CN107490656B - Test device and using method thereof - Google Patents
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- CN107490656B CN107490656B CN201710624774.5A CN201710624774A CN107490656B CN 107490656 B CN107490656 B CN 107490656B CN 201710624774 A CN201710624774 A CN 201710624774A CN 107490656 B CN107490656 B CN 107490656B
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Abstract
The invention provides a testing device and a using method thereof, wherein the device comprises: the device comprises a cosolvent quantitative filling unit (1), a solvent storage tank (2), a solvent quantitative filling unit (3), an automatic gradient equivalent step-by-step liquid medicine preparation unit (4), an experimental sample mother liquid quantitative filling unit (5), a linear electric cylinder slider (6) arranged on a linear electric cylinder track (12), a plurality of experimental sample dilution mixing cylinders (7) numbered in sequence, a plurality of experimental sample storage cylinders (8) numbered in sequence, a plurality of liquid feeding collecting ports (10) arranged on the experimental sample dilution mixing cylinders (7) and the last experimental sample storage cylinders, a plurality of aquatic organism experimental cylinder units (9) numbered in sequence and a drainage collecting tank (11) arranged at the bottom of the aquatic organism experimental cylinder units (9); wherein, the number of the experimental sample diluting and mixing cylinder (7), the experimental sample storage cylinder (8) and the aquatic organism experimental cylinder unit (9) is consistent. The test device can realize automatic dilution of the experimental sample, automatic filling equal-quantity periodic update of the experimental sample solution and running water test of the reproductive toxicity of the large aquatic organisms.
Description
Technical Field
The invention relates to the technical field of chemistry, in particular to a testing device and a using method thereof.
Background
In the chemical field, some tests for biological characteristics are usually performed, for example, when the growth state of aquatic organisms (such as daphnia) is tested in the test cups, an equal amount of liquid medicine needs to be put into each test cup, the liquid medicine in each test cup needs to be synchronously updated in an equal amount, and the growth state of the aquatic organisms in each test cup is observed.
Therefore, a flow water type daphnia magna breeding toxicity tester is needed to complete the preparation of an automatic gradient equivalent step-by-step experimental sample, the automatic dilution of the experimental sample and the automatic filling equivalent periodic update of the experimental sample solution, so that the flow water test of daphnia magna breeding toxicity is realized.
Disclosure of Invention
The embodiment of the invention provides a testing device and a using method thereof, which are used for solving the problems of completing the preparation of an automatic gradient equal-quantity step-by-step concentration experimental sample, the automatic dilution of the experimental sample and the automatic filling equal-quantity periodic updating of an experimental sample solution, thereby realizing the flowing water test of the reproductive toxicity of daphnia magna.
An embodiment of the present invention provides a test apparatus, including: the device comprises a cosolvent quantitative filling unit 1, a solvent storage tank 2, a solvent quantitative filling unit 3, an automatic gradient equivalent step-by-step liquid medicine preparation unit 4, an experimental sample mother liquid quantitative filling unit 5, a linear electric cylinder slide block 6 arranged on a linear electric cylinder track 12, a plurality of sequentially numbered experimental sample diluting and mixing cylinders 7, a plurality of sequentially numbered experimental sample storage cylinders 8, a plurality of liquid feeding collecting ports 10 arranged on the plurality of experimental sample diluting and mixing cylinders 7 and the last experimental sample storage cylinders, a plurality of sequentially numbered aquatic organism experimental cylinder units 9 and a drainage collecting tank 11 arranged at the bottom of the aquatic organism experimental cylinder units 9; wherein the numbers of the experimental sample diluting and mixing cylinder 7, the experimental sample storage cylinder 8 and the aquatic organism experimental cylinder unit 9 are consistent.
In a possible embodiment, the cosolvent quantitative filling unit 1 is communicated with the liquid adding collecting port 10 of the first experimental sample diluting and mixing cylinder 7 of the plurality of sequentially numbered experimental sample diluting and mixing cylinders 7 through a hose, wherein the experimental sample diluting and mixing cylinder 7 is provided with the liquid adding collecting port 10 corresponding to the experimental sample diluting and mixing cylinder.
In a possible embodiment, the automatic gradient and equivalent stepwise liquid medicine preparation unit 4 and the quantitative filling unit 5 of the experimental sample mother liquid are communicated with the liquid filling collecting ports 10 of the last experimental sample diluting and mixing cylinders 7 of the plurality of sequentially numbered experimental sample diluting and mixing cylinders 7 through hoses.
In a possible embodiment, the solvent storage tank 2, the solvent quantitative filling unit 3 and the automatic gradient equivalent step-by-step liquid medicine preparation unit 4 are communicated through hoses; the hose of the solvent quantitative filling unit 3 and the hose of the automatic gradient equivalent gradual liquid medicine preparation unit 4 are fixed to the linear electric cylinder slider 6, so that liquid medicines in the solvent quantitative filling unit 3 and the automatic gradient equivalent gradual liquid medicine preparation unit 4 flow into the experimental sample dilution mixing cylinder 7 through the sliding of the linear electric cylinder slider 6.
In a possible embodiment, a hose is provided at the bottom of the set portion of the plurality of sequentially numbered test sample dilution and mixing cylinders 7 and a hose valve 13 is provided on the hose, wherein the set portion includes a portion excluding the last one of the plurality of sequentially numbered test sample dilution and mixing cylinders 7; the bottom of the experimental sample dilution and mixing cylinder 7 is provided with a magnetic stirrer 14.
In a possible embodiment, the flexible tube disposed in the experimental sample dilution mixing cylinder 7 is communicated with the experimental sample storage cylinder 8 with corresponding number, and the last experimental sample storage cylinder 8 of the plurality of experimental sample dilution mixing cylinders 8 with sequential number is provided with a liquid feeding collection port 10 corresponding to the last experimental sample storage cylinder 8.
In a possible embodiment, the bottom of the experimental sample storage cylinder 8 is provided with a hose and a hose valve 13 is provided on the hose, and the bottom of the experimental sample storage cylinder 8 is provided with a magnetic stirrer 14.
In a possible embodiment, the hoses disposed in the experimental sample storage cylinders 8 are communicated with the corresponding numbers of the aquatic organism experimental cylinder units 9.
In a possible embodiment, the bottom of the aquatic organism experimental tank unit 9 is provided with a drain pipe, and the drain pipe is communicated with the drain collecting tank 11.
The embodiment of the invention also provides a using method of the testing device, which comprises the following steps: a cosolvent quantitative filling unit is used for injecting a cosolvent mother solution into a liquid filling collecting port of a first experimental sample diluting and mixing cylinder in a plurality of sequentially numbered experimental sample diluting and mixing cylinders through a hose; the experimental sample mother liquor quantitative filling unit is used for filling the experimental sample mother liquor into liquid adding collecting openings of last experimental sample dilution mixing cylinders in the plurality of sequentially numbered experimental sample dilution mixing cylinders through hoses; the solvent quantitative filling unit extracts the solvent from the solvent storage tank, moves through the linear electric cylinder, and sequentially injects the solvent into the plurality of test sample dilution mixing cylinders which are sequentially numbered, and simultaneously injects the solvent into the last test sample storage cylinder in the plurality of test sample dilution mixing cylinders which are sequentially numbered; the automatic gradient equivalent step-by-step liquid medicine preparation unit extracts a solvent from a solvent storage tank and extracts a primary diluted mother liquid from the last experimental sample dilution mixing cylinder in the plurality of sequentially numbered experimental sample dilution mixing cylinders for mixing; and then mix the back step by step through sharp electric jar removal in proper order quantitative injection a plurality of serial number experiment sample dilution mixing cylinder in the mixing cylinder is diluted to part experiment sample in proper order, part experiment sample dilution mixing cylinder includes: the test sample diluting and mixing cylinders except the first and the last ones in the plurality of test sample diluting and mixing cylinders which are numbered in sequence; injecting the diluent in the part of the experimental sample dilution mixing cylinder into the corresponding experimental sample storage cylinder through the conduction of the hose valve; injecting the test sample in the test sample storage cylinder into the corresponding aquatic organism experiment cylinder unit through the conduction of a hose valve; and the aquatic organism experiment cylinder unit discharges the waste liquid into a drainage collecting tank through a drainage pipe.
The test device provided by the embodiment of the invention comprises: the device comprises a cosolvent quantitative filling unit, a solvent storage tank, a solvent quantitative filling unit, an automatic gradient equivalent step-by-step liquid medicine preparation unit, an experimental sample mother liquid quantitative filling unit, a linear electric cylinder slide block arranged on a linear electric cylinder track, a plurality of sequentially numbered experimental sample diluting and mixing cylinders, a plurality of sequentially numbered experimental sample storage cylinders, a plurality of sequentially numbered aquatic organism experimental cylinder units and a drainage collecting tank arranged at the bottom of the sequentially numbered aquatic organism experimental cylinder units; wherein, the number of the experimental sample diluting and mixing cylinder, the experimental sample storage cylinder and the aquatic organism experimental cylinder unit 9 is consistent. The test device can realize automatic gradual dilution of an experimental sample, automatic filling equal-quantity periodic updating of an experimental sample solution, and testing of the reproductive toxicity of the running water type aquatic organisms.
Drawings
Fig. 1 is a schematic structural diagram of a testing apparatus according to an embodiment of the present invention;
a cosolvent quantitative filling unit-1, a solvent storage tank-2, a solvent quantitative filling unit-3, an automatic gradient equivalent step-by-step liquid medicine preparation unit-4, an experimental sample mother liquid quantitative filling unit-5, a linear electric cylinder slide block-6, an experimental sample dilution mixing cylinder-7, an experimental sample storage cylinder-8, an aquatic organism experimental cylinder unit-9, a liquid feeding collection port-10, a drainage collection tank-11, a linear electric cylinder track-12, a hose valve-13 and a magnetic stirrer-14.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be described clearly below with reference to the drawings and the embodiments in the embodiments of the present invention.
Fig. 1 is a schematic structural diagram of a testing apparatus according to an embodiment of the present invention, and as shown in fig. 1, the testing apparatus specifically includes:
a cosolvent quantitative filling unit 1, a solvent storage tank 2, a solvent quantitative filling unit 3, an automatic gradient equivalent step-by-step liquid medicine preparation unit 4, an experimental sample mother liquor quantitative filling unit 5, a linear electric cylinder slide block 6 arranged on a linear electric cylinder track 12, a plurality of experimental sample dilution mixing cylinders 7 numbered in sequence, a plurality of experimental sample storage cylinders 8 numbered in sequence, a plurality of liquid feeding collection ports 10 arranged on the plurality of experimental sample dilution mixing cylinders 7 and the last experimental sample storage cylinders, a plurality of aquatic organism experimental cylinder units 9 numbered in sequence, and a bottom drainage collection tank 11 arranged on the plurality of aquatic organism experimental cylinder units 9 numbered in sequence; wherein, the numbers of the experimental sample diluting and mixing cylinder 7, the experimental sample storage cylinder 8 and the aquatic organism experimental cylinder unit 9 are consistent.
In the present embodiment, the number of the experimental sample diluting and mixing tank 7, the experimental sample storage tank 8, and the aquatic organism experimental tank unit 9 is set to 8.
Optionally, the cosolvent quantitative filling unit 1 is communicated with a filling liquid collecting port 10 of a first experimental sample diluting and mixing cylinder 7 of the plurality of sequentially numbered experimental sample diluting and mixing cylinders 7 through a hose, wherein the experimental sample diluting and mixing cylinder 7 is provided with a filling liquid collecting port 10 corresponding to the experimental sample diluting and mixing cylinder.
Optionally, the automatic gradient equivalent stepwise liquid medicine preparation unit 4 and the quantitative filling unit 5 of the experimental sample mother liquor are communicated with the liquid filling collecting ports 10 of the last experimental sample diluting and mixing cylinders 7 of the plurality of sequentially numbered experimental sample diluting and mixing cylinders 7 through hoses.
Optionally, the solvent storage tank 2 is communicated with the solvent quantitative filling unit 3 and the automatic gradient equivalent step-by-step liquid medicine preparation unit 4 through hoses; the hose of the solvent quantitative filling unit 3 and the hose of the automatic gradient equivalent progressive liquid medicine preparation unit 4 are fixed with the linear electric cylinder slide block 6, so that liquid medicines in the solvent quantitative filling unit 3 and the automatic gradient equivalent progressive liquid medicine preparation unit 4 quantitatively flow into the experimental sample dilution mixing cylinder 7 through the sliding of the linear electric cylinder slide block 6.
Alternatively, a hose is provided at the bottom of a set portion of the plurality of sequentially-numbered test sample dilution mixing cylinders 7 and a hose valve 13 is provided on the hose, wherein the set portion includes a portion excluding the last ones of the plurality of sequentially-numbered test sample dilution mixing cylinders 7; the bottom of the experimental sample dilution mixing cylinder 7 is provided with a magnetic stirrer 14.
Optionally, the hose arranged in the experimental sample diluting and mixing cylinder 7 is communicated with the experimental sample storage cylinder 8 with the corresponding number, and the last experimental sample storage cylinder 8 of the multiple experimental sample storage cylinders 8 with the serial numbers is provided with a liquid feeding collection port 10 corresponding to the last experimental sample storage cylinder 8.
Alternatively, the bottom of the experimental sample storage cylinder 8 is provided with a hose and a hose valve 13 is provided on the hose, and the bottom of the experimental sample storage cylinder 8 is provided with a magnetic stirrer 14.
Optionally, the hoses arranged in the experimental sample storage cylinder 8 are communicated with the aquatic organism experimental cylinder units 9 with corresponding numbers.
Optionally, a drain pipe is arranged at the bottom of the aquatic organism experimental cylinder unit 9, and the drain pipe is communicated with the drain collecting tank 11.
The use method of the test device is as follows:
a cosolvent quantitative filling unit is used for injecting a cosolvent mother solution into a liquid filling collecting port of a first experimental sample diluting and mixing cylinder in a plurality of sequentially numbered experimental sample diluting and mixing cylinders through a hose; the experimental sample mother liquor quantitative filling unit is used for filling the experimental sample mother liquor into liquid adding collecting openings of last experimental sample dilution mixing cylinders in a plurality of sequentially numbered experimental sample dilution mixing cylinders through hoses; the solvent quantitative filling unit extracts a solvent from a solvent storage tank, movably fills the solvent into the last experimental sample diluting and mixing cylinder of the plurality of sequentially numbered experimental sample diluting and mixing cylinders through a linear electric cylinder, and fully stirs the solvent to form primary experimental sample mother liquor diluent; the automatic gradient equivalent step-by-step liquid medicine preparation unit extracts a solvent from a solvent storage tank and extracts a primary diluted mother liquid from the last experimental sample dilution mixing cylinder in the plurality of sequentially numbered experimental sample dilution mixing cylinders for mixing; dilute the mixed liquid step by step according to the set parameter and move through sharp electric jar and pour into a plurality of serial number experiment sample in proper order dilution of sample mix jar in the mixed jar is diluted to part experiment sample, and part experiment sample dilutes the mixed jar and includes: the test sample diluting and mixing cylinders except the first test sample diluting and mixing cylinder and the last test sample diluting and mixing cylinder are numbered in sequence; injecting the diluent in the part of the experimental sample dilution mixing cylinder into the corresponding experimental sample storage cylinder through the conduction of the hose valve; injecting the test sample in the test sample storage cylinder into the corresponding aquatic organism experiment cylinder unit through the conduction of the hose valve; and the aquatic organism experiment cylinder unit discharges the waste liquid into a drainage collecting tank through a drainage pipe.
The following is specifically described in the case where the number of the experimental sample dilution and mixing tank 7, the experimental sample storage tank 8, and the aquatic organism experimental tank unit 9 is set to 8:
it should be noted that: the bottom of the solvent quantitative filling unit is provided with a solvent adding pipe, and the automatic gradient equivalent step-by-step liquid medicine preparation unit is provided with a medicine adding pipe.
The medicine adding pipe and the solvent adding pipe are moved to a liquid adding collecting opening of an experimental sample diluting and mixing cylinder with the code of 7-2 through linear electric cylinder movement, an automatic gradient equivalent step-by-step liquid medicine preparing unit extracts an experimental sample mother liquid primary diluting solution from the experimental sample diluting and mixing cylinder with the code of 7-8 according to set parameters, then a part of the experimental sample mother liquid primary diluting solution is quantitatively added into the experimental sample diluting and mixing cylinder with the code of 7-2 according to the set parameters, and meanwhile, the solvent quantitatively adding unit quantitatively adds a solvent into the experimental sample diluting and mixing cylinder with the code of 7-2 according to the set parameters, so that the preparation of the experimental sample diluted solution with the first gradient concentration is completed. And moving the medicine adding pipe and the solvent adding pipe to a liquid adding collecting port of the experimental sample dilution mixing cylinder with the code number of 7-3 by linear electric cylinder movement, and quantitatively extracting the solvent from the solvent storage tank by the automatic gradient equivalent step-by-step liquid medicine preparation unit according to set parameters to complete preparation of the experimental sample degressive second gradient concentration preparation solution. After the second gradient preparation solution is prepared, the automatic gradient equivalent step-by-step liquid medicine preparation unit is used for filling the experimental sample diluting and mixing cylinder with the code number of 7-3 with the second gradient concentration preparation solution in a descending manner according to set parameters, and meanwhile, the solvent quantitative filling unit is used for quantitatively filling the solvent into the experimental sample diluting and mixing cylinder with the code number of 7-3 according to the set parameters, so that the preparation of the experimental sample gradient descending second gradient dilution solution is completed. And moving the medicine adding pipe and the solvent adding pipe to a liquid adding collecting port of the experimental sample diluting and mixing tank with the code number of 7-4 by linear electric cylinder movement, and quantitatively extracting the solvent from the solvent storage tank by the automatic gradient equivalent step-by-step liquid medicine preparation unit according to set parameters to complete preparation of the experimental sample degressive third gradient concentration preparation solution. And after the third gradient concentration preparation solution is prepared, filling the experiment sample dilution mixing cylinder with the code number of 7-4 with the experiment sample degressive third gradient concentration preparation solution by an automatic gradient equivalent step-by-step liquid medicine preparation unit according to set parameters, and simultaneously filling the solvent into the experiment sample dilution mixing cylinder with the code number of 7-4 by a solvent quantitative filling unit according to the set parameters to complete the preparation of the experiment sample gradient degressive third gradient dilution solution. And moving the medicine adding pipe and the solvent adding pipe to a liquid adding collecting port of the experimental sample dilution mixing cylinder with the code number of 7-5 by linear electric cylinder movement, and quantitatively extracting the solvent from the solvent storage tank by the automatic gradient equivalent step-by-step liquid medicine preparation unit according to set parameters to complete preparation of the experimental sample progressive-decrease fourth gradient concentration preparation solution. After the fourth gradient concentration preparation solution is prepared, the automatic gradient equivalent step-by-step liquid medicine preparation unit is used for filling the experiment sample dilution mixing cylinder with the code number of 7-5 with the experiment sample decrement fourth gradient concentration preparation solution according to the set parameters, and meanwhile, the solvent quantitative filling unit is used for quantitatively filling the solvent into the experiment sample dilution mixing cylinder with the code number of 7-5 according to the set parameters, so that the preparation of the experiment sample gradient decrement fourth gradient dilution solution is completed. And moving the medicine adding pipe and the solvent adding pipe to a liquid adding collecting port of the experimental sample diluting and mixing tank with the code number of 7-6 by linear electric cylinder movement, and quantitatively extracting the solvent from the solvent storage tank by the automatic gradient equivalent step-by-step liquid medicine preparation unit according to set parameters to complete preparation of the experimental sample degressive fifth gradient concentration preparation solution. After the fifth gradient concentration preparation solution is prepared, the automatic gradient equivalent step-by-step liquid medicine preparation unit is used for filling the experiment sample diluting and mixing cylinder with the code number of 7-6 with the experiment sample gradually-decreased fifth gradient concentration preparation solution according to the set parameters, and meanwhile, the solvent quantitative filling unit is used for quantitatively filling the solvent into the experiment sample diluting and mixing cylinder with the code number of 7-6 according to the set parameters, so that the preparation of the experiment sample gradient gradually-decreased fifth gradient dilution solution is completed. And moving the medicine adding pipe and the solvent adding pipe to a liquid adding collecting port of the experimental sample dilution mixing cylinder with the code number of 7-7 by linear electric cylinder movement, and quantitatively extracting the solvent from the solvent storage tank by the automatic gradient equivalent step-by-step liquid medicine preparation unit according to set parameters to complete preparation of the experimental sample progressive-decrease sixth gradient concentration preparation solution. After the sixth gradient experiment sample solution is prepared, the automatic gradient equivalent step-by-step liquid medicine preparation unit is used for filling the experiment sample dilution mixing cylinder with the code number of 7-7 with the experiment sample decrement sixth gradient concentration preparation solution according to the set parameters, and meanwhile, the solvent quantitative filling unit is used for quantitatively filling the solvent into the experiment sample dilution mixing cylinder with the code number of 7-7 according to the set parameters, so that the preparation of the experiment sample gradient decrement sixth gradient dilution solution is completed. The preparation of the primary diluted solution of the experimental sample mother liquor according to 6 secondary diluted solutions with descending gradient is completed through the processes.
And then moving the solvent adding pipe to a liquid adding collecting port of the experimental sample diluting and mixing cylinder with the code of 7-1 by linear electric cylinder movement, quantitatively adding the solvent to the experimental sample diluting and mixing cylinder with the code of 7-1 by a solvent quantitative adding unit according to set parameters, and quantitatively adding the cosolvent to the experimental sample diluting and mixing cylinder with the code of 7-1 by a cosolvent quantitative adding unit according to the set parameters to finish the preparation of the diluting solution of the cosolvent.
And (3) opening a hose valve at the outlet of the experimental sample diluting and mixing cylinder with the code numbers of 7-1 to 7-7 at regular time according to set parameters to respectively supplement the solution to the experimental sample storage cylinders with the code numbers of 8-1 to 8-7, and simultaneously moving the solvent adding pipe to the liquid adding collecting port of the experimental sample storage cylinder with the code number of 8-8 by the linear electric cylinder to quantitatively add the solvent to the experimental sample storage cylinder with the code number of 8-8 according to the set parameters.
And (3) regularly opening hose valves at the outlets of the experimental sample storage cylinders with the codes of 8-1 to 8-8 according to set parameters to respectively supplement the solution to the aquatic organism experimental cylinder units with the codes of 9-1 to 9-8. The solutions at different levels, the diluted solution of the cosolvent mother liquor and the solvent which are diluted step by step according to equal quantity of gradient are updated once.
By the method, the automatic dilution of the experimental sample, the automatic filling and equivalent periodic updating of the experimental sample solution and the test of the reproductive toxicity of the running water type aquatic organisms can be realized.
Thus, it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been illustrated and described in detail herein, many other variations or modifications consistent with the principles of the invention may be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the invention. Accordingly, the scope of the invention should be understood and interpreted to cover all such other variations or modifications.
Claims (7)
1. A test apparatus, comprising: the device comprises a cosolvent quantitative filling unit (1), a solvent storage tank (2), a solvent quantitative filling unit (3), an automatic gradient equivalent step-by-step liquid medicine preparation unit (4), an experimental sample mother liquid quantitative filling unit (5), a linear electric cylinder slider (6) arranged on a linear electric cylinder track (12), a plurality of sequentially numbered experimental sample diluting and mixing cylinders (7), a plurality of sequentially numbered experimental sample storage cylinders (8), a plurality of sequentially numbered aquatic organism experimental cylinder units (9), a plurality of liquid feeding collection ports (10) arranged on the plurality of experimental sample diluting and mixing cylinders (7) and the last experimental sample storage cylinders, and a drainage collection tank (11) arranged at the bottom of the aquatic organism experimental cylinder unit (9);
wherein the numbers of the experimental sample diluting and mixing cylinder (7), the experimental sample storage cylinder (8) and the aquatic organism experimental cylinder unit (9) are consistent;
the cosolvent quantitative filling unit (1) is communicated with a liquid adding collecting port (10) of a first experimental sample diluting and mixing cylinder (7) in the plurality of sequentially numbered experimental sample diluting and mixing cylinders (7) through a hose, wherein the experimental sample diluting and mixing cylinder (7) is provided with a liquid adding collecting port (10) corresponding to the experimental sample diluting and mixing cylinder;
the automatic gradient equivalent step-by-step liquid medicine preparation unit (4) and the experimental sample mother liquid quantitative filling unit (5) are communicated with the liquid adding collecting ports (10) of the last experimental sample diluting and mixing cylinders (7) in the plurality of sequentially numbered experimental sample diluting and mixing cylinders (7) through hoses;
the solvent storage tank (2) is communicated with the solvent quantitative filling unit (3) and the automatic gradient equivalent step-by-step liquid medicine preparation unit (4) through a hose; the hose of the solvent quantitative filling unit (3) and the hose of the automatic gradient equivalent progressive liquid medicine preparation unit (4) are fixed to the linear electric cylinder sliding block (6), so that the solvent of the solvent quantitative filling unit (3) and the liquid medicine in the automatic gradient equivalent progressive liquid medicine preparation unit (4) sequentially flow into the experimental sample dilution mixing cylinder (7) according to a preset sequence through the sliding of the linear electric cylinder sliding block (6).
2. The device according to claim 1, wherein a hose is provided at the bottom of the set portion of the plurality of sequentially numbered test sample dilution mixing cylinders (7) and a hose valve (13) is provided on the hose, wherein the set portion comprises a portion excluding the last of the plurality of sequentially numbered test sample dilution mixing cylinders (7);
and a magnetic stirrer (14) is arranged at the bottom of the experimental sample dilution mixing cylinder (7).
3. The device according to claim 2, characterized in that the hoses arranged in the test sample dilution and mixing cylinders (7) are in communication with correspondingly numbered test sample storage cylinders (8), and the last test sample storage cylinder (8) of the plurality of sequentially numbered test sample storage cylinders (8) is provided with a filling collection port (10) corresponding thereto.
4. The device according to claim 3, characterized in that the bottom of the laboratory sample storage cylinder (8) is provided with a hose and a hose valve (13) is provided on the hose, and the bottom of the laboratory sample storage cylinder (8) is provided with a magnetic stirrer (14).
5. The apparatus according to claim 4, wherein the hose provided to the test sample storage cylinder (8) is communicated with the corresponding number of aquatic organism test cylinder units (9).
6. The apparatus according to claim 4, wherein a drain pipe is provided at the bottom of the aquatic organism testing cylinder unit (9) and communicates with the drain collecting tank (11).
7. A method of use using a test device according to any one of claims 1 to 6, comprising:
injecting a cosolvent mother liquor into a liquid adding collecting port of a first experimental sample diluting and mixing cylinder in a plurality of sequentially numbered experimental sample diluting and mixing cylinders by a cosolvent quantitative filling unit through a hose;
step two, quantitatively injecting the experimental sample mother liquor into liquid adding collecting ports of last experimental sample dilution mixing cylinders in the plurality of experimental sample dilution mixing cylinders which are sequentially numbered by a solvent quantitative filling unit through hoses, and quantitatively injecting a solvent into the liquid adding collecting ports of the last experimental sample dilution mixing cylinders in the plurality of experimental sample dilution mixing cylinders which are sequentially numbered by the solvent quantitative filling unit through hoses to fully stir to form primary diluent of the mother liquor;
step three, extracting a primary diluent of the mother liquor by an automatic gradient equivalent step-by-step liquid medicine preparation unit according to set parameters, quantitatively injecting part of the primary diluent of the mother liquor into a second experimental sample dilution mixing cylinder, quantitatively injecting a solvent into the second experimental sample dilution mixing cylinder by a solvent quantitative injection unit, and fully stirring to form a first gradient concentration diluent;
step four, the automatic gradient equivalent step-by-step liquid medicine preparation unit quantitatively extracts the solvent to form a transition concentration solution from the first gradient concentration diluted solution to the second gradient concentration diluted solution, then quantitatively injects part of the transition concentration solution into a third experimental sample dilution mixing cylinder, and simultaneously the solvent quantitative injection unit quantitatively injects the solvent into the third experimental sample dilution mixing cylinder, and fully stirs to form the second gradient concentration diluted solution;
repeating the step four for multiple times, and forming a plurality of gradient concentration diluted solutions with sequentially decreasing concentrations in a plurality of experimental sample dilution mixing cylinders except the last experimental sample dilution mixing cylinder;
injecting the fully stirred diluent in the part of the experimental sample diluting and mixing cylinder into the corresponding experimental sample storage cylinder through the conduction of the hose valve;
injecting the test sample fully stirred in the test sample storage cylinder into the corresponding aquatic organism experiment cylinder unit through the conduction of a hose valve;
and the aquatic organism experiment cylinder unit discharges the waste liquid into a drainage collecting tank through a drainage pipe.
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| CN111505324A (en) * | 2020-05-13 | 2020-08-07 | 安图实验仪器(郑州)有限公司 | Automatic solution diluting method |
| CN114051957A (en) * | 2021-11-15 | 2022-02-18 | 江苏雅信昆成检测科技有限公司 | Large-scale daphnia of continuous-flow type test device |
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