CN107582865A - A kind of pharmaceutical composition and preparation method thereof with fatigue-relieving, strengthen immunity effect - Google Patents
A kind of pharmaceutical composition and preparation method thereof with fatigue-relieving, strengthen immunity effect Download PDFInfo
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- CN107582865A CN107582865A CN201710811981.1A CN201710811981A CN107582865A CN 107582865 A CN107582865 A CN 107582865A CN 201710811981 A CN201710811981 A CN 201710811981A CN 107582865 A CN107582865 A CN 107582865A
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Abstract
The present invention relates to a kind of pharmaceutical composition and preparation method thereof with fatigue-relieving, strengthen immunity effect, belong to technical field of traditional Chinese medicine.The component provided by the invention for including following parts by weight with fatigue-relieving, the pharmaceutical composition of strengthen immunity effect：2.4~3.6 parts of dendrobium candidum, 1.6~2.4 parts of ganoderma lucidum, 0.8~1.2 part of American Ginseng.Pharmaceutical composition provided by the invention can effectively alleviate human-body fatigue, improve body immunity and have no side effect.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicine, and in particular to one kind has fatigue-relieving, strengthen immunity effect
Pharmaceutical composition and preparation method thereof.
Background technology
The mankind further deepen to the understanding of concept of health intension with the development of scientific and technological level, according to the World Health Organization
More than 50 national medical expert, which studies, to be found, the treatment best to various diseases still prevents.The inspection of current various hospitals is only
Caused disease can be diagnosed, once confirming illness, also tended to because finding to feel simply helpless too late, even if passing through
Also difficulty makes patient recover as before to modern medical science means.As long as any disease prevention and maintenance are first, the probability of morbidity will
It is very low, it might even be possible to dissolve completely and avoid.The most important means of prevention disease are enhancing immunity of organisms.
Immunology Today thinks that immunity is human bioequivalence and the physiological reaction for excluding " dissident ", with traditional Chinese medicine
The concept of " healthy tendency " quite communicates part.Traditional medicine thinks：Internal as long as " healthy tendency " is vigorous, it is possible to resists various " perverse trends "
The invasion and attack of (pathogenic factor).Traditional medicine emphasizes the effect of " healthy tendency ", it is believed that generation, development and the conversion of disease both depend on just
The growth and decline of heresy struggle, so as to propose the base therapy thinking of " strengthening vital QI to eliminate pathogenic factors ", that is, emphasize from entirety, strengthen exempting from for body
Epidemic disease power with disease to resist.
It is according to definition of the international movement biochemistry meeting to fatigue：" the physiology course of body, it is impossible to which its function is maintained at one
When specified level or each organ can not maintain its predetermined exercise intensity, it is referred to as fatigue ".The most important physiology essence of fatigue be by
In muscle activity to the influence of energetic supersession function.Due to the increase of energy expenditure when tired, necessarily make the oxygen demand of body
Increase.Now, blood pressure raises, and particularly systolic pressure rise becomes apparent.Moreover, respiration rate increase, pulmonary ventilation volume
Great changes will take place.In a word, Physiological and Biochemical Essence when tired is many, such as accumulation of internal fatigue substances, including breast
Acid, pyruvic acid, hepatic glycogen, the metabolite etc. of nitrogen；Between the imbalance of isohydria, including osmotic pressure, pH value, redox materials
Balance etc..
Fatigue is a comprehensive physiology course for being related to many Physiology and biochemistry factors, is that human body is mental or physical exertion is arrived
A kind of normal physiological phenomena necessarily occurred during certain phase, it had both indicated the temporary transient decline of the original ability to work of body, again
It is probably the tendency that body develops into sick and wounded state.Human-body fatigue and body's immunity decline are closely bound up, both
Although unlike cancer, heart disease, the oncoming force is swift and violent, directly promptly causes death, and it endangers modern's health as one kind
Stealthy killer, had a strong impact on the work and life of people, considerably increased sub-health population quantity, excessive fatigue can add
It is fast dead.When human body is chronically under fatigue state, prematurely senile and burnout syndrome can be produced.
Antifatigue important means is the imbalance supplemented the energy and correct organismic internal environment, particularly humoral system.But
Health medicine of the prior art all leans on " benefit " essentially for the regulation for alleviating human-body fatigue and immunity, that is, supplements various each
Vitamin, the amino acid of sample, it is also effective after taking, but after deactivation very short a period of time, human body still easily feels fatigue, exempts from
Epidemic disease power can decline again, and being not reaching to fundamentally improves human internal organ internal environment, humoral system, supports human body itself each side
The effect of drag is lifted.The health medicine that small part designs with reference to " strengthening vital QI to eliminate pathogenic factors " treatment thoughts, because its compatibility is improper or medicine
Material selection is improper and the adverse reactions such as diarrhoea be present.
The content of the invention
It is an object of the invention to provide a kind of pharmaceutical composition and its system with fatigue-relieving, strengthen immunity effect
Preparation Method.Pharmaceutical composition provided by the invention can effectively alleviate human-body fatigue, improve body immunity and have no side effect.
The invention provides a kind of pharmaceutical composition with fatigue-relieving, strengthen immunity effect, including following weight
The component of part：2.4~3.6 parts of dendrobium candidum, 1.6~2.4 parts of ganoderma lucidum, 0.8~1.2 part of American Ginseng.
Preferably, the weight part ratio of the dendrobium candidum, ganoderma lucidum and American Ginseng is 3：2：1.
Present invention also offers the preparation method of above-mentioned technical proposal described pharmaceutical composition, comprise the following steps：
1) using water as solvent, dendrobium candidum extract solution is prepared, individually or collectively prepares lucidum extracting liquid and American Ginseng extraction
Liquid；
2) extract solution for obtaining the step 1) mixes, and is -0.07~-0.09Mpa in vacuum, temperature is 65 DEG C
It is concentrated under reduced pressure under environment, obtains medicinal material medicinal extract；
3) medicinal extract that the step 2) obtains is spray-dried.
Preferably, the preparation method of the dendrobium candidum extract solution comprises the following steps：
According to weight ratio it is 1 by the dendrobium candidum after crushing and water：(20~30) mix, decoct 1h, be filtrated to get decoction；
According to weight ratio it is 1 by the dregs of a decoction and water：(15~20) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain
To dendrobium candidum extract solution.
Preferably, the preparation method of the lucidum extracting liquid comprises the following steps：
According to weight ratio it is 1 by the ganoderma lucidum after crushing and water：(10~20) mix, decoct 1h, be filtrated to get decoction；
According to weight ratio it is 1 by the dregs of a decoction and water：(10~15) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain
To lucidum extracting liquid.
Preferably, the preparation method of the American Ginseng comprises the following steps：
According to weight ratio it is 1 by the American Ginseng after crushing and water：(10~20) mix, decoct 1h, be filtrated to get decoction；
According to weight ratio it is 1 by the dregs of a decoction and water：(10~15) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain
To American Ginseng extract solution.
Preferably, vacuum is -0.09Mpa in the step 2).
Preferably, the EAT of the spray drying is 170~190 DEG C, and leaving air temp is 90~100 DEG C, is dried
Moisture≤5% afterwards.
Preferably, the EAT is 180 DEG C.
Preferably, 60~80 mesh sieves are crossed after the drying process of the step 3).
The invention provides a kind of pharmaceutical composition with fatigue-relieving, strengthen immunity effect.The present invention passes through choosing
Select and adjust dendrobium candidum, ganoderma lucidum and American Ginseng proportionate relationship so that three realizes preferably collaboration raising effect, i.e., several
After medicinal material combination, its drug effect greatly improves relative to when being used alone, and drug effect is more lasting.Pharmaceutical composition of the present invention
Action pathway include two：External approach, dendrobium candidum, ganoderma lucidum, American Ginseng extract solution are rich in polysaccharide material, saponins material
Etc. various active material, during mixing and being concentrated and dried, intermolecular formation hydrogen bond, while glycosidic bond, ester in moieties
Key or ester bond connect group fracture, form covalent intermolecular bond, lift system stability of the present invention and realize that people is alleviated in collaboration
The effect of body fatigue, strengthen immunity；Internal approach, mainly pass through effect of each component to different target spots in vivo, make phase
Interaction strengthens.Pharmaceutical composition provided by the invention, which can integrate, improves human immunological competence, including improves cellular immunity, body
Liquid immunocompetence, improve NK cytoactives, monocytes/macrophages liver function；Alleviation human-body fatigue, including raising human motion are resistance to
Long ability, improve hepatic glycogen content, reduce Serum lactic acid content and serum urea content.
Brief description of the drawings
Fig. 1 is that the extract solution that the embodiment of the present invention 1 provides extracts schematic flow sheet.
Embodiment
The invention provides a kind of pharmaceutical composition with fatigue-relieving, strengthen immunity effect, including following weight
The component of part：2.4~3.6 parts of dendrobium candidum, 1.6~2.4 parts of ganoderma lucidum, 0.8~1.2 part of American Ginseng.
In the present invention, the weight part ratio of the dendrobium candidum, ganoderma lucidum and American Ginseng is 3：2：1.
The pharmaceutical composition of the present invention includes 2.4~3.6 parts of dendrobium candidum, preferably 3 parts.Iron sheet stone of the present invention
Dry measure used in former times polysaccharide may act on monocytes/macrophages, lymphocyte, human body fluid metabolism etc., can make phagocyte imdex in continuous
Ascendant trend, index and spleen index is improved, improve the activity of the IL-2 and IFN-γ in lymphocyte, strengthen NK cytoactives, reduce
Level of lactic acid, urea nitrogen and MDA etc. acts in blood.The present invention does not have special restriction to the source of the dendrobium candidum,
Using dendrobium candidum commercially available prod well known to those skilled in the art.
The pharmaceutical composition of the present invention includes 1.6~2.4 parts of ganoderma lucidum, preferably 2 parts.Ganoderma lucidum of the present invention can make
For peripheral blood cells, lymphocyte, macrophage, body fluid metabolic etc., peripheral red blood cells, leucocyte and platelet count are improved
Amount, the phagocytic activity of macrophage is improved, stimulates spleen lymphocyte proliferation, transformation, promote delayed allergy effect,
The effect such as Serum lactic acid content, the hepatic glycogen content after lifter motion is reduced, but to thymus gland, spleen without obvious effect.The present invention is right
The source of the ganoderma lucidum does not have special restriction, using the commercially available prod of ganoderma lucidum well known to those skilled in the art.
The pharmaceutical composition of the present invention includes 0.8~1.2 part of American Ginseng, preferably 1 part.American Ginseng of the present invention is lived
Property composition may act on lymphocyte, macrophage, body fluid metabolic etc., rise serum superoxide dismutases activity, improve T leaching
The multiplication capacity of bar cell, improve the effect such as Hemolysin number level.The present invention does not have to the source of the American Ginseng
Special restriction, using conventional American Ginseng commercially available prod well known to those skilled in the art.
In the present invention, dendrobium candidum, ganoderma lucidum, American Ginseng can act on simultaneously with human immunity and tired network of relation
Multiple target spots, the monarch and his subjects mutually help, and realize synergistic enhancing effect.
In the present invention, described pharmaceutical composition preferably includes auxiliary material, and the auxiliary material act as adjusting mouthfeel, described auxiliary
Material is preferably xylitol, and when the auxiliary material is xylitol, the addition of the xylitol is preferably 0.5~1.2 part.The present invention
There is no special restriction to the formulation of the Chinese medicine composition, be using conventional pharmaceutical dosage forms well known to those skilled in the art
Can, such as granule, tablet.
Present invention also offers the preparation method of above-mentioned technical proposal described pharmaceutical composition, comprise the following steps：
1) using water as solvent, dendrobium candidum extract solution is prepared, individually or collectively prepares lucidum extracting liquid and American Ginseng extraction
Liquid；
2) extract solution for obtaining the step 1) mixes, and is -0.07~-0.09Mpa in vacuum, temperature is 65 DEG C
It is concentrated under reduced pressure under environment, obtains medicinal material medicinal extract；
3) medicinal extract that the step 2) obtains is spray-dried.
The present invention prepares dendrobium candidum extract solution, individually or collectively prepares lucidum extracting liquid and American Ginseng using water as solvent
Extract solution.
In the present invention, the preparation method of the dendrobium candidum extract solution comprises the following steps：
According to weight ratio it is 1 by the dendrobium candidum after crushing and water：(20~30) mix, decoct 1h, be filtrated to get decoction；
In the present invention, the dendrobium candidum after the crushing is preferably 10~16 mesh.In the present invention, the dendrobium candidum after the crushing
With water preferably according to weight ratio be 1：25 mixing.
According to weight ratio it is 1 by the dregs of a decoction and water：(15~20) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain
To dendrobium candidum extract solution.In the present invention, the dregs of a decoction are 1 preferably according to weight ratio with water：18.
In the present invention, the preparation method of the lucidum extracting liquid comprises the following steps：
According to weight ratio it is 1 by the ganoderma lucidum after crushing and water：(10~20) mix, decoct 1h, be filtrated to get decoction；At this
In invention, the ganoderma lucidum after the crushing is preferably 10~16 mesh.In the present invention, the ganoderma lucidum after the crushing and water preferably according to
Weight ratio is 1：14 mixing.
According to weight ratio it is 1 by the dregs of a decoction and water：(10~15) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain
To lucidum extracting liquid.In the present invention, the dregs of a decoction are 1 preferably according to weight ratio with water：12.
In the present invention, the preparation method of the American Ginseng comprises the following steps：
According to weight ratio it is 1 by the American Ginseng after crushing and water：(10~20) mix, decoct 1h, be filtrated to get decoction；
In the present invention, the American Ginseng after the crushing is preferably 10~16 mesh.In the present invention, the ganoderma lucidum after the crushing and water are preferred
It is 1 according to weight ratio：14 mixing.
According to weight ratio it is 1 by the dregs of a decoction and water：(10~15) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain
To American Ginseng extract solution.In the present invention, the dregs of a decoction are 1 preferably according to weight ratio with water：12.In the present invention, the spirit
Mixing carries out the extraction of extract solution after sesame and the preferred crushing of American Ginseng.
After obtaining extract solution, the extract solution that the present invention obtains the step 1) mixes, vacuum be -0.07~-
0.09Mpa, temperature are concentrated under reduced pressure in the environment of being 65 DEG C, obtain medicinal material medicinal extract.In the present invention, vacuum in the step 2)
Preferably -0.09Mpa.
After obtaining medicinal extract, the medicinal extract be spray-dried by the present invention.In the present invention, the EAT is excellent
Elect 170~190 DEG C as, leaving air temp is 90~100 DEG C, makes moisture≤5%.In the present invention, the step 3) is entered
Air temperature is preferably 180 DEG C, and leaving air temp is preferably 100 DEG C.
In the present invention, the process of sieving, the preferred mistake 60 of sieving are preferably included after the drying process of the step 3)
~80 mesh, more preferably 70 mesh.
With reference to specific embodiment to a kind of medicine with fatigue-relieving, strengthen immunity effect of the present invention
Composition and preparation method thereof is further described in detail, and technical scheme includes but is not limited to following examples.
Embodiment 1
A kind of to have fatigue-relieving, the pharmaceutical composition of strengthen immunity effect, it is obtained by the following method：
(1) will by weight：3 parts of dendrobium candidum, 2 parts of ganoderma lucidum, 1 part of American Ginseng.Dendrobium candidum is first ground into coarse powder, and 20
Amount decocting is boiled 1 time again, and 15 times of amount decoctings are boiled 2 times, 1 hour every time, filter out decoction, the dregs of a decoction discard, and obtain dendrobium candidum extract solution；
Ganoderma lucidum, American Ginseng are together ground into coarse powder, and 14 times of amount decoctings are boiled 1 time, and 12 times of amount decoctings are boiled 2 times, 1 hour every time, filter out decoction,
The dregs of a decoction discard, and obtain ganoderma lucidum, American Ginseng extract solution.The preparation method flow chart of extract solution is as shown in Figure 1.
(2) after said extracted liquid is well mixed, it is concentrated under reduced pressure under 65 DEG C of environment of -0.09Mpa vacuums, obtains medicinal material
Medicinal extract.
(3) above-mentioned medicinal extract is spray-dried, 180 DEG C of EAT, 100 DEG C of leaving air temp, obtains medicinal material spray powder.
(4) above-mentioned spray powder is crossed into 60 mesh sieves, obtains dendrobium candidum, ganoderma lucidum, American Ginseng mixed extract.Add 0.9 part
Xylitol, the preparation of granule is carried out after mixing.
If above-mentioned mixed extract group (1.2g/kg), dendrobium candidum extract (0.6g/kg) that same procedure obtains, spirit
Sesame extract (0.4g/kg), American ginseng extract (0.2g/kg) and negative control group.Each group gavage is to sample, negative control group
The deionized water of same isometric(al) is given, once a day, continuous 40 days.In experiment the 40th day, cervical dislocation put to death animal.Per mouse
It is sterile to take spleen, it is placed in the small plate for filling appropriate sterile Hanks liquid, is gently torn up with tweezers, is made high cell suspension, 200
Mesh sieve net filtration, wash, count, be finally 2 × 10 with RPMI1640 complete culture solutions adjustment cell concentration7Individual/ml.Before experiment
By YAC-1 cells (target cell) Secondary Culture, washed 3 times with Hanks liquid within 24 hours, adjusted with RPMI1640 complete culture solutions before use
Whole cell concentration is 4 × 105Individual/ml.
Taking target cell and each 100 μ l of splenocyte suspension (effector cell), (effect target is than 50：1), add in 96 well culture plates, target
Cell Spontaneous release hole adds target cell and each 100 μ l of nutrient solution, and target cell maximum release aperture adds target cell and each 100 μ of 1%NP40
l；Above-mentioned items are all provided with three parallel holes, in 37 DEG C, 5%CO2Cultivate 4 hours, centrifuge in incubator, per hole Aspirate supernatant
In the well culture plate of 100 μ l horizontalizations bottom 96, while the μ l of LDH matrix liquids 100 are added, reacted at room temperature 7 minutes, add 1mol/l's per hole
The μ l of hydrochloric acid solution 30, densitometric (OD) value at 490nm wavelength, calculate NK cytoactives.Each group the results are shown in Table 1, experiment knot
Fruit shows：The present invention has collaboration strengthen immunity effect, i.e., strengthen immunity effect effect of the present invention is more than dendrobium candidum, spirit
Sum is answered in sesame, American Ginseng single-action.
If above-mentioned mixed extract group (1.2g/kg), dendrobium candidum extract (0.6g/kg) that same procedure obtains, spirit
Sesame extract (0.4g/kg), American ginseng extract (0.2g/kg) and negative control group.Each group gavage is to sample, negative control group
The deionized water of same isometric(al) is given, once a day, continuous 42 days.After last was to tested material 30 minutes, by root of the tail portion load
The mouse of 5% body weight sheet lead is placed in HSC-100 type constant temperature water bath (depth of water 33cm) went swimming, and control water temperature is at 25 ± 1 DEG C, note
Record mouse from swimming to dead time, i.e. mice burden swimming time.Each group the results are shown in Table 2, and experimental result is shown：This
There is collaboration to alleviate human-body fatigue effect for invention, i.e., human-body fatigue effect effect of the invention of alleviating is more than dendrobium candidum, ganoderma lucidum, west
Sum is answered in American ginseng single-action.
The present invention of table 1 and dendrobium candidum, ganoderma lucidum, American Ginseng list product are to the shadow of NK cells in mice activity and swimming with a load attached to the body ability
Ring
| Group | Number of animals (only) | NK cytoactives (%) | Swimming with a load attached to the body (s) |
| Negative control group | 10 | 18.64±3.94 | 492.4±114.7 |
| Dendrobium candidum extract | 10 | 27.52±4.31 | 606.1±147.0 |
| Ganodenna Lucidum P.E | 10 | 24.68±4.88 | 607.6±124.2 |
| American ginseng extract | 10 | 26.35±5.63 | 614.1±241.1 |
| Mixed extract | 10 | 40.36±7.00 | 742.0±123.3 |
As can be seen from the table, dendrobium candidum, ganoderma lucidum, American Ginseng after processing, there is collaboration to alleviate in above-mentioned health products
The function of human-body fatigue, strengthen immunity.Therefore, the present invention is being ensured each by combining dendrobium candidum, ganoderma lucidum, American Ginseng
On the premise of medical value, unexpected technique effect is generated.
Embodiment 2
On the basis of confirming that dendrobium candidum, ganoderma lucidum, American Ginseng are mutually promoted, the present embodiment is to being concentrated under reduced pressure in preparation
Process has made further research, and step is as follows：
(1) will by weight：2.4 parts of dendrobium candidum, 1.6 parts of ganoderma lucidum, 0.8 part of American Ginseng.Dendrobium candidum is first ground into
Coarse powder, 20 times of amount decoctings are boiled 1 time, and 15 times of amount decoctings are boiled 2 times, 1 hour every time, filter out decoction, the dregs of a decoction discard, and obtain dendrobium candidum
Extract solution；Ganoderma lucidum, American Ginseng are together ground into coarse powder, and 14 times of amount decoctings are boiled 1 time, and 12 times of amount decoctings are boiled 2 times, 1 hour every time, are filtered
Go out decoction, the dregs of a decoction discard, and obtain ganoderma lucidum, American Ginseng extract solution.
(2) after said extracted liquid is well mixed, it is concentrated under reduced pressure under 65 DEG C of environment of vacuum shown in table 2, obtains medicinal material
Medicinal extract.
(3) above-mentioned medicinal extract is spray-dried, 180 DEG C of EAT, 100 DEG C of leaving air temp, obtains medicinal material spray powder.
(4) above-mentioned spray powder is crossed into 60 mesh sieves, obtains dendrobium candidum, ganoderma lucidum, American Ginseng mixed extract.
Negative control group is set up, detects each group NK cells in mice activity and swimming with a load attached to the body ability, institute as described in Example 1
2 must be the results are shown in Table.
The vacuum of table 2 is to dendrobium candidum, the influence of ganoderma lucidum, American Ginseng mixed extract effect
As shown in Table 2, the vacuum being concentrated under reduced pressure has influence largely, preferred scope for the drug effect of health products
For -0.07~-0.09Mpa, under -0.09Mpa, drug effect is best.When less than -0.07Mpa or higher than -0.09Mpa, due to carrying
Functional component decomposition or structure generation change or influence powder yield processed and effect, drug effect in liquid is taken to be suppressed.
Embodiment 3
The present embodiment has made further research to the spray-drying process in preparation, and step is as follows：
(1) will by weight：3.2 parts of dendrobium candidum, 2 parts of ganoderma lucidum, 1.2 parts of American Ginseng.Dendrobium candidum is first ground into slightly
Powder, 20 times of amount decoctings are boiled 1 time, and 15 times of amount decoctings are boiled 2 times, 1 hour every time, filter out decoction, the dregs of a decoction discard, and obtain dendrobium candidum and carry
Take liquid；Ganoderma lucidum, American Ginseng are together ground into coarse powder, and 14 times of amount decoctings are boiled 1 time, and 12 times of amount decoctings are boiled 2 times, 1 hour every time, are filtered out
Decoction, the dregs of a decoction discard, and obtain ganoderma lucidum, American Ginseng extract solution.
(2) after said extracted liquid is well mixed, it is concentrated under reduced pressure under 65 DEG C of environment of -0.09Mpa vacuums, obtains medicinal material
Medicinal extract.
(3) above-mentioned medicinal extract is spray-dried, under EAT shown in table 3,100 DEG C of leaving air temp, obtains medicinal material
Spray powder.
(4) above-mentioned spray powder is crossed into 60 mesh sieves, obtains dendrobium candidum, ganoderma lucidum, American Ginseng mixed extract.
Negative control group is set up, detects each group NK cells in mice activity and swimming with a load attached to the body ability, institute as described in Example 1
3 must be the results are shown in Table.
The EAT of table 3 is to dendrobium candidum, the influence of ganoderma lucidum, American Ginseng mixed extract effect
As shown in Table 3, spray-drying process has influence largely for the drug effect of health products, and preferred scope is
170~190 DEG C.
Embodiment 4
The present embodiment has made further research to dendrobium candidum, ganoderma lucidum, American Ginseng proportions' relation, and step is as follows：
(1) will by weight：Dendrobium candidum, ganoderma lucidum, American Ginseng.Dendrobium candidum is first ground into coarse powder, 20 times of amount decoctings
Boil 1 time, 15 times of amount decoctings are boiled 2 times, 1 hour every time, filter out decoction, the dregs of a decoction discard, and obtain dendrobium candidum extract solution；Ganoderma lucidum, west
American ginseng is together ground into coarse powder, and 14 times of amount decoctings are boiled 1 time, and 12 times of amount decoctings are boiled 2 times, 1 hour every time, filter out decoction, the dregs of a decoction are abandoned
Go, obtain ganoderma lucidum, American Ginseng extract solution.
(2) after said extracted liquid is well mixed, it is concentrated under reduced pressure under 65 DEG C of environment of -0.09Mpa vacuums, obtains medicinal material
Medicinal extract.
(3) above-mentioned medicinal extract is spray-dried, 180 DEG C of EAT, 100 DEG C of leaving air temp, obtains medicinal material spray powder.
(4) above-mentioned spray powder is crossed into 60 mesh sieves, obtains dendrobium candidum, ganoderma lucidum, American Ginseng mixed extract.
(5) above-mentioned mixed extract is mixed 40 minutes for 0.9 part with xylitol, using water as adhesive, boiling granulating, air intake
Temperature 60 C, stop spraying after granularity Detection is qualified, continue to dry to moisture≤5%, obtain granule of the present invention.
By taking granule as an example, according to the technique study dendrobium candidum of embodiment 1, ganoderma lucidum, American Ginseng recipe quantity to potency due,
Three kinds of extracts set up three level values respectively, and experimental factor water-glass is shown in Table 4, and design four factor three horizontal quadrature tests table,
Tested, result is analyzed, experimental result is shown in Table 5, and software analysis is shown according to design table：As a result there was no significant difference
(variance analysis p>0.05), range analysis shows that optimal result is 3 parts of dendrobium candidum, 2 parts of ganoderma lucidum, 1 part of American Ginseng.Experimental result
Show：Collaboration alleviation human-body fatigue strengthen immunity function is respectively provided with the present invention relates to prescription scope and is added using prior art
Auxiliary material is added to carry out preparation.
The orthogonal test factor level table of table 4
Table 5L9(34) Orthogonal Experiment and Design and experimental result table
As can be seen from Table 5：Within the specific limits, dendrobium candidum, ganoderma lucidum, American Ginseng proportions' relation pair health products
Drug effect influence not significantly (p>0.05), i.e., in 2.4~3.6 parts of dendrobium candidum, 1.6~2.4 parts of ganoderma lucidum, American Ginseng 0.8~
In the range of 1.2 parts, health products are respectively provided with collaboration strengthen immunity function, it is preferable that 3 parts of dendrobium candidum, 2 parts of ganoderma lucidum, American Ginseng 1
Part.
Embodiment 5
Pharmaceutical composition is prepared according to the preparation method of embodiment 1, composition detection, inspection are carried out to obtained pharmaceutical composition
It is as shown in table 6 to survey result.
The pharmaceutical composition composition detection table of table 6
The polysaccharide and total saponin content of existing product：
1st, dendrobium officinale American ginseng particle (the strong word G20140312 of state's food), raw material is dendrobium candidum extract, American Ginseng carries
Thing, glucose, maltodextrin are taken, product polysaccharide is 1.6%, saponin(e 0.8%.
2nd, maple standing grain board dendrobium candidum ganoderma lucidum American Ginseng Chinese yam particle (state food strong word G20080107), raw material be dendrobium candidum,
American Ginseng, ganoderma lucidum, Chinese yam, beta-schardinger dextrin, product polysaccharide 0.327%, saponin(e 0.315%.
3rd, Kang Enbei high mountains iron sheetRDendrobium officinale American ginseng particle (the strong word G20120052 of state's food), raw material is iron sheet stone
Dry measure used in former times, American Ginseng, maltitol, product polysaccharide 3%, saponin(e 0.428%
4th, promise board American ginseng-glossy ganoderma-dendrobe particle (the strong word G20110409 of state's food) difficult to understand, raw material is American Ginseng, ganoderma lucidum, dendrobium candidum, paste
Essence, product polysaccharide 1.38%, saponin(e 0.818%.
5th, a famous stem of noble dendrobium ganoderma lucidum is brilliant (the strong word G20060429 of state's food), and raw material is dendrobium candidum, American Ginseng, ganoderma lucidum, sugarcane
Sugar, product polysaccharide 0.253%, saponin(e 0.704%.
Polysaccharide and saponin content in the pharmaceutical composition that the present invention obtains are improved significantly.
Embodiment 6
The acute toxicity test of pharmaceutical composition of the present invention：Press《Health food is examined and assessment technique specification》(2003
Version) acute toxicity test is carried out, the large and small not oral maximal tolerance dose of mouse bisexuality is all higher than 20g/kgbw, is nontoxic level.
Embodiment 7
The micronucleus test of pharmaceutical composition of the present invention：Press《Health food is examined and assessment technique specification》(version in 2003) is entered
Row micronucleus test, if 3 tested material 2.5,5.0,10.0g/kgbw dosage groups, to mouse stomach.Tested material micronucleus test knot
Fruit is feminine gender.
Embodiment 8
The sperm malformation test of pharmaceutical composition of the present invention：Press《Health food is examined and assessment technique specification》(2003
Version) sperm malformation test is carried out, if 3 tested material 2.5,5.0,10.0g/kgbw dosage groups, to male mice gavage.By
Thing sperm malformation test result is tried as feminine gender.
Embodiment 9
The Salmonella reversion test of pharmaceutical composition of the present invention：Press《Health food is examined and assessment technique specification》(version in 2003) is entered
Row Salmonella reversion test, using flat board incorporation methods, proof load 5000,1000,200,40 and 8 μ g/ wares.Tested material Salmonella reversion test knot
Fruit is feminine gender.
Embodiment 10
30 days feeding trials of rat of pharmaceutical composition of the present invention：Press《Health food is examined and assessment technique specification》
(version in 2003) carries out 30 days feeding trials of rat, if 3.33,1.67,0.84g/kg body weight is (equivalent to the 100 of human body recommended amounts
Times, 50 times, 25 times) three dosage groups.Test result indicates that：Under this experiment condition, three dosage group indices are showed no bright
Aobvious toxic reaction.
Embodiment 11
Strengthen immunity functional evaluation
If basic, normal, high three dosage groups (0.16,0.33,1.0g/kg), are respectively equivalent to 5,10, the 30 of people's recommended amounts
Times, while set negative control group.The given the test agent of continuous 30~40 days orally administrations mouse various dose, negative control group is simultaneously
Give drinking pure water.Test result indicates that：This product cellular immune function assay result is positive；Humoral immune function measurement result
It is positive；Monocytes/macrophages functional examination result is positive；NK cytoactive detections result is positive.
Conclusion：The pharmaceutical composition strengthen immunity function of the present invention is strong.
Function of physical fatigue alleviation
If basic, normal, high three dosage groups (0.16,0.33,1.0g/kg), are respectively equivalent to 5,10, the 30 of people's recommended amounts
Times, while set negative control group.The given the test agent of continuous 30~45 days orally administrations mouse various dose, negative control group is simultaneously
Give drinking pure water.Test result indicates that：This product mice burden swimming experimental result is positive；Serum urea measurement result sun
Property；Hepatic glycogen content measurement result is positive；Blood lactase acid measurement result is positive.
Conclusion：The pharmaceutical composition function of physical fatigue alleviation of the present invention is good.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of have fatigue-relieving, the pharmaceutical composition of strengthen immunity effect, it is characterised in that including following parts by weight
Component：2.4~3.6 parts of dendrobium candidum, 1.6~2.4 parts of ganoderma lucidum, 0.8~1.2 part of American Ginseng.
2. pharmaceutical composition according to claim 1, it is characterised in that the weight of the dendrobium candidum, ganoderma lucidum and American Ginseng
Amount part is compared for 3：2：1.
3. based on the preparation method of the described pharmaceutical composition of claim 1 or 2, comprise the following steps：
1) using water as solvent, dendrobium candidum extract solution is prepared, individually or collectively prepares lucidum extracting liquid and American Ginseng extract solution；
2) extract solution for obtaining the step 1) mixes, and is -0.07~-0.09Mpa in vacuum, temperature is 65 DEG C of environment
Under be concentrated under reduced pressure, obtain medicinal material medicinal extract；
3) medicinal extract that the step 2) obtains is spray-dried.
4. preparation method according to claim 3, it is characterised in that the preparation method of the dendrobium candidum extract solution includes
Following steps：
According to weight ratio it is 1 by the dendrobium candidum after crushing and water：(20~30) mix, decoct 1h, be filtrated to get decoction；
According to weight ratio it is 1 by the dregs of a decoction and water：(15~20) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain iron
Skin dendrobium extract.
5. preparation method according to claim 3, it is characterised in that the preparation method of the lucidum extracting liquid includes following
Step：
According to weight ratio it is 1 by the ganoderma lucidum after crushing and water：(10~20) mix, decoct 1h, be filtrated to get decoction；
According to weight ratio it is 1 by the dregs of a decoction and water：(10~15) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain spirit
Sesame extract solution.
6. preparation method according to claim 3, it is characterised in that the preparation method of the American Ginseng includes following step
Suddenly：
According to weight ratio it is 1 by the American Ginseng after crushing and water：(10~20) mix, decoct 1h, be filtrated to get decoction；
According to weight ratio it is 1 by the dregs of a decoction and water：(10~15) mix, decoct 2 times, each 1h, filtering, merge decoction, obtain west
American ginseng extract solution.
7. preparation method according to claim 3, it is characterised in that vacuum is -0.09Mpa in the step 2).
8. preparation method according to claim 3, it is characterised in that the EAT of the spray drying is 170~190
DEG C, leaving air temp is 90~100 DEG C, moisture≤5% after drying.
9. preparation method according to claim 8, it is characterised in that the EAT is 180 DEG C.
10. preparation method according to claim 3, it is characterised in that 60~80 are crossed after the drying process of the step 3)
Mesh sieve.
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Cited By (2)
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|---|---|---|---|---|
| CN108402456A (en) * | 2018-03-22 | 2018-08-17 | 杭州合研科技有限公司 | A kind of health food and preparation method thereof containing dendrobium candidum |
| CN116570685A (en) * | 2023-06-12 | 2023-08-11 | 广东健芝缘保健食品有限公司 | Traditional Chinese medicine composition for nourishing yin, tonifying deficiency and resisting fatigue as well as preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101152007A (en) * | 2007-10-19 | 2008-04-02 | 金华寿仙谷药业有限公司 | A kind of health product composition and preparation method thereof |
| CN102048959A (en) * | 2011-01-14 | 2011-05-11 | 湖南龙石山铁皮石斛基地有限公司 | Dendrobium candidum compound preparation capable of regulating function of human body in two-way manner and preparation method thereof |
| WO2013155717A1 (en) * | 2012-04-20 | 2013-10-24 | 江苏安惠生物科技有限公司 | Body building capsule |
| CN104435749A (en) * | 2014-11-11 | 2015-03-25 | 浙江省医学科学院 | Dendrobium officinale compound preparation as well as preparation method and application of dendrobium officinale compound preparation |
| CN105079491A (en) * | 2015-06-12 | 2015-11-25 | 浙江仙人草保健品有限公司 | Dendrobium officinale and American ginseng capsule and preparation method thereof |
-
2017
- 2017-09-11 CN CN201710811981.1A patent/CN107582865A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101152007A (en) * | 2007-10-19 | 2008-04-02 | 金华寿仙谷药业有限公司 | A kind of health product composition and preparation method thereof |
| CN102048959A (en) * | 2011-01-14 | 2011-05-11 | 湖南龙石山铁皮石斛基地有限公司 | Dendrobium candidum compound preparation capable of regulating function of human body in two-way manner and preparation method thereof |
| WO2013155717A1 (en) * | 2012-04-20 | 2013-10-24 | 江苏安惠生物科技有限公司 | Body building capsule |
| CN104435749A (en) * | 2014-11-11 | 2015-03-25 | 浙江省医学科学院 | Dendrobium officinale compound preparation as well as preparation method and application of dendrobium officinale compound preparation |
| CN105079491A (en) * | 2015-06-12 | 2015-11-25 | 浙江仙人草保健品有限公司 | Dendrobium officinale and American ginseng capsule and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李莉，徐建华，范允坤，陈立钻: "石斛洋参冲剂的药理作用研究", 《中成药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108402456A (en) * | 2018-03-22 | 2018-08-17 | 杭州合研科技有限公司 | A kind of health food and preparation method thereof containing dendrobium candidum |
| CN116570685A (en) * | 2023-06-12 | 2023-08-11 | 广东健芝缘保健食品有限公司 | Traditional Chinese medicine composition for nourishing yin, tonifying deficiency and resisting fatigue as well as preparation method and application thereof |
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Application publication date: 20180116 |