressure 7 days after drug withdrawal:

The blood pressure in each group rose significantly compared with the treatment, and the diastolic blood pressure rose slowly compared with the systolic blood pressure.

The diastolic blood pressure of Dendrobium candidum extract group (alcohol and water extraction group) was 153.0±3.24mmHg, significantly lower than the water extraction group 165.5±3.13mmHg (P<0.01) and the alcohol extraction group 161.6±5.53mmHg (P<0.05).

The diastolic blood pressure of Dendrobium candidum powder group (powder research group) was 155.5±3.00mmHg, which was significantly lower than that of the water extraction group (P<0.05).

The western medicine antihypertensive drug amlodipine group lowered blood pressure quickly, but the blood pressure also rose quickly after stopping the drug. From the perspective of blood pressure 3 days after drug withdrawal, the blood pressure of the Dendrobium officinale powder group (research powder group) and the Dendrobium officinale alcohol-water extraction group (alcohol-water extraction group) rose slowly after drug withdrawal.

The rat doses used in this experiment correspond to equivalent adult doses:

Dendrobium candidum alcohol extraction sample group corresponds to a corresponding adult dose of about 8g/day of the crude drug dose of Dendrobium candidum;

Dendrobium officinale water extraction sample group corresponds to an equivalent adult dose of about 8g/day of the crude drug dose of Dendrobium officinale;

Dendrobium candidum powder group (Dendrobium candidum powder group) corresponds to a corresponding adult dose of about 8g/day of crude drug dose of Dendrobium candidum;

Dendrobium candidum alcohol water two extraction group (Dendrobium candidum alcohol water two extraction group) corresponds to an equivalent adult dose of about 8g/day of the crude drug dose of Dendrobium candidum;

The corresponding adult dose of the amlodipine group is about 5 mg/day of amlodipine besylate tablet.

【in conclusion】

Two hours of antihypertensive efficacy of Dendrobium officinale extract sample (alcohol water two extraction group) after administration, significantly better than Dendrobium officinale water extraction group, Dendrobium officinale alcohol extraction group, Dendrobium officinale powder group (research powder group), also It is better than the western medicine antihypertensive drug amlodipine group; the quick-acting antihypertensive drug reaches or surpasses the western medicine antihypertensive drug amlodipine; the blood pressure rises 3 days after stopping the drug is slower than the western medicine antihypertensive drug amlodipine; The sample is more suitable as a short-acting antihypertensive drug.

The antihypertensive curative effect of the Dendrobium candidum powder sample (research powder group) was significantly better than that of the Dendrobium candidum water extraction group, the Dendrobium candidum alcohol extraction group, and the Dendrobium candidum alcohol and water extraction group (alcohol and water extraction group). It is better than the western drug antihypertensive drug amlodipine group; the antihypertensive trough-peak ratio is large, and the antihypertensive stability reaches and exceeds that of the western drug antihypertensive drug amlodipine; Slow; Dendrobium candidum powder sample is more suitable as a long-acting antihypertensive drug.

Experiment 2: Table 1. Effects of Dendrobium officinale powder sample and Dendrobium officinale alcohol water extraction sample on the systolic blood pressure of SHR rats administered for 2 hours ( Unit: mmHg; n=8)

Note: △P<0.05▲P<0.01 compared with the model group; ○P<0.05 P<0.01 compared with the Dendrobium candidum powder group (ground powder) and each group; Compared with each group☆P＜0.05★P＜0.01

Experiment 2: Table 2. Effects of Dendrobium officinale powder sample and Dendrobium officinale alcohol water extraction sample on 24-hour systolic blood pressure of SHR rats ( Unit: mmHg; n=8)

Note: △P<0.05▲P<0.01 compared with the model group; ○P<0.05 P<0.01 compared with the Dendrobium candidum powder group (ground powder) and each group; Compared with each group☆P＜0.05★P＜0.01

Experiment 2: Table 3. Effects of Dendrobium officinale powder sample and Dendrobium officinale alcohol water extraction sample on diastolic blood pressure of SHR rats administered for 2 hours ( Unit: mmHg; n=8)

Note: △P<0.05▲P<0.01 compared with the model group; ○P<0.05 P<0.01 compared with the Dendrobium candidum powder group (ground powder) and each group; Compared with each group☆P＜0.05★P＜0.01

Experiment 2: Table 4. Effects of Dendrobium officinale powder sample and Dendrobium officinale alcohol water extraction sample on 24-hour diastolic blood pressure in SHR rats ( Unit: mmHg; n=8)

Note: △P<0.05▲P<0.01 compared with the model group; ○P<0.05 P<0.01 compared with the Dendrobium candidum powder group (ground powder) and each group; Compared with each group☆P＜0.05★P＜0.01

Experiment 2: Table 5. The influence of the Dendrobium officinale powder sample and the Dendrobium officinale alcohol-water extraction sample on the peak-to-peak ratio of the systolic blood pressure reduction range 24 hours after administration/2 hours after administration in SHR rats (before treatment-administration The valley value of the blood pressure drop 24 hours after the drug/before treatment-the peak value of the blood pressure drop 2 hours after the administration = the peak-to-peak ratio of blood pressure reduction; blood pressure unit: mmHg; n=8)

Experiment 3

Experimental research on the antihypertensive effect of embodiment 1 sample on hereditary hypertensive rats (SHR)

【Experimental Materials】

Rats: hereditary hypertensive rats (SHR), and Wistar rats as normal controls.

Experimental Chinese medicine:

Example 1 Test sample: take 800g of Dendrobium officinale and 250mg of amlodipine besylate in accordance with the 2015 edition of "Chinese Pharmacopoeia". The Dendrobium candidum medicinal material is ground into fine powder, amlodipine besylate powder is added, after mixing, appropriate excipients are added according to pharmaceutical routines to make granules (suspension granules), and dried. For Example 1 sample. Dissolve in hot water before use.

Powder sample of Dendrobium officinale: Take 500g of Dendrobium officinale in accordance with the 2015 edition of "Chinese Pharmacopoeia". The Dendrobium candidum medicinal material is ground into fine powder, and suitable excipients are added according to pharmaceutical routines to make traditional Chinese medicine granules (suspension granules), and dried. Be embodiment 1 sample. Dissolve in hot water before use.

Western antihypertensive control drug: Amlodipine Besylate Tablets (Norvasc), 5mg/tablet, batch number: R78401, produced by Pfizer.

Rat blood pressure measurement: BP-98A non-invasive tail artery blood pressure measuring instrument, Japan Soft Rong Co., Ltd.

【experimental method】

Grouping treatment: All rats were fed adaptively for one week and then measured blood pressure and body weight, and then grouped. SHR rats are divided into embodiment 1 test sample small dosage group (Dendrobium candidum crude drug amount 0.4g/Kg body weight, amlodipine 0.125mg/Kg body weight), embodiment 1 test sample middle dose group (Dendrobium candidum crude drug amount 0.6g /Kg body weight, amlodipine 0.1875mg/Kg body weight), embodiment 1 test sample large dose group (Dendrobium officinale crude drug amount 0.8g/Kg body weight, amlodipine 0.25mg/Kg body weight), Dendrobium officinale powder sample group iron skin Dendrobium crude drug amount 0.8g/Kg body weight, amlodipine group (half amount) amlodipine besylate sheet 0.25mg/Kg body weight, all intragastric administration, hypertension model control group intragastric equivalent normal saline, normal control group The same volume of normal saline was given by intragastric administration. Eight rats in each group were treated for 3 weeks.

Blood pressure measurement: Preheat the rats at 38°C for 5-10 minutes, so as to prevent blood pressure from being measured due to low blood flow in the tail of the rats. The blood pressure of the rat was measured continuously for 3 times in an awake and quiet state, and the average value of the 3 times was taken as the blood pressure value of the rat. Measure blood pressure twice a week, once 2 hours after administration, and once 24 hours after administration, to observe the immediate antihypertensive effect and 24-hour antihypertensive effect (as well as the peak-to-peak ratio of antihypertensive) after administration .

Calculation of valley-to-peak ratio of blood pressure reduction: the difference between blood pressure before treatment - blood pressure 24 hours after administration is the valley value of blood pressure reduction, that is, the lowest point of blood pressure reduction before the next administration; blood pressure before treatment - after administration The difference in blood pressure at 2 hours is the peak value of the blood pressure reduction range, that is, the point at which the blood pressure reduction range is maximum after administration; the valley value of the blood pressure reduction range/the peak value of the blood pressure reduction range = the ratio of the valley-peak reduction range of the blood pressure reduction range. The closer the peak-to-valley ratio of the antihypertensive amplitude is to 1, the more stable the antihypertensive effect is, and the more it meets the requirements of long-acting antihypertensive drugs; the closer the peak-to-valley ratio of the antihypertensive amplitude is to 0, the shorter the antihypertensive effect.

【Experimental Results】

blood pressure data See Table 1 of Experiment 3, Table 2 of Experiment 3, Table 3 of Experiment 3, Table 4 of Experiment 3, and Table 5 of Experiment 3.

Experiment 3: the impact of table 1. embodiment 1 sample on SHR rat administration 2 hours systolic blood pressure ( Unit: mmHg; n=8)

Note: Compared with the model group, △P<0.05▲P<0.01; compared with the amlodipine group and each group ○P<0.05·P<0.01; compared with the Dendrobium officinale powder group and each group☆P<0.05★P<0.01

Experiment 3: the impact of table 2. embodiment 1 sample on SHR rat administration 24 hours systolic blood pressure ( Unit: mmHg; n=8)

Note: Compared with the model group, △P<0.05▲P<0.01; compared with the amlodipine group and each group ○P<0.05·P<0.01; compared with the Dendrobium officinale powder group and each group☆P<0.05★P<0.01

Experiment 3: the impact of table 3. embodiment 1 sample on the diastolic blood pressure of SHR rat administration 2 hours ( Unit: mmHg; n=8)

Note: Compared with the model group, △P<0.05▲P<0.01; compared with the amlodipine group and each group ○P<0.05·P<0.01; compared with the Dendrobium officinale powder group and each group☆P<0.05★P<0.01

Experiment 3: the influence of table 4. embodiment 1 sample on the diastolic blood pressure of SHR rat administration 24 hours ( Unit: mmHg; n=8)

Note: Compared with the model group, △P<0.05▲P<0.01; compared with the amlodipine group and each group ○P<0.05·P<0.01; compared with the Dendrobium officinale powder group and each group☆P<0.05★P<0.01

Experiment 3: Table 5. Embodiment 1 sample is on the influence of the peak-to-peak ratio of systolic blood pressure drop range 24 hours after administration to SHR rats after administration (before treatment-24 hours after administration, the blood pressure drop valley value /Before treatment-the peak value of blood pressure drop 2 hours after administration=the peak-to-peak ratio of blood pressure reduction; blood pressure unit: mmHg; n=8)

1. Blood pressure 2 hours after administration:

Blood pressure at 2 hours after administration during treatment for 3 weeks, blood pressure of embodiment 1 large dose group 172.1 ± 4.66/140.1 ± 4.12mmHg, embodiment 1 middle dose group blood pressure 182.7 ± 5.34/148.6 ± 3.48mmHg, embodiment 1 small dose group blood pressure 188.4±5.39/151.1±4.15mmHg, which were significantly lower than the model group 212.1±6.45/162.1±4.06mmHg (P<0.01 or P<0.05).

The amount of medicine used in the high-dose group of embodiment 1 is the same as that of the western medicine amlodipine group and the Dendrobium officinale powder group, which is the addition of the medicines of the amlodipine group and the Dendrobium officinale powder group, but the dosage of the amlodipine group is 0.25 mg/ Kg body weight is half of the commonly used small dose of 0.5mg/Kg. Embodiment 1 The blood pressure of the high-dose group was significantly lower than the amlodipine group 188.2 ± 4.04/151.3 ± 3.37mHg (P < 0.01), and also lower than the Dendrobium candidum powder group 185.5 ± 4.38/145.8 ± 4.89mHg, and the systolic blood pressure was statistically significant Significant significance (P<0.01). The results showed that after reducing the dosage, the combination of amlodipine and Dendrobium officinale powder can synergistically increase the antihypertensive effect of administration for 2 hours.

2. Blood pressure 24 hours after administration:

Blood pressure at 24 hours after administration during treatment for 3 weeks, blood pressure in embodiment 1 large dose group 187.3 ± 4.24/145.3 ± 5.13mmHg, blood pressure in embodiment 1 middle dose group 189.5 ± 4.15/150.8 ± 4.39mmHg, embodiment 1 small dose group blood pressure 195.8±4.17/154.1±3.68mmHg, which were significantly lower than the model group 209.4±4.65/163.4±5.66mmHg (P<0.01 or P<0.05).

Embodiment 1 The blood pressure of the large dose group was significantly lower than that of the amlodipine group 202.7 ± 6.37/157.3 ± 5.63mHg (P < 0.01/P < 0.05), and also lower than that of the Dendrobium officinale powder group 190.8 ± 4.37/151.3 ± 4.39mHg, but No statistical significance (P>0.05). The results showed that after reducing the dosage, the combination of amlodipine and Dendrobium officinale powder can synergistically increase the antihypertensive effect of administration for 24 hours.

3. After 3 weeks of treatment, the 24-hour blood pressure reduction range/2-hour blood pressure reduction range = blood pressure reduction range valley-peak ratio:

Example 1 High-dose group administration 24 hours blood pressure reduction range 17.0mmHg/administration 2 hours blood pressure reduction range 32.2mmHg = blood pressure reduction range trough-peak ratio 0.53;

Western medicine amlodipine group administration 24 hours blood pressure reduction range 0.8mmHg/administration 2 hours blood pressure reduction range 15.3mmHg = blood pressure reduction range trough-peak ratio 0.05;

Dendrobium candidum powder group administered 24-hour blood pressure reduction range 13.5mmHg/administration 2-hour blood pressure reduction range 18.8mmHg = blood pressure reduction range valley-peak ratio 0.72;

During 3 weeks of treatment, the range of lowering blood pressure of the sample of Example 1 for 24 hours exceeded that of the western medicine amlodipine (half dose) group; Through the combination of Dendrobium candidum powder and western medicine amlodipine (half dose), compared with the dendrobium powder, the 2-hour blood pressure reduction range was increased, and compared with the western medicine amlodipine (half dose), the 24-hour blood pressure reduction was increased. magnitude. Can reduce the dosage of western medicine amlodipine by this combination method.

The rat doses used in this experiment correspond to equivalent adult doses:

Example 1 The corresponding adult dosage of the large dose group administration is about 8 g/day of the crude drug amount of Dendrobium candidum, and 2.5 mg/day of amlodipine;

The dose group administration in Example 1 corresponds to a corresponding adult dose of about 6 g/day of the crude drug amount of Dendrobium officinale, and 1.875 mg/day of amlodipine;

Embodiment 1 Small dose group administration corresponds to a corresponding adult dose of about 4 g/day of crude drug dose of Dendrobium officinale, and 1.25 mg/day of amlodipine;

The corresponding adult dose of Dendrobium candidum powder group is about 8g/day of crude drug dose of Dendrobium candidum;

Western medicine amlodipine group administration corresponds to an equivalent adult dosage of about 2.5 mg/day of amlodipine besylate tablet.

【in conclusion】

2 hours and 24 hours after the administration of the samples in the high-dose, medium-dose and low-dose groups of Example 1, all had significant antihypertensive effects.

Example 1 sample administration 24 hours lower blood pressure range than the western medicine amlodipine (half dose) group; administration 2 hours lower blood pressure range than Dendrobium candidum powder group. Through the combination of Dendrobium candidum powder and western medicine amlodipine (half dose), compared with the dendrobium powder, the 2-hour blood pressure reduction range was increased, and compared with the western medicine amlodipine (half dose), the 24-hour blood pressure reduction was increased. magnitude. Can reduce the dosage of western medicine amlodipine by this combination method.

Claims (2)

1. The application method of Dendrobium officinale and amlodipine in the preparation of medicines for treating hypertension, characterized in that, comprising: taking 8000 parts of Dendrobium officinale and 2.5 parts of amlodipine in parts by weight, and Dendrobium officinale meets the 2015 edition of "Chinese Pharmacopoeia" The requirements in: Dendrobium candidum is ground to powder, add amlodipine powder, and mix; then add excipients according to pharmaceutical routine processing method, make tablet, granule or suspension granule, pill, powder or capsule, obtain Drugs for the treatment of hypertension. 2. The use method of the medicine for treating hypertension prepared in the method described in claim 1, characterized in that, the prepared tablet, granule or suspension granule, pill, powder or capsule is administered orally, and an adult can use it orally. The daily dosage is equivalent to 4g to 8g of the crude drug of Dendrobium officinale, and contains 1.25mg to 2.5mg of amlodipine.