CN107549207B - A Bemisia tabaci compound essential oil repellent and special greenhouse entrance high-pressure micro-mist curtain equipment - Google Patents

Abstract

The invention discloses a bemisia tabaci composite essential oil repellent agent and special greenhouse entrance high-pressure micro-fog door curtain equipment. The bemisia tabaci compound essential oil repellent comprises the following components in parts by volume: 1-5 parts of artemisia capillaris oil, 1-5 parts of hyssop oil, 1-5 parts of broadleaf thyme oil, 1-5 parts of rue oil, 1-5 parts of basil oil and 1-5 parts of medicinal sage oil. The essential oil repellent has the functions of repelling pests with lasting release, good volatilization stability, no harm to the environment and no pollution to air, can be used at low dosage, particularly can efficiently repel the global invasive pest bemisia tabaci, effectively performs comprehensive control on greenhouse pests such as bemisia tabaci and the like, efficiently reduces the greenhouse pests to damage greenhouse crops, and reduces the agricultural economic loss.

Description

A Bemisia tabaci compound essential oil repellent and a special high-pressure micro-mist door curtain at the entrance of a greenhouse equipment

technical field

The invention relates to the field of comprehensive insect control, in particular to a Bemisia tabaci compound essential oil repellent and a special greenhouse entrance high-pressure micro-mist door curtain device.

Background technique

The invention relates to the field of integrated insect management, in particular to a greenhouse entrance high-pressure micro-mist door curtain device for repelling pests, especially the global invasive pest Bemisia tabaci.

Aromatic Plants (Aromatic Plants) is a new type of economic crops. Essential Oils (Eos) are extracted from different plant tissues such as roots, stems, leaves, flowers, fruits, seeds, branches or secretions. A general term for volatile oily liquid substances and (or scented) substances. Usually, plant essential oils are used to prepare flavors and fragrances, but in recent decades, studies have found that some aromatic plants, essential oils and their components have high efficiency against agricultural pests, attract natural enemies, and have insecticidal effects on environmental safety. Insects have different degrees of ecological management. The biological activities of aromatic volatile substances on insects mainly include fumigation, contact killing, attracting, repelling, antifeeding and growth inhibition. People pay more and more attention to the potential harm to the environment, food safety and health caused directly or indirectly by the application of toxic chemicals. Compared with traditional chemical pesticides, the development of high-efficiency, low-toxicity, low-residue, and environmentally-compatible ecological management agents that conform to the concepts of environmental protection, health and sustainable development has become the theme of today's agricultural development research. An efficient and safe natural ecological management agent, and the application prospect for agricultural insects is very broad.

The use of single essential oils has been extremely common, and the use of compound essential oils has a long history. It is generally believed that essential oils can improve the overall function when used in combination, and the effects brought by the combination are also divided into synergistic, additive, irrelevant and antagonism in four situations.

The CN200510087369.1 invention discloses an automatic nebulizer system comprising a control unit having an electronic circuit adapted to activate the nebulizer for a predetermined time interval in response to detected ambient light conditions of expected intensity and duration. The system may further include a power source and a plurality of spaced apart nebulizer heads. The preferred utility of the system of the invention is to spray a dilute solution of insecticide or repellent during periods of significant insect activity that typically occurs around dusk and dawn. The invention also provides a manual override and remote receiving unit for use of the system outside of dusk and dawn.

CN201610365633.1 The invention discloses a preparation method of a plant-derived insect repellent in the technical field of pesticide production, which is mainly composed of a tomato plant extract, a coriander plant extract, and a surfactant OP. The main active components of the tomato extract are It is n-hexanol and (Z)-3-acetate leaf alcohol ester; the active ingredients of the coriander extract are n-nonanal and trans-2-hexenal, and the preparation method is to synthesize the tomato plant extract, the coriander plant The extraction liquid and the surfactant OP are mixed in proportion, and then an appropriate amount of water is added to mix and stir to form a solvent. The insect repellent has the characteristics of green environmental protection, no residue, easy degradation, low cost, convenient use and the like, and the repelling effect on moth larvae, aphids and the like can reach more than 85%.

CN201580001818.8 The invention discloses an easy-to-use insect repellent and insect repellent with high repellent effect on harmful insects, excellent sustainability, lower toxicity to humans, animals and crops, and no soil pollution. avoidance method. A combination of at least one compound selected from cyclic monoterpene alcohols and/or a compound selected from cyclic monoterpene ketones and a compound selected from cyclic sesquiterpenes as active ingredients , directly, or infiltrated into an appropriate carrier, or mixed with a carrier and adding appropriate additives to make solids, oils (liquids), emulsions, hydrations, pastes, gels, aerosols, micro- Repellents in dosage forms such as capsules can be used by any method of spraying, spreading, coating, volatilizing, or smoking.

CN201110418944.7 The invention discloses the application of borneol acetal-1,3-propanediol in insect repellent and antifeedant, including cockroaches, ants and red grain thieves. The borneol acetal-1,3-propanediol is dissolved in an organic solvent such as ethanol or acetone and used directly, or used in combination with other equipment for repelling cockroaches and ants. The purpose of using the insect repellent in the present invention is to protect rather than kill, and has the advantages of being safe to humans and animals, and being environmentally friendly. Borneol acetal-1,3-propanediol has good repellent activity against cockroaches, ants and red grain thieves, and is of great significance for the control of insects.

CN201010219927.6 The invention discloses that a hydroxycitronellal 1,2-propanediol acetal is used as a repellant, and the compound hydroxycitronellal 1,2-propanediol acetal shown below is used as an insect repellant. The hydroxycitronellal 1,2-propanediol acetal described in the invention can be used as a substitute for DEET for repelling Culex pipiens pipiens.

CN200810019316.X The invention discloses the application of hydroxycitronellal propionate as insect repellent and antifeedant, which includes the application of hydroxycitronellal propionate as repellent for cockroaches and red grain thieves, Application of hydroxycitronellal propionate as antifeedant for diamondback moth larvae. The results of bioassays showed that the repellent activity against German cockroach was about 63%; the repellent activity against red grain thief was over 90%; the antifeeding activity against diamondback moth larvae was over 67%.

Studies have shown that the application research of aromatic plants and their essential oils in pest control mainly focuses on stored grain pests and sanitary pests. There are relatively few reports on field and greenhouse crop pests, and most of them are fumigation and contact killing. The systematic research on repelling and attracting activities that affect the life behavior of adults has only gradually increased in recent years. The research on the behavioral selection of Bemisia tabaci by aromatic volatile substances is mostly based on the volatile components of crops such as wild tomato and celery. Field experiments were carried out in the intercropping method, and essential oils with low dosage, high efficiency, environmental protection and safety can be obtained by ordinary steam distillation. The source is simple, and the cost of extracting components is extremely low. There are relatively few applications for integrated control of Bemisia tabaci.

SUMMARY OF THE INVENTION

In order to solve the above problems, the present invention provides a Bemisia tabaci compound essential oil repellent and a special greenhouse entrance high-pressure micro-mist door curtain device.

First, the present invention provides a Bemisia tabaci compound essential oil repellent, which, in parts by volume, comprises the following components: 1-5 parts of Artemisia chinensis oil, 1-5 parts of herb oil, 1-5 parts of broad-leaved thyme oil 5 parts, 1-5 parts rue oil, 1-5 parts basil oil, 1-5 parts medicinal sage oil.

In one embodiment of the present invention, the compound essential oil repellent includes the following components in parts by volume: 5 parts of Artemisia annua oil, 4 parts of Herb Oil, 3 parts of broad-leaved thyme oil, and 3 parts of rue oil , 4 parts of basil oil, 3 parts of medicinal sage oil.

In another embodiment of the present invention, the compound essential oil repellent includes the following components in parts by volume: 4 parts of Artemisia annua oil, 4 parts of Herb Oil, 2 parts of broadleaf thyme oil, 2 parts of rue oil parts, 2 parts basil oil, 2 parts medicinal sage oil.

In another embodiment of the present invention, the complex essential oil repellent further comprises ethanol and an emulsifier.

Wherein, the ethanol content is 5% of the total volume, and the emulsifier content is 3% of the total volume.

The present invention also provides a method for preparing the essential oil repellent, which comprises the steps of uniformly mixing the essential oil of Artemisia vulgaris, the essential oil of Herbaceae, the essential oil of broadleaf thyme, the essential oil of rue, the essential oil of basil and the essential oil of medicinal sage according to the proportion, and then adding 5% ethanol and 3% emulsifier, ultrasonically vibrated for 5-15s, to obtain a compound essential oil repellent.

The essential oil of Artemisia absinthium used in the present invention belongs to the genus Asteraceae, and the whole plant is extracted from the ground at the full flowering stage. The development of repellent for Bemisia tabaci has not yet been reported. Its main aromatic volatile components are myrcene, thujone, juniper acetate and so on.

The essential oil of Hyssopus officinalis used in the present invention belongs to the genus Hyssopus officinalis. It is extracted from the whole plant on the ground at the full flowering stage, and the essential oil is pale yellow. The development of whitefly repellent has not been reported yet. The main aromatic volatile components are pinone, pinene, borneol, geraniol, thujone, camphene, limonene, phellandrene, etc.

The broad-leaved thyme (Thymus pulegoides) essential oil used in the present invention belongs to the genus Lamiaceae, and the whole plant is extracted from the ground. The development of whitefly repellent has not been reported yet. Its main aromatic volatile components are thymol, cymene, bisabolene, methyl thymol blue and so on.

The ruta (Ruta graveolens) essential oil used in the present invention belongs to the Rutaceae genus. It is extracted from the whole plant on the ground at the full flowering stage, and the essential oil is light yellow. Silverfish repellent, and the research and development of Bemisia tabaci repellent has not yet been reported. Its main aromatic volatile components are 2-nonanone, 2-undecanone, 1-nonene and so on.

The basil (Ocimum basilicum) essential oil used in the present invention belongs to the genus Lamiaceae. It is extracted from the whole plant on the ground at the full flowering stage, and the essential oil is pale yellow. However, the research and development of repellents for Bemisia tabaci have not been reported. Its main aromatic volatile components are basilene, T-pinene, 1,8 cineole, linalool, geraniol, limonene, 3-carene, methyl piperonol, eugenol, fennel ether, methyl cinnamate, hexen-3-ol-1, 3-octanone, and uronic acid, eugenol methyl ether, etc.

The medicinal sage (Salvia officinalis) essential oil used in the present invention belongs to the genus Salvia officinalis, the whole plant is extracted from the ground, the essential oil is pale yellow, and the behavioral selection response to Bemisia tabaci is extremely significant. And the research and development of whitefly repellent for Bemisia tabaci has not been reported yet. Its main aromatic volatile components are 1,8 eucalyptol, thujone, elemene and so on.

The extraction techniques of various essential oils of the present invention are all conventional technical means in the art.

The present invention also provides a greenhouse entrance high-pressure micro-mist door curtain device specially used for the Bemisia tabaci compound essential oil repellent, including a spray device, a liquid storage container and transportation accessories, and the spray device includes a high-pressure and high-efficiency micro-mist and a fine mist nozzles, a plurality of fine mist nozzles are arranged at a certain distance and fixed above the greenhouse door frame; the liquid storage container includes a repellent carrying box, a repellent dilution water tank and a fixed frame; the transportation accessories include a water source filter , PU pipe and fixed parts are connected with the water source filter, liquid storage container and spray device through the PU pipe.

In one embodiment of the present invention, the high-voltage micro-mist door curtain device for greenhouse entrance special for Bemisia tabaci compound essential oil repellent further has an electronic circuit, and the electronic circuit is adapted to respond to the detected entry and exit of people, that is, the entrance door of the greenhouse opens. If the conditions are met, the nebulizer is activated for a predetermined period of time of 0.5 to 1 min. Before the system equipment is turned on, the repellent carrier box is filled with the prepared repellent, turn on the switch of the repellent carrier box, use a scale of repellant at a time, turn on the water source switch, and the repellant and water are sufficient. Mix evenly to fill the entire water tank, start the set high-pressure and high-efficiency micro-mist system, and spray the fine mist nozzle regularly.

In one embodiment of the present invention, an induction spraying procedure is set for personnel entering and exiting the greenhouse entrance, that is, once the greenhouse entrance is opened, the micro-mist is automatically sprayed to prevent the winged species, especially harmful insects such as Bemisia tabaci from being sprayed with the opening of the greenhouse entrance. Migrates, lands and reproduces rapidly to harm greenhouse crops.

The essential oil repellent of the present invention has the pest repelling functions of long-lasting release, good volatility stability, no harm to the environment and no air pollution, and can be used in a low dose, and is especially effective for repelling the global invasive pest Bemisia tabaci , Effectively carry out comprehensive control of greenhouse pests such as Bemisia tabaci, effectively reduce the damage of greenhouse pests to greenhouse crops, and reduce agricultural economic losses.

With the rapid development of facility agriculture, the occurrence of Bemisia tabaci has become more and more rampant, which has become one of the main constraints affecting the sustainable development of facility agriculture. Bemisia tabaci are seriously infested, and they are basically distributed on the back of crop leaves and are not suitable for large-scale spraying. Insecticide, the present invention or used to consolidate the control effect after concentrated killing, will be an ideal technical measure, and will play a positive role in alleviating problems such as environmental pollution and drug resistance. And research in recent decades has found that essential oils and single components have strong properties of inhibiting or killing microorganisms such as bacteria and fungi. In addition to insect pests, the probability of occurrence of greenhouse diseases is also reduced to a certain extent.

Description of drawings

Fig. 1 shows the schematic diagram of the high-pressure micro-mist door curtain equipment at the entrance of the greenhouse specially used for the Bemisia tabaci compound essential oil repellent of the present invention.

In the figure, 1 is a high-pressure and high-efficiency micro-mist, 2 is a repellent dilution water tank, 3 is a diluent filter, 4 is a repellent carrier box, 5 is a fine mist nozzle, and 6 is the greenhouse entrance.

Detailed ways

The following examples are intended to illustrate the present invention, but not to limit the scope of the present invention.

In conjunction with accompanying drawing 1, the greenhouse entrance high pressure micro-mist door curtain equipment specially used for the Bemisia tabaci compound essential oil repellent of the present invention will be described in detail.

A high-pressure micro-mist door curtain device for greenhouse entrance special for the described Bemisia tabaci compound essential oil repellent, including a spray device, a liquid storage container and a transportation accessory, and the spray device includes a high-pressure and high-efficiency micro-mist device 1 and a fine mist nozzle 5 , a plurality of fine mist nozzles 5 are arranged at a certain distance and fixed on the top of the greenhouse door frame; the liquid storage container includes a repellent carrying box 4, a repellent dilution water tank 2 and a fixed frame; the transportation accessories include a water source filter The water source filter 3, the liquid storage container and the spray device are connected through the PU pipe.

The greenhouse entrance high-pressure micro-mist door curtain device specially used for the Bemisia tabaci compound essential oil repellent also has an electronic circuit, which is suitable for activating the sprayer for a predetermined period in response to the detected entry and exit of persons, that is, the opening and closing conditions of the entrance door of the greenhouse. Time 0.5 ~ 1min.

Before the system equipment is turned on, the repellent carrier box is filled with the prepared repellent, turn on the switch of the repellent carrier box, use a scale of repellant at a time, turn on the water source switch, and the repellant and water are sufficient. Mix evenly to fill the entire water tank, start the set high-pressure and high-efficiency micro-mist system, and spray the fine mist nozzle regularly.

Set up induction spraying procedures for personnel entering and leaving the greenhouse entrance, that is, once the greenhouse entrance is opened, it will be automatically sprayed to avoid micro-mist, so as to prevent winged species, especially harmful insects such as Bemisia tabaci, from migrating, descending and rapidly multiplying with the opening of the greenhouse entrance. Greenhouse crops.

Example 1

The traditional behavioral response measurement is an important method to study the relationship between insects and plants. The Y-type olfactometer is the most commonly used classical method to determine the behavior of insects. A blank control group and a volatile odor source group were set up, and the behavioral choices of each insect on the two arms were observed and counted. Based on this, the above 6 kinds of aromatic plant essential oils used in the present invention were comprehensively screened out from the results of further compound research on 14 kinds of single-party effective essential oils from 46 kinds of aromatic plant essential oils unilaterally on Bemisia tabaci behavior selection biological activity measurement results. The species with extremely strong repellent effect and high plant essential oil extraction rate (Table 1), it can be seen that these 6 kinds of aromatic plant essential oils have the potential to be developed into composite repellents for controlling greenhouse pests, especially for Bemisia tabaci, and the mixture of essential oils The proportioning use has a certain synergistic effect.

Table 1 Determination of biological activities of six aromatic plant essential oils on Bemisia tabaci behavioral selection

essential oil Air(%) Odour (%) Central Asian Artemisia 83.33*** 16.67 god vanilla 83.33*** 16.67 broadleaf thyme 78.89*** 21.11 rue 76.67*** 23.33 basil 76.67*** 23.33 medicinal sage 70.00*** 30.00

Note: ***, means P<0.001 in the χ ² test.

Example 2

Take 5 parts of Artemisia annua oil, 4 parts of herb oil, 3 parts of broadleaf thyme oil, 3 parts of rue oil, 4 parts of basil oil, and 3 parts of medicinal sage oil, mix well in a glass or stainless steel container, Add 5% ethanol (95%) and 3% emulsifier, and ultrasonically vibrate for 10s to obtain a mixed essential oil repellent, which is stored in a refrigerator at 4° C. for later use. The test method is the same as that of Example 1, and the results of its biological activity measurement on Bemisia tabaci behavior selection are shown in Table 2.

The compound essential oil repellent is placed in the repellent carrier box to be activated, and the amount used each time is one scale of the carrier box.

Table 2 Determination results of biological activity of compound essential oil repellent on Bemisia tabaci behavior selection

testing object No odour (%) Odour (%) Example 2 83.33*** 16.67

Note: ***, means P<0.001 in the χ ² test.

As can be seen from the above table, the compound essential oil repellent of the implementation case 2 has a significant repelling effect on Bemisia tabaci, and its effect is equivalent to the most effective result in the single prescription, and the use of the compound prescription can also comprehensively treat different oil production. The reconciliation of essential oils obtained from aromatic plants at a high rate avoids the problem of higher single cost of finished products, and the use of compound essential oils can also reduce potential insect resistance.

Example 3

Take 4 parts of Artemisia annua oil, 4 parts of herb oil, 2 parts of broadleaf thyme oil, 2 parts of rue oil, 2 parts of basil oil, 2 parts of medicinal sage oil, mix well in a glass or stainless steel container, Add 5% ethanol (95%) and 3% emulsifier, and ultrasonically vibrate for 10s to obtain a mixed essential oil repellent, which is stored in a refrigerator at 4° C. for later use. The test method is the same as that of Example 1, and the results of its biological activity assay on Bemisia tabaci behavior selection are shown in Table 3.

The compound essential oil repellent is placed in the repellent carrier box to be activated, and the amount used each time is one scale of the carrier box.

Table 3 Determination results of biological activity of compound essential oil repellent on Bemisia tabaci behavior selection

testing object No odour (%) Odour (%) Example 2 82.22*** 17.78

Note: ***, means P<0.001 in the χ ² test.

As can be seen from the above table, the compound essential oil repellent of embodiment 3 has significant repelling effect to Bemisia tabaci, and its effect is almost equivalent to the most effective result in the single prescription, and the use of the compound prescription can also comprehensively treat different The blending of essential oils obtained from aromatic plants with high oil yield avoids the problem of higher single cost of finished products, and the use of compound essential oils can also reduce potential insect resistance.

Example 4

Take 3 parts of Artemisia annua oil, 5 parts of Herb Oil, 3 parts of broadleaf thyme oil, 2 parts of rue oil, 1 part of basil oil, 2 parts of medicinal sage oil, mix well in a glass or stainless steel container, Add 5% ethanol (95%) and 3% emulsifier, and ultrasonically vibrate for 10s to obtain a mixed essential oil repellent, which is stored in a refrigerator at 4° C. for later use. The test method is the same as that of Example 1, and the results of its biological activity measurement on Bemisia tabaci behavior selection are shown in Table 4.

The compound essential oil repellent is placed in the repellent carrier box to be activated, and the amount used each time is one scale of the carrier box.

Table 4 Determination results of biological activity of compound essential oil repellent on Bemisia tabaci behavior selection

testing object No odour (%) Odour (%) Example 3 78.89*** 21.11

Note: ***, means P<0.001 in the χ ² test.

As can be seen from the above table, the compound essential oil repellent of embodiment 4 has significant repelling effect to Bemisia tabaci, and its effect is comparable with the more effective results in the single prescription, and the use of the compound prescription can also be comprehensively used for different oil production. The reconciliation of essential oils obtained from aromatic plants at a high rate avoids the problem of higher single cost of finished products, and the use of compound essential oils can also reduce potential insect resistance.

Example 5

Take 2 parts of Artemisia annua oil, 2 parts of herb oil, 2 parts of broadleaf thyme oil, 4 parts of rue oil, 5 parts of basil oil, 5 parts of medicinal sage oil, mix well in a glass or stainless steel container, Add 5% ethanol (95%) and 3% emulsifier, and ultrasonically vibrate for 10s to obtain a mixed essential oil repellent, which is stored in a refrigerator at 4° C. for later use. The test method is the same as that of Example 1, and the results of its biological activity measurement on Bemisia tabaci behavior selection are shown in Table 5.

The compound essential oil repellent is placed in the repellent carrier box to be activated, and the amount used each time is one scale of the carrier box.

Table 5 Determination results of biological activity of compound essential oil repellent on Bemisia tabaci behavior selection

testing object No odour (%) Odour (%) Example 4 76.67*** 23.33

Note: ***, means P<0.001 in the χ ² test.

As can be seen from the above table, the compound essential oil repellent of embodiment 5 has significant repelling effect to Bemisia tabaci, and its effect is comparable with the more effective results in the single prescription, and the use of the compound prescription can also be comprehensively used for different oil production. The reconciliation of essential oils obtained from aromatic plants at a high rate avoids the problem of higher single cost of finished products, and the use of compound essential oils can also reduce potential insect resistance.

The above are only the preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, without departing from the technical principles of the present invention, several improvements and modifications can be made. These improvements and modifications It should also be regarded as the protection scope of the present invention.

Claims (4)

1. A Bemisia tabaci compound essential oil repellent, by volume, comprising the following components: 1) 5 parts of essential oil of Artemisia chinensis, 4 parts of essential oil of Herba Radix, 3 parts of essential oil of broadleaf thyme, 3 parts of essential oil of rue, 4 parts of essential oil of basil, and 3 parts of essential oil of medicinal sage; or 2) 4 parts of Artemisia annua essential oil, 4 parts of Herbs essential oil, 2 parts of broadleaf thyme essential oil, 2 parts of rue essential oil, 2 parts of basil essential oil, 2 parts of medicinal sage essential oil. 2. The whitefly compound essential oil repellent of claim 1, further comprising ethanol and an emulsifier. 3. The whitefly compound essential oil repellent according to claim 2, wherein the ethanol content is 5% of the total volume, and the emulsifier content is 3% of the total volume. 4. the preparation method of the Bemisia tabaci compound essential oil repellent described in any one of claim 1-3, it is characterised in that it is by proportioning Artemisia spp. Essential oil, basil essential oil, and medicinal sage essential oil are mixed evenly, 5% ethanol and 3% emulsifier are added, and ultrasonically oscillated for 5-15s to obtain a compound essential oil repellent.

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