CN107456438A - Cosmetic composition - Google Patents

Abstract

The present invention relates to cosmetic compositions. In particular, compositions and methods that can be used to improve the visual appearance of skin are disclosed. In some aspects, the compositions disclosed herein can include, for example, ingredients for exfoliating, reducing or eliminating irritation due to exfoliation, renewing the skin, increasing skin regeneration, increasing skin radiance, soothing the skin, or increasing the smoothness of the skin. combination. This combination of ingredients can be included in a wide range of product formulations (eg, serums, eye creams, toners, gels, masks, masks, etc.).

Description

cosmetic composition

technical field

The present invention generally relates to compositions and methods that can be used to improve the visual appearance of skin. In some aspects, the compositions disclosed herein can include, for example, for exfoliating, reducing or eliminating irritation due to exfoliation, renewing the skin, regenerating the skin, increasing skin radiance, soothing the skin, and/or increasing the smoothness of the skin. Combination of ingredients.

Background technique

Aging, chronic exposure to adverse environmental factors, nutritional disorders, fatigue, etc. can alter the visual appearance, physical properties or physiological functions of the skin in ways that are considered visually undesirable. The most noticeable and noticeable changes include the development of fine lines and wrinkles, loss of elasticity, increased sagging, loss of firmness, loss of color uniformity or tone, rough surface texture, and mottled pigmentation. Subtle but measurable changes that occur as skin ages or experiences long-term environmental damage include a general decrease in cell and tissue activity, a decrease in the rate of cell replication, decreased blood flow to the skin, decreased moisture content, accumulated structural and malfunctions, changes in the normal regulation of common biochemical processes, and a reduction in the skin's ability to remodel and repair itself. Many changes in the appearance and function of the skin result from changes in the outer epidermal layer of the skin, while other changes result from changes in the underlying dermis.

Previous attempts to improve the visual appearance of the skin using known skin active ingredients have been shown to suffer from various drawbacks such as skin irritation and long recovery periods.

Contents of the invention

The present inventors have determined that various compounds, compositions and extracts have therapeutic effects. In particular, the present inventors have identified a method for exfoliating, reducing or eliminating skin irritation caused by exfoliating the skin, renewing the skin, increasing skin regeneration, increasing involucrin production in the skin and/or increasing skin radiance and/or A set of preparations for smoothness. This results in a product with excellent exfoliating properties without some of the negative effects typically associated with exfoliating products and/or a product capable of reducing the undesired side effects of skin exfoliation. A group of formulations has been identified that work together to provide an activity that is not present in the individual components of the formulation.

In some aspects, disclosed are those capable of exfoliating, capable of reducing or eliminating irritation from exfoliation, capable of skin renewal, capable of increasing skin regeneration, capable of increasing involucrin production in the skin, and/or capable of increasing skin radiance and/or smoothness A topical skin composition comprising any one, any combination, or all of the following ingredients: water, Mucor miehei extract, Bacillus fermentation product, plankton extract, nopal flower extract, and/or Hydrolyzed Prickly Pear Flower Extract. In some examples, the Mucor miehei extract is an aqueous extract that contains aspartyl-dependent water-soluble enzymes or acid proteases, and is capable of exfoliating the skin, increasing skin regeneration, and/or increasing the expression of involucrin. produce. In some examples, the composition contains a Bacillus ferment, which is a fermentation product of Bacillus subtilis, and which is capable of exfoliating the skin, increasing skin regeneration, and/or increasing involucrin production. In some examples, the plankton extract is an aqueous extract containing exopolysaccharide synthesized by Vibrio alginolyticus and capable of conditioning the skin, reducing inflammation in the skin, increasing skin regeneration, and/or increasing involucrin production . In some examples, the composition contains nopal cactus flower extract, which is an aqueous extract and is capable of increasing skin regeneration and/or increasing involucrin production. In some examples, the composition comprises hydrolyzed Prickly Pear flower extract, wherein the Prickly Pear flower extract is an aqueous extract, and wherein the hydrolyzed Prickly pear flower extract is capable of exfoliating the skin. In some examples, the composition further comprises Silicone-11, Cyclopentasiloxane, Dimethicone, Glycerin, PEG-10 Dimethicone, Butylene Glycol, Bis-PEG -18 Methyl ether dimethylsilane, phenoxyethanol, ammonium acryloyldimethyltaurate/VP copolymer, propylene glycol, biosugar gum-1, acacia gum extract, hydroxyethyl acrylate/acryloyl di Sodium methyl taurate copolymer, squalene, decanediol, sodium citrate, hydroxyethyl cellulose, 1,2-hexanediol, citric acid, dipotassium glycyrrhizinate, tocopheryl acetate, yellow Raw gum, hydroxypropyl cyclodextrin, triethanolamine, polysorbate 60, disodium EDTA, sorbitan isostearate, and/or potassium sorbate. Concentrations of these ingredients may range from 0.00001% to 99% by weight or volume of the composition, or any integer or range available therein as described elsewhere in this specification, which is incorporated herein by reference. part. In a specific aspect, the composition comprises 40% to 60% by weight of water, 0.01% to 1% by weight of Mucor miehei extract, 0.001% to 1% by weight of Bacillus fermentation product, 0.001% to 1% by weight of 0.1 wt% plankton extract, 0.01 wt% to 1 wt% hydrolyzed prickly pear flower extract or nopal flower extract, 10 wt% to 30 wt% polysiloxane-11, 5 wt% to 20 wt% Cyclopentasiloxane, 1 wt% to 10 wt% Dimethicone, 1 wt% to 10 wt% Glycerin, 1 wt% to 10 wt% PEG-10 Dimethicone siloxane, 0.5% to 5% by weight of butanediol, 0.5% to 5% by weight of bis-PEG-18 methyl ether dimethyl silane, 0.1% to 3% by weight of phenoxyethanol, 0.1% to 3% by weight of ammonium acryloyl dimethyl taurate / VP copolymer, 0.1% to 3% by weight of propylene glycol, 0.1% to 3% by weight of bioglucose gum-1, 0.1% by weight to 1.5% by weight of acacia gum extract, 0.1 to 1.5% by weight of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, 0.1 to 1.5% by weight of squalene, 0.01% by weight to 1% by weight of decanediol, 0.01 to 1% by weight of sodium citrate, 0.01 to 1% by weight of hydroxyethylcellulose, 0.01 to 1% by weight of 1,2-hexanediol , 0.01% to 1% by weight of citric acid, 0.01% to 1% by weight of dipotassium glycyrrhizinate, 0.01% to 1% by weight of tocopheryl acetate, 0.01% to 1% by weight of xanthan gum , 0.01 to 1% by weight hydroxypropyl cyclodextrin, 0.01 to 1% by weight triethanolamine, 0.01 to 1% by weight polysorbate 60, 0.01 to 1% by weight EDTA Disodium, 0.001% to 0.1% by weight sorbitan isostearate, and/or 0.001% to 0.1% by weight potassium sorbate.

In particular aspects, the concentration of water may be at least 35% to 80% by weight water. In some examples, the composition also includes adenosine. In some examples, the composition comprises 0.001% to 1% by weight adenosine. In some examples, the composition may also include Oputia tuna fruit extract. In some examples, the composition comprises 0.00001% to 0.01% by weight prickly pear fruit extract. In some examples, the composition may also include alpha-hydroxy acids. In some examples, the alpha-hydroxy acid is glycolic acid. In some examples, the composition is an emulsion. In some examples, the composition is an oil-in-water or water-in-oil emulsion.

Also disclosed are methods of using any of the compositions disclosed herein. In some aspects, disclosed herein are methods of counteracting skin irritation caused by exfoliating ingredients by topically applying any of the compositions disclosed herein to skin in need thereof. In one example, skin irritation can be caused by alpha hydroxy acids. In some aspects, disclosed herein are methods of exfoliating skin by topically applying any of the compositions disclosed herein to skin in need thereof. In some aspects, disclosed herein are methods of increasing skin regeneration by topically applying any of the compositions disclosed herein to skin in need thereof. In some aspects, disclosed herein are methods of increasing involucrin production in skin by topically applying any of the compositions disclosed herein to skin in need thereof. It is also disclosed that any of the methods disclosed herein may further comprise applying the composition to the face.

The following Embodiments 1 to 101 of the present invention are also disclosed. Embodiment 1 is a method of increasing skin regeneration comprising topically applying a composition comprising Mucor miehei extract, Bacillus ferment product, plankton extract, and hydrolyzed pomegranate flower extract or nopal flower extract. Embodiment 2 is the method of embodiment 1, wherein the composition comprises: 40% to 60% by weight water, 0.01% to 1% by weight Mucor miehei extract, 0.001% to 1% by weight Bacillus fermentation product, 0.001% to 1% by weight plankton extract, and 0.01% to 1% by weight hydrolyzed prickly pear flower extract or nopal flower extract. Embodiment 3 is the method of embodiment 1, wherein the composition further comprises 25% to 80% by weight water. Embodiment 4 is the method of embodiment 1, wherein the Mucor miehei extract is an aqueous extract containing aspartyl-dependent water-soluble enzymes or acid proteases, and wherein the Mucor miehei extract exfoliates the skin, Increase skin regeneration, and/or increase involucrin production. Embodiment 5 is the method of embodiment 1, wherein the Bacillus fermentation product is a fermentation product of Bacillus subtilis, and wherein the Bacillus fermentation product exfoliates the skin, increases skin regeneration, and/or increases involucrin production. Embodiment 6 is the method of embodiment 1, wherein the plankton extract is an aqueous extract containing exopolysaccharide synthesized by Vibrio alginolyticus, and wherein the plankton extract conditions the skin, reduces inflammation in the skin, increases skin regeneration , and/or increase involucrin production. Embodiment 7 is the method of embodiment 1 comprising the nopal flower extract, wherein the nopal flower extract is an aqueous extract and wherein the nopal flower extract increases skin regeneration and/or increases involucrin production. Embodiment 8 is the method of embodiment 1 comprising hydrolyzed Prickly Pear flower extract, wherein the Prickly Pear flower extract is an aqueous extract and wherein the hydrolyzed Prickly Pear flower extract exfoliates the skin. Embodiment 9 is the method of embodiment 1, wherein the composition further comprises: adenosine. Embodiment 10 is the method of embodiment 9, wherein the composition comprises 0.001% to 1% by weight adenosine. Embodiment 11 is the method of embodiment 1, wherein the composition further comprises: prickly pear fruit extract. Embodiment 12 is the method of embodiment 1, wherein the composition comprises 0.00001% to 0.01% by weight of prickly pear fruit extract. Embodiment 13 is the method of embodiment 1, wherein the composition is formulated as a facial mask. Embodiment 14 is the method of embodiment 13, wherein the composition further comprises an alpha hydroxy acid. Embodiment 15 is the method of embodiment 14, wherein the composition comprises 2% to 20% by weight of an alpha hydroxy acid. Embodiment 16 is the method of embodiment 15, wherein the alpha hydroxy acid is glycolic acid. Embodiment 17 is the method of embodiment 1, wherein the composition is an oil-in-water or water-in-oil emulsion. Embodiment 18 is the method of embodiment 1, further comprising applying the composition to the face. Embodiment 19 is a method of increasing involucrin production in the skin comprising topically applying a composition comprising: Mucor miehei extract, Bacillus fermentation product, plankton extract, and hydrolyzed Prickly Pear flower extract or nopal cactus flower extract. Embodiment 20 is the method of embodiment 19, wherein the composition comprises: 40% to 60% by weight water, 0.01% to 1% by weight Mucor miehei extract, 0.001% to 1% by weight Bacillus fermentation product, 0.001% to 0.1% by weight of plankton extract, and 0.01% to 1% by weight of hydrolyzed prickly pear flower extract or nopal flower extract. Embodiment 21 is the method of embodiment 19, wherein the composition further comprises 25% to 80% by weight water. Embodiment 22 is the method of embodiment 19, wherein the Mucor miehei extract is an aqueous extract containing aspartyl-dependent water-soluble enzymes or acid proteases, and wherein the Mucor miehei extract exfoliates the skin, Increase skin regeneration, and/or increase involucrin production. Embodiment 23 is the method of embodiment 19, wherein the Bacillus fermentation product is a fermentation product of Bacillus subtilis, and wherein the Bacillus fermentation product exfoliates the skin, increases skin regeneration, and/or increases involucrin production. Embodiment 24 is the method of embodiment 19, wherein the plankton extract is an aqueous extract containing exopolysaccharide synthesized by Vibrio alginolyticus, and wherein the plankton extract conditions the skin, reduces inflammation in the skin, increases skin regeneration , and/or increase involucrin production. Embodiment 25 is the method of embodiment 19 comprising the nopal flower extract, wherein the nopal flower extract is an aqueous extract and wherein the nopal flower extract increases skin regeneration and/or increases involucrin production. Embodiment 26 is the method of embodiment 19 comprising hydrolyzed Prickly Pear flower extract, wherein the Prickly Pear flower extract is an aqueous extract, wherein the hydrolyzed Prickly Pear flower extract exfoliates the skin. Embodiment 27 is the method of embodiment 19, wherein the composition further comprises: adenosine. Embodiment 28 is the method of embodiment 27, wherein the composition comprises 0.001% to 1% by weight adenosine. Embodiment 29 is the method of embodiment 19, wherein the composition further comprises: prickly pear fruit extract. Embodiment 30 is the method of embodiment 29, wherein the composition comprises 0.00001% to 0.01% by weight of prickly pear fruit extract. Embodiment 31 is the method of embodiment 19, wherein the composition is formulated as a facial mask. Embodiment 32 is the method of embodiment 31, wherein the composition further comprises an alpha hydroxy acid. Embodiment 33 is the method of embodiment 32, wherein the composition comprises 2% to 20% by weight of an alpha hydroxy acid. Embodiment 34 is the method of embodiment 33, wherein the alpha hydroxy acid is glycolic acid. Embodiment 35 is the method of embodiment 19, wherein the composition is an oil-in-water or water-in-oil emulsion. Embodiment 36 is the method of embodiment 19, further comprising applying the composition to the face. Embodiment 37 is a method of counteracting skin irritation caused by an exfoliating ingredient comprising topically applying to skin in need thereof a composition comprising: Mucor miehei extract; Bacillus ferment; plankton extract; and hydrolyzed prickly pear flower extract or nopal cactus flower extract. Embodiment 38 is the method of embodiment 37, wherein the composition comprises: 40% to 60% by weight water; 0.01% to 1% by weight Mucor miehei extract; 0.001% to 1% by weight Bacillus Fermentation product; 0.001% to 0.1% by weight plankton extract; and 0.01% to 1% by weight hydrolyzed prickly pear flower extract or nopal flower extract. Embodiment 39 is the method of embodiment 37, wherein the composition further comprises 25% to 80% by weight water. Embodiment 40 is the method of embodiment 37, wherein the Mucor miehei extract is an aqueous extract containing aspartyl-dependent water-soluble enzymes or acid proteases, and wherein the Mucor miehei extract exfoliates the skin, Increase skin regeneration, and/or increase involucrin production. Embodiment 41 is the method of embodiment 37, wherein the Bacillus fermentation product is a fermentation product of Bacillus subtilis, and wherein the Bacillus fermentation product exfoliates the skin, increases skin regeneration, and/or increases involucrin production. Embodiment 42 is the method of embodiment 37, wherein the plankton extract is an aqueous extract comprising exopolysaccharide synthesized by Vibrio alginolyticus, and wherein the plankton extract conditions the skin, reduces inflammation in the skin, increases skin regeneration , and/or increase involucrin production. Embodiment 43 is the method of embodiment 37, comprising the nopal flower extract, wherein the nopal flower extract is an aqueous extract and wherein the nopal flower extract increases skin regeneration and/or increases involucrin production. Embodiment 44 is the method of embodiment 37, comprising hydrolyzing the Prickly Pear flower extract, wherein the Prickly Pear flower extract is an aqueous extract, wherein the hydrolyzed Prickly Pear flower extract exfoliates the skin. Embodiment 45 is the method of embodiment 37, wherein the composition further comprises: adenosine. Embodiment 46 is the method of embodiment 45, wherein the composition comprises 0.001% to 1% by weight adenosine. Embodiment 47 is the method of embodiment 37, wherein the composition further comprises: prickly pear fruit extract. Embodiment 48 is the method of embodiment 47, wherein the composition comprises 0.00001% to 0.01% by weight of prickly pear fruit extract. Embodiment 49 is the method of embodiment 37, wherein the composition is formulated as a facial mask. Embodiment 50 is the method of embodiment 49, wherein the composition further comprises an alpha hydroxy acid. Embodiment 51 is the method of embodiment 50, wherein the composition comprises 2% to 20% by weight of an alpha hydroxy acid. Embodiment 52 is the method of embodiment 51, wherein the alpha hydroxy acid is glycolic acid. Embodiment 53 is the method of embodiment 37, wherein the skin irritation is caused by an alpha hydroxy acid. Embodiment 54 is the method of embodiment 37, wherein the composition is an oil-in-water or water-in-oil emulsion. Embodiment 55 is the method of embodiment 37, further comprising applying the composition to the face. Embodiment 56 is a method of exfoliating the skin comprising topically applying a composition comprising: Mucor miehei extract, Bacillus ferment product, plankton extract, and hydrolyzed prickly pear flower extract or nopal Cactus flower extract. Embodiment 57 is the method of embodiment 56, wherein the composition comprises: 40% to 60% by weight water, 0.01% to 1% by weight Mucor miehei extract, 0.001% to 1% by weight Bacillus Fermentation product, 0.001% to 0.1% by weight plankton extract, and 0.01% to 1% by weight hydrolyzed prickly pear flower extract or nopal flower extract. Embodiment 58 is the method of embodiment 56, wherein the composition further comprises 25% to 80% by weight water. Embodiment 59 is the method of embodiment 56, wherein the Mucor miehei extract is an aqueous extract containing aspartyl-dependent water-soluble enzymes or acid proteases, and wherein the Mucor miehei extract exfoliates the skin, Increase skin regeneration, and/or increase involucrin production. Embodiment 60 is the method of embodiment 56, wherein the Bacillus fermentation product is a fermentation product of Bacillus subtilis, and wherein the Bacillus fermentation product exfoliates the skin, increases skin regeneration, and/or increases involucrin production. Embodiment 61 is the method of embodiment 56, wherein the plankton extract is an aqueous extract comprising exopolysaccharide synthesized by Vibrio alginolyticus, and wherein the plankton extract conditions the skin, reduces inflammation in the skin, increases skin regeneration , and/or increase involucrin production. Embodiment 62 is the method of embodiment 56, comprising the nopal flower extract, wherein the nopal flower extract is an aqueous extract and wherein the nopal flower extract increases skin regeneration and/or increases involucrin production. Embodiment 63 is the method of embodiment 56, comprising hydrolyzing the Prickly Pear flower extract, wherein the Prickly Pear flower extract is an aqueous extract, wherein the hydrolyzed Prickly Pear flower extract exfoliates the skin. Embodiment 64 is the method of embodiment 56, wherein the composition further comprises: adenosine. Embodiment 65 is the method of embodiment 64, wherein the composition comprises 0.001% to 1% by weight adenosine. Embodiment 66 is the method of embodiment 56, wherein the composition further comprises: prickly pear fruit extract. Embodiment 67 is the method of embodiment 66, wherein the composition comprises 0.00001% to 0.01% by weight of prickly pear fruit extract. Embodiment 68 is the method of embodiment 56, wherein the composition is formulated as a facial mask. Embodiment 69 is the method of embodiment 68, wherein the composition further comprises an alpha hydroxy acid. Embodiment 70 is the method of embodiment 69, wherein the composition comprises 2% to 20% by weight of an alpha hydroxy acid. Embodiment 71 is the method of embodiment 70, wherein the alpha hydroxy acid is glycolic acid. Embodiment 72 is the method of embodiment 56, wherein the composition is an oil-in-water or water-in-oil emulsion. Embodiment 73 is the method of embodiment 56, further comprising applying the composition to the face. Embodiment 74 is a topical skin composition comprising: Mucor miehei extract, Bacillus ferment product, plankton extract, and hydrolyzed prickly pear flower extract or nopal flower extract. Embodiment 75 is the topical skin composition of embodiment 74, wherein the composition comprises: 40% to 60% by weight water, 0.01% to 1% by weight Mucor miehei extract, 0.001% to 1% by weight % of Bacillus fermentation product, 0.001% to 0.1% by weight of plankton extract, and 0.01% to 1% by weight of hydrolyzed prickly pear flower extract or nopal flower extract. Embodiment 76 is the topical skin composition of embodiment 74, wherein the Mucor miehei extract is an aqueous extract containing aspartyl-dependent water-soluble enzymes or acid proteases, and wherein the Mucor miehei extract makes Skin exfoliation, increased skin regeneration, and/or increased involucrin production. Embodiment 77 is the topical skin composition of embodiment 74, wherein the Bacillus fermentation product is a fermentation product of Bacillus subtilis, and wherein the Bacillus fermentation product exfoliates the skin, increases skin regeneration, and/or increases involucrin production . Embodiment 78 is the topical skin composition of embodiment 74, wherein the plankton extract is an aqueous extract comprising exopolysaccharide synthesized by Vibrio alginolyticus, and wherein the plankton extract conditions the skin, reduces inflammation in the skin , increase skin regeneration, and/or increase involucrin production. Embodiment 79 is the topical skin composition of embodiment 74, comprising nopal flower extract, wherein the nopal flower extract is an aqueous extract and wherein the nopal flower extract increases skin regeneration and/or increases involucrin production . Embodiment 80 is the topical skin composition of embodiment 74, comprising hydrolyzed Prickly Pear flower extract, wherein the Prickly Pear flower extract is an aqueous extract, wherein the hydrolyzed Prickly pear flower extract exfoliates the skin. Embodiment 81 is the topical skin composition of embodiment 74, further comprising: Silicone-11, Cyclopentasiloxane, Dimethicone, Glycerin, PEG-10 Dimethicone alkanes, butanediol and bis-PEG-18 methyl ether dimethylsilane. Embodiment 82 is the topical skin composition of embodiment 81 comprising: 10% to 30% by weight Polysiloxane-11, 5% to 20% by weight Cyclopentasiloxane, 1% to 10 wt% polydimethylsiloxane, 1 wt% to 10 wt% glycerin, 1 wt% to 10 wt% PEG-10 dimethicone, 0.5 wt% to 5 wt% butyl Diol and 0.5% to 5% by weight of bis-PEG-18 methyl ether dimethylsilane. Embodiment 83 is the topical skin composition of embodiment 81, further comprising: phenoxyethanol, ammonium acryloyldimethyltaurate/VP copolymer, propylene glycol, biosaccharide gum-1, acacia gum extract, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer, Squalene, Decanediol, Sodium Citrate, Hydroxyethylcellulose, 1,2-Hexanediol, Citric Acid, Dipotassium Glycyrrhizinate , Tocopheryl Acetate and Xanthan Gum. Embodiment 84 is the topical skin composition of embodiment 83, comprising: 0.1% to 3% by weight phenoxyethanol, 0.1% to 3% by weight ammonium acryloyldimethyltaurate/VP copolymer , 0.1% to 3% by weight of propylene glycol, 0.1% to 3% by weight of bio-sugar gum-1, 0.1% to 1.5% by weight of acacia gum extract, 0.1% to 1.5% by weight of hydroxyethyl acrylate Ester/sodium acryloyldimethyl taurate copolymer, 0.1% to 1.5% by weight squalene, 0.01% to 1% by weight decanediol, 0.01% to 1% by weight sodium citrate, 0.01 to 1% by weight hydroxyethylcellulose, 0.01 to 1% by weight 1,2-hexanediol, 0.01 to 1% by weight citric acid, 0.01 to 1% by weight licorice Dipotassium dipotassium acid, 0.01% to 1% by weight of tocopheryl acetate and 0.01% to 1% by weight of xanthan gum. Embodiment 85 is the topical skin composition of embodiment 83, further comprising: hydroxypropyl cyclodextrin, triethanolamine, polysorbate 60, disodium EDTA, sorbitan isostearate, and sorbic acid potassium. Embodiment 86 is the topical skin composition of claim 85, comprising: 0.01% to 1% by weight hydroxypropyl cyclodextrin, 0.01% to 1% by weight triethanolamine, 0.01% to 1% by weight % polysorbate 60, 0.01% to 1% by weight disodium EDTA, 0.001% to 0.1% by weight sorbitan isostearate and 0.001% to 0.1% by weight potassium sorbate. Embodiment 87 is the topical skin composition of embodiment 74, comprising 25% to 80% by weight water. Embodiment 88 is the topical skin composition of embodiment 74, further comprising: adenosine. Embodiment 89 is the topical skin composition of embodiment 88, wherein the composition comprises 0.001% to 1% by weight of adenosine. Embodiment 90 is the topical skin composition of embodiment 74, wherein the composition further comprises: prickly pear fruit extract. Embodiment 91 is the topical skin composition of embodiment 90, wherein the composition comprises 0.00001% to 0.01% by weight of prickly pear fruit extract. Embodiment 92 is the topical skin composition of embodiment 74, wherein the composition is capable of counteracting skin irritation caused by exfoliating ingredients. Embodiment 93 is the topical skin composition of embodiment 92, wherein the composition is capable of counteracting skin irritation caused by alpha hydroxy acids. Embodiment 94 is the topical skin composition of embodiment 74, wherein the composition is capable of exfoliating the skin. Embodiment 95 is the topical skin composition of embodiment 74, wherein the composition is capable of increasing skin regeneration. Embodiment 96 is the topical skin composition of embodiment 74, wherein the composition is capable of increasing involucrin production in the skin. Embodiment 97 is the topical skin composition of embodiment 74, wherein the composition is formulated as a mask. Embodiment 98 is the topical skin composition of embodiment 97, further comprising an alpha hydroxy acid. Embodiment 99 is the topical skin composition of embodiment 98, comprising 2% to 20% by weight of an alpha hydroxy acid. Embodiment 100 is the topical skin composition of embodiment 99, wherein the alpha hydroxy acid is glycolic acid. Embodiment 101 is the topical skin composition of embodiment 74, wherein the composition is an oil-in-water or water-in-oil emulsion.

Either of the compositions may remain on the skin for 30 seconds, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, 50 or 60 minutes after topical application or longer. The skin in need of the composition may be skin with fine lines or wrinkles or uneven skin. Uneven skin may be skin with hyperpigmented skin, melasma, freckles, dark spots, age spots, brown spots, and the like. After the composition has been applied, the skin may be protected from irritation, redness, dryness, flaking or chapping. Protecting includes reducing the likelihood of the skin developing said symptoms after use of the skin exfoliating composition.

In particular aspects, the compositions disclosed herein are formulated as topical skin compositions. Compositions may have a dermatologically acceptable carrier or vehicle for the compounds, compositions and extracts. The composition may further comprise humectants or humectants, surfactants, silicone-containing compounds, UV agents, oils, non-naturally occurring compounds, and/or other ingredients identified in this specification or known in the art. The compositions may contain additional non-naturally occurring ingredients. The composition can be honeydew, cream, gel, essence, emulsion (such as oil in water, water in oil, silicone in water, water in silicone, water in oil in water, oil in water, water in oil oils, silicone oil-in-water, etc.), solutions (e.g., aqueous or water-alcoholic solutions), anhydrous vehicles (e.g., lipstick or powder), ointments, emulsions, pastes, aerosols, solid forms, ophthalmic gel etc. The composition may be in powder form (eg, dried, lyophilized granules, etc.). The composition may be formulated for topical skin application at least 1, 2, 3, 4, 5, 6, 7 or more times per day during use. In other aspects disclosed herein, the composition may be storage stable or color stable, or both. It is also contemplated that the viscosity of the composition can be selected to achieve the desired result, for example, depending on the type of composition desired, the viscosity of the composition can be from 1 cps to well over 1 million cps, or any range or integer obtainable therein (e.g. , 2cps, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80 measured on a Brookfield viscometer at 25°C with a TC axis at 2.5rpm . .

In specific examples, the extract in the composition may be from a whole organism or plant, and may be an aqueous extract. However, it is contemplated that parts of organisms or plants (e.g., roots, bark, sap, stems, leaves, flowers, seeds, leaves, stems, roots, flowers, seeds, sap, bark) may be used in addition to whole organisms or plants etc.), such that a part of the organism or plant is used to the exclusion of other parts of the organism or plant to prepare the extract. As mentioned above, the extract may be an aqueous extract, but may also be a non-aqueous extract. The extract can be extracted with alcohol (such as methanol, ethanol, propanol, butanol, etc.), glycol (such as butylene glycol, propylene glycol, etc.), oil, water, and the like. An extract may comprise parts, chemicals, etc. that do not occur together naturally, or do not occur together naturally in proportions or concentrations that occur in nature. The extracts can be included in compositions such as topical skin compositions, edible compositions, injectable compositions, oral compositions, pharmaceutical compositions, hair care compositions, and the like. The composition may comprise from 0.0001% to 20% by weight of said organisms or plants, organism parts or plant parts, and/or extracts thereof (or 0.001, 0.01, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, 60, 70, 80, 90, 99% or more, or any integer or range therein).

Compositions disclosed herein may also be modified to have a desired oxygen radical absorbance capacity (ORAC) value. In certain non-limiting aspects, the compositions disclosed herein or the plants, plant parts or extracts thereof referred to throughout this specification can be modified to have at least about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 70, 80, 90, 95, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000, 15000, 20000, 30000, 50000, 100000 or more, or any range of ORAC values obtainable therein.

The composition can have a pH of from about 6 to about 9 in non-limiting aspects. In other aspects, the pH can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14. The composition may contain triglycerides. Non-limiting examples include small, medium and large chain triglycerides. In some aspects, the triglyceride is a medium chain triglyceride (eg, caprylic triglyceride). The compositions may also contain preservatives. Non-limiting examples of preservatives include methylparaben, propylparaben, or a mixture of methylparaben and propylparaben.

The compositions disclosed herein may also comprise any one, any combination or all of the following additional ingredients: water, chelating agents, humectants, preservatives, thickeners, silicone-containing compounds, essential oils, structuring agents, vitamins , pharmaceutical ingredients, non-naturally occurring compounds, or antioxidants, or any combination of such ingredients or mixtures of such ingredients. In certain aspects, the composition may comprise at least two, three, four, five, six, seven, eight, nine, ten or all of the additional ingredients indicated in the preceding sentence. Non-limiting examples of such additional ingredients are noted throughout this specification and incorporated into this section by reference. Amounts of such ingredients may be from 0.0001% to 99.9% by weight or volume of the composition, or within any integer or range as disclosed elsewhere in this specification, which is incorporated by reference into this paragraph.

Kits comprising the compositions disclosed herein are also contemplated. In certain embodiments, the composition is contained in a container. The container may be a bottle, dispenser or pack. The container can dispense a predetermined amount of the composition. In certain aspects, compositions are dispensed as a spray, dollop or fluid. The container may contain markings on its surface. The mark can be a word, an abbreviation, a picture or a symbol.

It is also contemplated that the compositions disclosed throughout the specification may be used as leave-on or rinse-off compositions. For example, a leave-on composition can be applied topically to the skin and left on the skin for a period of time (e.g., at least 5, 6, 7, 8, 9, 10, 20, or 30 minutes, or at least 1, 2 , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or 24 hours, or overnight or composition throughout the day). Alternatively, a rinse-off composition may be a product that is intended to be applied to the skin and then removed or rinsed off (such as with water) from the skin over a period of time, such as less than 5, 4, 3, 2 or 1 minute. Examples of rinse-off compositions may be skin cleansers, shampoos, conditioners or soaps. Examples of leave-on compositions may be skin moisturizers, sunscreens, masks, night or day creams.

It is contemplated that any embodiment discussed in this specification can be practiced with respect to any method or composition of the invention, and vice versa. Additionally, the compositions disclosed herein can be used to practice the methods of the invention.

In one embodiment, the compositions disclosed herein may be pharmaceutically or cosmetically acceptable, or may have pleasant tactile properties. "Pharmaceutically acceptable", "cosmetically acceptable" and/or "pleasant tactile properties" describe compositions that have specific tactile properties that are pleasant to the skin (e.g., compositions that are not too watery or oily, have silky textured compositions, non-sticky or sticky compositions, etc.). Pharmaceutically or cosmetically acceptable may also relate to the creaminess or lubricity properties of the composition, or the moisture retention properties of the composition.

"Topical application" means applying or spreading the composition to the surface of the lips or keratinous tissue. "Topical skin compositions" include compositions suitable for topical application to the lips or keratinous tissue. Such compositions are generally dermatologically acceptable because they do not exhibit undue toxicity, incompatibility, instability, allergic response, etc. when applied to the lips or skin. The topical skin care compositions disclosed herein can have a selected viscosity to avoid significant dripping or pooling after application to the skin.

"Cornous tissue" includes the stratum corneum-containing layer configured as the outermost protective covering of mammals, and includes, but is not limited to, lips, skin, hair, and nails.

The term "about" or "approximately" is defined as being close to, and in one non-limiting embodiment the term is defined as within 10%, preferably within 5%, more preferably within 1% as understood by those of ordinary skill in the art %, most preferably within 0.5%.

The term "substantially" and variations thereof are defined as a majority but not necessarily all of what is specified as understood by those of ordinary skill in the art, and in one non-limiting embodiment essentially means within 10% of, Within 5%, within 1%, or within 0.5%.

The term "inhibit" or "reduce" or any variation of these terms includes any measurable reduction or complete inhibition of achieving the desired result. The terms "promote" or "increase" or any variation of these terms include proteins or molecules that achieve the desired result (eg, matrix proteins such as fibronectin, laminin, collagen, or elastin, or molecules such as hyaluronic acid) any measurable increase or generation of .

As the term is used in this specification and/or claims, the term "effective" means sufficient to achieve a desired, intended or desired result.

When used in conjunction with the term "comprises" in the claims and/or specification, an element may mean "a" without a quantifier preceding it, but it is also consistent with "one or more", "at least one" and "one or more". In one" means.

As used in this specification and claims, the words "comprises", "having", "comprising" or "containing" are inclusive or open-ended and do not exclude additional, non-recited elements or method steps.

Compositions and methods of use thereof may "comprise," "consist essentially of," or "consist of" any of the ingredients or steps disclosed throughout this specification. With respect to the transitional phrase "consisting essentially of", in one non-limiting aspect, the basic and novel features of the compositions and methods disclosed in this specification include that the compositions reduce or prevent symptoms associated with sensitive skin (such as erythema ) ability to appear on the user's skin.

Other objects, features and advantages of the present invention will become apparent from the following detailed description. It should be understood, however, that the detailed description and examples, while indicating specific embodiments of the invention, are given by way of illustration only. Additionally, it is intended that changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.

detailed description

In specific embodiments, any one or any combination of the following may be used: plankton extract, Mucor miehei extract, Bacillus ferment product, and nopal flower extract or hydrolyzed prickly pear flower extract . This combination of ingredients can be included in a wide range of product formulations (eg, serums, eye creams, lotions, gels, masks, peels, etc.).

As indicated above, several unique aspects of the invention disclosed herein are to exfoliate the skin, eliminate skin irritation due to skin exfoliation after or during exfoliation, renew the skin, increase skin regeneration, increase the epithelial protein production, and/or increase skin radiance and/or smoothness. This enables skin reduction by removing unwanted spots such as melasma, skin hyperpigmentation, age spots, freckles, dark spots, etc. to reduce the appearance of fine lines and wrinkles, or even skin tone benefits while reducing some unwanted side effects.

The following subsections describe non-limiting aspects of the invention in further detail.

A. Exfoliating and Repairing Compositions

The specific exfoliating compositions disclosed herein are designed to reduce or eliminate skin irritation due to exfoliation of the skin, exfoliate the skin while simultaneously reducing or eliminating skin irritation due to exfoliation of the skin, renew the skin, Increase skin regeneration, increase involucrin production in the skin, and/or increase skin radiance and/or smoothness. The compositions rely on the unique combination of Mucor miehei extract, Bacillus ferment product, plankton extract, hydrolyzed prickly pear flower extract, and/or nopal flower extract. An example of such a composition is provided in Example 1, Table 1 and Table 2. While Bacillus ferment products can be used as active skin exfoliating ingredients, glycolic acid or other acids and other exfoliating ingredients (eg, lactic acid, citric acid, enzymatic exfoliating ingredients, etc.) can also be used.

Plankton extracts are extracts obtained from marine biomass. It is known as a skin conditioning agent. It also provides fatty acids, antioxidants and zinc to the skin, as well as reduces skin inflammation and protects the skin from sun damage. Plankton extracts are commercially available from Barnet Products Corp. (USA) under the tradename Benoitine. In some embodiments, the plankton extract is an exopolysaccharide synthesized by a microorganism known as Vibrio alginolyticus and belonging to the Thalassoplankton family. In some embodiments, the planktonic extract is a sugar isomer, an exopolysaccharide synthesized by Vibrio alginolyticus. In some embodiments, the extract is an aqueous extract. In some embodiments, this ingredient is commercially available, eg, from Barnet, which offers sugar isomers under the trade name Benoiderm.

Mucor miehei extract is commercially available from Active Organics (USA) under the tradename ACTIZYME ^™ E3M-M. In some embodiments, the Mucor miehei extract is a mushroom-derived, aspartate-dependent, water-soluble enzyme, or acid protease. In some embodiments, the extract is an aqueous extract. In some embodiments, the Mucor miehei extract softens the skin.

Bacillus fermentation products are commercially available from Sederma (France) under the trade name KERATOLINE ^™ . In some embodiments, the Bacillus fermentation product is a fermentation product of Bacillus Subtilis. In some embodiments, the Bacillus ferment product can exfoliate the skin.

Hydrolyzed prickly pear flower extract is commercially available from SiLab (France) under the trade name EXFOLACTIVE ^™ EL PX. In some embodiments, the extract is an aqueous extract. In some embodiments, the hydrolyzed prickly pear flower extract can exfoliate the skin.

Nopal flower extract is an extract of the flower of the nopal cactus. This extract is commercially available from SiLab (France) under the trade name EXFOLACTIVE ^™ . In some embodiments, the extract can condition the skin.

Adenosine is a heterocyclic organic compound generally having the following structure:

Adenosine is commercially available from various sources (see International Dictionary and Handbook of Cosmetic Ingredient, 12th Edition, Vol. 1, p. 60 (2008), which is incorporated herein by reference).

The exfoliating and repairing compositions described above can be applied to the skin and left on the skin for a period of time (eg, at least 1, 2, 3, 4, 5, 10, 20, 30, or 60 minutes or more). Thereafter, the composition can be washed or peeled from the skin if desired.

C. Amount of ingredients

It is contemplated that the compositions disclosed herein may contain any amount of the ingredients discussed in this specification. The composition may also comprise any number of combinations of additional ingredients described throughout this specification (eg, pigments or additional cosmetic or pharmaceutical ingredients). The concentration of any ingredient in the composition may vary. For example, in a non-limiting embodiment, the composition in its final form may comprise, consist essentially of, or consist of, for example, at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005% , 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012%, 0.0013%, 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.00201%, 2%, 0.002 %, 0.0023%, 0.0024%, 0.0025%, 0.0026%, 0.0027%, 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0000 0.0039%, 0.0040%, 0.0041%, 0.0042%, 0.0043%, 0.0044%, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0004 , 0.0056%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.00701%, 0.007% %, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077%, 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.000000 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%, 0.0095%, 0.0096%, 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.030302% , 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650%, 0.06 75%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.115050%, 0.09% , 0.2000%, 0.2250%, 0.2500%, 0.2750%, 0.3000%, 0.3250%, 0.3500%, 0.3750%, 0.4000%, 0.4250%, 0.4500%, 0.4750%, 0.5000%, 0.5250%, 0.0550%, 0.060% %, 0.6250%, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0% 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7% , 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2%, 4.3%, 4.4 %, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7% , 7.8%, 7.9%, 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4 %, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70% , 75%, 80%, 85%, 90%, 95%, or 99%, or any range obtainable therein, referred to throughout the specification and claims at least one of the ingredients. In non-limiting aspects, the percentages can be calculated by weight or volume of the entire composition. Those of ordinary skill in the art will understand that the concentrations in a given composition may vary according to additions, substitutions and/or subtractions of ingredients.

D. Carrier

The compositions disclosed herein can be incorporated into all types of carriers. Non-limiting examples include emulsions (e.g., water-in-oil, water-in-oil-in-water, oil-in-water, silicone-in-water, water-in-silicone, oil-in-water-in-oil, oil-in-water-in-silicone emulsions), milk Creams, lotions, solutions (aqueous or hydro-alcoholic solutions), anhydrous vehicles (such as lipsticks and powders), gels and ointments. Variations and other suitable vehicles will be apparent to those of ordinary skill in the art and are suitable for use in the present invention. In certain aspects, it is important that the concentrations and combinations of compounds, ingredients and reagents be chosen in such a way that the combinations are chemically compatible and do not form complexes which precipitate from the final product.

E. Additional Ingredients

In addition to the combination of ingredients disclosed by the present inventors, the composition may also contain additional ingredients such as cosmetic ingredients and pharmaceutically active ingredients. Non-limiting examples of these additional ingredients are described in the following subsections.

1. Cosmetic ingredients

The CTFA International Cosmetic Ingredient Dictionary and Handbook (2004 and 2008) describe a variety of non-limiting cosmetic ingredients that can be used in the context of the present invention. Examples of these types of ingredients include: Fragrances (artificial and natural), dyes and coloring ingredients (e.g. Blue No. 1, Blue No. 1 Lake, Red No. 40, Titanium Dioxide, D&C Blue No. 4, D&C Green 5 D&C Orange No. 4, D&C Red No. 17, D&C Red No. 33, D&C Purple No. 2, D&C Yellow No. 10 and D&C Yellow No. 11), adsorbents, lubricants, solvents, humectants (including e.g. emollients, Humectants, film formers, occlusive agents, and agents that affect the natural moisturizing mechanism of the skin), water repellents, UV absorbers (physical and chemical absorbers such as p-aminobenzoic acid (“PABA”) and corresponding PABA derivatives , titanium dioxide, zinc oxide, etc.), essential oils, vitamins (such as A, B, C, D, E, and K), trace metals (such as zinc, calcium, and selenium), anti-irritants (such as steroids and NSAIDs ), plant extracts (such as aloe, citrus, cucumber extracts, ginkgo, ginseng, and rosemary), antimicrobials, antioxidants (such as BHT and tocopherols), chelating agents (such as disodium edetate and ethyl Tetrasodium diamine tetraacetate), preservatives (such as methylparaben and propylparaben), pH adjusters (such as sodium hydroxide and citric acid), absorbents (such as aluminum starch octenyl succinate , kaolin, corn starch, oat starch, cyclodextrins, talc, and zeolites), skin bleaching and lightening agents (such as hydroquinone and niacinamide lactate), humectants (such as sorbitol, urea, methylglucitol ether-20 and mannitol), exfoliating ingredients, water repellents (such as sodium magnesium hydroxide/aluminum stearate), skin conditioning agents (such as aloe extract, allantoin, bisabolol, ceramides, Methicone, Hyaluronic Acid, Biosugar Gum-1, Ethylhexylglycerin, Pentylene Glycol, Hydrogenated Polydecene, Octyldodecyl Oleate, and Dipotassium Glycyrrhizinate). Non-limiting examples of some of these ingredients are provided in the following subsections.

a. UV absorber

UV absorbers that can be used in combination with the compositions disclosed herein include chemical and physical sunscreens. Non-limiting examples of chemical sunscreens that may be used include p-aminobenzoic acid (PABA), PABA esters (PABA glycerides, pentyldimethanol PABA esters, and octyldimethanol PABA esters), PABA butyl esters, PABA ethyl esters, Ethyl dihydroxypropanol PABA ester, benzophenones (oxybenzone, sulfoisobenzone, benzophenone and benzophenone-1 to 12), cinnamate (octyl methoxycinnamate , isopentyl p-methoxycinnamate, octyl methoxycinnamate, cinoxate, methyl diisopropyl cinnamate, DEA salt of methoxy cinnamate, ethyl diisopropyl cinnamate , Glyceryl Caprylate Dimethoxycinnamate and Ethyl Methoxycinnamate), Cinnamate, Salicylate (Methyl Salicylate, Benzyl Salicylate, Ethylene Glycol Salicylate ester, isopropyl benzyl alcohol salicylate, etc.), anthranilate/ester, ethyl urocanate, promembrane ester, octyl salicylate, dibenzoylmethane derivatives (such as arginine Vobenzone), octocrylene, octyl triazone, galloyl gallate trioleate, glyceryl aminobenzoate, 2-hydroxy-1,4-naphthoquinone and dihydroxyacetone, ethylhexyl tri Azinonone, Dioctylbutyramide Triazone, Benzylidene Malonate Dimethicone, Terexylylene Dicamphorone Sulfonic Acid, Phenyl Dibenzimidazole Tetrasulfonic Acid Disodium Ester, Diethylaminohydroxybenzoyl Hexyl Benzoate, Bisdiethylaminohydroxybenzoyl Benzoate, Bisbenzoxazolylphenylethylhexyliminotriazine, Cresyltrizole Triazine Siloxane, Methylenebisbenzotriazolyltetramethylbutylphenol and Bisethylhexyloxyphenol Methoxyphenyl Triazine, 4-Methylbenzylidene Camphor and 4-Methoxycinnamon isoamyl acid. Non-limiting examples of physical sunscreens include kaolin, talc, petrolatum, and metal oxides such as titanium dioxide and zinc oxide.

b. Moisturizer

Non-limiting examples of humectants that can be used with the compositions disclosed herein include amino acids, chondroitin sulfate, diglycerin, erythritol, fructose, glucose, glycerin, glycerol polymers, ethylene glycol, 1,2, 6-hexanetriol, honey, hyaluronic acid, hydrogenated honey, hydrogenated starch hydrolyzate, inositol, lactitol, maltitol, maltose, mannitol, natural moisturizing factor, PEG-15 butanediol, polyglycerol sorbitol, Salts of pyrrolidone carboxylic acid, potassium PCA, propylene glycol, sodium glucuronate, sodium PCA, sorbitol, sucrose, trehalose, urea, and xylitol.

Other examples include acetylated lanolin, acetylated lanolin alcohol, alanine, algae extract, aloe vera, aloe vera extract, aloe vera gel, marshmallow extract, apricot (prunus armeniaca) kernel oil , arginine, arginine aspartate, arnica extract, aspartic acid, avocado (perseagratissima) oil, barrier sphingolipids, butanol, beeswax, behenyl alcohol, beta-glutene Sterols, birch (betula alba) bark extract, borage (borago officinalis) extract, ruscus aculeatus extract, butylene glycol, calendula flower extract, calendula oil, candelilla ( euphorbia cerifera) wax, canola oil, caprylic/capric triglycerides, cardamom (elettaria cardamomum) oil, carnauba (copernicia erifera) wax, carrot (daucus carota sativa) oil, castor plant (ricinus communis) oil, ceramides , ozokerite, cetearyl alcohol polyoxyethylene (5) ether, cetearyl alcohol polyoxyethylene (12) ether, cetearyl alcohol polyoxyethylene (20) ether, cetearyl Cetyl Caprylate, Cetyl Polyoxyethylene (20) Ether, Cetyl Polyoxyethylene (24) Ether, Cetyl Acetate, Cetyl Caprylate, Cetyl Palmitate, Chamomile ( anthemis nobilis) oil, cholesterol, cholesteryl esters, cholesteryl hydroxystearate, citric acid, sage (salvia sclarea) oil, cocoa (theobroma cacao) butter, coco-caprylate/caprate, coconut (cocos nucifera) oil, collagen, collagen amino acids, corn (zea mays) oil, fatty acids, decyl oleate, dimethicone copolyol, dimethiconol, adipic acid Dicapryl Ester, Dicaprylyl Succinate, Dipentaerythritol Hexacaprylate/Hexacaprate, DNA, Erythritol, Ethoxylated Diethylene Glycol, Ethyl Linoleate, Eucalyptus Eucalyptus, Moongum Grass (oenothera biennis) oil, fatty acids, spotted geranium oil, glucosamine, glucoglutamate, glutamic acid, glyceryl polyoxyethylene (26) ether, glycerin, glycerol, glyceryl distearate , Glyceryl Hydroxystearate, Glyceryl Laurate, Glyceryl Linoleate, Glyceryl Myristate, Glyceryl Oleate, Glyceryl Stearate, Glyceryl Stearate SE, Glycine, Glycol Stearate Esters, Glycol Stearate SE, Glucosaminoglycan, Grape (vitis vinifera) Seed Oil, American Hazel (Corylus americana) Nut Oil, European Hazel (Corylus Avellana) Nut Oil, Hexylene Glycol, Hyaluronic Acid Acid, Safflower (carthamus tinctorius) Oil, Hydrogenated Castor Oil, Hydrogenated Glyceryl Cocoate, Hydrogenated Coconut Oil, Hydrogenated Lanolin, Hydrogenated Lecithin, Hydrogenated Palm Glycerides, Hydrogenated Palm Kernel Oil, Hydrogenated Soybean Oil, Hydrogenated Tallow Glycerides, Hydrogenated Vegetable Oil, Hydrolyzed Collagen, Hydrolyzed Elastin, Hydrolyzed Glucosaminoglycan, Hydrolyzed Keratin, Hydrolyzed Soy Protein, Hydroxylated Lanolin, Hydroxyproline, Isocetyl Stearate, Isocetyl Stearoyl Stearate, Isoleic Acid Decyl ester, isopropyl isostearate, isopropyl lanolate, isopropyl myristate, isopropyl palmitate, isopropyl stearate, isostearamide DEA, isostearic acid, isopropyl Stearyl lactate, isostearyl pivalate, jasmine (jasminum officinale) oil, jojoba (buxus chinensis) oil, macroalgae, chestnut (aleurites moluccana) nut oil, lactamide MEA, lanolin alcohol polyol Oxyethylene (16) ether, lanolin alcohol polyoxyethylene (10) ether acetate, lanolin, lanolin acid, lanolin alcohol, lanolin oil, lanolin wax, lavender (lavandula angustifolia) oil, lecithin, lemon (citrus medicalimonum) oil, linoleic acid, linolenic acid, macadamia nut oil, maltitol, chamomilla recutita oil, methyl glucose sesquistearate, methylsilanol PCA, mineral oil, mink oil , Mortierella Oil, Myristyl Lactate, Myristyl Myristate, Myristyl Propionate, Neopentyl Glycol Dicaprylate/Dicaprate, Capryl Lauryl Alcohol, Capryl Lauryl Alcohol Alcohol myristate, octyl lauryl stearyl stearate, octyl hydroxystearate, octyl palmitate, octyl salicylate, octyl stearate, oleic acid, olive (oleaeuropaea) oil, orange (citrus aurantium dulcis) oil, palm (elaeis guineensis) oil, palmitic acid, pantethine, panthenol, panthenyl ethyl ether, paraffin, PCA, peach (prunus persica) kernel oil, peanut (arachishypogaea) oil, PEG- 8C12-18 Esters, PEG-15 Coco Alkylamine, PEG-150 Distearate, PEG-60 Glyceryl Isostearate, PEG-5 Glyceryl Stearate, PEG-30 Glyceryl Stearate , PEG-7 Hydrogenated Castor Oil, PEG-40 Hydrogenated Castor Oil, PEG-60 Hydrogenated Castor Oil, PEG-20 Methyl Glucose Sesquistearate, PEG40 Sorbitan Full Oleate, PEG-5 Soybean Sterols, PEG-10 Soy Sterol, PEG-2 Stearate, PEG-8 Stearate, PEG-20 Stearate, PEG-32 Stearate, PEG-40 Stearate, PEG- 50 Stearate, PEG-100 Stearate, PEG-150 Stearate, Pentadecalactone, Mentha Piperita Oil, Vaseline Lin, phospholipids, polyamino acid polysaccharide condensate, polyglyceryl-3 diisostearate, polyquaternium-24, polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80. Polysorbate 85, Potassium Myristate, Potassium Palmitate, Propylene Glycol, Propylene Glycol Dicaprylate/Dicaprate, Propylene Glycol Dicaprylate, Propylene Glycol Dinonanoate, Propylene Glycol Laurate, Propylene Glycol Stearate Esters, Propylene Glycol Stearate SE, PVP, Pyridoxine Dipalmitate, Retinol, Retinyl Palmitate, Rice (oryza sativa) Bran Oil, RNA, Rosemary (rosmarinus officinalis) Oil, Rosemary Oil, safflower (carthamus tinctorius) oil, sage (salvia officinalis) oil, sandalwood (santalum album) oil, serine, serum albumin, sesame (sesamum indicum) oil, shea butter (butyrospermum parkii) butter, silk powder, cartilage Sodium sulfate, sodium hyaluronate, sodium lactate, sodium palmitate, sodium PCA, sodium polyglutamate, soluble collagen, sorbitan laurate, sorbitan oleate, sorbitan palmitate Esters, sorbitan sesquioleate, sorbitan stearate, sorbitol, soybean (glycine soja) oil, sphingolipids, squalane, squalene, stearamide MEA-stearic acid Esters, Stearic Acid, Stearyl Dimethicone, Stearyl Trimethylsilane, Stearyl Alcohol, Stearyl Glycyrrhetinate, Stearyl Heptanoate, Stearyl Stearyl Fatty acid esters, sunflower (helianthus annuus) seed oil, sweet almond (prunus amygdalus dulcis) oil, synthetic beeswax, tocopherol, tocopheryl acetate, tocopheryl linoleate, tribehenin, tridecyl pivalate , Tridecyl Stearate, Triethanolamine, Tristearin, Urea, Vegetable Oil, Water, Wax, Wheat (triticum vulgare) Germ Oil, and Ylang-ylang (Cananga odorata) Oil.

c.Antioxidant

Non-limiting examples of antioxidants that can be used with the compositions disclosed herein include acetylcysteine, ascorbyl polypeptide, ascorbyl dipalmitate, ascorbyl methylsilanol pectate, ascorbyl palmitate, ascorbyl hard Fatty acid esters, BHA, BHT, tert-butyl hydroquinone, cysteine, cysteine HCI, dipentyl hydroquinone, di-tert-butyl hydroquinone, dicetyl thiodipropionate, dioleyl tocopherol Methylsilanol, Disodium Ascorbyl Sulfate, Distearyl Thiodipropionate, Ditridecyl Thiodipropionate, Lauryl Gallate, Erythorbic Acid, Ascorbyl Ethyl Ferulate, Ethyl Ferulate , ferulic acid, gallate, hydroquinone, isooctyl thioglycolate, kojic acid, magnesium ascorbate, magnesium ascorbyl phosphate, methylsilanol ascorbate, natural plant antioxidants such as green tea or grape seed extract, normethyl Dihydroguaiaretic acid, octyl gallate, phenylthioglycolic acid, potassium ascorbate tocopheryl phosphate, potassium sulfite, propyl gallate, quinone, rosmarinic acid, sodium ascorbate, sodium bisulfite, sodium erythorbate , sodium metabisulfite, sodium sulfite, superoxide dismutase, sodium thioglycolate, sorbitol furfural, thiodiglycol, thiodiacetamide, thiodiacetic acid, thioglycolic acid, thiolactic acid, thiowater Salicylic Acid, Tocopheryl Polyoxyethylene Ether-5, Tocopheryl Polyoxyethylene Ether-10, Tocopheryl Polyoxyethylene Ether-12, Tocopheryl Polyoxyethylene Ether-18, Tocopheryl Polyoxyethylene Ether-50, Tocopherol , tocosolan, tocopheryl acetate, tocopheryl linoleate, tocopheryl nicotinate, tocopheryl succinate, and tris(nonylphenol) phosphite.

d. Structuring agent

In other non-limiting aspects, the compositions disclosed herein can include a structuring agent. In certain aspects, the structuring agent helps to provide rheological characteristics to the composition to aid in the stability of the composition. In other aspects, the structuring agent can also function as an emulsifier or surfactant. Non-limiting examples of structuring agents include stearic acid, palmitic acid, stearyl alcohol, cetyl alcohol, behenyl alcohol, stearic acid, palmitic acid, stearic acid having an average of about 1 to about 21 ethylene oxide units. Polyglycol ethers of fatty alcohols, polyglycol ethers of cetyl alcohol having an average of about 1 to about 5 ethylene oxide units, and mixtures thereof.

e. Emulsifier

In some aspects of the invention, the composition contains no emulsifiers. In other aspects, however, the composition may contain one or more emulsifiers. Emulsifiers can reduce the surface tension between phases and improve the formulation and stability of emulsions. Emulsifiers can be nonionic, cationic, anionic, and zwitterionic emulsifiers (see McCutcheon's (1986); US Patent Nos. 5,011,681; 4,421,769; 3,755,560). Non-limiting examples include glycerol esters, propylene glycol esters, fatty acid esters of propylene glycol, fatty acid esters of polypropylene glycol, esters of sorbitol, sorbitan esters, carboxylic acid copolymers, esters and ethers of glucose, ethoxylated Esters, ethoxylated alcohols, alkyl phosphates, polyoxyethylene fatty ether phosphates, fatty acid amides, acyl lactylates, fatty acid salts, TEA stearate, DEA oleyl polyoxyethylene ether- 3 phosphate esters, macrogol 20 sorbitan monolaurate (polysorbate 20), macrogol 5 soy sterol, stearyl polyoxyethylene ether-2, stearyl polyoxyethylene ether -20, stearyl polyoxyethylene ether-21, cetearyl polyoxyethylene ether-20, cetearyl glucoside, cetearyl alcohol, C12-C13 pareth-3, PPG-2 Methyl Glucose Ether Distearate, PPG-5-Ceteth-20, Bis-PEG/PPG-20/20 Dimethicone, Cetyl Polyoxyethylene Ether- 10. Polysorbate 80, cetyl phosphate, potassium cetyl phosphate, diethanolamine cetyl phosphate, polysorbate 60, glyceryl stearate, PEG-100 stearate, peanut Alcohol, Arachidyl Glucoside and mixtures thereof.

f. Silicone-containing compounds

In a non-limiting aspect, silicone-containing compounds include any member of the family of polymeric products in which the molecular backbone consists of alternating silicon and oxygen atoms with side groups attached to the silicon atoms. By varying the length of the -Si-O- chain, side groups, and crosslinks, silicones can be synthesized into a wide variety of materials. Their consistency can vary from liquid to gel to solid.

Silicone-containing compounds that may be used in the context of the present invention include those described in this specification or known to those of ordinary skill in the art. Non-limiting examples include silicone oils (eg, volatile and non-volatile oils), gels and solids. In particular aspects, silicone-containing compounds include silicone oils, such as polyorganosiloxanes. Non-limiting examples of organopolysiloxanes include dimethicone, cyclomethicone, polysiloxane-11, phenyl trimethicone, trimethicone Aminodimethylsiloxane, stearoxytrimethylsilane or mixtures thereof and other organosiloxane materials in any given proportion to suit the intended application (e.g., for specific areas such as skin, hair or eyes) to the desired consistency and application profile. "Volatile silicone oils" include silicone oils that have a low heat of vaporization, such as typically less than about 50 calories per gram of silicone oil. Non-limiting examples of volatile silicone oils include cyclomethicones such as Dow Corning 344 Fluid, Dow Corning 345 Fluid, Dow Corning 244 Fluid, and Dow Corning 245 Fluid, Volatile Silicone 7207 (Union Carbide, Danbury, Conn. company); low viscosity dimethicones, such as those having a viscosity of about 50 cst or less (for example, dimethicones such as Dow Corning 200-0.5 cst fluid) . Dow Corning Fluid is commercially available from Dow Corning Corporation, Midland, Michigan. In the third edition of the CTFA Cosmetic Ingredient Dictionary (incorporated by reference), cyclomethicone and dimethicone are described as cyclomethicone compounds and trimethicone compounds, respectively. A mixture of fully methylated linear siloxanes terminated with methylsiloxy units. Other non-limiting volatile silicone oils that can be used in the context of the present invention include those available from General Electric Co., Silicone Products Div. of Waterford, NY and SWS Silicones Div. of Stauffer Chemical Co. of Adrian, Michigan. earned those.

g. Essential oils

Essential oils include oils from herbs, flowers, trees, and other plants. Such oils generally exist as tiny droplets between plant cells and can be extracted by several methods known to those skilled in the art (e.g., steam distillation, floral extraction (e.g., using fat), maceration, solvent extraction or mechanical pressing). These types of oils tend to evaporate when exposed to air (eg, volatile oils). Therefore, although many essential oils are colorless, over time they oxidize and become darker in color. Essential oils are insoluble in water but soluble in alcohols, ethers, fixed oils (vegetable) and other organic solvents. General physical characteristics found in essential oils include boiling points ranging from about 160°C to 240°C and densities ranging from about 0.759 to about 1.096.

Essential oils are generally named by the plant from which the oil is found. For example, rose oil or peppermint oil come from rose or peppermint plants, respectively. Non-limiting examples of essential oils that may be used in the context of the present invention include sesame oil, macadamia oil, tea tree oil, evening primrose oil, Spanish sage oil, Spanish rosemary oil, coriander oil, thyme oil, allspice Oil, Rose Oil, Anise Oil, Balsam Oil, Bergamot Oil, Rosewood Oil, Cedar Oil, Chamomile Oil, Clary Sage Oil, Clary Sage Oil, Clove Oil, Cedarwood Oil, Eucalyptus Oil, Fennel Oil , Sea Fennel Oil, Frankincense Oil, Geranium Oil, Ginger Oil, Grapefruit Oil, Jasmine Oil, Juniper Oil, Lavender Oil, Lemon Oil, Lemongrass Oil, Pear Berry Oil, Orange Oil, Oregano Oil, Myrrh Oil, Neroli Oil, Orange Oil, Green Leaf Oil, Pepper Oil, Black Pepper Oil, Petitgrain Oil, Pine Oil, Rose Otto Oil, Rosemary Oil, Sandalwood Oil, Spearmint Oil, Nardine Oil, Vetiver Herb Oil, Oil of Wintergreen, Ylang Ylang. Other essential oils known to those skilled in the art are also contemplated to be useful in the context of the present invention.

h.Thickener

Thickeners, including thickeners or gelling agents, include substances that increase the viscosity of a composition. Thickeners include those that can increase the viscosity of a composition without substantially altering the efficacy of the active ingredients within the composition. Thickeners can also increase the stability of the compositions disclosed herein. In some aspects disclosed herein, the thickener includes hydrogenated polyisobutylene, trihydroxystearin, ammonium acryloyldimethyltaurate/VP copolymer, or mixtures thereof.

Non-limiting examples of additional thickeners that may be used in the context of the present invention include carboxylic acid polymers, cross-linked polyacrylate polymers, polyacrylamide polymers, polysaccharides, and gums. Examples of carboxylic acid polymers include crosslinked compounds containing one or more monomers derived from acrylic acid, substituted acrylic acids, and salts and esters of these acrylic acids and substituted acrylic acids, wherein the crosslinking agent contains two or More carbon-carbon double bonds and derived from polyols (see US Patent Nos. 5,087,445; 4,509,949; 2,798,053; CTFA International Cosmetic Ingredient Dictionary, Fourth Edition, 1991, pp. 12 and 80). Examples of commercially available carboxylic acid polymers include carbomers, which are homopolymers of acrylic acid crosslinked with allyl ethers of sucrose or pentaerythritol (eg, the Carbopol ^™ 900 series from BF Goodrich).

Non-limiting examples of crosslinked polyacrylate polymers include cationic and nonionic polymers. Examples are described in US Patent Nos. 5,100,660; 4,849,484; 4,835,206; 4,628,078; 4,599,379.

Non-limiting examples of polyacrylamide polymers (including nonionic polyacrylamide polymers, including substituted branched or unbranched polymers) include polyacrylamides, isoparaffins, and polyoxyethylene lauryl ( 7) Multi-block copolymers of ether, acrylamide and acrylamide substituted with acrylic acid and substituted acrylic acid.

Non-limiting examples of polysaccharides include cellulose, carboxymethyl hydroxyethyl cellulose, cellulose acetate propionate carboxylate, hydroxyethyl cellulose, hydroxyethyl ethyl cellulose, hydroxypropyl cellulose, hydroxypropyl Methylcellulose, methylhydroxyethylcellulose, microcrystalline cellulose, sodium cellulose sulfate and mixtures thereof. Other examples are alkyl-substituted celluloses, where the hydroxyl groups of the cellulose polymer are hydroxyalkylated (preferably hydroxyethylated or hydroxypropylated) to form hydroxyalkylated cellulose, which is then treated with C10- C30 linear or branched alkyl groups are further modified by ether linkages. Typically these polymers are ethers of C10-C30 linear or branched chain alcohols and hydroxyalkylcelluloses. Other useful polysaccharides include scleroglucans comprising linear chains of (1-3) linked glucose units with one (1-6) linked glucose every three units.

Non-limiting examples of gums that may be used in the present invention include gum arabic, agar, algin, alginic acid, ammonium alginate, branched starch, calcium alginate, calcium carrageenan, carnitine, carrageenan, Dextrin, Gelatin, Gellan Gum, Guar Gum, Guar Hydroxypropyltrimonium Chloride, Hectorite, Hyaluronic Acid, Hydrated Silica, Hydroxypropyl Chitosan, Hydroxypropyl Guar Gum, Caraya Gum, Macroalgae, Carob Bean Gum, Natto Gum, Potassium Alginate, Potassium Carrageenan, Propylene Glycol Alginate, Sclerotin Gum, Sodium Carboxymethyl Dextran, Carrageenan Sodium gum, tragacanth gum, xanthan gum and mixtures thereof.

i. Preservatives

Non-limiting examples of preservatives that may be used in the context of the present invention include quaternary ammonium preservatives such as polyquaternium-1 and benzalkonium halides such as benzalkonium chloride ("BAC") and benzalkonium bromide)), parabens (such as methylparaben and propylparaben), phenoxyethanol, benzyl alcohol, chlorobutanol, phenol, sorbic acid, thimerosal, or combinations thereof.

2. Drug ingredients

Pharmaceutically active ingredients are also contemplated to be useful with the compositions disclosed herein. Non-limiting examples of pharmaceutical active ingredients include anti-acne agents, agents for the management of rosacea, analgesics, anesthetics, anorectal, antihistamines, anti-inflammatory agents including non-steroidal anti-inflammatory drugs, antibiotics, Antibacterial agents, antiviral agents, antimicrobial agents, anticancer actives, antiscarcetic agents, pediculicides, antineoplastic agents, antiperspirants, antipruritic agents, antipsoriatic agents, antiseborrheic agents, biologically active proteins and Polypeptides, burn treatment agents, cauterization agents, depigmentants, depilatory agents, diaper rash treatment agents, enzymes, hair growth stimulators, hair growth inhibitors including DFMO and its salts and the like, hemostatic agents, keratolytic agents, Aphthous Treatments, Cold Sores Treatments, Dental or Periodontal Treatments, Photosensitizing Actives, Skin Protectants/Barriers, Steroids Including Hormones and Corticosteroids, Sunburn Treatments, Sunscreens, Transdermal Actives, Nasal Actives, vaginal actives, wart treatment agents, wound treatment agents, wound healing agents, etc.

F. Kit

Kits are also contemplated for use with certain aspects of the invention. For example, compositions disclosed herein can be included in kits. A kit can include a container. Containers may include bottles, metal tubes, laminated tubes, plastic tubes, dispensers, pressurized containers, barrier containers, packs, compartments, lipstick containers, compress containers, cosmetic trays capable of holding cosmetic compositions, or other types of containers, such as injectable or blow molded plastic containers in which to hold the dispersion or composition or the desired bottle, dispenser or package. The kit and/or container may comprise indicia on its surface. For example, a marker can be a word, phrase, abbreviation, picture or symbol.

The container can dispense a predetermined amount of the composition. In other embodiments, squeeze containers (eg, metal, laminate or plastic tubes) can be used to dispense the desired amount of the composition. Compositions can be dispensed as a spray, aerosol, liquid, fluid or semi-solid. Containers can have spray, suction or squeeze mechanisms. The kit can also comprise instructions for using the kit components as well as for using any compositions contained in the container. The instructions can include an explanation of how to administer, use and store the composition.

Example

The following examples serve to illustrate some embodiments/examples of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques determined by the inventors to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

Example 1

A formulation with ingredients from Example 1 was prepared as a facial mask. The formulations in Table 1 were prepared as exfoliating masks. The formulations in Table 2 were prepared as repairing or exfoliating masks.

Table 1

**Excipients are added to modify the rheology of the composition. Alternatively, the amount of water may vary so long as the amount of water in the composition is at least 40% by weight, preferably 45% to 65% by weight.

Table 2

**Excipients are added to modify the rheology of the composition. Alternatively, the amount of water may vary so long as the amount of water in the composition is at least 15%, preferably 25% to 85% by weight.

Example 2

Increases involucrin present in epidermal keratinocytes:

Involucrin is a differentiation protein that plays an important role in skin regeneration. ELISA was used to analyze the effect of components on involucrin production by human epidermal keratinocytes. Disclosed herein are Mucor miehei extracts (ACTIZYME ^™ ), sugar isomers (Benoiderm), Bacillus fermentation products (KERATOLINE ^™ ), and nopal cactus flower extracts ( EXFOLACTIVE ^™ ) increased the involucrin content of human epidermal keratinocytes (Table 3).

table 3

Element concentration Increased involucrin % ACTIZYME ^TM (Mucorella miehei Extract) 2% 7.7% Benoiderm (sugar isomers) 1% 108% KERATOLINE ^TM (Bacillus ferment product) 2% 84% EXFOLACTIVE ^TM (Nopal Cactus Flower Extract) 4% 84%

Briefly, subconfluent normal human mature keratinocytes (Cascade Biologics) were cultured in EpiLife Standard Growth Medium (Cascade Biologics) at 37°C in 5% _CO2 using the active ingredients, combinations of ingredients disclosed in the specification or any of the compositions with said combination for 6 hours. After incubation, cells are washed, harvested and lysed. The amount of involucrin in cell lysates can be quantified using a sandwich enzyme-linked immunosorbent assay (ELISA).

The endpoint of the assay can be a spectrophotometric measurement that reflects the presence of involucrin and cell viability. The involucrin endpoint assay employs a quantitative sandwich enzyme immunoassay technique, by virtue of which involucrin-specific monoclonal antibodies have been pre-coated on microwell plates. Standards and samples can be pipetted into the wells and any involucrin present is bound by the immobilized antibodies. After washing away any unbound material, involucrin-specific enzyme-linked polyclonal antibodies can be added to the wells. After washing to remove all unbound abzyme reactants, substrate solution can be added to the wells, and the color developed is proportional to the amount of involucrin bound in the initial step, detected at 450 nm using a microplate reader, and can be stopped. color and can measure the intensity of the color.

Example 3

Clinical increase in skin regeneration/renewal/exfoliation

Increased skin regeneration/renewal/exfoliation can reduce the signs of aging and improve the skin's visual appearance. In a clinical study, a combination of Mucor miehei extract (ACTIZYME ^TM ), Bacillus ferment product (KERATOLINE ^TM ) and nopal cactus flower extract (EXFOLACTIVE ^TM ) Each of the combination of Mucor extract (ACTIZYME ^™ ), sugar isomers (Benoiderm) and Bacillus ferment product (KERATOLINE ^™ ) reduced the mean time to skin regeneration/renewal/exfoliation from 21.40 days to less than 14 days (Table 4).

Table 4

Briefly, the regenerating/renewing/exfoliating effects of two combinations of ingredients were evaluated on the skin of 26 healthy adult female volunteers using DHA (dihydroxyacetone)-induced skin pigmentation. DHA was applied to the treated area on the volunteer's skin. No treatment, a combination of 2% ACTIZYME ^TM (Mucorella miehei extract), 4% EXFOLACTIVE ^TM (Nopal cactus flower extract) and 2% KERATOLINE ^TM (Bacillus ferment product), or 2% ACTIZYME ^TM (Mich Mucor extract), 1% Benoiderm (isomers of sugars) and 2% KERATOLINE ^™ (Bacillus fermentation product) in combination were applied to the treated areas over a period of 2 weeks. Colorimetric measurements of the treated areas were taken during the baseline period before DHA gel application, after DHA gel application and at the end of the treatment period. For each treatment, the number of days until the treated area of skin returned to baseline colorimetric readings was determined. Both combinations showed a significant reduction in days of skin regeneration/renewal/skin exfoliation compared to untreated skin.

Example 4

(additional analysis)

The efficacy of any of the ingredients disclosed throughout the specification and claims, or any combination of ingredients or compositions having combinations of said ingredients, can be determined by methods known to those of ordinary skill in the art. The following are non-limiting ones that can be used in the context of the present invention. It should be appreciated that other testing procedures may be used including, for example, objective and subjective procedures.

B16 Pigmentation Analysis: Melanogenesis is the process by which melanocytes produce melanin, the naturally occurring pigment that gives skin, hair, and eyes their color. Inhibiting melanogenesis is beneficial in preventing skin darkening and lightening dark spots associated with ageing. This bioassay employs B16-F1 melanocytes (ATCC), an immortalized mouse melanoma cell line, to analyze the effect of compounds on melanin production. The end point of the assay is the spectrophotometric measurement of melanin production and cell viability. B16-F1 melanocytes can be cultured in standard DMEM growth medium with 10% fetal bovine serum (Mediatech) at 37°C in 10% CO ₂ , and then treated with the active ingredients, ingredients disclosed in this specification The combination or any of the compositions with the combination was treated for 6 days. After incubation, cell viability was quantified by measuring melanin secretion by absorbance at 405 nm.

Collagen Stimulation Assay: Collagen is an extracellular matrix protein critical to skin structure. Increased collagen synthesis helps improve skin firmness and elasticity. This bioassay can be used to examine the effect of any of the active ingredients disclosed in this specification, combinations of ingredients, or compositions having said combinations, on the production of procollagen peptides (precursors of collagen) by human epidermal fibroblasts. The endpoint of this assay is a spectrophotometric measurement reflecting the presence of procollagen peptides and cell viability. The assay employs a quantitative sandwich enzyme immunoassay technique whereby monoclonal antibodies specific for procollagen peptides are pre-coated on microwell plates. Standards and samples can be pipetted into the wells and any procollagen peptides present are bound by the immobilized antibodies. After all unbound material has been washed away, an enzyme-linked polyclonal antibody specific for the procollagen peptide is added to the wells. After washing to remove any unbound antibody-enzyme reaction, substrate solution can be added to the wells and the color developed proportional to the amount of procollagen peptide bound in the initial step, detected at 450 nm using a microplate reader. Color development can be stopped and the intensity of the color measured. Subconfluent normal human mature epidermal fibroblasts (Cascade Biologics) cultured in standard DMEM growth medium with 10% fetal bovine serum (Mediatech) in 10% _CO at 37°C can be cultured using the methods described in this specification. Each of the disclosed combinations of ingredients or compositions with said combinations was treated for 3 days. After culturing, the cell culture medium can be collected and the amount of procollagen peptide secretion can be quantified using Takara's sandwich enzyme-linked immunosorbent assay (ELISA) (#MK101).

Tumor necrosis factor-α (TNF-α) analysis: TNF-α, the prototypical ligand of the TNF superfamily, is a pleiotropic cytokine that plays a major role in inflammation. An increase in its expression is associated with an upregulation of pro-inflammatory activity. The bioassay can be used to detect the effect of any one of the active ingredients, the combination of ingredients or the composition having the combination disclosed in this specification on the production of TNF-α by human epidermal keratinocytes.

Phorbol-12-myristate-13-acetate (PMA, 10 ng/ml, Sigma Chemical, #P1585-1MG) may be used with or with the active ingredient, combination of ingredients disclosed in this specification Subconfluent normal human mature keratinocytes (Cascade Biologics) cultured in EpiLife standard growth medium (Cascade Biologics) in 5% CO ₂ at 37° C. for 6 hours were treated with either of the compositions. It has been shown that PMA leads to a marked increase in TNF-α secretion which peaks after 6 hours of treatment. After incubation, the cell culture medium can be harvested and the amount of TNF-α secretion quantified using a sandwich enzyme-linked immunosorbent assay (ELISA) from R&D Systems (#DTA00C).

The endpoint of this assay may be a spectrophotometric measurement reflecting the presence of TNF-α and cellular viability. The TNF-α endpoint assay uses a quantitative sandwich enzyme immunoassay technique, so monoclonal antibodies specific for TNF-α have been pre-coated on microwell plates. Standards and samples can be pipetted into the wells and any TNF-α present is bound by the immobilized antibodies. After all unbound material has been washed away, an enzyme-linked polyclonal antibody specific for TNF-α can be added to the wells. After washing to remove any unbound antibody-enzyme reaction, substrate solution can be added to the wells, and the color developed is proportional to the amount of TNF-α bound in the initial step, detected at 450 nm using a microplate reader. Color development can be stopped and the intensity of the color measured.

Antioxidant (AO) Assay: An in vitro bioassay measures the total antioxidant capacity of any of the ingredients, combinations of ingredients, or compositions having said combinations disclosed in this specification. The assay relies on the ability of antioxidants in the sample to inhibit (2,2'-azino-bis-[3-ethylbenzothiazoline sulfonate]) is oxidized by metmyoglobin to The antioxidant system of organisms consists of enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase; macromolecules, such as albumin, ceruloplasmin, and ferritin; and numerous small molecules, Includes ascorbic acid, alpha-tocopherol, beta-carotene, reduced glutathione, uric acid and bilirubin. The sum of endogenous food-derived antioxidants represents the total antioxidant capacity of extracellular fluid. The synergy of all the different antioxidants provides greater protection against attack by reactive oxygen or nitrogen radicals than any single compound alone. Therefore, the total antioxidant capacity may give more relevant biological information than that obtained by measuring the individual components, since it takes into account the cumulative effect of all antioxidants present in plasma and body fluids. The ability of the antioxidants in the samples to prevent oxidation of ABTS was compared to that of Trolox, a water-soluble analogue of tocopherol, and quantified as molar equivalents of Trolox. Antioxidant Potency Kit #709001 from Cayman Chemical (Ann Arbor, Mich., USA) can be used as a measure of total In vitro bioassay of antioxidant capacity. Protocols can be performed according to the manufacturer's recommendations. The assay relies on antioxidants in the sample to inhibit (2,2'-azino-bis-[3-ethylbenzothiazoline sulfonate]) is oxidized by metmyoglobin to The ability of the antioxidants in the sample to prevent oxidation of ABTS can be compared to the ability of Trolox, a water-soluble analogue of tocopherol, and quantified as molar equivalents of Trolox.

ORAC Analysis: The oxygen radical absorption (or absorbance) capacity (ORAC) of any of the active ingredients disclosed in this specification, combinations of ingredients, or compositions having said combinations can also be measured by measuring the Antioxidant activity was analyzed. The assay can quantify the degree and time for which activity of oxidants, such as oxygen free radicals known to cause damage to cells such as skin cells, is inhibited. ORAC values for any of the active ingredients disclosed in this specification, combinations of ingredients, or compositions having said combinations can be determined by methods known to those of ordinary skill in the art (see U.S. Publication Nos. 2004/0109905 and 2005/ 0163880; Cao et al. (1993), the entire contents of which are incorporated by reference). In summary, the assay described by Cao et al. (1993) measures the ability of antioxidant compounds to inhibit the decrease in B-phycoerythrin fluorescence (B-PE) induced by the hydroperoxy radical generator AAPH in test materials.

Mushroom tyrosinase activity assay: In mammalian cells, tyrosinase catalyzes two steps in the biosynthesis of melanin from tyrosine (and from dopachrome polymerization). Tyrosinase is located in melanocytes and produces melanin (aromatic quinone compound) that gives skin, hair, and eye color. Mushroom tyrosinase (Sigma) can be utilized with its substrate L-Dopa in the presence or absence of any one of the active ingredients disclosed in this specification, a combination of ingredients, or a combination having said combinations. (Fisher) Culture. Pigmentation can be assessed by colorimetric plate reading at 490nm. The percent inhibition of mushroom tyrosinase activity can be calculated by comparison to an untreated control to determine the ability of the test ingredients or combinations thereof to inhibit the activity of the purified enzyme. The inhibition of the test extracts was compared to that of kojic acid (Sigma).

Matrix metalloproteinase activity (MMP3; MMP9) assay: Matrix metalloproteinase (MMP) inhibition assay in vitro. MMPs are extracellular proteases that, by virtue of their broad substrate specificity, function in many normal and disease states. MMP3 substrates include collagen, fibronectin, and laminin; while MMP9 substrates include collagen VII, fibronectin, and laminin. Using the Colorimetric Drug Discovery Kit for MMP3 (AK-400) and MMP-9 (AK-410) from BioMol International, the assay was designed to measure the protease activity of MMPs using a sulfur-containing polypeptide as a chromogenic substrate (Ac- PLG-[2-Mercapto-4-methyl-pentanoyl]-LG-OC2H5)5,6. The peptide bond at the MMP cleavage site is replaced by a thioester bond in the sulfur-containing polypeptide. This bond is hydrolyzed by MMP to produce a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to generate 2-nitro-5-thiobenzoic acid, which can Detection is by its absorbance at 412 nm (ε=13600 M-1 cm-1 at pH 6.0 and above 7). Any of the active ingredients, combinations of ingredients, or compositions having said combinations disclosed in this specification can be assayed.

Cyclooxygenase (COX) assay: In vitro cyclooxygenase-1 and -2 (COX-1, -2) inhibition assay. COX is a bifunctional enzyme that exhibits both cyclooxygenase and peroxidase activities. Cyclooxygenase activity converts arachidonic acid to hydroperoxide endoperoxide (prostaglandin G2; PGG2), and the peroxidase component reduces endoperoxide (prostaglandin H2; PGH2) to the corresponding alcohol , a precursor of prostaglandins, thromboxanes, and cycloprostaglandins. This COX inhibition screening assay measures the peroxidase component of cyclooxygenase. Peroxidase activity was analyzed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD). The inhibition screening assay included both COX-1 and COX-2 enzymes to screen for isozyme-specific inhibitors. The colorimetric COX (sheep) inhibitor screening assay (#760111, Cayman Chemical) can be used to analyze the effect of each of the active ingredients disclosed in this specification, the combination of ingredients, or the composition having said combination on purified epoxy Effect of synthase (COX-1 or COX-2). Purified enzyme, heme, and assay extracts can be mixed in assay buffer and incubated for 15 min at room temperature with shaking according to the manufacturer's instructions. Following incubation, arachidonic acid and a colorimetric substrate can be added to initiate the reaction. Color progression can be assessed by color guide plate reading at 590nm. The percent inhibition of COX-1 or COX-2 activity can be calculated by comparison to untreated controls to determine the ability of the test extract to inhibit the activity of the purified enzyme.

Lipoxygenase (LO) Assay: In vitro lipoxygenase inhibition assay. LO is a non-heme iron-containing dioxygenase that catalyzes the addition of molecular oxygen to fatty acids. Linoleate and arachidonate are the main substrates of LO in plants and animals. Arachidonic acid can then be converted to hydroxyeicotranenoic (HETE) acid derivatives, which are subsequently converted to leukotrienes, potent mediators of inflammation. This assay provides an accurate and convenient assay for screening lipoxygenase inhibitors by measuring hydroperoxide production from lipoxygenase (5-, 12-, or 15-LO) incubated with arachidonic acid. method. The colorimetric LO Inhibitor Screening Kit (#760700, Cayman Chemical) can be used to determine the inhibition of any one of the active ingredients disclosed in this specification, the combination of ingredients, or a composition having said combination. active ability. Purified 15-lipoxygenase and test components can be mixed in assay buffer and incubated with shaking for 10 minutes at room temperature. After incubation, arachidonic acid can be added to initiate the reaction and the mixture is incubated for an additional 10 minutes at room temperature. A colorimetric substrate can be added to stop catalysis and color progression assessed by fluorescence plate reading at 490nm. The percent inhibition of lipoxygenase activity can be assessed as compared to an untreated control to determine any of the active ingredients disclosed in this specification, combinations of ingredients, or compositions having said combinations The ability to inhibit the activity of purified enzymes.

Elastase analysis: Molecular Probes (Eugene, Oregon, USA) can be used Elastase Assay (Kit #E-12056) as an in vitro assay for measuring inhibition of elastase activity by each of the active ingredients disclosed in this specification, a combination of ingredients, or a composition having said combination. Enzyme Inhibition Assay. The EnzChek kit contains soluble bovine cervical ligament elastin, which can be labeled with a dye to enable quenching of conjugated fluorescence. Non-fluorescent substrates can be digested with elastase or other proteases to generate highly fluorescent fragments. The resulting fluorescence enhancement can be monitored using a fluorescence microplate reader. The digestion product from the elastin substrate has an absorption maximum at about 505 nm and a fluorescence emission maximum at about 515 nm. When using the EnzChek Elastase Assay Kit to screen for elastase inhibitors, the peptide, chloromethyl ketone, can be used as a selective, common elastase inhibitor.

Oil Control Assay: Assays for measuring the reduction in sebum secretion in the sebaceous glands and/or the reduction in sebum production in the sebaceous glands can be performed using standard techniques known to those of ordinary skill in the art. In a specific example, the forehead can be used. Each of the active ingredients disclosed in this specification, the combination of ingredients, or any of the compositions having the combination can be applied to a portion of the forehead once or twice a day for a fixed period of days (eg, 1, 2 , 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or more days), while the rest of the forehead is not treated with the composition. After expiration of a fixed period of days, sebum secretion can be analyzed by applying fine blotting paper to treated and untreated forehead skin. This is done by first removing all the sebum from the treated and untreated areas with wet and dry cloths. The blotter is then applied to the treated and untreated forehead area, and a rubber band can be placed around the forehead to gently press the blotter against the skin. After 2 hours, the blotter paper can be removed, allowed to dry, and transilluminated. Darker blotters correspond to more sebum production (or lighter blotters correspond to reduced sebum production.

Erythema Assay: An assay that measures reduction in skin redness can be assessed using the Minolta Chromometer. Skin erythema can be induced by applying a 0.2% sodium lauryl sulfate solution to the subject's forearm. The area is protected for 24 hours with an occlusive patch. After 24 hours, the patch is removed and irritation-induced redness can be assessed using the a ^* value of the MinoltaChroma Meter. The a ^* value measures the change in skin color in the red area. Immediately following the reading, the area is treated with any of the active ingredients, combinations of ingredients, or compositions having said combinations disclosed in this specification. Repeat measurements can be taken at regular intervals to determine the ability of the formulation to reduce redness and irritation.

Skin Moisture/Hydration Analysis: The benefits of skin moisture/hydration can be measured using impedance measurements with the Nova DermalPhase Meter. Impedance meters measure changes in skin moisture levels. The outer layers of the skin have different electrical properties. When skin is dry, it conducts electricity poorly. As it becomes more hydrated, increased conductivity results. Thus, changes in skin impedance (related to conductivity) can be used to assess changes in skin hydration. The unit can be calibrated for each test day according to the instrument instructions. Temperature and relative humidity can also be marked. Subjects can be evaluated as follows: they can be equilibrated in a room with defined humidity (eg 30-50%) and temperature (eg 68-72° C.) before measurement. Three independent impedance measurements were taken on each side of the face, recorded and averaged. The impedance meter can be set using T5, which averages the impedance values applied to the face every five seconds. Changes can be reported with statistical variance and significance. Each of the active ingredients disclosed in this specification, combinations of ingredients, or any of compositions having said combinations can be analyzed according to this method.

Analysis of Skin Clarity and Freckle and Age Spot Reduction: Skin clarity and freckle and age spot reduction were evaluated using a Minolta Chromometer. Changes in skin color can be assessed using the a ^* value of the Minolta Chroma Meter to determine the potential for irritation due to product handling. The a ^* value measures the change in skin color in the red area. This was used to determine whether each of the active ingredients disclosed in this specification, the combination of ingredients, or any of the compositions having said combination caused irritation. Measurements can be taken on each side of the face and averaged as values for the left and right faces. Skin clarity can also be measured using the Minolta Meter. The measurement is a combination of Minolta Meter's a ^* , b, and L values, and is related to the brightness of the skin, and corresponds very well to the smoothness and hydration of the skin. Skin assays were performed as above. In one non-limiting aspect, skin clarity can be described as L/C, where C is chroma and is defined as (a ² +b ² ) ^1/2 .

Skin dryness, superficial lines, skin smoothness and complexion analysis: Skin dryness, superficial lines, skin smoothness and complexion can be assessed using clinical scoring techniques. For example, a clinical score for skin dryness can be determined on a five-point standard Kligman scale: (0) the skin is soft and moist; (1) the skin appears normal without visible dryness; (2) the skin feels slightly dry to the touch without visible dryness. (3) the skin felt dry, tough and had a somewhat scaly whitish appearance; and (4) the skin felt very dry, rough and had a scaly whitish appearance. Assessments can be performed independently by two clinicians and averaged.

Skin Tone Clinical Score Analysis: Clinical scoring of skin color can be performed on a ten point analog numerical scale: (10) Smooth even skin, pinkish brown color. No dark, red, or scaly plaques on examination with a hand-held magnifying glass. The microscopic texture of the skin feels very uniform; (7) Observing a uniform skin tone without a magnifying glass. There are no scaly areas, but there are eruptions due to pigmentation or erythema. No rashes larger than 1 cm in diameter; (4) Skin rashes and uneven textures were easily noticed. Few scales. Skin that feels rough to the touch in certain areas; and (1) uneven skin coloration and texture. Scales and eruptions in multiple areas, hypopigmented, red or black spots. Large areas of uneven coloration exceeding 1 cm in diameter. Assessments were performed independently by two clinicians and averaged.

Clinical Score Analysis for Skin Smoothness: Clinical scores for skin smoothness can be analyzed on a ten-point analog numerical scale: (10) smooth, the skin is moist and glistening, and there is no resistance when the finger is slid across the surface; (7) Somewhat smooth, with little resistance; (4) rough, visibly changing, with friction when rubbed; and (1) rough, flaky, uneven surfaces. Assessments were performed independently by two clinicians and averaged.

Analysis of skin smoothness and wrinkle reduction by the method disclosed by Packman et al. (1978): Skin smoothness and wrinkle reduction can also be assessed visually by using the method disclosed by Packman et al. (1978). For example, at each subject visit, the depth, shallowness and total amount of each subject's surface surface line (SFL) can be carefully scored and recorded. Fractions of numbers are obtained by multiplying the quantity factor by the depth/width/length factors. Scores are obtained for the eye area and mouth area (left and right) and added together for the total wrinkle score.

Skin firmness analysis with the Hargens Ballistometer: Skin firmness can be measured with the Hargens Ballistometer, a device that assesses skin elasticity and firmness by dropping a small object on the skin and recording the first two rebound peaks . Ballistometry is a small lightweight detector using a relatively blunt probe (4mm2-contact area). The probe gently penetrates into the skin, resulting in measurements that depend on the properties of the outer layers of the skin, including the stratum corneum and outer epidermis and parts of the dermis.

Skin softness/flexibility analysis with the Gas Bearing Electrodynamometer: Skin softness/flexibility can be assessed using the Gas Bearing Electrodynamometer, an instrument that measures the pressure/tension properties of the skin. Skin viscoelasticity is related to skin moisture retention. Measurements at specific points in the cheek area can be achieved by attaching the probe to the skin surface with double-sided tape. A force of approximately 3.5 gm is applied parallel to the skin surface, precisely measuring skin displacement. The flexibility of the skin can then be calculated and expressed as DSR (Dynamic Spring Rate in gm/mm).

Analysis of the appearance of lines and wrinkles with replicas: The appearance of lines and wrinkles on the skin can be assessed using replicas, which are impressions of the skin surface. Materials such as silicone rubber can be used. Replicas can be analyzed by image analysis. Changes in the visibility of lines and wrinkles can be objectively quantified by forming a subject's face using a silicon replica and analyzing the replica image with a computerized image analysis system. Replicas can be obtained from the eye area and neck area and photographed with a digital camera at low lighting incidence angles. The digital image can be analyzed with an image processing program to determine the areas of the replica covered by wrinkles and fine lines.

Skin Surface Profile Analysis Using the Profilometer/Style Method: The skin surface profile can be measured using the profilometer/stylus method. This involves flashing or dragging the stylus across the surface of the replica. The vertical displacement of the stylus can be entered into a computer via a distance sensor, and after scanning a certain distance of the replica, an analysis of the skin contour can be generated as a two-dimensional surface. This scan can be repeated any number of times along a fixed axis to produce a simulated 3-D image of the skin. Ten random replica sections were obtained using the recording pin technique and combined to generate an average. Values of interest include Ra, which is the arithmetic mean of all roughness (height) values calculated by integrating the profile height with respect to the mean profile height. Rt, which is the maximum vertical distance between the highest peak and lowest valley, and Rz, which is the average peak amplitude minus the average peak height. Values are given as numerical values in mm. The equipment should be standardized before each use by scanning a metal standard of known value. The Ra value can be calculated by the following formula: _Ra = normalized roughness; _lm = transverse (scan) length; and y = absolute value of profile position relative to mean profile height (x-axis).

MELANODERM ^™ Assay: In other non-limiting aspects, the efficacy of any of the active ingredients disclosed in this specification, combinations of ingredients, or compositions having such combinations can be assessed by using a skin analog, such as MELANODERM ^™ . Melanocytes, a type of cell in skin analogs, are specifically contaminated when exposed to L-dihydroxyphenylalanine (L-DOPA), a precursor of melanin. The skin analogue MELANODERM ^™ can be treated with a variety of substrates containing each of the active ingredients disclosed in this specification, a combination of ingredients, or a composition having said combination, or with the substrate alone as a control to process. Alternatively, untreated skin analog samples can be used as controls.

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All of the compositions and/or methods disclosed and claimed herein can be made and carried out without undue experimentation in light of the present disclosure. Although the compositions and methods disclosed herein have been described in terms of preferred embodiments, it will be apparent to those skilled in the art that the compositions described herein can be modified without departing from the concept, spirit and scope of the invention. Variations may be implemented in and/or methods and steps or order of steps of methods. More specifically, it is apparent that some reagents, both chemically and physiologically related, could be substituted for the reagents described herein, while yielding the same or similar results. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.

Claims (24)

1. A composition comprising Mucor miehei extract, Bacillus ferment product, plankton extract and hydrolyzed prickly pear flower extract or nopal flower extract in the preparation for increasing skin regeneration and/or increasing skin mesodermal Use in the production of proteins. 2. The use according to claim 1, wherein the composition comprises: 40% to 60% by weight of water, 0.01% to 1% by weight of Mucor miehei extract, 0.001% to 1% by weight Bacillus fermentation product, 0.001% to 0.1% by weight plankton extract, and 0.01% to 1% by weight hydrolyzed prickly pear flower extract or nopal flower extract. 3. The use according to claim 1, wherein the Mucor miehei extract is an aqueous extract comprising aspartyl-dependent water-soluble enzymes or acid proteases, and wherein the Mucor miehei extract Drugs that exfoliate the skin, increase skin regeneration, and/or increase involucrin production. 4. The use according to claim 1, wherein the Bacillus fermentation product is a fermentation product of Bacillus subtilis, and wherein the Bacillus fermentation product is capable of exfoliating the skin, increasing skin regeneration, and/or increasing involucrin generation. 5. The use according to claim 1, wherein the plankton extract is an aqueous extract comprising exopolysaccharide synthesized by Vibrio alginolyticus, and wherein the plankton extract is capable of conditioning the skin, reducing skin Inflammation, increased skin regeneration, and/or increased involucrin production. 6. The use according to claim 1, wherein said composition comprises nopal flower extract, wherein said nopal flower extract is an aqueous extract, and wherein said nopal flower extract is capable of increasing skin regeneration and and/or increase involucrin production. 7. The use according to claim 1, wherein the composition comprises a hydrolyzed Prickly Pear flower extract, wherein the Prickly Pear flower extract is an aqueous extract, and wherein the hydrolyzed Prickly Pear flower extract Able to exfoliate the skin. 8. The use according to claim 1, wherein the composition further comprises: polysiloxane-11, cyclopentasiloxane, dimethicone, glycerin, PEG-10 dimethicone Oxane, Butylene Glycol, and Bis-PEG-18 Methyl Ether Dimethyl Silane. 9. Use according to claim 8, wherein the composition comprises: 10% to 30% by weight of polysiloxane-11, 5% to 20% by weight of cyclopentasiloxane, 1% by weight to 10% by weight of polydimethylsiloxane, 1% to 10% by weight of glycerin, 1% to 10% by weight of PEG-10 polydimethylsiloxane, 0.5% to 5% by weight of butylene glycol, and 0.5% to 5% by weight of bis-PEG-18 methyl ether dimethylsilane. 10. The use according to claim 8, wherein the composition further comprises: phenoxyethanol, ammonium acryloyldimethyl taurate/VP copolymer, propylene glycol, bio-glucose gum-1, acacia gum extract , hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, squalene, decanediol, sodium citrate, hydroxyethyl cellulose, 1,2-hexanediol, citric acid, glycyrrhizic acid di Potassium, Tocopheryl Acetate, and Xanthan Gum. 11. The use according to claim 10, wherein the composition comprises: 0.1% to 3% by weight of phenoxyethanol, 0.1% to 3% by weight of ammonium acryloyldimethyltaurate/VP copolymer 0.1% to 3% by weight of propylene glycol, 0.1% to 3% by weight of bio-sugar gum-1, 0.1% to 1.5% by weight of acacia gum extract, 0.1% to 1.5% by weight of acrylic acid hydroxyl Ethyl ester/sodium acryloyldimethyl taurate copolymer, 0.1% to 1.5% by weight squalene, 0.01% to 1% by weight decanediol, 0.01% to 1% by weight sodium citrate , 0.01% to 1% by weight of hydroxyethyl cellulose, 0.01% to 1% by weight of 1,2-hexanediol, 0.01% to 1% by weight of citric acid, 0.01% to 1% by weight of Dipotassium glycyrrhizinate, 0.01% to 1% by weight tocopheryl acetate, and 0.01% to 1% by weight xanthan gum. 12. The use according to claim 10, wherein the composition further comprises: hydroxypropyl cyclodextrin, triethanolamine, polysorbate 60, disodium EDTA, sorbitan isostearate, and potassium sorbate. 13. Use according to claim 12, wherein the composition comprises: 0.01% to 1% by weight of hydroxypropyl cyclodextrin, 0.01% to 1% by weight of triethanolamine, 0.01% to 1% by weight % polysorbate 60, 0.01% to 1% by weight disodium EDTA, 0.001% to 0.1% by weight sorbitan isostearate, and 0.001% to 0.1% by weight potassium sorbate . 14. The use according to claim 1, wherein the composition comprises 25% to 80% by weight of water. 15. The use according to claim 1, wherein said composition further comprises: adenosine. 16. The use according to claim 15, wherein the composition comprises 0.001% to 1% by weight of adenosine. 17. The use according to claim 1, wherein the composition further comprises: prickly pear fruit extract. 18. The use according to claim 17, wherein the composition comprises 0.00001% to 0.01% by weight of prickly pear fruit extract. 19. The use according to claim 1, wherein the composition is capable of counteracting skin irritation caused by exfoliating ingredients, exfoliating the skin, increasing skin regeneration and/or increasing involucrin production in the skin. 20. The use of claim 1, wherein the composition is formulated as a facial mask. 21. The use according to claim 20, wherein the composition further comprises an alpha hydroxy acid. 22. Use according to claim 21, wherein the composition comprises 2% to 20% by weight of alpha hydroxy acids. 23. The use according to claim 22, wherein the alpha hydroxy acid is glycolic acid. 24. The use according to claim 1, wherein the composition is an oil-in-water or water-in-oil emulsion.